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protein detection

9732 relationships annotated with this phrase. Showing first 500 of 9732.
Source entity Relationship Target entity Species
signal from immunoblot analysis represents both (LHCA2*1, Lhca6, AT1G19150) and Lhca7 Chlorella ohadii
WT (wild-type Phaeodactylum tricornutum) has (MED8, AT2G03070) protein band Phaeodactylum tricornutum
immunoblot analysis detected full-length SlFERL protein in WT and absence in Slferl mutants Solanum lycopersicum
fluorescent protein fusions evaluate enzyme(s) at RNA or protein level
YN-TGB3 was not as notable in total protein sample Nicotiana benthamiana
free GFP readily observed in western blot Nicotiana benthamiana
Avr9B-like proteins from S. lycopersici and P. fuligena could be detected by western blotting Nicotiana tabacum
N-terminal RFP and C-terminal GFP protein ensure processing near N- and C-terminus can be detected by Western blots Nicotiana benthamiana
(ML3, AT5G23820) protein is detectable in (NAI2, AT3G15950) mutants Arabidopsis thaliana
(ML3, AT5G23820) protein is absent in (NAI1, AT2G22770) mutants Arabidopsis thaliana
streptavidin-FITC approach is employed for in-situ visualization of glutathionylated proteins
immunoblot analysis used antibodies against CF0 III
fucose-specific lectin Aleuria aurantia recognizes Craspedostauros australis adhesive trails Craspedostauros australis
control experiments with preimmune serum and secondary antibody alone showed no labelling of adhesive trails Craspedostauros australis
polyubiquitinated 2HA-PI4Kβ2 enables detection in immunoblot Arabidopsis thaliana
(ATHSF3, ATHSFA1B, HSF3, HSFA1B, AT5G16820) protein band could not be found in (ATHSF3, ATHSFA1B, HSF3, HSFA1B, AT5G16820) gene-edited algae Phaeodactylum tricornutum
truncated variants of FLAG-tagged PIFs facilitate detection according to previous successful experiences
immunoblot analysis confirmed accumulation of larger bZIP60U-YC and smaller bZIP60S-YC fusions Nicotiana benthamiana
JIM8 and JIM13 antibodies detect arabinogalactan protein epitopes Arabidopsis thaliana
Myc-fused sHSP22-Δ transgenic plants had myc-fused protein levels confirmed using anti-myc antibody in three independent homozygous lines Arabidopsis thaliana
primary antibodies are mouse monoclonal antibodies
SDS-PAGE gels are fixed and exposed to Hyperfilm™
radiolabeled proteins analyzed by phosphorimager Pisum sativum; Physcomitrella patens; Chlamydomonas reinhardtii
total soluble proteins were incubated with tobacco anti-PRK antibody
polypeptide profile of seed protein from Col or nramp3-4 was attested by staining with Coomassie blue Arabidopsis thaliana
hemagglutinin-STREP-tagged (ATRUB1, NEDD8, RUB1, AT1G31340) (HSN) immunoprecipitation detects (ML3, AT5G23820) in unmodified form Arabidopsis thaliana
Avr9B could not be detected in total protein samples from Nicotiana tabacum ATTAs using western blotting Nicotiana tabacum
TALE protein expression assessed by Western blot analysis Xanthomonas phaseoli pv manihotis
YFP-Sec24 fusion demonstrates accurate FP detection Nicotiana benthamiana
nrt1.12-1 and nrt1.12-2 mutants could not be detected in (NRT1.12, AT3G16180) protein Arabidopsis thaliana
YC3.6 protein accumulation confirmed by Western blot analysis Chlamydomonas reinhardtii
GFP signal was visible immediately and along whole transgenic roots Amaranthus hypochondriacus
(MED8, AT2G03070) gene-edited algae lack (MED8, AT2G03070) protein band Phaeodactylum tricornutum
TurboID fusion proteins expression confirmed by immunoblot analysis Arabidopsis thaliana
peptide antibodies against CaTrailin-2, -3 and -4 were raised against CaTrailin-2, -3 and -4 Craspedostauros australis
prevention of vacuolar protease activity enables visualization of GFP in vacuole Arabidopsis thaliana
Western blot analysis uses anti-NTRC antibody
two polypeptides of, respectively, 26.5 and 28 kDa were detected in fruit samples during development Arabidopsis thaliana
acry strain generated with CRISPR/Cas9 technique still shows small levels of aCRY protein in immunoblotting analysis Chlamydomonas reinhardtii
PVDF membrane with transferred proteins incubated with mouse α-MSI1 or mouse α-GFP antibody
loading limit of 2D gels will favor the identification of abundant proteins
anti-poly(ADP)ribose antibody detected with anti-rabbit IgG alkaline phosphatase conjugate Arabidopsis thaliana
cYFP fusion construct (HA-tagged) detected by anti-HA antibody (α-HA) Arabidopsis thaliana
anti-ML3 peptide antibody detects 18-kD (ML3, AT5G23820) protein Arabidopsis thaliana
separated proteins were blotted on Hybond-P PVDF membranes
membranes were stained with Coomassie blue
Protein extracts from Escherichia coli transformed with pET-30a expressing truncated ORF of ZmRCAα or ZmRCAβ were recognized by anti-cotton (RCA, AT2G39730) antibody Zea mays; Gossypium sp.
