| PAL (phenylalanine ammonia-lyase) |
is upregulated in |
(FER, AT3G51550) mutants roots |
Solanum lycopersicum |
| DIR19 (dirigent protein 19) |
is increased in |
(FER, AT3G51550) mutants roots |
Solanum lycopersicum |
| ralf2 mutant |
showed increase in |
lignin content |
Solanum lycopersicum |
| dir19 mutants |
display opposite trend in |
lignin content in roots |
Solanum lycopersicum |
| apyrase-suppressed mutants |
have much more extensive |
lignin formation |
Arabidopsis thaliana |
| SvSH1 |
regulates |
(ATECS1, AtGSH1, CAD2, GSH1, GSHA, PAD2, RAX1, RML1, AT4G23100) |
Setaria viridis |
| (ATMYB58, MYB58, AT1G16490) 63 transcription factor overexpression |
results in |
ectopic lignin deposition |
Arabidopsis thaliana |
| (ATMYB58, MYB58, AT1G16490) /63 overexpression |
leads to |
ectopic deposition of lignin |
Arabidopsis thaliana |
| ralf2 dir19 double mutants |
exhibit reduced |
lignin content compared to ralf2 mutants |
Solanum lycopersicum |
| 4CL |
is upregulated in |
ralf2 and (FER, AT3G51550) mutants |
Solanum lycopersicum |
| AtBLH6 orthologs |
are required to enhance |
lignin biosynthesis |
Oryza sativa; Populus sp. |
| eQTL mapping |
identifies |
genomic hotspots involved in lignin biosynthesis |
Populus |
| RALF-FER signaling |
modulates |
lignin content in tomato roots |
Solanum lycopersicum |
| (FER, AT3G51550) dir19 double mutants |
exhibit reduced |
lignin content compared to (FER, AT3G51550) mutants |
Solanum lycopersicum |
| peroxidase gene |
is not upregulated by |
AMF in wild rice |
Oryza rufipogon |
| low and non-lethal concentrations of H2S |
upregulates |
genes related to lignin biosynthesis |
|
| defects in cell type distribution at the stem |
related to |
defects in enzymatic pathway components essential for lignin biosynthesis |
Arabidopsis thaliana |
| (ATMYB46, MYB46, AT5G12870) 83 transcription factor overexpression |
results in |
ectopic lignin deposition |
Arabidopsis thaliana |
| phenylpropanoids |
are |
core components of lignin |
Arabidopsis thaliana |
| DIR19 (dirigent protein 19) |
is required for regulation of |
lignin accumulation by RALF2-FER signaling |
Solanum lycopersicum |
| cinnamyl alcohol dehydrogenase gene |
is significantly upregulated by |
AMF in domesticated rice |
Oryza sativa |
| (CYP98A3, REF8, AT2G40890) mutant |
has substantially reduced |
overall lignin levels |
Arabidopsis thaliana |
| p-coumarate 3-hydroxylase (C3H) |
is |
lignin biosynthesis gene |
|
| monolignol-ferulic acid coupling products |
are reduced in |
ccr1-6 mutants compared to wild-type |
Arabidopsis thaliana |
| ccr1-6 mutants and (ATCAD4, CAD, CAD-C, CAD4, AT3G19450) -d double mutants |
have been shown to have similarly decreased |
lignin contents |
Arabidopsis thaliana |
| (ATCAD4, CAD, CAD-C, CAD4, AT3G19450) -d double mutants |
had S/G ratio of |
0.081 |
Arabidopsis thaliana |
| p-coumarate in cell walls of Poaceae |
acylates |
lignin |
|
| lignification in anthers |
is initiated by |
activation of CCR by auxin |
Oryza sativa |
| miR397 |
has low levels in |
pollen |
|
| UDP-glycosyltransferases (UGTs) expression |
is affected in |
(H3.3, HTR8, AT5G10980) K27A |
Arabidopsis thaliana |
| cinnamyl alcohol dehydrogenase 2 (ATECS1, AtGSH1, CAD2, GSH1, GSHA, PAD2, RAX1, RML1, AT4G23100) |
is |
direct target of SvSH1 |
Setaria viridis |
| (AtMYB36, MYB36, AT5G57620) |
directly binds to and positively regulates transcription of |
AtESB1 |
Arabidopsis thaliana |
| lignin biosynthetic pathway |
is strongly but differentially affected by |
AMF in wild and domesticated rice |
Oryza sativa; Oryza rufipogon |
| upregulation of lignin-related genes |
is seen as |
stress response |
Oryza sativa |
| multiple β-glucosidases (BGLUs) expression |
is affected in |
(H3.3, HTR8, AT5G10980) K27A |
Arabidopsis thaliana |
| aromatic amino acids (AAAs) |
are precursors for |
lignin |
|
| 4CL (4-coumarate-CoA ligase) |
is upregulated in |
ralf2 mutants roots |
Solanum lycopersicum |
| (FER, AT3G51550) mutant |
showed upregulation of genes associated with |
monolignol synthesis and polymerization |
Solanum lycopersicum |
| Intermated (B73, CHL6, CNX, CNX1, SIR4, AT5G20990) × Mo17 recombinant inbred maize population |
used to define |
quantitative trait loci (QTL) for lignin abundance |
Zea mays |
| (AR2, ATR2, AT4G30210) mutants |
show reduced abundance of |
G(8-O-4/8-5)phenylpropenoic acids/esters |
Arabidopsis thaliana |
| differences in lignin composition and phenolic metabolite levels between (AR2, ATR2, AT4G30210) mutants and wild-type plants |
can logically be explained by |
lower (ATC4H, C4H, CYP73A5, REF3, AT2G30490) C3H1, and F5H1 activities in (AR2, ATR2, AT4G30210) mutants |
Arabidopsis thaliana |
| lignin biosynthetic pathway |
has been |
largely elucidated |
Arabidopsis thaliana |
| lignin of ccr1-6 mutants |
had S/G ratio of |
0.48 |
Arabidopsis thaliana |
| lignin biosynthesis pathway |
was activated in outer part of root under hydrogen sulphide and stagnant conditions |
lignin deposition |
|
| Svsh1 knockout in ME034 background |
results in |
differential expression of eight lignin-related genes |
Setaria viridis |
| peroxidase gene |
is significantly upregulated by |
AMF in domesticated rice |
Oryza sativa |
| (AtMYB43, MYB43, AT5G16600) |
directly activates |
lignin biosynthesis genes |
|
| (ATMYB63, MYB63, AT1G79180) N-OE plants |
show increased |
lignin levels in roots |
Solanum lycopersicum |
| ralf2 mutants |
show increased |
lignin content in roots |
Solanum lycopersicum |
| (FER, AT3G51550) mutant |
showed increase in |
lignin content |
Solanum lycopersicum |
| AMF inoculation and domestication status |
has interactional effect on |
lignin levels |
Oryza sativa; Oryza rufipogon |
| lignin biosynthesis-related genes |
were commonly upregulated in both hydrogen sulphide and stagnant conditions |
lignin biosynthesis pathway |
|
| AMF |
reduced |
lignin concentrations in lateral roots of wild rice |
Oryza rufipogon |
| Liu et al. (2022) |
reported |
lignin biosynthetic genes regulated by SvSH1 |
Setaria viridis |
| (FER, AT3G51550) mutants |
show increased |
lignin content in roots |
Solanum lycopersicum |
| DIR19 (dirigent protein 19) |
is increased in |
(ATMYB63, MYB63, AT1G79180) N-OE#12 plants |
Solanum lycopersicum |
| Lignin synthesis and vessel development |
are regulated by |
subgroup of MYB family members |
|
| alteration of the activity of genes involved in monolignol synthesis or polymerization |
can impact |
lignin abundance |
|
| OsCCR18 |
may compensate for |
OsCCR14 reduction |
Oryza sativa |
| PAL (phenylalanine ammonia-lyase) |
is upregulated in |
ralf2 mutants roots |
Solanum lycopersicum |
| m/z 120 (4-vinylphenol) |
is derived from |
p-coumaric acid |
Zea mays |
| NbPAT silenced plants |
showed |
significant reduction in lignin content in leaves and stems |
Nicotiana benthamiana |
| (MED33A, MED5A, RFR1, AT3G23590) (MED33B, MED5B, REF4, AT2G48110) c3h triple mutants |
had lignin amount restored to |
wild-type levels |
Arabidopsis thaliana |
