| 729 DEGs in OXmiR400 plants |
include |
315 upregulated and 414 downregulated genes |
Arabidopsis thaliana |
| 437 DEGs between OXmiR400 and OXrPPR1 |
include |
197 upregulated and 240 downregulated genes |
Arabidopsis thaliana |
| differentially expressed genes between stone cells and cortical parenchyma |
revealed |
424 transcripts upregulated in cortical parenchyma cells |
Picea sitchensis |
| small number of DEGs (241 genes; approximately 1.8% of the whole gene sets) |
were detected between |
the two host species |
Colletotrichum orbiculare |
| PC2 |
explained |
13.4% of the original variance |
Picea sitchensis |
| comparative transcriptome analyses |
were performed between |
stone cells and cortical parenchyma cells |
Picea sitchensis |
| differentially expressed genes (DEGs) |
identified using |
edgeR (glmTreat test) |
Colletotrichum orbiculare |
| comparative transcriptome analyses |
were performed between |
R and S trees |
Picea sitchensis |
| (APC1, PC1, AT5G17480) and PC2 |
accounted for |
63.1% of the original variance |
Picea sitchensis |
| Affymetrix GeneChips |
allowed comparison of |
Rorippa species data with Arabidopsis data |
Rorippa amphibia; Rorippa sylvestris; Arabidopsis thaliana |
| second principal component (PC2) |
separated |
stone cells from cortical parenchyma cells |
Picea sitchensis |
| PC2 |
explained |
14.7% of the original variance |
Picea sitchensis |
| differentially expressed genes between stone cells and cortical parenchyma |
revealed |
486 transcripts upregulated in cortical parenchyma cells |
Picea sitchensis |
| large cluster of upregulated genes |
was found in |
stone cells from R genotype |
Picea sitchensis |
| OXmiR400 transgenic plants |
have |
729 differentially expressed genes (DEGs) relative to WT plants |
Arabidopsis thaliana |
| differentially expressed transcripts in stone cells from R genotype |
were distinct from |
other transcriptomes |
Picea sitchensis |
| (APC1, PC1, AT5G17480) |
explained |
55.1% of the original variance |
Picea sitchensis |
| RNA-seq |
performed on |
root cap, meristem, and elongation zone of WT barley roots |
Hordeum vulgare |
| transcriptome libraries |
included |
two genotypes, two tissue types, and three biological replicates |
Picea sitchensis |
| differentially expressed genes between stone cells and cortical parenchyma |
revealed |
1129 transcripts significantly upregulated in stone cells |
Picea sitchensis |
| significantly differentially expressed genes |
are identified by |
RNA-Seq differential expression analysis |
Gossypium hirsutum |
| Principal component analysis (PCA) of variation |
revealed |
reproducible separation of transcriptome clusters between genotype and time points |
Picea sitchensis |
| COT samples |
exhibit |
higher variance throughout time course |
Lepidium sativum |
| PC2 |
separated |
TP1, TP2, TP3 from TP4 |
Picea sitchensis |
| principal component analysis (PCA) |
revealed |
reproducible separation between genotype and cell-type |
Picea sitchensis |
| RIP RNA samples |
were submitted to |
RNAseq |
Arabidopsis thaliana |
| transcriptome differences |
separated |
R and S genotypes |
Picea sitchensis |
| differentially expressed transcripts |
showed clear separation between |
genotypes and cell-type transcriptomes |
Picea sitchensis |
| Functional categorization analysis (using Pageman) |
was performed for |
transcripts changing in abundance in response to stress for both (AOX1A, ATAOX1A, AtHSR3, HSR3, AT3G22370) and rpoTmp plants |
Arabidopsis thaliana |
| replicates |
were grouped together in |
four distinct clusters |
Picea sitchensis |
| Pearson's correlation coefficient |
was approximately |
0.99 |
|
| retooled INTACT |
can enable high-resolution monitoring of |
nuclear transcriptome |
Oryza sativa L. |
| coverage for these RNAs |
was more evenly distributed along |
transcription unit |
Oryza sativa |
| DEGs upregulated in OXrPPR1 |
are also differentially expressed but in opposite direction in |
OXmiR400 plants |
Arabidopsis thaliana |
| one biological replicate for R genotype in TP1 |
did not cluster with |
other samples |
Picea sitchensis |
| 454 pyrosequencing |
compares |
expression profiles in each cell type |
Hieracium praealtum |
| average distribution of RNA-seq reads across all individual transcripts detected in nuclear and poly(A) + RNA with >5 rpkM |
illuminated |
bias in RNA-seq reads near the 5′ end of nuclear transcripts in contrast to the more equally distributed reads across the entire transcription unit in poly(A) + RNA |
Oryza sativa |
| transcriptional signatures of enlarged aposporous initial (AI) cells |
were identified by comparing with |
apomictic ovaries at specific developmental stages |
Hieracium praealtum |
| microarray studies and statistical analyses |
described in detail in |
Supplementary Data |
Arabidopsis thaliana |
| cluster analysis of transcript abundance patterns |
showed |
five clearly distinct clusters |
Lepidium sativum |
| DAVID GO cluster tool |
is |
widely used transcriptome data interpretation tool |
Arabidopsis thaliana |
| gl3–sst (SIM, AT5G04470) trichomes |
were used to generate probes for |
hybridization to Affymetrix (ATH1, AT4G32980) GeneChips |
Arabidopsis thaliana |
| subsampling of the libraries |
confirmed that |
each library had reached a saturation of transcripts identified as present in each population |
Oryza sativa |
| differential gene expression analysis in aposporous initial (AI) and somatic ovule (SO) cells and early aposporous embryo (EAE) sacs |
was conducted using |
Illumina sequencing platform |
Hieracium praealtum |
| soybean lincRNAs |
were available for analysis with |
6,018 loci |
Glycine max |
| poly(A) + RNA |
had about |
16,500 protein-coding transcripts detected |
Oryza sativa |
| young stems of LA3560 (Wo) and non-woolly segregants (WT) |
were utilized to construct |
cDNA libraries |
|
| INTACT |
was used to evaluate |
complete nuclear transcriptome by RNA-seq |
Oryza sativa |
| remaining reads |
were mapped to |
exons with ≤2 mismatches to the Nipponbare genome (IRGSP-1.0.30) |
Oryza sativa |
| strand-specific RNA-Seq data |
were not available for |
sperm cells and young (AtEMF2, CYR1, EMF2, VEF2, AT5G51230) mutant panicles |
Oryza sativa |
| high-throughput technologies |
offer opportunity for |
coding and noncoding transcript detection and quantification |
|
| nuclear RNA samples |
had greater proportions of |
introns and 5′ UTRs (flanking regions) |
Oryza sativa |
| 17 genes |
showed |
expression differences greater than 100-fold |
Zea mays |
| Red and blue boxes |
indicate |
up-regulated and down-regulated transcripts, respectively, according to the color scale for (AIRP3, AtAIRP3, LOG2, AT3G09770) fold changes |
Arabidopsis thaliana |
| most fold changes |
showed that there was less than 10% difference in the fold change magnitude between |
the two mutant backgrounds |
Arabidopsis thaliana |
| dominance of unique changes in the double mutant |
means that |
the transcriptome changes at a functional level were defined by these unique responses |
Arabidopsis thaliana |
| RNA-seq analysis |
identified |
622 other genes differentially expressed in sulki1-8 versus Ler/ (ATKAS2, FAB1, KAS2, AT1G74960) NIL that did not show significant expression differences in versus Ler/ NIL |
Arabidopsis thaliana |
| unique response in (AOX1A, ATAOX1A, AtHSR3, HSR3, AT3G22370) rpoTmp double mutants |
was not a sum of the changes observed in |
each single mutant |
Arabidopsis thaliana |
| nuclear RNA libraries |
had somewhat greater variation than |
poly(A) + RNA libraries |
Oryza sativa |
| laser-capture microdissection in conjunction with 454 pyrosequencing |
