| Ttparc6 BC aabb double mutant |
has significant increase in mean diameter of B-type granules |
B-type granule diameter |
Triticum turgidum ssp. durum |
| Ttparc6 mutants |
produce grains with |
altered starch granule size distribution |
Triticum turgidum ssp. durum |
| Agpsemzm |
encodes |
AGPase small subunit (SS) |
Zea mays |
| agpsemzm-Mu1 homozygous mutant embryo |
contains approximately 46% of |
starch content |
Zea mays |
| GS or C-terminal region of (ATSS4, SS4, SSIV, AT4G18240) alone |
partially complement |
(ATSS4, SS4, SSIV, AT4G18240) mutant phenotype |
Arabidopsis thaliana |
| SBEIIb dosage |
is negatively related to |
amylopectin long chains |
|
| dynamic deposition mode of starch during development in control |
includes relatively low accumulation until maturity along with dehydration process |
starch accumulation period |
|
| distribution of SSI in four types of granules |
shows similar tendency to |
distribution of three SBE isoforms |
|
| (ARC6H, ATCDP1, CDP1, PARC6, AT3G19180) mutant |
does not affect |
starch content |
Triticum turgidum |
| Ttparc6-1 aabb and Ttparc6 BC aabb double mutants |
have large significant increase in |
B-type granule content |
Triticum turgidum ssp. durum |
| Ttparc6 double mutants |
have amylose content indistinguishable from |
wild-type and wild-type segregants |
Triticum turgidum ssp. durum |
| wild-type, AlcR, and TPS control plants sprayed with water |
accumulated |
62 to 79 µmol [Glc] g−1 fresh weight of starch by the ED |
Arabidopsis thaliana |
| (AtSS2, SS2, AT3G01180) and ss2ss3 mutants |
had |
contrasting starch- and phytoglycogen-accumulating phenotypes overall |
Arabidopsis thaliana |
| increase in ADP-Glc in ss2ss3 |
is small compared to |
other mutants such as (ATSS4, SS4, SSIV, AT4G18240) and ss3ss4 |
Arabidopsis thaliana |
| agpllzm mutant |
causes reduced |
AGPase activity in specific tissues |
Zea mays |
| AGPase distribution in wheat endosperm at later developmental stages |
is |
40% plastidial |
Triticum aestivum |
| Nonmutant tissue |
relies on contributions from |
both plastidial and cytosolic AGPase |
Zea mays |
| malate |
plays important role in |
starch accumulation |
Solanum lycopersicum; Prunus persica; Capsicum annuum; Fragaria x ananassa |
| reduction of SBE isoforms |
may be expected to reduce |
overall rate of amylopectin synthesis |
|
| (ATPHO1, PHO1, AT3G23430) |
displays accumulating trend in |
TRs |
|
| increased trehalose-6-phosphate (Tre6P) levels during the day |
is not linked to reductive activation of |
ADP-glucose pyrophosphorylase (AGPase) |
Arabidopsis thaliana |
| Ttparc6 single homeolog mutants |
have intermediate change in |
granule size distribution |
Triticum turgidum ssp. durum |
| Ttparc6-2 aabb mutant amyloplasts |
have increased size compared with |
wild-type amyloplasts |
Triticum turgidum ssp. durum |
| very large A-type granules in Ttparc6 double mutants |
have disrupted |
Maltese cross |
Triticum turgidum ssp. durum |
| plants harvested in the light |
contained |
0.5 to 4.0 nmol g−1 fresh weight ADPG |
Arabidopsis thaliana |
| AGPase α2β2 heterotetramer |
comprises |
large subunit (LS) and small subunit (SS) |
|
| Three additional genes |
encode |
plastidial AGPase large subunit (LS) |
Oryza sativa; Zea mays |
| Agpslzm gene |
encodes |
AGPase small subunit |
Zea mays |
| YFP-GS_1 and YFP-GS_2 |
showed complementation in terms of |
starch accumulation |
Arabidopsis thaliana |
| N-terminal region of (ATSS4, SS4, SSIV, AT4G18240) by itself |
appears to have no effect on |
starch biosynthesis |
Arabidopsis thaliana |
| Ttparc6-2 + cTPmCherry aabb double mutant |
has amyloplast size drastically increased in |
endosperm sections at 16 DAF |
Triticum turgidum ssp. durum |
| adenosine 5-diphosphate-glucose pyrophosphorylase (AGPase) |
is composed of |
large and small subunits |
|
| Three transcripts encoding AGPase small subunit (SS) in maize endosperm |
could specify |
plastidial AGPase small subunit (SS) proteins |
Zea mays |
| YFP-SS4C construct |
contains |
GT1 domain |
Arabidopsis thaliana |
| SBEs in TRS |
are inhibited gradually along with |
endosperm development in TRS |
|
| sbe2b single mutant |
shows |
reduction in SSI activity |
|
| weak and diffuse signals of SBEs in other three regions of TRS |
are barely detected in |
regions II, III, and IV of TRS endosperm |
|
| mutation of the gene encoding chloroplastic α-AMYLASE3 (AMY3, ATAMY3, AT1G69830) |
restored |
starch granule biosynthesis in all cell types examined |
Arabidopsis thaliana |
| starch synthase (SS) activity biased toward longer external chains |
promotes |
starch granule biosynthesis |
|
| glucans from ss1isa mutant |
have fewer short chains (DP 7–10) but more longer chains (DP 13–21) compared to |
isa mutant glucans |
Arabidopsis thaliana |
| differences in chain length |
were consistent in |
all backgrounds analyzed |
Arabidopsis thaliana |
| adenosine 5-diphosphate-glucose pyrophosphorylase (AGPase) |
catalyzes |
synthesis of ADP-glucose (ADP- (GLC, AT1G65450) ) and pyrophosphate from ATP and glucose 1-phosphate ( 1-P) |
|
| agpllzm gene |
is necessary for |
accumulation of normal starch levels in leaf |
Zea mays |
| AGPSLZM mRNA |
is detected in |
mature leaf tissue |
Zea mays |
| supernumerary granules |
were |
tiny and of irregular shape |
Arabidopsis thaliana |
| soluble enzyme proteins |
have dynamic deposition or maintain sustainable level in |
granules throughout starch development |
|
| loss of debranching enzyme (DBE) of the (ARA1, ATISA1, ISA1, AT4G16130) class |
results in production of |
soluble glucan (phytoglycogen) in place of starch |
Chlamydomonas reinhardtii; Arabidopsis thaliana; Zea mays; Oryza sativa; Hordeum vulgare |
| ss2ss3 mutant |
has total levels strongly |
reduced |
Arabidopsis thaliana |
| AGPase activity alteration by transgenic means |
resulted in |
elevated yields |
Oryza sativa; Zea mays; Triticum aestivum; Solanum tuberosum |
| AGPLLZM, AGPSLZM, AGPLEMZM, AGPSEMZM, and BT2b |
are predicted to localize to |
plastids |
Zea mays |
| agpsemzm-Mu1 homozygous embryo |
shows reduced total |
AGPase activity |
Zea mays |
| fate of imported Glc-1-P and Glc-6-P |
must be split between |
starch sink and nonstarch metabolism |
Zea mays |
| GS and SS4C |
contain |
glycosyltransferase 5 (GT5) domain |
|
| GBSSI amount and activity |
is almost the same as |
GBSSI amount and activity in TQ |
|
| two genes |
encode |
AGPase small subunit (SS) proteins |
Hordeum vulgare; Oryza sativa; Triticum aestivum |
| Type 1 gene (ATBT2, BT2, AT3G48360) |
generates |
BT2a and BT2b transcripts |
Zea mays |
| Different gene agpslzm |
generates |
type 1b transcript |
Zea mays |
| agpsemzm-Mu1 homozygous endosperm from kernels harvested 20 DAP |
contains approximately 7% less |
starch |
Zea mays |
| chimeric construct |
contains |