| monolignols (MLs) |
are enzymatically oxidized by |
peroxidases and laccases |
|
| pathogen invasion |
leads to upregulation of |
lignin biosynthetic and deposition genes |
|
| WRKY proteins |
are found in |
QTL6 for lignin/4-vinylphenol traits |
Zea mays |
| homologs for almost every gene in the phenylpropanoid synthesis pathway |
are present within |
at least one of the lignin abundance- or saccharification yield-related QTL regions |
Zea mays |
| sinapyl alcohol |
gives rise to |
syringyl (S) units |
|
| VvABF2 |
seems to affect |
lignin biosynthesis by stimulation of (ATCAD4, CAD, CAD-C, CAD4, AT3G19450) and laccase genes |
Vitis vinifera |
| genes involved in monolignol synthesis or polymerization |
are differentially expressed at least 5-fold in B73 as compared with Mo17 in |
lignin/4-vinylphenol QTL |
Zea mays |
| reduced abundance of oligolignols in (AR2, ATR2, AT4G30210) mutants |
indicates reduced flux toward |
coniferyl alcohol |
Arabidopsis thaliana |
| (AR2, ATR2, AT4G30210) mutants |
show increased |
H units in lignin |
Arabidopsis thaliana |
| isoxaben |
induces |
ectopic lignification |
Arabidopsis thaliana |
| enrichment in p-coumaryl alcohol |
results in |
shorter lignin polymers |
Arabidopsis thaliana |
| CB5 and (ATC4H, C4H, CYP73A5, REF3, AT2G30490) interaction |
was detected in |
Arabidopsis cell cultures grown under standard conditions |
Arabidopsis thaliana |
| (CYP98A3, REF8, AT2G40890) pOpON transgenic plants |
accumulate |
p-hydroxyphenyl lignin units |
Arabidopsis thaliana |
| (ATGRP8, CCR1, GR-RBP8, GRP8, RBGA6, AT4G39260) mutants |
show |
overall reduction in lignin deposition |
Arabidopsis thaliana |
| (ADT1, AtADT1, AT1G11790) /4/5 triple mutant |
displayed |
visual evidence of bolting stem structural weakening |
Arabidopsis thaliana |
| (ATCAD4, CAD, CAD-C, CAD4, AT3G19450) clade |
comprises |
Oryza sativa OsCAD2; Populus tremula × Populus alba PtrCAD1; Arabidopsis (ATCAD4, CAD, CAD-C, CAD4, AT3G19450) and (ATCAD5, CAD-5, CAD5, AT4G34230) Zea mays ZmCAD2; Sorghum bicolor SbCAD2 |
Oryza sativa; Populus tremula; Populus alba; Arabidopsis thaliana; Zea mays; Sorghum bicolor |
| increasing expression of poplar ferulate-5-hydroxylase gene |
results in lignin that is almost entirely |
syringyl (S) lignin |
Populus spp. |
| (ATMYB30, MYB30, AT3G28910) |
shows expression differences of 5-fold or greater in |
QTL6 for lignin/4-vinylphenol traits |
Zea mays |
| C3′H deficiency in (CYP98A3, REF8, AT2G40890) |
blocks formation of |
syringyl lignin |
Arabidopsis thaliana |
| lignin-linked p-coumarate |
occurs exclusively on |
hydroxyl group on γ-carbon of lignin unit side chains |
|
| free radicals from monolignol oxidation |
polymerize nonenzymatically to form |
lignin |
|
| OsCCR14 |
is the closest homolog to |
(ATCCR1, CCR1, IRX4, AT1G15950) |
Oryza sativa; Arabidopsis thaliana |
| (ADT4, AT3G44720) knockout |
resulted in |
slightly higher lignin stem content |
Arabidopsis thaliana |
| (ADT1, AtADT1, AT1G11790) /4/5 triple mutant |
displayed |
approximately 50% reduction in lignin content |
Arabidopsis thaliana |
| genes in the (ATCAD4, CAD, CAD-C, CAD4, AT3G19450) clade |
are true CADs and are involved in |
monolignol synthesis |
|
| reduced (HCT, AT5G48930) activity |
results in |
relative increase in H units |
Arabidopsis thaliana |
| lignin |
is synthesized by |
polymerization of hydroxycinnamyl alcohols or monolignols |
|
| recombinant (ATCAD8, CAD-B2, ELI3, ELI3-2, AT4G37990) |
had slight activities against |
intermediates of the lignin biosynthetic pathway |
Arabidopsis thaliana |
| reduced S/G ratio in (AR2, ATR2, AT4G30210) mutants |
strongly suggests |
F5H1 activity is negatively affected by reduced (AR2, ATR2, AT4G30210) activity |
Arabidopsis thaliana |
| (CYP98A3, REF8, AT2G40890) mutant |
has nearly eliminated |
guaiacyl and syringyl lignin subunits |
Arabidopsis thaliana |
| individual CAD-genes |
are not |
rate-limiting |
Arabidopsis thaliana |
| all (ATCAD4, CAD, CAD-C, CAD4, AT3G19450) genes |
may not all |
encode bona fide CADs and contribute to monolignol formation |
Arabidopsis thaliana |
| cation-independent caffeic acid OMT (COMT) |
is involved in |
formation of sinapoyl residues and S-lignin from 5-hydroxyferuloyl derivatives |
Arabidopsis thaliana |
| coniferaldehyde end-groups |
are present in low amounts in |
native lignins |
|
| (AR2, ATR2, AT4G30210) mutant |
results in reduction of |
syringyl units |
Arabidopsis thaliana |
| vessel-specific expression of (ATC4H, C4H, CYP73A5, REF3, AT2G30490) |
restored growth phenotype even though |
overall lignin levels were still reduced compared with wild type |
Arabidopsis thaliana |
| Arabidopsis reduced epidermal fluorescence3 (ATC4H, C4H, CYP73A5, REF3, AT2G30490) mutant |
display |
severe growth defects and sterility |
Arabidopsis thaliana |
| C3'H activation |
depends on |
timing during plant development |
Arabidopsis thaliana |
| (ATCCR1, CCR1, IRX4, AT1G15950) |
is found to play a significant role in |
lignification |
Arabidopsis thaliana |
| mass-to-charge ratio (m/z) fragments |
are diagnostic for |
hydroxycinnamic acid |
|
| differentially expressed lignin biosynthetic enzymes |
predominate in |
lignin-related QTL |
Zea mays |
| substrates of p-COUMARATE 3-HYDROXYLASE1 (C3H1) |
accumulate in |
(AR2, ATR2, AT4G30210) mutants |
Arabidopsis thaliana |
| C3′H deficiency in (CYP98A3, REF8, AT2G40890) |
blocks formation of |
guaiacyl lignin |
Arabidopsis thaliana |
| dexamethasone (dex) induction of C3′H in (CYP98A3, REF8, AT2G40890) pOpON stems |
affects |
lignin deposited in (CYP98A3, REF8, AT2G40890) mutant |
Arabidopsis thaliana |
| ADT knockout mutants |
are associated with |
different degrees of reduction of lignin content |
Arabidopsis thaliana |
| (ADT4, AT3G44720) /5 double mutant |
displayed |
further reduction in lignin content of approximately 38% |
Arabidopsis thaliana |
| (ADT3, PD1, AT2G27820) /4/5/6 quadruple mutant |
was unable to maintain |
bolting stem structural integrity |
Arabidopsis thaliana |
| genes involved in lignin biosynthesis |
are known |
lignin biosynthesis pathway |
|
| ATR2-deficient plants |
have |
different lignin composition |
Arabidopsis thaliana |
| down-regulation or mutation of genes and enzymes early in the pathway |
leads to |
drastic lignin reduction |
Arabidopsis thaliana |
| lignin deficiency |
may directly impact |
plant growth |
|
| de novo lignin biosynthesis |
is not essential for |
germination or cotyledon development |
Arabidopsis thaliana |
| p-hydroxyphenyl (H), guaiacyl (G), and syringyl (S) units |
are derived from |
p-coumaryl, coniferyl, and sinapyl alcohol |
|
| (ATCCR1, CCR1, IRX4, AT1G15950) gene |
encodes for |
CCR designated as (ATCCR1, CCR1, IRX4, AT1G15950) |
Arabidopsis thaliana |
| (ADT5, AT5G22630) |
had lignin contents reduced the most under |
long-day growth/development |
Arabidopsis thaliana |
| reduced accumulation of lignin in stems of plants silenced for NbPAT |
would be |
direct consequence of prioritized use of Phe pool toward protein biosynthesis |
