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protein characterization

11962 relationships annotated with this phrase. Showing first 500 of 11962.
Source entity Relationship Target entity Species
MpCRY biochemical characteristics assessed by multiple protein-binding assays Marchantia polymorpha
type-I (PMES, AT4G10050) consist of signal peptide for secretion, 20 kDa inhibitory domain and 35 kDa PME domain
Nb (scpl25, AT3G02110) (NbL02g23560) peptograph displayed peptides at 40, 30 and 25 kDa Nicotiana benthamiana
nearly all RLK-derived peptides originate from ectodomains Nicotiana benthamiana
gel filtration chromatography revealed majority of hydroxylamine solubilized adhesive material exhibited molecular masses > 450 kDa Craspedostauros australis
Nb GMCO representing predicted full-length protein lacking signal peptide Nicotiana benthamiana
His-GFP-tagged C-terminal fragment of double-tagged Nb (EDA2, AT2G18080) at 60 kDa Nicotiana benthamiana
mature MmNec3 protein has mature molecular weight of 32.2 kDa Melianthus minor
four other GH3-family β-xylosidases with Fn3 domain show peptides from only catalytic domain migrating at 60–65 kDa Nicotiana benthamiana
CaTrailin4 was expected to elute in high-molecular-mass fractions Craspedostauros australis
InterPro-predicted Pfam domains highlighted in peptograph
putative Golgi-localized peptide-N4-(N-acetyl-beta-glucosaminyl) asparagine amidase A (Nb PNGaseA, NbL17g03090) shows peptides of majority of protein at 58 kDa Nicotiana benthamiana
37 putative RLKs including likely orthologs of auxin co-receptor (TMK1, AT1G66150)
quantitative proteomics identified 676 N-glycosylation sites in 351 glycoproteins Colletotrichum graminicola
Most proteins (74.3%) not detected in single gel slice Nicotiana benthamiana
25 detected GELPs only show peptides at predicted molecular weight from entire catalytic domain Nicotiana benthamiana
60 kDa proteoform of Nb BXYL is C-terminally truncated Nicotiana benthamiana
putative α-mannosidase Nb AMAN (NbL03g02970, family GH38) shows peptides across protein at 130 kDa Nicotiana benthamiana
tagged N- and C-terminal fragments of double-tagged (scpl25, AT3G02110) accumulating at 65 and 55 kDa Nicotiana benthamiana
plant ML domain proteins have not been characterized at biochemical, cell biological, or functional level Arabidopsis thaliana
GhPME2 has isoelectric point of 9.23 Gossypium hirsutum
size of protein detected by antibody corresponds well to size of recombinant protein Arabidopsis thaliana
17 SAM domain-containing proteins in barley have not been functionally characterized except for EGT2 Hordeum vulgare
four other detected BGALs of family GH35 have similar peptographs Nb (BGAL1, AT3G13750) Nicotiana benthamiana
gel slice at 60 kDa lacks peptides of FN3 domain Nicotiana benthamiana
work on (ALMT9, AtALMT9, AT3G18440) provides new molecular, biochemical, and biophysical details about transmembrane domain (TMD) of ALMT (aluminum-activated malate transporter) family member Arabidopsis thaliana
59 proteins caused by protein insolubility or protein being too large to migrate into polyacrylamide gel Nicotiana benthamiana
five additional SCPLs show peptides mostly at predicted molecular weight Nicotiana benthamiana
(RLK, AT5G67280) peptides in PROTOMAP dataset unexpected because sample preparation included removal of insoluble material by centrifugation before boiling in lithium dodecyl sulfate Nicotiana benthamiana
Nb PME51 (NbL05g19120) detected at 60 kDa Nicotiana benthamiana
PROTOMAP dataset contains peptides of receptor-like kinases (RLKs) Nicotiana benthamiana
hA1AT and purified Strep A1AT incubation with phosphate-buffered saline (PBS) did not alter protein sizes detected by western-blot analysis
soluble fraction of chloroplast proteins contains same pattern of acidic and basic AtFNR forms Arabidopsis thaliana
isolated membranes treated with protein phosphatase inhibitors showed unchanged pattern of FNRs on 2D gels Arabidopsis thaliana
FNR-containing protein complexes were analyzed by LC-MS/MS Arabidopsis thaliana
membrane-bound and soluble proteins were detected by immunolabeling with FNR antibody Arabidopsis thaliana
mucin-like protein in salivary proteome of BPH has been detected BPH salivary proteome Nilaparvata lugens
A4-GAPDH/ (CP12, CP12-2, AT3G62410) (PRK, AT1G32060) complex has molecular weight of 500 kDa Arabidopsis thaliana
peptides from prodomain absent in any gel slice Nicotiana benthamiana
PROTOMAP dataset contains 33 GDSL esterases/lipases (GELPs) Nicotiana benthamiana
putative β-arabinofuranosidase Nb BARA (NbL17g06930, family GH127) detected at 88 kDa Nicotiana benthamiana
truncation of RLKs consistently 50–70 kDa Nicotiana benthamiana
25 detected GELPs indicating not processed Nicotiana benthamiana
C-terminal region of bacterial GH127 enzyme has homology to two β-sandwich domains
purified leaf ZmRCAβ has determined molecular mass of 43.3 kD Zea mays
Nb HEPA also has peptides of catalytic domain at 35 kDa Nicotiana benthamiana
Nb (EDA2, AT2G18080) and C-terminal peptides at 25 kDa Nicotiana benthamiana
heparanase-like Nb HEPA (NbL13g02440, family GH79) has peptides covering most of protein at 58 kDa Nicotiana benthamiana
catalytic Asp and His residues of Nb (EDA2, AT2G18080) at C terminus Nicotiana benthamiana
37 putative RLKs including likely orthologs of (ATRALF1, RALF1, RALFL1, AT1G02900) receptor FERONIA
putative CitCCD4 protein had an estimated molecular mass of 63 kD
His-GFP-tagged C-terminal fragment of double-tagged Nb PNGaseA consistent with peptograph Nicotiana benthamiana
efficient reconstitution was confirmed by flotation experiments
CaTrailin4 PTS repeats constitute approximately 70% of sequence Craspedostauros australis
TGB3 protein has been difficult to study due to low abundance in comparison to TGB1 and TGB2 movement proteins
59.7% of 5,040 detected proteins detected as multiple proteoforms Nicotiana benthamiana
tripeptidyl peptidase (NbTPP2, NbL03g11790) identified at 150 kDa Nicotiana benthamiana
Nb PME51 (NbL05g19120) accumulates as uncleaved precursor Nicotiana benthamiana
37 putative RLKs including likely orthologs of chitin receptor (AtCERK1, AtLYK1, CERK1, LYK1, LYSM RLK1, AT3G21630)
uncleaved SCPLs have short linker region (4–15 residues)
four unprocessed (PMES, AT4G10050) lack basic residues in cleavage region Nicotiana benthamiana
3,011 proteins indicating multiple proteoforms Nicotiana benthamiana