total soluble protein (TSP) extracts contained two signals of 40 and 52 kilodaltons
only commercially available mMDH antibody cross reacts with both isoforms ( (mMDH1, AT1G53240) (mMDH2, AT3G15020) ) Arabidopsis thaliana
(ATNCED6, NCED6, AT3G24220) could barely be detected directly by iTRAQ Arabidopsis thaliana
total protein was stained with Coomassie Brilliant Blue Arabidopsis thaliana
immunoblot analysis used antibodies against (ADL3, CF1, DL3, DRP2B, AT1G59610) β
nYFP fusion construct (myc-tag) detected by anti-c-myc antibody (α-c-myc) Arabidopsis thaliana
SAUR62-GFP and SAUR75-GFP were not detected in wild-type flowers Arabidopsis thaliana
Western blot analysis uses anti-2-Cys Prx antibody from rice Oryza sativa
proteins were visualized by Coomassie staining
(ATFLS2, FLS2, AT5G63580) is detected by αFLS2-peptide antibody Arabidopsis thaliana
Biotin tag on proteins of interest allows detection by immunoblotting with biotin antibodies
Coomassie blue staining verified equal loading of samples
HvGlyT4 antiserum detected 55-kDa protein Hordeum vulgare
Pisum sativum anti-reversibly glycosylated protein 1 (ATRGP1, RGP1, AT3G02230) antibody used to detect reversibly glycosylated protein 1 (ATRGP1, RGP1, AT3G02230) Pisum sativum
SDS–PAGE gel was analyzed using immunoblotting
gels were exposed to phosphorimaging screens
(ATFKBP12, FKBP12, FKP12, AT5G64350) in co-10 mutant plants was not altered compared with Col-0 wild type (WT) Arabidopsis thaliana
(ATFKBP12, FKBP12, FKP12, AT5G64350) protein in fk12-1 and fk12-5 mutants was almost completely absent fk12-1 and fk12-5 mutants Arabidopsis thaliana
insertion of two methionines in C-terminal end of GFP increases signal of detection
separated proteins were analyzed by immunoblotting with antibodies against YFP Arabidopsis thaliana
blots were incubated with secondary antibodies
goat anti-rabbit IgG antibody conjugated to alkaline phosphatase or horseradish peroxidase
immunoblot analysis used antibodies against (ALB3, AT2G28800)
transgenic poplars overexpressing xylanase gave a 40-kDa signal on Western blot Populus trichocarpa
40-kilodalton protein band was detected with anti-A1AT antibodies but not anti-GFP antibodies
immunostaining of transgenic plants comes from OHP1-Myc fusion proteins Arabidopsis thaliana
primary antibody-incubated membrane incubated with goat α-mouse HRP secondary antibody
Western blot analysis uses anti-TrxB antibody
proteins are blotted onto nitrocellulose
C-terminal fusion tags include FLAG peptide
anti-GFP rabbit polyclonal antibody (Molecular Probes, Leiden, The Netherlands) used at dilution of 1:5000 Arabidopsis thaliana
anti-rabbit IgG alkaline phosphatase conjugate detected with alkaline phosphatase substrate solution-blue Arabidopsis thaliana
SDS–PAGE is followed by Western blot
ferritin abundance was determined in wild-type plants and various ferritin mutant plants Arabidopsis thaliana
intercellular fluid (IF) showed no signal with strep tag-specific antibodies
three bands in range of 50 to 55 kilodaltons reacted with anti-A1AT and antistrep antibodies
immunoblotting analysis of wild-type thylakoid membranes after BN-PAGE did not result in any signal Arabidopsis thaliana
secondary antibody is anti-mouse antibody
radiolabeled proteins analyzed by X-ray film exposure Pisum sativum; Physcomitrella patens; Chlamydomonas reinhardtii
~80-kDa protein no detectable protein in homogenate and MM fractions Hordeum vulgare
protein bands cross-reacting with anti-VIPP1 antibodies were identified in protein extracts from both purified CLD and βC-plastoglobuli Dunaliella bardawil
immunoblot analysis uses antibodies against cpSecY
amount of (OHP, OHP1, PDE335, AT5G02120) in -3xHA transgenic plants was below immunological detection limit Arabidopsis thaliana
Biotin tag on proteins of interest allows detection by immunoblotting with HRP–avidin
anti-RGP1 antiserum detected protein of expected size in homogenate, MM, and DEX fractions Hordeum vulgare
different proteins derived from the same gene (isoforms) present challenge for antibody cross-reactivity
phosphorylation state of phosphoenolpyruvate carboxylase (PPC) can be measured using anti-phospho-PPC antibodies to probe immunoblots Kalanchoë fedtschenkoi
polyclonal antibody against recombinant AaXEG2, HvXYL1, AtCel1, and Tv6GAL was used to assess transgene expression Populus trichocarpa
VIPP1-His protein was detected as IPTG-inducible protein around 25- to 37-kD marker Escherichia coli
BdTHX1 antibody did not detect 6×HIS-BdTHX2 Brachypodium distachyon
bound antibodies were visualized with alkaline phosphatase
immunoblot analysis uses antibodies against (ALB3, AT2G28800)
(ABI4, ATABI4, GIN6, ISI3, SAN5, SIS5, SUN6, AT2G40220) could barely be detected directly by iTRAQ Arabidopsis thaliana
anti-α-tubulin DM1A mouse monoclonal antibody recognizes α-tubulin
antibody raised against recombinant (ATOBGC, ATOBGL, CPSAR1, EMB269, EMB3138, Obg A-2, OBGC, OBGL, AT5G18570) protein recognizes recombinant (ATOBGC, ATOBGL, CPSAR1, EMB269, EMB3138, Obg A-2, OBGC, OBGL, AT5G18570) protein Arabidopsis thaliana
intact A1AT-GFP fusion protein (approximately 80 kilodaltons) was detected when either anti-A1AT or anti-GFP antibodies were used
Western blot analysis uses anti-glutamine synthetase antibodies
higher molecular weight complexes were recognized by anti-TrxB antibody Synechocystis
SDS-PAGE separated samples subjected to immunoblot analysis
tobacco PRK-specific antibody decorated 38-kDa protein in soluble extracts of V. litorea Vaucheria litorea
tobacco PRK-specific antibody decorated 38-kDa protein in extracts of plastids isolated from E. chlorotica (starved 9 months) Elysia chlorotica
tobacco PRK-specific antibody decorated 38-kDa protein in extracts of plastids isolated from Spinacea oleracea Spinacea oleracea
HvGlyT4 Golgi marker antibody used to detect HvGlyT4 Hordeum vulgare
OsFBK1 is detected in panicle protein extracts Oryza sativa
SAUR62-GFP and SAUR75-GFP were detected in corresponding transgenic flowers Arabidopsis thaliana
low abundance proteins will probably not be identified with 35S-cysteine labeling method
gels subjected to silver staining
PVDF membrane was labeled with protein-specific antibodies