Nicotiana benthamiana |
| recombinant (ATCAD4, CAD, CAD-C, CAD4, AT3G19450) or (ATCAD5, CAD-5, CAD5, AT4G34230) enzymes |
have higher activities against |
intermediates of the lignin biosynthetic pathway |
Arabidopsis thaliana |
| precise channeling of individual precursors through metabolons |
would be |
superior mode of control |
|
| (CYP98A3, REF8, AT2G40890) |
hydroxylates |
quinate and shikimate esters of 4-coumaric acid |
Arabidopsis thaliana |
| ccr1g mutant |
displays reduced lignin staining in |
xylem vessels |
|
| ccr1g mutant |
displays absent lignin staining in |
fibers |
|
| sinapyl alcohol |
is precursor of |
S lignin units |
Arabidopsis thaliana |
| ccc mutant |
releases very little amounts of |
monomers |
|
| genome-wide epistatic interaction targets of Rc and (ATIPT5, IPT5, AT5G19040) |
include |
target genes involved in phenylpropanoid and lignin biosynthesis, mevalonate pathway, isoprenoid metabolism, and flavonoid biosynthesis |
Oryza sativa |
| functionally characterized enzymes of the core phenylpropanoid and lignin biosynthesis routes |
derive from |
lineage-specific radiations |
|
| MeCAD15 enzyme activity |
may be influenced by |
MeRAV5 |
Manihot esculenta |
| (AR2, ATR2, AT4G30210) |
ability to donate electrons to |
(ATC4H, C4H, CYP73A5, REF3, AT2G30490) |
Arabidopsis thaliana |
| xylem region |
is more enriched in |
G units |
Arabidopsis thaliana |
| (AtbZIP, bZIP, AT1G68880) transcription factors |
are found in |
QTL5 for lignin/4-vinylphenol traits |
Zea mays |
| ARABIDOPSIS THALIANA CYTOCHROME P450 REDUCTASE2 (AR2, ATR2, AT4G30210) |
is hypothesized to be preferentially involved in providing electrons to |
p-COUMARATE 3-HYDROXYLASE1 (C3H1) |
Arabidopsis thaliana |
| down-regulation of (AR2, ATR2, AT4G30210) |
affects |
lignin structure |
Arabidopsis thaliana |
| atr2-2 mutant |
shows decreased |
G units in lignin |
Arabidopsis thaliana |
| β-syringyl ether linkages |
is reduced in |
(AR2, ATR2, AT4G30210) mutants |
Arabidopsis thaliana |
| oligolignols and hexosylated oligolignols |
are also reduced in |
(ATGRP8, CCR1, GR-RBP8, GRP8, RBGA6, AT4G39260) -6 ProSNBE compared to wild-type |
Arabidopsis thaliana |
| sinapyl alcohol |
produces |
syringyl (S) lignin units |
|
| (ATCAD4, CAD, CAD-C, CAD4, AT3G19450) and (ATCAD5, CAD-5, CAD5, AT4G34230) |
are confirmed to be CADs of |
lignin biosynthesis pathway in floral stems |
Arabidopsis thaliana |
| (AtMAGL3, CSE, LysoPL2, AT1G52760) |
is involved in |
lignification |
Arabidopsis thaliana |
| (AR2, ATR2, AT4G30210) mutants |
show decreased |
S/G ratio |
Arabidopsis thaliana |
| reduced C3H activity |
results in |
relative increase in H units |
Arabidopsis thaliana |
| (AR2, ATR2, AT4G30210) electron donation |
is important for |
lignifying cells |
Arabidopsis thaliana |
| roots |
accumulate |
lignin |
|
| modulating enzyme activities in lignin biosynthesis pathway |
alters |
abundance of lignin biosynthesis substrates |
|
| 4-COUMARATE : COENZYME A LIGASE (4CL) |
is |
lignin biosynthesis gene |
|
| alternative approaches to achieve vessel-specific lignification in poplar |
via |
perturbing lignification specifically in fibers |
Populus spp. |
| nontraditional monomers |
can be incorporated into |
lignin in wild-type and genetically modified plants |
|
| OsCCR14 |
could play important roles in |
lignification process |
Oryza sativa |
| (AtATR1, ATMYB34, ATR1, MYB34, AT5G60890) |
follows similar expression profile to |
lignin biosynthetic genes |
Arabidopsis thaliana |
| partial restoration of lignification in xylem |
will lead to |
further decrease in S/G ratio in (ATGRP8, CCR1, GR-RBP8, GRP8, RBGA6, AT4G39260) -6 ProSNBE lines |
Arabidopsis thaliana |
| OsFBK1 knockdown transgenics |
displayed |
more lignin accumulation in root tissues |
Oryza sativa |
| (ADT3, PD1, AT2G27820) /4/5 triple mutant |
displayed |
approximately 50% reduction in lignin content |
Arabidopsis thaliana |
| (AR2, ATR2, AT4G30210) mutants |
show reduced abundance of |
hexosylated oligolignols |
Arabidopsis thaliana |
| TRANSALDOLASE2 |
is involved in |
lignification |
Arabidopsis thaliana |
| RNA interference (RNAi) plants in which hydroxycinnamoyl-CoA shikimate:hydroxycinnamoyl transferase (HCT, AT5G48930) was suppressed |
display |
severe growth defects and sterility |
Arabidopsis thaliana |
| oligolignols and hexosylated oligolignols |
are severely reduced in |
ccr1-6 mutants compared to wild-type |
Arabidopsis thaliana |
| (ADT3, PD1, AT2G27820) /4/5 triple mutant |
displayed |
visual evidence of bolting stem structural weakening |
Arabidopsis thaliana |
| oligolignols accumulated in (AR2, ATR2, AT4G30210) mutant |
all had |
terminal H unit |
Arabidopsis thaliana |
| Arabidopsis reduced epidermal fluorescence8 (CYP98A3, REF8, AT2G40890) mutant |
display |
severe growth defects and sterility |
Arabidopsis thaliana |
| Caffeoyl-coenzyme A O-methyltransferase (CCoAOMT) |
is |
lignin biosynthesis gene |
|
| ccr1-6 mutants |
have |
lower S/G ratio |
Arabidopsis thaliana |
| OsERF71 overexpression |
elevates expression of |
lignin biosynthetic genes |
Oryza sativa |
| structural genes involved in lignin biosynthesis |
have been elucidated in |
model species |
Arabidopsis thaliana; Populus spp.; Medicago sativa |
| ATR2-deficient plants |
have |
6% less lignin |
Arabidopsis thaliana |
| regulatory process of OsFBK1-mediated OsCCR14 degradation |
has significant applications in |
wood-softening processes in wood and paper industries |
|
| feruloyl groups in glucuronoarabinoxylan |
are believed to act as nucleating sites for |
lignin formation |
|
| collapsed xylem vessels |
are similar to |
those found in CCR-down-regulated transgenic tobacco plants |
Nicotiana benthamiana; Nicotiana tabacum |
| down-regulation of NbCOMT |
is evidenced by |
almost unaltered secondary xylem structure |
Nicotiana benthamiana |
| Wiesner staining in (ATCAD4, CAD, CAD-C, CAD4, AT3G19450) c d mutant |
is increased but not to the same extent as in |
Wiesner staining in ccc mutant |
|
| ccc mutant |
displays |
red coloration of xylem vessels and fibers |
|
| (ATCAD4, CAD, CAD-C, CAD4, AT3G19450) silencing |
has negative impact on |
(ATGRP8, CCR1, GR-RBP8, GRP8, RBGA6, AT4G39260) expression |
|
| p-coumaryl alcohol, coniferyl alcohol, and sinapyl alcohol |
give rise to |
p-hydroxyphenyl (H), guaiacyl (G), and syringyl (S) lignin units |
|
| accumulation of p-coumarates, caffeates, and ferulates and their benzenoid derivatives in (AR2, ATR2, AT4G30210) mutants |
are |
logical consequence of reduced activity of (ATC4H, C4H, CYP73A5, REF3, AT2G30490) C3H1, and F5H1 |
Arabidopsis thaliana |
| (CYP98A3, REF8, AT2G40890) mutant |
deposits lignin largely derived from |
p-hydroxyphenyl subunits |
Arabidopsis thaliana |
| OsFBK1 knockdown line |
accumulates slightly more |
lignin |
Oryza sativa |
| (ATCAD7, CAD7, CHR, ELI3, ELI3-1, AT4G37980) |
has considerably lower activities against |
intermediates of the lignin biosynthetic pathway |
Arabidopsis thaliana |