PROTOMAP dataset contains peptographs of 18 SCPL proteins Nicotiana benthamiana
cleaved SCPLs have highly polymorphic linker sequences
Nb PME51 (NbL05g19120) close to predicted molecular weight of 62 kDa Nicotiana benthamiana
C-terminal region of Nb BARA detected at 15 kDa Nicotiana benthamiana
Pro-Q Diamond phosphoprotein gel stain provided negative results Arabidopsis thaliana
citrate lyase large subunit (CLS) has molar absorption coefficient of 50 545 M−1 cm−1 Chlorobaculum tepidum
estimated molecular mass of monomer is overestimation
transmembrane domains was predicted using bioinformatics methods
protein homologies was predicted using bioinformatics methods
PROTOMAP dataset contains 12 type-I pectinemethylesterases (PMEs) Nicotiana benthamiana
AtFNR1 contains N-terminal peptides starting with AQVTTDTT, QVTTDTT, and VTTDTT Arabidopsis thaliana
eluted fractions were analyzed by 15% SDS–PAGE
few peptides from kinase domains despite being substantial part of (RLK, AT5G67280) protein Nicotiana benthamiana
corresponding enzymes have been characterized Fragaria x ananassa
ZmRCAα protein corresponds to α-form (RCA, AT2G39730) reported in other species Zea mays
βC-plastoglobuli seem to contain major larger protein of about 32 kD Dunaliella bardawil
major band of 53 kD corresponds to CsALDH3I1 protein Crocus sativus
purified GhPMEI3 protein has molecular weight of 43.96 kD Gossypium hirsutum
150-k fraction and soluble cytosolic fraction are subjected to ESI–LC–MS analysis Synechocystis 6803
different glutaredoxin (GRX) isoforms may exhibit different biochemical properties
sedimentation analysis was performed using analytical ultracentrifuge XL1
approximately 30-kilodalton band is slightly larger than GFP used as control
acidic AtFNR spots show unsuccessful sequencing indicating possible N-terminal blocking Arabidopsis thaliana
biochemical approaches described mostly unidentified DNA binding proteins in plastids
size of protein detected by antibody is larger than expected size from amino acid sequence of mature (ATOBGC, ATOBGL, CPSAR1, EMB269, EMB3138, Obg A-2, OBGC, OBGL, AT5G18570) Arabidopsis thaliana
dual targeted proteins from Arabidopsis thaliana were selected for sequence analysis Arabidopsis thaliana
soluble FNR pool shows low protein abundance Arabidopsis thaliana
YFP-GS has expected molecular weight of 80 kD Arabidopsis thaliana
CsUGT74AD1 was found in insoluble fraction Crocus sativus
supernatant fraction contains several proteins Synechocystis 6803
first peptide found corresponded to amino acids 11-25 (TDTSHHDQDHPTFNK)
A1AT incubation with IF previously heated to 95°C resulted in detection of only full-length A1AT
mature structure of bioactive CLE peptides has only been determined for a limited number of plant CLEs
ThrRS–dTP(2–60) peptide was digested with trypsin Escherichia coli
Coomassie Brilliant Blue staining showed absence of 52-kilodalton band
purified recombinant OsNTRC was analyzed in absence of DTT Oryza sativa
several truncated forms of RCL-containing peptide could be found in peptide mapping
immunoblot analysis using anti-phospho-Thr, anti-phospho-Ser, or anti-phospho-Tyr antibody provided negative results Arabidopsis thaliana
(ALY1, AT5G59950) and truncated versions of the protein were examined by SDS-PAGE
(ATXYL1, AXY3, GH31, TRG1, XYL1, AT1G68560) α-xylosidase has apparent molecular masses of between 75 and 100 kDa Arabidopsis thaliana
recombinant Arabidopsis FC lyase migrates at apparent molecular mass of 67 kDa Arabidopsis thaliana
VvMYC1 is first grapevine (bHLH, AT5G51780) protein characterized Vitis vinifera
endogenous (AS2, AT1G65620) protein has molecular mass of 25 kDa Arabidopsis thaliana
OsPIP1;3 from a drought-resistant rice variety characterized by biochemical and biophysical approaches Oryza sativa
linker region between N- and C-terminal domains exhibited flexibility in sequences and length Strobilanthes cusia
both proteins were detected as full-length versions after incubation with IF at pH 7.0
similarly truncated form of (ATSS4, SS4, SSIV, AT4G18240) recombinantly expressed in E. coli was measurable by zymogram Escherichia coli
size of protein detected by antibody corresponds well to size of mAtOBGL observed in import assays Arabidopsis thaliana
peptides from conserved N-glycosylation site lack expected +3-D mass shift at Asn Arabidopsis thaliana
glycoproteins have been identified in Arabidopsis chloroplasts Arabidopsis thaliana
40-kilodalton form of A1AT accounts for about 60% of A1AT expression level
JIM13 antibody detects arabinogalactan protein-related glycan
these proteins can run at higher molecular mass Arabidopsis thaliana
desired protein fractions were confirmed by SDS-PAGE
fraction enriched in (GASA14, AT5G14920) staining with lectin specific for galactose was positive Arabidopsis thaliana
SS4-YFP has expected molecular weight of 141 kD Arabidopsis thaliana
tracing protein thermal stability by recording changes in (TRP, AT3G56390) residues in increasing temperature gradient
SfLPAT protein has predicted mass of 43,723 D Steculia foetida
(ATPME2, PME2, AT1G53830) (PMES, AT4G10050) have apparent molecular mass of approximately 35 kDa Arabidopsis thaliana
(GASA14, AT5G14920) protein staining with Yariv reagent was negative Arabidopsis thaliana
purified leaf ZmRCAα has determined molecular mass of 46.1 kD Zea mays
52-kilodalton band corresponded to full-length protein
basic AtFNR2 spot has N-terminal sequence QITTE Arabidopsis thaliana
YFP-SS4N-GS has expected molecular weight of 137 kD Arabidopsis thaliana
YFP-SS4C activity could not readily be detected using zymograms
up to ∼100 transcription factors might be imported into plastids are not described at biochemical level
low-molecular-mass form of (NTRC, AT2G41680) has molecular weight of 153 ± 13 kDa
recombinant His-tag ZmRCAα has larger molecular mass than recombinant His-tag ZmRCAβ Zea mays
western-blot analysis of TSP extracted from transformed plants showed two signals: one at approximately 52 kilodaltons and other at approximately 40 kilodaltons Arabidopsis thaliana
Arabidopsis ALY proteins were systematically studied for expression and subcellular localization Arabidopsis thaliana
shape of the melting curve allowed conclusion that protein is properly folded
phosphorylated GST-fused SOS2CA analyzed by mass spectrometry
CsUGT74AD1-thioredoxin fusion protein has molecular mass of 69.7 kD Escherichia coli
(HGL1, AT3G23640) is expected to have higher molecular mass Arabidopsis thaliana
