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protein expression

12281 relationships annotated with this phrase. Showing first 500 of 12281.
Source entity Relationship Target entity Species
(ATHSF3, ATHSFA1B, HSF3, HSFA1B, AT5G16820) did not affect (MED8, AT2G03070) protein expression Phaeodactylum tricornutum
BZR1-YFP protein level is reduced in (ARP6, ATARP6, ESD1, SUF3, AT3G33520) er-119 (ATXR3, SDG2, AT4G15180) mutant Arabidopsis thaliana
C05 and C14 proteins accumulated in inclusion bodies after IPTG induction under standard conditions Escherichia coli
GFP-CER-ZA construct was expressed in Nicotiana benthamiana leaves Nicotiana benthamiana
YN-TGB3 construction with HA tag co-delivered with bZIP60U-YC and CMV 2b Nicotiana benthamiana
YFP-SDE2 AA is detected at molecular weight c. 72 kDa Arabidopsis thaliana
oastlAB C +/− mutant shows 50% decrease in O-acetylserine(thiol)lyase isoform C protein amount Arabidopsis thaliana
zmbell10-1 mutants have substantially lower ZmBELL10 protein levels Zea mays L.
(MED8, AT2G03070) gene editing did not affect (ATHSF3, ATHSFA1B, HSF3, HSFA1B, AT5G16820) protein expression Phaeodactylum tricornutum
A2QK-7 transgenic line produced high levels of (ATHSFA2, HSFA2, AT2G26150) protein Arabidopsis thaliana
A2QK-10 transgenic line produced high levels of (ATHSFA2, HSFA2, AT2G26150) protein Arabidopsis thaliana
rNAD-ME2 had highest level of α-NAD-ME protein Kalanchoe fedtschenkoi
plant viruses are powerful tools in which to express heterologous proteins
level of (OHP, OHP1, PDE335, AT5G02120) is low in green leaves Arabidopsis thaliana
Row1::GFP is detected in total lysate Ustilago maydis
protein processing hampers production of recombinant proteins in plants Nicotiana benthamiana
WHY1-HA protein increases to peak at week 10 of plant development Arabidopsis thaliana
(BZR1, AT1G75080) protein content in (ARP6, ATARP6, ESD1, SUF3, AT3G33520) er-119 (ATXR3, SDG2, AT4G15180) SDG2pro -YFP is only slightly decreased in (ARP6, ATARP6, ESD1, SUF3, AT3G33520) er-119 (ATXR3, SDG2, AT4G15180) SDG2pro BZR1-YFP Arabidopsis thaliana
WHY1-HA protein is detected at week 6 of plant development Arabidopsis thaliana
Total proteomic analysis using tandem mass tags (TMT) revealed differential abundance of many proteins in (AtMYB1, MYB1, SRM1, AT3G09230) compared with HJ2 Oryza sativa
(MED8, AT2G03070) protein content in the mhsf3/ -GFP line was similar to MED8-GFP line Phaeodactylum tricornutum
ALMT-GFP fusion protein of 2,106 bp is larger size construct for viral vector testing Triticum aestivum
(AHA1, HA1, OST2, PMA, AT2G18960) and (AHA2, AtHA2, HA2, PMA2, AT4G30190) are two highly expressed proteins Arabidopsis thaliana
Agrobacterium-mediated transient gene expression system was applied to set out protein expression in cucumber Cucumis sativus
deletion of Slr1064 did not affect amount of Gap2 protein under autotrophic and mixotrophic conditions Synechocystis sp. PCC 6803
YFP-SDE2 transgenic plants express SILENCING DEFECTIVE 2 (SDE2) Arabidopsis thaliana
deletion of Slr1064 does not affect total abundance of Gap2 protein Synechocystis sp. PCC 6803
rhd3-8 mutant lacks detectable (GOM8, RHD3, AT3G13870) protein Arabidopsis thaliana
expression of full-length YFP-tagged (ATCNGC2, CNGC2, DND1, AT5G15410) (ATCNGC4, CNGC4, DND2, HLM1, AT5G54250) and (ATCNGC11, CNGC11, AT2G46440) /12 is very comparable across constructs Nicotiana benthamiana
oastlBC A +/− mutant shows gene dose effect on protein and activity levels Arabidopsis thaliana
cRNAs encoding the samples mentioned previously were co-injected in Xenopus oocytes Xenopus laevis
A2QK-12 transgenic line produced low levels of (ATHSFA2, HSFA2, AT2G26150) protein Arabidopsis thaliana
full length Bg_9562 is expressed in R. solanacearum (F1C1 + Bg_9562) Solanum lycopersicum
C06 and C15 proteins could be natively extracted as soluble proteins Escherichia coli
SRN1 H63A/H114A/ΔSP protein was isolated as catalytically dead control Pichia pastoris
synthetic OSC10 gene (SnOSC10) was created and expressed in yeast cells Pichia pastoris
A1AT expression indicates full-length A1AT
silencing NlVg decreased level of NlVgC
Strep A1AT was expressed in Nicotiana benthamiana wild type Nicotiana benthamiana
three transgenic lines had considerably greater (ATHSF3, ATHSFA1B, HSF3, HSFA1B, AT5G16820) protein levels Phaeodactylum tricornutum
(LIP2, LIP2p1, AT4G31050) RNAi plants show very low (LIP2, LIP2p1, AT4G31050) contents Arabidopsis thaliana
fluorescent marker proteins are expressed at high levels within 1 week
Ory s 2 translated products abundance supported by proteome data Oryza sativa
Geminivirus vectors have been developed in recent years for heterologous protein expression
C-terminal YFP fusions of RPP1-like Ler R2, R3, R4, R8 genomic constructs, and ATR1-δ51 Hpa Gw were generated for Agrobacterium tumefaciens infiltration of tobacco leaves Arabidopsis thaliana; Hyaloperonospora arabidopsidis
(BAM1, AT5G65700) and (BAM3, AT4G20270) were expressed in Escherichia coli Escherichia coli
N. lugens-secreted mucin-like protein (NlMLP) is highly expressed in salivary glands Nilaparvata lugens
BSMV system has been used for virus-mediated overexpression (VOX) of a small protein such as a fungal toxin (ToxA, 534-bp cDNA, encoding a protein of 19.7 kD)
(AHA1, HA1, OST2, PMA, AT2G18960) YCE and YNE (ATDDM1, CHA1, CHR01, CHR1, DDM1, SOM1, SOM4, AT5G66750) constructs were transiently expressed in Nicotiana benthamiana leaves Nicotiana benthamiana
TrxBC34S protein was detected in SBC34S cells Synechocystis
expression in Escherichia coli BL21(DE3) cells was induced by addition of isopropyl-β-D-thiogalactoside (IPTG) Escherichia coli
HXK family proteins were subcloned as C-terminal GFP fusion proteins
artificial AVR variants were more abundant compared to natural AVR Nicotiana benthamiana
YCE (ATSYP22, ATVAM3, SGR3, SYP22, VAM3, AT5G46860) and YNE (ATVAMP711, VAMP711, AT4G32150) constructs were transiently expressed in Nicotiana benthamiana leaves Nicotiana benthamiana
binary vector encoding full-length A1AT was used to stably transform wild-type (Columbia-0 [Col-0]), (ATHEX2, HEXO1, AT3G55260) (ATHEX1, HEXO3, AT1G65590) and plants Arabidopsis thaliana
wild-type Kalanchoe fedtschenkoi possessed clear band of (PPDK, AT4G15530) protein Kalanchoe fedtschenkoi
NlMLP protein was detected in leaves infected by strains in OD 0.01 or over Nicotiana benthamiana
barley HvCslJ gene was expressed in heterologous host N. benthamiana Nicotiana benthamiana