| CCR-2 |
can partly compensate for |
(ATGRP8, CCR1, GR-RBP8, GRP8, RBGA6, AT4G39260) knockout phenotype |
Arabidopsis thaliana |
| CAD-silenced tobacco |
displays |
red coloration |
Nicotiana tabacum |
| toolbox (method plus imagej macro) |
will contribute to |
better understanding of how lignification is regulated at the cellular level |
|
| Plants silenced only for NbAsp5 |
showed |
normal phloroglucinol staining |
Nicotiana benthamiana |
| reducing lignin content in mutant or transgenic lines |
can result in |
reduced biomass yields |
|
| harpin-related protein |
is identified as candidate gene in |
QTL5 for lignin and 4-vinylphenol |
Zea mays |
| (ATGRP8, CCR1, GR-RBP8, GRP8, RBGA6, AT4G39260) -6 ProSNBE |
shows reduced S/G ratio at level even lower than |
ccr1-6 |
Arabidopsis thaliana |
| MYB proteins |
have been linked to affecting |
other components required for lignification |
|
| OsERF71 overexpression |
elevates |
lignification in rice roots |
Oryza sativa |
| cinnamyl alcohol dehydrogenase (ATCAD4, CAD, CAD-C, CAD4, AT3G19450) |
is considered |
key enzymatic step in monolignol biosynthesis |
|
| down-regulation of NbCOMT |
is evidenced by |
less lignin reduction in these plants |
Nicotiana benthamiana |
| moderate reduction of lignin in N. benthamiana plants |
does not significantly alter |
plant growth |
Nicotiana benthamiana |
| (ATCAD4, CAD, CAD-C, CAD4, AT3G19450) c d mutant |
displays two-fold lower |
(ATGRP8, CCR1, GR-RBP8, GRP8, RBGA6, AT4G39260) expression |
|
| original (CYP98A3, REF8, AT2G40890) mutant |
has |
missense mutation in C3'H |
Arabidopsis thaliana |
| lignin difference between ref8* and ref8* gir1-1 |
is not as clear in |
acetyl bromide assay |
Arabidopsis thaliana |
| enzymes in lignin biosynthesis |
contribute to |
different monomer composition and various ratios of guaiacyl or syringyl units |
|
| Arabidopsis (ATGRP8, CCR1, GR-RBP8, GRP8, RBGA6, AT4G39260) knockout mutants |
show |
reduced lignin formation |
Arabidopsis thaliana |
| specific translocation of monolignols by unknown (ATP binding cassette?) transporters |
is suggested for |
xylem formation in angio- and gymnosperms |
|
| CCR-down-regulated transgenic tobacco |
was |
severely stunted with spoon-like leaves |
Nicotiana tabacum |
| hydroxycinnamyl aldehyde |
is reduced to |
hydroxycinnamyl alcohol (monolignol) |
|
| angiosperm lignin |
contains abundant |
G and S units |
|
| laccase gene downregulation |
affects |
lignin content and spatial distribution |
Arabidopsis thaliana |
| this study |
has paid particular attention to |
routes leading to the biosynthesis of lignin |
|
| lignin alkali pretreatment |
could significantly increase |
endogenous lignin content in cassava |
Manihot esculenta |
| lignin biosynthesis perturbation |
results in |
severe growth and developmental defects |
|
| (CYP98A3, REF8, AT2G40890) mutant |
is |
dwarfed Arabidopsis lignin mutant |
Arabidopsis thaliana |
| COMT suppression |
apparently has |
fairly moderate or no effect on overall lignin content in tobacco |
Nicotiana tabacum |
| substantially increased acido-soluble lignin content |
might reflect |
important changes in lignin structure |
|
| safranin-O staining |
is used to evaluate and compare |
lignin contents in Arabidopsis thaliana lignin biosynthesis mutants |
Arabidopsis thaliana |
| (GMD2, MUR1, MUR_1, SFR8, AT3G51160) mutant |
showed |
lignification alterations |
|
| epistatic interactions of Rc and (ATIPT5, IPT5, AT5G19040) |
were found in |
targets of lignin metabolism |
|
| structural and regulatory genes |
lead to accumulation of |
gymnosperm and angiosperm lignin |
|
| (ATMYB58, MYB58, AT1G16490) and (ATMYB63, MYB63, AT1G79180) |
are direct activators of |
lignin biosynthetic genes |
Arabidopsis thaliana |
| phenylalanine ammonia lyase (PAL) encoding genes |
are regulated by |
conditions demanding increased lignin production |
|
| Klason lignin (KL) level |
is more reduced in |
ccc triple mutant |
|
| (ATCAD4, CAD, CAD-C, CAD4, AT3G19450) c d mutant |
displays reduced |
lignin content |
|
| (ATPAL1, PAL1, AT2G37040) (ATPAL2, PAL2, AT3G53260) (ATPAL3, PAL3, AT5G04230) (PAL4, AT3G10340) quadruple mutant |
shows |
defective lignin synthesis |
Arabidopsis thaliana |
| lignin content |
significantly increased with an increase in |
GhnsLTPsA10 expression |
Gossypium hirsutum; Arabidopsis thaliana |
| shikimate pathway |
contributes to |
lignin formation |
|
| down-regulation of NbCOMT |
has |
only moderate effects on lignin deposition |
Nicotiana benthamiana |
| (ATCRR2, CCR2, AT2G39180) and (CAD1, PROSCOOP5, AT5G44570) overexpression in ccc |
may result in lower remaining CCR and CAD activities than in transgenic tobaccos |
remaining CCR and (ATCAD4, CAD, CAD-C, CAD4, AT3G19450) activities |
Arabidopsis thaliana |
| (ATCAD4, CAD, CAD-C, CAD4, AT3G19450) D gene |
is expressed in |
reproductive tissues of WT samples |
Arabidopsis thaliana |
| cinnamyl alcohol dehydrogenase (ATCAD4, CAD, CAD-C, CAD4, AT3G19450) |
reduces |
hydroxycinnamyl aldehyde to hydroxycinnamyl alcohol (monolignol) |
|
| PbCCRSNL-like (accession number 103943280) |
transcript level is lower in |
peel of russet and semi-russet fruits and their rainfall-treated fruit compared to controls |
Pyrus × bretschneideri |
| qSH1 |
suppresses expression of by binding to promoter of |
4-COUMARATE: COENZYME A LIGASE 3 (4CL3, AT1G65060) |
|
| coniferyl alcohol |
is incorporated into lignin as |
guaiacyl (G) unit |
|
| this study |
infers |
evolutionary history of 11 critical enzyme families known to be woven into the mesh of routes from phenylpropanoids to lignin biosynthesis in land plants |
|
| cinnamoyl CoA reductase (CCR) and cinnamyl alcohol dehydrogenase (ATCAD4, CAD, CAD-C, CAD4, AT3G19450) |
are considered |
committed steps into monolignol, lignan, and lignin biosynthesis |
|
| simultaneous absence of CCR and (ATCAD4, CAD, CAD-C, CAD4, AT3G19450) activities |
has cumulative effect on |
content and composition of lignins of ccc mutant |
|
| CAD-silenced poplar |
displays |
red coloration |
Populus trichocarpa |
| SbCAD2 |
is related to |
internode phenotype |
sorghum |
| TaCAD1 |
is very similar to |
other bona fide CADs |
Triticum aestivum |
| temporal difference in gene expression of TaCCR1, TaCM, and TaCAD1 |
coincides with |
enzymes' positions in monolignol synthesis pathway |
Triticum aestivum |
| lignin formation |
may proceed via |
metabolic grid or precise channeling of individual precursors through metabolons |
|
| VIGS-NbCCR-infected plants |
are similar to |
CCR-down-regulated transgenic tobacco |
Nicotiana benthamiana; Nicotiana tabacum |
| genes |
regulate |
lignin biosynthesis and deposition at the cellular level |
|
| sinapoyl CoA |
is usually considered not to be |
direct precursor for lignin monomer production |
|
| down-regulation of NbCOMT |
has less severe impact on growth compared to |
down-regulation of Nb4CL and NbCCR |
Nicotiana benthamiana |
| gymnosperm lignin |
is typically composed of |
G units |
|