nanoDSF technology relies on tracing protein thermal stability by recording changes in (TRP, AT3G56390) residues
malate dehydrogenase (MDH, pNAD-MDH, AT3G47520) has molar absorption coefficient of 14 565 M−1 cm−1 Chlorobaculum tepidum
55-kDa protein is larger than predicted 41 kDa Hordeum vulgare
protein functional domains was predicted using bioinformatics methods
all studied complexes contained (AtTic62, Tic62, AT3G18890) and (TROL, AT4G01050) Arabidopsis thaliana
GhPMEI3 has theoretical molecular weight of 25.96 kD Gossypium hirsutum
Two-dimensional gel analysis of leaf extracts allowed the identification of basic and acidic isoforms of 2-Cys Prx A and B Arabidopsis thaliana
analysis of glutaredoxin (GRX) function is a difficult task because of the presence of approximately 30 different glutaredoxin (GRX) isoforms in plants
DTT treatment is confirmed by SDS–PAGE and western blot analysis
steady-state fluorescence measurements performed on purified recombinant proteins
AtFNR2 contains N-terminal peptides starting with AQITTETD, QITTETD, and ITTETD Arabidopsis thaliana
Ca5609 PTS repeats constitute approximately 50% of sequence Craspedostauros australis
CER-ZA protein was characterized after expression in yeast Hordeum vulgare; Saccharomyces cerevisiae
truncation of RLKs irrespective of type of (RLK, AT5G67280) Nicotiana benthamiana
phosphatase treatment of immunoprecipitated AtFNR using Phos-tag acrylamide showed no shift in electrophoretic mobility of AtFNR Arabidopsis thaliana
mature (ATFD1, FD1, AT1G10960) and (ATFD2, FD2, FED A, AT1G60950) were purified and covalently bound to Sepharose resin Arabidopsis thaliana
salivary gland mucins have been identified in insects
Trx-1 protein has molar absorption coefficient of 18115 M−1 cm−1 Chlorobium tepidum
partial N-terminal acetylation of the ε-subunit of ATP synthase resulted in practically identical migration pattern on the 2D gels as compared with that of AtFNR Citrullus lanatus; Arabidopsis thaliana
proteomic and genetic research resulted in identification of novel nuclear-encoded subunits higher plants
evidence for various ferritin subunits has been reported in literature for different plant species including Arabidopsis Arabidopsis thaliana
Arabidopsis 4-CL-like CoA ligases were purified as soluble enzymes Arabidopsis thaliana
other biochemical approaches should be considered full revelation of structural features of recalcitrant protein Arabidopsis thaliana
recombinant Arabidopsis FC lyase migrates by SDS-PAGE at apparent molecular mass approximately 11.7 kDa greater than predicted molecular mass Arabidopsis thaliana
GS protein sequence length in Arabidopsis ranges from 353 aa (GLN1;5) to 430 aa (ATGSL1, GLN2, GS2, AT5G35630) Arabidopsis thaliana
molecular basis for TRXz specificity towards its targets remains to be deciphered
(DMT7, DRM2, AT5G14620) undergoes structural and functional characterization
protein band of ~80 kDa is consistent with expected size of CSLC protein Hordeum vulgare
bioinformatic tool developed to locate N-terminus of mature secreted proteins Arabidopsis thaliana
PCB eluted in 26.5 mL fraction contrasts with AtCRL∆TM-PCB complex and AtCRL∆TM alone eluted in 19 mL fraction
(ATFH8, FH8, FORMIN 8, AT1G70140) (FH1FH2) sedimented at 58 and 117 kDa during sedimentation-velocity analytical ultracentrifugation
UGT proteins showed diverse N-terminal residues that mainly interact with diverse acceptor and conserved C-terminal residues that principally interact with specific sugar donor Strobilanthes cusia
6970 molar extinction coefficient was calculated by PROTPARAM program
VGT can be characterized using isolated vacuoles
His-tagged PipCoA ligase has calculated isoelectric point of 8.03 Piper nigrum
each spot identified for molecular weight and isoelectric point after calibration to commercially available protein standards
CD measurements carried out in Jasco 720 spectropolarimeter
incubated mixture subjected to ATPase activity measurements
water and nitrate permeability of OsPIP1;3 systematically characterized Oryza sativa
FcpA His complex showed slight blue shift of 1.5 nm Phaeodactylum tricornutum
most S-RNases and relic S-RNases are basic proteins with only one intron
predicted polypeptide of 335 amino acid residues has isolectric point of 9.6
two individual strategies confirmed that (ATFH8, FH8, FORMIN 8, AT1G70140) (FH1FH2) was able to form dimers
RNA-binding proteins (RBPs) have not been characterized experimentally experimental characterization Arabidopsis thaliana
overexpression of MST(-like) transporters have enabled functional characterization of transporters
(AAT2, ASP2, AT5G19550) suppressor proteins are identical to each other regarding lack of both a covalently bound (ATFTA, FTA, PFT/PGGT-IALPHA, PLP, AT3G59380) cofactor and enzymatic activity
FPLC (Fast Protein Liquid Chromatography) in combination with SDS–PAGE (SDS–polyacrylamide gel electrophoresis) were conducted to verify oligomeric state of (ATFH8, FH8, FORMIN 8, AT1G70140) (FH1FH2)
stabilizing and destabilizing mutants of titin were previously analyzed by atomic force microscopy
(PG45, PGA4, AT1G02790) expressed in Escherichia coli was used for subcellular localization and PG activity analysis Arabidopsis thaliana; Escherichia coli
His-tagged PipCoA ligase monomer has calculated mass of 61.2 kDa Piper nigrum
AtCRL∆TM-PCB complex displayed increased absorption value of ≈ 1100 mAU
apparent molecular weight of CrTRXz is 30 ± 5 kDa Chlamydomonas reinhardtii
five of these FARs have been biochemically characterized biochemical characterization Arabidopsis thaliana
weak signal from glycoprotein staining appears to be unspecific Arabidopsis thaliana
93 (PSS1, AT3G59640) homologs have not been characterized
proteins in three thylakoid fractions and cytosol are resolved using SDS–PAGE Synechocystis 6803
genetic analyses will also have to be performed to further define importance of these enzymes in vivo
sedimentation analysis of (NTRC, AT2G41680) in presence of DTT identified complex at 5.5 S
seed protein profile analyzed by one-dimensional electrophoresis (1-DE) Medicago truncatula
HMW (670–440 kDa) and LMW (250–140 kDa) fractions from GPC were subjected to non-denaturing SDS-PAGE Zea mays
N-terminal domain has high content of hydroxylated residues (Ser, Thr) Glycine max
minor negative bands at (–)612 nm and (–)615 nm corresponded to Q x absorbance of Chl a or Q Y absorbance of Chl c Phaeodactylum tricornutum