MSI1:GFP fusion protein expressed in MSI1:GFP transgenic plants
INP1-YFP expressed from the (ARLIM15, ATDMC1, DMC1, AT3G22880) promoter exhibited much stronger fluorescence and had a very similar pattern of protein localization at the tetrad stage compared to (INP1, AT4G22600) -YFP driven by the native promoter
TrxB mutant version was properly expressed in SBC34S cells Synechocystis
pTaNAC69–CELD plasmid contains TaNAC69-1 coding region
CP106 AVR was allowed to accumulate prior to Rx1 expression Nicotiana benthamiana
plant viral-based transient expression system was used for recombinant expression of human A1AT
T-DNA insertion studies (136 reporting protein abundance) 80% showed no protein expression Arabidopsis thaliana
6xHis tag was detected in TrxBC34S protein Synechocystis
ZmRCAα and ZmRCAβ do encode (RCA, AT2G39730) proteins Zea mays
FLAG-tagged full-length (ADO1, FKL2, LKP1, ZTL, AT5G57360) can be expressed in HEK293T cells Homo sapiens
recombinant protein encoded by (ATCAD7, CAD7, CHR, ELI3, ELI3-1, AT4G37980) expressed as C-terminal His-tagged protein Escherichia coli
H3-H4 construct accumulated to lower levels in the cell than the complete CC Nicotiana benthamiana
(ATCCS, AtHMP03, CCS, AT1G12520) mutant line was the only line where (AtSOD1, CSD1, SOD1, AT1G08830) and (AtSOD2, CSD2, CZSOD2, SOD2, AT2G28190) expression affected Arabidopsis thaliana
(ATPUB9, PUB9, AT3G07360) is expressed in nearly all tissue types except leaves Arabidopsis thaliana
(ADG1, APS1, AT5G48300) protein was confirmed to be present in lines carrying the (ADG1, APS1, AT5G48300) construct Arabidopsis thaliana
T-DNA insertion studies (136 reporting protein abundance) one in five showed no effect on or reduced protein abundance or truncated protein product Arabidopsis thaliana
Trx-2-pET23b plasmid was transformed into Escherichia coli (BLH9, BLR, HB-6, LSN, PNY, RPL, VAN, AT5G02030) (DE3)pLysS Escherichia coli
two rNAD-ME lines displayed substantial reductions in the α-subunit Kalanchoë fedtschenkoi
four-RNA system allows expression of two different proteins in planta
AS2-YFP accumulation continues to rise during 48 h incubation Arabidopsis thaliana
(FAD4, FADA, AT4G27030) rapidly diminished after being highly abundant at 8 h post-induction Saccharomyces cerevisiae
pMRR13 plants have the highest level GFP accumulation (42% TSP) in leaves
Diffuse INP1-YFP fluorescence first appears in MMC
MMC prior to cytokinesis shows low levels of diffuse YFP fluorescence
reduced incidence of pollen tube phenotypes upon co-expression of PI4Kβ1:EYFP and PIP5K5:CFP K497A was not due to low expression of either construct
dCRY of Drosophila co-expressed in HEK293 cells Drosophila melanogaster
PrrnLT7g10 yields high levels of NPTII (23%, pHK40)
protein levels of PM3A HR -β, -γ, and -δ were similar to original PM3A HR Nicotiana benthamiana
gfp-PEL3 fusion protein is expressed in pel3-11 trichomes Arabidopsis thaliana
6xHis tag was detected exclusively in SBC34S strain Synechocystis
Western blot analysis showed efficient heterologous expression of all proteins Saccharomyces cerevisiae
Arabidopsis thaliana hypocotyl cells accumulate eGFP fusion of (LTI6b, RCI2B, AT3G05890) ( –eGFP) Arabidopsis thaliana
ELONGATED HYPOCOTYL 5 (HY5, TED 5, AT5G11260) coding sequence was PCR-amplified and cloned into pDEST15 vector
cell-free expression can be automated
(ATCBL1, CBL1, SCABP5, AT4G17615) cloned into pGEX4T-1 vector
transplastomic pMRR20 and pMRR21 leaves accumulated relatively low (1% total soluble protein [TSP]) and high levels (9.1% TSP) of NPTII
GFP in the roots accumulated to very low levels, 0.14% TSP is similar to root expression of the pAI4 construct (0.13% TSP)
pYES2-BRI1:HA is used for expressing BRASSINOSTEROID INSENSITIVE 1 (ATBRI1, BIN1, BRI1, CBB2, DWF2, AT4G39400)
Arabidopsis has five cytoplasmic (HSP70, AT4G16660) forms Arabidopsis thaliana
CP106 AVR is less abundant in plants without Rx1 Nicotiana benthamiana
myxothiazol (Myxo) treatment slightly decreased chloroplast AtpC amount
transplastomic pMRR20 and pMRR21 leaves accumulated comparably high levels of GFP accumulation from the second ORF, 24% and 31% TSP, respectively
recombinant Arabidopsis farnesylcysteine lyase (FCLY, AT5G63910) is expressed in Spodoptera frugiperda (Sf9) cells Spodoptera frugiperda
all constructs accumulated ZED1-HA protein Arabidopsis thaliana
Agrobacterium-based in planta expression often results in uncontrolled overproduction of proteins
GFP in the roots accumulated to very low levels, 0.14% TSP
E. coli BL21(DE3)RIL culture induced with 1 mM IPTG Escherichia coli
NLR protein (RPP7, AT1G58602) co-expressed with immune activating allele of RPW8 protein
protein accumulation from the second ORF is independent from the first ORF
myxothiazol (Myxo) treatment did not consistently change (ATPUMP1, ATUCP1, PUMP1, UCP, UCP1, AT3G54110) amount
pYFP::MYC1 vector allows expression of VvMYC1 and Yellow Fluorescent Protein (YFP) fusion protein
neo gene expressed from L T7g10 leader yields high levels of recombinant protein
acs2-2 RR fruits show fourfold lower (ACS2, AT-ACC2, AT1G01480) protein level Solanum lycopersicum
expression of marker proteins increases over time Nicotiana tabacum; Lilium
expression of membrane proteins in their native environment should lead to active transporters
pONAC131–CELD plasmid contains ONAC131 coding region
reduced incidence of pollen tube phenotypes upon co-expression of PI4Kβ1:EYFP K864A and PIP5K5:mCherry was not due to low expression of either construct Nicotiana tabacum
CP106 AVR protein remained undetected in Rx1 plants Nicotiana benthamiana
Nicotiana benthamiana is used for transient co-expression assays Nicotiana benthamiana
exchange of resistance-increasing and resistance-reducing residues did not affect protein abundance Nicotiana benthamiana
Strep-tagged (AtCPK21, CPK21, AT4G04720) is expressed in Arabidopsis thaliana Arabidopsis thaliana
(anac021, ANAC022, NAC1, AT1G56010) protein levels were increased in NAC1-OE hairy roots Salvia miltiorrhiza
full-length PSRP7 polyprotein appears to be a major species in Setaria Setaria viridis
shared property of five AVRPM3 A2/F2 variants is overabundance Nicotiana benthamiana
myc:PDX proteins are confirmed to be expressed in respective transgenic backgrounds Arabidopsis thaliana
co-expression experiment was repeated in onion epidermal cells by transient particle bombardment assays Allium cepa
