| reversal potential of 8Br-cGMP-activated currents |
is far from |
equilibrium potential of Cl− |
Arabidopsis thaliana |
| animal CNGC subunits |
form |
heterotetrameric channels at plasma membrane |
|
| 8Br-cGMP-activated currents |
show |
Mg2+ permeability |
Arabidopsis thaliana |
| (ATCNGC5, CNGC5, AT5G57940) (ATCNGC6, CNGC6, AT2G23980) double mutation |
may have small effect on |
ABA-activated I Ca channel currents |
Arabidopsis thaliana |
| (ATCNGC5, CNGC5, AT5G57940) and (ATCNGC6, CNGC6, AT2G23980) |
are not essential for |
ABA-activated I Ca channels |
Arabidopsis thaliana |
| kinetics of [H+]cyt and [H+]vac during high KCl application |
indicated |
K+/H+ antiport dominates Cl−/H+ symport at both membranes |
|
| blockage of guard cell inward rectifying K+ currents by cytosolic Na+ |
has taken longer to develop than |
nearly instantaneous effect on intracellular Ca2+ seen here |
Arabidopsis thaliana |
| typical ocean surface seawater with pH ranges from 7.6 to 8.1 |
offers |
excellent sink to which phytoplankton may dump cytoplasmic protons |
phytoplankton |
| 8-Bromoguanosine 3′,5′-cyclic monophosphate (8Br-cGMP) |
activates |
inward currents |
Arabidopsis thaliana |
| reversal potential of 8Br-cGMP-activated currents |
is far from |
equilibrium potential of Cl− |
Arabidopsis thaliana |
| acetate (Ac) |
suppresses |
anion channels in guard cells |
Arabidopsis thaliana |
| ABA (abscisic acid) |
activates |
nonselective Ca2+-permeable cation channels |
Arabidopsis thaliana |
| AtSKOR |
is |
plant outward-rectifying K+ channel |
Arabidopsis thaliana |
| plasma membrane (PM) and tonoplast |
are connected in series |
two membrane systems |
|
| transient acidifications in mesophyll cells |
indicates |
initial prevailing anion/H+ influx into vacuole |
|
| Gd3+ |
inhibits |
8Br-cGMP-activated inward currents |
Arabidopsis thaliana |
| N-methyl-d-glucamine (NMDG) |
abolishes |
8Br-cGMP-activated inward currents |
Arabidopsis thaliana |
| interrelationships and functioning of membrane transport of K+, Cl−, Ca2+, and H+ |
paves way to |
holistic view of ion transport |
|
| (ALMT9, AtALMT9, AT3G18440) K93E/E130K |
mediated |
currents with time-dependent relaxations and a mean amplitude of −1.30 ± 0.27 nA at −120 mV |
Nicotiana benthamiana |
| K+ and Cl− ions |
are expelled through |
ion efflux channels |
|
| comparative RNA sequencing |
reveals enrichment of |
ion-transport functions among genes with higher expression in Schrenkiella parvula |
Schrenkiella parvula; Arabidopsis thaliana |
| stress-related ion transporter genes in Schrenkiella parvula |
showed increased basal expression strengths compared with |
Arabidopsis thaliana |
Schrenkiella parvula; Arabidopsis thaliana |
| Ser-899 phosphorylation |
is proposed to inhibit |
H+ -ATPase pump activity |
Arabidopsis thaliana |
| cold, H2O2, and mannitol treatments |
result in |
phosphorylation decreases |
Arabidopsis thaliana |
| lower net Na+ uptake into the shoot |
can be due to |
lower root–shoot transport of Na+ |
|
| Na+ efflux |
is |
energy-consuming process |
|
| MoMnr2 |
is essential for |
magnesium transport |
Magnaporthe oryzae |
| (ATCNGC2, CNGC2, DND1, AT5G15410) and (ATCNGC4, CNGC4, DND2, HLM1, AT5G54250) subunits |
may form |
heteromeric channels |
Arabidopsis thaliana |
| Arabidopsis thaliana |
contains |
over 150 cation transport proteins |
Arabidopsis thaliana |
| cngc5-1 cngc6-1 guard cells |
show measurable ABA activation of |
I Ca currents |
Arabidopsis thaliana |
| cpk6-1 guard cells |
do not show inhibition by yeast elicitor (YEL) of |
inward-rectifying K+ channels |
Arabidopsis thaliana |
| (ATCNGC5, CNGC5, AT5G57940) and (ATCNGC6, CNGC6, AT2G23980) |
contribute to |
activity of the I cat-cGMP channel currents |
Arabidopsis thaliana |
| cAMP |
activates |
Ca2+-permeable currents |
Arabidopsis thaliana |
| ABA-activated I Ca channels |
are permeable to |
Ba2+ |
Arabidopsis thaliana |
| chloride ion (Cl-) channels (ICl) in Arabidopsis guard cells |
show current relaxations and steady-state current-voltage relations typical of |
(ICL, AT3G21720) when stepped to voltages from +40 to −220 mV |
Arabidopsis thaliana |
| instantaneous nature of change in steady-state intracellular Ca2+ in response to NaCl in guard cell |
is suggestive of |
alteration in plasma membrane potential as consequence of increased external NaCl concentration |
Arabidopsis thaliana |
| aquaporins, K+ channels, Na+-H+ antiporters, cation-H+ antiporters, and sulfate transporters |
contained higher number of |
DEGPs in groups SpR and SpS |
Schrenkiella parvula |
| kinase-sensitive SLAC homologues |
are found in |
moss Sphagnum fallax |
Sphagnum fallax |
| repetitive high/low KCl (or KNO3) washings protocol |
was used to demonstrate |
strong coupling and synchronization of K+ and H+ transport at PM and VM |
|
| initial rapid decline in [H+]cyt |
was caused by |
K+-influx/H+-efflux across PM |
|
| W7 (CaM antagonist) |
can result in |
spontaneous CNGC current |
Arabidopsis thaliana |
| Ca2+-permeable channels in plant cells |
are permeable to |
Ca2+, Ba2+, and Mg2+ |
|
| 8Br-cGMP |
activates |
nonselective Ca2+-permeable cation currents |
Arabidopsis thaliana |
| 8Br-cGMP-activated currents |
can be carried by |
monovalent cation Na+ |
Arabidopsis thaliana |
| ABA-activated I Ca channels |
are permeable to |
Na+ |
Arabidopsis thaliana |
| (ABCB19, ATABCB19, ATMDR1, ATMDR11, ATPGP19, MDR1, MDR11, PGP19, AT3G28860) D1173N mutation |
abolished |
(ABCB19, ATABCB19, ATMDR1, ATMDR11, ATPGP19, MDR1, MDR11, PGP19, AT3G28860) function |
|
| antibody against the Arabidopsis vacuolar Na+/H+ antiporter (AT-NHX1, ATNHX, ATNHX1, NHX1, AT5G27150) |
did not detect |
protein in Thellungiella |
Thellungiella; Arabidopsis thaliana |
| suberin coating on endodermal cells in roots |
limits |
coupled transcellular pathways |
|
| pH value |
modifies |
root transmembrane proton gradient |
|
| K+ and anion channels of guard cells |
are |
pH-dependent |
|
| differences in anion/H+ antiport properties of CLCs |
could manifest in |
differences in nitrate vs chloride sequestration |
|
| cpk6-2 guard cells |
do not show inhibition by yeast elicitor (YEL) of |
inward-rectifying K+ channels |
Arabidopsis thaliana |
| Arabidopsis CNGC |
encode |
functional cyclic nucleotide-gated Ca2+-permeable channel |
Arabidopsis thaliana |
| 8Br-cGMP |
activates |
Ba2+ currents |
Arabidopsis thaliana |
| SlSKOR-mediated currents |
were carried mainly by |
K+ |
Xenopus laevis |
| 8Br-cGMP-activated inward currents |
result from |
influx of Mg2+ ions or efflux of Cl− |
Arabidopsis thaliana |
| I cat-cGMP |
was reduced in |
cngc5-1 mutant guard cells |
Arabidopsis thaliana |
| a single amino acid mutation |
converts |
an outward K+ channel (SKOR, AT3G02850) into an inward K+ channel |
|
| SLAH channels in angiosperms |
function in |
nutrient movement |
|
| vacuolar membrane (VM) |
contains |
K+/H+ symporters |
|
| Ca2+ channels (ICa) in intact guard cells |
show reversal voltage close to |
expected equilibrium voltage for Ba2+ |
Arabidopsis thaliana |
| CsMATE1 |
