| rise in 3-phosphoglycerate (3PGA) and fall in orthophosphate (Pi) |
strongly activate |
ADP-glucose pyrophosphorylase (AGPase) |
higher plants |
| induced TPS plants |
showed transient increase in rate of starch accumulation from 4 to 6 h after induction |
starch accumulation rate |
Arabidopsis thaliana |
| (APL3, AT4G39210) gene |
encodes |
one of the large subunits of ADP-glucose pyrophosphorylase (AGPase) |
Arabidopsis thaliana |
| pyruvate |
is recycled to |
stored starch |
|
| three SBEs |
was decreased gradually |
amylopectin synthesis |
|
| gradual reduction of SBEs from TRS polygonal to hollow granules |
decreased |
amylopectin synthesis |
|
| amyloplasts |
are |
specialised nongreen plastids |
|
| ADP-Glc levels in GlgC-TM lines C3 and C4 at night |
were much higher than |
wild-type plants |
Arabidopsis thaliana |
| GlgC-TM plants |
were unable to adjust |
the rate of starch synthesis in response to daylength |
Arabidopsis thaliana |
| TRs |
shows amylose content per grain increased by |
40% |
|
| trimeric assembly of SSI, SSIIa, and SBEIIb in the stroma of amyloplasts |
occurs as in |
wild type |
|
| allocations of granule-bound amylopectin biosynthetic enzymes among four TRS heterogeneous granules |
were investigated to disclose |
amylopectin difference among four TRS heterogeneous granules |
|
| trehalose |
induced |
expression of the (APL3, AT4G39210) gene |
Arabidopsis thaliana |
| redox status of ADP-glucose pyrophosphorylase (AGPase) after TPS induction |
showed no reproducible differences between |
induced and noninduced plants |
Arabidopsis thaliana |
| ISOAMYLASE1-ISOAMYLASE2 (ISA) mutants |
alter |
branching pattern |
Arabidopsis thaliana |
| accumulation of sucrose |
stimulates |
starch synthesis via activation of adenosine 5′-diphosphate-glucose pyrophosphorylase (AGPase) |
Arabidopsis thaliana |
| starch contents at the end of the day in GlgC-TM and (ADG1, APS1, AT5G48300) lines |
were close to |
those of wild-type plants |
Arabidopsis thaliana |
| transgenic plants containing a redox-insensitive AGPase |
showed starch synthesis acceleration following |
an unexpected extension of the night to almost the same extent as in wild-type plants |
Arabidopsis thaliana |
| SBEs in TRS hollow starch |
were seriously inhibited |
|
|
| redox modulation of enzyme activity |
is not essential for |
adjustment of starch synthesis to daylength |
Arabidopsis thaliana |
| starch content elevation |
could occur even though |
amounts and activity of starch synthase including GBSSI were not increased |
|
| amount of SBEIIb in starch granules |
was correlated significantly and positively with |
amylopectin content |
|
| enzyme proteins |
are prone to forming |
varieties of complexes in the soluble amyloplast fraction |
|
| rate of starch synthesis |
is determined by |
rate of net CO2 fixation and partitioning of photoassimilates between starch and sucrose |
Arabidopsis thaliana |
| (AtCCA1, CCA1, AT2G46830) (LHY, LHY1, AT1G01060) mutants |
accumulated less starch than |
wild-type plants |
Arabidopsis thaliana |
| more than seven enzyme proteins |
are responsible for |
amylopectin formation |
|
| rate of starch synthesis in most GlgC-TM and (ADG1, APS1, AT5G48300) lines |
was comparable with |
wild type in 12:12 conditions |
Arabidopsis thaliana |
| level of ADP-Glc at night |
is a measure of |
the rate of starch synthesis |
Arabidopsis thaliana |
| AGPase activity in extracts of GlgC-TM plants |
is greatly in excess of |
observed rates of starch synthesis |
Arabidopsis thaliana |
| stromules |
are site of initiation of |
small starch granules |
developing wheat endosperm |
| ss2ss3 plants |
were almost devoid of |
glucan |
Arabidopsis thaliana |
| GlgC-TM lines C3 and C4 |
had AGPase activity higher than |
wild-type plants under both photoperiods |
Arabidopsis thaliana |
| rate of starch synthesis in wild-type plants |
was doubled in |
short days |
Arabidopsis thaliana |
| nitrate |
represses expression of |
AGPs |
Arabidopsis thaliana |
| starch phosphorylase 1 (Pho1) |
plays a role in |
starch synthesis |
|
| double helices |
are packed laterally to form |
crystalline lamellae |
|
| amount of SBEIIb in starch granules |
was correlated significantly and positively with |
branching degree of amylopectin |
|
| glutelin GT1 (GluA-2) promoter in TRS transgenic plants |
might be responsible for |
regional distribution of the three SBEs in a single seed |
|
| regulatory properties of the native AGPase |
are essential for |
adjustment of the rate of starch synthesis to daylength |
Arabidopsis thaliana |
| transgenic plants containing a redox-insensitive AGPase |
behave like wild-type plants in that starch synthesis is accelerated in response to |
growth in short days |
Arabidopsis thaliana |
| (ADG2, APL1, AT5G19220) mutant |
showed acceleration of starch synthesis following an unexpected extension of the night of only about one-half that in |
wild-type plants |
Arabidopsis thaliana |
| (AtSS2, SS2, AT3G01180) mutation |
decreased |
starch synthesis |
Arabidopsis thaliana |
| allosteric properties of the native enzyme |
are important for maintaining |
appropriate concentration of ADP-Glc for starch synthesis during the day |
Arabidopsis thaliana |
| SBE inhibition in TRS hollow starch |
leads to |
amylopectin branch-chain length increasing from DP 22.8 to DP 37.6 |
|
| amount of SBEIIa in starch granules |
was correlated negatively with |
amylose content |
|
| ADP-Glc levels in GlgC-TM lines |
were lower during the day and substantially higher at night in |
wild-type plants |
Arabidopsis thaliana |
| Amylopectin branch chains |
form |
double helices |
|
| decreased amylopectin in TRS hollow starch |
increased |
true amylose content from about 17% to 75% |
|
| granule-bound amylopectin biosynthetic enzymes |
can be used as |
important indicator of enzyme action on the formation of amylopectin molecular structure |
|
| rate of starch synthesis in GlgC-TM plants |
was unaffected or reduced in |
short days |
Arabidopsis thaliana |
| Amylopectin synthesis per grain in TRS |
decreased by |
70% |
|
| two maize ae mutations |
lead to |
absence of SBEIIb in ae − (ae1.1) and a catalytically inactive form of SBEIIb in ae1.2 |
|
| increase in AGPase activity |
may be important to support |
higher flux to starch in roots |
|
| (ADG1, APS1, AT5G48300) line N2 |
had AGPase activity similar to |
wild-type plants under both 12:12 and 6:18 growth conditions |
Arabidopsis thaliana |
| accelerated sucrose synthesis |
will result in |
low rates of starch synthesis via allosteric inhibition of AGPase |
Arabidopsis thaliana |
| allosteric regulation |
makes a much larger contribution to prevention of starch synthesis at night than |
redox inactivation |
Arabidopsis thaliana |
| ss2ss3 plants |
contained |
phytoglycogen |
Arabidopsis thaliana |
| GlgC-TM plants |
showed no acceleration of starch synthesis following |
an extended night |
Arabidopsis thaliana |
