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rna silencing

11921 relationships annotated with this phrase. Showing first 500 of 11921.
Source entity Relationship Target entity Species
double-stranded RNA molecule triggers posttranscriptional silencing of corresponding gene Solanum lycopersicum
RNAi-induced silencing complexes contain proteins encoded by ARGONAUTE gene family
VmR2 -siR1 targets F-box/LRR-repeat protein 14 isoform X2 (MdLRP14) Malus domestica
Garnelo Gómez team studied RNA silencing and links to receptor kinases and virus spreading
single strands of siRNA duplexes are incorporated into RNAi-induced silencing complexes
(MIR164, MIR164A, AT2G47585) and (MIR164, MIR164B, AT5G01747) combined expression was reduced in anti-164bsl plants Arabidopsis thaliana
(AGO2, AtAGO2, AT1G31280) (ARGONAUTE 2) is likely to be involved in RNA binding and/or cleavage Arabidopsis thaliana
(AGO9, AT5G21150) (ARGONAUTE 9) is likely to be involved in RNA binding and/or cleavage Arabidopsis thaliana
resulting small RNA is incorporated in RNA silencing complex (RISC)
vsiRNAs are loaded onto RNA-induced silencing complex (RISC)
RNA polymerase V functions independently of siRNA-directed DNA methylation pathway
base pairing between the plant gene fragment present in BMV and the plant target mRNA is suggested to result in a double-stranded structure targeted for removal from the virus sequence by enzymes in the RNAi pathway
resemblance of transgene arrangement with viral or bacterial genes has been proposed to be one of the main factors leading to siRNA production
terminators leading to transcripts with poorly defined poly(A) sites would be additional factor resulting in generation of siRNAs from transgenes
amiRNAs targeting both mature miRNA and stem-loop sequence direct RNA silencing through cleavage of miRNA precursor transcript Arabidopsis thaliana
mRNAs lacking poly(A) tails has been shown to attract (AtRDR6, RDR6, SDE1, SGS2, AT3G49500)
cleavage of precursor transcripts within the stem-loop sequence prevents dsRNA formation Arabidopsis thaliana
siRNAs matching promoters were 21–24 nt long size Nicotiana benthamiana
transcripts undergoing splicing show less RDR6-mediated siRNA production
Arabidopsis thaliana (RBCS1A, AT1G67090) terminator deficiency in Nicotiana benthamiana leads to siRNA accumulation Nicotiana benthamiana
viruses counter plant RNA silencing defense mechanism
transcription termination possibly, but not necessarily, protects genes against silencing by invoking transitivity Arabidopsis thaliana
siRNAs matching GFP could be readily detected in all samples where the reporter was expressed having tNOS or tRBCS as the 3′ regulatory sequence Nicotiana benthamiana
absence of (ATDCL4, DCL4, AT5G20320) results in (ATDCL2, DCL2, AT3G03300) becomes the major enzyme processing dsRNAs
22 nt long siRNA molecules is hallmark of (ATDCL2, DCL2, AT3G03300) Arabidopsis thaliana
siRNAs originating from the 3′end of the promoters analyzed as an indication of a putative susceptibility of the different 5′ regulatory region to methylation Nicotiana benthamiana
all constructs with HSP terminator and low levels of siRNA targeting the transgene siRNA levels Nicotiana benthamiana
lines S10, S20, S39, S40 and S41 could detect increase in accumulation of siRNAs originating from the 'S' region of (ATCHS, CHS, TT4, AT5G13930) and from GFP Arabidopsis thaliana
anti-159 amiRNAs directed RNA silencing via cleavage of miRNA primary transcripts Arabidopsis thaliana
introns in combination with certain terminators such as tNOS could act as catalyst for siRNA production
anti-164abc sRNA was designed to express 21-nt silencing signal perfectly complementary to (MIR164, MIR164A, AT2G47585) and (MIR164, MIR164B, AT5G01747) Arabidopsis thaliana
differences in the efficiency and accuracy of transcription termination could explain the spectrum of terminator-dependent siRNA accumulation Nicotiana benthamiana
plants produce small RNAs
RNA silencing diversity expands understanding of tissue-specific activities of RNA silencing in eukaryotic organisms
miRNA-directed silencing of mRNAs in plants is generally believed to occur in cytoplasm Plantae
amiRNAs designed to silence the (EEP1, MIR164, MIR164C, AT5G27807) family designed to target stem-loop region of PRI-MIR164B Arabidopsis thaliana
amiRNA-directed cleavage of a pri-miRNA transcript downstream of the pre-miRNA stem-loop region has no effect on pre-miRNA stem-loop formation Arabidopsis thaliana
viral suppressors of RNA silencing (VSRs) target different pathways and/or components of the silencing machinery
anti-159a amiRNA directed effective RNA silencing against all three (MIR159, MIR159A, AT1G73687) family members Arabidopsis thaliana
AK-6B and AB-6B target RNA-silencing factors (AGO1, AtAGO1, ICU9, AT1G48410) and SERRATE
stalled spliceosomes trigger RNA interference yeast; Drosophila
amiRNA-loaded RISC directs cleavage of nuclear-localized pri-miRNA transcripts Arabidopsis thaliana
host-encoded siRNAs produced by OsNRPD1a/b may act as antiviral triggers via the RNA-silencing pathway Oryza sativa
terminators directly impact amount of siRNAs generated from a locus
amiRNA-loaded RISC functions in nucleus Arabidopsis thaliana
variation in siRNA accumulation targeting the transgene investigated whether this variation was the result of a different pattern in siRNA accumulation Nicotiana benthamiana
amiRNAs targeting PRI-MIR164B sequences outside of the stem-loop region (anti-164bus and anti-164bds) failed to reduce (EEP1, MIR164, MIR164C, AT5G27807) accumulation Arabidopsis thaliana
dsRNAs are recognized and processed by dsRNA-specific RNAses, called Dicer-like (DCL) proteins
sRNA is loaded by large AGO protein containing complexes Mammalia
improperly terminated, unpolyadenylated transcripts strongly recruit (AtRDR6, RDR6, SDE1, SGS2, AT3G49500)
small interfering RNAs from the host are transported into filamentous organism
small interfering RNAs from filamentous organism are transported into host plant cells
small RNA-programmed ARGONAUTES (AGOs) target and silence complementary DNA through transcriptional gene silencing (TGS)
virus-activated siRNAs (vasiRNAs) function mainly through (AGO2, AtAGO2, AT1G31280) Arabidopsis thaliana
RNA silencing apparatus processes long npcRNAs Arabidopsis thaliana
(AGO1, AtAGO1, ICU9, AT1G48410) is catalytic core of miRNA-loaded RISC Arabidopsis thaliana
Slicer endonuclease activity of (AGO1, AtAGO1, ICU9, AT1G48410) has been shown to specifically co-elute with small protein complexes Arabidopsis thaliana