was used to examine |
transcripts in enlarging AI cells, early aposporous embryo (EAE) sacs, and somatic ovule (SO) cells |
Hieracium praealtum |
| transcriptional signatures of enlarged aposporous initial (AI) cells |
were identified by comparing with |
deletion mutants that have lost the capacity to form AI cells |
Hieracium praealtum |
| Haberlea rhodopensis transcriptome reads |
were pseudoaligned to the assembly using |
kallisto program |
Haberlea rhodopensis |
| differentially expressed (DE) genes |
were grouped into |
simple expression clusters based on point of maximal expression |
Xerophyta humilis |
| PCA analysis |
grouped |
Q2;1, Q3;1, and Q3;2 close together and separate from wild-type, Q1;1, and Q2;2 |
|
| Three-way principle component analysis (PCA) |
was carried out for |
all microarray gene chips, showing a clear separation on PCA1 (64.9%) for (AOX1A, ATAOX1A, AtHSR3, HSR3, AT3G22370) and rpoTmp under MLD conditions |
Arabidopsis thaliana |
| principal component analysis (PCA) |
identifies |
eigenvectors with greatest influence on sample variance |
Lepidium sativum |
| mRNAs with introns |
had few nuclear RNA reads map to |
intervening regions |
Oryza sativa |
| Three nucleus-enriched transcripts |
illustrate |
read mapping pattern in genome browser views: DELLA protein SLENDER LEAF (RICE1, AT3G11770) (SLR1), ARG DECARBOXYLASE, and ABC TRANSPORTER B FAMILY MEMBER9 |
Oryza sativa |
| overlap in genes changing in the (AOX1A, ATAOX1A, AtHSR3, HSR3, AT3G22370) rpoTmp mutants compared with and rpoTmp |
was very small |
|
Arabidopsis thaliana |
| mRNAs |
were highly enriched in |
nucleus as compared to the poly(A) + RNA pool |
Oryza sativa |
| read coverage averaged for all poly(A) + RNAs |
uncovered |
modest 5′-end bias and a more pronounced 3′-end bias |
Oryza sativa |
| strand-specific RNA-seq data from seedling and drought-stressed samples |
enabled extraction of strand information for |
464 lincRNA loci |
Oryza sativa |
| most available transcriptome data interpretation tools |
are based on |
enrichment or clustering analysis |
Arabidopsis thaliana |
| RNA-seq |
was performed on |
X. humilis leaves harvested at five relative water contents (RWCs) during vegetative desiccation |
Xerophyta humilis |
| RNA sequencing of 240 lettuce accessions |
generated |
expression data for 22,039 genes |
Lactuca sativa |
| correlation matrix heatmap |
indicated that transcriptome responses changed substantially between |
8 and 16 hpi in all three accessions |
Brachipodium distachyon |
| 3′ mRNA-seq-based transcriptome analysis |
identified |
1164 differentially expressed genes (DEGs) between BdWRKY38-ox and wild-type Bd21 |
Brachipodium distachyon |
| principal component 2 (PC2) |
separates |
field cold acclimation (F-CA) from artificial cold acclimation (A-CA) and artificial cold acclimation with UVB (A-CA + UVB) |
|
| obtained RNA-seq reads |
were mapped to |
Bd21 reference genome |
Brachipodium distachyon |
| genes showing no or very low expression |
are removed using |
HTSfilter R package |
Petunia axillaris; Petunia exserta; Petunia parodii |
| DYGs in Bd3-1 |
overlapped with |
DYGs in Bd21 |
Brachipodium distachyon |
| RK16331–13 line |
subjected to |
three independent hybridizations |
Oryza sativa |
| two-module discovery pipeline |
allows one to make good use of |
all available plant-microbe interaction transcriptome data |
|
| 20 WRKY genes |
were identified as |
GRN hubs |
Brachipodium distachyon |
| Controls and Phe-treated flowers |
shared high correlation and formed one cluster depicting |
small transcriptional changes |
Chrysanthemum morifolium |
| sequencing |
yielded |
~54 to ~95 million read pairs per library |
Lotus japonicus |
| WGCNA method |
was used to classify |
expression patterns of transcription factors |
Zea mays |
| predictor performance |
was slightly better in |
leaf and root samples |
Arabidopsis thaliana |
| bud stage (0 DAP) |
had |
240 DEGs |
Aethionema arabicum |
| 3' mRNA sequencing |
was performed on |
mesophyll, bundle sheath and veinal cells |
Oryza sativa |
| ten WRKY genes |
were hubs in |
GRNs of the resistant accessions |
Brachipodium distachyon |
| BdWRKY36 and BdWRKY38 |
were shared between |
Bd3-1 and Tek-3 |
Brachipodium distachyon |
| differentially expressed genes (DEGs) |
identified in |
Experiment II (A-NA versus A-CA) |
|
| gene regulatory network (GRN) analysis |
was performed in |
Bd3-1, Tek-3 and Bd21 |
Brachipodium distachyon |
| BdWRKY25 |
was specific to |
Bd3-1 |
Brachipodium distachyon |
| gene expression samples |
cluster more similarly than by |
species |
Petunia axillaris; Petunia exserta; Petunia parodii |
| sRNA sequencing |
combined with |
mRNA/lncRNA sequencing |
Arabidopsis thaliana |
| RNA-seq libraries |
separated primarily by |
experiment (tissue of origin) |
Xerophyta humilis |
| GRNs in Bd21, Bd3-1 and Tek-3 |
were composed of |
496, 692 and 532 nodes with 10, 8 and 16 hub WRKY genes, respectively |
Brachipodium distachyon |
| leaf tissue samples at higher water contents (100%, 80% and 60% RWC) |
showed clear separation from |
samples during later stages of desiccation (40% and 5% RWC) |
Xerophyta humilis |
| 118 genes |
were expressed at steady-state in |
both BdWRKY38-ox and wild-type Bd21 |
Brachipodium distachyon |
| The expression of (PRLIP2, AT5G24200) |
was still 26-fold higher in |
complementation line #2 than in the wild type |
Arabidopsis thaliana |
| 3' mRNA sequencing |
obtained from each cell type |
24–36 million reads from four or five biological replicates |
Oryza sativa |
| Biological replicates of control infected flowers |
showed high correlation and grouped far apart in |
principal component analysis (PCA) |
Chrysanthemum morifolium |
| DEGs |
were clustered into |
seven semi-distinct groups |
Marchantia inflexa |
| predictor |
performed just moderately well on |
single-cell RNA-Seq samples |
Arabidopsis thaliana |
| rice seedling transcriptome |
is analyzed by |
STAIR pipeline |
Oryza sativa L. ssp. japonica |
| mutant anther samples at stages 8a–9 |
are obviously separated from |
corresponding WT anther samples in principal-component analysis |
Zea mays |
| KEGG pathways |
enriched in |
Experiment I (F-NA versus F-CA) |
|
| RNA sequencing (RNA-seq) analysis |
was performed using |
bHLH6 OV, (ATSPX4, SPX4, AT5G15330) and WT plants |
Oryza sativa |
| AtGenExpress project |
measured transcriptomes of |
all major Arabidopsis tissues using microarrays |
Arabidopsis thaliana |
| 24,545 RNA-Seq runs |
originated from |
1326 NCBI SRA studies |
Arabidopsis thaliana |
| predictor |
was found to produce highly accurate predictions for |
different samples in most studies |
Arabidopsis thaliana |
| three growth-retarded lines (group 2) |
cluster close together in |
hierarchical clustering family tree |
|
| WT and ms33-6038 anther samples at stages 5–7 |
are closer in distance in |
principal-component analysis |
Zea mays |
| fruit under different temperature treatments |
were clearly separated by |
principal component analysis (PCA) |
Prunus persica |
| RNA sequencing (RNA-seq) |
was performed on |
wild type (WT) and osnam-1 |
Oryza sativa |
| (PRLIP2, AT5G24200) expression levels in the wild type |
were very low, so |
(SARD1, AT1G73805) was tested rather than (PRLIP2, AT5G24200) |
Arabidopsis thaliana |
| APX01 and (PrxIIE, AT3G52960) |
showed rather high expression in |
cotyledons |
|
| B− Myc versus NoMyc contrast |
shows highest number of |
4514 differentially expressed transcripts |
Lotus japonicus |
| RNA extracted from roots per segregating wild type and homozygous (COI1, AT2G39940) or (AOS, CYP74A, DDE2, AT5G42650) mutants |