| ref8* gir1-1 suppressor plants |
are still deficient in |
G and S lignin |
Arabidopsis thaliana |
| transport of monolignols prior to assembly |
requires additional work on |
understanding mechanisms |
|
| acido-soluble lignin (ASL) |
is increased in |
ccc triple mutant |
|
| lignification |
is important for |
stem rigidity |
Arabidopsis thaliana |
| Wiesner staining |
is specific for |
coniferaldehyde end-groups in lignins |
|
| ccc mutant |
severely affects |
stem lignin structure |
Arabidopsis thaliana |
| (ATCAD4, CAD, CAD-C, CAD4, AT3G19450) c d mutant |
displays slightly increased lignin staining in |
stem cross-sections |
|
| reduction in lignin content through manipulation of monolignol biosynthetic pathway |
generally has |
undesirable pleiotropic effects |
|
| ferulic acid (FA) residues |
can be covalently linked to |
lignin monomers |
|
| genetic manipulation of lignin content |
may have potential to |
reduce recalcitrance of biomass |
Nicotiana benthamiana |
| absence of (ATCAD4, CAD, CAD-C, CAD4, AT3G19450) and CCR |
has more severe consequences on phenotype than |
individual absence of each of them |
Arabidopsis thaliana |
| CCR-silenced poplar |
displays |
orange-brown coloration |
Populus trichocarpa |
| cinnamoyl CoA reductase (CCR) |
converts |
hydroxycinnamoyl-CoA to hydroxycinnamyl aldehyde |
|
| derivatization followed by reductive cleavage (DFRC) method |
revealed |
lignin composition |
Arabidopsis thaliana |
| lignin deficiency |
leads to |
reduced biomass |
|
| mutations in med5b-3 and med2-3 |
restore growth phenotype without changing |
sinapoylmalate and lignin content in ref4-3 mutant |
Arabidopsis thaliana |
| (ATCAD5, CAD-5, CAD5, AT4G34230) (CINNAMYL ALCOHOL DEHYDROGENASE 5) |
did not show change in expression in |
SUB-OX leaves |
Nicotiana benthamiana |
| coniferin content in (UGT73C7, AT3G53160) knockout plants |
was slightly increased in |
(UGT73C7, AT3G53160) knockout plants after Pseudomonas syringae pv. tomato DC3000 treatment |
Arabidopsis thaliana |
| alternative biochemical pathway of syringyl–lignin biosynthesis |
occurs in |
higher plants |
|
| ccc mutant stem lignin |
shows incorporation into polymer of |
monolignol precursors |
Arabidopsis thaliana |
| (ATGRP8, CCR1, GR-RBP8, GRP8, RBGA6, AT4G39260) Arabidopsis mutant |
does not display |
orange-brown coloration |
Arabidopsis thaliana |
| marker compounds for (ATCAD4, CAD, CAD-C, CAD4, AT3G19450) and CCR deficiencies in ccc mutant |
demonstrates |
additive impact of the two missing lignin biosynthesis steps |
|
| (ATCAD4, CAD, CAD-C, CAD4, AT3G19450) c d mutant |
contains thioacidolysis marker compound for |
CCR deficiency |
|
| markedly altered lignin content and structure |
probably accounts for |
altered growth |
|
| sinapyl alcohol |
is incorporated into lignin as |
syringyl (S) unit |
|
| Overexpression of SUB |
did not cause induced expression of |
genes essential to lignin monomer synthesis |
Nicotiana benthamiana |
| cytochrome P450 enzyme that is orthologous to enzymes that act in lignin biosynthesis |
hinges on the action of |
enrichment of the Physcomitrium patens (moss) cuticle in phenolic compounds |
Physcomitrium patens |
| PbCCRSNL-6-like isoform X2 |
expression was severely suppressed in |
russet and semi-russet pears |
|
| PbCOMT2 |
transcript level was higher in |
russet ZS and semi-russet CG and in their rainfall-treated fruits compared to controls |
|
| (CCoAOMT1, AT4G34050) |
was originally considered to be only associated with |
monolignol formation |
|
| soluble lignin alkali pretreatment |
increased |
endogenous lignin content |
Manihot esculenta |
| brown-midrib maize |
shows |
improved digestion by ruminant animals |
Zea mays |
| reduced C3H1 activity |
is likely a direct result of |
10- to 15-fold increase in H units in (AR2, ATR2, AT4G30210) mutants |
Arabidopsis thaliana |
| epidermis cells |
do not |
lignify |
Arabidopsis thaliana |
| functional analysis of CB5s |
will shed light on |
putative role as electron donors of CYP450s involved in lignification |
Arabidopsis thaliana |
| NbAsp5 suppression in N. benthamiana |
resulted in |
no changes in lignin deposition |
Nicotiana benthamiana |
| suppression of (ATCAD4, CAD, CAD-C, CAD4, AT3G19450) and CCR |
may cause |
loosening of the lignin monomer |
|
| hydroxycinnamoyl CoA:shikimate/quinate hydroxycinnamoyltransferase (HCT, AT5G48930) |
initiates |
decisive step in lignin biosynthesis |
|
| drought |
promotes induction of |
(ATCAD4, CAD, CAD-C, CAD4, AT3G19450) expression and activity |
|
| lignin content in russet fruit peel |
increased sharply |
excess water application |
Pyrus pyrifolia Nakai |
| PbCOMT1 |
expression level was higher in |
rainfall-treated fruit |
|
| tricin |
is |
noncanonical lignin monomer |
|
| tricin |
is |
phytochemical |
|
| OsCAD2 |
is related to |
internode phenotype |
rice |
| Group I (ATCAD4, CAD, CAD-C, CAD4, AT3G19450) enzymes |
have been demonstrated to be involved in |
lignin biosynthesis |
Triticum aestivum |
| enzyme activity of TaCAD1 |
pointed to |
connection of TaCAD1 with stem lodging-resistance |
Triticum aestivum |
| CCR and COMT |
function in |
early stage of monolignol synthesis pathway |
Triticum aestivum |
| xylanase overexpression |
did not reduce |
lignin deposition in xylems |
|
| CAD-silenced Arabidopsis |
displays |
red coloration |
Arabidopsis thaliana |
| dramatically low yields of monomers from ccc mutant lignins |
suggests |
more than 95% of lignin units involved in resistant inter-unit bonds |
|
| cinnamyl alcohol dehydrogenase (ATCAD4, CAD, CAD-C, CAD4, AT3G19450) |
is |
rate-determining enzyme by which lignin is produced |
|
| less lignin level |
could lead to |
dead branches |
Populus |
| peroxidases (PODs) |
are |
key enzymes for the final step of lignin biosynthesis |
|
| genes for hydroxycinnamoyl-CoA transferase (E.C.2.3.1.133) |
downregulated in |
B+ Myc versus NoMyc |
Lotus japonicus |
| disruption of mediator |
rescues |
stunted growth of lignin-deficient Arabidopsis mutant |
Arabidopsis thaliana |
| conversion of feruloyl-CoA by cinnamoyl-CoA reductase (CCR), cinnamyl alcohol dehydrogenase (ATCAD4, CAD, CAD-C, CAD4, AT3G19450) and long chain acyl-CoA (LAC) |
creates |
lignin monomer |
|
| Ta-CCR1 and Ta-CCR2 recombinant enzymes |
could use |
feruloyl-CoA as substrate |
Triticum aestivum |
| transcript abundance of CCR |
is reduced by |
35% |
Picea abies |
| lower lignin content |
causes |
stems to be soft and brittle |
|
| cinnamoyl-CoA reductase (CCR) proteins |
convert |
CoA intermediates into their aldehydes |
|
| caffeoyl CoA-O-methyltransferase |
is involved in |
lignin biosynthesis |
Pyrus pyrifolia Nakai; Malus × domestica |
| cinnamoyl-CoA reductase |
is involved in |
lignin biosynthesis |
Pyrus pyrifolia Nakai; Malus × domestica |
| PbLAC |
expression level was higher in |
rainfall-treated fruit |
|
| lignin biosynthesis |
has main biosynthetic routes toward |
monolignols |
|
| (CYP98A3, REF8, AT2G40890) pOpON line |
can be |