immunoblot analysis uses antibodies against (ALB3, AT2G28800)
duplicate gels were subjected to western blotting
anti-HvCSLC2 antiserum detected ~80-kDa protein enriched in PEG fraction Hordeum vulgare
anti-mouse HRP-labeled secondary antiserum was used for (TAR1, AT1G23320) detection
radiolabeled products of in vitro translation did not significantly change the detection by protein dyes
[35S] signals detected in Coomassie Blue stained and dried SDS–PAGE gels
(ABI5, AtABI5, DPBF1, GIA1, AT2G36270) could barely be detected directly by iTRAQ Arabidopsis thaliana
immunoblots performed using chemiluminescence reagents
(AtCPK21, CPK21, AT4G04720) or mutated protein variant is visualized using anti-Strep-HRP conjugate immunodetection Nicotiana benthamiana
anti-TS2 antibody detects proteins a and d Setaria viridis
antibody against cotton (RCA, AT2G39730) detects α-form (RCA, AT2G39730) polypeptide Zea mays
rNAD-ME1 had PPDK protein readily detected using immunoblotting Kalanchoe fedtschenkoi
anti-glutathione antibody exhibits low sensitivity
low sensitivity of anti-glutathione antibody greatly limits number of glutathionylated proteins detected
immunoblot analysis uses antibodies against (ALB4, ARTEMIS, STIC1, AT1G24490)
antibodies raised against CsUGT74AD1 recognized band of 51 kD Crocus sativus
polyclonal antibody revealed single band of correct size Brachypodium distachyon
protein expression analyzed by immunodetection using an anti-HA antibody (α-HA) Arabidopsis thaliana
ECL PLUS kit is used for detection of proteins
immunoblots performed using anti-FLAG M 2 primary antibody
anti-S1/NH antibody detects major protein bands of approximately 107, 40 and 27 kDa Setaria viridis
proteins were analyzed by Western blot analysis with anti-His antibody
violet-blue wavebands (400–490 nm) likely associated with protein absorption
psrp7 mutant shows species migrating between proteins a and c at low abundance Setaria viridis
antibodies specific to (ATTOC159, PPI2, TOC159, TOC160, TOC86, AT4G02510) used for detection of (ATTOC159, PPI2, TOC159, TOC160, TOC86, AT4G02510)
root apices were incubated with anti-mouse GFP antibody
WT N. benthamiana plants transiently transformed with Est::Rx1 and Dex::CP106 AVR constructs inducers were added at different time points and accumulation of CP106 AVR was determined by ELISA Nicotiana benthamiana
anti-Rubisco used for detection of Rubisco
antibody against rice OsSUT1 did not cross-react with ShSUT1 Oryza sativa; Saccharum officinarum
dried SDS-PAGE gel is analyzed by autoradiography using STORM-PhosphoImager
gel blots are probed using anti-DPE2 antibody
blots are stained by Ponceau S
primary polyclonal anti-TAR1 peptide antibody binds to (TAR1, AT1G23320) protein
virus in systemic tissues absence confirmed by immunoblotting Nicotiana benthamiana
Western blotting performed with histone H3 acetyl K9 ChIP grade antibody
(AtCPK21, CPK21, AT4G04720) protein is visualized using streptavidin-AP-conjugate immunodetection Nicotiana benthamiana
protein content has been mainly retrieved from red-edge region (680–780 nm)
recombinant polyhistidine-tagged proteins were confirmed by Western blot analysis
immunocytochemical procedure can be applied to localize proteins secreted by parasitic nematodes during all phases of infection
anti-mouse HRP-labeled secondary antiserum binds to primary polyclonal anti-TAR1 peptide antibody
lower band is (ATMAPK3, ATMPK3, MPK3, AT3G45640) Arabidopsis thaliana
CP106 AVR accumulation in stable transgenic Rx1 plants was quantified as control for this set-up Nicotiana benthamiana
PVDF membranes were reacted with primary polyclonal anti-TAR1 peptide antibody
polyclonal antiserum is suitable to detect homologous Cytc1 proteins Pisum sativum; Spinacia oleracea
antibody raised against recombinant (ATFER1, FER1, AT5G01600) protein recognized four different ferritin subunits Arabidopsis thaliana
Western blotting performed with histone H3 acetyl K9/K14 antibody
ferritin abundance was determined by Western blot analysis using antibodies raised against recombinant (ATFER1, FER1, AT5G01600) protein Arabidopsis thaliana
Cy3 profile shows image of biotinylated proteins Arabidopsis thaliana
Western blotting of PAL was carried out as described by Towbin et al. (1979)
protein amount from N. benthamiana leaves purified and immobilized (AtCPK21, CPK21, AT4G04720) variants was shown by Western blot analysis Nicotiana benthamiana
anti-biotin antibody immunoblots S-nitrosylated proteins Arabidopsis thaliana
Western analysis was carried out according to Jakob et al. protocol
antibody against rice OsSUT1 did bind to phloem cells Saccharum officinarum
canopy-based yield prediction model detected relevant wavebands associated with protein absorption
unlabelled (PCK2, PEPCK, AT5G65690) peptides are identified in wild-type and TAP-tagged (PCK2, PEPCK, AT5G65690) plants Arabidopsis thaliana
(ARA12, SBT1.7, AT5G67360) protein spots 3 and 4 having highest signal Arabidopsis thaliana
signal intensity comparison between wild type and IM Man1A / IM Man1A xIM XylT1A showed even an increase in signal intensity Chlamydomonas reinhardtii
polyclonal antibodies against the synthetic peptide CGVTLEEEKMNGLMG were used to detect (S8H, AT3G12900) in protein extracts from 14-d-old Arabidopsis seedlings by western-blot analysis Arabidopsis thaliana
other commercially available antibodies appear to have the same problems of sensitivity and specificity
anti-H+-ATPase antiserum detected ~90-kDa band in homogenate fraction Hordeum vulgare
anti-GFP is used as primary antibody
anti-DXS2 antibody is applied in Western blot assay
antibodies specific to atToc75 used for detection of atToc75
nuclei isolated from Col-0, fk12-5 and 35S: FK plants were analyzed for CO and (ATFKBP12, FKBP12, FKP12, AT5G64350) protein levels Arabidopsis thaliana
low levels of tagging are still detectable
(B73, CHL6, CNX, CNX1, SIR4, AT5G20990) a non-transgenic maize inbred line showed no signal in Western blot Zea mays
abundance of G6PDH proteins was investigated by western blotting using antibodies able to discriminate the plastidial P2-isoform Hordeum vulgare
no detectable GFP band for P309 (Pγzein-GFP) was observed, which carries the GFP with no signal peptide Zea mays