displayed no activity toward |
chloride |
Xenopus laevis |
| CmMATE1 |
displayed no activity toward |
chloride |
|
| SLAC homologues |
may have role in |
nutrient movement in bryophytes |
|
| general K+/H+-antiport feature at PM and VM |
is |
common feature |
|
| potassium (K+) transport at PM and VM |
points toward |
efficient K+/H+ antiport mechanism at both membranes |
|
| lanthanum ion (La3+) |
blocks |
Ca2+ channels (ICa) |
Arabidopsis thaliana |
| cngc5-1 cngc6-1 double mutant guard cells |
show strongly impaired |
8Br-cGMP-activated currents |
Arabidopsis thaliana |
| vacuolar membrane (VM) |
contains |
cation/H+ antiporters |
|
| inward-rectifying K+ channels (IK,in) in Arabidopsis guard cells |
show current relaxations with |
half-times of 400 to 600 ms |
Arabidopsis thaliana; Vicia; Nicotiana tabacum |
| (ATCNGC1, CNGC1, AT5G53130) (ATCNGC20, CNBT1, CNGC20, AT3G17700) double mutant |
show no significant difference in |
I cat-cGMP |
Arabidopsis thaliana |
| ABA-activated I Ca channels |
are permeable to |
Mg2+ |
Arabidopsis thaliana |
| CaM binding |
reduces |
CNGC-dependent Ca2+ influx into cells |
Arabidopsis thaliana |
| cyclic nucleotide-activated ion channels |
function as |
cyclic nucleotide-gated Ca2+-permeable channel |
|
| 8Br-cGMP-activated Ba2+ currents |
show |
activation is not time dependent |
Arabidopsis thaliana |
| increased salt concentrations |
inhibit |
potassium accumulation in guard cells |
|
| CaM binding |
diminishes |
cyclic nucleotide-dependent activation of CNGCs |
Arabidopsis thaliana |
| (ATCNGC2, CNGC2, DND1, AT5G15410) mutant guard cells |
show no significant difference in |
I cat-cGMP |
Arabidopsis thaliana |
| ABA-insensitive mutants growth controlled by abscisic acid2 (ATCPK23, CPK23, GCA2, AT4G04740) and abscisic acid insensitive1 (abi1-1) |
show impaired |
ABA activation of I Ca channel currents |
Arabidopsis thaliana |
| (AtBRC1, ATTCP18, BRC1, TCP18, AT3G18550) directly regulates |
genes encoding transmembrane proteins involved in |
inter- and intracellular transport of ions |
Arabidopsis thaliana |
| cpk6-1 guard cells |
do not show activation by yeast elicitor (YEL) of |
slow anion channels |
Arabidopsis thaliana |
| (AHA8, HA8, AT3G42640) |
shows lower expression in |
ced2 mutant |
Arabidopsis thaliana |
| K+ channels, cation/H+ antiporters, K+/H+ symporters, anion/H+ exchangers, and anion channels |
contribute to |
ion exchange between vacuole and cytosol |
|
| Prior exposure to W7 |
increased |
PAMP (LPS)-dependent inward current through Ca2+-conducting channels |
Arabidopsis thaliana |
| (ATCNGC2, CNGC2, DND1, AT5G15410) |
can form |
ion channels that conduct both Ca2+ and K+ |
|
| Arabidopsis genome |
includes |
20 genes encoding homologs to animal Glu receptor channels |
Arabidopsis thaliana |
| 8Br-cGMP-activated currents |
show spiky nature similar to |
Ca2+-permeable cation channel currents |
Arabidopsis thaliana |
| permeability ratio for Ba2+ relative to Ca2+ |
was determined according to |
Goldman-Hodgkin-Katz equation |
|
| (AHA1, HA1, OST2, PMA, AT2G18960) open stomata2-2D ( -2D) mutant plants |
have |
constitutive higher PM H+-ATPase activity |
Arabidopsis thaliana |
| nonselective cation channels (NSCCs) |
mediate |
Na+ influx |
|
| kinase-sensitive SLAC homologues |
are found in |
hornwort Anthoceros agrestis |
Anthoceros agrestis |
| two-electrode voltage clamp |
is used to record |
inward-rectifying K+ channels (IK,in) and chloride ion (Cl-) channels (ICl) currents during abscisic acid (ABA) treatments |
Arabidopsis thaliana |
| (ANN1, ANNAT1, AtANN1, ATOXY5, OXY5, AT1G35720) |
is responsible for |
root epidermal plasma membrane Ca2+- and K+-permeable conductance |
Arabidopsis thaliana |
| SpNHX8;3 |
showed significantly higher expression in |
S. parvula roots |
Schrenkiella parvula |
| CATION/PROTON EXCHANGER21 (ATCHX21, CHX21, AT2G31910) |
encodes |
K+ transporters |
Arabidopsis thaliana |
| CATION/PROTON EXCHANGER23 (ATCHX23, CHX23, AT1G05580) |
encodes |
K+ transporters |
Arabidopsis thaliana |
| lower net Na+ uptake into the shoot |
can be due to |
higher Na+ recycling from the shoots into the roots |
|
| (PHT4;6, AT5G44370) |
does not facilitate transport of |
inorganic anions |
Arabidopsis thaliana |
| NaPi-1 protein from rabbit kidney cells |
is permeant to |
chloride and organic anions |
|
| asymmetry in H+-ATPase manipulation effects |
is the direct consequence of |
nonlinearity in pump capacity |
Arabidopsis thaliana |
| proton pump |
has |
proton reversal potential much larger than sodium/potassium pump |
|
| root surface alkalization |
leads to downstream regulation of |
plasma membrane (PM)-H + -ATPase |
|
| KEA transporters |
require determination of |
transport mode |
|
| Cs+ and TEA+ |
do not inhibit |
Na+ influx |
Thellungiella; Arabidopsis thaliana |
| ARABIDOPSIS VACUOLAR H+-PYROPHOSPHATASE1 (AtAVP1, ATAVP3, AtVHP1;1, AVP-3, AVP1, FUGU5, VHP1, AT1G15690) |
homologs showed increased copy numbers resulting in |
overall higher expression strengths with expression observed for all duplicates |
Schrenkiella parvula |
| salt-tolerant species Salicornia parvula and Eutrema salsugineum |
show higher basal-level expression of |
stress-related ion transporters |
Salicornia parvula; Eutrema salsugineum |
| SpNHX8;1 and SpNHX8;3 |
are significantly higher in basal-level expression compared with |
(ATNHX8, NHX8, AT1G14660) in shoot and root tissues, respectively |
Salicornia parvula; Arabidopsis thaliana |
| nitrate transporters, metal transporters, and P-type pump gene families |
showed more |
DEGPs in groups (ATATR, ATR, ATR-2, ATRAD3, AT5G40820) and (ATS, KAN4, AT5G42630) |
Arabidopsis thaliana |
| IAA − (indole-3-acetic acid anion) concentration gradient |
did not result in measurable shift in |
reversal voltage of the I-V curve |
|
| (ATCCH1, ATTPC1, FOU2, TPC1, AT4G03560) /SV channel |
relates to |
vacuolar physiology |
|
| K+ EFFLUX ANTIPORTER1 (ATKEA1, KEA1, AT1G01790) |
homologs showed increased copy numbers resulting in |
overall higher expression strengths with expression observed for all duplicates |
Schrenkiella parvula |
| (ATCIPK14, ATSR1, CIPK14, PKS24, SnRK3.15, SR1, AT5G01820) mutants |
show |
the highest H+-transport activity |
Arabidopsis thaliana |
| (ATSAC1, SAC1, AT1G22620) mutant |
encodes protein that shares similarity with |
ion transporters |
Chlamydomonas reinhardtii |
| HEK cells expressing (ABCB19, ATABCB19, ATMDR1, ATMDR11, ATPGP19, MDR1, MDR11, PGP19, AT3G28860) |
display |
substantially greater ionic currents |
|
| unidirectional Na+ influx into the roots |
is significantly smaller in |
Arabidopsis |
Thellungiella; Arabidopsis thaliana |
| mutated forms of (AtKAT1, KAT1, AT5G46240) |
reduced the activity of |
wild-type guard cell channel function |
Arabidopsis thaliana |
| (AHA1, HA1, OST2, PMA, AT2G18960) pT948 and (AHA2, AtHA2, HA2, PMA2, AT4G30190) pT947 |
are responsive to |
several treatments |
Arabidopsis thaliana |
| higher selectivity of VIC for K+ over Na+ |
limits |
Na+ influx into root cells |
Thellungiella |
| PHT4;6 protein |
was initially annotated to be |