full-length PSRP7 polyprotein in Setaria differs from that reported for Chlamydomonas Setaria viridis; Chlamydomonas
Rx1 plants constitutively expressing Rx1 agroinfiltrated with Dex::CP106 AVR construct only trace levels of CP were detected upon Dex/estradiol application Nicotiana benthamiana
Gh_A10G2048 overexpression in Arabidopsis significantly increases nsLTP protein content Arabidopsis thaliana
coding region of ZmMYB138 was subcloned into pGADT7 vector Zea mays
12 h of 4°C treatment drastically inhibits (112A-2A, EMB30, GN, GNOM, MIZ2, VAN7, AT1G13980) expression Arabidopsis thaliana
antimycin A (AA) treatment resulted in variable changes in (ATPUMP1, ATUCP1, PUMP1, UCP, UCP1, AT3G54110) amount
CP106 AVR did accumulate in presence of Rx1-NES or Rx1-NLS or in combination with Rx1-NES + Rx1-NLS Nicotiana benthamiana
BnaNAC60 and BnaNAC60ΔTM were transiently expressed in oilseed rape protoplasts Brassica napus
oligomycin (OLIGO) treatment for 12 h dramatically increased chloroplast AtpC subunit amount
DpCRYs of Monarch butterfly co-expressed in HEK293 cells Danaus plexippus
casein kinase 2 (CK2) is ubiquitous kinase
L116Y variant had highest protein level Nicotiana benthamiana
PM3A HR -α was less abundant protein abundance Nicotiana benthamiana
Nicotiana benthamiana remains an excellent heterologous expression system to study proteins from various organisms (plants, microbes and animals) Nicotiana benthamiana
proteomic approach in plants has been employed to study alterations in cellular protein content
different sensitivity to heat stress explains different proteomes in roots versus leaves, grains, or fruits
(ATVHA-C, DET3, AT1G12840) protein tagged with 3× HA was transiently expressed in Nicotiana benthamiana leaves by agroinfiltration Nicotiana benthamiana
OsMTD2-MYC and SPL11-HA fusion proteins were transiently co-expressed in tobacco leaves by agroinfiltration Nicotiana tabacum
entire FLU protein could not be expressed in soluble form
cell-free expression of plastidic transporters in the presence of added detergents and lipids yields highly active transporter proteins
EGFP and EGFP-OsPIP1;3 fusion protein was properly expressed within cells Homo sapiens
abundance of amino acid biosynthetic enzymes is dynamically altered (PCK2, PEPCK, AT5G65690) mutant
GFP in the leaves of plants in this study was >10% of TSP
region α contributes to protein abundance Nicotiana benthamiana
chimeric phS:yfp gene allows for expression of fusion protein with C terminus of pherophorin-S fused to yellow fluorescent protein (YFP, mVenus) Volvox carteri
40 independent transgenic rice lines were selected for expression of GluA2 or mGluA2 Oryza sativa
BFA res. line retains both expression and subcellular localization of (112A-2A, EMB30, GN, GNOM, MIZ2, VAN7, AT1G13980) after cold treatment Arabidopsis thaliana
transient transformation of plant tissues generates recombinant proteins
SlDXS1 and SlDXS2 sequences without transit peptide cloned into pQE60 expression vector
mislocalized Rx1 variants alone and in combination permit CP accumulation Nicotiana benthamiana
fluorescence protein-tagged ethylene receptors is detectable in Agrobacterium-infiltrated tobacco leaf cells Nicotiana tabacum
YFP–FIS1B Δ166–167 was expressed via 35S promoter-driven construct Nicotiana tabacum
myxothiazol (MYXO) treatment in three independent experiments consistently reduced (ATPUMP1, ATUCP1, PUMP1, UCP, UCP1, AT3G54110) amount in alternative oxidase (AOX) overexpressor and knockdown plants
pGEX–5X-1 with Sal I – Not I fragments of PX-1 yields GST–PX-1
(ATHAK5, HAK5, AT4G13420) was not functionally expressed in yeast Arabidopsis thaliana
24 peach genes encode small GTPases Prunus persica
SCP1:gfp plants have strikingly low GFP protein levels of 0.02–0.06% of total soluble proteins Oryza sativa
(ABA1, ATABA1, ATZEP, IBS3, LOS6, NPQ2, ZEP, AT5G67030) protein from SO was detected by SDS-PAGE and immunoblot assay Escherichia coli
rice U-box proteins were expressed with GST-tags in Escherichia coli Oryza sativa; Escherichia coli
C323S mutant of Arabidopsis (FHY2, FRE1, HY8, PHYA, AT1G09570) coexpressed with wild-type (FHY2, FRE1, HY8, PHYA, AT1G09570) Arabidopsis thaliana
Suppressed hybrids A3-2, A5-1, A10-1, A14-1, A16-1 do not contain detectable HT-M polypeptides Nicotiana plumbaginifolia; Nicotiana alata
(ATUCP2, PUMP2, UCP2, AT5G58970) protein reaches only 0.02% of the mitochondrial proteins
SlTPR1-CFP and NR-YFP fusion proteins transiently expressed in onion epidermal cells Allium cepa
(AGC2, AGC2-1, AtOXI1, OXI1, AT3G25250) overexpression lines may have lower or higher OXI1 protein levels compared to wild type Arabidopsis thaliana
SGC-3xMYC and TLP-SmRSGFP were co-expressed transiently in Nicotiana benthamiana leaves Nicotiana benthamiana
Western blot analysis was used to compare protein amount of (ATPUMP1, ATUCP1, PUMP1, UCP, UCP1, AT3G54110)
YFP fusion proteins were expressed at expected molecular masses Nicotiana tabacum
marker proteins will have obtained steady-state level plant cells
PGD1:gfp plants have high GFP protein levels comparable to OsCc1:gfp, Act1:gfp, and ZmUbi1:gfp plants Oryza sativa
pEG202 and pJG4.5 plasmids generate AD-TF construct
GST-HY5 constructs generated as described previously protein construct
(ARA8, ATPCS1, CAD1, PCS1, AT5G44070) and (ATPCS2, PCS2, AT1G03980) proteins were expressed with C-terminal HA tag Arabidopsis thaliana
mutation of Complex I associates with increase in alternative oxidase (AOX) amount
marker protein expression examined at 3 hours after bombardment Nicotiana tabacum; Lilium
N- and C-terminal split-YFP fusion proteins were properly expressed in tobacco cells Nicotiana tabacum
fusion of intact (ATRH1, RH1, AT4G15850) to GFP able to obtain fluorescent transformants Chlamydomonas reinhardtii
GFP- (ICR1, RIP1, AT1G17140) produces major band about 70-kD Arabidopsis thaliana
transformed onion epidermal cells were incubated in dark at 28°C Oryza sativa
transgenic strain expressing recombinant His-tagged FcpA protein was created recombinant His-tagged FcpA protein expression Phaeodactylum tricornutum
three chimeric proteins, K50-C, R95-C and K124-C were stably expressed in transgenic Arabidopsis Arabidopsis thaliana
nYFP- and cYFP-fusion proteins were detected at similar levels in protoplasts transfected with AvrPto-nYFP and AvrPto Y89D-nYFP Arabidopsis thaliana
(AtSOD1, CSD1, SOD1, AT1G08830) and (AtSOD2, CSD2, CZSOD2, SOD2, AT2G28190) protein levels are reduced in ga1-3 quadruple-DELLA mutant Arabidopsis thaliana