full-length Agrobacterium tumefaciens GS |
Arabidopsis thaliana |
| starch granules |
contains |
amylose |
|
| Arabidopsis isa1isa2isa3lda quadruple mutant |
makes no |
starch granules |
Arabidopsis thaliana |
| (ATBT2, BT2, AT3G48360) mRNA |
is highly abundant in |
endosperm |
Zea mays |
| reduced starch synthesis and degradation rates in (ATSS4, SS4, SSIV, AT4G18240) mutants |
may be consequence of |
deposition as fewer, enlarged, and rounded granules |
Arabidopsis thaliana |
| (PTST2, AT1G27070) |
provides |
long malto-oligosaccharide substrates |
|
| SBEs |
are prone to forming |
multisubunit complexes with SSs, DBEs, and (ATPHO1, PHO1, AT3G23430) in soluble fraction of amyloplasts |
|
| three SBEs in TQ |
show relatively low rate until maturity |
granule-bound SBE accumulation |
|
| ISOAMYLASE1-ISOAMYLASE2 (ISA1-ISA2) |
instead of trimming it to |
amylopectin |
Arabidopsis thaliana |
| balance of specific starch synthase (SS) activities |
is important for |
starch granule formation |
|
| (ARA1, ATISA1, ISA1, AT4G16130) homomultimeric complex |
is found only in |
cereal endosperms |
|
| Agpsemzm gene |
is necessary for |
accumulation of normal starch levels in embryo |
Zea mays |
| agpllzm mutant |
decreases endosperm starch by approximately |
endosperm starch levels |
Zea mays |
| Type 1b transcript |
is predicted to encode |
plastidial AGPase small subunit (SS) |
Hordeum vulgare; Oryza sativa; Triticum aestivum |
| genetic analyses |
showed that |
multiple genes encode AGPase subunits in leaf and embryo |
Zea mays |
| agpllzm-Ds1 homozygous leaf |
shows strongly reduced total |
AGPase activity |
Zea mays |
| cytosolic AGPase |
could be adapted for maximal |
ADPGlc formation |
Zea mays |
| dark bands |
were visible in |
all lines |
Arabidopsis thaliana |
| (ATSS4, SS4, SSIV, AT4G18240) C-terminal region |
appears to confer capacity to interact with |
(PTST2, AT1G27070) |
|
| dynamic deposition mode of starch during development in control |
includes fast seed-filling stage from 4 to 10 DAF |
starch accumulation period |
|
| three SBEs bound with granule in TQ |
behave consistently with |
dynamic deposition mode of starch in TQ |
|
| tobacco and Arabidopsis mutants defective in plastidial isozyme of phosphoglucomutase (ATPGMP, PGM, PGM1, STF1, AT5G51820) |
exhibit |
starch-free phenotype |
Nicotiana tabacum; Arabidopsis thaliana |
| Arabidopsis thaliana |
contains |
four starch synthase (SS) isoforms (SS1–SS4) |
Arabidopsis thaliana |
| loss of starch synthase (SS) in a debranching enzyme (DBE) mutant background |
altered |
ratio of starch to phytoglycogen |
Zea mays |
| ss1isa mutant |
shows frequent occurrence of starch granules in |
mesophyll cell chloroplasts |
Arabidopsis thaliana |
| debranching in (AtSS2, SS2, AT3G01180) mutant |
may happen to an extent but still allows |
crystallization (or partial crystallization) of most glucans made by other SS s |
Arabidopsis thaliana |
| cereal endosperm AGPase |
is located in |
amyloplasts |
|
| Agpsemzm |
null mutations in |
agpsemzm mutant |
Zea mays |
| AGPLLZM |
encodes |
AGPase large subunit (LS) |
Zea mays |
| AGPL3 |
encodes |
AGPase large subunit (LS) |
Zea mays |
| zymograms |
are not able to detect |
all glucosyl transferase activities |
Arabidopsis thaliana |
| SS4N-GS-YFP/YFP-SS4N-GS plants |
had iodine-staining patterns |
very similar to wild type and SS4-YFP control plants |
Arabidopsis thaliana |
| (ATSS4, SS4, SSIV, AT4G18240) in vivo substrate affinity |
may be modulated by |
(PTST2, AT1G27070) |
|
| heterogeneous starches in TRS endosperm |
show gradually decreasing |
amylopectin content |
Oryza sativa |
| ss2ss3isa mutant |
has no starch granules despite |
(AtSS2, SS2, AT3G01180) and ss2ss3 phenotypes being quite distinct |
Arabidopsis thaliana |
| Three transcripts encoding AGPase small subunit (SS) |
have been detected in |
maize endosperm |
Zea mays |
| SH2 and AGPSEMZM |
coexpression yields |
dark iodine stain indicating functional AGPase |
Zea mays; Escherichia coli |
| agpllzm-Ds1 mutants |
show remnant |
AGPase activity |
Zea mays |
| GS and SS4C |
contain |
glycosyltransferase 1 (GT1) domain |
|
| expression of either GS or SS4C in (ATSS4, SS4, SSIV, AT4G18240) background |
facilitated formation of |
more starch granules |
Arabidopsis thaliana |
| supernumerary granules |
often had |
smooth surfaces |
Arabidopsis thaliana |
| three SS4-YFP lines |
also produced |
multiple wild-type-like granules per chloroplast section |
Arabidopsis thaliana |
| high-amylose rice line (TRS) |
contains |
elongated starch |
Oryza sativa |
| SBE dosage |
relates to |
morphological architecture of heterogeneous starches |
Oryza sativa |
| length of external chains |
is key factor in |
production of crystallization-competent glucan |
Arabidopsis thaliana |
| suppression of (SS3, AT1G74000) expression |
results in granules with |
fractured appearance |
Solanum tuberosum |
| (ARA1, ATISA1, ISA1, AT4G16130) homomultimeric complex |
is form of |
isoamylase activity |
Zea mays |
| β-limit CLD s in ss2ss3 double mutant |
is enriched in |
short chains |
Arabidopsis thaliana |
| findings from this study |
provide important step forward toward understanding how |
plants produce semicrystalline starch granules |
|
| Third gene encoding AGPase small subunit (SS) |
is present in maize owing to |
duplication of ancestral type 1 gene |
Zea mays |
| BT2a |
functions only with |
Sh2 |
Zea mays; Escherichia coli |
| AGPSEMZM absence |
causes decrease of approximately 50% in |
starch content |
Zea mays |
| chimeric construct |
contains |
N-terminal region of (ATSS4, SS4, SSIV, AT4G18240) |
Arabidopsis thaliana |
| multiple ellipsoid starch granules |
consistent with |
flattened, lenticular shape of granules |
Arabidopsis thaliana |
| expression of SS4N-GS fusion constructs |
almost fully complemented |
(ATSS4, SS4, SSIV, AT4G18240) mutant phenotype |
Arabidopsis thaliana |
| (PTST2, AT1G27070) and/or (PTST3, AT5G03420) proteins |
deliver |
substrates to SS4C |
|
| granule-bound starch synthase I (GBSSI) |
amounts in four heterogeneous starches are not significantly different from each other |
four heterogeneous starches |
Oryza sativa |
| SBEs in TQ |
shows increased protein level until 10 DAF but then decreasing trend |
endosperm development in TQ |
|
| type 1 genes |
generate |
type 1a and type 1b transcripts |
Hordeum vulgare; Oryza sativa; Triticum aestivum |
| Complementary DNA (cDNA) from type 2 gene |
was cloned from |
embryo tissue |
Zea mays |
| agpllzm-Ds1 homozygous mutant leaf |
does not stain appreciably with |
iodine |
Zea mays |
| AGPLLZM |
is capable of generating enzymatic activity when combined with |
at least one partner subunit |
Zea mays |
| GS |
is homologous to |
C-terminal half of (ATSS4, SS4, SSIV, AT4G18240) |
|
| supernumerary granules |
were not observed in |