TaCM protein is detected as single band in wheat stem samples
anti-R2-HMW polyclonal antibody does not react with gliadins or low molecular weight (LMW) subunits Triticum aestivum
anti-AtREP antibody recognized both the WT version of REP present in the WT and REP/rep-2 lines and the truncated version present in the rep-1/rep-1 and rep-1/rep-2 lines Arabidopsis thaliana
protein band was clearly detected using anti-histidine (anti-HIS) and anti-GST antibodies Escherichia coli
(AtCPK21, CPK21, AT4G04720) protein from Arabidopsis thaliana is visualized using ECL-substrate detection Arabidopsis thaliana
epitope availability for mAbs determines differences in interaction of two mAb groups with plastid-localized CBP70 Zea mays
chloroplast proteins (RBCL, ATCG00490) and PC indicated the presence of chloroplast proteins in pale yellow plants
transgenic lines show no signal with S3B512 mAb Triticum aestivum
high abundance of recombinant protein enables direct monitoring of fluorescence emission of oxidized flavin bound to apoprotein
Western blotting confirmed the presence of subtilisin in soybean xylem fluid and Medicago truncatula suspension culture fluid Medicago truncatula; Glycine max
Western blot analysis performed on nitrocellulose membrane
His-tagged (MORF8, RIP1, AT3G15000) (ICR1, RIP1, AT1G17140) is detected using His probes
Rieske Fe-S protein (antibody 2) was detected by antibody 2 to Rieske Fe-S protein Spinacia oleracea
each fraction were analyzed by SDS–PAGE and protein blot Nicotiana tabacum
serum reacted with expansins (35–41 kDa) Meloidogyne incognita
Immunoblot ECL analysis used to detect protein
immunoblots were probed using antibodies against (ATPEP12, ATSYP21, PEP12, PEP12P, SYP21, AT5G16830) Arabidopsis thaliana
anti-ABP41 antibody recognizes ABP41
separated proteins stained with Coomassie Brilliant Blue
primary antibodies raised against Arabidopsis (CCT2, AT5G20890) Arabidopsis thaliana
water-washed starch fraction from T2 seeds of 3B-3-6 and 48-1-6 lines did not contain gel detectable amounts of PINA and PINB proteins Triticum aestivum
monomeric band after urea treatment excludes unspecific recognition by anti (ATPRXIIF, PRXIIF, AT3G06050) antibodies
anti-GluA antibody recognizes GluA1 and GluA2 acidic subunits Oryza sativa
inclusion bodies were not labelled by A14 antibody Triticum aestivum
Western blots were scanned at two different wavelengths Arabidopsis thaliana
Western blots probed with either Cy3-conjugated or horseradish peroxidase (HRP)-conjugated secondary antibodies revealed that PEROXIDASE 52 (PRX52, AT5G05340) was cross-linked to azido-ATP-biotin Arabidopsis thaliana
Mi-1 antibody raised against the N-terminal region of Mi-1 recognizes CC-NB-ARC protein Nicotiana benthamiana
immunoblots were probed using antibodies against γTIP Arabidopsis thaliana
ubiquitinated substrates were detected by ubiquitin antibody
in-gel staining with tetramethylbenzidine and hydrogen peroxide non-specifically stains haem and copper-containing proteins
(MORF8, RIP1, AT3G15000) /ICR1-specific polyclonal antibody recognizes 90-kD modified (MORF8, RIP1, AT3G15000) (ICR1, RIP1, AT1G17140) protein Arabidopsis thaliana
(ATPEP12, ATSYP21, PEP12, PEP12P, SYP21, AT5G16830) antibodies recognize triplet of proteins corresponding to (ATPEP12, ATSYP21, PEP12, PEP12P, SYP21, AT5G16830) Arabidopsis thaliana
plant proteins detected by Western blot analysis Arabidopsis thaliana
Mi-1 antibody raised against the N-terminal region of Mi-1 recognizes TAP-LRR fusion protein Nicotiana benthamiana
antibody against poplar GPX3.2 may recognize several GPX isoforms Pisum sativum
Western blotting monitored protein content of (ATXTH14, XTH14, XTR9, AT4G25820)
His-tagged (MORF8, RIP1, AT3G15000) (ICR1, RIP1, AT1G17140) mutant proteins is detected using His probes
9–11 mm seed extracts incubated in sample buffer without thiol reducing agents showed detection of 80 kDa and 39 kDa bands plus strong 42 kDa band Glycine max
39 kDa lower GmFAD7 protein band was detected with very low intensity but in similar amounts under all experimental conditions Glycine max
effector dilution when injected into cells prevents detection by direct immunocytology Meloidogyne incognita
BIN2:GFP and bin2-1:GFP proteins were analyzed by immunoblotting using monoclonal GFP antibody Arabidopsis thaliana
antibodies specific for SBE IIa or SBE IIb were used for immunoblotting Hordeum vulgare
RNA polymerase not detected tomato chromoplast proteome Solanum lycopersicum
anti-Mi-ASP2 antibody specifically binds to individual protein band of ∼50 kDa Meloidogyne incognita
FRB–CCT2 fusion protein is specifically detected by (AT-PHH1, ATCRY2, CRY2, FHA, PHH1, AT1G04400) antibody Arabidopsis thaliana
PLDα1 made visible by staining alkaline phosphatase activity with Bio-Rad immunoblotting kit
Atsnx2b knockout mutant shows no specific signal with AtSNX2b immunofluorescence staining Arabidopsis thaliana
seed extracts resuspended in sample buffer containing thiol reducing agents (β-MeOH or DTT) showed similar band pattern with 80 kDa and lower 39 kDa bands Glycine max
anti-UGPase antibody used in Western blot analysis
Western blotting uses anti-c-Myc antibody Arabidopsis thaliana
35S-BIN2:GFP transgenic lines exhibit GFP signals by direct Western blotting and confocal microscopy Arabidopsis thaliana
proteins on nitrocellulose membrane blotted using same conditions as described above Arabidopsis thaliana
FKBP–CCT2 fusion protein is specifically detected by (AT-PHH1, ATCRY2, CRY2, FHA, PHH1, AT1G04400) antibody Arabidopsis thaliana
Anti-peptide antibody specifically detects polypeptides with apparent molecular weights of ~9.7 kDa Nicotiana alata; Nicotiana plumbaginifolia
membrane incubated with antibody raised against castor bean PLDα1
Western blotting uses anti-HA antibody Arabidopsis thaliana
(MORF8, RIP1, AT3G15000) /ICR1-specific polyclonal antibody does not react with (ICR2, RIP2, RIP3, AT2G37080) and recombinant proteins Arabidopsis thaliana
rabbit polyclonal GFP antibody used to detect YFP and CFP proteins Nicotiana tabacum
intact sGFP418 is detectable by anti-GFP antiserum Nicotiana tabacum