were used to construct libraries for |
Illumina sequencing |
Arabidopsis thaliana |
| PCA of individual genotypes |
revealed |
highly variable expression across genotypes |
Marchantia inflexa |
| different tissues and/or conditions |
were included to capture |
wide range of transcripts |
Amaranthus cruentus |
| (PRLIP2, AT5G24200) (PATHOGENESIS-RELATED LIPASE2 ) |
was about 50-fold more highly expressed in |
coi1-t than in the two wild-type lines and the (AOS, CYP74A, DDE2, AT5G42650) mutant |
Arabidopsis thaliana |
| 371 genes with increased relative transcript abundance in shoots of (ATSPX4, SPX4, AT5G15330) |
also showed increased transcript abundance in shoots of |
bHLH6 OV plants |
Oryza sativa |
| 456 genes with decreased relative transcript abundance in shoots of (ATSPX4, SPX4, AT5G15330) |
also showed decreased relative transcript levels in shoots of |
bHLH6 OV plants |
Oryza sativa |
| highly accurate and tissue-independent gene expression predictor |
can be applied universally to |
most Arabidopsis bulk RNA-Seq samples |
Arabidopsis thaliana |
| Enhanced expression of (ATICS1, EDS16, ICS1, SID2, AT1G74710) |
was also observed in |
coi1-1, but not in coi1-16 |
Arabidopsis thaliana |
| presence of endobacteria |
led to |
modulation of 152 genes |
Gigaspora margarita |
| eight gene modules with tissue-preferential expression |
derived from |
AtGenExpress project |
Arabidopsis thaliana |
| snRNA-seq |
suffer less from |
transcriptional artifacts |
|
| dynamically expressed genes (DYGs) |
were identified in |
Bd21, Bd3-1 and Tek-3 |
Brachipodium distachyon |
| B− Myc and B+ Myc samples |
partially discriminated by |
second principal component (PC2, y-axis) |
Lotus japonicus |
| Ca Gg presence |
explains |
4% variance explained in plant transcriptome |
Lotus japonicus |
| 60 RNAseq libraries |
collectively spanned |
~1.3 billion reads |
Marchantia inflexa |
| 8012 genes |
were |
upregulated in (EDA33, GT140, IND, IND1, AT4G00120) versus DEH samples |
Aethionema arabicum |
| genes whose expression was significantly induced (fold change >2, FDR < 0.01) in mutants compared with wild-type |
were selected from |
triplicate RNA-seq data |
|
| RNA-seq raw data |
were downloaded and processed via |
uniform purpose-built pipeline |
Arabidopsis thaliana |
| isolation of nuclei |
aids much broader applicability of |
snRNA-seq method to plant tissues |
|
| single-cell genomics |
enables relating |
genes to functions, structures, and phenotypes |
|
| bacterial genes |
not detected in |
B+ Myc samples |
Lotus japonicus; Candidatus Glomeribacter gigasporarum |
| isolation of nuclei |
could offer an alternative for |
single-nucleus RNA-sequencing (snRNA-seq) |
|
| expression pattern |
was |
independent of whether plants were mock-treated or infected |
Arabidopsis thaliana |
| (AT2G05420) |
was not affected by |
(COI1, AT2G39940) mutation |
Arabidopsis thaliana |
| (AtDOB11, AT5G54450) |
had expression so low that |
no specific PCR product was detected |
Arabidopsis thaliana |
| genes differentially expressed in coi1-t |
were compared to |
both wild types and the (AOS, CYP74A, DDE2, AT5G42650) mutant |
Arabidopsis thaliana |
| principal component analysis (PCA) |
examined |
overall variation in genome data |
Picea abies |
| single-cell transcriptome profiling |
enables observing |
changes in each cell's transcriptome and among its neighborhoods |
|
| increased expression of (ATPGMP, PGM, PGM1, STF1, AT5G51820) and (PRLIP2, AT5G24200) |
was confirmed in |
coi1-1 and the temperature-sensitive coi1-16 mutant |
Arabidopsis thaliana |
| principal component analysis (PCA) |
was conducted on |
global gene expression |
Marchantia inflexa |
| (APC1, PC1, AT5G17480) |
explained |
66.35% of the total variance in gene expression |
Marchantia inflexa |
| baseline samples |
exhibited similar gene expression patterns to |
one another |
Marchantia inflexa |
| non-diurnal genes |
were expressed in |
more condition-specific manner than diurnally expressed genes |
Skeletonema robusta |
| 24-kDa oleosin isoform |
down-regulated with fold change of |
−5.3-fold |
Glycine max |
| 3 lines with insertion in peroxidase genes |
were chosen because of |
strong representation of peroxidases in cDNA library |
Arabidopsis thaliana |
| predictor model |
was constructed with |
RNA-Seq datasets available from SRA as of October 2019 |
Arabidopsis thaliana |
| spatial transcriptomics |
provides |
precise localization of mRNAs within cells of tissues |
|
| SYSTEMIC ACQUIRED RESISTANCE DEFICIENT1 (SARD1, AT1G73805) |
was chosen as a second gene and again found |
no influence of the jazD genotype |
Arabidopsis thaliana |
| RNA-sequencing (RNA-seq) |
was used for |
morph-specific sampling |
Aethionema arabicum |
| flower samples |
are more similar in their transcriptional profile to |
bud samples than to fruit samples |
Aethionema arabicum |
| quantitative PCR |
was used to quantify |
expression of three peroxidases |
|
| transcript extraction |
has been from |
homogenized amalgam of various tissues and cell types |
|
| veinal cells |
had an average of |
13,648 transcripts detected |
Oryza sativa |
| expression |
was investigated in |
endosperm cap, non-micropylar endosperm, radicle, and cotyledons |
|
| eight WRKY genes |
were hubs in |
both susceptible and resistant accessions |
Brachipodium distachyon |
| Affymetrix soybean array |
underlying dataset derived from |
public soybean EST project |
Glycine max |
| hydrophobic seed protein precursor |
down-regulated with fold change of |
−18.7-fold |
Glycine max |
| (PRLIP2, AT5G24200) expression |
was not affected in |
35S:JAZ1Δ3A:GUS plants |
Arabidopsis thaliana |
| presence of the AMF |
explains |
50% variance explained in plant transcriptome |
Lotus japonicus |
| majority of genes |
had negative loading scores on |
(APC1, PC1, AT5G17480) |
Marchantia inflexa |
| replicate RNA-seq samples |
clustered tightly by |
MORPH |
Aethionema arabicum |
| 16 243 genes |
were found to be differentially expressed in |
(EDA33, GT140, IND, IND1, AT4G00120) versus DEH samples |
Aethionema arabicum |
| higher level of rhythmicity of Skeletonema robusta's transcriptome |
might be explained by |
longer duration of the time series |
Skeletonema robusta |
| B− Myc versus NoMyc and B+ Myc versus NoMyc contrasts |
showed |
prevalence of downregulated genes |
Lotus japonicus |
| differential gene expression |
was evaluated using |
DESeq2 R package |
Lolium rigidum |
| computational tools for data analysis |
are required for |
comprehensive and quantitative transcript profiling |
|
| transcriptome dynamics throughout endosperm development in T. urartu |
were revealed using |
RNA-seq |
Triticum urartu |
| Lotus japonicus roots colonized by fungal line with bacteria (B+) |
analyzed by |
deep dual-mRNA sequencing |
Lotus japonicus; Gigaspora margarita |
| direct comparison B+ Myc versus B− Myc |
exclusive of |
22 genes |
Lotus japonicus |
| transcript levels of (PRX16, AT2G18980) |
were significantly higher in endosperm caps than in |
radicles |
|
| high-throughput RNA-seq |
identifies |
non-protein-coding transcripts |
|
| down-regulated transcripts |
include |
high-proline proteins |
Glycine max |
| repetitive proline-rich protein |
down-regulated with fold change of |
−8.0-fold |
Glycine max |
| SSH-technique |
used to construct |
subtractive cDNA library |
|
| transcripts of APX01 and (PrxIIE, AT3G52960) |
were both already present in |
dry radicles and endosperm caps |
|
| nine libraries |
obtained from |
three conditions with three biological replicates each |
Lotus japonicus |
| B+ Myc versus NoMyc contrast |
shows |