SS4-YFP lines |
Arabidopsis thaliana |
| heterogeneous starches in TRS endosperm |
show gradually increasing |
ratio of amylopectin long chain |
Oryza sativa |
| amylopectin deposition modes |
are significantly different between |
TRS and TQ |
|
| amylopectin increase in TQ |
is mild |
following stage after 10 DAF |
|
| SBE isoforms |
are responsible for |
branching of amylopectin |
|
| (ATPHO1, PHO1, AT3G23430) in three outer granules |
reveals thick band |
(ATPHO1, PHO1, AT3G23430) abundance |
|
| addition of TREHALOSE-6-PHOSPHATE (T6P) to isolated pea leaf chloroplasts |
causes |
activation of ADP-glucose pyrophosphorylase (AGPase) |
Pisum sativum |
| loss of (SS3, AT1G74000) |
is more apparent when |
other SS s are also missing |
Arabidopsis thaliana |
| SH2 mRNA |
is detected in |
developing embryo |
Zea mays |
| concentrations of the AGPase products and substrates in barley amyloplasts at mid development |
are such that the plastidial enzyme operates near |
equilibrium |
Hordeum vulgare |
| SS4N-YFP plants |
phenocopied |
(ATSS4, SS4, SSIV, AT4G18240) mutant |
Arabidopsis thaliana |
| interference with endogenous enzyme activities |
restricts |
rate of starch biosynthesis |
|
| Association of GS with starch granule surface |
might crowd out |
chain elongating, branching and debranching enzymes |
|
| three SBEs in TQ |
show fast rate from 4 to 10 DAF |
granule-bound SBE accumulation |
|
| ss2ss3 mutant |
has almost one-half of measured glucans as |
soluble glucans |
Arabidopsis thaliana |
| Agpslzm |
is capable of generating enzymatic activity when combined with |
at least one partner subunit |
Zea mays |
| Agpsemzm |
is capable of generating enzymatic activity when combined with |
at least one partner subunit |
Zea mays |
| differences in granule morphology |
were accompanied by |
changes in amylopectin structure |
Arabidopsis thaliana |
| (ATSS4, SS4, SSIV, AT4G18240) C-terminal domains |
confer |
catalytic activity for glucan chain elongation |
|
| SSIIa |
maintains almost the same level in |
four heterogeneous granules |
|
| some cell types in isa1-deficient mutants |
still produce |
some starch |
Arabidopsis thaliana |
| (AtSS2, SS2, AT3G01180) mutant mesophyll |
contains mixture of |
unusually big starch granules, intermediate particles, and phytoglycogen |
Arabidopsis thaliana |
| elevated levels of water-soluble polysaccharides in ss2ss3 mutant |
were noted in earlier report under |
some growth conditions |
Arabidopsis thaliana |
| low levels in ss2ss3 |
are not due to |
incapacity for synthesis per se |
Arabidopsis thaliana |
| data from this study |
reinforce |
importance of normal glucan metabolism on plant growth and vitality |
Arabidopsis thaliana |
| cereal endosperm AGPase |
is located in |
cytosol |
|
| five candidate genes |
could encode |
subunits of plastidial AGPase |
Zea mays |
| Total endosperm AGPase activity in cytosolic AGPase null mutants |
is reduced, with approximately 12% to 25% remaining |
residual AGPase activity |
Zea mays; Hordeum vulgare; Oryza sativa |
| Type 1b mRNA |
is |
highly abundant |
Hordeum vulgare |
| AGPLLZM and BT2b |
coexpression fails to yield |
functional AGPase activity |
Zea mays; Escherichia coli |
| dynamic deposition mode of starch during development in control |
includes moderate accumulation from 10 to 15 DAF |
starch accumulation period |
|
| other factors |
can affect |
partitioning between phytoglycogen and starch |
|
| loss of (AtSS2, SS2, AT3G01180) in ss1ss2isa background |
appears to dominate over |
effect of losing (ATSS1, SS1, AT5G24300) |
Arabidopsis thaliana |
| depletion of longer external chains |
is likely major factor in |
increased solubility of glucans in (AtSS2, SS2, AT3G01180) and ss2ss3 |
Arabidopsis thaliana |
| branching pattern |
determines whether |
crystallization-competent glucan is produced |
Arabidopsis thaliana |
| agpllzm-Ds1 (Dissociation1) allele |
is isolated from |
sequence-indexed Ds transposon population |
Zea mays |
| YFP-GS lines |
varied considerably in |
starch content at end of day |
Arabidopsis thaliana |
| loss of all debranching activity in Arabidopsis |
still allows |
starch granule formation under some circumstances |
Arabidopsis thaliana |
| glucans in ss2ss3 mutant |
appeared more similar to |
those in isa mutants |
Arabidopsis thaliana |
| Type 1a transcript from (ATBT2, BT2, AT3G48360) gene |
operates in |
endosperm |
Zea mays |
| glucans from ss2isa mutant |
show more short chains (DP 5–9) and fewer longer chains (DP 11–18) compared to |
isa mutant glucans |
Arabidopsis thaliana |
| Agpslzm |
encodes |
AGPase small subunit (SS) |
Zea mays |
| reduction in starch when plastidial AGPase was affected by agpsemzm-mutation |
is consistent with |
AGPase function in starch synthesis |
Zea mays |
| extra amylose coupled with altered amylopectin structure |
possibly leads to |
morphological changes in heterogeneous granules |
Oryza sativa |
| isoamylase activity |
is important for |
semicrystalline starch granule formation |
|
| brittle2 |
encodes |
cytosolic AGPase |
Zea mays |
| Null mutations in genes encoding cytosolic AGPase |
cause |
reduction in total endosperm starch |
Zea mays; Hordeum vulgare; Oryza sativa |
| Classical mutations at shrunken2 (sh2) locus |
led to identification and cloning of |
genes encoding cytosolic AGPase large subunit (LS) |
Zea mays |
| mutant and nonmutant sibling kernels on the same ear |
ruled out the possibility that reduced endosperm starch resulted from |
compromised metabolism in the parent plant |
Zea mays |
| antisense inhibition of SBEI and SBEIIb in TRS |
causes significantly reduced expression of |
SBEIIa |
|
| multisubunit complexes of starch biosynthetic enzymes |
is necessary for |
SBE function |
|
| loss of debranching enzyme (DBE) of the (ARA1, ATISA1, ISA1, AT4G16130) class |
causes |
dramatic phenotype |
|
| ss2ss3isa mutant |
is very similar to |
ss2isa mutant |
Arabidopsis thaliana |
| branch point distribution |
is altered in |
isa mutants |
Arabidopsis thaliana |
| glucans from (AtSS2, SS2, AT3G01180) mutants |
have fewer chains of |
DP 11 to 25 |
Arabidopsis thaliana |
| differential expression of starch synthase (SS), branching enzyme (BE), and isoamylase (ISA) |
determines whether |
starch (in leaves) or glycogen (in specialized myrmecophytic Müllerian bodies) is made |
Cecropia peltata |
| AGPLLZM and BT2a |
coexpression fails to yield |
functional AGPase activity |
Zea mays; Escherichia coli |
| Agpl3 gene |
remains as a potential contributor to AGPase large subunit in |
embryo tissue |
Zea mays |
| dark bands |
correspond to |
endogenous starch synthase activities (ATSS1, SS1, AT5G24300) and (SS3, AT1G74000) |
Arabidopsis thaliana |
| glucan elongating activity and specificity of YFP-SS4C |
may differ from |
that of YFP-GS |
|