S-RNases of the genus Prunus have two introns Prunus
SSI, SSIIa and SBEIIb in LMW fractions were seen to migrate much further gel compared with HMW fractions Zea mays
peptide mass spectrometry confirmed homopropargylglycine (HPG) incorporation sites Arabidopsis thaliana
relative electrophoretic mobility of SSI, SSIIa and SBEIIb following GPC was determined following GPC fractionation Zea mays
multiple polypeptides of relatively small molecular mass, approximately 25–50 kDa were identified directly by amino acid sequence as fragments of GBSS
purified proteins demonstrated slower electrophoretic mobility than calculated molecular weights
(AtKAT1, KAT1, AT5G46240) is chosen as prototype for systematic investigation of structural and functional distinctions Arabidopsis thaliana
PipCoA ligase has calculated isoelectric point of 7.83 Piper nigrum
crude proteins extracted from rosette leaves of WT and (AAO3, AOdelta, At-AO3, AtAAO3, AT2G27150) mutants, as well as WT, (AAO1, AO1, AOalpha, AT-AO1, ATAO, AtAO1, AT5G20960) mutant and A1OE leaves were fractionated on native gel Arabidopsis thaliana
mature protein sequence of AtPRIN2 confers acidic pI of 4.43 Arabidopsis thaliana
aldehyde oxidase 1 (AAO1, AO1, AOalpha, AT-AO1, ATAO, AtAO1, AT5G20960) has similar mobility in native polyacrylamide gel electrophoresis Arabidopsis thaliana
molecular weight of GS protein in Arabidopsis varied between 38.59 kDa (GLN1;3) and 47.41 kDa (ATGSL1, GLN2, GS2, AT5G35630) Arabidopsis thaliana
SrUCPA and SrUCPB proteins were analysed by SDS-PAGE and immunoblotting Symplocarpus racenosus
fractions at 13.5 and 18.8 ml from gel filtration contained Trx-o protein Pisum sativum
PsPrxIIF shows very high hydrophobicity
hypocotyl extracts analyzed by LC-MS/MS method
protein identified here showed 92% identity to proteins described by Rodrígues-López et al. (2001) Triticum aestivum
Agrobacterium tumefaciens-mediated transient expression (agroinfiltration) is used throughout plant science for biochemical characterisation of proteins
Guinier analysis radii of gyration of CrTRXz allowed computation of molecular weight intervals of 15.80–17.75 kDa and 18.35–20.85 kDa at 95% credibility Chlamydomonas reinhardtii
combined ATP-affinity screening with reverse-genetic analyses show great potential for identification of ATP targets and protein assignments to previously unknown physiological functions Arabidopsis thaliana
functional characterization of putative HCN transporters in cassava is required Manihot esculenta
agromonas assay is used to perform structure-function analysis Nicotiana benthamiana
PEROXIDASE 52 (PRX52, AT5G05340) has mature protein of approximately 34.2 kDa Arabidopsis thaliana
117 kDa sedimentation value represented dimers of (ATFH8, FH8, FORMIN 8, AT1G70140) (FH1FH2)
presence or absence of β-MeOH or DTT in isolated chloroplasts did not have any effect on protein distribution profile Glycine max
beta-xylosidase 1 (PhXYL1) was protein isoform Petunia×hybrida
tomato GAGT displays very high substrate specificity tomato
subtilisin in soybean xylem fluid and Medicago truncatula suspension culture fluid is at approximately 80 kDa molecular weight Medicago truncatula; Glycine max
YFP has molecular weight of 27 kDa
MS analysis of CrTRXz showed only monomeric species under native or denaturing conditions Chlamydomonas reinhardtii
Arabidopsis (CMT3, AT1G69770) /maize ZMET2 undergoes structural and functional characterization
All GS proteins have an isoelectric point (pI) close to acidic pH (5.12–6.43 > pH 7)
isozymes GLN1;5 in Arabidopsis and OsGS1;3 in rice has highest solubility compared with other GS isozymes Arabidopsis thaliana; Oryza sativa
photo-affinity labelling was employed as alternative technique to confirm ATP-binding by SUBTILISIN-LIKE SERINE PROTEASE 1.7 (ARA12, SBT1.7, AT5G67360) Arabidopsis thaliana
HCC1-SNAP with molecular weight of approximately 51 kDa could represent either 48 kDa or 56 kDa version of the SNAP-tagged (HCC1, AT3G08950) Arabidopsis thaliana
(AAO1, AO1, AOalpha, AT-AO1, ATAO, AtAO1, AT5G20960) knockout mutant was employed because aldehyde oxidase 1 (AAO1, AO1, AOalpha, AT-AO1, ATAO, AtAO1, AT5G20960) has similar in-gel mobility to aldehyde oxidase 3 (AAO3, AOdelta, At-AO3, AtAAO3, AT2G27150) protein Arabidopsis thaliana
GS protein sequence length in rice ranges between 356 aa (OsGS1.1) and 428 aa (OsGLN2) Oryza sativa
coverage assigned using MASCOT
(AtETR1, EIN1, ETR, ETR1, RDO3, AT1G66340) 165–738 has two minima at 210 and 219 nm in CD spectra
GSE1 protein in soluble fraction was assessed by SDS-PAGE Escherichia coli
protein of 453 amino acids (first cDNA) has pI of 8.45 Glycine max
presence of excess of iron (Fe 2+ ) made relative distribution of GmFAD7 protein conformations similar to control extracts Glycine max
32 kDa polypeptide is consistent with molecular weight of mature (XTH1, XTR22, AT4G13080) Cicer arietinum
correct folding and structure of purified recombinant ETHYLENE RECEPTOR 1 (AtETR1, EIN1, ETR, ETR1, RDO3, AT1G66340) proteins were confirmed by circular dichroism Arabidopsis thaliana
differences between apparent and theoretical molecular mass are related to alterations in detergent binding in SDS-PAGE Glycine max
prominent single negative band at (–)670 nm was observed in WT Fcps Phaeodactylum tricornutum
colorimetric method is used for biochemical characterization of plant (ATSRX, SRX, AT1G31170) Arabidopsis thaliana
tobacco leaf proteomics has been used to study apoplast proteome
band f identified as BEIIb Zea mays
(TOD1, AT5G46220) orthologs from both angiosperms and gymnosperms have been further characterized characterization of (TOD1, AT5G46220) orthologs
four basic POX isozymes (B1, B2, B3, B4) were identified with pI values of 9.0, 8.4, 7.5, and 7.4 Brassica oleracea
apoplastic peroxidases can be more tightly bound to cell wall (extractable only with high-salt buffer)
huge split signal at (–)467 nm and (+)440 nm was observed in FcpA His Phaeodactylum tricornutum
Egl-27 and MTA1 have been cloned in Caenorhabditis elegans Caenorhabditis elegans
apparent molecular mass of GmFAD7 proteins (39–42 kDa) was different from theoretical mass deducted from amino acid sequence Glycine max
FcpA His protein shows broad but minor peak between 24–27 ml Phaeodactylum tricornutum
TaCAD1 protein has theoretical isoelectric point of 5.926 Triticum aestivum
His-mBnHO1 recombinant protein exhibited apparent molecular mass of 32.7 kDa Escherichia coli