3147 differentially expressed transcripts |
Lotus japonicus |
| B− Myc versus NoMyc and B+ Myc versus NoMyc contrasts |
share |
2447 transcripts |
Lotus japonicus |
| Handa et al. (2015) study |
identified |
3641 DEGs |
Lotus japonicus; Rhizophagus irregularis |
| 24-kDa oleosin isoform (partial) (clone P24/89) |
down-regulated with fold change of |
−3.9-fold |
Glycine max |
| Alstroemeria petal ESTs |
almost doubling |
number of ESTs reported in previously published work |
Alstroemeria |
| cDNA microarray |
analysed |
transcript profiles of Vitis vinifera cultivars Regent and Trincadeira |
Vitis vinifera |
| alternative splicing (AS) |
was revealed by |
mRNA sequencing (RNA-Seq) |
Phyllostachys edulis |
| (ATPGMP, PGM, PGM1, STF1, AT5G51820) and (PRLIP2, AT5G24200) expression |
was as high in |
coi1-t (AOS, CYP74A, DDE2, AT5G42650) as in coi1-t |
Arabidopsis thaliana |
| RNA sequencing |
identified |
differentially expressed genes (DEGs) |
|
| soybean 24-kDa oleosin isoform |
down-regulated with fold change of |
−7.5-fold |
Glycine max |
| calcium-binding EF hand protein (caleosin) |
up-regulated with fold change of |
4.1-fold |
Glycine max |
| hydroxyproline-rich glycoprotein (sbHRGP2) mRNA, 3' end |
up-regulated with fold change of |
3.2-fold |
Glycine max |
| 84 of 229 candidates |
detected in |
previous transcriptome analysis of cotton upon V. dahliae inoculation |
Gossypium hirsutum |
| differential bri 3 transcriptome |
only ~30% is contained in |
differential (BRX, NLM9, AT1G31880) or (OPS, AT3G09070) transcriptomes |
Arabidopsis thaliana |
| At-TAX |
is resource for |
transcript identification |
Arabidopsis thaliana |
| bri 3- RESCUED transcriptome |
is |
more distant from Col-0 (n = 142; p < 0.01) than from bri 3 |
Arabidopsis thaliana |
| analysis of the gerbera floral transcriptome using EST sequencing |
yielded no tags for |
a second GGLO gene |
|
| RNA-seq analysis |
yielded |
7–17 million reads per replicate |
Medicago truncatula |
| novel intergenic transcript units (TUs) |
obtained from |
high-throughput sequencing from control leaves (CL) and drought leaves (DL) |
Populus trichocarpa |
| transcripts from intergenic regions |
identified as |
transcript units (TUs) |
Populus trichocarpa |
| genes for stress acclimation |
are under-represented among |
upregulated genes in P-depletion treatment |
Medicago truncatula |
| RNA-Seq method |
has limitations in |
determination of full structure and strand information |
|
| 28-kDa protein |
down-regulated with fold change of |
−3.0-fold |
Glycine max |
| differentially expressed genes (DEGs) |
include |
less than 30 differentially expressed transcription factors (DETFs) |
Marchantia polymorpha |
| RNA-seq analysis |
applied to |
multiple treatment samples |
Rorippa aquatica |
| each tissue-specific expression profile |
included |
most transcripts detected in any tissue (range: 77-90% of core transcripts) |
Panicum hallii |
| Gene Ontology (GO) overrepresentation analysis |
revealed |
overrepresentation of energy-demanding pathways in down-regulated genes |
Rorippa amphibia; Rorippa sylvestris |
| replicates |
were generally grouped together |
by genotype and time point |
Picea sitchensis |
| other 15% of reads |
contained |
regions of high expression within intergenic regions |
Populus trichocarpa |
| DEGs between OXmiR400 and OXrPPR1 plants |
include |
437 significantly differentially expressed genes |
Arabidopsis thaliana |
| OXrPPR1 transgenic plants |
have |
409 differentially expressed genes (DEGs) relative to WT plants |
Arabidopsis thaliana |
| microarray analysis |
provides |
approximately 80% genome coverage |
Arabidopsis thaliana |
| expression profile of chickpea genes in root tissue |
most distinct from |
other tissues analysed |
Cicer arietinum |
| genes |
exhibited preferential expression in |
particular tissue |
Cicer arietinum |
| genes |
showed preferential or specific expression in |
root and flower tissues |
Cicer arietinum |
| RNAseq analysis |
analyzed for |
significant transcript enrichment |
Arabidopsis thaliana |
| inclusion of outlier replicate |
did not impact |
overall statistical analyses |
Picea sitchensis |
| differentially expressed genes |
are defined as |
genes with twofold expression difference and P-value < 0.05 |
Oryza sativa L. ssp. Japonica |
| Arabidopsis (ATH1, AT4G32980) GeneChip arrays |
was used for |
microarray experiments |
Rorippa amphibia; Rorippa sylvestris |
| RNA-deep sequencing |
was based on |
genomic analysis |
|
| global expression profiles |
were investigated using |
Illumina RNA sequencing approach |
Oryza sativa |
| P-depletion nodule tissue |
expressed |
14,330 genes |
Medicago truncatula |
| P-depletion treatment |
results in |
1,140 differentially expressed genes |
Medicago truncatula |
| bri 3 and bri 3- RESCUED transcriptomes |
show |
even fewer differences (n = 87; p < 0.01) than between Col-0 and bri 3 |
Arabidopsis thaliana |
| Arabidopsis genome (TAIR10 annotation) |
is used for |
sequence alignment |
Arabidopsis thaliana |
| ratio of annotated exons to polyA(+) transcripts detectable on tiling arrays |
appears to be much higher in |
Arabidopsis than in some other organisms |
Arabidopsis thaliana |
| RNA sequencing (RNA-seq) |
performed on |
bri 3, bri 3- RESCUED, and Col-0 wild-type root tips |
Arabidopsis thaliana |
| genes differentially expressed between bri 3 and bri 3- RESCUED root tips |
only ~6% (n = 5) have been described as |
brassinosteroid-responsive, high-confidence (BZR1, AT1G75080) targets |
Arabidopsis thaliana |
| K-means clustering |
was undertaken for |
DEGs |
Raphanus sativus |
| mutants with reduced PRC2 activity |
used to profile |
expression of transposable elements (TEs) |
Cyanidioschyzon merolae; Marchantia polymorpha; Phaeodactylum tricornutum |
| mapping onto a reference database |
was chosen to overcome |
problem of orthology establishment assumptions |
|
| RNA-seq |
sequences |
polyadenylated RNAs |
|
| RNA sequencing reads |
are first assembled into |
transcripts |
Arabidopsis thaliana |
| nuclear RNA pool |
was enriched for |
nascent transcripts |
Oryza sativa |
| nuclear RNA and poly(A) + measures of transcript abundance |
yield |
distinct results |
Oryza sativa |
| RNA transcripts from rind tissues of internodes |
deep sequenced from |
greenhouse-grown Mo17 and (B73, CHL6, CNX, CNX1, SIR4, AT5G20990) plants |
Zea mays |
| some poly(A) + -enriched mRNAs |
could be those from |
cells that do not express the p35S |
Oryza sativa |
| chickpea lincRNAs |
were available for analysis with |
2,248 loci |
Cicer arietinum |
| RNA-seq data |
generated from |
libraries prepared with RNA isolated from different tissues/organs |
Cicer arietinum |
| group of genes with ubiquitous expression |
does not significantly change based on |
CPM filtering |
Pinus pinaster |
| nonpolyadenylated RNAs in Arabidopsis |
make |
more limited contribution to the Arabidopsis transcriptome |
Arabidopsis thaliana |
| root tips of 7-d-old p35S:NTF2 seedlings |
were used for |
RNA-seq analysis |
Oryza sativa |
| 14,264 transcripts above the 5 rpkM threshold in both populations |
had |
6,234 significantly enriched in one or the other population (nuclear, 3,152; poly(A) + , 3,082; |log 2 fold difference| >1, FDR ≤ 0.01) |
Oryza sativa |
| genes upregulated in stone cell transcriptomes |
includes |
1049 contigs uniquely differently expressed in R genotype |
Picea sitchensis |
| whole plants |
were sampled for |
RNA sequencing |
Oryza sativa L. ssp. Japonica |
| dormant seed |
indicated that its steady state pool of transcripts differed most dramatically from |
other tissues analyzed |
Sorghum bicolor |