putative anion/cation co-transporter |
Arabidopsis thaliana |
| PM-H+-ATPase |
actively pumps protons out of |
cell |
|
| (PHT4;6, AT5G44370) |
could transport |
inorganic anions |
|
| nuclear pore complex (NPC) |
has |
ion conductivity |
|
| iris-like structures proposed for the central channel |
differ from |
iris-like structures suggested for the nuclear basket |
|
| advanced electrophysiological tools and optical probes |
need to be carried out on |
nuclei from multiple cell types grown under various conditions |
|
| V-type ATPase |
is located in |
vacuolar membrane |
|
| RALF-LIKE 36 (RALFL36) in presence of lanthanum (La3+) |
causes |
small increase in H+ efflux |
Arabidopsis thaliana |
| SpNHX8;1 |
showed significantly higher expression in |
S. parvula shoots |
Schrenkiella parvula |
| homologs encoding two potassium transporters, (ATKEA1, KEA1, AT1G01790) and (ATKUP9, HAK9, KT9, KUP9, AT4G19960) |
are duplicated in |
Salicornia parvula and show higher basal-level expression in roots |
Salicornia parvula |
| transgenic plants harboring (ATCIPK14, ATSR1, CIPK14, PKS24, SnRK3.15, SR1, AT5G01820) |
show H+-transport activity that is |
the same as in wild-type |
Arabidopsis thaliana |
| (ATNHX7, ATSOS1, SOS1, AT2G01980) |
exports Na+ from |
xylem |
Thellungiella |
| DEGP groups (ATATR, ATR, ATR-2, ATRAD3, AT5G40820) and (ATS, KAN4, AT5G42630) |
were enriched for |
nitrate transport and metal ion transport processes |
Arabidopsis thaliana |
| SpAVP1;1 and (AtAVP1, ATAVP3, AtVHP1;1, AVP-3, AVP1, FUGU5, VHP1, AT1G15690) |
are strongly expressed with mean normalized RNA-seq read count higher than |
99.5% of the entire S. parvula and Arabidopsis genes |
Schrenkiella parvula; Arabidopsis thaliana |
| (ATCIPK14, ATSR1, CIPK14, PKS24, SnRK3.15, SR1, AT5G01820) protein |
has no significant effect on |
H+-transport activity in wild-type vesicles |
Arabidopsis thaliana |
| (AHA10, TT13, AT1G17260) |
shows lower expression in |
ced2 mutant |
Arabidopsis thaliana |
| (ATNHX7, ATSOS1, SOS1, AT2G01980) export of Na+ |
limits |
Na+ transport into the shoot |
Thellungiella |
| SpHKT1;1 |
was expressed specifically in |
shoots |
Schrenkiella parvula |
| NPA (1-naphthylphthalamic acid) |
did not affect |
(ABCB19, ATABCB19, ATMDR1, ATMDR11, ATPGP19, MDR1, MDR11, PGP19, AT3G28860) ion channel activity |
|
| H+-ATPASE4 ( (AHA4, HA4, AT3G47950) ) |
shows lower expression in |
ced2 mutant |
Arabidopsis thaliana |
| abscisic acid (ABA) |
inhibits |
plasma membrane H+-ATPase (PM H+-ATPase) activity |
|
| increasing the Kout population |
anticipated benefit is offset by |
change in free-running voltage as K+ efflux adjusts with that of the anions |
Arabidopsis thaliana |
| (PHT4;6, AT5G44370) |
does not facilitate transport of |
chloride |
Arabidopsis thaliana |
| (ANN1, ANNAT1, AtANN1, ATOXY5, OXY5, AT1G35720) |
does not contribute to |
root Na+ uptake |
Arabidopsis thaliana |
| guard cells |
regulate fluxes of |
K+, Cl−, and malate |
|
| high concentrations of sodium ions in soil |
inhibits |
potassium uptake |
|
| homologs of Na+/H+ EXCHANGER1 (AT-NHX1, ATNHX, ATNHX1, NHX1, AT5G27150) and (ATNHX2, NHX2, AT3G05030) and ARABIDOPSIS K+ TRANSPORTER1 |
do not show differences in |
copy number or basal-level expression in Salicornia parvula |
Salicornia parvula |
| NPPB (5-nitro-2-(3-phenylpropylamino)-benzoic acid) |
is established as a blocker of |
(ABCB19, ATABCB19, ATMDR1, ATMDR11, ATPGP19, MDR1, MDR11, PGP19, AT3G28860) activity |
|
| SpKEA1;2 (Sp1g00500) |
had similar expression strengths with |
(ATKEA1, KEA1, AT1G01790) |
Schrenkiella parvula; Arabidopsis thaliana |
| HCO3− (or any anion) |
would have to move against |
electrical gradient to enter chloroplast |
Chlamydomonas reinhardtii |
| voltage-independent channel (VIC) |
genes encoding have not yet been |
identified |
|
| decreased Na+ permeability |
is related to |
energy conservation |
|
| voltage-independent channel (VIC) |
has higher selectivity for K+ over Na+ than |
respective system in Arabidopsis |
Thellungiella; Arabidopsis thaliana |
| membrane potential of chloroplast envelope |
is |
inside negative |
Chlamydomonas reinhardtii |
| (AHA1, HA1, OST2, PMA, AT2G18960) and (AHA2, AtHA2, HA2, PMA2, AT4G30190) |
display |
coordinated regulation at penultimate position |
Arabidopsis thaliana |
| proton influx |
occurs in root tips, peaking in |
root transition zone |
Pisum sativum |
| low similarity of antigenicity in NHX-type transporters between the two species |
may explain |
lack of protein detection in Thellungiella |
Thellungiella; Arabidopsis thaliana |
| (PHT4;6, AT5G44370) |
shows sequence similarity to |
chloride transport proteins |
|
| electrophysiological methods |
challenge |
conventional view of NPC ion permeability |
|
| R-type anion channel |
corresponds to |
aluminum-activated malate transport (ALMT12, ATALMT12, QUAC1, AT4G17970) |
|
| SV channels |
permeate |
potassium (K+) |
Arabidopsis thaliana; Hordeum vulgare; Beta vulgaris; Plantago maritima; Allium cepa |
| SV channels from Plantago maritima |
show almost equal permeability for |
potassium and sodium |
Plantago maritima |
| citrate |
is suggested to competitively inhibit |
malate uptake across the tonoplast |
|
| absence of K+ in nigericin-free medium |
prevents |
pH shifts |
|
| (CDI3, OZS1, RCD3, SLAC1, AT1G12480) mutation |
does not affect |
rapid (R)-type anion currents |
|
| V-ATPase |
transports |
cations |
|
| ost2-2D mutant |
is mutation in |
Arabidopsis PM H+-ATPase (AHA1, HA1, OST2, PMA, AT2G18960) |
Arabidopsis thaliana |
| negative membrane potential |
activates |
K+ inward rectifying channels |
Thellungiella |
| (AtAVP1, ATAVP3, AtVHP1;1, AVP-3, AVP1, FUGU5, VHP1, AT1G15690) |
showed higher sequence similarity with |
SpAVP1;1 |
Arabidopsis thaliana; Schrenkiella parvula |
| (AHA2, AtHA2, HA2, PMA2, AT4G30190) down-regulation mechanisms |
vary based on |
treatment conditions |
Arabidopsis thaliana |
| difference in Na+ inward current between Thellungiella and Arabidopsis |
could be attributed to |
higher selectivity of the Thellungiella VIC for K+ over Na+ |
Thellungiella; Arabidopsis thaliana |
| nuclear patch clamping experiments |
supported |
idea that NPCs possess ion channel activity |
|
| (ATCCH1, ATTPC1, FOU2, TPC1, AT4G03560) gene product |
prevents |
potassium ion release into the cytosol |
Arabidopsis thaliana |
| cells expressing EGFP exposed to 0 or 150 mM NO3− solution |
show little divergence of |
electrode voltage–current curves |
Homo sapiens |
| modulation of membrane potential |
controls |
ion flux at PM |
|
| SV channel |
has |
selectivity |
|
| SV channel |
has |
interactions with different ionic species |
|
| amino acid residue(s) in the (ATCCH1, ATTPC1, FOU2, TPC1, AT4G03560) gene product |
determine |
SV channel voltage dependence |
|
| patch clamping of endoplasmic reticulum (ER) membranes from red beet taproots |
detected |
somewhat similar channel |
Beta vulgaris |
| Na+/H+-antiporters at the tonoplast |
cause |
efflux of vacuolar protons |
Eschscholzia californica |
| 20 mM K-HEPES, pH 7.5, containing 40 mM KCl |