sRNA loaded by large AGO protein containing complexes in the cytoplasm can subsequently be imported back into nucleus Mammalia
DCL2-dependent 22nt siRNAs trigger transitivity from GFP-S reporter
ethylene-inducible transcription factor (AtRAV2, EDF2, RAP2.8, RAV2, TEM2, AT1G68840) is required for inhibition of RNA silencing by turnip crinkle virus (TCV) silencing suppressor
amiRNAs designed to silence the (EEP1, MIR164, MIR164C, AT5G27807) family designed to target upstream region of PRI-MIR164B Arabidopsis thaliana
anti-164bsl amiRNA was designed to target loop region of the stem-loop sequence of PRE-MIR164B Arabidopsis thaliana
siRNA generation leads to gene silencing
viruses can exploit endogenous RNA silencing pathways
VIGS originates in small RNA-based antiviral response
silencing RNA traffic between filamentous organisms and plant hosts
(CHR38, CLSY, CLSY1, AT3G42670) proteins is versatile component of RNA silencing
anti-164bus and anti-164bds amiRNAs were effectively guiding cleavage of targeted transcript Arabidopsis thaliana
production of secondary siRNAs indirectly improves detection of silencing of the GFP-S reporter Arabidopsis thaliana
sRNA population from introns near inefficient terminator would not only promote gene silencing
RNA silencing participates in RNA-directed DNA methylation
amiRNAs designed to silence the (EEP1, MIR164, MIR164C, AT5G27807) family designed to target mature (EEP1, MIR164, MIR164C, AT5G27807) sequence Arabidopsis thaliana
nuclear silencing of nuclear transcripts occurs via mRNA degradation Mammalia
anti-164abc sRNA directed RNA silencing against all (EEP1, MIR164, MIR164C, AT5G27807) family members Arabidopsis thaliana
(AtRDR6, RDR6, SDE1, SGS2, AT3G49500) attraction to mRNAs lacking poly(A) tails resulting in siRNA production
strong terminator decreases siRNA production
small RNA-programmed ARGONAUTES (AGOs) target and silence complementary RNA through posttranscriptional gene silencing (PTGS)
(ARID1, AT2G46040) is interactor of RNA silencing factors
non-canonical sRNAs derived from functional non-coding RNAs is underexplored aspect of RNA silencing
RNA silencing involves biogenesis of microRNAs (miRNAs)
AGOs associate with endogenous small RNAs
host plant interactions of filamentous organisms are influenced by RNA silencing networks
vsiRNA (vsiR45349) is complementary to NbFD1 open reading frame Nicotiana benthamiana
R-vsiR45349 overexpression reduces endogenous NbFD1 transcript levels Nicotiana benthamiana
virus-activated siRNAs (vasiRNAs) are (ATDCL4, DCL4, AT5G20320) /RDR1-dependent Arabidopsis thaliana
anti-159 sRNAs directed silencing against (MIR319, MIR319B, AT5G41663) family Arabidopsis thaliana
(ATDCL2, DCL2, AT3G03300) is known to process dsRNAs in the absence of (ATDCL4, DCL4, AT5G20320) Arabidopsis thaliana
intron retention was triggered by siRNAs Nicotiana benthamiana
RNA helicase requirement for sRNA-dependent gene silencing is only necessary for intron-containing genes Caenorhabditis elegans
Argonautes (AGOs) associate with small RNAs
RNA silencing has detrimental consequences
R-vsiR45349 overexpression reduces accumulation of NbFD1-GFP protein Nicotiana benthamiana
virus disease is used as template for interpretation of RNA silencing in connection with filamentous organisms and infected plant cells
PSR1 inhibits RNA silencing Glycine max
downregulation of SAHH activity leads to suppression of local silencing
four members of the DICER family coupled to 10 ARGONAUTE (AGO) members Arabidopsis thaliana
silencing mechanisms lead to generation of heterochromatin-siRNAs
second series of amiRNAs was designed to target three structurally distinct regions of the (MIR164, MIR164B, AT5G01747) precursor transcript Arabidopsis thaliana
P19 binding to 21 nt sRNAs blocking PTGS
investigation of RNA silencing resulted in elucidation of gene regulatory mechanisms occurring in most eukaryotes
vsiRNA-mediated host mRNA cleavage may not be widespread Vitis vinifera
(AGO4, OCP11, AT2G27040) associates with endogenous small RNAs Arabidopsis thaliana
CMV infection induces synthesis of 21 nt siRNAs Arabidopsis thaliana
poly(A) tail blocks (AtRDR6, RDR6, SDE1, SGS2, AT3G49500) activity
stabilized siRNAs are loaded onto RNA-induced transcriptional silencing (RITS) complex or RNA-induced silencing complex (RISC)
Brassica napus ZWILLE-1 (BnZLL-1) is orthologous to Arabidopsis thaliana ZWILLE (AtZLL) Brassica napus; Arabidopsis thaliana
DICER-LIKE 1 (ASU1, ATDCL1, CAF, DCL1, EMB60, EMB76, SIN1, SUS1, AT1G01040) DICER-LIKE 3 (ATDCL3, DCL3, AT3G43920) and DICER-LIKE 4 (ATDCL4, DCL4, AT5G20320) act as the primary processors of three respective classes of endogenous silencing RNAs Arabidopsis thaliana
hypomorphic (AtRDR6, RDR6, SDE1, SGS2, AT3G49500) mutants are impaired in sense-transgene-induced posttranscriptional gene silencing Arabidopsis thaliana
Cucumber mosaic virus 2b protein inhibits AGO1's cleavage activity in RISC
RNA silencing involves biogenesis of small interfering RNAs (siRNAs)
downregulation of SAHH activity leads to reduced RDR6-dependent accumulation of secondary siRNAs
microRNA (miRNA) targets for cleavage mRNA
silencing components are strongly downregulated in cv. Rywal at 1 and 3 dpi Solanum tuberosum
Small interference RNAs (siRNAs) are generated from double-stranded RNAs
21-nt sRNA molecules mediate cytoplasmic RNA cleavage
HvDRF1 construct caused degradation of target:GFP fusion RNA Hordeum vulgare
cis-NATs generate nat-siRNA sequences Arabidopsis thaliana; Oryza sativa
endogenous 24-nucleotide siRNAs are often associated with ARGONAUTE 4 (AGO4, OCP11, AT2G27040) and ARGONAUTE 6 (AGO6, AT2G32940)
(AtRDR6, RDR6, SDE1, SGS2, AT3G49500) is required for induction of de novo silencing
TAS gene transcripts are cleaved by miRNAs
TGS-inducing wild-type Arabidopsis grafted onto wild-type reporter line transmits 21–24 nucleotide small RNAs (21–24 nt sRNAs) Arabidopsis thaliana
DICER-LIKE 1 (ASU1, ATDCL1, CAF, DCL1, EMB60, EMB76, SIN1, SUS1, AT1G01040) is required for the accumulation of 21- and 24-nucleotide siRNAs processed from IR transgenes
differences in phenotypic effects of three Dhn-RNAi constructs was due to different RNAi efficiency Hordeum vulgare
miRNAs induce trans-acting small interfering RNA (ta-siRNA)
AGO proteins silence target genes by RNA cleavage, RNA degradation, or translation inhibition Arabidopsis thaliana
phasiRNAs demonstrated to be in phase with predicted amiRNA-directed cleavage site Arabidopsis thaliana
heterogeneous sRNA population from 22-nt amiRNA-directed phasiRNA production allows multiple target complementarity not possible with conventional amiRNA vectors