recombinant (ATKTI1, AtKTI4, KTI1, AT1G73260) proteins protein concentrations were determined as in Bradford assay Escherichia coli
effect of in vitro changes of redox conditions from thiol groups was analysed on distribution of GmFAD7 proteins Glycine max
sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) was used for Western blot analysis Triticum aestivum
phloem protein 2 (PP2)-dimer has molecular weight of 48 kDa Cucurbita maxima
molecular weight of the fluorescent band was estimated to be higher than 180kDa Nicotiana benthamiana
Lepetit et al. (2007) could not find differences in CD spectra of Fcps in different oligomeric states Phaeodactylum tricornutum
ω/α-hpRNA lines 28A, D793, E140, and D874 show decreases in number and staining intensity of protein bands between 30 kDa and 70 kDa Triticum aestivum
BEIIa peak activities were found in fractions 6–13 Oryza sativa
SDS-PAGE confirmed purity and homogeneity of recombinant (AtETR1, EIN1, ETR, ETR1, RDO3, AT1G66340) and 165–738
peak showing 3 nm blue shift might suggest existence of heterogeneous pool of Chl a Phaeodactylum tricornutum
recombinant (ATKTI1, AtKTI4, KTI1, AT1G73260) proteins with six His residues at N-terminus size was determined by 12% SDS-PAGE Escherichia coli
purified HIS–AtSNX2b protein separated by SDS–PAGE
FcpA His protein has predicted molecular mass of 18.9 kDa Phaeodactylum tricornutum
absorption maxima at Q Y region of Chl a were consistently found at 672 nm Phaeodactylum tricornutum
prominent single negative band at (–)669 nm was observed in FcpA His Phaeodactylum tricornutum
genetically encoded protease activity reporters is collection of reporters using fluorescence, bioluminescence, or transcription as readout
enzymatic properties of recombinant L. perenne 6-SFT were examined and compared with properties of recombinant barley 6-SFT Lolium perenne; Hordeum vulgare
finding that SuSy2 is the second most abundant isoform further supports hypothesis that the de novo peptide sequence belongs to SuSy2 Medicago truncatula
WT Fcp fraction was also eluted from gel filtration column at 16.6 ml Phaeodactylum tricornutum
band at 38 kDa in C59S variant corresponded to faster mobility of the wild type Pisum sativum
non-reducing conditions in gel filtration showed two additional peaks at 12.9 ml and 16.6 ml Pisum sativum
acidic subunit of GluA2 transgenic rice migrates at same position as endogenous GluA2 gene product from non-transgenic Koshihikari Oryza sativa
pure His-tagged FcpA complex was characterized by purification and biochemical, as well as spectroscopic, characterization Phaeodactylum tricornutum
BN-PAGE analysis revealed heterogeneity in oligomeric forms of (PRK, AT1G32060) and plastid GAPDH
two peaks at 13.5 and 15.3 ml from gel filtration contained PsPrxIIF protein Pisum sativum
third ABA receptor has unclear molecular identity
immunoprecipitated samples subjected to western blotting Arabidopsis thaliana
detection of 39 kDa band was not sensitive to thiol redox conditions Glycine max
FcpC, D, and E have strongest signals in mass spectrometry Phaeodactylum tricornutum
shoulder at 486 nm is present in WT Fcp Phaeodactylum tricornutum
leaf peroxisomal proteins had a slightly acidic to strongly alkaline isoelectric point (IEP) Spinacia oleracea L.
predicted polypeptide of 335 amino acid residues has calculated molecular mass of 37.9 kDa
size of mature proteins is very close to that of cytosolic proteins
four spots with kDa/pI values similar to reported regulators of complementary activation proteins have comparable molecular weights but different pI values
BEIIb stains with Pro-Q Diamond
TRXf has been systematically compared to other TRX types
experimental structures at atomic resolution would ideally provide confirmation of thioredoxin complementarities
ABPP provides capacity to test activity and functionally annotate proteins
Arabidopsis AAP family members have been extensively characterized by heterologous expression in Xenopus oocytes Arabidopsis thaliana; Xenopus laevis
free eGFP has average lifetime of T D GFP=2.43 ns Arabidopsis thaliana
various growth temperatures, isopropyl β-d-1-thiogalactopyranoside concentrations, or adjustments of osmolytes resulted in no enzyme activity detected in soluble or insoluble fractions Escherichia coli
SDS-PAGE analysis on 12.5% acrylamide gel identified single band with calculated molecular size of 65 kDa Escherichia coli
two-dimensional gel electrophoresis (2DE) used to distinguish between four wheat p FNR protein isoforms Triticum aestivum
NrChit1 has molecular mass of 31.1 kDa Nepenthes rafflesiana
pmPOX2a monomer has experimental isoelectric point and relative molecular mass determined for the first time
BnHO1 gene encodes protein with predicted molecular mass of 32.6 kDa Brassica napus
conserved α-L-arabinofuranosidase motif at C-terminus of MdAF1 and MdAF2 is characteristic of GH51 family proteins
analytical ultracentrifugation might prove to be useful tool
mass difference in peptide SIGDGVQFLNR corresponds to replacement of D instead of N residue Medicago truncatula
peptides derived from (ASUS1, atsus1, SUS1, SUSY1, AT5G20830) and SuSy2 were detected during analysis of 1D-PAGE samples Medicago truncatula
SSI apparent molecular weight matches predicted value of the cDNA sequences Zea mays
in gel activity stainings detected peroxidases of a wide range of molecular weight Vigna unguiculata
glutelin fraction extracted with lactic buffer was subsequently subjected to gel filtration analysis Oryza sativa
OsCAS–YFP overexpression lines were generated to facilitate studies on enzyme kinetics, structural properties, and subcellular localization of OsCAS Arabidopsis thaliana
NO2-Tyr concentration in hypocotyls is very similar to NO2-Tyr concentration in mice liver
heterologously expressed UGT73F2 provided evidence demonstrating biochemical function and substrate specificity
two SSIIa mobility forms were previously suggested by analyses of starch granules in rice
mature OsCAS protein has molecular mass of 36.5 kDa Arabidopsis thaliana
one of the proteins described by Rodrígues-López et al. (2001) was found to be soluble
all Fcps in WT sample were also trimeric under particular condition of solubilization of thylakoids Phaeodactylum tricornutum
additional gel filtration in FcpA His was helpful to confirm that complex was extremely stable and functional Phaeodactylum tricornutum
