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gene silencing

19129 relationships annotated with this phrase. Showing first 500 of 19129.
Source entity Relationship Target entity Species
gene silencing of tale genes was equally potent for all the tale genes of Xpm Xanthomonas phaseoli
heterochromatin represses gene expression
tobacco rattle virus (TRV) VIGS used to knock down ISP-F and (AtCLA1, CLA, CLA1, DEF, DXPS2, DXS, DXS1, AT4G15560) genes in the MEP pathway Arabidopsis thaliana
virus-induced gene silencing (VIGS) used to silence SlMAP3K18, SlMAP2K2, and SlMAP2K4 genes Solanum lycopersicum
(MIR399, MIR399F, AT2G34208) * duplex size did not affect silencing efficiency of (MIR399, MIR399F, AT2G34208) Arabidopsis thaliana
DNA methylation in fungus is associated with silencing of gene expression and transposon
callose deposition in i-cals3m rootstock inhibited (ATUBC24, PHO2, UBC24, AT2G33770) suppression Arabidopsis thaliana
VIGS signal is confined to silenced fruits Solanum lycopersicum
PSY3 construct cross-silenced weakly PSY2 and (PSY1, AT5G58650) genes Solanum lycopersicum
(CCD4, NCED4, AT4G19170) gene expression was down-regulated using RNA interference Solanum tuberosum
DNA methylation influences inhibition of aberrant transcription
HIGS-RiMsn2-RNAi roots have significantly reduced transcription of RiMsn2 Medicago truncatula; Rhizophagus irregularis
short tandem target mimic (STTM) technology is employed to investigate functions of small RNAs
RNAi application never resulted in complete inhibition of (AAD6, FTM1, HUP7, SAD6, AT1G43800) expression Arabidopsis thaliana
triple mutants were constructed by down-regulating expression of the third gene family member in an inducible manner Arabidopsis thaliana
(MIR319, MIR319B, AT5G41663) targets OsGAMYB Oryza sativa
transgenic Nicotiana benthamiana lines with reduced expression of individual (CCT, CRP, MED12, AT4G00450) subunits are generated with 35S-driven and (CCT, CRP, MED12, AT4G00450) subunit-specific hairpin constructs Nicotiana benthamiana
pTRV-SlBBX17 plants show reduced transcript levels of Transcription factor B-box 17 (SlBBX17) Solanum lycopersicum
Argonaute (AGO) facilitates translational repression translation
GhPMEI3 transcript levels are considerably higher in completely and partially silenced cotton plants compared to control plants Gossypium hirsutum
VEF domain is found in chromatin proteins required for gene silencing
NlVg gene is silenced by RNAi Nilaparvata lugens
RNA interference (RNAi) represses target gene expression
NbAPX3-1 was silenced in Nicotiana benthamiana Nicotiana benthamiana
virus-induced gene silencing (VIGS) strategy can be used to silence candidate genes
geraniol synthase gene was silenced in planta using virus-induced gene silencing Solanum melongena
relative expression level of MpFPPS1 significantly reduced by c. 20% after feeding transgenic plants for 5 d MpFPPS1 expression Myzus persicae; Nicotiana tabacum
PSY2 and PSY3 were silenced less efficiently 70%–75% Solanum lycopersicum
transgenic YFP-HSC70.1 and YFP-HSC70.1M hsc70.1 hsp70.4 double mutants showed high degree of patchy YFP fluorescence Arabidopsis thaliana
Sm GS-silenced RL22 plants show >20-fold lower Sm GS transcript abundance Solanum melongena
(MIR399, MIR399F, AT2G34208) expressed in dcl2-1/4-2 mutant scion showed suppression of rootstock (ATUBC24, PHO2, UBC24, AT2G33770) Arabidopsis thaliana
silencing KMT1 might be less pronounced than complete inactivation of KMT1 Leptosphaeria maculans
BSMV:TaRCA significantly reduces expression of TaRCA Triticum aestivum
single guide RNAs (sgRNAs) designed against Xanthomonas phaseoli pv manihotis TALE conserved gene sequences efficiently silenced expression of transcription activator-like effector (TALE) Xanthomonas phaseoli pv manihotis
single sgRNA targets highly conserved sequence in promoters or 5′-UTR regions Xanthomonas
abrogation of histone acetylation mark ensures no leaking gene expression occurs
(MIR166, MIR166G, AT5G63715) ectopic expression leads to silencing of (MIR166, MIR166G, AT5G63715) target genes Oryza sativa
longer maintenance of inserts is correlated with greater target gene silencing
pEGAD MYB5:GFP–cHDG2 transgene causes co-suppressed hdg2-like mutant papillae phenotype Arabidopsis thaliana
RNA polymerase V ( (POL, AT2G46920) V) may play role in gene silencing via one or more pathways distinct from 24-nt siRNA-directed pathway Arabidopsis thaliana
loci with considerable or complete sequence identity can acquire different combinations of epi-regulators for gene silencing Arabidopsis thaliana