Beet western yellows virus P0 can lead to decay of ARGONAUTEs
RNA-DEPENDENT RNA POLYMERASE6 (AtRDR6, RDR6, SDE1, SGS2, AT3G49500) -mediated accumulation of phasiRNAs mediates widespread RNA silencing of endogenous CHALCONE SYNTHASE (ATCHS, CHS, TT4, AT5G13930) target gene Arabidopsis thaliana
hairpin RNA (hpRNA) transgenes are used in knock-down of invading viruses
SSU_hpCHS-mediated silencing is observed in all tissues of mature leaves, including the vasculature
transgenic sweet potato over-expressing pre-sRNA8105 gene shows induction of IbMYB1-siRNA without wounding Ipomoea batatas
Tomato bushy stunt virus P19 binds siRNA duplexes
P0-triggered degradation of (AGO1, AtAGO1, ICU9, AT1G48410) suppresses gene silencing Arabidopsis thaliana
trans-acting siRNAs (tasiRNAs) are generated from single-stranded RNAs
(ATTAS3, sORF24, TAS3, TASIR-ARF, AT3G17185) matching (MIR166, MIR166G, AT5G63715) precursor genes may account in part for (MIR166, MIR166G, AT5G63715) upregulation in (AS2, AT1G65620) (AtRDR6, RDR6, SDE1, SGS2, AT3G49500) Arabidopsis thaliana
null (AtRDR6, RDR6, SDE1, SGS2, AT3G49500) mutants lack TAS1 ta-siRNAs Arabidopsis thaliana
null (ATSGS3, SGS3, AT5G23570) mutants lack (ATTAS3, sORF24, TAS3, TASIR-ARF, AT3G17185) ta-siRNAs Arabidopsis thaliana
(ATTIF3K1, DRB4, AT3G62800) mutants have TAS1 ta-siRNAs in leaves Arabidopsis thaliana
(ATTIF3K1, DRB4, AT3G62800) interacts with (ATDCL4, DCL4, AT5G20320) Arabidopsis thaliana
genes involved in Alzheimer's disease can generate nat-siRNAs Mus musculus
P0 protein may have other cellular targets
Dicer-like (DCL) proteins generate 21, 22, and 24 nucleotide small RNAs (sRNAs) Arabidopsis thaliana
sequence divergence between Arabidopsis thaliana and Arabidopsis arenosa ATHILA elements might reduce specificity of maternally (At) contributed siRNA Arabidopsis thaliana; Arabidopsis arenosa
(ATTAS3, sORF24, TAS3, TASIR-ARF, AT3G17185) also matches one of six precursor genes for the abaxial microRNA (MIR166, MIR166G, AT5G63715) Arabidopsis thaliana
miRNAs loaded onto AGO proteins Arabidopsis thaliana
14 (73.7%) of the 19 precursors can be associated with 5 AGO proteins Arabidopsis thaliana
nat-siRNA sequences are enriched in overlapping regions of cis-NAT pairs Arabidopsis thaliana; Oryza sativa
siRNA clusters that appear exclusively in overlapping regions of NATs support that at least some siRNAs are caused by cis-NAT configuration Arabidopsis thaliana; Oryza sativa
small RNA molecules of 21 nt (siRNAs or miRNAs) guide the cleavage of target transcripts that contain sequences complementary to them
(AtRDR6, RDR6, SDE1, SGS2, AT3G49500) is required for systemic silencing Arabidopsis thaliana
2b protein from CMV exhibited high affinity for 21 and 24 nt siRNAs Arabidopsis thaliana
hpRNA-derived (ATCHS, CHS, TT4, AT5G13930) 'H' region-specific siRNAs detectable only in (ATMYB75, AtPAP1, MYB75, PAP1, PAP1-D, SIAA1, AT1G56650) transformants Arabidopsis thaliana
RNA silencing via sense/antisense inverted-repeat RNAs is used for silencing of Chlamydomonas nuclear genes Chlamydomonas reinhardtii
knockdown approaches have limitation of incomplete suppression of target gene function Chlamydomonas reinhardtii
small RNAs (sRNAs) act as mobile signals for regulation of gene expression
IbMYB1-siRNA induction results in decreased expression of IbMYB1 gene family Ipomoea batatas
(ATCPSF100, CPSF100, EMB1265, ESP5, AT5G23880) plays a role in RNA silencing Arabidopsis thaliana
small RNA silencing complex RISC mediates miRNA or siRNA functions Arabidopsis thaliana
RISC cleaves via AGO1 slicer activity complementary target mRNAs
DICER-like (DCL) endoribonucleases 2, 3 and 4 are predominant DCL endoribonucleases involved in silencing of positive-sense RNA viruses Arabidopsis thaliana
(AGO1, AtAGO1, ICU9, AT1G48410) is major player in virus-induced silencing in all tissue types Arabidopsis thaliana
relationships between miRNA-like RNAs and targets reminiscent of miRNAs and their targets Arabidopsis thaliana
(AtRDR6, RDR6, SDE1, SGS2, AT3G49500) (RNA-DEPENDENT RNA POLYMERASE 6) is required for generation of trans-acting small interfering RNA (ta-siRNA) Arabidopsis thaliana
absence of a functional THO/TREX complex or (AGO1, AtAGO1, ICU9, AT1G48410) (ATSGS3, SGS3, AT5G23570) and (AtRDR6, RDR6, SDE1, SGS2, AT3G49500) results in RNA silencing operating less efficiently Arabidopsis thaliana
tools delineated in this study can be used to study complexity of RNA silencing pathways in plants Arabidopsis thaliana
miRNA-like RNAs can potentially function through AGO proteins Arabidopsis thaliana
dcl3-5 mutation causes impaired production of 24 nt sRNA
dsRNAs formed within overlapping regions of sense and antisense transcripts are sufficient for producing nat-siRNAs Arabidopsis thaliana; Oryza sativa
plant sRNAs derived from plant cis-NATs are enriched in their overlap regions Arabidopsis thaliana; Oryza sativa
(AtRDR6, RDR6, SDE1, SGS2, AT3G49500) is required for perception of the signal
(AGO7, ZIP, AT1G69440) mutants lack (ATTAS3, sORF24, TAS3, TASIR-ARF, AT3G17185) ta-siRNAs in leaves Arabidopsis thaliana
(AGO7, ZIP, AT1G69440) mutants have (TAS2, AT2G39681) ta-siRNAs in leaves Arabidopsis thaliana
12 miRNA-like RNAs can be associated with (AGO4, OCP11, AT2G27040) Arabidopsis thaliana
ablation of three miRNA-like RNAs (miR169i.2-3p, miR169j.2, and miR839.3) in dcl1-9 mutant led to elevated expression of some targets Arabidopsis thaliana
siRNAs from cis-NATs are dependent on DICER-LIKE 1 (ASU1, ATDCL1, CAF, DCL1, EMB60, EMB76, SIN1, SUS1, AT1G01040) and/or DICER-LIKE 3 (ATDCL3, DCL3, AT3G43920) Arabidopsis thaliana; Oryza sativa
silencing of CHS-A genes in P. hybrida may result from a different mechanism than silencing induced by transcribed inverted repeats Petunia hybrida
mechanistic details of RNA silencing may vary in genetically closely related plant species
phasiRNA production is derived from complementary targets Arabidopsis thaliana
hairpin RNA (hpRNA) transgenes are used in knock-down of homologous transgenes
hpRNA transgene expression shows instability in hpRNA-mediated RNA silencing
mobile silencing signal moves between cells and through phloem Arabidopsis thaliana
persistent epigenetic changes may influence growth, development, and heritable phenotypes Arabidopsis thaliana
21-nucleotide siRNAs are generated by DICER-LIKE 4 (ATDCL4, DCL4, AT5G20320)
LRRTM1/Ctnna2 cis-NAT gene pair can generate nat-siRNAs Mus musculus
siRNA-induced gene silencing in Arabidopsis spreads from cell to cell in relay mechanism Arabidopsis thaliana