| failure of (ADO3, FKF1, AT1G68050) and gi mutants to adjust the rate of starch synthesis to daylength |
may reflect |
altered diel patterns of growth in these mutants |
Arabidopsis thaliana |
| (AtDPE1, DPE1, AT5G64860) (MEX1, RCP1, AT5G17520) double mutant |
is capable of synthesizing |
starch |
Arabidopsis thaliana |
| isoamylase-type debranching enzyme |
is thought to facilitate |
formation of crystalline amylopectin layers |
|
| amount of SBEI in starch granules |
was correlated significantly and positively with |
amylopectin content |
|
| amount of SBEI in starch granules |
was correlated significantly and positively with |
branching degree of amylopectin |
|
| (AtCCA1, CCA1, AT2G46830) (LHY, LHY1, AT1G01060) mutants |
showed higher rate of starch accumulation in short photoperiods compared with |
long photoperiods |
Arabidopsis thaliana |
| starch synthesis |
is catalyzed by |
isoamylase-type debranching enzymes |
|
| three SBEs reduction by more than 70% in barley |
results in |
amylopectin synthesis completely inhibited |
Hordeum vulgare |
| amount of SBEI in starch granules |
was correlated negatively with |
amylopectin branch chain length |
|
| SSIIIa in the granule-bound fraction in both TQ and TRS |
was not detected |
|
|
| ADP-Glc in adg1-1 plants |
was almost undetectable |
under all conditions |
Arabidopsis thaliana |
| (AtSS2, SS2, AT3G01180) |
is important for |
establishing proper amylopectin structure |
|
| enriched ADP-Glc supply |
could elevate |
starch content |
|
| amount of SBEIIb in starch granules |
was correlated negatively with |
amylose content |
|
| gradual reduction of SBEs from TRS polygonal to hollow granules |
changed |
molecular structure of amylopectin |
|
| complex in su2 mutant |
causes inability to become |
granule-bound component |
|
| SSI function in generating chains of DP 8-12 |
greatly helped to elucidate |
gradually decreasing ratio of DP 6-12 in the four TRS heterogeneous starch granules |
|
| SSIIa |
traffics other enzymes into |
granule |
|
| starch |
is synthesized in |
chloroplasts |
|
| (ATSS4, SS4, SSIV, AT4G18240) |
does not have major influence on |
amylose synthesis |
|
| amylose content of starch |
potentially correlates with |
availability of ADP-Glc |
|
| (ELF3, PYK20, AT2G25930) mutants |
accumulated less starch than |
wild-type plants |
Arabidopsis thaliana |
| gluconeogenesis |
participates in |
starch synthesis pathway |
|
| GBSSI content in ss3a / Waxy a |
is not significantly different from |
GBSSI content in indica itself (SS3a / Waxy a) |
|
| trehalose-6-phosphate (T6P) |
controls |
rate of starch synthesis |
|
| (APL3, AT4G39210) |
encodes |
large subunit of AGPase |
Arabidopsis thaliana |
| starch synthases and branching enzymes |
elongate |
glucan chains of the starch granule |
|
| starch synthase (SS) activity in ss2ss3 plants |
is not |
limiting for glucan accumulation |
Arabidopsis thaliana |
| plants expressing deregulated AGPase in 12:12 conditions |
had end-of-day starch contents of 60% to 100% of |
wild-type values |
Arabidopsis thaliana |
| more and more space released for amylose synthesis and accumulation in lamellae |
occurs from TRS polygonal to hollow starch granules even though |
GBSSI contents were not increased |
|
| amylose interaction |
forms |
antiparallel double helices |
|
| amount of SBEIIa in starch granules |
was correlated significantly and positively with |
amylopectin content |
|
| internal granule-bound components in ae1.2 |
consist of |
SSI, SSIIa, SBEI, SBEIIa, and null SBEIIb |
|
| TRS heterogeneous starch granules |
contained gradually decreased |
amylopectin |
|
| altered amounts of three SBEs acting on the formation of four heterogeneous granules |
along with |
other proteins also effected changes, including SSI and (ATPHO1, PHO1, AT3G23430) |
|
| Glucose-6-phosphate in amyloplasts |
is used for |
subsequent starch synthesis |
|
| LATE ELONGATED HYPOCOTYL (LHY, LHY1, AT1G01060) |
regulates |
GRANULE-BOUND STARCH SYNTHASE I (GBSS1, AT1G32900) |
|
| SSIIa |
is responsible for |
granule-association of SSI and SBEIIb |
Zea mays |
| bacterial glgC gene (deregulated version of starch biosynthesis enzyme AGPase) |
was overexpressed in |
cassava storage roots |
|
| reduction of VIN activity in maize kernels |
reduced |
starch biosynthesis in the endosperm |
Zea mays |
| AGPase gene (AGPS) |
encodes |
regulatory subunit of AGPase |
|
| SSIIa phosphorylation |
affects |
interaction of SSIIa with other starch biosynthetic enzymes |
Zea mays |
| transcription factors (TFs) |
are considered as |
important regulatory factors of starch synthesis genes (SSGs) |
Zea mays |
| miRNAs |
are highly important for |
starch accumulation in cereal crops |
|
| miRNAs |
have illuminated regulatory functions in |
maize starch synthesis |
Zea mays |
| enzymatic assay of total soluble extracts |
indicates compensation for loss of |
particular SS activity |
|
| three enzymes involved in the starch synthesis pathways |
participate in |
metabolite concentration prediction |
Arabidopsis thaliana |
| high activities of enzymes in the Calvin–Benson cycle and pathways for sucrose and starch synthesis |
are likely attributed to |
high rate of photosynthesis |
Manihot esculenta |
| starch metabolism |
is controlled by |
starch synthase |
Zea mays |
| ZmMYB115 |
is co-expressed with |
starch synthesis-associated genes |
Zea mays |
| ADP-Glc (adenosine 5-diphosphate-glucose) contents of wild-type (Col-0) plants, (ADG1, APS1, AT5G48300) control line N2, and GlgC-TM lines C3 and C4 |
were measured in |
plants grown in 12:12 conditions and harvested at 3 h (ZT3) and 15 h (ZT15) after dawn |
Arabidopsis thaliana |
| pyruvate orthophosphate dikinase (PPDK, AT4G15530) |
participates in |
starch synthesis pathway |
|
| amylopectin synthesis reduction |
reduces |
steric hindrance for amylose accumulation in crystalline and amorphous lamellae |
|
| DNA methylation |
was identified as |
master switch |
Zea mays |
| multi-level transcriptional regulation atlas |
involves |
starch synthesis genes |
Zea mays |
| elevated total amylose content |
occurs only in the absence of SS2 and the presence of |
(SS3, AT1G74000) |
|
| increased (ATSS1, SS1, AT5G24300) activity in Arabidopsis ss3- mutant |
is probably not due to |
increased expression of the structural gene of (ATSS1, SS1, AT5G24300) |
Arabidopsis thaliana |
| at least two SSs |
must be missing in order to observe |
effect on branch placement |
|
| reduction and monomerization of AGPase |
result in |
activation of AGPase |
|
| glgC overexpression in cassava storage roots |
resulted in 2.6-fold higher |
storage root biomass compared with control plants |
|
| increased temperatures |
perturb |
starch synthesis |
|
| starch synthase II (SSII) |
elongates |
intermediate glucan chains (DP 12–24) |
|
| starch synthesis enzymes |
were similar in |
mesophyll and bundle sheath cells |
Oryza sativa |
| elevated glycogen synthase activity |
had effects on |
starch synthesis |
Triticum aestivum |
| SSIIa |
is subject to |
phosphorylation |