| non-vernalized wild-type and ezl1-1 plants |
were grown to third-leaf stage and RNA isolated from |
entire shoot in the middle of photoperiods |
Brachypodium distachyon |
| ezl1-1 plants |
have nearly 1400 genes differentially expressed in relative to |
non-vernalized wild-type plants |
Brachypodium distachyon |
| mRNA sequencing |
allowed identification of |
17,000 transcripts |
Arabidopsis thaliana |
| RNA-sequencing (RNA-seq) |
has greatly facilitated |
study of gene expression in non-model organisms |
|
| Remaining cultivar-specific transcripts |
were also transcribed from genes that |
varied in copy number between cultivars |
Vitis vinifera |
| expression correlation in diploid species |
was followed by clustering by |
tissue (endosperm and root) |
|
| methodology |
involved assembling |
overlapping RNA sequences (contigs), to construct Unigenes corresponding to the transcriptome of HS and NTSR plants respectively |
Alopecurus myosuroides |
| TranSeq method |
could efficiently discriminate between |
highly similar members of gene families |
Solanum lycopersicum |
| 3′-end sequencing methods |
may be able to detect differences in |
expression of gene family members |
|
| sequence divergence in 3′UTR |
allows |
TranSeq to outcompete TruSeq in differentiating gene expression patterns |
Solanum lycopersicum |
| standard RNA-seq methods with sequence reads covering entire transcript |
has weakness of erroneous assignment of reads in-between |
highly related sequences such as members of same gene family |
|
| RNA sequencing |
was performed on |
seed coats harvested at 2-day intervals throughout development |
Cleome hassleriana |
| q distribution |
remained skewed toward |
maternal overrepresentation |
Arabidopsis thaliana |
| TopHat2 |
is used to align |
sequencing reads to Arabidopsis genome |
Arabidopsis thaliana |
| 462 genes |
showed |
expression differences 5-fold or greater |
Zea mays |
| at least 16 transcription factors |
displayed |
5-fold difference in expression |
Zea mays |
| portion of the transcript mapping to introns, coding regions and untranslated regions (UTRs) |
was evaluated |
in nuclear and poly(A) + RNA |
Oryza sativa |
| pollen transcriptome |
has been found to be most highly divergent according to |
MPSS (massively parallel signature sequencing of transcripts) |
|
| isolated wild-type trichomes |
were used for |
variety of analyses including preliminary transcriptome analysis using Affymetrix (ATH1, AT4G32980) GeneChip |
Arabidopsis thaliana |
| mutants with reduced PRC2 activity |
used to profile |
expression of protein-coding genes (PCGs) |
Cyanidioschyzon merolae; Marchantia polymorpha; Phaeodactylum tricornutum |
| rescue of bri 3 root phenotypes by CVP2::BRI1-CITRINE |
involves |
rather limited set of transcriptional changes |
Arabidopsis thaliana |
| rarefaction analysis of all tissues |
was used to estimate |
minimum sequencing requirements |
Panicum hallii |
| RNAs corresponding to a subset of pseudogenes |
are much more abundant in |
polyA(±) fraction |
Arabidopsis thaliana |
| filtered set of non-redundant singletons |
used for |
annotation and expression analysis |
|
| chickpea-specific genes |
exhibited tissue-preferential and/or stress-responsive expression |
10% |
Cicer arietinum |
| tiling arrays |
address expression difference between |
two conditions |
|
| tiling arrays |
allow detection of |
all transcripts irrespective of annotation status |
|
| LOC_ genes |
were used for |
further analysis |
Oryza sativa |
| read count filtering process |
revealed |
existence of considerable number of transcripts with low expression |
Pinus pinaster |
| stage-specific transcripts |
were transcribed from genes located predominantly in |
distal chromosome regions |
Vitis vinifera |
| 106 variable-shared clusters |
comprised |
4,876 genes representing 26.9% of all modulated genes |
Vitis vinifera |
| differentially expressed genes |
were classified based on |
level of haplotype sharing between cultivars |
Vitis vinifera |
| 7627 A and D subgenome homoeologs in AADD |
were analyzed using |
correlation dendrogram |
|
| cDNA libraries |
were prepared in biological triplicates from |
Peldon and Rothamsted |
Alopecurus myosuroides |
| (GL2, AT1G79840) |
was down-regulated in |
its respective mutants as compared with wild-type controls |
Zea mays |
| differential (BRX, NLM9, AT1G31880) transcriptome |
is nearly 90% contained in |
differential (OPS, AT3G09070) transcriptome |
Arabidopsis thaliana |
| expression correlation in AADD allotetraploid |
was followed by clustering by |
tissue (endosperm and root) |
|
| 78 host-induced secreted proteins (SPs) identified by Tisserant et al. |
could confirm most |
62 secreted proteins (SPs) |
Rhizophagus irregularis |
| TranSeq data |
provide |
accurate expression measurements of specific gene family members |
|
| gene family members possessing high sequence similarity |
can be accurately measured by |
TranSeq data |
|
| PC4 |
captured variance between |
sampled tissues |
Solanum lycopersicum |
| (EMA1, GIR1, SAD2, URM9, AT2G31660) OE hairy roots |
were compared with |
control hairy roots |
Medicago truncatula |
| hyperosmotic stress |
combined with |
RNA sequencing (RNA-seq) analysis |
Arabidopsis thaliana |
| cell-specific fluorescent marker lines |
enable analysis of |
transcriptome of different cell types in roots |
|
| 96% of contigs |
detected at |
0.5 million reads |
|
| tissue type |
had large influence on |
transcriptional profile clustering |
Sorghum bicolor |
| PC2, PC3 and PC4 |
showed similar trend between |
TranSeq and TruSeq methods |
Solanum lycopersicum |
| gene expression levels |
were stored in |
two matrices of nine conditions each |
Solanum lycopersicum |
| TranSeq |
when examining gene families, shows |
only one gene per family expressed to significant level while others with sequence similarity displayed much lower expression |
Solanum lycopersicum |
| all known cloned glossy genes |
exhibited |
significant DE in at least one mutant versus wild-type comparison |
Zea mays |
| principal component analysis (PCA) |
separated |
two inbred lines |
Raphanus sativus |
| leaf tissue |
required |
10.5 million aligned reads to detect 90% of transcripts |
Panicum hallii |
| Bowtie2 |
is used to align |
sequencing reads to Arabidopsis genome |
Arabidopsis thaliana |
| Cuffnorm |
is used for |
RNA-Seq normalization |
Arabidopsis thaliana |
| Cumme(R)bund suite |
is used for |
transcriptome quality control and comparison |
Arabidopsis thaliana |
| transcriptome data |
assigned accession number |
GSE14663 |
Arabidopsis thaliana; Oryza sativa |
| plant-microbe interaction transcriptome data |
can be generated from |
single-time-point experiments |
|
| plant-microbe interaction transcriptome data |
can be generated from |
time-course experiments |
|
| KEGG pathways |
enriched in |
Experiment II (A-NA versus A-CA) |
|
| principal component analysis (PCA) |
clearly separated |
treated from control samples |
Rorippa amphibia; Rorippa sylvestris |
| S. foetida EST sequences |
contains |
26,083 leaf EST assemblies |
Steculia foetida |
| poly(A) + RNA libraries |
yielded highly consistent results with |
R 2 ≥ 0.978, Pearson correlation |
Oryza sativa |
| 23 gene models |
had higher expression in |
AI cell versus SO cells but not EAE sacs |
Hieracium praealtum |
| buds from specific rosette positions |
were used to conduct |
(ATH1, AT4G32980) microarray-based transcriptome profiling |
Arabidopsis thaliana |
| largest block of correlated expression |
was |
high correlation between all root samples regardless of whether distal or proximal or of nitrogen treatment |
Sorghum bicolor |