cells harvested 4 h after induction Escherichia coli
(ATKTI1, AtKTI4, KTI1, AT1G73260) overexpression lines S8, S13, and S16 express functional, detectable proteins Arabidopsis thaliana
GFP–AtCASP or GFP–GC1 expressed with ST–mRFP and inducible Sar1-GTP–YFP NII tobacco plants Nicotiana tabacum
GsCRCK1a/1b/1c/1d-Cub fusion proteins were normally expressed yeast cells
Synechocystis PCC6803 strain was used as heterologous expression system for functional definition of Phat3_J9316 acyl-ACP Δ9-desaturase Synechocystis PCC6803
nodular-compact and fast-growing oil palm calli show significant differences in protein patterns and content Elaeis guineensis
fluorescence protein-tagged ethylene receptors failed to express in Arabidopsis cell culture protoplasts Arabidopsis thaliana
version 2 of RH1–GFP construct produced eight fluorescent transformants Chlamydomonas reinhardtii
ubiquitin–S subunit fusion approximately 10% of soluble Escherichia coli
recombinant cryptochrome proteins were produced in insect cell cultures
vector pET16b purchased from Novagen (Madison, USA)
Escherichia coli strain BL21(DE3)/pLysS containing pMM1311 was grown for 48 h at room temperature in ZY autoinduction medium Escherichia coli
recombinant (ATPAO2, PAO2, AT2G43020) and (ATPAO4, PAO4, AT1G65840) proteins have apparent molecular weight of ∼54 500 Da Arabidopsis thaliana
LexA-HY5 protein could not be stably expressed stable expression
YFP–FIS1A Δ167–170 was expressed via 35S promoter-driven construct Nicotiana tabacum
R1G1B:gfp plants have GFP protein levels ranging from 0.11% to 0.34% of total soluble proteins Oryza sativa
(ATTSPO, TSPO, AT2G47770) was heterologously expressed in Saccharomyces cerevisiae Arabidopsis thaliana; Saccharomyces cerevisiae
two genetically encoded inhibitors affect organelle movement several days after expression higher plants
HvDRF1:GFP fusion construct resulted in no detectable GFP fluorescence Hordeum vulgare
(HSP60, HSP60-3B, AT3G23990) SrUCPA, and SrAOX are highly expressed in stamens
FPN3-FLAG is expressed in yeast cells Saccharomyces cerevisiae
pET16b-ETR1 was transformed into Escherichia coli strain C43 (DE3) Escherichia coli
Ntann12 is not expressed in aerial part tobacco
E. coli transformed with pLEV1, pL335V, and pLEVUbS produced large amounts of tobacco L subunits Escherichia coli; Nicotiana tabacum
unique functions of roots explains different proteomes in roots versus leaves, grains, or fruits
(ATXTH14, XTH14, XTR9, AT4G25820) and (ATXTH26, XTH26, AT4G28850) were recombinantly produced in yeast Pichia pastoris Pichia pastoris
VvGST1, VvGST2, VvGST3, VvGST4, and VvGST5 were expressed in Escherichia coli Vitis vinifera; Escherichia coli
pEG202 and pJG4.5 plasmids generate LexA-HY5 construct
YFP–FIS1A/B TMD+CE (C-terminus including TMD and the adjacent C-terminal end) was expressed via 35S promoter-driven construct Nicotiana tabacum
NbDER:GFP was expressed in N. benthamiana leaves by agroinfiltration Nicotiana benthamiana
γ-secretase subunits and marker proteins were introduced simultaneously into Arabidopsis thaliana protoplasts Arabidopsis thaliana
ACD6-Col-0 was transiently expressed in human embryonic kidney 293 (HEK293) cells Homo sapiens
pUbi-HK5 is expression construct
(SPPA, SPPA1, AT1G73990) mutants are not constitutively expressing (ELIP, ELIP1, AT3G22840) Arabidopsis thaliana
wild type and MINELESS seeds showed no clear differences in myrosinase-binding protein (MBP) expression Brassica napus
pAct-HK5 is expression construct
Isopropyl β-D-1-thiogalactopyranoside (IPTG) is added to culture Escherichia coli
different bands in AOX immunoblots correlate with expression of different isoforms of AOX protein
amount of Rubisco activase in Δ43 and rwt46 plants is reduced compared with wild type Arabidopsis thaliana
sandwich construct with engineered HvDRF1 cDNA between two GFP open reading frames yielded green-fluorescing fusion product Hordeum vulgare
three different patterns of ZmCKX10 variants were overexpressed in Pichia pastoris Zea mays; Pichia pastoris
perturbations to translational processing significantly constrain production of foreign Rubiscos in tobacco plastids Nicotiana tabacum
ER–marker–RFP fusion co-expressed with ΔTM-ETR2:GFP Nicotiana tabacum
phage T7 promoter vector pETMCSI is described in Neylon et al., 2000
(AVB1, IFL, IFL1, REV, AT5G60690) protein has abundance that is increased in (AtDRB1, DRB1, HYL1, AT1G09700) seedlings
full-length open reading frame (813 bp) of kiwifruit LCY-β was cloned in translational fusion into pET30b expression vector with c-terminal HIS 6 tag Actinidia chinensis
observed aggregation of IPT1::GFP protein could also be interpreted as overexpression artefact of the maize ubiquitin promoter Physcomitrella patens
cDNA clone encoding 6-SFT from L. perenne stubble was isolated and characterized by heterologous expression in Pichia pastoris Lolium perenne; Pichia pastoris
BjCHI1 protein is present in transplastomic tobacco lines HisBjCHI1-3 and HisBjCHI1-4 Nicotiana tabacum
(HSP60, HSP60-3B, AT3G23990) SrUCPA, and SrAOX are undetectable in other tissues
recombinant cystatins successfully expressed in E. coli Escherichia coli
removal of N-terminal 60 amino acids from Solanum lycopersicum GABA transaminase 3 (Sl GABA-T3) facilitates soluble expression Solanum lycopersicum
GSTs recovered after transient expression in N. benthamiana were shown to be intact Nicotiana benthamiana
protein levels in (AGC2, AGC2-1, AtOXI1, OXI1, AT3G25250) overexpression lines are higher in wild type Arabidopsis thaliana
35S::OXI1-YFP lines show low levels of YFP protein Arabidopsis thaliana
overexpression of gliadin in the tobacco system is good way to get closer to the native expression of prolamins Nicotiana tabacum
Arabidopsis plants stably transformed with 35SCaMV :: AtPAO5-6His /pK2GW7 construct show no detectable accumulation of recombinant (AtPAO5, PAO5, AT4G29720) protein Arabidopsis thaliana
ectopic (BASL, AT5G60880) induction at later stages showed co-expression with epidermal (ATPIN1, PIN1, AT1G73590) expression only in developing serrations
pGST-CNGC20C T560A is used for bacterial protein expression
Resultant plasmids are transformed into Escherichia coli strain BL21 (DE3) Escherichia coli
CST-BIN2 is tagged (ATSK21, BIN2, DWF12, SK21, UCU1, AT4G18710) protein
pET-28M is protein expression vector
embryo axes exhibited only one band corresponding to α isoform of GDH Medicago truncatula