Zea mays |
| AGPS2 |
is |
Group 1 gene with up-regulated expression at 9 (DPA, AT5G02470) in superior spikelets |
Oryza sativa |
| substrate for starch synthesis in the cytosol |
was probably |
UDPglucose |
|
| starch synthases (SSs) |
synthesize |
amylose and amylopectin |
|
| SSIIa mutants |
exhibit a characteristic decrease in |
frequency of intermediate chains with DP 12–22 |
Triticum aestivum; Hordeum vulgare; Oryza sativa; Zea mays |
| SSIIa |
associates with |
SSI and SBEIIb |
Triticum aestivum; Zea mays; Hordeum vulgare; Oryza sativa |
| ADP-glucose pyrophosphorylase subunits ( (ADG2, APL1, AT5G19220) and (APS2, ASA1, ATPS2, AT1G19920) ) |
were more abundant in |
mesophyll cells |
Oryza sativa |
| depletion of ADPGlc pool by introduced glucan synthase |
could cause |
cessation of starch accumulation |
|
| starch synthesis research |
has advanced at |
rapid pace |
|
| ADP-glucose |
is used by |
starch synthases and branching enzymes |
|
| SSII |
is mostly granule bound |
starch granules |
Pisum sativum; Arabidopsis thaliana |
| miRNA-targeted transcription factors |
are co-expressed with |
starch synthesis-associated genes |
Zea mays |
| starch synthesis genes (SSGs) |
expression is regulated by |
transcription factors (TFs) |
|
| starch synthesis mutants |
show strongly impaired |
growth |
|
| products of photosynthesis |
are usually imported into |
developing storage organ |
|
| glucose 6-phosphate |
is converted to |
glucose 1-phosphate |
|
| Iso-1 |
is not present as a starch granule-associated protein in |
wild-type or ae endosperm |
Zea mays |
| debranching enzymes (DBEs) |
affects |
chloroplastidial maltodextrin pool |
Arabidopsis thaliana |
| SSI |
elongates |
shorter glucan chains with DP 8–12 |
|
| starch content in homozygous lines expressing glycogen synthase |
was up to 93% lower than in |
control lines |
Triticum aestivum |
| AGPase activity in homozygous transgenic grains |
was strongly reduced by up to 80%, relative to grains of |
bar control |
Triticum aestivum |
| glgC overexpression in cassava storage roots |
resulted in significant increases in |
above-ground biomass |
|
| regulation of root AGPase activity (sink capacity) |
is specific target trait for |
future improvement of cassava |
|
| cereal endosperm |
has |
endogenous ADPGlc pool entirely committed to storage carbohydrate synthesis |
|
| effect of transgenes on starch content at maturity |
was equally strong in grains that developed on |
T3 homozygous plants |
Triticum aestivum |
| high amylose content |
has been proposed to cause |
abnormal granule shapes |
|
| SBE 2 and SBE 3 |
have redundant activities in |
starch synthesis |
Arabidopsis thaliana |
| BEs |
require glucans of sufficient length in order to create branches through |
intra- or intermolecular rearrangement |
|
| starch synthesis |
is |
essential feature of crop filling |
|
| transcription factors (TFs) |
have been identified as |
regulators of starch synthesis genes (SSGs) in maize endosperm |
Zea mays |
| ZmMYB115 |
was experimentally verified for regulation of |
starch synthesis genes |
Zea mays |
| soluble starch synthase 2 (AtSS2, SS2, AT3G01180) |
is |
one of three soluble starch synthases |
|
| appropriate concentration of abscisic acid (ABA) |
can enhance |
sucrose synthase (SUS) activity |
Oryza sativa |
| glucose 6-phosphate and ATP |
are imported from |
cytosol |
|
| SBEIIb |
is detected in |
normal maize amyloplast lysates |
Zea mays |
| SSI |
co-precipitates with |
SSIIa |
Zea mays |
| bundle sheath cells of C4 plants |
are the primary location of |
starch synthesis |
|
| inorganic phosphate (Pi) |
has a direct influence on |
starch synthesis |
|
| remaining SS |
is more active when SS1 is absent |
SS activity in ss1- ss2- double mutant |
|
| activity of (ATSS1, SS1, AT5G24300) |
decreases rapidly with increasing DP of the substrate, to virtually none at |
DP 12 |
|
| modified amylopectin structure |
could alter |
(GBSS1, AT1G32900) activity by offering a different glucan substrate for elongation |
|
| SSIII |
produces |
relatively longer glucan chains (DP 25–40) |
|
| DNA methylation |
has illuminated regulatory functions in |
maize starch synthesis |
Zea mays |
| these glucans |
are too short to serve as substrates for |
BEs |
|
| free carbohydrate |
is converted into |
starch |
|
| (GBSS1, AT1G32900) protein abundance |
can be ruled out as an explanation |
altered (GBSS1, AT1G32900) activity in ss2- and ss1- ss2- mutants |
|
| plastid-localized ADP-glucose production |
is not |
only pathway of starch synthesis |
|
| crystalline matrix necessary to support (GBSS1, AT1G32900) activity |
is unlikely to form |
multiple mutant combinations lacking (SS3, AT1G74000) |
|
| (ATSS1, SS1, AT5G24300) |
appears to provide more activity than normal in extracts lacking |
(AtSS2, SS2, AT3G01180) or (SS3, AT1G74000) |
|
| fine structure of amylopectin |
determines |
granule morphology through higher order structural assembly |
|
| SBE II lesion in pea embryos |
yields |
rugosous wrinkled seed phenotype |
Pisum sativum |
| either SBE 2 or SBE 3 isoform |
is sufficient for |
normal starch synthesis |
Arabidopsis thaliana |
| (AtSS2, SS2, AT3G01180) and (SS3, AT1G74000) |
both function as negative regulators of |
(ATSS1, SS1, AT5G24300) activity |
|
| hydrolysis of some branch linkages after they have been placed by a BE |
is necessary to achieve |
normal amylopectin structure |
|
| potential substrates of BE |
could be altered as the result of |
reduced SS activity |
|
| (ATSUS4, SUS4, AT3G43190) |
is |
Group 1 gene with up-regulated expression at 9 (DPA, AT5G02470) in superior spikelets |
Oryza sativa |
| higher ABA level at early stage of development of caryopses or in inferior spikelets |
suppresses |
Sus expression |
Oryza sativa |
| granule size and shape determination |
is |
poorly characterized |
|
| cytosolic form of ADPglucose pyrophosphorylase (AGPase) |
is localized to |
cytosol |
|
| total branching enzyme activities |
is approximately half in |
ae mutant amyloplasts |
Zea mays |
| soluble starch synthase (SSS) |
is involved in |
amylopectin synthesis |
Oryza sativa |
| cultivar differences in starch concentrations |
were not observed under |
growth conditions in planta or on detached panicles |
Avena sativa |
| ADPglucose |
is |
precursor for starch synthesis |
|
| interconnections between various activities that build amylopectin |
determine |
molecular architecture of amylopectin and crystalline starch |
|
| structural flaws |
amplify during |
granule expansion |
|
| relatively high concentrations of abscisic acid (ABA) in inferior spikelets |
suppress |
expression of starch synthesis genes |
Oryza sativa |
| BT1-2 |
is |
Group 1 gene with up-regulated expression at 9 (DPA, AT5G02470) in superior spikelets |
Oryza sativa |
| fluridone |
increased expression of |
(ATSUS4, SUS4, AT3G43190) |
Oryza sativa |
| amount of labelled starch between 4 h and 8 h |
increased more quickly at 12/8 °C |
higher temperature regime of 18/14 °C |