| Arabidopsis (ATSS1, SS1, AT5G24300) and (AtSS2, SS2, AT3G01180) expressed in yeast |
were less efficient in glucan synthesis than |
(SS3, AT1G74000) and (ATSS4, SS4, SSIV, AT4G18240) |
Arabidopsis thaliana; Saccharomyces cerevisiae |
| different molecular structures in TRS heterogeneous starch granules |
may originate from |
different dosages of three SBE isoforms |
|
| SSI and SSIIa |
display similar deposition mode |
SBEs in TQ and TRS |
|
| phytoglycogen |
generally has fewer chains between DP 8 and 17 compared to |
insoluble glucans from a given ssisa combination |
Arabidopsis thaliana |
| soluble polysaccharides in sugary2 (SSIIa-deficient) maize |
were quantified and reported to have |
unchanged levels relative to wild type |
Zea mays |
| Null mutations in genes encoding cytosolic AGPase |
do not cause |
complete starch deficiency |
Zea mays; Hordeum vulgare; Oryza sativa |
| agpllzm-Ds1 homozygous mutant |
fails to accumulate |
AGPLLZM transcript |
Zea mays |
| agpsemzm-Mu1 homozygous mutant embryo |
shows markedly less intense |
iodine staining |
Zea mays |
| agpllzm loss of function |
caused major reductions in |
AGPase activity |
Zea mays |
| reverse reaction catalyzed by plastidial AGPase |
generates |
Glc-1-P |
Zea mays |
| corresponding maize mutant |
is characterized by |
severely reduced starch content in endosperm |
Zea mays |
| epistatic interactions of Rc and (ATIPT5, IPT5, AT5G19040) |
were found in |
starch biosynthesis gene encoding granule-bound starch synthase II |
|
| ISA1-ISA2 involvement in glucan turnover |
is indicated by |
fact that ss2ss3isa triple mutant has high glucan levels |
Arabidopsis thaliana |
| expression of ISA1-ISA2 enzyme in Escherichia coli |
did not promote |
synthesis of more amylopectin-like polymer |
Escherichia coli |
| plastidial AGPase activity |
contributes to |
starch production in endosperm |
Zea mays |
| agplemzm locus |
appears to be the major contributor of AGPase large subunit in |
embryo |
Zea mays |
| (ATSS4, SS4, SSIV, AT4G18240) mutant |
had |
pale and starch-free young leaves |
Arabidopsis thaliana |
| transgenic potato tubers expressing Ec GS |
produced |
less starch, which was more branched, and accumulated more soluble sugars |
Solanum tuberosum |
| three SBE isoforms in four heterogeneous starches |
show gradually decreasing trend from polygonal to hollow starch granules |
SBE abundance gradient |
|
| ISA (isoamylase) activity |
can suppress |
glucan accumulation |
|
| complementation of adenosine 5-diphosphate-glucose pyrophosphorylase (AGPase)-deficient Arabidopsis mutant with mutagenized redox-insensitive AGPase |
resulted in |
maintenance of slightly higher starch levels throughout the diel cycle in long day or high light regimes |
Arabidopsis thaliana |
| AGPLLZM |
null mutations in |
agpllzm mutant |
Zea mays |
| Genes apgsemzm and agpllzm |
were each required for |
production of approximately 7% of total endosperm starch |
Zea mays |
| granule-bound starch synthase I (GBSSI) |
is buried in |
starch granule |
|
| regular alterations of molecular structures in four TRS heterogeneous starch granules |
are reminiscent of |
effects of different SBEIIb dosages on molecular structures |
|
| three SBEs in TRS |
exhibit accumulating trend in granule-bound fraction before 10 DAF |
granule-bound SBE accumulation in TRS |
|
| ss2ss3 mutant |
shows no starch granules by TEM; only |
phytoglycogen or intermediate particles |
Arabidopsis thaliana |
| ADPGlc formation |
is |
important metabolic control point |
|
| 14-3-3 protein isoform (A0A1D6CLX4) |
is significantly downregulated at |
20 days after anthesis (DAA) |
Triticum aestivum |
| SSI |
is component of |
phosphorylation-dependent protein complex |
|
| TF modules from WGCNA |
were linked to |
correlation coefficients of starch metabolic gene clusters of TF–SSG pairs |
Zea mays |
| these miRNAs |
may be involved in |
regulation of the initiation of starch accumulation |
Zea mays |
| starch-filling in mn1 endosperm |
is relatively more rapid than |
starch-filling in Mn1 endosperm |
Zea mays |
| transport function of Brittle1 (BT1) |
was directly assessed after |
expression in E. coli and reconstitution into liposomes |
Zea mays; Escherichia coli |
| reduction of tomato pollen viability by heat |
relates to |
decreased starch accumulation in developing pollen grains |
Solanum lycopersicum |
| Rc and (ATIPT5, IPT5, AT5G19040) haplotype 4-enriched rice lines |
had |
elevated amylose content (AM1) |
|
| sucrose signal transmission by TREHALOSE-6-PHOSPHATE (T6P) |
triggers |
activation of ADP-glucose pyrophosphorylase (AGPase) |
Arabidopsis thaliana |
| phosphorylation |
catalyzes the formation of |
LMW complexes from monomers of SSI, SSIIa and SBEIIb |
Zea mays |
| five phospho-amino acid residues in wheat granule-bound SSIIa (Ta-SSIIa) |
were identified with varying degrees of conservation in |
other plant species |
Triticum aestivum |
| ventral endosperm of osnf-yb9 seeds |
was filled with |
loosely packed, spherical starch granules with large air spaces |
Oryza sativa |
| OtsA overexpression in Arabidopsis |
causes |
activation of ADP-glucose pyrophosphorylase (AGPase) |
Arabidopsis thaliana |
| AGPL1 and AGPS1 |
are involved in the promotion of |
starch biosynthesis |
Solanum lycopersicum |
| starch biosynthesis in tomato fruit |
is mainly regulated by |
sugar in an ABA- and osmotic stress-independent manner |
Solanum lycopersicum |
| reduction in SBE IIa and SBE IIb expression |
causes significant reduction in |
frequency of branches in amylopectin |
Hordeum vulgare |
| ADP-glucose (ADP-Glc) |
is exclusively generated in |
cytosol |
Zea mays |
| repression or loss of (NTT, WIP2, AT3G57670) activity |
results in dramatically decreased |
starch levels in potato tubers |
Solanum tuberosum |
| Rc and (ATIPT5, IPT5, AT5G19040) |
verified the role of these genomic loci in influencing |
amylose levels |
|
| Ery4P (erythrose-4-phosphate) |
is potent inhibitor of |
phosphoglucoisomerase |
|
| phosphorylation |
enhanced |
catalytic activity of SBEIIb |
Zea mays |
| overexpression of ZmMYB138 |
had significant effect on |
activities of the promoters of (ATBT2, BT2, AT3G48360) and Ae1 |
Zea mays |
| OsBEIIb |
is involved in |
starch biosynthesis |
Oryza sativa |
| gene editing technologies |
can be used to |
modify other starch traits |
|
| SBEIIb |
is phosphorylated |
SBEIIb phosphorylation |
|
| dissociation constants (Kd) of SSIIa for amylopectin |
are identical under |
phosphorylating (ATP treatment) and dephosphorylating (APase treatment) conditions |
|
| 49 SSGs |
were identified and expressed in |
at least one sequenced sample |
Zea mays |
| RBE1 |
is involved in |
starch biosynthesis |
Oryza sativa |
| (GPT, AT2G41490) function impairment due to antisense repression |
caused |
significant reduction in starch content of embryo |
Vicia |