P0 effect on accumulation of miRNA-target genes paralleling (AGO1, AtAGO1, ICU9, AT1G48410) mutant effect further supports hypothesis that (AGO1, AtAGO1, ICU9, AT1G48410) is a target of P0 Arabidopsis thaliana
six miRNA-like RNAs (miR159a.2-3p, miR169b.2, miR169i.2-3p, miR169j.2, miR822.4-5p, miR839.3) have targets supported by degradome data Arabidopsis thaliana
ZWILLE/ (AGO10, PNH, ZLL, AT5G43810) negatively regulates (AGO1, AtAGO1, ICU9, AT1G48410) protein levels Arabidopsis thaliana
22-nt amiRNA expression from an asymmetric duplex triggers CHALCONE SYNTHASE (ATCHS, CHS, TT4, AT5G13930) silencing in all tissues including roots and seed coats Arabidopsis thaliana
secondary siRNAs repress expression of IbMYB1 gene family Ipomoea batatas
processing of microRNA precursor molecules involves Dicer-like enzymes Arabidopsis thaliana
this study provides several new insights into ability of (ATDCL4, DCL4, AT5G20320) to process distinct dsRNA substrates Arabidopsis thaliana
5′ terminal U to A substitution of amiR-trichome prevents miRNA-mediated reduction of target gene expression Arabidopsis thaliana
virus vectors are used in knock-down of homologous transgenes
(ATMYB75, AtPAP1, MYB75, PAP1, PAP1-D, SIAA1, AT1G56650) /SSU_amiR21-CHS and /SSU_amiR22-CHS lines most abundant sRNA reads corresponded to predicted amiRNA guide strands Arabidopsis thaliana
15 miRNA-like RNAs can be associated with (AGO2, AtAGO2, AT1G31280) Arabidopsis thaliana
SUPPRESSOR OF GENE SILENCING 3 (ATSGS3, SGS3, AT5G23570) is assumed to stabilize template RNA fragments before double-stranded (ds)RNA synthesis is performed by (AtRDR6, RDR6, SDE1, SGS2, AT3G49500) Arabidopsis thaliana
relevant target of RNA silencing in the context of scopolin accumulation might be repressor of scopolin synthesis Arabidopsis thaliana
small RNAs (sRNAs) can act in homology-dependent manner to guide transcriptional and post-transcriptional silencing
silencing complexes pair to target transcripts
(AGO1, AtAGO1, ICU9, AT1G48410) was purified (AGO1, AtAGO1, ICU9, AT1G48410) complex Arabidopsis thaliana
silencer (S) transgene specifies hairpin RNA
other 24 nt sRNA loci produce nonmobile species
large population of CHS-A-related sRNA molecules in white floral tissues consists of siRNA molecules processed from double-stranded RNA from exon 2 regions Petunia hybrida
plant miRNAs function like siRNAs to guide target RNA cleavage
amiR-trichome is not incorporated into (AGO2, AtAGO2, AT1G31280) complex Arabidopsis thaliana
(ATTAS3, sORF24, TAS3, TASIR-ARF, AT3G17185) ta-siRNAs are targets of (ARF4, AT5G60450) mRNA Arabidopsis thaliana
miRNA-like RNAs can be associated with AGO proteins Arabidopsis thaliana
miR822.4-5p in dcl4-2 mutant led to elevated expression of some targets Arabidopsis thaliana
cis-NATs are processed by DICER-LIKE 1 (ASU1, ATDCL1, CAF, DCL1, EMB60, EMB76, SIN1, SUS1, AT1G01040) and/or DICER-LIKE 3 (ATDCL3, DCL3, AT3G43920) Arabidopsis thaliana; Oryza sativa
(HESO1, AT2G39740) may have other substrates in vivo 5' fragments of RNA-induced silencing complex cleavage products Arabidopsis thaliana
DICER-LIKE 1 (ASU1, ATDCL1, CAF, DCL1, EMB60, EMB76, SIN1, SUS1, AT1G01040) and/or DICER-LIKE 3 (ATDCL3, DCL3, AT3G43920) generate 20- to 22-nucleotide short siRNAs and 23- to 28-nucleotide long siRNAs Arabidopsis thaliana; Oryza sativa
sRNA molecules with sequences related to PhCHS-A1 and PhCHS-A2 were generated predominantly in white tissues of Picotee petunia Petunia hybrida
different pathways in which si/miRNAs can act are tightly linked to presence of specific RNA-binding proteins
anti-164abc amiRNA was also predicted to be capable of targeting (EEP1, MIR164, MIR164C, AT5G27807) Arabidopsis thaliana
(EEP1, MIR164, MIR164C, AT5G27807) accumulation only appeared reduced in anti-164abc and anti-164bsl plants Arabidopsis thaliana
(ATTAS3, sORF24, TAS3, TASIR-ARF, AT3G17185) ta-siRNAs are targets of (ARF3, ETT, AT2G33860) /ETTIN mRNA Arabidopsis thaliana
(ATTIF3K1, DRB4, AT3G62800) mutants lack (ATTAS3, sORF24, TAS3, TASIR-ARF, AT3G17185) ta-siRNAs in leaves Arabidopsis thaliana
anti-159c amiRNA directed effective RNA silencing against all three (MIR159, MIR159A, AT1G73687) family members Arabidopsis thaliana
(AtRDR6, RDR6, SDE1, SGS2, AT3G49500) would be more available to intronless transcripts
VSR p38 from TCV interferes with loading of double-stranded RNA into (AGO1, AtAGO1, ICU9, AT1G48410) /2-RISC complex plant systems
changed abundance of tasiRNA precursors and microRNAs in (AtHPR1, AtTHO1, HPR1, RAE3, THO1, AT5G09860) mutants support contribution of THO/TREX complex to tasiRNA formation and accumulation of the respective precursors Arabidopsis thaliana
(AGO5, AtAGO5, AT2G27880) bound 21, 22, and 24 nucleotide small RNAs Arabidopsis thaliana
Argonaute (AGO) facilitates endonucleolytic cleavage of transcripts
RNA induced silencing complexes (RISCs) contain ARGONAUTE (AGO) or PIWI family proteins
vsiRNA1 could specifically cleave TaAAED1 mRNA
bifunctional nuclease-2 (BN2) indirectly derepresses silencing of (AGO1, AtAGO1, ICU9, AT1G48410) /2
knockdown approaches have limitation of loss of under-expression phenotype by silencing of suppression construct Chlamydomonas reinhardtii
nat-siRNAs that require RDRs are not completely eliminated in rdr mutants Arabidopsis thaliana; Oryza sativa
nat-siRNAs may be amplified by RDRs in a secondary amplification step Arabidopsis thaliana; Oryza sativa
small non-coding RNAs (sRNAs) control through post-transcriptional mechanisms defense against transposable elements and viruses
different pathways in which si/miRNAs can act have some steps occurring in nucleus
amiRNA-directed silencing of an endogenous miRNA is due to cleavage of precursor transcripts within the stem-loop sequence Arabidopsis thaliana
miR393b* is AGO2-bound small RNA Arabidopsis thaliana
hc-siRNA duplexes are loaded into (AGO4, OCP11, AT2G27040)
ARR is linked to RNA silencing during viral infection
viral silencing suppressor P19 expression in root stele antagonizes (MIR399, MIR399F, AT2G34208) silencing effect Arabidopsis thaliana
sRNAs are loaded into Argonaute proteins (AGOs)
GFP siRNAs are not detected in (AGO4, OCP11, AT2G27040) immunoprecipitates Arabidopsis thaliana
(AGO1, AtAGO1, ICU9, AT1G48410) mutant background allows detection of SDE3-AGO2 interactions Arabidopsis thaliana
(CAT2, AT4G35090) dsRNA was produced by (AtRDR2, RDR2, SMD1, AT4G11130) or (AtRDR6, RDR6, SDE1, SGS2, AT3G49500) Arabidopsis thaliana