| set of 2 500 genes with largest sum magnitude of loadings for first three PCs |
were identified to |
identify genes with large variation in expression across dataset |
Sorghum bicolor |
| detection of differentially expressed genes (DEGs) |
was performed between |
experiments |
Physcomitrella patens |
| transcript levels |
were calculated as |
RPKM (reads per kilobase per million reads) |
Brassica napus |
| TranSeq |
could discriminate between |
transcripts that display multiple polyadenylation sites |
|
| strict Ni hyperaccumulator species with marked variation in shoot Ni content |
would be |
alternative model to identify patterns of gene expression underlying Ni hyperaccumulation |
|
| principal component analysis (PCA) |
profiled |
expression patterns of all genes |
Diospyros kaki |
| RNA-seq analysis |
revealed that expression of |
1064 of the 41,724 expressed genes |
Medicago truncatula |
| genes |
identified as expressed in |
at least one of 16 samples |
Cicer arietinum |
| genes upregulated in stone cell transcriptomes |
includes |
80 contigs common to upregulation in stone cells of both R and S genotypes |
Picea sitchensis |
| (APC1, PC1, AT5G17480) |
separated |
R and S genotypes |
Picea sitchensis |
| (APC1, PC1, AT5G17480) and PC2 |
accounted for |
68.5% of the original variance |
Picea sitchensis |
| ROSMETER algorithm |
uses comparative approach based on |
vector correlation |
|
| Cuffdiff |
is used for |
differential expression analysis |
Arabidopsis thaliana |
| nuclear RNA |
had about |
18,100 protein-coding transcripts detected |
Oryza sativa |
| transcriptomes of root cap, meristem, and elongation zone of gravistimulated wild-type and egt2 mutant roots |
were studied by |
RNA sequencing (RNA-seq) in time-course experiment |
Hordeum vulgare |
| fluorescence-activated cell sorting (FACS) |
is used for |
early attempts to increase the resolution of transcriptomics analysis |
|
| plant protoplasts |
is problematic as biological entities to analyze |
gene expression |
|
| 409 DEGs in OXrPPR1 plants |
include |
164 upregulated and 245 downregulated genes |
Arabidopsis thaliana |
| cDNA libraries |
were sequenced using |
Illumina HiSeq 4000 system |
Oryza sativa L. ssp. Japonica |
| differential expression analyses |
is complemented by |
co-expression network analyses |
Hordeum vulgare |
| genes upregulated in stone cell transcriptomes |
includes |
164 contigs uniquely differently expressed in S genotype |
Picea sitchensis |
| RNA-seq data |
especially for lowly expressed isoforms |
challenge in obtaining reliable information |
|
| de novo transcriptome characterization strategy |
used to |
make qualitative comparisons between the cell type transcriptomes |
Hieracium praealtum |
| DEGs downregulated in OXrPPR1 |
are also differentially expressed but in opposite direction in |
OXmiR400 plants |
Arabidopsis thaliana |
| barnyard grass transcriptome |
yielded |
278,843 transcripts and 95,223 unigenes |
|
| probe selection method based on gDNA hybridization |
reduces bias in |
transcriptome data |
Rorippa amphibia; Rorippa sylvestris |
| single-cell RNA sequencing (scRNA-seq) analysis |
identified |
fer-4 root cells exhibit a distinct clustering profile compared with wild-type roots |
Arabidopsis thaliana |
| single protoplasts isolated by glass mouth pipette |
yielded |
highly noisy RNA-seq data |
|
| marker genes of UiC2 |
enriched in |
nitrogen fixation zone (FIII) |
Medicago truncatula |
| Cufflinks |
determined |
normalized expression level as FPKM |
Cicer arietinum |
| differentially expressed genes (DEGs) between WT and ms33-6038 anthers at stages 8a–9 |
are significantly larger in number than |
DEGs at stages 5–7 |
Zea mays |
| transcriptome of S stage |
stands apart from |
transcriptomes of other developmental stages |
Petunia axillaris; Petunia exserta; Petunia parodii |
| genes |
differentially expressed between |
leaf and root |
Cicer arietinum |
| RNA-seq analysis |
generated |
7.7–41.4 million raw reads for each sample |
Cucumis sativus |
| Col-0 and bri 3 root meristems |
show |
relatively few significant gene expression differences (n = 238; p < 0.01) |
Arabidopsis thaliana |
| MpCLE2-OX lines |
contain four DETFs specific to |
Mp (ANAC092, ATNAC2, ATNAC6, NAC2, NAC6, ORE1, AT5G39610) Mp (ANAC100, ATNAC5, NAC100, NAC5, AT5G61430) Mp (ATERF14, ERF14, ERF97, AT1G04370) and Mp ASLBD11 |
Marchantia polymorpha |
| cell-based approaches to study expression profiles by RNA-Seq |
need to take into account |
possible global shift in expression between samples |
|
| Variable-shared clusters associating cultivar, vintage and location |
represent |
part of grapevine transcriptome specifically involved in G×E interactions |
Vitis vinifera |
| negative binomial generalized linear model |
was used for |
differential expression analysis |
Solanum lycopersicum; Solanum pennellii |
| transcriptome assemblies generated by 454 sequencing of nine diverse wheat cultivars |
are not present in |
Brachypodium gene set |
Triticum aestivum; Brachypodium distachyon |
| early studies of single-cell RNA-seq in plants |
opened |
new paradigm in plant single-cell transcriptome analysis |
|
| snRNA-seq advantages |
come at the cost of |
capturing substantially less genes per cell when compared with scRNA-seq |
|
| single-cell transcriptome analysis |
should be used more broadly for |
various applications in plant biology |
|
| external phloem (EP) of five tissues |
has |
10,660 genes (65.2%) commonly expressed |
Cucumis sativus |
| Sphagnum spp. |
has |
published transcriptome |
Sphagnum spp. |
| 3′-end sequencing methods |
revealed that |
most Arabidopsis genes hold alternative polyadenylation sites |
Arabidopsis thaliana |
| original aim of this study |
was |
increase throughput of transcriptomics experiments |
|
| transcript levels |
were profiled in |
RNA pools from infected and non-infected samples |
Arabidopsis thaliana |
| gl8 |
was down-regulated in |
its respective mutants as compared with wild-type controls |
Zea mays |
| PCA and differential expression analyses |
revealed that same cell types from different leaf samples |
clustered together |
Arabidopsis thaliana |
| PCA and differential expression analyses |
revealed that different cell types from different leaf samples |
separately grouped |
Arabidopsis thaliana |
| plant single-cell transcriptome analysis studies |
have taken the analysis of transcriptional activity in individual cells of the Arabidopsis root to |
unprecedented resolution |
Arabidopsis thaliana |
| genes most highly expressed in root hair cells |
identified using |
RNA sequencing analysis |
Arabidopsis thaliana |
| 31 genes |
were commonly upregulated in |
continuous external phloem (EP) system from peduncle to gynophore to fruit peripheral vascular bundle (PeVB) |
Cucumis sativus |
| approximately 81% of positive regulators identified as transcription factors |
have been classified as members of |
co-expression modules associated with increased Kranz differentiation |
Zea mays |
| RNAseq-derived transcriptomes |
are from |
sperm cells |
Oryza sativa ssp. japonica |
| transcripts with low expression |
ignored |
transcript analysis |
Oryza sativa |
| 6977 transcripts |
have mean length of |
1553 bp |
Oryza sativa |
| high-throughput sequencing technologies |
have enabled identification of |
lncRNA transcripts |
|
| TomExpress platform |
provides |
browser and integrated web tools for public RNA-Seq data visualization and data mining |
|
| 167 genes |
were more highly expressed in |
infected and mock-treated coi1-t plants |
Arabidopsis thaliana |
| fungal transcripts |
represented |