conditionally complemented by chemically inducible expression of wild-type C3'H transgene |
Arabidopsis thaliana |
| peroxidase |
is involved in |
lignin biosynthesis |
Pyrus pyrifolia Nakai; Malus × domestica |
| MeRAV5 and MeCAD15 co-silencing |
results in |
lower lignin content |
Manihot esculenta |
| MeRAV5 |
interacts with |
lignin |
Manihot esculenta |
| (ATC4H, C4H, CYP73A5, REF3, AT2G30490) mutant |
has significantly reduced |
lignin content |
Arabidopsis thaliana |
| EjMYB2 transient overexpression alone |
failed to suppress |
lignin biosynthesis |
Nicotiana tabacum |
| genes coding for cinnamyl-alcohol dehydrogenase (EC:1.1.1.195) |
upregulated only in |
B− Myc versus NoMyc |
Lotus japonicus |
| downregulation of coniferin biosynthesis |
would increase accumulation of |
lignin precursors and lignin |
Arabidopsis thaliana |
| (ATCAD5, CAD-5, CAD5, AT4G34230) |
may be involved in |
lignin biosynthesis in response to salt stress |
Arabidopsis thaliana |
| PbCOMT1 (accession number 103927980) |
expression levels are significantly higher in |
peel of russet ZS fruit compared to non-russet CY fruit |
Pyrus × bretschneideri |
| SAMS2 |
is involved in |
lignin methylation |
|
| cinnamyl alcohol dehydrogenase |
is involved in |
lignin biosynthesis |
Pyrus pyrifolia Nakai; Malus × domestica |
| SvSH1 |
suppresses |
(ATECS1, AtGSH1, CAD2, GSH1, GSHA, PAD2, RAX1, RML1, AT4G23100) inflorescence expression |
Setaria viridis |
| regulation of KNOTTED ARABIDOPSIS THALIANA7 (TaKNAT7-4D) and PHENYLALANINE AMMONIA-LYASE (TaPAL1-6B) expression |
controls |
glume toughness |
|
| MeRAV5 |
is |
activator of (ATCAD4, CAD, CAD-C, CAD4, AT3G19450) activity |
Manihot esculenta |
| SEED SHATTERING 11 (OgSH11) |
suppresses expression of |
GOLD HULL AND INTERNODE2 (GH2) |
|
| functional genomics and genetic mapping |
has enabled |
decipher numerous possibilities of various metabolic routes towards lignin monomer and oligomer formation, oxidative phenol coupling, and analysis of polymeric structure |
poplar; pine; eucalyptus; alfalfa |
| dicot species |
contain |
two or three (ATCAD4, CAD, CAD-C, CAD4, AT3G19450) isoforms |
|
| TaCM gene expression and enzyme activity |
are higher in |
H4564 than in C6001 at heading and milky stages |
Triticum aestivum |
| monolignols |
is synthesized via |
shikimic acid pathway |
|
| suppression of CCR activity |
could lead to |
lignin monomer loosening and promote movement of phenolics such as ferulic and sinapic acids to the cell wall (CW) |
Pyrus × bretschneideri |
| lignin content |
was much higher in |
peel of russet pear and apple fruit compared to green non-russet fruit |
|
| Recombinant TaCAD1 protein |
showed high efficiencies toward |
p-coumaryl aldehyde |
Triticum aestivum |
| bm2 mutant |
shows reductions in |
guaiacyl (G)-residues |
Zea mays |
| BM1 |
function remains to be |
elucidated |
Zea mays |
| BM4 |
function remains to be |
elucidated |
Zea mays |
| induction of lignin biosynthesis pathway |
may be essential for |
accumulation of lignin along petal veins |
Brunfelsia |
| PbCAD1 |
contributes to |
biosynthesis of lignin monomers |
|
| tricin |
is |
noncanonical lignin monomer |
|
| Recombinant TaCAD1 protein |
used |
coniferyl aldehyde |
Triticum aestivum |
| high TaCAD1 gene expression |
would lead to |
enhanced lignin synthesis |
Triticum aestivum |
| composition of the surrounding matrix |
affects |
reaction kinetics |
|
| cinnamoyl-CoA reductase and dihydroflavonol 4-reductase |
are important for |
lignin biosynthesis |
Gossypium hirsutum |
| cellulase overexpression |
did not reduce |
lignin deposition in xylems |
|
| coniferyl alcohol |
undergoes polymerization to form |
monolignols |
|
| hydroxylation and methylation steps by cytochrome P450s and S-adenosyl-L-methionine (AdoMet)-dependent O-methyltransferases |
essentially determine |
contribution of guaiacyl and syringyl monomers to gymnosperm and angiosperm pattern |
|
| ccc mutant |
contains marker compounds for |
(ATCAD4, CAD, CAD-C, CAD4, AT3G19450) deficiency |
|
| (CYP98A3, REF8, AT2G40890) mutant |
exhibits |
LMID (lignin-related morphological inhibition of development) |
Arabidopsis thaliana |
| altered SUB expression |
accompanied by modified transcriptional programmes of |
lignin metabolism |
Arabidopsis thaliana |
| one lignin gene (LAC5, AT2G40370) |
was identified among |
21 genes with inverse expression |
Arabidopsis thaliana |
| SvSH1 |
binds promoter of |
(ATECS1, AtGSH1, CAD2, GSH1, GSHA, PAD2, RAX1, RML1, AT4G23100) |
Setaria viridis |
| secondary cell walls |
are cross-linked by |
phenyl propanoid-derived lignin meshwork |
|
| TaCAD1 |
is comparable to |
(ATCAD4, CAD, CAD-C, CAD4, AT3G19450) |
Triticum aestivum; Arabidopsis thaliana |
| TaCAD1 expression |
is critical at |
later stages of grain development |
Triticum aestivum |
| slight decrease in CCR expression |
does not alter |
pool of ferulic acid |
Zea mays |
| monolignols |
are polymerized to |
lignin |
|
| peroxidase |
converts phenolic compounds into |
lignin |
Arabidopsis thaliana |
| PbLAC |
transcript level was higher in |
russet ZS and semi-russet CG and in their rainfall-treated fruits compared to controls |
|
| change in lignin biosynthesis pathway |
is not caused directly by |
(UGT73C7, AT3G53160) overexpression |
Arabidopsis thaliana |
| plants |
can cope with large shifts in |
p-hydroxyphenyl/guaiacyl/syringyl (H/G/S) lignin compositional ratios |
|
| (ATMYB58, MYB58, AT1G16490) and (ATMYB63, MYB63, AT1G79180) |
redundantly regulate |
lignin biosynthetic genes including PHENYLALANINE AMMONIA LYASE 4 (PAL4, AT3G10340) |
|
| (ATMYB58, MYB58, AT1G16490) |
is regulated by |
VASCULAR-RELATED NAC-DOMAIN (VND) 6 and (ANAC030, VND7, AT1G71930) |
|
| lignin subunit composition |
varies between |
tissues within the same species |
|
| compounds derived from lignin |
are reduced in |
bm2, bm4, and bm2-bm4 mutants compared with wild type |
|
| midribs of bm2 and bm4 mutants |
show overall similar and statistically significant reductions in |
compounds derived from guaiacyl and syringyl residues in lignin |
|
| Recombinant TaCAD1 protein |
showed high efficiencies toward |
sinapyl aldehyde |
Triticum aestivum |
| one (ATCAD4, CAD, CAD-C, CAD4, AT3G19450) |
was showed to be responsible for |
lignin biosynthesis in sorghum |
Sorghum bicolor |
| BM2 |
function remains to be |
elucidated |
Zea mays |
| bm4 mutant |
causes |
11% reduction in Klason lignin content in midribs |
|
| SH5 |
directly suppresses expression of |
CINNAMYL-ALCOHOL DEHYDROGENASE 2 (ATECS1, AtGSH1, CAD2, GSH1, GSHA, PAD2, RAX1, RML1, AT4G23100) |
|
| cinnamyl-alcohol dehydrogenase (ATCAD4, CAD, CAD-C, CAD4, AT3G19450) |
is |
enzyme in lignin biosynthesis |
Brunfelsia |
| p-coumaryl alcohol |
gives rise to |
p-hydroxyphenyl (H) residues |
|
| (ATMYB63, MYB63, AT1G79180) |
is identified as |
activator of lignin biosynthesis |
Arabidopsis thaliana |
| EjMYB1 |
regulates |
lignin biosynthesis genes via binding to AC elements |
|
| (ATPAL1, PAL1, AT2G37040) (ATPAL2, PAL2, AT3G53260) double mutant |