degree of involvement of (AtRDR2, RDR2, SMD1, AT4G11130) and (AtRDR6, RDR6, SDE1, SGS2, AT3G49500) in the production of secondary siRNAs and the spread of methylation depends on silencing configuration
artificial TAS (aTAS) transcripts offer greater mobility than amiRNAs or hpRNA-derived siRNAs
phasiRNA production scales with target transcript abundance Arabidopsis thaliana
35S promoter-driven hpRNA expressing lines do not show detection of transitively produced siRNAs
sRNA8105 and IbMYB1 interaction is confirmed by cleavage site mapping; agro-infiltration analyses; transgenic sweet potato over-expressing pre-sRNA8105 Ipomoea batatas
amiRNAs targeting the mature (EEP1, MIR164, MIR164C, AT5G27807) (anti-164abc) effectively reduced accumulation of (EEP1, MIR164, MIR164C, AT5G27807) Arabidopsis thaliana
eukaryotes possess effective RNA silencing machinery
miRNAs can direct RNA cleavage and RNA silencing Arabidopsis thaliana
P19 is known to bind 21 nt long sRNAs
DCL2-dependent siRNAs appear to trigger transitivity from their targets
GFP under the control of the p35S led to low siRNA production when used in combination with tHSP
RNA helicase is required for sRNA-dependent gene silencing Caenorhabditis elegans
combination of an intron in the proximity of the NOS terminator leads to production of siRNAs
transitively produced 'S' region-specific siRNAs detected at low level in (ATMYB75, AtPAP1, MYB75, PAP1, PAP1-D, SIAA1, AT1G56650) transformants Arabidopsis thaliana
processing or effector components of siRNA pathway may be limited in young leaves and petioles Arabidopsis thaliana
saturation of components of the silencing machinery, such as (AGO1, AtAGO1, ICU9, AT1G48410) might cause interference with developmentally important regulation
microRNAs appear to be cell autonomous Arabidopsis thaliana
DCL1-dependent 21-nucleotide nat-siRNA is generated from same site in overlapping region of NAT pair Arabidopsis thaliana
double-stranded RNAs can potentially be processed by DICER-LIKE 1 (ASU1, ATDCL1, CAF, DCL1, EMB60, EMB76, SIN1, SUS1, AT1G01040) and/or DICER-LIKE 3 (ATDCL3, DCL3, AT3G43920) Arabidopsis thaliana; Oryza sativa
mRNA stability and/or translatability are affected by action of micro RNAs (miRNAs)
22-nt amiRNA that triggers phasiRNA production overcomes limited distribution of (AtSSU, GGPPS12, GGR, SSU, AT4G38460) promoter-driven amiRNA and hpRNA-mediated (ATCHS, CHS, TT4, AT5G13930) silencing
21- and 22-nucleotide siRNAs are generated by DICER-LIKE 2 (ATDCL2, DCL2, AT3G03300)
relatively weak (AtSSU, GGPPS12, GGR, SSU, AT4G38460) promoter-driven hpCHS facilitates detection of transitive siRNAs
Arabidopsis thaliana THO/TREX complex contains (AtRDR6, RDR6, SDE1, SGS2, AT3G49500) Arabidopsis thaliana
inactivity of RNA silencing pathway allows expression of the negative regulator of scopolin synthesis Arabidopsis thaliana
siRNAs are incorporated into silencing complexes
legumes can synthesize siRNAs triggered in a phased register by 22-nt miRNAs on specific mRNAs (phasiRNAs)
compartmentalization of siRNA pools is important for individual functions of siRNA pools
virus vectors are used in knock-down of invading viruses
limited transitive amplification of silencing signal from over-expressed endogenous (ATCHS, CHS, TT4, AT5G13930) transcript may account for detectable quantity of both 'H' and 'S' region-specific siRNAs Arabidopsis thaliana
(AtRDR6, RDR6, SDE1, SGS2, AT3G49500) mutants display similar silencing distribution to 21-nt amiRNA lines
enhanced silencing in SSU_amiR22-CHS lines may be attributed to greater mobility of phasiRNAs
production of phasiRNAs is in proportion to target transcript abundance
asymmetric 22-nt amiRNA-directed RNA silencing is expected to be effective in suppressing other genes and/or members of gene families Arabidopsis thaliana
artificial TAS (aTAS) transcripts mediate effective RNA silencing of complementary targets
SDE3-FHA is detected in (AGO2, AtAGO2, AT1G31280) immunoprecipitates from (AGO1, AtAGO1, ICU9, AT1G48410) mutants Arabidopsis thaliana
GFP-AGO1 binds miRNA Arabidopsis thaliana
host Dicer-Like (DCL) enzymes generate virus-derived small interference RNAs (vsiRNAs)
RNA silencing pathways that involve miRNAs as triggers and tasiRNAs as secondary signals have targets including Squamosa-promoter Binding Protein
mostly 21 nucleotide smRNAs emanate from entire length of endogenous functionally annotated transcripts Arabidopsis thaliana
hpRNA expression does not trigger CHALCONE SYNTHASE (ATCHS, CHS, TT4, AT5G13930) silencing in seed coats Arabidopsis thaliana
phasiRNA dependent nature of widespread silencing phenotype validated by lack of detectable phasiRNA production in all plants harbouring (AtRDR6, RDR6, SDE1, SGS2, AT3G49500) mutation Arabidopsis thaliana
secondary siRNAs produced from (CAT2, AT4G35090) non-target sequences lack of secondary siRNA production Arabidopsis thaliana
22-nt amiRNA-directed silencing technology should facilitate application in diverse species
artificial miRNA (amiRNA) directs RNA silencing of selected targets
(POL, AT2G46920) II transcripts serve as templates for RNA-dependent RNA polymerase Schizosaccharomyces pombe
different miRNA-like RNAs from same miRNA precursor may have targets in same gene family Arabidopsis thaliana
SSU_hpCHS vector expressing lines do not show silencing of CHS in petioles and young leaves
limited or absent (AtSSU, GGPPS12, GGR, SSU, AT4G38460) promoter expression explains persistent anthocyanin accumulation in roots and seed coats
(ATUPF1, LBA1, UPF1, AT5G47010) is also present in silencing bodies
lack of transitive amplification of the silencing signal causes instability of hpRNA-mediated RNA silencing
amiRNA-directed silencing is more efficient than hpRNA-derived siRNA silencing
limited distribution of (AtSSU, GGPPS12, GGR, SSU, AT4G38460) promoter-driven 21-nt amiRNA- and hpRNA-mediated RNA silencing of an endogenous target gene, (ATCHS, CHS, TT4, AT5G13930) was overcome by use of a 22-nt amiRNA vector
SKP1–P0–AGO1 interaction complex disrupt host RNA silencing
(ATDCL4, DCL4, AT5G20320) mutant shows sRNAs associated with the 'S' and 'H' regions of (ATCHS, CHS, TT4, AT5G13930) and the GFP gene being generated as 22-nucleotide long molecules
VmR2 -siR1 cleaves MdLRP14 transcripts Malus domestica
(AtTEX1, RAE2, TEX1, THO3, AT5G56130) loss of has the strongest effect on tasiRNA accumulation Arabidopsis thaliana
trans-acting siRNAs (tasiRNAs) differ from classical siRNAs
double-stranded RNAs trigger silencing mechanisms
21-nt artificial microRNA (amiRNA) is compared with 22-nt artificial microRNA (amiRNA)-mediated RNA silencing Arabidopsis thaliana