| ATP treatment (phosphorylation) |
enhanced |
catalytic activity of immunopurified maize SSIIa |
Zea mays |
| SSIIa |
co-migrated with SSI and SBEIIb in |
two different positions following electrophoresis of ATP-treated amyloplasts |
Zea mays |
| ZmMYB138 |
failed to interact with |
promoters of Sh2, (ATBT2, BT2, AT3G48360) and Ae1 genes |
Zea mays |
| SPK knockdown |
resulted in extreme reduction in protein accumulation of |
RBE1 |
Oryza sativa |
| OsSSI |
expression was suppressed in |
osnf-yb9 mutants |
Oryza sativa |
| starch synthase (SS) activity biased toward short external chains |
promotes |
phytoglycogen biosynthesis |
|
| ADP-Glc in ss1ss2ss3 mutants |
is 35 times elevated |
compared to wild type |
Arabidopsis thaliana |
| Cecropia peltata |
can make both |
starch (in leaves) and glycogen (in specialized myrmecophytic Müllerian bodies) |
Cecropia peltata |
| Cytosolic and plastidial AGPases |
both contribute to |
production of endosperm starch |
|
| amylose and amylopectin synthesis |
is not significantly different between |
TQ and TRS at 4 DAF |
|
| SBEs in TRS following 10 DAF stage |
seem to be maintained at stable level in granule |
granule-bound SBE level |
|
| lowly expressed SSGs |
showed |
hypermethylation of their coding regions |
Zea mays |
| genes conferring lower glycemic index (GI) |
go beyond |
amylose |
|
| phosphorylated SSIIa |
occurs in vivo as well as in vitro |
phosphorylation phenomenon |
|
| dephosphorylation |
decreased |
catalytic activities of SBEIIb |
Triticum aestivum |
| HECs |
have been previously identified in |
endosperms of wild-type maize and ae-mutants |
Zea mays |
| phosphorylation state of each component within the complex |
could result in |
altered electrophoretic migration and catalytic activity of the protein |
Zea mays |
| recombinant maize SSIIa |
exhibited comparatively higher Vmax for amylopectin than |
Vmax observed for stromal maize SSIIa |
Zea mays |
| Group 3 |
contained |
17 genes including Ss2.3, (BT1, AT5G63160) and Sbe3 |
Zea mays |
| coordinated regulatory network of starch biosynthesis |
was established |
maize endosperm |
Zea mays |
| salinity stress |
up-regulates |
AgpL1 expression |
Solanum lycopersicum |
| Arabidopsis |
is used as model for |
starch biosynthesis in Chloroplastida |
Arabidopsis thaliana |
| (ATSS1, SS1, AT5G24300) and (SS3, AT1G74000) |
may indicate a synergistic relationship between |
starch content reduction in ss1-ss3 double mutant |
|
| (SS3, AT1G74000) or (AtSS2, SS2, AT3G01180) alone |
is the primary determinant of |
amylopectin chain length distribution |
|
| Brittle1 (BT1) |
involvement in ADP-Glc transport has been |
postulated |
Zea mays |
| SSIIa |
is responsible for starch granule association of |
SSI and SBEIIb |
|
| Ta-Tyr 268 and Ta-Ser 776 |
are conserved in |
cereals but not in Arabidopsis |
Triticum aestivum; Oryza sativa; Zea mays; Arabidopsis thaliana |
| dorsal endosperm of osnf-yb9 seeds |
consisted of |
densely packed, irregularly polyhedral starch granules |
Oryza sativa |
| starch biosynthesis |
is limited by knowledge of |
genetic approaches to modify starch in crops |
|
| sugar precursors |
undergo major tissue-dependent change in abundance during |
wheat grain development |
Triticum aestivum |
| starch biosynthesis proteins in embryo |
show a similar pattern |
gradual increase from 12 to 26 days after anthesis (DAA) |
Triticum aestivum |
| SSIIa |
is limited by |
availability of glucan branching |
Zea mays |
| heteromeric enzyme complexes |
are stable |
stability of heteromeric complexes |
Zea mays |
| samples from 3 to 7 DAP and 20–25 DAP |
lacked |
transcriptional activity |
Zea mays |
| starch |
is synthesized from |
carbon skeletons derived from the Calvin–Benson–Bassham cycle |
Oryza sativa |
| OsSSIVb |
is involved in |
starch biosynthesis |
Oryza sativa |
| OsBEI |
was remarkably activated in |
osnf-yb9 mutants |
Oryza sativa |
| SBEIIb |
is phosphorylated in |
phosphorylation-dependent protein complex |
|
| SSIIa |
is phosphorylated in |
wheat endosperm amyloplasts |
Triticum aestivum |
| SSIIa |
was immunopurified and shown to be substantially free from contamination by |
other starch synthases |
|
| two recombinant isoforms of maize endosperm-specific 14-3-3 (ZmGF-14-6 and ZmGF14-4) |
were able to interact with |
GBSSII, SBEIIb and ISA |
Zea mays |
| clustering of the unmonitored SSGs that showed obvious expression within 7 and 25 DAP |
yielded |
map depicting the spatial expression of the SSGs |
Zea mays |
| small subunit of endosperm AGPase |
influences |
starch content in maize endosperm |
Zea mays |
| new information about starch biosynthesis |
could be exploited to |
create novel variability in carbohydrate polymers |
|
| novel variability in carbohydrate polymers |
is created in |
cereal grains |
|
| sucrose synthase (SUS) |
is highly expressed in |
storage organs |
|
| protein factors |
differentially affect |
sucrose synthase (SUS) activity in sucrose synthesis and sucrose cleavage |
|
| developing zpu1-204 endosperm |
accumulates |
branched malto-oligosaccharides |
Zea mays |
| Iso1 (isoamylase)/pullulanase-defective ( (AtBE2, ATISA2, BE2, DBE1, ISA2, AT1G03310) (ATLDA, ATPU1, LDA, PU1, AT5G04360) double-mutant) lines |
display |
92% decrease in starch content |
Arabidopsis thaliana |
| starch synthase IIa (SSIIa) |
interacts with |
starch phosphorylase (SP) |
Zea mays |
| dephosphorylation |
decreased |
catalytic activities of SBEIIa |
Triticum aestivum |
| phosphorylation |
might actually dissociate |
HMW complexes containing maize SSIIa |
Zea mays |
| glucose |
undergoes polymerization to form |
starch granules |
|
| (ATFD1, FD1, AT1G10960) mutant chloroplasts |
have no visible |
starch granules |
Oryza sativa |
| SSIIa phosphorylation |
affects |
Vmax |
Zea mays |
| protein phosphorylation |
promotes dissociation of |
SSIIa-containing heteromeric enzyme complexes |
Zea mays |
| ATP treatment |
enhanced interactions between |
SSIIa and SBEIIb |
Zea mays |
| ZmMYB115 |
interacted with |
promoters of the Du1 and Wx genes |
Zea mays |
| 13–25 DAP endosperms |
are |
periods during which starch accumulates rapidly in endosperm |
Zea mays |
| starch granules in SO chloroplasts |
were fewer in |
SO compared to SY |
|
| osnf-yb9 seeds |
had significant reductions in |
total starch content |
Oryza sativa |
| OsAGPS2b |
expression levels were increased in |
osnf-yb9 mutants |
Oryza sativa |
| TubZIP28 |
shares identical function with |
TabZIP28 |
Triticum urartu; Triticum aestivum |
| SBEIIb |
remains associated with |
immunopurified SSIIa |
Zea mays |
| formation of different SSIIa-containing HECs |
could result in |
altered electrophoretic migration and catalytic activity of the protein |
Zea mays |
| phosphorylation of each site |
is not clear whether it leads to |