stops |
K+ efflux |
|
| plasma membranes of higher plant cells |
are equipped with |
ion channels |
|
| Ca2+ |
promotes |
ion efflux |
|
| Na+ competition for transporter sites |
has been reported for |
soybean |
soybean |
| macronutrient Na |
exists primarily in plant tissues in |
ionic form |
okra |
| neutralization of autoinhibition of the pump |
activates |
proton pumping out of the cell |
|
| fer-4 seedlings treated with RALF-LIKE 36 (RALFL36) |
lack |
flux reversal observed for (RALF33, RALFL33, AT4G15800) |
Arabidopsis thaliana |
| members of the ALMT family |
present |
significant permeability for more than one anion |
|
| import of sugars |
might affect |
membrane potential of yeast cells |
Saccharomyces cerevisiae |
| proton pumps |
generate |
pH gradients of 1–2 pH units |
|
| electrochemical driving forces |
determine direction of |
potassium ion transport |
Arabidopsis thaliana |
| nuclear envelopes of amphibian oocytes |
have |
exceptionally low electrical resistance |
Amphibia |
| plasma membrane ATPase |
is encoded by |
transcript showing similar changes in u-ATP9 and rotenone-treated cells |
Arabidopsis thaliana |
| SV channel in Vicia faba guard cells |
transports |
Na+ |
Vicia faba |
| vacuolar sodium loading at threshold concentration of about 50 mM |
prevents |
potassium ion release into the cytosol |
Arabidopsis thaliana |
| Fumonisin B1 (FB1) |
inhibits |
other P-type ATPases |
|
| central channel and peripheral channels of NPC |
regulate |
ion flow through the NPC |
|
| P-type ATPase |
is located in |
plasma membrane |
|
| single TPC2 channels |
would have |
very small unitary Ca2+ current |
Homo sapiens |
| Pseudomonas lipopeptide toxins |
can inhibit in a synergistic manner |
PM H+-ATPase enzyme activity |
|
| PHOSPHATE DEFICIENCY RESPONSE 2 (MIA, PDR2, AT5G23630) |
encodes |
P5-type ATPase |
Arabidopsis thaliana |
| genes with loss of regulation in coi1-16 during K re-supply |
include |
cation co-transporter (CAX7, CCX1, AT5G17860) |
Arabidopsis thaliana |
| allelic variation |
can affect |
transport rates |
|
| SV from B. vulgaris |
has Ca2+ conductance of |
13 pS |
Beta vulgaris |
| active Na+ efflux |
is presumed to be |
mechanism in non-vacuolated root meristem cells |
|
| depolarisation of the plasma membrane |
results in |
efflux of potassium (K+) |
Pisum sativum; Zea mays; Arabidopsis thaliana |
| (ATCCH1, ATTPC1, FOU2, TPC1, AT4G03560) gene |
encodes |
SV channel |
Arabidopsis thaliana |
| kinetics of tracer uptake assay |
reflected the difference between |
(ATMGT5, MGT5, MRS2-6, AT4G28580) and (ATMGT10, GMN10, MGT10, MRS2-11, AT5G22830) (ATMGT1, MGT1, MRS2-10, AT1G80900) transport properties |
Salmonella |
| K+ removal from growth medium for 7 h |
prompted marked inhibition of |
K+ flux in the xylem sap (JK) |
|
| Al tolerance in the alr104 mutant |
correlates with |
plasma membrane depolarization (Em) |
Arabidopsis thaliana |
| export of Na+ by an Na+/H+ antiporter |
will change |
cytosolic and vacuolar pH |
|
| LATS |
shows |
linear responses to substrate concentrations |
|
| plant AMTs |
have been shown to perform |
electrogenic transport |
|
| Aluminum ion (Al 3+) |
inhibits |
Magnesium ion (Mg 2+) -permeable cation channels |
|
| SLOW ANION CHANNEL-ASSOCIATED 1 (CDI3, OZS1, RCD3, SLAC1, AT1G12480) homotrimer |
contains |
central five-helix transmembrane pore |
|
| proton motive force (pmf) |
is used to |
accumulate calcium, sodium, and potassium ions in the vacuolar lumen |
|
| (RALF33, RALFL33, AT4G15800) and RALF-LIKE 36 (RALFL36) treatment |
shifts H+ flux towards |
net uptake |
Arabidopsis thaliana |
| H+ response shift in fer-4 seedlings treated with (RALF33, RALFL33, AT4G15800) |
indicates |
proton pump had not been inactivated by (RALF33, RALFL33, AT4G15800) treatment |
Arabidopsis thaliana |
| NPCs |
are, at most, a diffusion barrier for |
Ca2+ |
|
| (CDI3, OZS1, RCD3, SLAC1, AT1G12480) mutant |
is diminished in |
anion current (NO3−, Cl−) |
|
| vacuolar membrane |
contains |
ion channels |
|
| cation channels in suspension-cultured cells |
can transport |
sodium ions into the vacuole |
Arabidopsis thaliana |
| (ATCCH1, ATTPC1, FOU2, TPC1, AT4G03560) gene identification |
opened perspective for |
genetic manipulations to explore SV channel function |
Arabidopsis thaliana |
| reduction in membrane potential |
can cause deleterious effects on |
other transporters driven by the membrane potential |
Arabidopsis thaliana |
| patch clamp detection of ion channels in nuclei isolated from immature coconut endosperm |
minimized |
possibility that nucleus-associated ion channel activity was due to contamination with other cellular membranes |
Cocos nucifera |
| exogenous spermidine application |
significantly blocks |
inward Na+ currents in epidermal and cortical cells of barley roots |
Hordeum vulgare |
| SV channel |
is encoded by |
(ATCCH1, ATTPC1, FOU2, TPC1, AT4G03560) gene |
|
| (ATEDS1, EDS1, AT3G48090) – helper NLR nuclear complexes |
may form channels at |
nuclear membrane |
|
| modulation of membrane potential |
changes activities of |
voltage-gated channels |
|
| open SV channels |
have Na+ permeability |
Na+ permeability |
Arabidopsis thaliana |
| SV channels |
is product of |
(ATCCH1, ATTPC1, FOU2, TPC1, AT4G03560) |
Arabidopsis thaliana |
| SbMATE |
shows high affinity for |
Na+ and H+ |
Sorghum bicolor |
| PEP1-responsive DEGs in WT plants |
are significantly enriched for functions including |
ion transport |
Oryza sativa |
| findings on independent regulation of Ca2+ in the nuclear compartment |
gained further support for |
possibility of ion impermeable states of the NPC |
|
| NPCs |
can be gated for |
Ca2+ |
|
| NIP3 |
activates |
plasma membrane H+ ATPase (PM H+-ATPase) |
Hordeum vulgare |
| Pseudomonas lipopeptide toxins |
can inhibit |
PM H+-ATPase enzyme activity |
|
| genomes of higher plants, ferns, and mosses |
do not harbor |
animal-type sodium or calcium channel genes |
|
| SV channels |
do not mediate |
Na+ release from the vacuole |
Arabidopsis thaliana |
| Group-3 Shaker channels |
display |
weak rectification |
Arabidopsis thaliana |
| SODIUM HYDROGEN EXCHANGER4 (ATNHX4, NHX4, AT3G06370) |
encodes |
Na+/K+ antiporter |
|
| ion transporters |
confer |
salt stress tolerance in rice |
Oryza sativa |
| lower Na+ content of Thellungiella |
is explained by |
findings regarding membrane potential and ion transport |
Thellungiella |
| peripheral channels of NPC |
allow |
constitutive ion flow |
|
| cation-selective, Ca2+- and voltage-dependent channel |
is located in |
nuclear envelope (NE) of red beet |
Beta vulgaris |
| SV channel |
is |
slow-activating vacuolar channel |
|
| SV channels |
permeate |
calcium (Ca2+) |
Arabidopsis thaliana; Hordeum vulgare; Beta vulgaris; Plantago maritima; Allium cepa |
| AtMGT5-transformed cells |
began to show inhibition of tracer uptake when |
Mg2+ concentration reached 0.1 mM |
Salmonella |
| efflux rate of (ATMGT5, MGT5, MRS2-6, AT4G28580) cells |
was much higher than |
MM281 or (ATMGT10, GMN10, MGT10, MRS2-11, AT5G22830) cells |
Salmonella |