asymmetric 22-nt amiRNA-directed RNA silencing is associated with phasiRNA production and activity Arabidopsis thaliana
hpRNA-derived (ATCHS, CHS, TT4, AT5G13930) 'H' region-specific siRNAs thought to remain below detection sensitivities in Col-0 transformants Arabidopsis thaliana
(AtRDR6, RDR6, SDE1, SGS2, AT3G49500) activity required for phasiRNA accumulation Arabidopsis thaliana
22-nt amiRNA functions effectively in local silencing
22-nt amiRNA-directed phasiRNA production offers advantages of heterogeneous sRNA population allowing multiple target complementarity
transgenic sweet potato over-expressing pre-sRNA8105 gene shows induction of IbMYB1-siRNA Ipomoea batatas
investigation of RNA silencing resulted in explanation of pathogen-derived resistance
single-stranded siRNAs bind mRNAs for targeted degradation
siRNAs that accumulate in sperm cells (SCs) are likely generated from reactivated Athila TE transcripts from the vegetative nucleus (VN)
AGO10-interacting partners may inhibit slicing function of (AGO10, PNH, ZLL, AT5G43810) Arabidopsis thaliana
TuMV triggers vasiRNA accumulation
RNA silencing mechanism is suggested to mediate scopolin accumulation in response to stress Arabidopsis thaliana
tasiRNA silencing pathway is initiated by ARGONAUTE (AGO)-mediated cleavage of single-stranded transcripts from TAS loci under guidance of an miRNA Arabidopsis thaliana
(AGO4, OCP11, AT2G27040) selectively binds to remaining 22-24 nt siRNAs
control conditions result in absence of initiating primary small-RNA signal Arabidopsis thaliana
mRNA stability and/or translatability are affected by action of short interfering RNAs (siRNAs)
Dicer-like (DCL) protein family produce small RNAs (sRNAs)
(ATMYB75, AtPAP1, MYB75, PAP1, PAP1-D, SIAA1, AT1G56650) background shows detection of transitively produced siRNAs
(AGO2, AtAGO2, AT1G31280) was immunopurified (AGO2, AtAGO2, AT1G31280) complex Arabidopsis thaliana
functional DExD/H-box helicase domain is required for efficient production of all DCL4-dependent sRNAs Arabidopsis thaliana
PSR2 impairs secondary small interfering RNA production
heterochromatic siRNAs are associated with DNA methylation
plant viruses reprogram host gene expression
(AtTHO6, DWA1, THO6, AT2G19430) loss of has effect on tasiRNA accumulation Arabidopsis thaliana
transposable elements (TEs) show selective loss and gain of siRNAs
putative SDE3-AGO2 interactions may be normally masked by dominance of AGO1-SDE3 interactions Arabidopsis thaliana
RNA silencing suppression screen led to identification of PSR1
antiviral RNA silencing mechanism regulates Nib gene expression
pathogen-derived RNA silencing suppressors (RSSs) relieve microRNA miR482-mediated suppression of resistance genes Solanum lycopersicum
study by Liu et al. (2021) explored RNA silencing–based WYMV pathosystem Triticum aestivum
Arabidopsis encodes Dicer-like (DCL) proteins Arabidopsis thaliana
S locus-specific sRNAs are specific to region of the inverted repeat in the S transgene
(ATDCL4, DCL4, AT5G20320) physically and functionally interacts in vivo with dsRNA-binding protein 4 (ATTIF3K1, DRB4, AT3G62800) Arabidopsis thaliana
small RNAs were extracted from purified AGO complexes Arabidopsis thaliana
(AtHPR1, AtTHO1, HPR1, RAE3, THO1, AT5G09860) (Ws) mutant showed increased (TAS1C, AT2G39675) precursor Arabidopsis thaliana
involvement of an RNA silencing mechanism might target negative regulator of scopolin synthesis Arabidopsis thaliana
RNA silencing being defective in tho and (AGO1, AtAGO1, ICU9, AT1G48410) (ATSGS3, SGS3, AT5G23570) and (AtRDR6, RDR6, SDE1, SGS2, AT3G49500) mutants results in repressor of scopolin synthesis being expressed at higher levels Arabidopsis thaliana
small interfering RNAs (siRNAs) regulate centromere function
dcl2-1 mutant affects distinct siRNA-dependent silencing pathway Arabidopsis thaliana
siRNA accumulation upon retrotransposition caused by stress has been described previously
Arabidopsis thaliana THO/TREX complex contains (AGO1, AtAGO1, ICU9, AT1G48410) Arabidopsis thaliana
(AGO4, OCP11, AT2G27040) RNA-IP (RIP) showed (AGO4, OCP11, AT2G27040) selectively binds to remaining 22-24 nt siRNAs but not longer P4RNAs
small RNA Northern blotting examined abundance of siRNAs corresponding to 180-bp centromere repeats Arabidopsis thaliana
(ATCHS, CHS, TT4, AT5G13930) over-expression in (ATMYB75, AtPAP1, MYB75, PAP1, PAP1-D, SIAA1, AT1G56650) background facilitates visual detection of local and systemic RNA silencing Arabidopsis thaliana
Dicer-like (DCL) protein family process from double-stranded RNA (dsRNA) precursor transcripts
SSU_amiR22-CHS vector triggered production of abundant phasiRNAs 3′ from miRNA target site Arabidopsis thaliana
anthocyanin accumulation in roots and seed coats persisted in (AtRDR6, RDR6, SDE1, SGS2, AT3G49500) background despite localised miRNA-directed RNA silencing Arabidopsis thaliana
(AtHPR1, AtTHO1, HPR1, RAE3, THO1, AT5G09860) (Ws) mutant showed precursor of miR164 present at increased levels Arabidopsis thaliana
(AtHPR1, AtTHO1, HPR1, RAE3, THO1, AT5G09860) loss of has effect on tasiRNA accumulation Arabidopsis thaliana
RNA-dependent RNA polymerase (AtRDR6, RDR6, SDE1, SGS2, AT3G49500) is responsible for conversion of TAS transcript cleavage products into a double-stranded form Arabidopsis thaliana
(AGO10, PNH, ZLL, AT5G43810) DDH mutants form unproductive RISC
ARGONAUTE 4 (AGO4, OCP11, AT2G27040) may function as catalytic engine of RNA cleavage Arabidopsis thaliana
stress results in presence of initiating primary small RNA Arabidopsis thaliana
(ATSGS3, SGS3, AT5G23570) protein is thought to stabilize resulting cleavage fragments Arabidopsis thaliana
siRNA biogenesis has implications for recognition and silencing of aberrant RNA Arabidopsis; moss
amiRNA guide strand directs highly efficient RNA silencing of exogenous or endogenous target transcripts
anti-164bsl amiRNA did not induce silencing against pri-miR164a Arabidopsis thaliana
transcription termination has a pivotal role with respect to protecting genes against silencing Arabidopsis thaliana
siRNAs matching promoters in most but not all cases, were detected in samples showing high levels of sRNAs against GFP Nicotiana benthamiana
intron retention in tNOS-containing constructs is supported by the fact that reporters using the tRBCS, the other terminator alongside the tNOS leading to high levels of siRNA accumulation, also displayed splicing defects Nicotiana benthamiana
miR164-targeting amiRNAs directed efficient cleavage of pri-miR164b Arabidopsis thaliana