anti-AtSNX2b antibodies specifically recognize AtSNX2b Arabidopsis thaliana
two groups of mAbs differ in reaction with CBP70 in immunogold electron microscopy Zea mays
serum specific to subfamily of M. incognita pectate lyases recognizes pectate lyases Meloidogyne incognita
immunolocalization was used together with fluorescence labelling Torenia fournieri
BEIIb detected by immunoblot analysis Zea mays
PsABI3-1 and PsABI3-2 are visible in agarose gels Pisum sativum
Western blots were probed with anti-UGPase antibody Arabidopsis thaliana
immunoprecipitated BIN2:GFP was analyzed by Western blot analysis
CF0-suIII (integral membrane component of ATPase) was detected by antibody to CF0-suIII Chlamydomonas reinhardtii
prehybridization performed Western blot analysis
OsARF1, OsARF4, OsARF7, OsARF10, OsARF22, OsARF23, and truncated OsARF11 are detected by western blotting Oryza sativa
GAPDH antibodies detected GAPDH bands at 150, 160, 300, 600, and 700 kDa Medicago truncatula; Pisum sativum; Vicia faba; Phaseolus vulgaris; Solanum tuberosum; Solanum lycopersicum; Nicotiana tabacum; Spinacia oleracea; Arabidopsis thaliana
Western blot used with anti-c-Myc antibody
membrane incubated with second antibody
rest of plant species analysed (Medicago, Lotus, cowpea) showed single reacting band Medicago sativa; Lotus japonicus; Vigna unguiculata
other bands of higher MW (>90 kDa) could be observed upon higher exposure of blots Glycine max
42 kDa band is coincident with upper band of leaf doublet Glycine max
immunofluorescence with anti-GFP antibodies detected GR2-GFP in cells permeabilized with Triton X-100 Nicotiana tabacum
anti-MdSDH6 serum recognizes MdSDH5 Malus domestica
NAD-SDH is detected in microsomal fraction Malus domestica
OsCAS–YFP/OsCAS K99G–HIS heterodimer is recognized by both anti-HIS antibody and anti-GFP antibody Saccharomyces cerevisiae
wheat contains three Rubisco activase (RCA, AT2G39730) cross-reacting bands Triticum aestivum
BIN2:GFP genomic construct expression resulted in no GFP signal detected by Western blotting analysis or confocal microscopy Arabidopsis thaliana
Rieske Fe-S protein (antibody 1) was detected by antibody 1 to Rieske Fe-S protein Arabidopsis thaliana; Escherichia coli
two bands in range of 39–42 kDa reacted with GmFAD7 antiserum Glycine max
two bands of 47 kDa and 60 kDa in roots varied in intensity depending on preparations Glycine max
anti-murine (ATTSPO, TSPO, AT2G47770) antibodies immunodetected by Western blot polypeptides of various sizes
anti-Ntann12 antibodies do not cross-react with protein in leaves tobacco
SrUCPA and SrAOX proteins could be identified in total protein extracts Symplocarpus racenosus
SSIIa signal totally absent in su2 mutant extract Zea mays
NAD-SDH is detected in soluble fraction Malus domestica
Rubisco activase (RCA, AT2G39730) cross-reacting bands observed in Arabidopsis thaliana leaf protein extracts Arabidopsis thaliana
horseradish peroxidase-conjugated anti-rabbit secondary antibody used at dilution 1:10,000 dilution
protein blot used anti-GFP antibodies at 1:100 dilution Nicotiana tabacum
mAb 11B1 does not detect CBP70 in plastids Zea mays
next step will be to localize minute amounts of secretions in intracellular compartments Meloidogyne incognita
(MORF8, RIP1, AT3G15000) /ICR1-specific polyclonal antibody recognizes 42-kD protein of expected molecular weight of unmodified (MORF8, RIP1, AT3G15000) (ICR1, RIP1, AT1G17140) Arabidopsis thaliana
(MORF8, RIP1, AT3G15000) /ICR1-specific polyclonal antibody recognizes 55-kD modified (MORF8, RIP1, AT3G15000) (ICR1, RIP1, AT1G17140) protein Arabidopsis thaliana
bound GST-fusions detected by immunoblotting using (ATGSTU24, GST, GSTU24, AT1G17170) antibodies
(ADL3, CF1, DL3, DRP2B, AT1G59610) β (chloroplast membrane ATPase β subunit) was detected by antibody to (ADL3, CF1, DL3, DRP2B, AT1G59610) β Chlamydomonas reinhardtii
crude extracts from leaves showed characteristic doublet bands at 39–42 kDa Glycine max
GmFAD7 protein distribution in developing seeds showed band of approximately 80 kDa Glycine max
2D chloroplast proteome immunoblotted with p FNR-specific antibody Triticum aestivum
SSIII identified as band j Zea mays
antibodies with different specificities showed differences in intensity of labelling Triticum aestivum
polyclonal antibody detected smaller cross-reacting bands Medicago sativa
mitochondrial fraction from Arabidopsis Col-0 leaves was tested by immunoblotting with antiserum specific for mitochondrial protein PDH and anti-glutathione antiserum Arabidopsis thaliana
antibodies with different specificities showed no differences in intensity of labelling Triticum aestivum
UVR8C antibody recognizes native (AtUVR8, UVR8, AT5G63860) Arabidopsis thaliana
(MORF8, RIP1, AT3G15000) (ICR1, RIP1, AT1G17140) antibody followed by horseradish peroxidase-conjugated anti-rabbit secondary antibody
Detection performed Western blot analysis
GmFAD7 antibody showed reaction in chloroplasts isolated from plant species Lotus japonicus; Medicago sativa; Phaseolus vulgaris; Vigna unguiculata; Arabidopsis thaliana
Plastidial photosystem II D1 protein ( (PSBA, ATCG00020) /D1) detected by western blotting Solanum lycopersicum
(FMO, AT1G12200) GS-OX1–YFP fusion protein was detected by western blot using anti-YFP antibody
confocal microscopy was used in indirect assay to confirm (ANY1, AtCESA1, CESA1, RSW1, AT4G32410) F954L protein was being expressed in experimental lines Arabidopsis thaliana
Detection was performed using SuperSignal West Dura Detection Kit (Thermo Fisher Scientific) Arabidopsis thaliana
BDR1-MYC is tagged protein
fractions of PINA and PINB proteins from seeds of T1 segregating progeny plants of 15B line were weak Triticum aestivum
antibodies generated against the phosphopeptide from human pRb protein cross-reacted with 115 kDa alfalfa protein Medicago sativa
antigenic distinctiveness of R. rubrum Rubisco can enable level of assembled and unassembled introduced Rubisco subunits to be examined rapidly using immunoblot analyses Nicotiana tabacum; Rhodobacter rubrum
immuno-reactive signals with MWs of 55 kDa and 35 kDa in wild-type Koshihikari are more intense than in transgenic with GluA2 line Oryza sativa