Crocus vernus |
| SSI |
co-precipitates with |
SBEIIb |
Zea mays |
| SSIIa |
interacts with |
SSI and SBEIIb |
Zea mays |
| starch metabolism |
is controlled by |
starch branching enzyme |
Zea mays |
| expression of genes coding for starch-synthesizing enzymes |
is often reduced by |
methylation |
Oryza sativa |
| blockage of Golgi secretion |
stimulates |
starch synthesis |
Nicotiana tabacum |
| chromoplasts |
contain |
proteins involved in starch synthesis |
Solanum lycopersicum |
| starch synthesis |
occurs in |
chloroplasts |
|
| ZmMYB138 |
was experimentally verified for regulation of |
starch synthesis genes |
Zea mays |
| SPK knockout |
resulted in |
chalky seeds |
Oryza sativa |
| starch synthesis |
is |
pathway |
|
| chloroplast isoenzyme of phosphoglucose isomerase (PGI, PGI1, AT4G24620) |
exerts control over |
rate of starch synthesis at saturating light intensity and CO2 |
|
| at least two SSs |
are required for |
normal architecture |
|
| chloroplast isoenzyme of phosphoglucose isomerase (PGI, PGI1, AT4G24620) |
does not exert control over |
rate of starch synthesis at low light intensity |
|
| differences in apparent affinity for ADP-glucose substrate |
exist between |
GBSS and other SSs |
|
| ss2- ss3- double mutants or ss1- ss2- ss3- triple mutant |
have total starch level drastically reduced including a large decrease in |
total amylose content in leaves |
|
| starch structure |
is altered in |
any single or double mutant line |
|
| SSIIa |
is |
Group 1 gene with up-regulated expression at 9 (DPA, AT5G02470) in superior spikelets |
Oryza sativa |
| Group 2 genes |
were presumed to be involved in |
construction of fundamental cell machinery and initiation of starch granules |
Oryza sativa |
| 60 kDa band |
is thought to be |
granule-bound starch synthase (GBSS) |
Oryza sativa |
| high concentration of abscisic acid (ABA) |
lowers |
ability of grains to synthesize starch |
Oryza sativa |
| largest fold changes between superior and inferior grains |
were in the genes responsible for conversion of |
sucrose into ADP-glucose |
Oryza sativa |
| phosphoenolpyruvate (PEP) or pyruvate produced during malate decarboxylation in the light |
must be imported into |
chloroplast |
|
| glucose 6-phosphate and ATP |
are imported into |
plastids |
|
| exogenously applied trehalose |
induces |
AGPase gene |
Arabidopsis thaliana |
| sugar accumulation in (ATPGMP, PGM, PGM1, STF1, AT5G51820) light |
is a direct consequence of |
lesion in starch synthesis |
|
| ADPglucose pyrophosphorylase (AGPase) |
catalyzes synthesis of |
ADPglucose |
|
| SSI and SSIIa |
form a complex with |
SBEIIb |
Zea mays |
| ethephon |
suppressed |
SSSIIa |
Oryza sativa |
| two genes encoding ADP-glucose pyrophosphorylase (AGPase) and one encoding a glucose 6P/P translocator |
induced at |
days 6 and 10 (AOS, CYP74A, DDE2, AT5G42650) |
Zea mays |
| sucrose synthase (SUS) |
is involved in |
pathway of starch synthesis |
Oryza sativa |
| AGPS1 |
is |
Group 1 gene with up-regulated expression at 9 (DPA, AT5G02470) in superior spikelets |
Oryza sativa |
| cytosolic ADPglucose |
is imported into |
plastids |
|
| starch synthesis pathway in grass endosperm |
has features that are unique to |
cereal endosperms |
|
| products of photosynthesis |
are imported from |
leaves |
|
| soluble starch synthase activity |
is significantly higher in |
ae mutant amyloplasts |
Zea mays |
| recombinant maize SBEI |
interacts with |
SBEIIb |
Zea mays |
| granule-bound starch synthase (GBSS) |
is involved in |
amylose synthesis |
Oryza sativa |
| SSI, SSIIa, SBEI, SBEIIa, plastidial SP, Iso-1, Iso-2, and GBSSI |
are present at comparable levels in |
ae mutant amyloplast lysates |
Zea mays |
| SBEIIb |
shows no interaction with |
SP |
Zea mays |
| 110 kDa polypeptide |
cross-reacts with |
anti-maize SP antibodies |
Zea mays |
| starch synthesis |
is |
important process during late seed development |
Oryza sativa |
| exogenous application of ABA to detached rice ears at 9 (DPA, AT5G02470) |
had little effect on |
genes and enzymes responsible for conversion of ADP-glucose to starch |
Oryza sativa |
| ADPglucose pyrophosphorylase (AGPase) |
uses substrates |
glucose 1-phosphate and ATP |
|
| functional redundancy |
is demonstrated by |
previous work in potato and Arabidopsis |
Solanum tuberosum; Arabidopsis thaliana |
| relatively high concentrations of ethylene in inferior spikelets |
suppress |
expression of starch synthesis genes |
Oryza sativa |
| AGPL2 expression |
decreased by ethephon |
61.0% |
Oryza sativa |
| fluridone |
slightly enhanced the activity of |
SSs |
Oryza sativa |
| starch synthesis |
occurs in |
amyloplasts |
|
| SSI and SSIIa |
elute earlier from column in |
ae mutant amyloplast extracts |
Zea mays |
| SSI and SSIIa |
are components of larger complexes in |
ae mutant amyloplasts |
Zea mays |
| recombinant maize SBEIIa |
interacts with |
SSI, SSIIa, SBEI, and SP |
Zea mays |
| AGPase |
is involved in |
pathway of starch synthesis |
Oryza sativa |
| triose phosphates |
can be stored as |
transitory starch |
|
| starch granule synthesis |
requires |
isoamylase and other enzymes |
|
| relatively high concentrations of ethylene and ABA in inferior spikelets |
suppress |
starch synthesis genes enzyme activities |
Oryza sativa |
| SP activity |
is reduced in |
ae mutant amyloplasts |
Zea mays |
| SSIIa |
is present at significantly higher levels in |
ae starch granules |
Zea mays |
| amylose levels |
cannot be elevated in |
ss1- mutants |
|
| compensation of SS activity in the different mutants |
should be considered as compensating mechanism between |
(ATSS1, SS1, AT5G24300) and (SS3, AT1G74000) only |
|
| amylose and amylopectin |
are branched by |
starch-branching enzyme isoforms |
|
| protein–protein interactions among starch synthesizing enzymes |
are observed at |
20–25 DAP and 29–35 DAP |
Zea mays |
| exogenous application of ABA to detached rice ears at 9 (DPA, AT5G02470) |
decreased both the expression profile of |
SUS genes |
Oryza sativa |
| SSI and SSIIa complex |
has approximate molecular mass of |
300 kDa |
Zea mays |
| (ATSUS2, SSA, SUS2, AT5G49190) |
is |
Group 1 gene with up-regulated expression at 9 (DPA, AT5G02470) in superior spikelets |
Oryza sativa |
| BEIIb |
is |
Group 1 gene with up-regulated expression at 9 (DPA, AT5G02470) in superior spikelets |
Oryza sativa |
| starch |
is synthesized from |
products of photosynthesis |
|
| ADP-glucose pyrophosphorylases |
synthesizes |
ADP-glucose |
|
| starch-binding CBM motifs |
are conserved |
starch biosynthesis enzymes |
|
| plastidial pathway of starch synthesis |
is found in |
all extant higher plants and green algae |
|
| SBEIIb |
is absent in |
ae mutant endosperm |
Zea mays |
| induced and control plants |
contained very similar levels of |
ADP-glucose (ADPG) |
Arabidopsis thaliana |
| amyloplasts |
synthesise |
starch |
|
| sacred lotus |
has significant expansion of |
starch-related genes (GBSS genes) |
|