| (ATMGT5, MGT5, MRS2-6, AT4G28580) |
was not sensitive to |
aluminum (Al) |
Salmonella |
| passive diffusion of ammonia (NH3) |
could be alternative explanation for |
active, energy-consuming extrusion mechanism |
|
| present data |
indicate that the entry of Na+ into shoots under these conditions is reduced by almost an order of magnitude |
Na+ entry into shoots |
Oryza sativa |
| further investigations using advanced electrophysiological tools and optical probes |
are needed to judge |
full ion conductance characteristics of NPCs |
|
| thylakoid membrane of chloroplasts |
contains |
ion channels |
|
| amino acid residue(s) in the (ATCCH1, ATTPC1, FOU2, TPC1, AT4G03560) gene product |
determine |
SV channel conductance |
|
| limited K+ uptake into Vicia faba guard cell protoplasts |
observed when treated with |
red light with K+ present in solution |
Vicia faba |
| OsPIP1;3 |
detected significant |
NO3− conductance |
Mammalia |
| other ion transporters |
serve as |
shunt conductances |
|
| low-affinity HKT transporters in xylem parenchyma cells |
retrieve Na+ from |
xylem sap |
|
| 24 Na+ tracer analysis |
is used to determine |
translocation of Na+ to the shoot |
Oryza sativa |
| free fatty acids (FFAs) |
stimulate |
H+ pump activity |
Arabidopsis thaliana |
| HKT, HAK, KAT, CNGC, GLR |
are involved in transporting |
cations |
Oryza sativa |
| HAK high affinity K+ transporters |
are competitively inhibited by |
Na+ |
Oryza sativa |
| K m estimated for Na+ -dependent H+ efflux in potato cell lines |
is of same order of magnitude as |
K m of VvNHX1 transporter and exchangers from Arabidopsis thaliana and Mesembryanthemum crystallinum |
Solanum tuberosum; Vitis vinifera; Arabidopsis thaliana; Mesembryanthemum crystallinum |
| pore domain |
controls |
ion permeation |
|
| variable unitary conductances |
reported for |
Beta vulgaris taproot |
Beta vulgaris |
| plant 14-3-3 proteins |
mediate regulation of |
plasma membrane localized H+-ATPase |
|
| energy required to drive ion transport across plant root membranes |
can comprise |
majority of cellular energy budgets |
|
| (ATCAX3, ATHCX1, CAX1-LIKE, CAX3, AT3G51860) |
is |
tonoplast Ca2+/H+ antiporter |
|
| PutHKT2;1 |
is permeable to |
K+ |
Panicum tenuiflora |
| some HAK transporters |
may also transport |
Na+ |
Hordeum vulgare |
| Na+ -dependent dissipation of pre-established pH gradient |
was used to measure |
Na+ /H+ exchange in tonoplast vesicles |
Solanum tuberosum |
| NPCs |
can be gated for |
other inorganic ions |
|
| intracellular membranes |
contain |
ion channels |
|
| SV channels |
permeate |
magnesium (Mg2+) |
Arabidopsis thaliana; Hordeum vulgare; Beta vulgaris; Plantago maritima; Allium cepa |
| Ca2+ |
blocks |
ion uptake |
|
| Inhibition of Ni2+ tracer uptake by non-radioactive Mg2+ or other cations |
represents |
uptake efficiency of Mg2+ or other cations |
Salmonella |
| shift in E m towards less depolarization or even hyperpolarization |
should decrease |
K + efflux or even induce K + influx |
Arabidopsis thaliana |
| cyclic-nucleotide-gated Ca2+ channel (ATCNGC2, CNGC2, DND1, AT5G15410) |
is known to conduct |
Li+ |
Arabidopsis thaliana |
| P-type ATPases |
release |
protons into apoplast |
Arabidopsis thaliana |
| Cu2+, Mn2+ and Co2+ |
concentrations required for 50% inhibition were in the range of |
100 uM to 10 mM |
Salmonella |
| plant ion channels |
are recorded from |
vacuole |
|
| PM H + -ATPase |
creates |
electrochemical gradient across the PM |
|
| Nepenthes ampullaria at pH 4 |
demonstrates |
H+ uptake in most zones |
Nepenthes ampullaria |
| Ca2+ |
reduces |
bypass flow in rice roots |
Oryza sativa |
| inward currents measured with chloride |
decreased at higher pH but only between pH 4.5 and 5.5 |
external pH |
Xenopus laevis |
| (ATCNGC11, CNGC11, AT2G46440) 12, and /12 |
can function as |
K+ channels |
|
| K+ transporter HvHKT2;1 |
is |
candidate for mediating Ammonium (NH4+) transport |
Hordeum vulgare |
| TEA+ and Gd3+ |
suggest role for |
NSCCs in NH4+ uptake |
|
| continuous uptake of NH4+ into cell versus diffusion of NH3 out of cell |
would result in |
futile cycling of H+ across plasma membrane |
|
| decreased root sodium ion concentrations despite no additional root growth |
may suggest |
direct effects on selective ion transport systems |
Solanum lycopersicum |
| rooted leaves propagated from 140 Ruggeri and K 51-40 grapevines |
were used to compare differences in |
Cl− uptake and transport |
|
| PIPs |
regulate |
ion channel activity |
|
| (ATMGT10, GMN10, MGT10, MRS2-11, AT5G22830) |
shares similar transport properties with |
(ATMGT1, MGT1, MRS2-10, AT1G80900) |
Salmonella |
| alr104 mutant |
shows depolarized plasma membranes during |
combined low-pH/Al treatment |
Arabidopsis thaliana |
| barley potassium (K+) transporter HvHKT2;1 |
transports |
ammonium (NH4+) |
Hordeum vulgare |
| NH4+ transporters of AMT family |
are highly specific for |
NH4+ over K+ |
|
| plasma membrane transporters for active extrusion of NH4+ |
have not been identified in |
plants |
|
| K+ and Na+ |
enter the shoot by distinct mechanisms which are genetically regulated |
distinct mechanisms |
Oryza sativa |
| rice plants |
exhibit enormous individual variability in |
bypass flow and Na+ uptake |
Oryza sativa |
| single-channel fluctuations |
were recorded in |
vacuolar side-in membrane patches |
Arabidopsis thaliana |
| SV channels in guard cells |
differ from |
SV channels in mesophyll cells |
Arabidopsis thaliana |
| blue light pulse |
led to rapid increase in |
K+ in channel activity in wild-type GCPs |
Arabidopsis thaliana |
| polyamines |
are known to trigger |
depolarisation of the plasma membrane |
Pisum sativum; Zea mays; Arabidopsis thaliana |
| similar kinetics of inhibition in MM281 mutant cells and AtMGT5-transformed cells |
rules out |
possible role of (ATMGT5, MGT5, MRS2-6, AT4G28580) in the uptake of Cu2+, Mn2+ and Co2+ under physiological conditions |
Salmonella |
| plant ion channels |
are recorded from |
endoplasmic reticulum (ER) |
|
| K+ deprivation |
significantly favoured |
Rb+ flux in the xylem sap (JRb) |
|
| reversal potentials |
were near zero for |
control oocytes and oocytes expressing HvALMT1 bathed in chloride or MES |
Xenopus laevis |
| TaALMT1 expression in oocytes |
conferred inward and outward currents of similar magnitude to |
HvALMT1-induced currents |
Xenopus laevis; Triticum aestivum |
| pH 4.0 |
causes proton pumping out of lumen side in zones B, D, and E in |
Nepenthes rafflesiana |
Nepenthes rafflesiana |
| genome of Arabidopsis thaliana |
contains |
around 20 genes encoding K+-selective transporters |
Arabidopsis thaliana |
| passive diffusion of ammonia (NH3) out of cytoplasm |
could occur into |
vacuole or across plasma membrane into apoplast |
|
| differing membrane potentials in yeast |
might explain |
different kinetic results |
|
| Aluminum ion (Al 3+) |
inhibits |
(ATMGT10, GMN10, MGT10, MRS2-11, AT5G22830) |
|
| NH4+-grown plants |
have significantly enhanced |
potassium (K+) influx under non-steady-state conditions |
Oryza sativa |