LjINV1 is the major isoform in the plant Lotus japonicus
native and chimeric constructs were inserted into pINIII vector Escherichia coli
line P310 (Pγzein-BSP-LT-B::GFP) compared to line P308c (P35S-BSP-LT-B::GFP) Zea mays
consequence of promoter specificity results in TAEP of P310 from the endosperm of transgenic seed Zea mays
Ntann12 is highly expressed in root system tobacco
(MED8, AT2G03070) and (ATHSF3, ATHSFA1B, HSF3, HSFA1B, AT5G16820) protein levels in double-gene-edited algae were not affected by gene editing of the other protein Phaeodactylum tricornutum
GFP protein accumulated in C. sativus Cucumis sativus
QK mutant did not contain detectable (ATHSFA2, HSFA2, AT2G26150) protein Arabidopsis thaliana
ORFs of cystatin genes cloned into pRS300 fused to eGFP and driven by 35S promoter Nicotiana tabacum
(EBS, AT4G22140) Y155A is expressed at levels similar to fully functional EBS:FLAG
(SHL, AT4G39100) W63L/Y65A is expressed at levels similar to fully functional HA:SHL
Arabidopsis thaliana (Col-0) 35S::Lti6b-GFP is fluorescent protein expression line Arabidopsis thaliana
Escherichia coli strain BL21 (DE3) carrying pET32a-SIE141, pET32a-AtCDSP32, pET32a-NbCDSP32, pET32a-StCDSP32, or pET32a-ATH5 Escherichia coli
Ntann12 is more abundant in roots tobacco
mature pea leaves could express other isoforms of AOX protein Pisum sativum
high-throughput proteomic analysis is used to study changes in protein accumulation levels
(NDHI, ATCG01090) protein reduction was approximately 75% reduction Synechocystis 6803
(AtLEA14, LEA1, LEA14, LSR3, AT1G01470) was expressed and purified from E. coli Escherichia coli
steady-state levels of various mitochondrial polypeptides were measured in wild-type and (nMAT4, AT1G74350) plants Arabidopsis thaliana
pEAQ-HT vector enables higher expression of proteins Nicotiana benthamiana
CRYs exhibit dark activity regulating proteome (174 genes) Arabidopsis thaliana
full-length complementary DNA (cDNA) represented by SGN-U575556 was expressed in E. coli with N-terminal Flag epitope tag Solanum lycopersicum
genetic constructs coding for γ-secretase subunits fused with GFP were introduced into Arabidopsis thaliana leaf mesophyll protoplasts Arabidopsis thaliana
proteomics data identified 11.2% of TFs and TRs as differentially expressed Chlamydomonas
(AtPHOT2, NPL1, PHOT2, AT5G58140) is absent under non-inducing conditions Nicotiana benthamiana
native tobacco S subunit expressed in E. coli showed solubility similar to ubiquitin–S subunit fusion Escherichia coli; Nicotiana tabacum
(ATGSTF5, ATGSTF8, GST6, GSTF8, AT2G47730) splice variant without a plastidic targeting peptide is major form in vivo Arabidopsis thaliana
purified recombinant protein from Escherichia coli showed the presence of protein of molecular mass 57 kDa Escherichia coli
D1 protein level shows no obvious difference among tested lines under normal condition Solanum lycopersicum
pGEX4T1:PHT1;4-C terminus is protein expression vector
thalli of 3-week-old Mp pbla-1 /pro 35S ::Mp PBLa-cit were infiltrated with Agrobacterium tumefaciens carrying constructs Marchantia polymorpha
pB7FWG2.0 p35S::GLR3.3D 543G -eGFP encodes (ATGLR3.3, GLR3.3, AT1G42540) D543G-eGFP fusion protein
ASY1-FL construct generated previously (ASY1, ATASY1, AT1G67370) protein expression Arabidopsis thaliana
(AtPUB22, PUB22, AT3G52450) (AtPUB23, PUB23, AT2G35930) (PUB24, AT3G11840) triple mutant shows unchanged (ATMAPK3, ATMPK3, MPK3, AT3G45640) protein abundance Arabidopsis thaliana
pHBT-CNGC20-FLAG is used for protein expression
HisMBP-ASY1 1-300 construct generated previously (ASY1, ATASY1, AT1G67370) N-terminal domain expression Arabidopsis thaliana
clones were transformed into S. cerevisiae strain WΔ6 Saccharomyces cerevisiae
(AtSOD1, CSD1, SOD1, AT1G08830) and (AtSOD2, CSD2, CZSOD2, SOD2, AT2G28190) protein levels are increased in ga1-3 Arabidopsis thaliana
double mutant H330F/H454F showed similar expression intensities as WT (SLAH3, AT5G24030) or H330K/H454K double mutant Xenopus laevis
33 proteins (root hair-specific proteins) are referred to as root hair-specific proteins Arabidopsis thaliana
'omics' approaches investigated at protein expression level Citrus sinensis
RPX-GFP fusion protein expressed in transgenic Arabidopsis Arabidopsis thaliana
pER8-Gateway-3Flag is used for inducible expression of protein fusions to 3xFLAG tag
(PFU3, UBC26, AT1G53025) (a homolog of human hKIAA1734) was impossible to induce expression of under the various conditions tested Arabidopsis thaliana; Escherichia coli
E2 genes were cloned into plant expression vector Arabidopsis thaliana
all plant lines express NPTII protein to similar levels Nicotiana tabacum
re-introduced tobacco L 8 S 8 Rubiscos examined in this study were abundantly expressed Nicotiana tabacum
level of (ATPAP10, PAP10, PUP1, AT2G16430) protein in SR– and R– is much higher than level of (ATPAP10, PAP10, PUP1, AT2G16430) protein in SR+ or R+ Arabidopsis thaliana
MT FRET-based metal-binding sensor was expressed in cytoplasm of Chlamydomonas reinhardtii Chlamydomonas reinhardtii
immunoblot analysis confirmed expression of CsCCD2-thioredoxin and ALDH-thioredoxin fusion proteins Escherichia coli
(ATVAMP711, VAMP711, AT4G32150) was transferred into RS72 yeast strain expressing heterologous (AHA2, AtHA2, HA2, PMA2, AT4G30190) Saccharomyces cerevisiae
MINELESS seeds lacked 35 kDa isoform of epithiospecifier protein (AESP, AESP1, ESP, RSW4, AT4G22970) Brassica napus
fusion protein is expressed in native genomic context Physcomitrella patens
maco2 knockout line shows significant decrease in mACO2 protein abundance Arabidopsis thaliana
four-component BSMV system demonstrates the ability of overexpress genes of larger size within 7 d post infection (dpi)
Trx-1-pET23b plasmid was transformed into Escherichia coli (BLH9, BLR, HB-6, LSN, PNY, RPL, VAN, AT5G02030) (DE3)pLysS Escherichia coli
CNF1-YFP and GFP-CNF1 fusion proteins were efficiently expressed in Nicotiana benthamiana Nicotiana benthamiana
maize Ac transposase (TPase) is expressed in cells Arabidopsis thaliana
A total of 17 Arabidopsis E2s were cloned into pET28a vector Arabidopsis thaliana
proteins expressed in plants via agroinfiltration are produced in large amounts Arabidopsis thaliana
pNB1YN-CNGC20 is used for oocyte expression
Xenopus oocytes injected with cRNAs or water Xenopus laevis
TPR and PPR proteins when expressed as recombinant protein in E. coli are often insoluble