the assembly or disassembly of different protein complexes |
Zea mays |
| transcription factor |
regulates |
starch synthesis gene expression |
Zea mays |
| bHLH144, OsNF-YB1 and OsNF-YC12 |
directly regulate the expression of |
Waxy (Wx) |
Oryza sativa |
| OsSSIIIa |
is involved in |
starch biosynthesis |
Oryza sativa |
| band E |
identified as |
SSIIa |
Zea mays |
| isoamylase (ISA, EC 3.2.1.68) |
is |
starch debranching enzyme |
|
| short branches with DP2-4 |
are absent in |
non-treated amylopectin |
Oryza sativa |
| starch biosynthesis control |
involves intimate interplay of |
multiple wheat protein isoforms involved in starch synthesis and sugar precursors |
Triticum aestivum |
| alteration of protein phosphorylation states |
affects |
partitioning of heteromeric complexes |
Zea mays |
| key starch branching enzyme SBE2b |
determines |
formation of amylopectin |
Zea mays |
| transcriptional regulation of SSGs by ZmMYB138 and ZmMYB115 |
combined with |
Zma-miR159k-3p can indirectly regulate starch biosynthesis in maize endosperm by regulating the expression of ZmMYBs |
Zea mays |
| Su1 (GRMZM2G138060) |
showed |
strong negative correlation between mRNA signals and DNA methylation marks |
Zea mays |
| (AtSS2, SS2, AT3G01180) |
is phosphorylated in |
Arabidopsis chloroplasts |
Arabidopsis thaliana |
| annotated starch biosynthesis-related genes |
were extracted from |
maize |
Zea mays |
| SSIIa |
interacts with |
SBEIIb |
|
| (MIR167C, AT3G04765) |
was identified to regulate |
starch synthesis-related TFs |
Zea mays |
| starch |
is synthesized exclusively in |
plastids |
|
| SBE IIa-/SBE IIb- line |
may require |
SBE I to act on both amylopectin and amylose |
Hordeum vulgare |
| accumulation of starch biosynthesis proteins in endosperm |
is most pronounced |
in endosperm |
Triticum aestivum |
| SSIIa |
undergoes post-translational modification |
phosphorylation |
Zea mays |
| ATP treatment of SSIIa alone |
did not affect |
catalytic activity of SSIIa |
|
| enzymes and enzyme isoforms |
contribute to production of |
amylopectin and amylose |
|
| protein kinase inhibitors |
affects |
protein phosphorylation |
Zea mays |
| protein phosphorylation |
promotes association of |
SSIIa-containing heteromeric enzyme complexes |
Zea mays |
| Km values of immunopurified, untreated, stromal maize SSIIa |
were comparable to |
those previously determined for recombinant maize SSIIa |
Zea mays |
| miRNA–TF–SSG regulatory network |
was predicted in |
maize starch biosynthesis |
Zea mays |
| poor response to salinity-stress |
supports |
ABA-independent regulation of starch biosynthesis |
Solanum lycopersicum |
| starch branching enzyme (SBE) IIa |
plays distinct role in determining |
fine structure of amylopectin |
Hordeum vulgare |
| synthesis of low mass fraction of starch |
is exclusively under the control of |
(GBSS1, AT1G32900) activity |
|
| starch biosynthesis under salinity stress |
is ABA-independent |
abscisic acid (ABA) signaling |
Solanum lycopersicum |
| mutations abolishing expression of SBE IIa in maize |
led to no significant change in |
endosperm amylose content or starch structure |
Zea mays |
| 14-3-3 proteins |
is reduced dramatically after |
15 days after anthesis (DAA) |
Triticum aestivum |
| incubation of maize amyloplast stroma with tetra-sodium pyrophosphate |
resulted in |
electrophoretic mobility shift |
Zea mays |
| yellow and red modules |
may be mainly regulated by |
SSG cluster 3 |
Zea mays |
| AGPL1 |
is not up-regulated by |
abscisic acid (ABA) |
Solanum lycopersicum |
| ABA |
affects |
AgpL3 expression |
Solanum lycopersicum |
| starch granules |
are composed of |
amylose and amylopectin |
|
| (SBE2.2, AT5G03650) |
is involved in |
formation of starch granules |
|
| lts1 mutants |
accumulated |
many starch granules |
Chlamydomonas reinhardtii |
| salinity stress |
enhances carbohydrate accumulation as |
starch |
Solanum lycopersicum |
| AGPL2 |
encodes |
large subunit of AGPase |
Solanum lycopersicum |
| mannitol |
is not effective as single effector for |
AgpL3 expression |
Solanum lycopersicum |
| AGPL1 |
is expressed in a co-ordinated manner to |
enzymatic activity and starch accumulation pattern during fruit development |
Solanum lycopersicum |
| amylose |
is |
component of starch granules |
|
| BEII class |
is represented by |
two different enzymes |
|
| mutations in the gene encoding BEI |
have no notable effect on |
endosperm starch |
Zea mays |
| granule-bound starch synthase (GBSS) |
is identified in starch granules of |
barley starch granules |
Hordeum vulgare |
| Arabidopsis cyFBPase knock-out plants |
exhibit |
major starch accumulation with wild-type phenotype |
Arabidopsis thaliana |
| rice floury-2 mutant |
has pleiotropic effects on |
PUL activity |
Oryza sativa |
| crystallinity of starch granules in PUL mutant lines |
were almost the same as |
crystallinity of starch granules in wild type |
Oryza sativa |
| high activities of ISA and PUL |
were observed at |
early-to-middle stages and middle-to-late stages respectively during rice endosperm development |
Oryza sativa |
| phosphorylation (ATP treatment) |
caused substantial alterations in |
electrophoretic mobility of amyloplast SSIIa |
Zea mays |
| protein phosphorylation |
enhances |
catalytic activity of SSIIa |
Zea mays |
| Cluster 4 |
is regulated by |
green and black modules |
Zea mays |
| overexpression of ZmMYB115 |
significantly suppressed |
Du1 promoter activity |
Zea mays |
| pyruvate phosphate dikinase (PPDK, AT4G15530) |
has an essential role in |
starch synthesis and energy supply during grain development |
Triticum aestivum |
| ATP-dependent stimulation of SSIIa activity |
is associated with |
interaction with SBEIIb |
Zea mays |
| IbPMA1 overexpression and IbbHLH49 overexpression |
jointly increase |
tuber starch yield |
Ipomoea batatas |
| AGPS1 |
is up-regulated in |
developing fruits |
Solanum lycopersicum |
| AGPL1 |
is likely to be responsible for |
enhanced starch biosynthesis under salinity stress |
Solanum lycopersicum |
| α(1→6) linkage formation |
is important to |
fine structure of amylopectin and amylose |
Hordeum vulgare |
| down-regulation of individual enzymes involved in starch biosynthesis |
is difficult to predict effects of due to |
pleiotrophic effects on protein complex formation |
Hordeum vulgare |
| sucrose and mannitol |
affect |
AgpL3 expression |
Solanum lycopersicum |
| plastidic Glc6P/phosphate translocator (GPT) |
mediates import of |
glucose 6-phosphate (Glc6P) |
|
| up-regulation of Soluble starch synthase1 ( (ATSS1, SS1, AT5G24300) ) |
is an incongruity compared with |
other starch biosynthesis genes |
Arabidopsis thaliana |
| suppression of SSI protein |
changes |
crystalline order in starch granules |
Triticum aestivum |
| starch in cereal grain |
is produced in |
amyloplasts |
Triticum aestivum |