| root-to-shoot K+ translocation |
is mediated at least in part by |
(SKOR, AT3G02850) channel |
Arabidopsis thaliana |
| PutHKT2;1 and OsHKT2;1 |
have |
different ion specificity |
Phragmites tenuiflora; Oryza sativa |
| glutamine at position 270 of TaHKT2;1 |
was previously shown to be responsible for |
low affinity Na+ uptake of the protein |
Triticum aestivum |
| major proportion of Na+ that gets into plant roots |
is likely to be transported through |
non-selective cation channels |
Oryza sativa |
| auto-active Arabidopsis NRG1 oligomer |
forms |
plasma membrane-localized Ca2+-permeable pore or channel |
Arabidopsis thaliana |
| tetraethyl ammonium (TEA+) |
reduces |
ammonium (NH4+) influx |
Hordeum vulgare; Arabidopsis thaliana |
| membrane depolarization |
promotes |
anion efflux |
|
| anionic transport |
plays fundamental roles in |
plant cell biology |
|
| plant ion channels |
are recorded from |
chloroplasts |
|
| K + influx together with E m hyperpolarization in the Al-sensitive mutants |
might have been the result of |
hyperpolarization-activated K + inward-rectifying channels (KIRCs) |
Arabidopsis thaliana |
| magnitude of inward current in oocytes expressing HvALMT1 with malate |
decreased at higher pH |
external pH |
Xenopus laevis |
| malate uptake by oocytes |
was monitored by |
bathing oocytes in [14C]malate and measuring radioactivity |
Xenopus laevis |
| candidate genes for Sr2+ enrichment |
include |
depolarization-activated outward-rectifying K+ channels (KORCs) |
Arabidopsis thaliana |
| entry of Na+ into shoots under these conditions |
is reduced by almost an order of magnitude compared with |
uptake on directly exposing plants to 200 mM NaCl |
Oryza sativa |
| long-distance transport of Na in the plant |
is similar to |
transport of other alkaline ions |
|
| K+ efflux peak value at 100 mM NaCl |
reached |
−600 nmol m−2 s−1 |
|
| malate efflux |
was not significantly different from control oocytes when external pH was |
7.5 rather than 4.5 |
Xenopus laevis |
| K+ stimulation of NH4+ influx in Arabidopsis |
may be related to |
growth conditions |
Arabidopsis thaliana |
| full-length HvHKT2;1 |
best supported growth of |
Δmep1-3 and Δmep1-3 Δtrk1,2 yeast mutants |
Hordeum vulgare |
| ΔN62-hkt2;1 |
complemented better in |
Δtrk1,2 yeast mutant |
Hordeum vulgare |
| stimulation of inward and outward currents upon LiCl addition |
was weaker than |
stimulation by KCl |
Xenopus laevis |
| OsHKT1;1 |
displayed strong inward rectification |
oocytes |
Oryza sativa; Xenopus laevis |
| cell–cell path |
includes |
transport of ions through the plasmodesmata |
Oryza sativa |
| MM281-AtMGT10 cells |
amount of 63Ni2+ did not change significantly after |
efflux assay in the presence of different Mg2+ concentrations |
Salmonella |
| Al tolerance in the alr104 mutant |
correlates with |
higher H+ influx |
Arabidopsis thaliana |
| H+ and K+ net fluxes |
was measured to assess |
Al susceptibility or tolerance of Arabidopsis mutants |
Arabidopsis thaliana |
| proton efflux with K+, NH4+, or combination of both ions |
is measured in |
root cells |
Arabidopsis thaliana |
| increasing shoot levels of bioactive cytokinins |
decreased |
sodium ion concentrations under salinity |
Solanum lycopersicum |
| FFAs |
can modulate |
Plant Inner Membrane Anion Channel (PIMAC) |
|
| MM281 cells |
amount of 63Ni2+ did not change significantly after |
efflux assay in the presence of different Mg2+ concentrations |
Salmonella |
| release of organic anions from the Arabidopsis roots upon Al exposure |
would depolarize |
E m |
Arabidopsis thaliana |
| anion selectivity |
follows the order |
fumarate > malate > citrate > Cl– = MES |
Xenopus laevis |
| epidermal cells beneath lunate cells in Nepenthes rafflesiana |
are candidates for |
active transport of aqueous ions in wax-covered, non-glandular zones |
Nepenthes rafflesiana |
| opposite electrochemical gradients |
require |
different transport mechanisms for uptake and efflux |
|
| 45Ca2+ uptake analysis |
showed |
K927 mutant yeast with (ATCNGC11, CNGC11, AT2G46440) /12 or /12:R557C had higher uptake rate of 45Ca2+ than K927 carrying empty vector |
|
| TEA+ inhibition of NH4+-induced H+ efflux |
supports interpretation that |
activity of plasma membrane H+-ATPase represents energy-consuming step |
Arabidopsis thaliana |
| electrical potentials at membrane surfaces (ψ 0) |
influences |
driving force for ion fluxes across membranes |
|
| transporters and channels |
might be involved in |
Cs+ and Sr2+ accumulation processes |
|
| two parallel pathways for Ca2+/Sr2+ movement to the xylem |
could imply |
functional separation of symplastic and apoplastic Ca2+/Sr2+ flux within the root |
Arabidopsis thaliana |
| ABA inhibition of guard cell H+ ATPase activity |
contributes to |
membrane depolarization |
|
| FFA ionophoric activity |
was shown to be negligible in |
K+ permeability enhancement |
|
| Aliquots of cells with equal amount of radioactivity |
were transferred in |
efflux assay buffer containing different Mg2+ concentrations |
Salmonella |
| plant ion channels |
are recorded from |
plasma membrane |
|
| prolonged K+ deprivation (31 h) |
caused marked increase in |
K+ flux in the xylem sap (JK) |
|
| als5 mutant |
shows initial depolarization followed by hyperpolarization during |
combined low-pH/Al treatment |
Arabidopsis thaliana |
| change in E m |
would theoretically affect |
K + flux |
|
| MZ |
has |
larger number of K + transport systems than the root apex |
Arabidopsis thaliana |
| anion and cation channels in stelar cells |
have been detected and |
characterized |
|
| (ATCNGC11, CNGC11, AT2G46440) /12 channel activity |
is attributable for |
cpr22 phenotype |
Arabidopsis thaliana |
| futile cycling of H+ across plasma membrane |
would be catalysed by |
ATP-fuelled plasma membrane H+-ATPase |
|
| BOA |
inhibits |
plasma membrane bound H+ ATPases |
Avena fatua |
| (SKOR, AT3G02850) |
is |
selective K+ outward-rectifying channel |
|
| latent Mg2+-sensitive ion-conducting pathways |
include |
K+ transporter |
|
| study of durum wheat (ATHKT1, HKT1, HKT1;1, AT4G10310) ;4 transporters |
shows that important functional variability exists among |
durum wheat (ATHKT1, HKT1, HKT1;1, AT4G10310) ;4 genes |
Triticum turgidum subsp. durum |
| K+ leakage mediated by K+-permeable channels |
is |
focus of this article |
|
| root proton extrusion in OES/WTR |
shows no significant difference with |
root proton extrusion in MNS/WTR |
Arabidopsis thaliana |
| Al-induced E m depolarization in the Al-tolerant genotypes |
could be a result of |
currents caused by the H + flux across the plasma membrane |
Arabidopsis thaliana |
| currents |
were measured at |
pH 4.5, 5.5, and 7.5 |
Xenopus laevis |
| K+ transporter (ATKCO1, ATTPK1, KCO1, TPK1, AT5G55630) |
is |
candidate for mediating Ammonium (NH4+) transport |
Arabidopsis thaliana |
| increased channel or transporter activity for K+ uptake |
may result in |
increased NH4+ influx |
Arabidopsis thaliana |
| membrane depolarization |
suppresses |
inward K+ channel opening |
|