pmPOX3 has native molecular mass of 40 kDa
xyloglucan-specific endoglucanase inhibitor protein (XEGIP) has well characterized expression and biological activity Solanum lycopersicum
120kDa and 60kDa APases partially purified from senescing leaves exhibit kinetic and immunological properties indicating purple acid phosphatases (PAPs) Hakea prostrata
SSI activity was found in addition to monomeric size (fractions 10–13) Oryza sativa
BE isozyme activities were visualized by zymogram analysis in the presence of G1P as a substrate Oryza sativa
SSI, BEIIb, and (ATPHO1, PHO1, AT3G23430) co-migrated in addition to respective monomeric protein sizes Oryza sativa
processed protein of Trx-o has p I of 6.3 Pisum sativum
saline-soluble glutelins were subjected to centrifugation on SDG Oryza sativa
Transformants producing re-introduced form I tobacco Rubisco variants were identified by non-denaturing PAGE Nicotiana tabacum
band g identified as BEI Zea mays
two electrophoretic mobility forms of SSIIa were absent from su2 mutant
targeted CKX enzymes have been studied so far research focus
relatively high abundance of SuSy in root nodules makes understandable that the conserved Ser11 phosphopeptide has been described several times Medicago truncatula
migratory distance between the two forms is small
Clp, FtsH, Deg, and CND41 are best characterized plastid proteases
three peptides with amino acid substitutions (including the overlapping peptide sequences) were exclusively found in apoplastic water-soluble fraction (AWF NaCl)-extracted isoenzyme P1 from manganese-treated plants Vigna unguiculata
β-polypeptides from Bradi4g28220.1 were identified with theoretical pI of 7.2 Brachypodium distachyon
evolutionary analysis of 500 sequences with E-value < e-102 indicates that Tyr207 is an extremely well-conserved residue
sequential extraction relying on solubility properties enabled deeper and more comprehensive description of protein composition
root mitochondria of pea showed only two bands Pisum sativum
BTL homologue is membrane protein of c. 31 kDa Vitis vinifera
non-reducing SDS-PAGE in C59S and C84S variants showed only one monomer Pisum sativum
PsPrxIIF/Trx-o interaction was detected in mitochondrial matrix extracts fractionated by size exclusion chromatography Pisum sativum
peptide SIGDGVQFLNR did not match previously reported SuSy sequences Medicago truncatula
SSIIa apparent molecular weight matches predicted value of the cDNA sequences Zea mays
LC-MS/MS method used for simultaneous determination of NO2-Tyr and Tyr
70 kDa and 50 kDa polypeptides amino acid sequences match N-terminal and C-terminal of encoded polypeptide Capsicum
(ASUS1, atsus1, SUS1, SUSY1, AT5G20830) tryptic peptide SIGNGVQFLNR was chosen for screening for isoform-specific masses Medicago truncatula
hyperphosphorylation state of M. truncatula (ASUS1, atsus1, SUS1, SUSY1, AT5G20830) makes understandable that the conserved Ser11 phosphopeptide has been described several times Medicago truncatula
clone 3 recombinant protein was characterized as 56.3 kDa, 525 amino acid protein Zea mays
six spots corresponding to non-maturated forms were detected around 52 kDa Brachypodium distachyon
proteomic approaches brought two notable results about Brachypodium grain composition Brachypodium distachyon
DBE and (ATPHO1, PHO1, AT3G23430) activity bands were visualized by native-PAGE which contains potato amylopectin as a substrate Oryza sativa
SSIIa, SSIIIa, and SSIVb migrated to positions corresponding to multiple, different, molecular weights Oryza sativa
11S globulin type proteins appears to be major form of storage proteins in mature Brachypodium distachyon grain Brachypodium distachyon
mutated PROAtCAPE1–eYFP in three independent CAPE1ox CNAD transgenic lines only single band with molecular weight of 45.76 kDa detected Arabidopsis thaliana
fractions from gel filtration contained (ATPRXIIF, PRXIIF, AT3G06050) and Trx-o Pisum sativum
minor dimeric form appears after SEC Arabidopsis thaliana
His6-tagged SrUCPB has electrophoretic mobility similar to native SrUCPA Symplocarpus racenosus
phosphorylated GBSS identity demonstrated by electrophoretic mobility comparison Zea mays
denaturing SDS-PAGE analyzed purity of FcpA His Phaeodactylum tricornutum
prominent single negative band was attributed to Chl a Phaeodactylum tricornutum
protein of 453 amino acids (first cDNA) has theoretical molecular mass of 51.2 kDa Glycine max
mature C. chinense (AtPR4, HEL, PR-4, PR4, AT3G04720) protein has molecular mass of 13480.92 Da Capsicum chinense
vacuolar invertase (PhINV) was protein isoform Petunia×hybrida
in vitro synthesized SrUCPA exhibited the same electrophoretic mobility as uncoupling protein in skunk cabbage mitochondria Symplocarpus racenosus
tobacco leaf proteomics has been used to study trichome proteome
hexameric C84S variant of PsPrxIIF showed different electrophoretic mobility in SDS-PAGE Pisum sativum
peak at 12.9 ml under non-reducing conditions corresponded to hexameric (ATPRXIIF, PRXIIF, AT3G06050) Pisum sativum
internal granule-associated proteins were stained using phospho-protein specific dye
additional form of BEIIb is distinguishable by altered migration
Nb AMAN close to predicted molecular weight of 114 kDa Nicotiana benthamiana
Nb PNGaseA might be full-length protein (62 kDa) Nicotiana benthamiana
Nb (scpl50, AT1G15000) (NbL15g24880) mostly in gel Slice 11 at 50 kDa Nicotiana benthamiana
catalytic triad of eight GELPs consists of catalytic Ser residue in N terminal domain and catalytic Asp and His residues in C terminal domain Nicotiana benthamiana
PROTOMAP dataset mined for secreted proteases with autoinhibitory prodomain Nicotiana benthamiana
BEI activity was also observed in fractions 2–9, corresponding to BEI protein between 200 kDa and >700kDa Oryza sativa
outlier NAB domain-like sequence from (AT1G48405) was used as negative control Arabidopsis thaliana
liquid chromatography-tandem mass spectrometry is protein analysis method
in silico study chosen for identification of Tyr residue/s potentially nitrated in SAHH
quantitative proteomics identified N-glycosylation sites Colletotrichum graminicola
131 apoplastic proteins positive for antimicrobial activity had average amino acid sequence length of 499 Albugo candida
C-terminal fibronectin III-like domain of Nb BXYL detected at 75 kDa as part of full-length protein Nicotiana benthamiana
Nb (EDA2, AT2G18080) also shows N-terminal peptides at 28 kDa Nicotiana benthamiana