NAD-SDH is detected in crude protein Malus domestica
NAD-SDH is not detected in plasma membrane fraction Malus domestica
2D electrophoresis and immunoblot analysis detected 21 immunoreactive spots Helianthus annuus
T2 line 9-4-3 yielded very weakly visible protein bands of PINs Triticum aestivum
(GRL, LPL3, NAP1, NAPP, AT2G35110) antibody detects band at ~120 kDa Arabidopsis thaliana
root cells fixed and immuno-labeled with ATG8 antibody Arabidopsis thaliana
Western blotting is protein detection method
PVDF membrane was stained with Coomassie brilliant blue G-250
secondary antibodies incubated for 1 h at room temperature Cyanidioschyzon merolae
α-BES1 antibody is used at 1:1000 dilution
SSIIa detected by immunoblot analysis Zea mays
transgene product in mGluA2 transgenic rice seed is detected as band of 55 kDa Oryza sativa
IFRN 0610 monoclonal antibody does not recognize epitope in HMW glutenins or tagged LMW subunit encoded by transgene Triticum aestivum
NAD-SDH is detected in endomembrane fraction Malus domestica
Anti-GluD antibody reacted with both a4J and a4M Oryza sativa
antibodies against human caspase-3 active enzyme cross-reacted with DEVDase protein bands Dunaliella viridis
antibodies used by Corsi et al. (2004) and Lindemann et al. (2004) cross-reacted with various polypeptides from plant cell or antigen
cytosolic tubulin was immunodetected in both triton X-100- and digitonin-permeabilized GR2-GFP-transformed cells Nicotiana tabacum
Band C did not react with antisera Zea mays
GmFAD7-2 protein was recognizable by GmFAD7 antibody Glycine max
proteins isolated from chromoplasts reacted with anti (RBCL, ATCG00490) antibodies Solanum lycopersicum
cross-reactivity of the anti-Am (SUT1, AT5G63020) antibody might be responsible for labelling of sieve tubes in Knop et al. experiments
all protein bodies observed in sections prepared from transgenic seeds at 16 (DPA, AT5G02470) were labelled by A14 antibody Triticum aestivum
(ATMAPK3, ATMPK3, MPK3, AT3G45640) and (ATMAPK6, ATMPK6, MAPK6, MPK6, AT2G43790) of Arabidopsis can be detected by anti-pERK antibody in immunoblotting analyses Arabidopsis thaliana
expressed proteins were detected by immunoblotting Arabidopsis thaliana
null segregant seeds showed no labelling in sections prepared from Triticum aestivum
anti-MdSDH6 serum recognizes MdSDH6 Malus domestica
PUL activity band was also detected as blue band on native-PAGE gel containing potato amylopectin Oryza sativa
same fractions were reacted with anti-RPL10 antibodies Nicotiana benthamiana
hyperphosphorylated pRb protein is usually detected as more slowly migrating band Homo sapiens
(MORF8, RIP1, AT3G15000) /ICR1-specific polyclonal antibody recognizes 65-kD modified (MORF8, RIP1, AT3G15000) (ICR1, RIP1, AT1G17140) protein Arabidopsis thaliana
GmFAD7 protein was not detected in stems Glycine max
plasma membrane-bound peroxidases were identified by staining with guaiacol and H2O2 Zea mays
residual (AtPHOT2, NPL1, PHOT2, AT5G58140) from -1 allele is not readily detectable by Western blotting of total protein extracts Arabidopsis thaliana
UVR8N antibody shows no cross-reactivity with uvr8-1 mutant protein Arabidopsis thaliana
Anti-peptide antibody was used to test for HT-M protein expression in control and transgenic hybrids Nicotiana plumbaginifolia; Nicotiana alata
immunoblots were probed using antibodies against aleurain Arabidopsis thaliana
supernatant was analyzed by SDS–PAGE followed by protein blot Nicotiana tabacum
specific antibody raised against GmFAD7 protein reacted with single polypeptide band of approximately 39 kDa Glycine max
anti-ATG8 immunofluorescence used in Arabidopsis wild-type and (GRL, LPL3, NAP1, NAPP, AT2G35110) mutant Arabidopsis thaliana
Rabbit antiserum is used for specific detection of Arabidopsis AtPRF3 Arabidopsis thaliana
blots is developed with Pierce ECL/ECL Femto Western Blotting Substrate Arabidopsis thaliana
membrane is probed with anti-Myc-tag mouse monoclonal antibody (9B11)
immunoblotting is performed with antibodies diluted in blocking solution (5% fat-free milk in TBS with 0.1% Tween-20)
6×His-AtTSPO transgenic cell line showed immunodetection of (ATTSPO, TSPO, AT2G47770) Arabidopsis thaliana
A4 GAPDH tetramer has not been identified in all experiments
Arabidopsis thaliana contains two Rubisco activase (RCA, AT2G39730) cross-reacting bands Arabidopsis thaliana
immunoblot analysis detected six immunoreactive bands with molecular masses of 68, 59, 37, 30, 20, and 15 kDa Helianthus annuus
anti-Ntann12 polyclonal antibodies cross-react with polypeptide of ~54 kDa
chitinase antibody cross-reacted with protein from Bobwhite and all transgenic lines Triticum aestivum
no protein bands from the starch TAEP for both P310 and P311 was observed, while it was detectable by GM1 capture ELISA specific for LT-B detection Zea mays
Craspedostauros australis low molecular weight components can only be stained with silver stain Craspedostauros australis
αCaTrailin2 and -3 also recognized numerous small particles on substratum Craspedostauros australis
PROAtCAPE1 expression in three independent CAPE1ox CNYD transgenic lines examined by probing with anti-green fluorescent protein (GFP) antibody Arabidopsis thaliana
Rat monoclonal anti-HA-Peroxidase (clone 3F10) is detection reagent
primary antibodies are detected using secondary antibodies Arabidopsis thaliana
Anti-GST is detection reagent
approximately 66 kDa band detected by SSI-specific antiserum Zea mays
FK506-BINDING PROTEIN (FKBP) has yet to be detected through proteomics analyses
signal from immunoblot analysis was mainly from a band located at c. 25 kDa, consistent with the size of LHCI subunits Chlorella ohadii
immunoblotting analysis detected negligible amounts of additional phosphorylated subunits Chlorella ohadii
charophytes lack detectable putative phytochelatin synthase (PCS) bands by western blots
antibodies raised to the C-terminal 100 amino acids of (ATTIM17-2, TIM17, TIM17-2, AT2G37410) recognize 31-kDa protein from purified mitochondria Arabidopsis thaliana
antiserum raised to the C-terminus of Arabidopsis (ABCD1, ACN2, AtABCD1, CTS, PED3, PXA1, AT4G39850) recognizes both NBDs Arabidopsis thaliana