| contact of phenolic acids with root cell membrane |
leads to |
efflux of ions |
|
| (ATMGT10, GMN10, MGT10, MRS2-11, AT5G22830) uptake pattern |
is in sharp contrast with |
(ATMGT5, MGT5, MRS2-6, AT4G28580) inhibition of tracer uptake pattern |
Salmonella |
| Cu2+, Mn2+ and Co2+ |
significantly inhibited |
tracer uptake in AtMGT5-transformed cells |
Salmonella |
| shift in K + flux towards influx |
might be due to |
direct or indirect effect of (ALS3, AT2G37330) and (AHAS, ALS, CSR1, IMR1, TZP5, AT3G48560) mutations |
Arabidopsis thaliana |
| immediate difference between the wild type and (ALS3, AT2G37330) mutants in ion fluxes and E m after exposure to Al |
indicates that |
(ALS3, AT2G37330) functioning may be linked to maintenance of E m depolarization, K + efflux, H + influx, and, in the longer term, to K + homeostasis |
Arabidopsis thaliana |
| magnitudes of inward and outward currents |
were between 2- and 5-fold greater with |
malate, fumarate, or citrate in the bathing solution |
Xenopus laevis |
| Arabidopsis thaliana (AKT1, ATAKT1, KT1, AT2G26650) |
transports |
ammonium (NH4+) |
Arabidopsis thaliana |
| NH4+ uptake |
is reduced by |
K+ |
Arabidopsis thaliana |
| HKT isoforms in various species |
have been shown to function as |
K+/Na+ co-transporters |
|
| E. coli AmtB |
binds NH4+ with high affinity |
NH4+ |
Escherichia coli |
| NH3 permeable tonoplast intrinsic proteins (TIPs) |
are present in |
vacuole |
|
| candidate genes for Sr2+ enrichment |
include |
ionotropic glutamate receptors (GLRs) |
Arabidopsis thaliana |
| AtMGT5-mediated influx |
is specifically inhibited by |
high concentration of magnesium |
Salmonella |
| higher H+ uptake under low-pH stress in als5 mutant |
is abolished by |
Al exposure |
Arabidopsis thaliana |
| net NH4+ and K+ fluxes into roots |
show almost linear inverse correlation |
NH4+ and K+ concentrations |
Arabidopsis thaliana |
| short term K+ deprivation (7 h) |
occurs independently of its effect on |
K+ flux in the xylem sap (JK) |
|
| K + influx or decreased K + efflux |
was observed in |
DEZ of all the genotypes tested |
Arabidopsis thaliana |
| TEA+ |
strongly reduces |
NH4+ influx |
Arabidopsis thaliana |
| small changes in the HKT protein structure |
could be expected to alter |
ion selectivity or affinity of the protein |
|
| mutation of the glutamine residue to leucine |
resulted in reduced |
low affinity Na+ uptake of TaHKT2;1 |
Triticum aestivum |
| transporters |
mediate |
Mg 2+ uptake |
|
| CorA-like proteins |
have been identified from |
animals and human |
animals; human |
| plasma membrane surface potential differences between Arabidopsis genotypes exposed to low-pH/Al treatment |
could have been linked to |
greater H + influx inhibition and enhanced H + efflux in the Al-sensitive mutants ( (ALS3, AT2G37330) and als5) compared with the wild type and the Al-tolerant alr104 mutant |
Arabidopsis thaliana |
| Al inhibition of Arabidopsis protein kinase (CIPK11, PKS5, SIP4, SNRK3.22, AT2G30360) |
inducing |
acidification of the external medium |
Arabidopsis thaliana |
| active transport of NH4+ across wax-covered zones D and E |
was found to occur in |
Nepenthes fusca pitchers |
Nepenthes fusca |
| Arabidopsis thaliana (AKT1, ATAKT1, KT1, AT2G26650) |
uptake of potassium (K+) is inhibited by increasing concentrations of |
ammonium (NH4+) |
Arabidopsis thaliana |
| CYCLIC-NUCLEOTIDE-GATED CHANNEL 1 (ATCNGC1, CNGC1, AT5G53130) |
contains polymorphism affecting amino acids close to |
K+-pore of CYCLIC-NUCLEOTIDE-GATED CHANNEL 1 (ATCNGC1, CNGC1, AT5G53130) |
Arabidopsis thaliana |
| (ATKT1, ATKT1P, ATKUP1, KT1, KUP1, AT2G30070) |
heterologous expression in yeast yielded different kinetic results compared with |
in planta studies |
Arabidopsis thaliana |
| apoplastic transpirational bypass flow of water and solutes |
plays a significant role in |
Na+ uptake in rice |
Oryza sativa |
| response to salt |
occurred before |
Na+ or Cl- entry into shoot |
Triticum turgidum |
| K+/H+ exchanger |
is very active in |
plant mitochondria |
|
| tip-focused plasma membrane H+ adenosine triphosphatase (PM H+-ATPase) |
might lead to |
extrusion of H+ from the tube tip |
Torenia fournieri |
| Nax1 (TmHKT1;4-A2) |
could unload Na+ from |
xylem of the root and leaf sheath |
Triticum turgidum |
| TmHKT1;4-A2 expression pattern in roots and leaf sheath |
was consistent with |
its proposed physiological role in removing Na+ from the xylem of the roots and leaf sheaths |
Triticum turgidum |
| Nax2 and Kna1 phenotype |
includes |
enhanced discrimination of K+ over Na+ in transport from roots to shoots |
Triticum turgidum; Triticum aestivum |
| high-affinity potassium (K+) transport mechanism in rice |
may have greater binding affinity for |
potassium (K+) |
Oryza sativa |
| ammonium (NH4+)-resistant potassium (K+) transport via channels |
may occur at lower external concentration in |
rice |
Oryza sativa |
| high external Na+ |
favours |
Na+ entry down the electrochemical gradient across the plasma membrane |
|
| PutHKT2;1 |
shows K+-Na+ transport characteristics similar to |
PhaHKT2;1 |
Panicum tenuiflora; Phragmites australis |
| OsCHX11 |
has closest homologue in Arabidopsis as |
(ATCHX17, CHX17, AT4G23700) |
Oryza sativa; Arabidopsis thaliana |
| ozone |
can directly affect guard cells by inhibiting |
inward K+ channels |
Trifolium subterraneum |
| Magnesium/proton exchanger (FG498083) |
exchanges protons with |
Mg2+ and Zn2+ ions |
|
| no specific pattern of K + flux changes |
was observed in the MZ under |
low-pH or combined low-pH/Al stress |
Arabidopsis thaliana |
| rate of malate uptake |
was significantly greater in |
oocytes expressing HvALMT1 than in control oocytes at pH 4.5 |
Xenopus laevis |
| (ATCNGC1, CNGC1, AT5G53130) |
is known to be involved in |
metal uptake |
Arabidopsis thaliana |
| Nrat1 (Nramp aluminium transporter 1) |
is |
putative plasma membrane-localized Al transporter |
Oryza sativa |
| long- and short-term effects of salt stress on K+ fluxes from quinoa roots |
should be addressed in future experiments by comparing |
temporal dynamics of K+ efflux |
|
| FFAs |
can modulate |
mammalian mitochondrial K+ channel |
|
| plant mitochondrial ATP-sensitive potassium channel (PmitoK ATP) activation under stress conditions |
may collapse |
electrical membrane potential (ΔΨ) |
|
| genes encoding Na+ efflux proteins |
have been detected mainly in |
Arabidopsis |
Arabidopsis thaliana |
| decreased root sodium ion concentrations despite no additional root growth |
may suggest |
indirect effects via membrane potential changes |
Solanum lycopersicum |
| OsHKT1;1 |
mediated |
low-affinity Na+ uptake in a transformed yeast system |
Oryza sativa |
| Na+ transport function of PutHKT2;1 |
is partially dependent on |
[Ca Ext+] |
Panicum tenuiflora |
| K m of Na+ -dependent H+ efflux in Mesembryanthemum crystallinum |
is |
44–51 mM Na+ |
Mesembryanthemum crystallinum |
| plant ion channels |
are recorded from |
mitochondria |
|
| Al-induced decrease in H+ influx at the distal elongation zone (DEZ) |
is higher in |
(ALS3, AT2G37330) and als5 mutants |