1.75% of the whole reads |
Lotus japonicus; Gigaspora margarita |
| other hydration states |
were less distinct |
in gene expression patterns |
Marchantia inflexa |
| AearMEE14 |
showed approximately 9-fold increase in transcript abundance |
(EDA33, GT140, IND, IND1, AT4G00120) flower samples |
Aethionema arabicum |
| transcriptomes of the two segregating wild types (WT coi1-t and WT (AOS, CYP74A, DDE2, AT5G42650) ) |
being most related, though still distinct |
each other |
Arabidopsis thaliana |
| 119 genes |
upregulated in |
B+ fungus compared with the cured line |
Gigaspora margarita |
| 1805 genes with increased relative transcript levels in bHLH6 OV lines |
were also upregulated in WT under |
low-P conditions |
Oryza sativa |
| samples from different genotypes, tissues and hydration states |
were broadly interspersed along |
(APC1, PC1, AT5G17480) |
Marchantia inflexa |
| large-scale transcriptomic data |
derived from |
broad range of tissues and conditions |
Arabidopsis thaliana |
| transcripts with sequence similarity to known transposable elements |
ignored |
transcript analysis |
Oryza sativa |
| S. foetida EST sequences |
contains |
21,362 seed EST assemblies |
Steculia foetida |
| 787 additional loci with sequence similarity to genomes of other species |
could correspond to |
unannotated noncoding transcripts |
Glycine max |
| transcriptome deep sequencing (RNA-seq) |
is conducted using |
2-week-old seedlings of OXmiR400 and OXrPPR1 transgenic plants and WT seedlings |
Arabidopsis thaliana |
| microarray analysis |
revealed |
novel npcRNA candidates |
yeast; plants; Homo sapiens |
| 5,315 non-diurnal genes |
showed |
lower expression levels than diurnally expressed genes |
Skeletonema robusta |
| long read technology |
enables capture of |
all the full length transcripts including splice variants |
|
| Venn diagrams |
showed the overlap for |
significantly differentially expressed transcript responses for (AOX1A, ATAOX1A, AtHSR3, HSR3, AT3G22370) and rpoTmp plants compared with wild-type (Col-0) plants |
Arabidopsis thaliana |
| genes for these transcripts |
did not overlap with the responses in |
the (AOX1A, ATAOX1A, AtHSR3, HSR3, AT3G22370) plants |
Arabidopsis thaliana |
| NME samples |
cluster closer to |
CAP samples |
Lepidium sativum |
| internal phloem (IP) of peduncle vascular bundle and peripheral vascular bundle (PeVB) |
has |
64 common upregulated genes (URGs) |
Cucumis sativus |
| Modulated genes |
were assessed for differential expression between |
2011 and 2012 vintages |
Vitis vinifera |
| 4724 Unigenes |
varied more than two-fold between |
the HS and NTSR populations |
Alopecurus myosuroides |
| RNA-seq |
was used in |
global expression profiling of soybean genes in trifoliate leaves of GmGBP1-i-4 plants compared with those in wild-type plants in (SDS, AT1G14750) at flowering induction stages |
Glycine max |
| tomato genes |
displayed similar pattern of expression in |
both TruSeq and TranSeq sequencing methods |
Solanum lycopersicum |
| maternal class (q = 1) |
represented |
13.8% of genes at globular stage |
Arabidopsis thaliana |
| inexpensive methods for profiling of the complete nuclear transcriptome |
developed for |
profiling of the complete nuclear transcriptome |
Oryza sativa L. |
| difference between these readouts |
was evident from |
weak positive correlation in abundance of RNAs in the two populations (R 0.298, Pearson correlation) |
Oryza sativa |
| 50 unique gene models |
were identified with higher differential expression in |
AI cell relative to at least one of the other cell types in both mutants |
Hieracium praealtum |
| laser capture microdissection (LCM) |
in combination with |
454 pyrosequencing, bioinformatic analyses, and in situ hybridization |
Hieracium praealtum |
| All comparisons to determine changes in transcript abundances under normal conditions |
were with |
the wild type (Col-0) |
Arabidopsis thaliana |
| high-resolution comparison of nuclear and steady-state poly(A) + transcript populations |
exposed distinctions in diversity and abundance of |
nuclear and total transcriptomes |
Oryza sativa L. |
| Total RNA-seq libraries |
were constructed with |
oligo dT-selected RNA |
Oryza sativa |
| overall transcriptome profiles |
were similar between |
the two host species |
Colletotrichum orbiculare |
| changes in transcript abundance in (AOX1A, ATAOX1A, AtHSR3, HSR3, AT3G22370) plants compared with rpoTmp plants under normal conditions compared with the wild type (Col-0) |
revealed that there was little in common between |
the two mutant lines |
Arabidopsis thaliana |
| read coverage across the transcription unit |
was averaged for |
RNAs that were significantly enriched in the nuclear or poly(A) + populations |
Oryza sativa |
| cDNA library |
revealed after sequencing |
343 unique ESTs |
Medicago truncatula |
| K19624 line |
subjected to |
three independent hybridizations |
Arabidopsis thaliana |
| differentially expressed genes (DEGs) |
identified in |
Experiment I (F-NA versus F-CA) |
|
| RNAseq-derived transcriptomes |
are from |
vegetative cells |
Oryza sativa ssp. japonica |
| transcriptome atlas |
was developed with two primary goals |
to sample major plant organs at different developmental stages and to sample diversity of nitrogen states and sources |
Sorghum bicolor |
| single-cell RNA-Seq samples |
were excluded from |
analysis |
Arabidopsis thaliana |
| Genome browser views |
illustrate |
average transcript read distribution for BETA-GALACTOSIDASE2 (BGAL2, AT3G52840) and TRANSCRIPTION FACTOR (AtWRKY68, WRKY68, AT3G62340) |
Oryza sativa |
| M. truncatula lincRNAs |
were available for analysis with |
5,794 loci |
Medicago truncatula |
| many genes in each QTL interval |
showed |
remarkable differences in expression fold difference |
Zea mays |
| RNA-seq analyses |
were performed in |
sulki1-8, Ler/ (ATKAS2, FAB1, KAS2, AT1G74960) NIL, and plants grown at 14°C to 16°C |
Arabidopsis thaliana |
| 13 gene models |
had higher expression in |
AI cell versus EAE sacs but not SO cells |
Hieracium praealtum |
| filtered reads |
had mapping rate of |
81.7% to 85.4% |
Triticum aestivum |
| untreated and MLD-treated wild-type microarrays |
still grouped closely together |
in principal component analysis |
Arabidopsis thaliana |
| relative expression of each gene in lower internodes 4 and 5 |
averaged |
expression levels across lower internodes |
Zea mays |
| mapping |
yielded |
average coverage of 26.7 (total [poly(A) + ] RNA) and 1.52 million (nuclear RNA) |
Oryza sativa |
| nuclear 5′-end bias |
was less prominent in |
transcripts with the highest nuclear abundance |
Oryza sativa |
| mRNA libraries |
were sequenced using |
Illumina technology |
Arabidopsis thaliana |
| genes with transcripts abundance that was significantly differentially expressed |
were identified with criteria |
P < 0.05; posterior probability of differential expression (PPDE) > 0.95; fold change > 1.5-fold |
Arabidopsis thaliana |
| antagonistic changes in transcript abundance between (AOX1A, ATAOX1A, AtHSR3, HSR3, AT3G22370) and rpoTmp |
were more than similarities under normal growth conditions |
between the two mutant lines |
Arabidopsis thaliana |
| A systematic comparison |
was made of |
nuclear RNA and poly(A) + RNA from root tips of seedlings |
Oryza sativa |
| GSEA |
is |
widely used transcriptome data interpretation tool |
Arabidopsis thaliana |
| NME samples at 13 h |
cluster closer to |
CAP samples |
Lepidium sativum |
| nuclear RNA-seq libraries |
were generated by |
rRNA subtraction and random-primer-enabled cDNA synthesis |
Oryza sativa |
| proportion of intronic to exonic reads (coding regions, 5′ UTR, 3′ UTR) |