levels of (ATPAP10, PAP10, PUP1, AT2G16430) protein on P+ medium are similar between WT and ein2 but are higher in (AtCTR1, CTR1, SIS1, AT5G03730) mutant Arabidopsis thaliana
protoplasts were co-transformed with DNA constructs encoding fusion proteins of red fluorescent protein (RFP) with sulphite reductase (SiR:RFP) or RFP with a chloroplast transcription-stimulation factor (STF:RFP) Nicotiana benthamiana
altered LeCDJ1 expression affects D1 protein level Solanum lycopersicum
maize SPS B isoform was expressed in Escherichia coli Zea mays; Escherichia coli
Tobacco mosaic virus-based magnICON vector (pICHα26211) was used for transient expression
SDE2-GFP transgenic plants show 63 kDa band Arabidopsis thaliana
WHY1-HA protein declines at week 8 of plant development Arabidopsis thaliana
full-length (ESV1, AT1G42430) encodes entire (ESV1, AT1G42430) sequence Arabidopsis thaliana
truncated version of the cDNA was expressed in Escherichia coli Escherichia coli
minovincine 19-O-acetyltransferase (MAT) is inactive when expressed in planta plant tissue Catharanthus roseus
minovincine 19-O-acetyltransferase (MAT) is inactive when expressed in yeast Catharanthus roseus; Saccharomyces cerevisiae
plastid proteome of Silene noctiflora was examined for divergent (CLPP1, PCLPP, ATCG00670) expression and function Silene noctiflora
mutated (ATRBOHA, RBOHA, AT5G07390) -B, -E and -G do not affect level of expression
HIS-MBP-PHGAP1 is tagged (PHGAP1, REN2, AT5G12150) protein
pAct-ARK-440-1148 is expression construct
ThrRS–dTP deletion constructs contains GFP fusion protein
NlMLP without the signal peptide was transiently expressed in rice protoplasts Oryza sativa
GST–ThrRS–dTP(2–60) fusion protein accumulated in inclusion bodies Escherichia coli
pHBT-CNGC19-nYFP is used for protein expression
pRI201-AN is expression vector
PIF4-GST fusion protein expression was induced by isopropyl β-D-1-thiogalactopyranoside (IPTG) Escherichia coli
mass spectrometric data on SPS protein abundance in leaves suggest (ATSPS2F, KNS2, SPS1, SPS2F, SPSA2, AT5G11110) is the dominant isoform in leaves with respect to protein levels Arabidopsis thaliana
pHBT-CNGC20N-FLAG is used for protein expression
HIS-MBP-PHGAP1(8A) is phosphorylation-deficient (PHGAP1, REN2, AT5G12150) mutant
pMAL-BAK1 CD-HA is used for protein expression
pGST-CNGC20C is used for bacterial protein expression
HIS-MBP-PHGAP2(1-420) is truncated (PHGAP2, REN3, AT5G19390) protein
NB-Bem and ND-Cdc proteins express at similar levels Arabidopsis thaliana
GFP1-10 is expressed inside recipient cells
AvrPto expression did not reduce MAPK protein accumulation Arabidopsis thaliana
pNB1YFP-CNGC19 is used for oocyte expression
pAct-HK5-1-631 is expression construct
all four phospho-dead mutations did not significantly alter (ABCG36, ATABCG36, ATPDR8, PDR8, PEN3, AT1G59870) expression or PM location Arabidopsis thaliana
(HCAR, AT1G04620) (Hydroxymethylchlorophyll a reductase) was expressed in Escherichia coli Escherichia coli
soluble protein of (UBC32, AT3G17000) was obtained when transmembrane domain was deleted Arabidopsis thaliana
transgenic protein was not detected by antibody-based analyses Solanum lycopersicum
eGFP is detected in pGW1 (PPR10-atpH), pGW8 (HCF152-petB), pGW9 (CRR2-ndhB) and pGW10 (RPL2, uL2Mz, ATMG00560) lines Nicotiana tabacum
pGW5 (PPR10-mutated (ATPH, ATCG00140) ) lines with mutated PPR10 binding site do not promote accumulation of eGFP Nicotiana tabacum
Both GFP-CNF1 and CNF1-YFP T2 seedlings were strongly fluorescent fluorescence Arabidopsis thaliana
large amounts of proteins expressed in plants is useful for subsequent experiments Arabidopsis thaliana
transient protein expression in plants may overcome problems with post-translational modifications Arabidopsis thaliana
heterologous (CaS, AT5G23060) expression in plants and yeast is first report
transformed protoplasts are incubated at 18°C for 16 h in the dark Arabidopsis thaliana
QSK1C-AA and QSK1C-DD were cloned into Escherichia coli BL21(DE3) expressing plasmid pETGST1a Arabidopsis thaliana; Escherichia coli
(SCPL19, SNG2, AT5G09640) transiently expressed in Nicotiana benthamiana Nicotiana benthamiana
wild-type or E46K mutant protein mostly was expressed as soluble protein Escherichia coli
BRI1-CITRINE amounts are generally not limiting Arabidopsis thaliana
live-cell marker ( (AML1, ML1, AT5G61960) pro:RCI2A-mNeonGreen) was created for epidermal cell outlines Solanum lycopersicum
WT or mutated QSK1 co-expression in tobacco does not significantly alter (ABCG36, ATABCG36, ATPDR8, PDR8, PEN3, AT1G59870) expression or location Nicotiana benthamiana
part of Tab 1 containing the PPPEW/OPR repeats was insoluble when expressed as recombinant protein in E. coli Chlamydomonas reinhardtii
2-Cys peroxiredoxin (BAS1, CYP72B1, CYP734A1, AT2G26710) was downregulated by high nitrogen availability at 21 days post-anthesis (DPA)
AtD14 G158E is not known if stably expressed in planta
ELONGATION FACTOR-Tu RECEPTOR (EFR, AT5G20480) C975 S-GFP was transiently expressed in Nicotiana benthamiana Nicotiana benthamiana
pGreen0179-pGLR3.3::gGLR3.3 encodes (ATGLR3.3, GLR3.3, AT1G42540) protein Arabidopsis thaliana
β-estradiol-inducible expression system is used to express fluorescently tagged fusion proteins Nicotiana tabacum
gain-of-function ACD6-1 variant was transiently expressed in human embryonic kidney 293 (HEK293) cells Homo sapiens
pHBT-CNGC20C T560/S617/S618/T619A-HA is used for protein expression
(ATGSTU24, GST, GSTU24, AT1G17170) fusion constructs transformed into Escherichia coli strain BL21(DE3) cells Escherichia coli
CP105 RB and CP106 AVR proteins can be first detected at 3 and 2 h post-Dex application respectively Nicotiana benthamiana
transgenic lines ( (ZAR1, AT3G50950) background) carrying V544E, H597E, and W825A/F839A variants accumulate similar amounts of (ZAR1, AT3G50950) protein Arabidopsis thaliana
Col-0 cerk1-2 mutant does not accumulate CHITIN ELICITOR RECEPTOR KINASE 1 (AtCERK1, AtLYK1, CERK1, LYK1, LYSM RLK1, AT3G21630) protein Arabidopsis thaliana
(ATMYC2, JAI1, JIN1, MYC2, RD22BP1, ZBF1, AT1G32640) D105N protein expression is not lacking inability of (ATMYC2, JAI1, JIN1, MYC2, RD22BP1, ZBF1, AT1G32640) D105N to confer 5-MT resistance Arabidopsis thaliana
wild type did not contain detectable (ATHSFA2, HSFA2, AT2G26150) protein Arabidopsis thaliana
rPPDK1 had complete absence of detectable (PPDK, AT4G15530) protein Kalanchoe fedtschenkoi