cleaved SCPLs many carry dibasic motifs
PROTOMAP dataset contains peptographs of 96 glycosyl hydrolases (GHs) Nicotiana benthamiana
recombinant proteins were analyzed by SDS-PAGE Escherichia coli
structure-function studies focused on C-terminal domain of ALMT (aluminum-activated malate transporter) proteins
recombinant (AtPAO5, PAO5, AT4G29720) protein has a mean value of 0.95 mole of FAD per mole of enzyme Arabidopsis thaliana
overlap of emission spectra after excitation at 440, 475 and 500 nm at 5°C indicates no free pigments in preparation Chlamydomonas reinhardtii
overlapping proteins were analyzed using InterProScan Beta vulgaris
SignalP-predicted signal peptides highlighted in peptograph
five additional SCPLs indicating not processed Nicotiana benthamiana
β-galactosidase-1 (Nb (BGAL1, AT3G13750) NbL07g00850, family GH35) carries galactose-binding lectin domain (PF02140) at C terminus Nicotiana benthamiana
nine cystatins have molecular mass in the range of 10.8–16.1 kDa Nicotiana tabacum
Dr. Henry N. Higgs performed analytical ultracentrifugation experiments with (AFH1, AHF1, ATFH1, FH1, AT3G25500)
peroxynitrite-treated recombinant APX was subjected to trypsin digestion followed by MALDI-TOF/TOF mass spectrometry Pisum sativum
heterologous bicarbonate transporter function and location in chloroplasts requires span of experimental measurements Nicotiana tabacum
ictB fused with N-terminal plastid transit sequence and transformed into host genome growth benefits observed without confirmation of IctB protein synthesis or cellular localization C3 plants
experimental rigor in analysing cellular localization and functionality of recombinant membrane proteins is needed in analysing recombinant membrane proteins targeted to leaf plastids
BN-PAGE minimizes influence of differences in isoelectric point of individual isozymes Oryza sativa
QrSUT1 has ORF of 500 amino acids and calculated mol. wt of 53.2kDa Quercus robur
cell-wall bound or apoplastic invertase (cwInv) is distinguished by solubility, subcellular localization, pH optima, and isoelectric point
TSA (thermal shift assay) shows the melting temperature of purified trimer interface mutant proteins (ΔC and YV-EE) and catalytic site mutant proteins (S214A) of DM3 variants
proteomic composition of plasmodesmata (PD) has been characterized new data on protein composition
plant SUN-domain proteins were initially described in Arabidopsis thaliana and Zea mays Arabidopsis thaliana; Zea mays
NtCYS3 has molecular mass of ~28.1 kDa with a C-terminal extension Nicotiana tabacum
strongest BEI activity was found in fractions 10–12 which contained the largest amounts of BEI protein Oryza sativa
eluate at 13.5 ml from gel filtration contained both Trx-o and PsPrxIIF Pisum sativum
plastid proteases have been identified by biochemical and genetic analyses, proteomic analysis, prediction from EST databases and expression profiling
55 kDa band corresponds to size of uncleaved glutelin precursor Oryza sativa
sequential extraction of storage proteins achieved more detailed description of Brachypodium distachyon grain proteome Brachypodium distachyon
13 sequences containing NAB domain range in predicted size from 25 to 199 kDa Arabidopsis thaliana
two spots corresponding to basic polypeptides were reported in Table 2 Brachypodium distachyon
polypeptide of 551 aa has predicted molecular weight of 62.9 kDa Olea europaea
BN-PAGE reflects molecular weight of the protein complexes Oryza sativa
transit peptide (TP) interferes with further biochemical studies Arabidopsis thaliana
fractions with the highest enzymatic activity generally correlated with strongest signals obtained by SDS–PAGE and western blotting Oryza sativa
most of the BEIIa was present at monomeric molecular weight Oryza sativa
FLAGELLIN SENSING 2 (ATFLS2, FLS2, AT5G63580) mobility was examined by variable angle-total internal reflection fluorescence (VA-TIRF) microscopy Arabidopsis thaliana
Five scans were accumulated and spectrum of buffer without protein was subtracted
fusion protein from transgenic plants gave single band with molecular mass of 105 kDa Arabidopsis thaliana
predicted proteins from env ORF show remarkable differences in amino acid composition Beta vulgaris
lipolytic bands have molecular weights ranging from 90 kDa to 30 kDa
recombinant (AtPAO5, PAO5, AT4G29720) protein has electrophoretic homogeneity of 70% protein purity Arabidopsis thaliana
SSI and SSIIIa activities were visualized using non-denaturing gels containing oyster glycogen as a primer Oryza sativa
strong (ATPHO1, PHO1, AT3G23430) activity was seen in fractions 5–9 Oryza sativa
Rubisco activase sequences have been identified in Nicotiana attenuata leaves Nicotiana attenuata
high-performance liquid chromatography with tandem mass spectrometry (LC-MS/MS) was used to study tyrosine nitration (NO2-Tyr) content Helianthus annuus
recombinant APX showed molecular mass of ~49kDa Pisum sativum
BicA oligomeric assemblies identified in concentrated tob BicA chloroplast membrane protein preparations Nicotiana tabacum
zymogram analysis was based on Pho a stimulation assay Oryza sativa
molecular weight distribution of BEIIa was narrower than that of BEIIb or BEI Oryza sativa
starch biosynthetic enzymes in high molecular weight complexes was confirmed by western blotting of a BN-PAGE gel Oryza sativa
recombinant (AtPAO5, PAO5, AT4G29720) protein has emission peak at 530nm Arabidopsis thaliana
fluorescence-based localization studies and novel expression studies with quantitative real-time PCR (qRT-PCR) are still necessary to prove OsAMT1;1 localization in plasma membrane and NH4+ responsiveness of expression Oryza sativa
CsCCD2 and CsALDH3I1 were expected in insoluble fraction Crocus sativus
N-terminal blocking of acidic AtFNR spots prompted search for possible N-terminal modifications Arabidopsis thaliana
incomplete separation capacity of BN-PAGE indicates presence of both AtFNR isoforms and (AtTic62, Tic62, AT3G18890) and (TROL, AT4G01050) in all complexes Arabidopsis thaliana
AtFNR is not one of those glycoproteins Arabidopsis thaliana
bands of ~80 and ~60 kDa correspond to sizes previously reported for H+-ATPase Hordeum vulgare
further work is necessary to elucidate structure of Pro-rich region and its glycosylations Arabidopsis thaliana
decreased seed ferritin abundance observed in the nramp3-4 mutant is not due to protein loading variations or general impairment of seed protein pattern Arabidopsis thaliana