was 2-fold higher in |
nuclear RNA in all of the biological replicates |
Oryza sativa |
| threshold of >5 reads per kilobase per million reads (rpkM) on exonic regions |
was applied to estimate |
complexity (i.e. the total number of gene transcripts detected) |
Oryza sativa |
| MapMan |
is |
widely used transcriptome data interpretation tool |
Arabidopsis thaliana |
| microarray analysis |
uses |
RNA samples |
Arabidopsis thaliana |
| NME samples |
cluster apart from |
CAP samples |
Lepidium sativum |
| in silico approaches |
allowed the identification of |
aposporous initial (AI)-enriched genes |
Hieracium praealtum |
| two conditions |
showed clear variation between |
expression profiles |
Triticum aestivum |
| number of DEGs at each time point |
is similar between |
Koshihikari and Takanari |
Oryza sativa |
| rice cultures exposed to cyprosulfamide |
had transcriptome changes determined by |
RNA-seq analysis |
|
| RNA-seq approaches |
were initially used in |
Chlamydomonas reinhardtii |
Chlamydomonas reinhardtii |
| nuclear RNA library sequencing |
identified |
over 7000 lncRNAs |
Oryza sativa |
| 80% reads |
were mapped to |
current soybean reference genome assembly |
Glycine max |
| TranSeq experiment |
requires |
as little as 1–2 million reads per sample to cover majority of tomato transcriptome |
Solanum lycopersicum |
| 1064 genes with significantly altered expression |
was significantly altered in |
(EMA1, GIR1, SAD2, URM9, AT2G31660) OE lines |
Medicago truncatula |
| Arabidopsis thaliana roots |
were sampled for |
RNA sequencing analysis |
Arabidopsis thaliana |
| principal component analysis |
was performed on |
global structure of the transcriptome dataset |
Arabidopsis thaliana |
| 4482 flower DEGs |
were regulated at least |
2-fold between DEH and (EDA33, GT140, IND, IND1, AT4G00120) |
Aethionema arabicum |
| Gene Ontology (GO) terms |
associated with |
up- and downregulated transcripts |
Aethionema arabicum |
| deep RNA-sequencing (RNA-seq) |
performed on |
B+ Myc and B− Myc Lotus japonicus roots |
Lotus japonicus |
| snRNA-seq |
has broader applicability to plant tissues compared to |
scRNA-seq |
|
| 14,264 transcripts |
were shared by |
both RNA populations |
Oryza sativa |
| 14 gene models |
had higher expression in |
AI cell versus both SO cells and EAE sacs |
Hieracium praealtum |
| 75 stage-specific clusters |
comprised |
6,793 genes accounting for 37.5% of all modulated genes |
Vitis vinifera |
| Vintage and location variables |
were associated with |
39 clusters and 1,478 genes |
Vitis vinifera |
| PCA |
revealed striking difference in |
gene expression clusters between Pantoea deleyi SH-355-colonization and control groups |
|
| methodology |
opens way to analyze |
transcriptomic data from tissues dissected from tomato fruit |
Solanum lycopersicum |
| high-throughput RNA sequencing (RNA-seq) |
is method of choice to define and analyze |
transcriptomes |
|
| Loci homozygous in Cabernet Sauvignon and heterozygous in Sangiovese |
were overrepresented in |
clusters of transcripts explaining G×E interactions |
Vitis vinifera |
| expression correlation in AADD allotetraploid |
was first clustered by |
subgenome (AlloA and AlloD) |
|
| TranSeq method |
detected |
17,854 genes |
Solanum lycopersicum |
| TranSeq reads originating from 3′-ends of transcripts |
is probably due to |
increased sensitivity for recent duplicates |
Solanum lycopersicum |
| wild-type (WT) Col-0 and (AT-PHH1, ATCRY2, CRY2, FHA, PHH1, AT1G04400) null-mutant seedlings |
were compared by |
RNA sequencing (RNA-seq) |
Arabidopsis thaliana |
| deep-sequencing studies |
have been conducted on |
gamete transcriptomes |
|
| TomExpress |
is fully dedicated to processing |
tomato RNA-Seq data |
Solanum lycopersicum |
| difference |
was due to |
both distinctions in transcriptome complexity and transcript abundance |
Oryza sativa |
| genes with significantly increased fold change in AI cell relative to EAE sacs and SO cell type transcriptomes |
were compared with |
differential gene lists from comparisons between whole ovary transcriptomes of apomict R35 and apospory-deficient mutants m115 and m134 |
Hieracium praealtum |
| RNA-seq reads |
mapped to |
chickpea genome (kabuli, v1.0) |
Cicer arietinum |
| 9,821 genes |
accounts for |
42.2% of annotated genes in cucumber genome |
Cucumis sativus |
| poly(A)− RNA library sequencing |
identified |
over 7000 lncRNAs |
Oryza sativa |
| cDNA libraries from Peldon and Rothamsted |
subjected to |
global RNA-Seq analysis using the IonTorrent sequencing platform |
Alopecurus myosuroides |
| expressed secreted proteins (SPs) |
were included with |
minimal read count of 100 for combined replicates |
Rhizophagus irregularis |
| RNA-seq |
broadened use from |
model organisms to numerous other organisms |
|
| PC3 |
captured variance between |
sampled tissues |
Solanum lycopersicum |
| TruSeq method |
could not detect |
gene expression in ORTHO000275_2 group |
Solanum lycopersicum |
| rest of gene families |
showed no significant difference in |
detection of gene expression between TranSeq and TruSeq |
Solanum lycopersicum |
| TranSeq method |
revealed that |
Solyc07g064130 was highly expressed while Solyc10g006480 and Solyc11g005670 displayed lower expression |
Solanum lycopersicum |
| novel methodologies to study gene expression diversity |
should deal with |
larger sample sets and minute amounts of RNA per sample |
|
| use of nuclear RNAs |
does not introduce major bias for |
gene expression studies |
|
| library containing 3′-end of all polyadenylated RNA molecules |
includes |
micro-RNAs (miRNA) |
|
| altered pattern of transcriptome in the root |
is remarkably different from |
root hair |
Zea mays |
| next-generation sequencing |
becomes challenging to obtain reliable information on spliced isoforms from |
RNA-Seq data alone |
|
| differentially expressed genes between stone cells and cortical parenchyma |
revealed |
244 transcripts significantly upregulated in stone cells |
Picea sitchensis |
| gDNA hybridization signal threshold of 80 |
retains |
97% of probe sets |
Rorippa amphibia; Rorippa sylvestris |
| 220 putative effectors predicted by Sędzielewska Toro and Brachmann |
could only confirm |
43 secreted proteins (SPs) |
Rhizophagus irregularis |
| CR-lncRNA1459-21-1 mutant |
has |
3379 upregulated DEGs and 1584 downregulated DEGs |
Solanum lycopersicum |
| TruSeq method |
was more sensitive and outcompeted |
TranSeq in absolute numbers of detected duplicates |
Solanum lycopersicum |
| marker genes of UiC1 and UiC2 |
enriched in |
un-infected cells localized in fixation zone |
Medicago truncatula |
| late ABA-responsive factor (factor 5) |
is present in |
cluster 5 |
Talinum triangulare |
| gene ontology (GO) terms related to meristem development, cell growth, anatomical structure morphogenesis/development, macromolecule or primary or secondary metabolic process, defense response and trichoblast differentiation |
were highly enriched in |
upregulated genes (URGs) in peripheral vascular bundle (PeVB) |
Cucumis sativus |
| Bryum argenteum |
has |
published transcriptome |
Bryum argenteum |
| Pellia endiviifolia |
has |
published transcriptome dataset |
Pellia endiviifolia |
| outer cotyledon (OC) and inner cotyledon (IC) |
could not be separated in |
principal component analysis |
Brassica napus |
| TranSeq |
generates reads matching |
3′UTRs of transcripts |
|
| top 50 marker genes from each cluster |
screened in |
different tissues/zones of 15-dpi and 21-dpi nodules |
Medicago truncatula |
| 3478 genes |
were |
highly expressed under –Fe conditions |
Triticum aestivum |