TrxB mutant version expression level was slightly higher than TrxB expression level in WT cells Synechocystis
OFP3-Flag fusion proteins were detected in transgenic plants Oryza sativa
S10-AtGONST1-3 × HA-S11 sandwich fusions were highly expressed and correctly targeted Arabidopsis thaliana
myc-AGO4 plant produces Myc-AGO4 protein at 110 kDa Arabidopsis thaliana
transcription factors can be expressed at levels as low as a single copy per cell
Most toxin B-expressing T1 and T2 seedlings showed only a weak fluorescent signal fluorescence Arabidopsis thaliana
recombinant full-length TBP is encoded by yeast Saccharomyces cerevisiae
MRK1-GFP, RAF26-GFP, and RAF39-GFP transiently expressed in Nicotiana benthamiana Nicotiana benthamiana
TaLBD41 protein was detectable under N-S conditions Triticum aestivum
A2QK-9 transgenic line produced undetectable levels of (ATHSFA2, HSFA2, AT2G26150) protein Arabidopsis thaliana
binary vectors were used to express A1AT
BZR1pro : BZR1-YFP and BES1pro : BES1-YFP show much lower protein levels in (ARP6, ATARP6, ESD1, SUF3, AT3G33520) er-119 (ATXR3, SDG2, AT4G15180) mutant ovules Arabidopsis thaliana
split mVenus fusion protein located at C-terminus of bZIP60U allows fluorescence visualization only when full-length bZIP60U-YC is expressed Nicotiana benthamiana
MAX1-1400 enzyme was expressed in yeast Oryza sativa
GFP::BRC1 protein accumulated at high levels 5 h after beginning of oestradiol induction Arabidopsis thaliana
SRN1 ΔSP fused with α-factor was expressed by P. pastoris Pichia pastoris
mBRI1-FLAG accumulates in transgenic Ws-2 seedlings Arabidopsis thaliana
full-length (GOM8, RHD3, AT3G13870) or (RL2, AT5G45160) were expressed as glutathione S-transferase fusion proteins in Escherichia coli Escherichia coli
absence of YFP fluorescence could not be ascribed to an effect of the YFPc fusion on protein expression Xenopus laevis
oastlAC B +/− mutant shows gene dose effect on protein and activity levels Arabidopsis thaliana
SRN1 H63A/ΔSP protein was obtained in sufficient amounts for biochemical analyses Pichia pastoris
N-terminal transmembrane domain of (ATL5, AT3G62690) inhibited adequate expression and nuclear localization of (ATL5, AT3G62690) in yeast Saccharomyces cerevisiae
stable apple callus lines overexpressing MdNAC5 (OL-1, OL-3, and OL-4) have markedly increased protein expression of MdNAC5 Malus domestica
SlSKOR expressing in Xenopus oocytes Solanum lycopersicum; Xenopus laevis
Proenzymes of each legumain were expressed in E. coli Escherichia coli
PtaJAM1.1 could not be correctly expressed coupled to HALO tag in vitro Populus tremula × alba
cucumber mosaic virus 2b silencing suppressor protein used to boost fluorescence signal Nicotiana benthamiana
pPICZ α A vector containing synthesized OSCs were expressed in Pichia pastoris Pichia pastoris
proteins in (AtMYB1, MYB1, SRM1, AT3G09230) included 672 downregulated and 660 upregulated proteins Oryza sativa
(BHLH039, bHLH39, ORG3, AT3G56980) and (ATPK10, CIPK15, PKS3, SIP2, SNRK3.1, AT5G01810) protein fusions were properly expressed plant cells Arabidopsis thaliana
recombinant full-length TBP is encoded by humans Homo sapiens
(AtCPK21, CPK21, AT4G04720) variants is transiently expressed in Nicotiana benthamiana Nicotiana benthamiana
CP106 AVR accumulation was not detectable on immunoblot following Dex application in plants co-expressing Rx1-nls or myr-Rx1 variants Nicotiana benthamiana
acs2-1 RR fruits show fourfold higher (ACS2, AT-ACC2, AT1G01480) protein level Solanum lycopersicum
(AtCPK21, CPK21, AT4G04720) wild-type and mutant enzymes were transiently expressed in Nicotiana benthamiana leaves Nicotiana benthamiana
each combination of AtCRL-GFP/AtCRL-Myc, AtCRLM5-GFP/AtCRLM5-Myc, or AtCRLM6-GFP/AtCRLM6-Myc were co-expressed in Nicotiana benthamiana leaves Nicotiana benthamiana
ZmDREB2A overexpression in maize protoplasts shows significantly greater ZmDREB2A protein accumulation Zea mays
Vectors pMRR13, pMRR18 and pMRR15 yielded 6.6%, 2.9% and 0.5% AAD
(AtCSP1, CSDP1, CSP1, AT4G36020) levels were lower in atcsp1-1 rosettes at normal and low temperatures Arabidopsis thaliana
tagged fusion proteins were expressed in plants for further purification Nicotiana benthamiana
UBC21–GFP was properly expressed, determined by Comas-blue staining on its protein amount Arabidopsis thaliana; Nicotiana benthamiana
tobacco leaf epidermal cells were found to be better suited for the expression of CDS fusion proteins than previously used BY2 cells Nicotiana tabacum
ND-Cdc protein expressed well Arabidopsis thaliana
His6-tagged NB-GAL H, NB-Cdc, NB C357S-Cdc, ND-GAL H and ND-Cdc proteins present in transgenic lines at comparable steady-state levels Arabidopsis thaliana
steady-state levels of complex I (NAD9, ATMG00070) and (BETA CA2, CA18, CA2, DEG12, AT5G14740) subunits varied only little between wild-type and (nMAT4, AT1G74350) plants Arabidopsis thaliana
TOP1–GFP fusion expressed in Nicotiana benthamiana Nicotiana benthamiana
expression level of YFP-XI-i-Δmotor fusion protein in (WIT1, AT5G11390) (WIT2, AT1G68910) was the same as in wild-type Arabidopsis thaliana
pMAL-BAK1 CD KM-HA is used for protein expression
GFP-STF1 and GFP-STF2 proteins were clearly detected in leaves of scions Glycine max
pAct-ARK-295-1148 is expression construct
individual wells were transformed with expression constructs Homo sapiens
Protein expression of MpSERK Y418F -mTb-Myc was even higher compared with MpSERK-mTb-Myc Marchantia polymorpha
(ATFD1, FD1, AT1G10960) and (ATFD2, FD2, FED A, AT1G60950) accumulate at different abundances
level of (AtSERPIN1, SERPIN1, AT1G47710) in wild-type plants is low Arabidopsis thaliana
NC-Cdc protein present in extracts in very low amounts when expressed either alone or with NB-Bem Arabidopsis thaliana
pHBT-SERK4-FLAG is used for protein expression
MBP-RIC1 is tagged (RIC1, AT2G33460) protein
pAct-HK5-YFP is expression construct
pB7FWG2.0 p35S::GLR3.3 wt -eGFP encodes GLR3.3-eGFP fusion protein
truncated GmPT without predicted transit peptide cloned in yeast expression vector Saccharomyces cerevisiae
Western blot analyses confirmed an increase in (ATPUB14, PUB14, AT3G54850) protein levels following infection Arabidopsis thaliana
DRM2uba has similar protein level as that of wild-type (DMT7, DRM2, AT5G14620)
NB-Bem and NB-Cdc proteins despite being transcribed from the 35S promoter are present at steady-state levels similar to native (HY3, OOP1, PHYB, AT2G18790) Arabidopsis thaliana