shows significant reduction in |
lignin accumulation |
Arabidopsis thaliana |
| engineering of a single gene in the pathway |
is studied at |
organismal level |
|
| bsr-k1 |
binds to mRNAs of |
OsPAL1-7 |
|
| tricin |
has potential for |
enhancing lignin functionality |
|
| AtGA2ox overexpression |
decreased |
lignin content |
Nicotiana tabacum |
| (ATCAD1, CAD1, AT1G72680) gene |
partially restore |
(ATCAD4, CAD, CAD-C, CAD4, AT3G19450) (ATCAD5, CAD-5, CAD5, AT4G34230) double mutation |
Arabidopsis thaliana |
| Bm2 and Bm4 |
have different functions that are not part of |
linear pathway or process |
|
| OsCAD7 |
does not belong to |
bona fide (ATCAD4, CAD, CAD-C, CAD4, AT3G19450) family |
Oryza sativa |
| (ATCAD4, CAD, CAD-C, CAD4, AT3G19450) (ATCAD5, CAD-5, CAD5, AT4G34230) double mutation |
was necessary to reduce |
S lignin unit drastically |
Arabidopsis thaliana |
| functional (ATCAD4, CAD, CAD-C, CAD4, AT3G19450) |
exists as |
dimmer |
|
| catalytic and nucleotide-binding domains of (ATCAD4, CAD, CAD-C, CAD4, AT3G19450) |
are located inside |
cleft formed between two subunits of (ATCAD4, CAD, CAD-C, CAD4, AT3G19450) protein |
|
| down-regulation of cinnamoyl-CoA reductase (CCR) |
results in |
thinner and more irregular xylem walls |
tobacco |
| induction of lignin biosynthesis pathway |
may be essential for |
expansion of petals |
Brunfelsia |
| lignin subunit composition |
varies among |
different species |
|
| ectopic lignin in light-grown seedlings |
was not observed in |
wild-type (WT), the1-3, or prc1-1 |
Arabidopsis thaliana |
| tricin |
has potential for |
enhancing lignin functionality |
|
| flexible culm1 (fc1) mutant |
caused |
decrease in lignin content |
Oryza sativa |
| bm2-bm4 double mutant |
shows reductions in |
guaiacyl (G)-residues |
Zea mays |
| TaCAD1 |
is |
cinnamyl alcohol dehydrogenase from wheat |
Triticum aestivum |
| caffeoyl-CoA O-methyltransferase (CCoA-OMT) gene |
is putatively encoding |
one of three major enzymes in lignin biosynthesis pathway |
Brunfelsia |
| bm2 and bm4 mutations |
primarily affect |
leaves |
|
| gymnosperms |
contain |
one (ATCAD4, CAD, CAD-C, CAD4, AT3G19450) enzyme |
|
| two amino acid substitutions in TaCAD1 |
may be responsible for |
observed differences in substrate specificity |
Triticum aestivum |
| coniferyl alcohol |
gives rise to |
guaiacyl (G) units of lignin polymer |
|
| bm1 mutation |
is unlikely to be in |
(ATCAD4, CAD, CAD-C, CAD4, AT3G19450) gene |
Zea mays |
| bm2 mutant |
contains fewer |
guaiacyl residues |
Zea mays |
| brown colour in vascular tissue of bm mutants |
is suggested to be result of |
accumulation of aldehydes and/or ketones |
|
| down-regulation of lignin biosynthetic genes |
results in |
very thin secondary walls |
|
| PbCOMT2 (accession number 103951572) |
expression level is significantly higher in |
peel of semi-russet CG fruit compared to non-russet CY fruit |
Pyrus × bretschneideri |
| involvement of the lignin pathway upon mycorrhization |
already described in |
many plant models |
Solanum lycopersicum; Vitis vinifera; Medicago truncatula; Populus trichocarpa; Salix alba |
| (ATMYB63, MYB63, AT1G79180) |
is regulated by |
(ATMYB46, MYB46, AT5G12870) and (AtMYB83, MYB83, AT3G08500) |
|
| TaCAD1 |
is |
predominant (ATCAD4, CAD, CAD-C, CAD4, AT3G19450) in wheat stem |
Triticum aestivum |
| COMT, CCoA-OMT, and (ATCAD4, CAD, CAD-C, CAD4, AT3G19450) gene products |
increased in concentration during |
first 2 d after flower opening |
Brunfelsia |
| Zmccr1 mutant |
shows decrease in |
H lignin units |
Zea mays |
| wood |
is composed of |
lignin |
|
| multiple MYB regulation |
provides |
multiple levels of control for lignin biosynthesis |
|
| one (ATCAD4, CAD, CAD-C, CAD4, AT3G19450) |
was showed to be responsible for |
lignin biosynthesis in rice |
Oryza sativa |
| (ATCAD5, CAD-5, CAD5, AT4G34230) |
used |
sinapyl aldehyde with 64% activity of p-coumaryl aldehyde |
Arabidopsis thaliana |
| TaCAD1 |
used |
coniferyl aldehyde as most preferred substrate |
Triticum aestivum |
| NWYCY motif |
is essential for |
CCR activity |
Panicum virgatum |
| feruloyl-CoA |
is incorporated into |
lignins |
|
| MYB domain factors |
have previously been shown to affect |
monolignol biosynthesis |
|
| flexible culm1 (fc1) mutant |
caused |
decrease in (ATCAD4, CAD, CAD-C, CAD4, AT3G19450) activity |
Oryza sativa |
| CCR-deficient angiosperms |
revealed increased incorporation of free ferulic acid in lignins by |
bis-8-O-4 (cross) coupling |
Arabidopsis thaliana; Populus trichocarpa; Nicotiana tabacum |
| three (HCT, AT5G48930) two COMT, one CCR-encoding genes and one laccase-encoding gene |
down-regulated |
|
Zea mays |
| bm3 mutation |
occurs in |
gene encoding COMT |
Zea mays |
| (ATCCR2, CCR2, AT1G80820) and cinnamyl alcohol reductase |
leads to |
monolignol precursors of lignin |
Arabidopsis thaliana |
| our data |
pinpoint homologs for |
cinnamoyl-CoA reductase (CCR) |
|
| movement of phenolics such as ferulic and sinapic acids to the cell wall (CW) |
causes |
russeting in pear |
Pyrus × bretschneideri |
| OsCAD2 |
is related to |
hull phenotype |
rice |
| p-coumaryl alcohol |
gives rise to |
p-hydroxyphenyl (H) units of lignin polymer |
|
| apoplastic peroxidases |
use |
hydroxycinnamyl alcohols |
|
| catechol O-methyl transferase (COMT) |
is |
enzyme in lignin biosynthesis |
Brunfelsia |
| mutations in Bm3 gene |
result in incorporation of |
5-hydroxyconiferyl alcohol |
Zea mays |
| putative CCRs |
were expressed in |
lignifying ear internodes |
Zea mays |
| phenylpropanoid pathway |
results in |
monolignols |
|
| these results |
allow us to draw |
a comprehensive panorama of lignin synthesis modifications in plants under Pi starvation |
Zea mays |
| PtMYB8 overexpression |
causes significant increase in |
lignin content |
Picea glauca |
| MeRAV5 |
promotes activity of |
lignin-related cinnamyl alcohol dehydrogenase 15 (MeCAD15) |
Manihot esculenta |
| MeCAD15 silencing |
results in |
lower lignin content |
Manihot esculenta |
| slightly lower root lignin content in TR185 compared with WT |
was in agreement with |
repression of genes involved in lignin synthesis in TR185 |
Medicago truncatula |
| PEROXIDASE (POD) genes (LOC_Os06g16350 and LOC_Os07g48060) |
are related to |
lignin biosynthesis |
Oryza sativa |
| GOLD HULL AND INTERNODE2 (GH2) |
is |
key enzyme in the lignin biosynthetic pathway |
|
| G, S, and H monomers in Arabidopsis stem tissues |
accounted for |
69.8%, 28.3%, and 1.9% of total lignin respectively |
Arabidopsis thaliana |
| chemical control |
is proposed as mechanism for |
control of lignin subunit composition |
|
| down-regulation of cinnamyl alcohol dehydrogenase (ATCAD4, CAD, CAD-C, CAD4, AT3G19450) |
results in |
thinner and more irregular xylem walls |
tobacco |
| copper-dependent laccases |
control |
lignin deposition |
Cardamine hirsuta |
| wild q allele |
have |
more lignified and thicker glumes |
|
| (ATCAD5, CAD-5, CAD5, AT4G34230) |
used |