plant metabolite transporters have not yet been identified at molecular level
two-dimensional gel electrophoresis of latex proteins confirmed relative abundance of Codeinone reductase (COR) isoforms in laticifers Papaver somniferum
(ATCAL4, CML12, TCH3, AT2G41100) has 324 amino acids with six EF-hands Arabidopsis thaliana
phospho-mimetic mutant CCaMK S337D was constructed and used in calmodulin binding and CCaMK kinase activity assays Lotus japonicus
phloem protein 1 (PP1) has molecular weight of 95 kDa Cucurbita maxima
recombinant (AtPAO5, PAO5, AT4G29720) protein has flavin cofactor at 380nm and 450nm excitation peaks Arabidopsis thaliana
phloem filament protein (PP1) was isolated in large quantities thorough biochemical analysis Cucurbitaceae
immunolocalization of PTMs using PTM-specific antibodies revealed combinations of different PTMs on individual MTs
characteristics of the other five proteins did not coincide with results from 2-DE Oryza sativa
BEI was present over broad range of molecular sizes Oryza sativa
specific amino acid residues that correlate with NDPS1 or zFPS activity role in determining substrate and product specificity was confirmed through site-directed mutagenesis Solanum habrochaites
knowledge on the function and biochemical properties of glutaredoxins (GRXs) in photosynthetic organisms has rapidly increased during the last 5 years recent period
MS or MS/MS alone will not fully reveal structural features of recalcitrant protein such as location of Hyp residues Arabidopsis thaliana
xyloglucan-specific endo-(1 → 4)-β-glucanase preparation subjected to SDS-PAGE
cleaved SCPLs have longer linker regions (32–65 residues)
P-loop NTPase domain was the second most abundant domain observed (180 predictions) Beta vulgaris
Cluster III has no TBL protein belonging to described TBL protein or corresponding mutants Arabidopsis thaliana
secondary structure of WT and mutants of AvrPib were evaluated spectroscopically by Cd Escherichia coli
CD spectra were recorded with 4 μM sample of SLAH3-GFP using Jasco J810 instrument Arabidopsis thaliana
full-length tryptic peptide carrying RCL was not detected in peptide mapping
melting temperature (Tm) of (ATRBP47B, RBP47B, AT3G19130) calculated at 55.6°C
monomer of (NTRC, AT2G41680) has estimated molecular mass of 66 ± 5 kDa
pKa values of all ionizable residues of (SLAH3, AT5G24030) could be obtained with PROPKA 3.0 Arabidopsis thaliana
ten spots corresponding to acidic polypeptides were detected around 30 kDa or less Brachypodium distachyon
PDE treatment of denatured recombinant (AtPAO5, PAO5, AT4G29720) induces a 10-fold increase in fluorescence intensity Arabidopsis thaliana
EMSA using BSA as a reference protein substrate was applied to test whether the hydrolysis of oleuropein by OeGLU was able to promote in vitro a similar protein cross-linking activity Olea europaea
different (ATBRI1, BIN1, BRI1, CBB2, DWF2, AT4G39400) fragments were tested in analytical size-exclusion chromatography experiments
other protein spots appeared to be hypothetical proteins Oryza sativa; Arabidopsis thaliana
non-denaturing PAGE showed single band with high molecular mass Populus tomentosa
Soret/A280 ratios of proteins were approximately 2.7 Lotus japonicus
polypeptides detected in IP sample had similar electrophoretic mobility and relative intensity to polypeptides detected in P-170 fraction Medicago truncatula
CP29 and CP26 in PSI–LHCI–LHCII supercomplexes are present in different amounts Chlamydomonas reinhardtii
absorption spectrum of Lhcb fraction associated with PSI was determined by comparing spectra of PSI–LHCI–LHCII and PSI–LHCI after normalization to Chl content Chlamydomonas reinhardtii
phyAΔ5 after saturating red (R) irradiation migrated with apparent molecular weight (aMW) of 116 and 94 kDa Escherichia coli
Chlamydomonas reinhardtii wild-type 137c, (AtPGR5, PGR5, AT2G05620) and pgrl1 cells were subjected to immunoblot analysis of proteins involved in photosynthesis and respiratory processes Chlamydomonas reinhardtii
SALAD database facilitates protein motif and interaction analysis Oryza sativa; Arabidopsis thaliana
Rice DB allows retrieval of protein properties Oryza sativa
lower resolution map of small particle prevents antenna complexes cannot be fitted unambiguously Chlamydomonas reinhardtii
SrUCPA is a representative of plant UCPs Symplocarpus racenosus
JIM11-labelled extensins are mainly found above 223 kDa molecular weight Nicotiana tabacum
(SDS, AT1G14750) loading dye is used in SDS-PAGE
Ca2+-binding activity of many EF-hand proteins further study is needed to verify Arabidopsis thaliana
(APC1, AT5G61810) and (AT3G03430) smallest of EF-hand proteins Arabidopsis thaliana
CPKs range from 453 to 646 amino acids Arabidopsis thaliana
(CTPS3, AT4G02120) underwent detailed biochemical characterization Arabidopsis thaliana
eukaryotic members of AtCN-PDE1-like subfamily have not been functionally characterized
phyAΔ5 alone after saturating far-red (FR) irradiation migrated with apparent molecular weight (aMW) of 104 kDa Escherichia coli
broad minimum in CD spectra of wild-type or E46K mutant was typical of β-strand-rich protein Escherichia coli
Cr P II has calculated trimer size of 51 kDa
overall protein content of Brachypodium grain approaches level described in oat kernels Brachypodium distachyon; Avena sativa
purified recombinant EsM1Pase2 displayed several other bands of different sizes Ectocarpus siliculosus
hydrolytic activity of PUL found in fractions 2–13 Oryza sativa
predicted molecular mass of AtCRL∆TM (aa 43–239) is approximately 23 kDa
biochemical characterization of transporter affinities and specificities is commonly performed in heterologous systems
liquid chromatography-tandem mass spectrometry identified mature SlIDA as a 14-mer EPIP peptide Solanum lycopersicum
Purified SLAH3-GFP fusion protein was dissolved in buffer containing 10 mM NaH2PO4, 67 mM Na2SO4, 10% (v/v) glycerol, 0.03% (w/v) DDM pH 6.3 Arabidopsis thaliana
proteomics, immunolocalization, comparative genomics, phylogenetics and structural homology analysis used to investigate evolutionary history and function of diatom adhesive proteins
Nb GMCO also at 50 kDa with peptides from N-terminal half Nicotiana benthamiana
membrane-bound and soluble proteins were isolated, separated by 2D SDS-PAGE Arabidopsis thaliana
SS4N-YFP has expected molecular weight of 85 kD Arabidopsis thaliana