single-site mutants were individually expressed in yeast H1246 Saccharomyces cerevisiae
(FRO1, NDUFS4, AT5G67590) was notably reduced in (nMAT4, AT1G74350) mutants Arabidopsis thaliana
eGFP accumulation in pGW9 (CRR2-ndhB) plants is much more than in pGW1 plants (PPR10-atpH) Nicotiana tabacum
RAB-A5c[N125I] and RAB-A5c[WT] show relative abundance after 3 days of incubation with 20 μM Dex Arabidopsis thaliana
ADF8p : NTF / ACT2p : BirA transgenic line expresses nuclear targeting fusion (NTF) protein Arabidopsis thaliana
TaAAED1: GFP and amiRNA1 co-expression accumulated much less GFP fusion protein Nicotiana benthamiana
mutant proteins were purified from E. coli Escherichia coli
(APEM7, PEX4, UBC21, AT5G25760) (a homolog of yeast (AtUBC10, UBC10, AT5G53300) ) was insoluble Arabidopsis thaliana
Proteins expressed in Nicotiana benthamiana by agroinfiltration were used for those proteins that could not be induced or are insoluble when expressed in bacteria Nicotiana benthamiana; Arabidopsis thaliana
crr2-2 and sig4-10 are defective in NdhB and (NDHF, ATCG01010) accumulation, respectively
nuclear targeting fusion (NTF) protein is expressed properly in each transgenic line Arabidopsis thaliana
transient expression in tobacco is protein expression technique Nicotiana benthamiana
pAct-ARK-472-1148 is expression construct
antimycin A (AA) treatment slightly increased chloroplast AtpC amount
Variant transporters were expressed in Xenopus oocytes Xenopus laevis
antimycin A (AA) and myxothiazol (MYXO) treatments increased relative protein amount of mitochondrial (AthCF1beta, ATPB, CF1beta, PB, ATCG00480) protein across plant lines
GFP protein accumulation in chromoplasts of ripe tomatoes represents highest expression level achieved in non-green plastids to date
cop1-4 mutant expresses N-terminal part of (ATCOP1, COP1, DET340, EMB168, FUS1, AT2G32950) protein Arabidopsis thaliana
transformant ZF37 expressed truncated Ble::Gfp::∆AphVIII fusion protein Chlamydomonas reinhardtii
α-subunit was less strongly down-regulated in line rNAD-ME2 Kalanchoë fedtschenkoi
mammalian tissue culture is rapid eukaryotic protein expression system Homo sapiens
Tobacco mosaic virus, Potato virus X, and Tobacco rattle virus have successfully been designed and developed as vectors to express foreign proteins in dicotyledonous plants
ZmBELL10 overexpression lines have highly increased ZmBELL10 protein abundance Zea mays L.
mutations in Osalkbh5-1 and Osalkbh5-2 caused complete disappearance of the protein Oryza sativa L. ssp. japonica
G103R substitution (pbs1-3) accumulated poorly Nicotiana benthamiana
PaACLB1-B2 silencing results in differential expression of 345 up-regulated proteins Petunia hybrida
PaACLB1 protein abundance is significantly down-regulated in PaACLB1-B2-silenced plants Petunia hybrida
hierarchical clustering grouped proteins with similar expression patterns Oryza sativa
decreased activity in the dark in Elysia chlorotica is most likely due to lower levels of (PRK, AT1G32060) protein Elysia chlorotica
highest expressing proteins are often housekeeping or structural proteins
MtChit5 in pET28b vector was expressed as His-tagged recombinant protein Escherichia coli BL21 (DE3)
CSLF6 lines exhibit 133 kDa band Brachypodium distachyon
other phiLOV2.1-tagged virulence proteins show very weak fluorescence signals Agrobacterium tumefaciens
(ATRAPTOR1B, RAPTOR1, RAPTOR1B, AT3G08850) abundance may be low in vivo Arabidopsis thaliana
(ATCDC48, AtCDC48A, CDC48, CDC48A, AT3G09840) variants were transiently expressed as YFP fusions in leaf mesophyll protoplasts Arabidopsis thaliana
maize contains β-form (RCA, AT2G39730) polypeptides Zea mays
(AAD6, FTM1, HUP7, SAD6, AT1G43800) gene was expressed in Escherichia coli Escherichia coli
low levels of diffuse YFP fluorescence in MMC are significantly higher than background level in untransformed plants
A2Wt transgenic line produced high levels of (ATHSFA2, HSFA2, AT2G26150) protein Arabidopsis thaliana
CsCCD2-thioredoxin and ALDH-thioredoxin fusion proteins were expressed at expected molecular masses of 81.9 and 69.8 to 76.9 kD Escherichia coli
GlgC-TM protein was confirmed to be present in lines carrying the GlgC-TM construct Arabidopsis thaliana
(AAD6, FTM1, HUP7, SAD6, AT1G43800) gene was expressed in Saccharomyces cerevisiae Saccharomyces cerevisiae
rNAD-ME1 had reduction in α-subunit protein level Kalanchoe fedtschenkoi
NIP7;1 protein is expressed preferentially at high levels in tapetum compared with other floral tissues Arabidopsis thaliana
Strep A1AT was expressed in glycosylation mutant ∆XT/FT Nicotiana benthamiana
GFP-tagged (AtTCP21, CHE, TCP21, AT5G08330) can be expressed in HEK293T cells Homo sapiens
(ATDDM1, CHA1, CHR01, CHR1, DDM1, SOM1, SOM4, AT5G66750) YCE and YNE (ATVAMP711, VAMP711, AT4G32150) constructs were transiently expressed in Nicotiana benthamiana leaves Nicotiana benthamiana
13 E2 proteins belonging to nine E2 groups were successfully expressed in Escherichia coli and purified using Ni-NTA agarose Arabidopsis thaliana; Escherichia coli
cold stress alters protein expression profiles Arabidopsis thaliana
35S promoter controls expression of PdGATL1.1-EYFP fusion construct
(AAD6, FTM1, HUP7, SAD6, AT1G43800) protein was not active in heterologous expression systems Saccharomyces cerevisiae; Escherichia coli
(ATRAPTOR1B, RAPTOR1, RAPTOR1B, AT3G08850) T-DNA KO lines show 477 proteins significantly up- or down-regulated Arabidopsis thaliana
suitable virus vector for systemic expression of heterologous proteins in the recalcitrant monocotyledonous plants is currently not available
two proteins identified in (B73, CHL6, CNX, CNX1, SIR4, AT5G20990) shoot apices disappear in either kn1 or (AtSIP1, RS1, SIP1, AT1G55740) mutants Zea mays
TrxB protein was not detected in STXB mutant cells Synechocystis
constitutive promoter-driven expression of ZmSRO1d-S and ZmSRO1d-R results in low ZmSRO1d-S and ZmSRO1d-R protein levels Zea mays
22 proteins accumulated to higher levels in yl mutant compared with WT Solanum lycopersicum
SlGDSL2 protein failed to be detected in epidermis from gdsl2-b fruit Solanum lycopersicum
fluorescent fusions of FtsZ from diverse photosynthetic organisms were expressed in heterologous system
FLAG-tagged (ADO1, FKL2, LKP1, ZTL, AT5G57360) decoy can be expressed in HEK293T cells Homo sapiens
Immunoblotting with anti-His-6 antibody revealed CsUGT74AD1-thioredoxin fusion protein Escherichia coli