increase in transcripts that could become template for (AtRDR6, RDR6, SDE1, SGS2, AT3G49500) positively affects production of secondary siRNAs Arabidopsis thaliana
(UBP1B, AT1G17370) is targeted by siRNA854
RNA cleavage and RNA silencing directed by miRNAs occurs in nucleus Arabidopsis thaliana
ktf1-1 mutant is proficient in siRNA accumulation Arabidopsis thaliana
(NERD, AT2G16485) is possibly implicated in RNA silencing Arabidopsis thaliana
plant miRNAs are taken up in RISC complex
24-nt siRNAs associate with (AGO4, OCP11, AT2G27040) and other proteins
amiRNAs designed to silence the (EEP1, MIR164, MIR164C, AT5G27807) family designed to target downstream region of PRI-MIR164B Arabidopsis thaliana
anti-164abc amiRNA mediated silencing of (EEP1, MIR164, MIR164C, AT5G27807) Arabidopsis thaliana
few GFP-derived siRNAs were detected in leaves inoculated with constructs containing the tHSP Nicotiana benthamiana
anti-159b amiRNA directed effective RNA silencing against all three (MIR159, MIR159A, AT1G73687) family members Arabidopsis thaliana
viruses express RNA-silencing suppressor proteins
ta-siRNAs are loaded into (AGO1, AtAGO1, ICU9, AT1G48410)
amiRNA-directed cleavage of a pri-miRNA transcript upstream of the pre-miRNA stem-loop region has no effect on pre-miRNA stem-loop formation Arabidopsis thaliana
endogenous miRNAs can be efficiently silenced using amiRNA technology Arabidopsis thaliana
(AtRDR6, RDR6, SDE1, SGS2, AT3G49500) recruitment by improperly terminated transcripts induces PTGS
blocking of (AtRDR6, RDR6, SDE1, SGS2, AT3G49500) activity by poly(A) tail prevents production of siRNAs from mRNAs
four members of the DICER family and 10 ARGONAUTE (AGO) members preclude enormous complexity of the different pathways in which si/miRNAs can act Arabidopsis thaliana
small RNA (sRNA)-guided RNA cleavage occurs in cytoplasm Arabidopsis thaliana
amiRNAs designed against the mature miRNA sequence should allow for silencing of all miRNA family members Arabidopsis thaliana
anti-164bus amiRNA was designed to target 5' upstream sequences of PRI-MIR164B Arabidopsis thaliana
anti-164bds amiRNA was designed to target 3' downstream sequences of PRI-MIR164B Arabidopsis thaliana
anti-164bsl amiRNA directed RNA silencing specifically against (MIR164, MIR164B, AT5G01747) Arabidopsis thaliana
RNA silencing plays a major role in virus resistance in plants
increased availability of intronless transcripts to (AtRDR6, RDR6, SDE1, SGS2, AT3G49500) increases amount of siRNA being produced
improperly terminated transcripts with multiple cleavage sites including unpolyadenylated ones leads to (AtRDR6, RDR6, SDE1, SGS2, AT3G49500) recruitment
viruses overcome resistance based on RNA silencing
S-adenosyl methionine synthetase virus-induced activity changes debilitate post-transcriptional RNA silencing
RNAi pathways have been largely diversified and amplified plants
siRNAs specific to the 'C' region of (ATCHS, CHS, TT4, AT5G13930) were not observed Arabidopsis thaliana
amiRNAs designed against the stem-loop region of a pre-miRNA transcript should allow for silencing of a specific family member Arabidopsis thaliana
long non-protein-coding transcripts can be targeted by miRNA-directed silencing Arabidopsis thaliana
(ATDCL4, DCL4, AT5G20320) has described role in RNA silencing pathway Arabidopsis thaliana
miRNAs that are 22 nt long are known to trigger transitivity from their targets
vsiRNAs in RISC complex act as guide molecules to target homologous viral RNA for degradation or translational repression
green fluorescent protein (GFP)-based sRNA sensor detects silencing of GFP by sRNA
siRNAs matching promoters detected with high GFP-targeting sRNAs are most likely the result of (AtRDR6, RDR6, SDE1, SGS2, AT3G49500) activity spreading siRNA production into the promoter region Nicotiana benthamiana
(ERI-1, AT3G15140) is suggested to be negative regulator of RNA silencing Caenorhabditis elegans; Schizosaccharomyces pombe; Mus musculus
DCLs mediate silencing mechanisms
amiRNAs targeting the stem-loop region (anti-164bsl) effectively reduced accumulation of (EEP1, MIR164, MIR164C, AT5G27807) Arabidopsis thaliana
artificial miRNAs introduce modified 21-nt amiRNA and amiRNA* sequences
second series of amiRNAs was designed to target three mature sister sequences of the Arabidopsis (EEP1, MIR164, MIR164C, AT5G27807) family Arabidopsis thaliana
anti-164abc amiRNA efficiently targeted primary miRNA transcript of all three (EEP1, MIR164, MIR164C, AT5G27807) family members Arabidopsis thaliana
genes participating in RNA silencing includes (AGO2, AtAGO2, AT1G31280) and SAHH Solanum tuberosum
putative loss of function of (ATDCL4, DCL4, AT5G20320) in agreement with siRNAs detected in line S39 were produced as 22 nt long molecules Arabidopsis thaliana
different terminators affect amount of siRNAs originating from the transgene
construct carrying the second intron (pRBCS::GFiP::tNOS) showed higher levels of siRNAs Nicotiana benthamiana
22 nt miRNAs trigger secondary siRNA biogenesis Glycine max
Arabidopsis (ATMYB84, MYB84, RAX3, SKI2, AT3G49690) homologue prevents formation of secondary siRNAs Arabidopsis thaliana
positive correlation between production of 22-nt (SMXL5, AT5G57130) small RNAs and (ATDCL4, DCL4, AT5G20320) leaf phenotype among individual plants supports hypothesis that silencing of (AtHSPR, SMXL4, AT4G29920) and (SMXL5, AT5G57130) may lead to anthocyanin accumulation in (ATDCL4, DCL4, AT5G20320) plants Arabidopsis thaliana
DCL2-dependent 22nt siRNAs trigger transitivity from endogenous (ATCHS, CHS, TT4, AT5G13930) locus
long npcRNAs can generate double-stranded RNAs
silencing mechanisms lead to generation of nat-siRNAs
minimal miRNA-loaded RISC in Arabidopsis may only contain (AGO1, AtAGO1, ICU9, AT1G48410) and its associated sRNA Arabidopsis thaliana
sRNA-loaded AGO complex imported back into the nucleus is smaller sRNA-loaded AGO complex Mammalia
anti-164abc-mediated silencing of (EEP1, MIR164, MIR164C, AT5G27807) is a result of cleavage of (EEP1, MIR164, MIR164C, AT5G27807) precursor transcripts Arabidopsis thaliana
miR393b* targets (ATMEMB12, MEMB12, AT5G50440) Arabidopsis thaliana
viral-associated small interfering RNAs (va-siRNAs) participate in host gene expression reprogramming
small RNAs (smRNAs) is composed of endogenous small interfering RNAs (siRNAs)
nuclear hc-siRNAs cofractionated with (AGO4, OCP11, AT2G27040)
small (s)RNAs guide RNA induced silencing complexes (RISCs)
RISCs regulate Nib gene expression Triticum aestivum