Arabidopsis thaliana |
| K+ efflux at the distal elongation zone (DEZ) |
corresponds to |
changes in plasma membrane potential (Em) |
Arabidopsis thaliana |
| simultaneous action of HATS and LATS |
produces |
apparently (curvi-) linear relationships due to external concentration |
|
| CNBD mutant S73 (E519K) |
affects |
ion channel function |
|
| AmtB structure |
suggests |
discrimination of K+ against NH4+ in AMT homologues is due to de-protonation process |
|
| melon rootstock |
has nearly no Na exclusion |
sodium (Na) ion |
|
| H+-ATPase activation |
prevents |
further K+ leak from cytosol |
|
| SKC1 (OsHKT1;5) |
could unload Na+ from |
xylem of the root |
Oryza sativa |
| ammonium (NH4+) nutrition at low K+ concentrations |
reduces |
potassium (K+) influx |
Oryza sativa |
| more recent work |
suggests that |
K+ channels may mediate Na+ uptake in halophytes |
|
| tonoplast proton pumps |
generate |
proton-motive force |
|
| primary H+ pumps |
require co-action of |
other ion transporters |
|
| weak shifts of zero-current potential upon KCl addition |
confirmed that |
K+ transport through the two TdHKT1;4 transporters was very limited |
Xenopus laevis |
| slow-type (S-type) anion channels |
are present in |
Vicia faba guard cells |
Vicia faba |
| ES4 |
leads to opening of |
maize potassium channel KZM1 |
Zea mays |
| magnitudes of outward currents in oocytes bathed in malate, chloride, or MES |
were less dependent on |
external pH |
Xenopus laevis |
| electrical gradients |
have opposite effects on |
cations and anions |
|
| symplastic pathway |
is more prominent at |
low [Ca2+] medium |
Arabidopsis thaliana |
| organic anions malate and citrate |
participate in regulation of |
uptake of other ions |
|
| transporters |
mediate |
Mg 2+ efflux |
|
| efflux assay |
tested |
whether (ATMGT5, MGT5, MRS2-6, AT4G28580) mediates Mg2+ efflux under high Mg2+ conditions |
Salmonella |
| H + -ATPase |
decreased activity of |
H + influx under low-pH conditions |
Arabidopsis thaliana |
| positively charged plasma membrane surface |
would impede |
uptake of cations, including H + ions |
Arabidopsis thaliana |
| release of smaller amounts of citrate from Al-sensitive genotypes |
would diminish |
E m depolarization |
Arabidopsis thaliana |
| Al3+ treatment |
was absent from |
cells treated with La3+ |
Xenopus laevis |
| magnitudes of outward currents |
were dependent on |
anion in the bathing solution |
Xenopus laevis |
| (ATCNGC11, CNGC11, AT2G46440) /12:R557C |
is functional as |
Ca2+ ion channel |
|
| co-supplementing plants with K+ |
increases |
NH4+ influx |
Arabidopsis thaliana |
| growth depression of barley plants at high NH4+ |
was suggested to be due to |
futile cycling of NH4+ |
Hordeum vulgare |
| phenylvalerate |
stimulates |
depolarization induced by low KCl addition |
|
| glucose application |
had no effect on |
membrane potential of the plasma membrane of giant cells |
Impatiens balsamina |
| depolarized membranes |
inhibit |
secondary energy-dependent ion transport |
Triticum aestivum |
| vanadate |
is known blocker of |
plasma membrane H+-ATPase pump |
|
| barley seedlings at 0.1 mM [K+]ext with NH4+ |
show suppressed |
potassium (K+) influx |
Hordeum vulgare |
| ammonium (NH4+) |
stimulates |
low-affinity potassium (K+) influx |
Oryza sativa |
| barley seedlings under low K+ and high N conditions |
show |
ammonium (NH4+) suppression of shoot K+ transport by 90% |
Hordeum vulgare |
| Na+ transport function of OsHKT2;1 |
is independent of |
[Ca Ext+] |
Oryza sativa |
| mode of action of sorgoleone |
is suggested to involve |
inhibition of root H+-ATPase activity |
|
| malate efflux from oocytes |
was monitored after |
oocytes were injected with [14C]malate |
Xenopus laevis |
| highest H+ efflux |
is measured in presence of |
both NH4+ and K+ |
Arabidopsis thaliana |
| H+ efflux |
is reduced upon co-presence of |
TEA+ and Gd3+ |
Arabidopsis thaliana |
| excess NH4+ continuously provided from external medium |
could diffuse in form of |
uncharged NH3 along concentration gradient |
|
| ion transport across PMs |
often correlates poorly with |
ion activity in bulk-phase medium |
|
| stem exudate Na concentration in plants with melon rootstocks |
is similar to |
Na concentration in irrigation water |
Cucumis melo L.; Cucurbita maxima Duchesne; Cucurbita moschata Duchesne |
| Na+ |
was |
the most permeant cation |
Xenopus laevis |
| quinine sensitivity of TdHKT1;4-2 |
encourages |
further examination of quinine sensitivity within the HKT family |
|
| membrane-bound transporter protein |
facilitates |
NH4+ ion movement from cell to cell |
Oryza sativa |
| K+ channels |
are inhibited by |
human β-defensin 2 |
|
| (ACD6, DEG16, AT4G14400) ion channel activity |
can be further enhanced by |
MHA1L |
Xenopus laevis; Homo sapiens |
| mechanosensitive (MS) ion channels |
mediate |
ion flux across the membrane |
|
| residual negative charges in cell walls |
may lead to |
accumulation of some ions |
Triticum aestivum |
| absence of alkalization |
is observed in |
Arabidopsis thaliana cells in previous studies |
Arabidopsis thaliana |
| decrease of plasma membrane H+-ATPase activity |
could be responsible for |
alkalization of the external medium |
Arabidopsis thaliana |
| IAA (indole acetic acid) |
is known to stimulate |
H+-ATPase activity |
|
| addition of 10 μM IAA in the presence of 10 μM TXT |
did not counteract |
alkalization |
Arabidopsis thaliana |
| K+ influx enhancement by NH4+ at 0.1 mM [K+]ext |
is only temporary when |
seedling transfer from 0.1 mM to higher [K+]ext |
Oryza sativa |
| transport properties of an HKT protein |
may not be solely determined by |
single residues |
|
| TaHKT2;1 (TaHKT1) |
mediates |
Na+ influx |
Triticum aestivum |
| OsHKT1;5 (SKC1) |
functions as |
Na+-selective transporter |
Oryza sativa |
| rapid depletion of mitochondrial Mg2+ |
activates |
latent Mg2+-sensitive ion-conducting pathways |
|
| La3+ pretreatment |
avoids |
TXT-induced plasma membrane depolarization |
Arabidopsis thaliana |
| OsHKT2;1 |
functions as |
Na+ selective uniporter |
Oryza sativa |
| sorgoleone |
can inhibit |
root H+-ATPase activity |
|
| tonoplast Na+/H+ antiporters |
are similar to |
Na+/H+ exchanger |
|
| long-chain free fatty acids (FFAs) |
activate |
latent Mg2+-sensitive ion-conducting pathways |
|
| direct Mg2+ withdrawal from protein-binding sites |
activates |
latent Mg2+-sensitive ion-conducting pathways |
|
| changes in the electrical driving force for ion transport across the PM |
influenced |
relative root elongation rate (RRER) |
Vigna unguiculata L. Walp. |
| plasma membrane H+ adenosine triphosphatase (PM H+-ATPase) moving with cytoplasmic streaming back from the tip |
would trigger |
excretion of H+ into the cell wall |
|
| K+ utilizing induced NH4+ transporter |
accounts for |
increased potassium (K+) flux under K+ LATS conditions |
Oryza sativa |
| root cytosolic K+ concentration |
shows high correlation with |
shoot potassium (K+) content |
Oryza sativa |
| OsHKT2;1 |
mediates |
Na+-uptake into K+-starved roots |
Oryza sativa |