| crude extract of soluble starch synthase from wheat endosperm |
shows a decrease in enzyme activity even at 25 °C with a 2h treatment |
enzyme activity |
Triticum aestivum |
| AGPase expression |
was down-regulated in |
SlAREB1 overexpression line at immature green stage |
Solanum lycopersicum |
| (ASUS1, atsus1, SUS1, SUSY1, AT5G20830) |
is |
Group 2 gene with down-regulated expression at 9 (DPA, AT5G02470) in superior spikelets |
Oryza sativa |
| PGIb |
is |
Group 3 gene with little changed expression during grain filling |
Oryza sativa |
| BEIIa |
is |
Group 3 gene with little changed expression during grain filling |
Oryza sativa |
| ADPglucose |
continued to be used for |
starch synthesis |
|
| SBEI |
co-precipitates with |
SSI and SSIIa |
Zea mays |
| 110 kDa polypeptide |
is present exclusively in |
ae starch granules |
Zea mays |
| manipulating starch synthesis |
could improve |
crop yield |
|
| illumination with 300 μmol m−2 s−1 of red light |
promoted |
efficient starch synthesis in guard cells |
Arabidopsis thaliana |
| starch-branching enzyme (SBE) |
is important for |
starch storage |
|
| PGIa |
is |
Group 3 gene with little changed expression during grain filling |
Oryza sativa |
| ADPglucose |
was synthesized by |
AGPase in free-living cyanobacterium |
|
| ADPglucose |
has continued to be synthesized in |
symbiont/plastid |
|
| SBEI |
has reduced catalytic activity in |
ae mutant amyloplasts |
Zea mays |
| localization of starch storage in BSCs |
probably requires |
higher rates of starch synthesis to sequester triose-phosphates efficiently |
Miscanthus maximus |
| crude extract of soluble starch synthase from wheat endosperm |
loses its enzyme activity significantly with a 15min treatment at 30 °C |
enzyme activity |
Triticum aestivum |
| Arabidopsis thaliana leaves |
synthesize starch faster in |
short days |
Arabidopsis thaliana |
| adenosine 5-diphosphate-glucose pyrophosphorylase (AGPase)-dependent mechanism |
influences |
rate of starch synthesis |
|
| adg1-1 mutant |
has |
very little AGPase activity or starch in leaves |
Arabidopsis thaliana |
| allosteric properties of the enzyme |
may permit |
adjustment of the rate of starch synthesis to different daylengths |
Arabidopsis thaliana |
| common catalytic domain of starch synthases |
is comprised of |
glycosyltransferase 5 (GT5) and glycosyltransferase 1 (GT1) subdomains |
|
| amylopectin content and molecular structure change |
leads to |
amylose accumulated in crystalline lamellae |
|
| amount of SBEI in starch granules |
was correlated negatively with |
amylose content |
|
| amount of SBEIIb in starch granules |
was correlated significantly and positively with |
short branch chain of amylopectin |
|
| (ATPHO1, PHO1, AT3G23430) |
was absent in |
control |
|
| (ATPHO1, PHO1, AT3G23430) amounts in aggregate, elongated, and hollow starches |
were not significantly different from |
each other |
|
| expanded physical space |
might be responsible for |
different amylose accumulation in four TRS heterogeneous starch granules |
|
| transgenic lines expressing a modified large subunit that renders AGPase more sensitive to activation and less sensitive to inhibition |
showed acceleration of starch synthesis following an unexpected extension of the night of only about one-half that in |
wild-type plants |
Arabidopsis thaliana |
| accumulation of phosphorylated intermediates |
will result in |
acceleration of starch synthesis via allosteric activation of AGPase |
Arabidopsis thaliana |
| amylopectin synthesis decrease |
results in |
enlarged physical space |
|
| three SBEs in TRS polygonal starches |
exhibited 28%, 51%, and 51% levels relative to |
TQ |
|
| enzyme proteins |
instead of operating individually |
|
|
| starch |
is synthesized in |
plastids |
|
| expression of either (ADG1, APS1, AT5G48300) or GlgC-TM in -1 plants |
restored |
starch synthesis in leaves |
Arabidopsis thaliana |
| granule-bound (ATPHO1, PHO1, AT3G23430) in TRS |
might facilitate |
MOS synthesis |
|
| starch |
is synthesized during |
day |
|
| soluble starch synthase |
is |
thermolabile |
Triticum aestivum |
| increased rate of starch accumulation in short photoperiods |
does not require |
adjustment of the subunit composition of AGPase |
Arabidopsis thaliana |
| immunostaining analysis of developing rice kernels |
indicates |
gradually decreasing dosages of SBEI, SBEIIa, and SBEIIb |
Oryza sativa |
| activation of ADP-glucose pyrophosphorylase (AGPase) |
increases |
production of ADP-glucose (ADPG) |
higher plants |
| induced increase in trehalose-6-phosphate (Tre6P) |
led to |
slight stimulation of starch synthesis |
Arabidopsis thaliana |
| redox activation of ADP-glucose pyrophosphorylase (AGPase) |
may require |
concerted action of two or more signaling pathways |
Arabidopsis thaliana |
| loss of LeafL function |
substantially reduces, but does not always abolish, |
heat-stable activity |
Zea mays |
| loss of genes encoding the other isoforms |
has little to no effect on |
leaf starch |
Zea mays |
| vast majority of the activity remaining in the sh2 and (ATBT2, BT2, AT3G48360) mutants |
is |
heat stable |
Zea mays |
| reduced carbon |
is converted to |
starch |
|
| ADP-Glc at night in lines containing a redox-insensitive AGPase |
was also elevated |
|
Arabidopsis thaliana |
| leaf chloroplasts |
contain |
lenticular starch granules |
|
| (BT1, AT5G63160) |
encodes |
ADP-Glc transporter |
Oryza sativa |
| amount of SBEIIa in starch granules |
was correlated significantly and positively with |
branching degree of amylopectin |
|
| primary cause of the mutation, the loss of SBEIIb activity |
is the same |
|
|
| differences between wild-type and GlgC-TM plants in hexose phosphate and ADP-Glc pool sizes in 12:12 conditions |
are attributable to |
different kinetic properties of the native and GlgC-TM enzymes |
Arabidopsis thaliana |
| posttranslational modulation of AGPase activity |
is most likely at the level of |
allosteric regulation |
Arabidopsis thaliana |
| starch content elevation |
occurs in condition that |
activity of starch synthase was kept unchanged |
|
| different mutation patterns in ae − (ae1.1) and ae1.2 |
cause |
different granule-bound protein compositions |
|
| (ATPHO1, PHO1, AT3G23430) |
exhibited |
granule-bound fraction in TRS |
|
| starch synthesis |
was maintained for |
rest of the photoperiod |
Arabidopsis thaliana |
| ADP-Glc pyrophosphorylase |
involved in starch synthesis in |
Arabidopsis thaliana |
Arabidopsis thaliana |
| apyrases |
have been speculated to be involved in |
regulation of starch synthesis |
|
| higher starch levels in cytc mutants |
are mainly the result of |
increased synthesis during the day |
Arabidopsis thaliana |
| starch phosphorylase |
plays important role in |
initiation of starch granules |
Oryza sativa |
| all three small subunit genes |
contribute to |
starch synthesis |
Zea mays |
| GBSSI |
is |
Group 1 gene with up-regulated expression at 9 (DPA, AT5G02470) in superior spikelets |
Oryza sativa |
| SSIIIb |
is |