| ss3a/be2b mutant |
shows slightly lower |
SSI activity level |
Oryza sativa |
| amylose |
is contained in |
fraction I (Fr. I) |
Oryza sativa |
| SSIIIa deficiency |
increases |
ADP-glucose concentration in amyloplast |
Oryza sativa |
| conversion of glucose-6-phosphate to glucose-1-phosphate by pPGM |
is essential for |
starch synthesis in the light |
Arabidopsis thaliana |
| SSI–BEIIa |
is common among |
wheat, maize, and rice |
Triticum aestivum; Zea mays; Oryza sativa |
| BN-PAGE |
provided |
new and useful perspective to analysis of starch biosynthetic enzyme complexes |
Oryza sativa |
| starch phosphorylase |
undergoes major tissue-dependent change in abundance during |
wheat grain development |
Triticum aestivum |
| ZmMYB115 (GRMZM2G423833) |
expression levels correlated with |
expression of SSGs (ATSS1, SS1, AT5G24300) Du1, Wx, etc |
Zea mays |
| soluble starch synthase (SS) family |
is involved in |
starch biosynthesis |
Oryza sativa |
| IbbHLH49 overexpression |
increases |
tuber starch yield |
Ipomoea batatas |
| mannitol and ABA |
do not up-regulate |
AgpS1 expression |
Solanum lycopersicum |
| salinity stress |
promotes |
AGPase activity in developing tomato fruit |
Solanum lycopersicum |
| abolishing SBE I activity |
resulted in no major impact on |
starch properties |
|
| SS activity |
affects |
net result of branch placement accomplished by balanced activities of starch branching enzymes and starch debranching enzymes |
Arabidopsis thaliana |
| starch debranching enzyme (DBE) genes |
are conserved in |
Chloroplastida |
|
| AGPL1 |
is up-regulated by |
sugar |
Solanum lycopersicum |
| changes in starch structure due to altered expression of branching enzymes |
is indicated by changes in |
starch swelling power, thermal, and pasting properties |
Hordeum vulgare |
| SBE IIa |
is important in controlling |
amylose content in wheat |
Triticum aestivum |
| (ATSS4, SS4, SSIV, AT4G18240) |
is important factor in |
granule initiation |
Arabidopsis thaliana |
| salinity stress |
promotes and extends |
starch accumulation during early fruit development |
Solanum lycopersicum |
| AGPL3 |
does not participate in |
starch accumulation in fruit |
Solanum lycopersicum |
| elimination or reduction of SBE I expression |
did not yield noticeable phenotype in |
wheat |
Triticum aestivum |
| OCL1 overexpression line K6 |
showed absence of |
starch accumulation in bundle sheath cells |
Zea mays |
| GBSSI gene in P. capillare |
produces |
functional protein that synthesizes endosperm amylose |
Panicum capillare |
| Brachypodium starch granules |
show more flat shapes |
granule morphology |
Brachypodium |
| be2b mutant (EM10) |
crossed with |
ss3a mutant (e1) |
Oryza sativa |
| significant differences in combinations of isozyme interactions |
were revealed among |
cereals |
Triticum aestivum; Zea mays; Oryza sativa |
| absence of SBE IIb |
allows |
SBE I to be active in branching long-chained starch molecules |
Hordeum vulgare |
| plastidic nucleotide transporter (NTT) and plastidic Glc6P/phosphate translocator (GPT) |
co-limit |
starch biosynthesis |
Solanum tuberosum |
| zymogram |
uses |
ADP-glucose |
|
| sucrose and ABA |
up-regulate |
AgpL1 expression |
Solanum lycopersicum |
| possibility that salinity stress promotes phyto-assimilation on the fruit surface |
was ruled out because |
starch granules appeared only in inner pericarp, columella, and placenta tissue |
Solanum lycopersicum |
| starch branching enzyme (SBE) IIa |
plays role in determining |
distribution of chain lengths |
Hordeum vulgare |
| amylose extender mutants of maize |
result from |
lesions eliminating expression of SBE IIb gene |
Zea mays |
| wheat lines with significant reduction in SBE IIa expression |
showed concomitant reduction in |
SBE IIb |
Triticum aestivum |
| Arabidopsis lines containing only (AtSS2, SS2, AT3G01180) and (ATSS4, SS4, SSIV, AT4G18240) |
have |
strong reduction in starch content |
Arabidopsis thaliana |
| IbPMA1 overexpression |
increases |
tuber starch yield |
Ipomoea batatas |
| lts1 mutant strains |
contain |
starch granules |
|
| salinity stress |
up-regulates |
AgpS1 expression |
Solanum lycopersicum |
| low mass fraction in (AtSS2, SS2, AT3G01180) mutant |
is strictly under the control of |
(GBSS1, AT1G32900) |
|
| granule-bound SSI isoforms |
levels increase gradually from |
12 to 26 days after anthesis (DAA) |
Triticum aestivum |
| formation of pyruvate |
may be beneficial to |
synthesis at the grain filling stage |
Triticum aestivum |
| electrophoretic mobilities of SSIIa bands |
are markedly altered by |
conditions affecting protein phosphorylation |
Zea mays |
| SBEIIb bands |
were coincident with |
SSIIa bands c and d in ATP-treated samples |
Zea mays |
| ATP treatment of amyloplasts |
enhanced |
interaction between SSIIa and SBEIIb |
Zea mays |
| overexpression of ZmMYB115 |
enhanced |
Wx promoter activity |
Zea mays |
| starch synthase (SS) gene classes |
are conserved in |
chloroplast-containing organisms |
|
| amylose |
is composed of |
α-D-glucosyl units |
|
| starch synthase (SS) |
catalyzes |
starch polymer formation |
|
| su2 gene |
codes for |
SSIIa |
Zea mays |
| rate of starch accumulation by grains |
was significantly and positively correlated with |
ABA content |
|
| low PUL activity |
is not the cause of |
reduction in the amount of iodine-stained starch |
Oryza sativa |
| ADP-glucose (ADP-Glc) pyrophosphorylase (AGPase) |
catalyzes formation of |
ADP-glucose (ADP-Glc) |
|
| ae– mutant |
is replaced by novel protein–protein interactions consisting of |
starch synthase I (SSI), starch synthase IIa (SSIIa), starch branching enzyme I (SBEI), starch branching enzyme IIa (SBEIIa), and starch phosphorylase (SP) |
Zea mays L. |
| novel protein–protein interactions on starch granule structure in ae– maize |
will be discussed |
consequences of novel protein–protein interactions |
Zea mays L. |
| bamboo meristem bud |
accumulates |
plenty of starch after the cool autumn and cold winter |
bamboo |
| altered SBE activity |
has primary effect on |
distribution or frequency of branches |
Hordeum vulgare |
| duplication of starch biosynthetic enzyme isoforms in monocots |
allows |
differential expression in tissue and developmental stages |
|
| metabolic changes in starch biosynthesis |
did not occur in |
(CYL1, NAGLU, AT5G13690) seeds |
|
| branching enzyme IIb (BEIIb) |
is identified in starch granules of |
rice starch granules |
Oryza sativa |
| PUL function |
partially overlaps with |
(ARA1, ATISA1, ISA1, AT4G16130) function |
Oryza sativa |
| each starch synthase, branching enzyme, and debranching enzyme isoform |
has |
varied affinity for different glucan substrates |
|
| various protein–protein interactions in ae– mutant |
are reflected in |
complement of starch synthesizing enzymes detected in starch granules as granule-associated proteins |