bands at the approximate size predicted for the fusion proteins (102 kDa) were detected in transgenic extracts Arabidopsis thaliana
(ATMAPK3, ATMPK3, MPK3, AT3G45640) band at ~43 kDa was weakly detectable in Y4 Arabidopsis thaliana
anti-phot2 antibody is highly specific for Arabidopsis (AtPHOT2, NPL1, PHOT2, AT5G58140) Arabidopsis thaliana
polyclonal antisera against central loop of FeSUT1 was confirmed for specificity by western blot analyses using plasma membrane proteins from FeSUT1-expressing yeast cells Saccharomyces cerevisiae
SSIIa-specific antiserum revealed band of approximately 75 kDa Zea mays
BTL homologue expression was characterized by immunolabelling with alkaline phosphatase Vitis vinifera
proteins in non-thermogenic plants are not detected by anti-AOX antibodies
anti-pERK antibody mainly detects short amino acid sequence containing phosphorylated Tyr 204 of ERK 1
anti-RNA polymerase II CTD repeat YSPTSPS antibody (8WG16) is detection reagent
PSTAIRE ( (CDC2, CDC2A, CDC2AAT, CDK2, CDKA1, CDKA;1, AT3G48750) p34) is detected by anti-cdc2 antibody
immunoblotting is performed with antibodies diluted in blocking solution Arabidopsis thaliana
α-BAK1 pS612 antibody is used at 1:3000 dilution Arabidopsis thaliana
DRM and DSM is probed using anti-FLS2 polyclonal antibody Arabidopsis thaliana
collected fractions were subjected to immunoblotting with anti-GFP antibodies Nicotiana benthamiana
anti-phot2 antibody does not recognize native protein from N. benthamiana Nicotiana benthamiana
anti-SSIIIa antibody recognized multiple bands Oryza sativa
total GFP is detected using GFP polyclonal antibody (Santa Cruze, B-2, 1:1000 dilution) Arabidopsis thaliana
cross-linked Lys-C fragment is immunoprecipitated by antibodies raised against the Ile206–Glu228 peptide Nicotiana tabacum
TBST buffer is used in western blotting
Xenopus Na + /K + ATPase α1 is detected using mouse anti-Na + /K + ATPase α1 antibody Xenopus laevis
SDS-PAGE and immunoblotting used (ATOST1, OST1, P44, SNRK2-6, SNRK2.6, SRK2E, AT4G33950) /42 MAPK (Erk1/2) antibody
Rabbit anti His-tag mAb is immunodetection reagent
Ponceau S is used for protein visualization
horseradish peroxidase-conjugated anti-mouse secondary antibodies (1:10,000 dilution; Millipore) was used for 1 h incubation Arabidopsis thaliana
peroxidase-conjugated goat anti-rabbit or mouse IgG is used at 5000-fold dilution
Tris-buffered saline (TBS) buffer is buffer solution
Western blotting detects specific proteins
Peach leaf sheath proteins were analyzed using anti-MP54 and anti-MP22 antibodies Prunus persica
OsNMD3-specific antibody indicated specificity of antibody Oryza sativa
clathrin antibody detected in plant extracts band of 190 kDa corresponding to predicted size of plant clathrin heavy chain Arabidopsis thaliana
monoclonal antibody against OsMAGO1 was used to analyze expression of (HAP1, MAGO, MEE63, AT1G02140) Oryza sativa
anti-Ntann12 antibodies cross-react with protein of ~36 kDa in roots tobacco
anti-AtRBR1 antibody detects 115 kDa protein band Medicago sativa
GR2-GFP was visible in triton X-100- and digitonin-permeabilized cells Nicotiana tabacum
chloroplast protein accumulation was examined by immunoblot analyses Nicotiana benthamiana
separated proteins were analyzed by immunoblotting with polyclonal antibodies against (ATSS4, SS4, SSIV, AT4G18240) Arabidopsis thaliana
specific antibodies confirmed that arabinogalactan protein epitopes as markers of AI cell specification Hieracium praealtum
anti-rabbit secondary antibodies binds anti-TrxB antibody
anti-β-tubulin antibody is primary antibody
immunoblot analysis used antibodies against cpSecY
PIF4-FLASH and PIF5-FLASH were detected at each of evening time points (ZT12 and ZT16) in either control or warm temperature during night-time (WN) conditions Arabidopsis thaliana
use of monoclonal antibodies detected only glycosidic epitope of arabinogalactan proteins (AGPs) Arabidopsis thaliana
anti-glutathione antibody in total extracts only detects few abundant proteins
antibody raised against recombinant (ATOBGC, ATOBGL, CPSAR1, EMB269, EMB3138, Obg A-2, OBGC, OBGL, AT5G18570) protein reacts with protein of about 85 kDa in whole plants Arabidopsis thaliana
Western blot is followed by immuno-detection using the ECL system (Amersham, Freiburg, Germany)
fluorescent probes facilitate detection of labeled proteome on protein gels by fluorescence scanning
total yeast proteins is analyzed by immunoblot analysis
Subsequent immunoblotting demonstrated that RpoA:HA was present in the solubilized membrane fraction
GFP fluorescence is detectable in leaves and fruits of weakly expressing transplastomic line Sl-pRC31 Solanum lycopersicum
Heterologous expression of all recombinant proteins verified by immunoblot analysis of total protein extracted using 1% (SDS, AT1G14750) Spodoptera frugiperda
~20 kD band in pET-28a-orf182-transformed E. coli was obviously visible in total protein from pET-28a-orf182-transformed E. coli Escherichia coli
bottom part of membrane was incubated with anti TIP1-1 antibody
Goat anti-mouse IgG-HRP secondary antibody is used for immunoblot detection
extracted recombinant (GRACE, NILR1, AT1G74360) Ecto-domain protein was verified by Western blotting using anti-His antibody Escherichia coli
purified anti-FeSUT1 antisera was used for all experiments Fraxinus excelsior
photosynthetic complexes that harbor plastid-encoded subunits examined by immunoblot analysis Zea mays
(BGLU23, LEB, PSR3.1, PYK10, AT3G09260) were detected in proteome profiling Arabidopsis thaliana
Western blot analysis uses anti-Rubisco polyclonal antibodies
immunobiochemical detection uses ECL detection system Chlamydomonas reinhardtii
possibility that the antibody reacts poorly with the other subunits cannot be excluded Chlorella ohadii
DNA aptamer sensors can be applied to monitor proteins
radiolabeled iOE23 is detected in thylakoid membranes after NaBr treatment Arabidopsis thaliana
Western blot is protein detection method
antibodies raised against CsCCD2 recognized single band corresponding to CsCCD2 Crocus sativus
antibody against Arabidopsis (RCA, AT2G39730) does not detect α-form (RCA, AT2G39730) polypeptide Zea mays
αFLS2-peptide antibody does not detect FLS2 in fls2∆ plants Arabidopsis thaliana