Group 2 gene with down-regulated expression at 9 (DPA, AT5G02470) in superior spikelets |
Oryza sativa |
| ethephon |
suppressed |
(ATSUS2, SSA, SUS2, AT5G49190) |
Oryza sativa |
| grass endosperm |
contains |
cytosolic form of ADPglucose pyrophosphorylase (AGPase) |
|
| ADPglucose in cyanobacterium |
was committed to |
starch synthesis |
|
| amylose extender (ae) starch |
results from non-functional |
branching enzyme IIb (SBEIIb) |
Zea mays |
| AGPLLZM |
encodes |
large subunit of ADP-glucose pyrophosphorylase (AGPase) |
Zea mays |
| removal of the various AGPase genes known to be expressed in the other tissues along with the removal of Sh2 or (ATBT2, BT2, AT3G48360) function |
does not reduce endosperm starch content to a level appreciably below |
that produced in a single sh2 or (ATBT2, BT2, AT3G48360) mutant |
Zea mays |
| plastidial AGPase |
is encoded by |
agpsemzm and agpllzm loci |
Zea mays |
| starch |
normally accumulates in |
leaves in the light |
|
| regulation of ADP-Glc pyrophosphorylase |
is mediated by |
(NTRC, AT2G41680) |
Arabidopsis thaliana |
| AGPase activity |
was also restored in |
both sorts of lines ( (ADG1, APS1, AT5G48300) and GlgC-TM transgenic lines) |
Arabidopsis thaliana |
| any factor that influences the diel pattern of carbon demand for growth |
may thus indirectly influence |
the rate of starch accumulation during the day |
Arabidopsis thaliana |
| SBE inhibition in TRS hollow starch |
leads to |
ratio of short to long branch chains of amylopectin decreasing from 3.1 to 0.4 |
|
| seven amylopectin biosynthetic enzymes: SSI, SSIIa, SSIIIa, SBEI, SBEIIa, SBEIIb, and (ATPHO1, PHO1, AT3G23430) |
are capable of binding to |
starch granule in the wild type and various mutants |
|
| starch synthase |
shows no significant changes in activity in |
u-ATP9 transgenic lines |
|
| Metabolism in tuber |
is inherently associated with |
starch synthesis |
|
| waxy starch |
results from absence of |
granule-bound starch synthase (GBSS1, AT1G32900) activity |
Zea mays |
| AGPase-independent starch synthesis |
accounts for |
~25% of endosperm starch |
Zea mays |
| genetic differences at loci separate from those encoding AGPase activity |
accounts for |
variation in starch content |
Zea mays |
| double and triple mutants |
lacked |
activity |
Zea mays |
| starch phosphorylase |
can synthesize |
glucan polymers even in elevated phosphate conditions |
Oryza sativa; Escherichia coli |
| another small subunit AGPase gene |
must function in |
embryo |
Zea mays |
| starch |
is synthesized when |
rate of photosynthesis exceeds capacity of leaf to export or store sucrose |
Arabidopsis thaliana |
| induced TPS plants during period from 4 to 6 h after induction |
had ADP-glucose pyrophosphorylase (AGPase) that was, if anything, less reduced (less activated) than |
control plants |
Arabidopsis thaliana |
| abolition of redox sensitivity of ADP-glucose pyrophosphorylase (AGPase) by site-directed mutagenesis of (ADG1, APS1, AT5G48300) |
had surprisingly little effect on |
rate of starch synthesis in plants grown in 12-h photoperiod |
Arabidopsis thaliana |
| Suc-induced changes in redox status of ADP-glucose pyrophosphorylase (AGPase) |
occur via |
mechanism that does not involve trehalose-6-phosphate (Tre6P) |
Arabidopsis thaliana |
| induced and ADP-glucose pyrophosphorylase (AGPase) redox status |
overlapped with at least one, usually more, of the controls at every sampling time |
control plants |
Arabidopsis thaliana |
| induced TPS plants |
accumulated |
a little more starch than noninduced controls by end of day |
Arabidopsis thaliana |
| types of starch synthase (SS) present |
affect |
crystallinity of glucans |
Arabidopsis thaliana |
| AGPase activity in extracts of GlgC-TM plants |
is comparable with |
wild-type plants |
Arabidopsis thaliana |
| redox inactivation of the enzyme |
also acts to prevent |
starch synthesis at night |
Arabidopsis thaliana |
| starch synthesis |
is catalyzed by |
starch synthases (SSs) |
|
| GBSSI amounts and activity |
were not significantly different between |
TQ and TRS |
|
| abundant amylose increase in TRS |
is attributed mainly to |
amylopectin decrease |
|
| novel protein complex formed in the stroma including SBEI, SBEIIa, (ATPHO1, PHO1, AT3G23430) SSI, and SSIIa |
effectively becomes localized to |
starch granules in ae − |
|
| amount of SBEI, SBEIIa, and SBEIIb decrease from polygonal to hollow granules |
is in agreement with |
branching degree of amylopectin decreasing and branch-chain length increasing gradually |
|
| production of ADP-glucose (ADPG) |
increases |
synthesis of starch |
higher plants |
| basal levels of trehalose-6-phosphate (Tre6P) in plants |
may be |
already saturating the putative mechanism by which Tre6P affects redox status of ADP-glucose pyrophosphorylase (AGPase) |
Arabidopsis thaliana |
| induced TPS plants |
showed no consistent evidence of increase in |
ADP-glucose pyrophosphorylase (AGPase) activation |
Arabidopsis thaliana |
| trehalose-6-phosphate (Tre6P) |
cannot be ruled out to act to promote |
activation of ADP-glucose pyrophosphorylase (AGPase) |
Arabidopsis thaliana |
| (ATPGMP, PGM, PGM1, STF1, AT5G51820) mutant |
had ADP-glucose pyrophosphorylase (AGPase) that was considerably more reduced than |
wild-type plants |
Arabidopsis thaliana |
| ADP-glucose pyrophosphorylase (AGPase) |
catalyzes |
conversion of glucose 1-phosphate to ADP-glucose |
|
| low AGPase activity restriction |
is at least partially relieved in |
field-grown plants |
|
| expansion of starch synthase genes |
probably elevated |
starch levels within the rhizome |
Nelumbo nucifera |
| Sacred lotus (Nelumbo nucifera) rhizome |
contains elevated |
starch |
Nelumbo nucifera |
| potato genome |
lacks significant gene expansions within |
starch-synthesis pathway |
Solanum tuberosum |
| leaves lacking the large subunit or small subunit previously known to be expressed in the leaf |
exhibit |
drastic loss in leaf starch |
Zea mays |
| transcripts for other AGPase subunits |
are present in leaves but have |
little or no physiological significance |
Zea mays |
| residual starch in endosperm |
may arise from |
ADP-glucose synthesized via sucrose synthase |
Zea mays |
| all three small subunit genes |
contribute to |
embryo AGPase activity |
Zea mays |
| Arabidopsis thaliana lacking (GPT2, AT3G53320) |
accumulates less |
starch in high light than wild-type |
Arabidopsis thaliana |
| adenosine diphosphate glucose pyrophosphorylase |
is essential for |
maize viability |
Zea mays |
| granule-bound starch synthase (GBSS) |
affects amylose synthesis by catalyzing |
glucosyl transfer from ADP-glucose to growing α-1,4-D-glucan chain |
|
| significant expansion of starch-related genes (GBSS genes) in sacred lotus |
may explain |
starch enrichment within rhizome tissue |
|
| genetic expansion of starch-related genes (GBSS genes) in sacred lotus |
may have resulted in |
crisp taste associated with sacred lotus rhizome |
|
| starch metabolism |