p-coumaryl aldehyde as most preferred substrate |
Arabidopsis thaliana |
| physiological role of (ATCAD4, CAD, CAD-C, CAD4, AT3G19450) in lodging-resistance |
has been confirmed in |
maize |
Zea mays |
| bm2-bm4 double mutant |
shows reductions in |
syringyl (S)-residues |
Zea mays |
| monolignols |
is synthesized via |
phenylpropanoid pathway |
|
| MADS-box transcription factor |
has role in |
lignification of Arabidopsis silique |
Arabidopsis thaliana |
| (ATGRP8, CCR1, GR-RBP8, GRP8, RBGA6, AT4G39260) and (HCT, AT5G48930) /C3H couple |
use |
coumaroyl-CoA as substrate |
|
| increased guaiacyl and syringyl monomers |
was reflected in |
increased lignin content |
Arabidopsis thaliana |
| TaCM |
is |
an authentic COMT involved in lignin biosynthesis |
Triticum aestivum |
| regulation of TaCM gene expression |
is involved in |
lignin synthesis |
Triticum aestivum |
| (ATGRP8, CCR1, GR-RBP8, GRP8, RBGA6, AT4G39260) |
plays a role in |
formation of minor H lignin units |
Zea mays |
| Arabidopsis plants overexpressing (IAA26, PAP1, AT3G16500) |
showed increased amounts of |
guaiacyl and syringyl monomers |
Arabidopsis thaliana |
| lignin O-methyltransferase (CCoAOMT, COMT) activities |
barely differed |
between wild-type and PAP1-overexpressing plants |
Arabidopsis thaliana |
| TaCAD1 mRNA abundance, protein level and enzyme activity in stem tissues |
were correlated to |
lignin contents of cultivars |
Triticum aestivum |
| TaCAD1 |
is |
pH-dependent enzyme |
Triticum aestivum |
| TaCCR1 (wheat cinnamoyl-CoA reductase 1) |
did not exhibit |
temperature sensitivity |
Triticum aestivum |
| EgMYB1 |
is reported as |
repressor of lignin biosynthesis |
Eucalyptus |
| discovery of additional monolignol transporters |
could represent important entry point for |
future manipulations of lignin biosynthesis pathway |
Arabidopsis thaliana |
| Mu insertion in the first intron of the CCR gene |
leads to |
slight decrease in CCR expression |
Zea mays |
| CCR down-regulation |
systematically reduces |
frequency of H lignin units |
|
| (ATGRP8, CCR1, GR-RBP8, GRP8, RBGA6, AT4G39260) |
exhibits higher affinity for |
feruloyl-CoA |
Oryza sativa |
| phenylalanine accumulation |
may arise under |
periods of low sink strength due to decreased activity of the phenylalanine ammonia-lyase |
|
| transgenic Norway spruce expressing the CCR gene in antisense orientation |
shows |
small decrease in lignin content |
Picea abies |
| CCR |
is |
cinnamoyl-CoA reductase |
|
| (ATGRP8, CCR1, GR-RBP8, GRP8, RBGA6, AT4G39260) and TaCM genes |
together regulate |
whole lignin biosynthesis pathway |
Triticum aestivum |
| lignin biosynthesis |
is important goal of |
switchgrass genomics research |
Panicum virgatum |
| expression of most of these 60 genes |
increased during |
maturation of different organs |
Panicum virgatum |
| lignin biosynthesis and deposition in wood |
is regulated by |
R2R3-MYBs |
|
| (ATCAD4, CAD, CAD-C, CAD4, AT3G19450) activity |
was reduced in |
bm1 mutant plants |
Zea mays |
| (ATCRR2, CCR2, AT2G39180) |
is |
highly up-regulated by thaxtomin A treatment |
Arabidopsis thaliana |
| lignin biosynthesis |
has been studied in |
model plants and woody trees |
|
| (AtMYB85, MYB85, AT4G22680) |
is identified as |
activator of lignin biosynthesis |
Arabidopsis thaliana |
| EjMYB1 |
is expected to be |
transcriptional activator |
Eriobotrya japonica |
| EjMYB2 |
clustered with |
EgMYB1 |
|
| EjMYB1 |
induces |
Ej4CL5 |
|
| CCR-deficient poplar, tobacco, and Arabidopsis |
contain higher amounts of |
G-CHSEt-CH2 (SEt)2 marker compound |
Populus trichocarpa; Nicotiana tabacum; Arabidopsis thaliana |
| H |
is |
p-hydroxyphenyl |
|
| VvMYB5a overexpression in transgenic tobacco |
led to |
reduced lignification in anther walls |
Nicotiana tabacum |
| PtMYB1 |
has been hypothesized to |
regulate lignin biosynthesis in differentiating xylem |
Pinus taeda |
| up-regulation of (4CL.1, 4CL1, AT4CL1, AT1G51680) (4CL2, AT4CL2, AT3G21240) and (ATC4H, C4H, CYP73A5, REF3, AT2G30490) |
supports |
induction of lignin biosynthesis at the molecular level |
Arabidopsis thaliana |
| transgenic Norway spruce displaying moderate CCR down-regulation |
shows significant decrease in |
minor H units |
Picea abies |
| Silicon (Si) nutrition |
increased |
lignin content |
Oryza sativa |
| an increase in the transcript level of the gene encoding CCoAOMT and of the laccase in proteoid roots of white lupin under Pi starvation |
reported |
|
Lupinus albus |
| different MYB genes |
may act differentially on |
genes within the lignin biosynthetic pathway |
Picea glauca |
| defence-related genes involved in lignin biosynthesis |
are the most sensitive to |
copper stress |
Oryza sativa |
| wheat TaCM gene expression |
plays role in |
lignin biosynthesis and stem development |
Triticum aestivum |
| lignins |
are involved in |
red colouration in woody stems |
|
| OsCAD2 |
is responsible for |
lignin biosynthesis |
rice |
| one (ATCAD4, CAD, CAD-C, CAD4, AT3G19450) |
is involved in |
lignin biosynthesis in monocot plants |
|
| TaCAD1 |
showed |
typical random reaction mechanism |
Triticum aestivum |
| TaCCR1 gene expression and enzyme activity |
are higher in |
H4564 than in C6001 at heading and milky stages |
Triticum aestivum |
| bm2 and bm4 mutations |
have limited effect on |
Klason lignin content in mature stems |
|
| baseline level of reactive oxygen species |
would allow |
incorporation of some H units |
|
| TaCM |
is |
caffeic acid 3-O-methyltransferase |
Triticum aestivum |
| ectopic lignification in thaxtomin A-treated seedlings |
is in agreement with |
altered expression of genes involved in the lignin biosynthetic pathway |
Arabidopsis thaliana |
| (ATCRR2, CCR2, AT2G39180) |
seems to have key role in |
thaxtomin A- and isoxaben-induced ectopic lignification |
Arabidopsis thaliana |
| Cinnamyl-alcohol dehydrogenase (FG471467) |
catalyses |
last step in monolignol synthesis |
|
| (ATMYB63, MYB63, AT1G79180) overexpression |
activates |
lignin biosynthesis genes |
Arabidopsis thaliana |
| E. grandiis EgMYB1 |
was shown to act as |
repressor of lignin biosynthesis |
Eucalyptus grandis |
| H2O2 |
is known to participate in |
lignin polymerization |
Nicotiana tabacum |
| Ta-CCR1 |
used |
feruloyl-CoA with greatest efficiency |
Triticum aestivum |
| TaCM gene expression |
is analyzed in relation to |
lodging-resistant phenotype in wheat |
Triticum aestivum |
| lignification |
occurs in |
fleshy fruit |
|
| (CYP98A3, REF8, AT2G40890) mutant |
has significantly reduced |
lignin content |
Arabidopsis thaliana |
| low-temperature conditioning (LTC) |
is effective in alleviating |
flesh lignification caused by chilling injury during long-term cold storage |
Eriobotrya japonica |
| (BEL1, AT5G41410) proteins |
may play role in |
lignin biosynthesis |
|
| (ATMYB58, MYB58, AT1G16490) overexpression |
activates |
lignin biosynthesis genes |
Arabidopsis thaliana |
| EjMYB genes |
particularly target |
Ej4CL genes |
|
| PgCCR-1 |
encodes |
cinnamoyl-CoA reductase |
Picea glauca |