myrosinases (AtTGG1, BGLU38, TGG1, AT5G26000) and (BGLU37, TGG2, AT5G25980) are not detected by MS Arabidopsis thaliana
LOC_Os06g05260 lacks proteome support Oryza sativa
Flag-tagged H2A.1 is strongly detected in WT/Flag-H2A.1 and Atbmi1a-1/ (AtBMI1B, ATDRIP1, AtRING1B, DRIP1, AT1G06770) /Flag-H2A.1 extracts Arabidopsis thaliana
BDR2-MYC is tagged protein
Luminata Forte Western HRP Substrate is used for signal detection
(ATFLS2, FLS2, AT5G63580) is detected using rabbit polyclonal antisera Arabidopsis thaliana
PVDF membrane was imaged by blotting with anti-FLAG (HRP-conjugated) or anti-GFP (HRP-conjugated)
nitrocellulose membrane is laboratory material
pull-down proteins are determined by western blot with α-His or α-MBP antibodies Escherichia coli
separated proteins were transferred to PVDF
anti-GFP antibody is reagent
RpoA:HA was immunoprecipitated and analyzed by silver staining after SDS-PAGE
signal is captured using Syngene G:box storm imager
SPORK2 is detected using rabbit anti-SPORK2 antibody Xenopus laevis
proteins were transferred onto nitrocellulose membrane
Anti-mouse-HRP is detection reagent for mouse antibodies
anti-HA-HRP is detection reagent for HA-tagged proteins
anti-rabbit IgG (whole molecule)–HRP secondary antibody is used for western blotting Arabidopsis thaliana
7.3 kDa band was not recovered by mock immunoprecipitation without anti-Ile206–Glu228 antibodies Nicotiana tabacum
Immunohistochemical detection examined EMB12 protein expression in 7 days after pollination (DAP) embryo and endosperm Zea mays
membrane rafts are generally acknowledged to form below the resolution limit of the light microscope
anti-MPK4 antibody is specific for (ATMPK4, MAPK4, MPK4, AT4G01370) Arabidopsis thaliana
protein extracts are blotted onto membrane
Amersham Typhoon phosphorimager is used for autoradiography
proteins were detected using anti-GFP and anti-FLAG antibodies Nicotiana benthamiana
SEC12 antibodies are specific Arabidopsis thaliana
[125I]ASA-CLE9p specifically labels CLV1-HT Nicotiana tabacum
immunoprecipitation experiments showed that recombinant At CysPc–C2L–GFP fusion proteins harboring single mutations and double mutation E302Q/D309N were expressed in Arabidopsis thaliana Arabidopsis thaliana
(ICL, AT3G21720) (ICL1) protein was identified in iclC/WT comparison Chlamydomonas reinhardtii
isolated pea chloroplasts solubilized with 1% Triton X-100 and analyzed by 1D BN-PAGE and immunoblotting failed to detect consistent signals for (MAR1, TOC75, TOC75-III, AT3G46740) Pisum sativum
SP-mRFP-SUBEX-C57Y-EMP12 expression was hardly detectable by immunoblots in the different Arabidopsis lines Arabidopsis thaliana
ER-retained SP-mRFP-SUBEX-C57Y-EMP12-ER variant shows very low amounts of free mRFP Arabidopsis thaliana
confocal microscopy verified GFP expression of quadruple mutant fusion protein Arabidopsis thaliana
anti-PsRBR antibody immunoprecipitated higher and lower molecular mass forms of the phosphorylated PsRBR1 Pisum sativum
non-transgenic a123 shows no signal in anti-GluA antibody immuno-blotting analysis Oryza sativa
transgenic subunits are specifically recognized by commercial anti-c-myc antibodies Triticum turgidum subsp. durum
ten-day-old seedlings are used for GFP detection Arabidopsis thaliana
Total Arabidopsis (ATMAPK6, ATMPK6, MAPK6, MPK6, AT2G43790) is detected using anti-Arabidopsis (ATMAPK6, ATMPK6, MAPK6, MPK6, AT2G43790) antibody Arabidopsis thaliana
secondary antibody-probed membrane is visualized using luminol-based chemiluminescent substrate
Total protein extraction and immunoblotting was performed according to a method described previously Arabidopsis thaliana
Coomassie Brilliant Blue staining is protein detection method
Western blotting is used to detect protein abundance
western blot procedure was performed using anti-GFP antibody (Life technologies, A6455) Arabidopsis thaliana
Rabbit polyclonal herpes simplex virus VP16 antibody is detection reagent
UDP-glucose pyrophosphorylase is detected using Agrisera antibody Arabidopsis thaliana
separated proteins were blotted onto PVDF membrane
mutant (ANY1, AtCESA1, CESA1, RSW1, AT4G32410) protein with only one amino acid change compared with wild-type prohibits use of immunoblotting to confirm presence of (ANY1, AtCESA1, CESA1, RSW1, AT4G32410) F954L Arabidopsis thaliana
N103 antibody recognized activated SIMK in Arabidopsis protoplasts upon co-expression of HA-tagged SIMK and its upstream activator SIMKK Arabidopsis thaliana
BDR3-MYC is tagged protein
enhanced yellow fluorescent protein (EYFP) is protein tag
(NZZ, SPL, AT4G27330) mutant shows no (AGO9, AT5G21150) signal Arabidopsis thaliana
anti-GFP-HRP is detection reagent
Myc–PDX fusion proteins are detected by monoclonal α-myc antibody
(ATFKBP12, FKBP12, FKP12, AT5G64350) in total protein extracts from Arabidopsis Col-0, plants overexpressing CO (35S: CO), and the T-DNA null mutant co-10 was analyzed using immunoblots with αFK antibody Arabidopsis thaliana
Rx1 N. benthamiana transiently transformed with Dex::CP105 RB or Dex::CP106 AVR constructs leaves were brushed with Dex 2 days after agroinfiltration and at 0, 2 and 4 hpda total protein fraction was isolated and used for immunoblotting Nicotiana benthamiana
antibodies to SlDXS2-specific peptides are raised against SlDXS2-specific peptides Solanum lycopersicum
MBP-tagged proteins are detected by anti-MBP antibody
signal is detected using Pierce ECL Western Blotting Substrate Arabidopsis thaliana
Rabbit polyclonal antibodies against (ATBAK1, ATSERK3, BAK1, ELG, RKS10, SERK3, AT4G33430) is used at 1:5000 dilution Arabidopsis thaliana
α-BAK1 antibody is used at 1:5000 dilution
proteomic analysis did not identify PmgA and AqpZ proteins Synechocystis
endogenous 130-kD protein cross reacted with anti-YFP antibody Arabidopsis thaliana
69-kD band represents unrelated UGT or different protein Crocus sativus
(REIL1, AT4G31420) and (FZF, REIL2, STCH4, AT2G24500) proteins were not detectable in total protein preparations of reil1-1 reil2-1 mutant Arabidopsis thaliana
Western blot analysis uses anti-His antibody
chemiluminescence signal recorded on autoradiography film
immunoblots detected by film