is controlled by |
adenosine diphosphate glucose pyrophosphorylase |
Zea mays |
| multi-level transcriptional regulation atlas |
involves |
miRNA |
Zea mays |
| starch content and AGPase activity in heterozygous relative to control grains |
were also reduced but reductions were less severe than for grains from |
homozygous plants |
Triticum aestivum |
| starch synthase isoforms |
share |
common catalytic domain |
|
| enhanced ADP-Glc synthesis and import into amyloplasts |
results in only limited |
carbon flow into starch |
|
| three SBEs reduction by more than 70% in barley |
results in |
pure amylose derived |
Hordeum vulgare |
| SBE inhibition in TRS hollow starch |
leads to |
amylopectin content decreasing from 76.3% to 22.6% |
|
| amount of SBEIIa in starch granules |
was correlated negatively with |
amylopectin branch chain length |
|
| transgenic plants expressing deregulated GlgC-TM |
had maximum catalytic activities of AGPase higher than |
wild-type plants |
Arabidopsis thaliana |
| N-terminal extensions of (SS3, AT1G74000) |
confer higher affinity for |
glucan substrates |
|
| SBE values less than transition points |
causes |
amylopectin content and molecular structure to change substantially |
|
| SSs and SBEs |
purportedly attach to the surface of |
starch granule |
|
| ADP-glucose pyrophosporylase (AGPase) |
is not found in |
diatom plastids |
|
| abolition of starch production |
reduces sensitivity of |
seedling growth to trehalose |
Arabidopsis thaliana |
| BGC1 gene |
is orthologous to |
FLOURY ENDOSPERM6 (FLO6) |
Oryza sativa; Hordeum vulgare |
| plants expressing deregulated bacterial adenosine 5′-diphosphate-glucose pyrophosphorylase (AGPase) |
show compromised |
adjustment of starch synthesis to daylength |
Arabidopsis thaliana |
| ADP-Glc levels in wild-type plants |
were high during the day (at ZT3) and essentially undetectable at night |
in 12:12 and 6:18 conditions |
Arabidopsis thaliana |
| granule-associated proteins of four heterogeneous starch granules |
indicate |
gradually decreasing dosages of SBEI, SBEIIa, and SBEIIb |
Oryza sativa |
| allelic variant of the sugray-2 (su2) mutation expressing a catalytically inactive form of SSIIa in maize |
enables |
trimeric assembly of SSI, SSIIa, and SBEIIb in the stroma of amyloplasts |
|
| no difference in SSIIa |
was detected |
|
|
| increased levels of ADP-glucose via AGPase |
inhibits |
phosphorylase function during later stages of seed development |
Oryza sativa |
| deregulated GlgC-TM enzyme from E. coli |
was used to replace |
native AGPase |
Arabidopsis thaliana |
| (SS3, AT1G74000) |
is important for |
establishing proper amylopectin structure |
|
| TRS (transgenic resistant starch rice line) |
has |
amylose content of approximately 60% |
Oryza sativa |
| fall in concentration of triose phosphate in the chloroplast |
causes |
rate of starch synthesis decreases |
|
| end-of-day starch contents in several GlgC-TM lines |
were 60% to 100% of |
wild-type values |
Arabidopsis thaliana |
| transformation with the empty vector |
did not restore |
starch synthesis |
Arabidopsis thaliana |
| branching enzyme |
transfers |
part of linear glucan chain |
|
| lack of sucrose accumulation |
prevents |
up-regulation of starch synthesis |
Arabidopsis thaliana |
| starch synthesis |
is catalyzed by |
starch branching enzymes |
|
| gradually decreasing dosages of SBEI, SBEIIa, and SBEIIb |
are responsible for |
differences in molecular structure of granules |
Oryza sativa |
| SSI |
plays a critical role in |
generating chains of DP 8-12 from chains of DP 6-7 that emerge from the branch points of amylopectin |
|
| mutations preventing starch synthesis |
prevent |
chlorosis of (MEX1, RCP1, AT5G17520) |
Arabidopsis thaliana |
| (AtSS2, SS2, AT3G01180) mutant plants |
contained |
small amounts of phytoglycogen in addition to aberrant starch |
Arabidopsis thaliana |
| ss2ss3 plants |
retained |
other starch synthase isoforms |
Arabidopsis thaliana |
| plants expressing deregulated AGPase in short days |
had lower allocation of carbon into starch and greater reductions in growth rate than |
wild-type plants |
Arabidopsis thaliana |
| amount of SBEIIa in starch granules |
was correlated significantly and positively with |
short branch chain of amylopectin |
|
| plant SS isoforms |
have |
N-terminal extensions of different lengths |
|
| starch |
is synthesized as |
discrete semicrystalline granules having a layered organization with alternating semicrystalline and amorphous growth rings |
|
| gradual reduction of SBEs from TRS polygonal to hollow granules |
led to |
greater accumulation of amylose in lamellae |
|
| SSIIa |
preferentially elongates |
DP 6-11 to DP 13-28 |
|
| SSIIa trafficking other enzymes into the granule |
leads to |
SSIIa being unbiasedly bound to the granule |
|
| (ATPHO1, PHO1, AT3G23430) |
is not responsible for |
different amylose accumulation in four TRS heterogeneous starch granules |
|
| ss2ss3isa triple mutants |
restored |
glucan production |
Arabidopsis thaliana |
| plants containing mutant forms of endogenous adenosine 5′-diphosphate-glucose pyrophosphorylase (AGPase) with altered allosteric regulatory properties |
show compromised |
adjustment of starch synthesis to daylength |
Arabidopsis thaliana |
| (ADG2, APL1, AT5G19220) mutant |
lacks |
(ADG2, APL1, AT5G19220) subunit of AGPase |
Arabidopsis thaliana |
| changes in starch content of biomass during culture |
are shown in |
Figure 3B |
Chlamydomonas reinhardtii |
| differences in rates of sucrose synthesis at the start of the day |
are expected to influence |
rate of starch synthesis |
|
| N-terminal region of STARCH SYNTHASE 4 (ATSS4, SS4, SSIV, AT4G18240) |
is unique among |
starch synthase isoforms |
Arabidopsis thaliana |
| FLO4 |
encodes |
pyruvate orthophosphate dikinase |
Oryza sativa |
| amount of SBEIIa in starch granules |
was correlated significantly and positively with |
extra long chain of amylopectin |
|
| decreased photosynthetic capacity |
results in a decrease of |
carbon available for starch synthesis |
Arabidopsis thaliana |
| ADP-Glc level in GlgC-TM line C4 |
was 50% lower than |
wild-type plants |
Arabidopsis thaliana |
| low growth rate of the transgenic plants |
is at least in part a direct consequence of |
low starch levels and hence reduced carbon availability at night |
Arabidopsis thaliana |
| maize (Zea mays) amylose extender (ae) mutant |
has |
amylose content increased to approximately 60% from 30% |
Zea mays |
| BRITTLE1 (BT1, AT5G63160) gene overexpression in up-regulated AGPase background |
generates rice line with |
enhanced ADP-Glc synthesis and import into amyloplasts |
|
| Arabidopsis thaliana leaves |
synthesize starch slower in |
long days |
Arabidopsis thaliana |
| adg1-1 mutant |
has |
no accumulation of AGPase large subunits |
Arabidopsis thaliana |
| redox and allosteric regulatory properties of AGPase |
effectively prevent |
synthesis of ADP-Glc at night |
Arabidopsis thaliana |
| (SS3, AT1G74000) |