co-expressed amiRNA abolishes GFP expression by cleaving TaAAED1-GFP sensor transcript Nicotiana benthamiana
P14 silencing suppressor suppresses agroinfiltration-induced silencing response Nicotiana benthamiana
22-nt artificial microRNA (amiRNA) mediates RNA silencing Arabidopsis thaliana
(ATCHS, CHS, TT4, AT5G13930) silencing in young leaves and petioles evident in tissues in which SSU_hpCHS transgene had caused no observable effect Arabidopsis thaliana
restricted (AtSSU, GGPPS12, GGR, SSU, AT4G38460) promoter expression may explain lack of (ATCHS, CHS, TT4, AT5G13930) silencing in petioles and young leaves
vsiRNAs are subsequently incorporated into ARGONAUTE (AGO) protein
reduction of siRNAs in (ATDCL2, DCL2, AT3G03300) /3/4 mutants is inconsistent with interpretation that P4RNAs are merely longer, misprocessed siRNAs
Dicer-like (DCL) processes small RNAs (smRNAs)
(AGO7, ZIP, AT1G69440) is required for accumulation of AtlsiRNA-1 Arabidopsis thaliana
computational screen identified SPT5like Arabidopsis thaliana
RNA-induced silencing complexes (RISCs) degrade viral genomes
1758 amino acid protein with five putative domains contains WG/GW-rich region Arabidopsis thaliana
GFP-AGO1 mNES cargo approximately 95% are 21-nt-long miRNA Arabidopsis thaliana
WYMV-encoded suppressors may significantly induce and then inhibit biosynthesis of vsiRNA1
single-stranded siRNAs serve as reverse complementary guides in RNAi-induced silencing complexes
(ATDCL3, DCL3, AT3G43920) mutants contain smear of differently sized siRNAs Arabidopsis thaliana
chloroplast is devoid of RNA silencing machinery components Arabidopsis thaliana; Nicotiana benthamiana
sRNAs silence targets with complementary sequences
(AGO1, AtAGO1, ICU9, AT1G48410) cofractionated with miRNAs
computational screen identified (ATNRPD1B, DMS5, DRD3, NRPD1B, NRPE1, AT2G40030) Arabidopsis thaliana
small RNAs are typically housed and protected in RNA-inducing silencing complex (RISC)
small RNAs (smRNAs) direct control of viruses
PSR1 and PSR2 inhibit biogenesis of small RNAs
vsiRNA1 mediates cleavage of Nib mRNA Nicotiana benthamiana
vsiRNA1 is generated from WYMV NIb gene
siRNA-directed DNA methylation can result in maintained transcriptional gene silencing
knockdown approaches have limitation of possible off-target effects Chlamydomonas reinhardtii
RNA silencing is initiated in leaf tissues Arabidopsis thaliana
requirement for strong local silencing signal to mediate widespread silencing response Arabidopsis thaliana
vsiRNA biogenesis requires AGOs
Argonaute (AGO) proteins bind small RNAs
small RNA (sRNA)-guided RNA cleavage occurs in nucleus Arabidopsis thaliana
artificial miRNAs maintain structural features of endogenous precursor transcript
amiRNA technology effectively reduced (MIR159, MIR159A, AT1G73687) accumulation Arabidopsis thaliana
miR164ab levels were reduced approximately 23-fold in anti-164abc plants Arabidopsis thaliana
GFP levels are inversely correlated with siRNA abundance
RNA silencing pathways that involve miRNAs as triggers and tasiRNAs as secondary signals have targets including pentatricopeptide repeat proteins
(ATDCL3, DCL3, AT3G43920) (AtRDR2, RDR2, SMD1, AT4G11130) mutants eliminates siRNAs
endogenous small interfering RNAs (siRNAs) typically perform autosilencing DNA or transcripts corresponding to loci from which they are processed
miR393* targets different regulators through (AGO2, AtAGO2, AT1G31280) Arabidopsis thaliana
gene (AT2G16470) encodes GYF and zinc finger (CCCH-type) domain-containing protein with 11 contiguous WG/GW motifs Arabidopsis thaliana
specific RNA-binding proteins have different functions to ensure functionality of the small RNAs in gene silencing, heterochromatin formation, or mRNA post-transcriptional regulation
constructs containing an intron in the position closer to the terminator exhibited increased accumulation of siRNA Nicotiana benthamiana
RNA silencing participates in RNA-mediated antiviral defense
silencing appears to spread through transgenes
RNA silencing regulates abundance and composition of NLR transcripts
TuMV HC-Pro does not suppress vasiRNA biogenesis
2′-O-methylation of 3′ ribose of siRNAs stabilizes siRNAs
RNA polymerase V functions independently of siRNA-directed DNA methylation pathway
R-vsiR45349 transfection reduces endogenous OsFD1 transcript levels Oryza sativa
AGOs regulate host gene expression
ARGONAUTE 9 (AGO9, AT5G21150) is versatile component of RNA silencing
Argonautes (AGOs) function in eukaryotic RNA silencing pathways
construct specificity was due to target-specific silencing Hordeum vulgare
rRNA-derived vasiRNAs do not seem to direct RNA silencing in infected plants Arabidopsis thaliana
Ago-hook is found in factors implicated in AGO action
(AGO1, AtAGO1, ICU9, AT1G48410) /CMV-vasiRNA complexes do not direct silencing of host genes Arabidopsis thaliana
S-adenosyl homocysteine hydrolase virus-induced activity changes debilitate post-transcriptional RNA silencing
sRNA posttranscriptional modifications is underexplored aspect of RNA silencing
viruses suppress endogenous RNA silencing pathways
amiRNA-directed silencing of (MIR159, MIR159A, AT1G73687) and (MIR319, MIR319B, AT5G41663) confirmed by significant expression deregulation of target genes Arabidopsis thaliana
various reporter constructs were agroinfiltrated with and without the presence of viral suppressor of RNA silencing (VSR) P19 Nicotiana benthamiana
terminator usage plays a central role with respect to protecting transgenes from siRNA-directed RNA silencing
(AtRDR6, RDR6, SDE1, SGS2, AT3G49500) recruitment leads to siRNA generation
presence of introns in most cases would protect transgenes from (AtRDR6, RDR6, SDE1, SGS2, AT3G49500)
silencing can be efficiently induced by constructs lacking transcriptional termination signals
CMV 2b suppresses vasiRNA biogenesis Arabidopsis thaliana
tissue-specific expression of RNA silencing factors determines RNA silencing mechanisms
(PEG2, AT1G49290) PM cleavage was confirmed by artificially overexpressed version of siRNA854 (amiR854)
plant RISC can remain bound on target mRNAs Arabidopsis thaliana
parallel RNA analysis of transcripts (degradome analysis) identified wheat transcript cleaved and downregulated by amiRNA Triticum aestivum
Argonaute (AGO) targets host transcripts
accumulation of aberrant transcripts evokes activation of RNA silencing
dsRNA-specific RNAses, called Dicer-like (DCL) proteins process dsRNAs into viral small interfering RNA (vsiRNA)
vsiR45349 expression via (MIR319, MIR319B, AT5G41663) backbone decreases endogenous NbFD1 transcripts Nicotiana benthamiana