Zea mays L. |
| (GBSS1, AT1G32900) |
appears to be increased in activity compared to normal when |
(AtSS2, SS2, AT3G01180) and (ATSS1, SS1, AT5G24300) are missing |
|
| starch synthase IIa (SSIIa) |
is identified in starch granules of as |
wheat starch granules |
Triticum aestivum |
| integrated cluster structure of amylopectin |
is maintained during |
starch biosynthesis |
Oryza sativa |
| stromal protein complexes |
are mirrored in |
complement of starch synthesizing enzymes detected in starch granules |
Zea mays |
| starch biosynthesis proteins |
levels increase gradually in endosperm from |
12 to 26 days after anthesis (DAA) |
Triticum aestivum |
| AGPase, GBSSI, SSIIa, SBEI, SBEIIa, SBEIIb, (ATPHO1, PHO1, AT3G23430) (AtBE2, ATISA2, BE2, DBE1, ISA2, AT1G03310) and (BT1, AT5G63160) |
are phosphorylated |
phosphorylation |
|
| starch biosynthetic enzymes |
function through association in |
heteromeric enzyme complexes (HECs) |
|
| Cluster 2 |
is regulated by |
green and gray modules |
Zea mays |
| SS activity |
affects |
chain elongation |
Arabidopsis thaliana |
| (SS3, AT1G74000) |
is necessary for |
generation of linked clusters necessary for substantial granule growth |
Arabidopsis thaliana |
| sh1 mutant |
significantly decreased |
level of starch in seed |
Zea mays |
| sucrose synthase (SUS) |
is highly expressed in |
fruits |
|
| chain-length distribution changes |
were not observed in |
zpu1-204 endosperm |
Zea mays |
| protein phosphorylation |
is emerging as potentially important mechanism for control of |
starch biosynthesis |
|
| starch branching enzyme IIb (SBEIIb) |
is |
most abundant protein in amyloplast stroma |
Zea mays L. |
| soluble starch synthase |
undergoes major tissue-dependent change in abundance during |
wheat grain development |
Triticum aestivum |
| sucrose synthase (SuSy) |
acts as |
first enzyme in the conversion of sucrose to starch |
Triticum aestivum |
| starch synthesis proteins |
increase in their levels gradually from |
12 to 26 days after anthesis (DAA) |
Triticum aestivum |
| SSIIa phosphorylation |
has minor effects on |
substrate Km |
Zea mays |
| endosperm |
stores |
starch |
|
| miR169o |
was identified to regulate |
starch synthesis-related TFs |
Zea mays |
| OsNF-YC12 knockout |
influences |
starch granule formation |
Oryza sativa |
| OsSSI |
is involved in |
starch biosynthesis |
Oryza sativa |
| OsAGPL2 |
is involved in |
starch biosynthesis |
Oryza sativa |
| suppression of SSIIa or SBEIIa expression in mutants |
cause more severe changes in |
starch granule structure |
Pisum sativum; Zea mays; Triticum aestivum; Hordeum vulgare |
| distinct features |
could be linked to |
specific differences in starch biosynthesis enzyme expression profiles |
Brachypodium distachyon |
| be2b mutant |
shows greatly reduced |
starch content |
Oryza sativa |
| (ATPHO1, PHO1, AT3G23430) |
elutes in |
narrow molecular weight range |
Oryza sativa |
| BEI–PUL interaction |
is unexpected |
finding |
Oryza sativa |
| ADP-glucose pyrophosphorylase (AGPase, EC 2.7.7.27) |
catalyses |
synthesis of ADP-glucose from glucose-1-phosphate and ATP |
Solanum lycopersicum |
| ADP-glucose pyrophosphorylase |
regulates |
starch content |
|
| (ATSS4, SS4, SSIV, AT4G18240) |
is involved exclusively in |
granule initiation |
|
| suppression of SBE IIb in maize |
was required in order to obtain |
high amylose phenotype |
Zea mays |
| granule proteome analysis |
is used as means of investigating |
functional interactions among starch biosynthetic enzymes |
|
| PUL |
has a distinct role in |
amylopectin biosynthesis |
Zea mays; Oryza sativa |
| wild type maize |
exhibits single migration form of |
starch branching enzyme (BE) BEIIb |
Zea mays |
| double mutants between PUL-null and mild sug1 mutants |
have amounts of WSP and short chains (DP ≤7) of amylopectin higher than |
sug1 mutant |
Oryza sativa |
| ISA and PUL |
are involved in |
construction of the amylopectin fine structure |
Oryza sativa |
| ISA (isoamylase) |
presumed function is |
trimming of improper branches of amylopectin |
Oryza sativa |
| starch synthase I (SSI) |
interacts with |
starch branching enzyme IIb (SBEIIb) |
Zea mays |
| starch phosphorylase (SP) |
is phosphorylated within |
mutant protein complex from ae endosperm |
Zea mays |
| fruit sink capacity |
can be controlled by |
modification of AGPase gene expression |
Solanum lycopersicum |
| SBE IIb reduced line |
shows no change in |
amylose content |
Hordeum vulgare |
| starch debranching enzymes (DBE) |
catalyzes |
hydrolysis of branch linkages |
|
| (ATSS1, SS1, AT5G24300) |
is necessary to restrict |
abundance of chains in range of approximately DP 15-22 |
|
| disruption of OsSUT1 |
did not affect |
starch accumulation during pollen development |
Oryza sativa |
| soluble starch synthase II (AtSS2, SS2, AT3G01180) |
expression was markedly downregulated in |
floral nectary of NtCOI1-silenced tobacco at S9 |
Nicotiana tabacum |
| SSI-RNAi suppression |
leads to |
low level of enzymatic activity for starch synthase I (SSI) |
Triticum aestivum |
| Branching enzyme (BE) IIb |
is |
major BE isozyme |
Oryza sativa |
| BEIIb deficiency |
induces more pronounced phenotype than |
BEI or BEIIa deficiency |
Oryza sativa |
| AAC (%) of other lines |
is still low (<10%) at |
10 DAF |
Oryza sativa |
| BEIIb deficiency |
increases |
ADP-glucose concentration in amyloplast |
Oryza sativa |
| protein–protein complexes of SSI, SSIIa, and BEIIb |
are trapped in |
starch granules during starch biosynthesis |
Zea mays |
| isozyme-specific antibodies |
enabled discovery of |
active, high molecular weight, starch biosynthetic enzyme complexes in rice endosperm |
Oryza sativa |
| PUL |
was co-eluted in fractions corresponding to |
mass >700kDa |
Oryza sativa |
| rice SSIIa |
was not found in |
monomeric size |
Oryza sativa |
| pgm1pgm2 lines 1 and 2 |
lacked starch in |
root tips |
Populus tremula × tremuloides |
| eliminating starch synthase (SS) SSIIa |
does not affect |
granule-bound starch synthase (GBSSI) abundance |
Zea mays |
| starch branching enzyme (BE) |
catalyzes |
starch polymer formation |
|
| starch synthase IIa (SSIIa) |
is identified in starch granules of as |
wheat starch granules |
Triticum aestivum |
| sugary1 mutants |
accumulate |
highly branched phytoglycogen |
Zea mays |
| starch phosphorylase (SP) |
provides functional complementation for loss of |
starch branching enzyme IIb (SBEIIb) |
Zea mays |
| starch |
comprises |
large lenticular A-type granules and smaller near-spherical B-type granules |
Triticeae |
| GBSS |
previously detected in |
maize starch granules |
Zea mays |
| isoamylase (ISA, EC 3.2.1.68) |
can debranch |
amylopectin |
|
| PUL gene |
is present in |
potato |
Solanum tuberosum |
| zpu1-204 mutant |
not found in |
wild type |
Zea mays |