is proposed to synthesize |
long, cluster-spanning amylopectin chains |
|
| amylose content of starch |
potentially correlates with |
limited physical space available within the matrix of amylopectin |
|
| amount of SBEIIb in starch granules |
was correlated significantly and positively with |
extra long chain of amylopectin |
|
| different amounts of SBEI, SBEIIa, and SBEIIb distributed in the four heterogeneous starch granules |
caused |
different binding amounts of SSI |
|
| stromules |
do not appear to be |
general site of starch synthesis in other tissues |
|
| modification of allosteric properties |
can reduce |
the capacity for adjustment of starch synthesis in response to daylength |
Arabidopsis thaliana |
| amount of SBEIIb in starch granules |
was correlated negatively with |
amylopectin branch chain length |
|
| isa mutant |
accumulates primarily |
phytoglycogen in leaf mesophyll cells |
Arabidopsis thaliana |
| retention of larger proportion of triose phosphate in the chloroplast |
leads to |
use of triose phosphate for starch synthesis |
|
| adenosine 5′-diphosphate-glucose pyrophosphorylase (AGPase) |
is |
starch synthesis enzyme |
Arabidopsis thaliana |
| regulatory properties of adenosine 5′-diphosphate-glucose pyrophosphorylase (AGPase) |
are required for |
adjustment of leaf starch synthesis in different photoperiods |
Arabidopsis thaliana |
| mutations blocking starch synthesis |
reduce |
plant growth |
Arabidopsis thaliana |
| growth rate at end of preceding night |
determines in part |
rate of starch synthesis |
Arabidopsis thaliana |
| ADP-Glc levels in GlgC-TM plants |
tended to be lower during the day (at ZT3) than |
those in wild-type plants |
Arabidopsis thaliana |
| residual AGPase activity in (ADG2, APL1, AT5G19220) mutant |
is due to |
either the formation of active (ADG1, APS1, AT5G48300) homotetramers or the association of with one of the other three minor large subunits ( (APL2, AT1G27680) (APL3, AT4G39210) and (APL4, AT2G21590) ) |
Arabidopsis thaliana |
| GBSS (granule-bound starch synthase) |
synthesizes |
amylose |
|
| ratio of amylose and amylopectin content in TRS |
undergoes great changes |
|
|
| starch synthase (SS) mutants lacking (ATSS1, SS1, AT5G24300) (AtSS2, SS2, AT3G01180) and (SS3, AT1G74000) |
vary |
chain length distribution |
Arabidopsis thaliana |
| (ATSS1, SS1, AT5G24300) |
is important for |
establishing proper amylopectin structure |
|
| adjustment of starch synthesis to daylength |
is dependent upon |
posttranslational modulation of AGPase activity |
Arabidopsis thaliana |
| ADP-Glc synthesis at night in transgenic plants with unregulated AGPase |
may adversely affect |
growth rates of plants expressing unregulated AGPase |
Arabidopsis thaliana |
| Arabidopsis thaliana (ATSS4, SS4, SSIV, AT4G18240) mutant |
accumulates very high levels of |
ADP-Glc |
Arabidopsis thaliana |
| gradually increasing amylose content in four TRS heterogeneous granules |
is due to |
decreased amylopectin synthesis and enhanced amylose synthesis |
Oryza sativa |
| four TRS heterogeneous granules |
have |
GBSSI amounts not significantly different from control |
Oryza sativa |
| NADP-thioredoxin reductase C |
is known to activate |
ADP (GLC, AT1G65450) pyrophosphorylase |
|
| GBSS-I |
is regulated |
post-transcriptionally |
Triticum aestivum |
| single AGPase mutants |
did not reduce |
ovary starch content |
Zea mays |
| heat labile activity |
is preferentially reduced in |
sh2 and (ATBT2, BT2, AT3G48360) mutants |
Zea mays |
| SSIIa mutants |
exhibit a characteristic increase in |
shorter glucan chains (DP 7–10) |
Triticum aestivum; Hordeum vulgare; Oryza sativa; Zea mays |
| GRANULE BOUND STARCH SYNTHASE (GBSS) |
synthesizes |
amylose |
|
| ZmMYB138 |
affects transcriptional activities of |
Du1 / Wx genes |
Zea mays |
| gradually decreasing SBEI, SBEIIa, and SBEIIb |
might release more space for |
amylose synthesis |
|
| fourth gene |
encodes |
large subunit of ADP-glucose pyrophosphorylase (AGPase) |
Zea mays |
| loss of the small subunit known to be expressed in the embryo (agpsemzm) |
abolishes |
heat-stable activity |
Zea mays |
| changes in level of trehalose-6-phosphate (Tre6P) on their own |
do not play dominant role in regulating |
redox status of ADP-glucose pyrophosphorylase (AGPase) in leaves |
Arabidopsis thaliana |
| ADP-glucose |
is transported into |
plastid |
Zea mays |
| (ATBT2, BT2, AT3G48360) protein |
functions in |
amyloplast |
Zea mays |
| (AKIN10, KIN10, SnRK1, SnRK1α1, SNRK1.1, AT3G01090) (sucrose non-fermenting related kinase 1) |
is involved in control of |
starch synthesis |
|
| (GWD, GWD1, SEX1, SOP, SOP1, AT1G10760) mediated glucan phosphorylation |
is involved in |
starch synthesis |
Arabidopsis thaliana |
| (ELF3, PYK20, AT2G25930) mutants |
showed higher rate of starch accumulation in short photoperiods compared with |
long photoperiods |
Arabidopsis thaliana |
| SSIIIa mutation in the japonica background (ssIIIa / Waxy b) |
inhibits |
amylopectin synthesis |
|
| more than one ADP-glucose pyrophosphorylase (AGPase) isoform |
functions in |
embryo and ovary |
Zea mays |
| Shrunken-2 (Sh2) |
is expressed in |
endosperm |
Zea mays |
| (ATBT2, BT2, AT3G48360) ;agpsemzm double mutant |
has embryo AGPase activity reduced by 50% via |
loss of agpsemzm function |
Zea mays |
| ovaries lacking the function of the small AGPase subunits known to be expressed in the leaf and embryo |
reduced |
total ovary starch content by approximately 70% |
Zea mays |
| endosperm starch |
cannot be reduced below 25% wild-type levels in double and triple mutant combinations when AGPase activity is approximately 1% of wild-type |
AGPase-independent starch synthesis |
Zea mays |
| residual activity |
was found in |
all combinations assayed |
Zea mays |
| trehalose-6-phosphate (Tre6P) |
mediates |
sugar-induced increases in rate of starch synthesis via redox activation of ADP-glucose pyrophosphorylase (AGPase) |
Arabidopsis thaliana |
| increase in ratio of 3-phosphoglycerate (3PGA) to orthophosphate (Pi) in chloroplast stroma |
stimulates |
starch synthesis via allosteric activation of ADP-glucose pyrophosphorylase (AGPase) |
Arabidopsis thaliana |
| AGPase |
transcript levels are higher in |
embryos of WH5557 |
Brassica napus |
| sucrose synthases of maize seed |
prefer UDP as substrate but can also use ADP as substrate and synthesize |
ADP-glucose |
Zea mays |
| loss of agpsemzm function |
leads to 50% reduction in |
AGPase activity |
Zea mays |
| agpslzm (LeafS) |
is expressed in |
leaf |
Zea mays |
| AGPase isoforms |
function in |
various tissues of maize |
Zea mays |
| other enzymes in endosperm |
can synthesize |
ADP-glucose |
Zea mays |
| (ATBT2, BT2, AT3G48360) protein |
functions in more than just |
endosperm |
Zea mays |
| loss of agpsemzm function |
does not totally abolish |
embryo AGPase activity |
Zea mays |
| small and large subunits of ADP-glucose pyrophosphorylase (AGPase) |
are expressed at abundant levels in |
maize leaf |
Zea mays |