barley stripe mosaic virus hordeivirus (BSMV) was adapted for virus-induced gene silencing to study gene function in monocots
PcG-mediated silencing requires additional mechanisms Arabidopsis thaliana
other genes in the study were silenced with efficiency of 85%–100% Solanum lycopersicum
Os04g48410 exhibits negative correlation to miR398 abundance Oryza sativa
transgenic lines with high (FOC, MIR160, MIR160A, AT2G39175) expression show reduced expression of target genes Os02g41800 and Os04g43910 Oryza sativa
qRT-PCR analysis of tissues infected with BMVCP5:HSP70-1 using primers for (AtHsp70-2, Hsp70-2, AT5G02490) indicated (AtHsp70-2, Hsp70-2, AT5G02490) was silenced Zea mays
RNAi construct targeting (CLF, ICU1, SDG1, SET1, AT2G23380) has clone ID CD3-538
forward genetic screen identified METHIONINE ADENOSYLTRANSFERASE 4 (AtSAMS3, MAT4, MTO3, SAMS3, AT3G17390) Arabidopsis thaliana
myosin silencing occurs in plants inoculated with VIGS vector Nicotiana tabacum
transgenic lines with high (MIR398B, AT5G14545) expression show greatly suppressed target genes Os03g22810, Os04g48410, Os07g46990, and Os11g09780 Oryza sativa
(AtSAMS3, MAT4, MTO3, SAMS3, AT3G17390) / (Met overaccumulation) mutant releases silencing of both genes Arabidopsis thaliana
antisense and RNAi-mediated silencing of (AtMYB62, BW62B, BW62C, MYB62, AT1G68320) did not suppress (AtMYB62, BW62B, BW62C, MYB62, AT1G68320) expression
TRV:HIPP26 showed expression of targeted (ATFP6, AtHMP40, FP6, HIPP26, AT4G38580) gene was reduced Nicotiana benthamiana
OsGAMYB was obviously suppressed by coexpression with (MIR319, MIR319B, AT5G41663) Nicotiana benthamiana
LUC gene expression in line CCT383 is highly sensitive to suppression of (MOM, MOM1, AT1G08060) expression Arabidopsis thaliana
HLP mRNA accumulation is down-regulated to levels below detection limit in HLP–RNAi-43 strain Chlamydomonas reinhardtii
BSMVγ–TaPDS construct substituted for BSMVγ–ZoPDS Zingiber officinale; Triticum aestivum
ihpRNA–LeHT3 single copy lines knocked down LeHT1 expression Solanum lycopersicum
PAR-RNAi transgenic plants effectively suppresses transcript abundance of (HLH1, PAR1, AT2G42870) Arabidopsis thaliana
PAR-RNAi transgenic plants effectively suppresses transcript abundance of (ADH2, ATGSNOR1, GSNOR, HOT5, PAR2, AT5G43940) Arabidopsis thaliana
BMVCP5 vector better maintained heat shock protein70-1 (AT-HSC70-1, AtHsp70-1, HSC70, HSC70-1, HSP70-1, AT5G02500) insert Nicotiana benthamiana; Zea mays
knockdown of (PDE226, PDS, PDS3, AT4G14210) expression in second and third systemically infected leaves at 14 dpi with BMVCP5 is approximately twice relative levels observed with BMVF13m:PDS Zea mays
repressive chromatin environment at (AGL25, FLC, FLF, RSB6, AT5G10140) locus represses (AGL25, FLC, FLF, RSB6, AT5G10140) expression Arabidopsis thaliana
leaf disc screening identified NAD-ME RNAi lines Kalanchoe fedtschenkoi
TRV-based VbMS by overexpressing miRNA target mimics was efficient to silence plant endogenous miRNAs
Brome mosaic virus (BMV)-based vector better maintains foreign gene inserts
(MIR319, MIR319B, AT5G41663) suppresses OsTCP21 Oryza sativa
eight epi-regulators ( (AtLHP1, LHP1, TFL2, AT5G17690) (MOM, MOM1, AT1G08060) (CMT3, AT1G69770) DEFECTIVE IN RNA-DIRECTED DNA METHYLATION 1 (CHR35, DMS1, DRD1, AT2G16390) (DMT7, DRM2, AT5G14620) Su(VAR)3-9 HOMOLOG 2 (ATSUVH2, SDG3, SUVH2, AT2G33290) CURLY LEAF (CLF, ICU1, SDG1, SET1, AT2G23380) and HISTONE DEACETYLASE 1 (ATHD1, ATHDA19, HD1, HDA1, HDA19, HDAC19, RPD3A, AT4G38130) ) are compared by RNAi-mediated silencing Arabidopsis thaliana
myosin XI VIGS vector reduces abundance of myosin XI transcripts Nicotiana tabacum
transformed algal clones screened for down-regulated HLP mRNA accumulation Chlamydomonas
DNA methylation coregulates heterochromatin formation
Arabidopsis (AtMORC1, CRT1, MORC1, AT4G36290) and (AtMORC6, DMS11, MORC6, AT1G19100) implicate in gene silencing Arabidopsis thaliana
expression level of the hairpin RNAi transgene in line rNAD-ME2 may have been sufficient to drive degradation of all β-NAD-ME transcripts Kalanchoë fedtschenkoi
120-bp fragment of NbHIPP26 was cloned into TRV in antisense orientation Nicotiana benthamiana
qRT-PCR analysis of tissues infected with BMVCP5: (ATHSP70, HSC70-4, HSP70, HSP70-4, AT3G12580) -1 using primers for showed similar result (ATHSP70, HSC70-4, HSP70, HSP70-4, AT3G12580) was silenced Zea mays
OsTCP21 was obviously suppressed by coexpression with (MIR319, MIR319B, AT5G41663) Nicotiana benthamiana
H3K27me3 confers (AGL38, PHE2, AT1G65300) repression Arabidopsis thaliana
modified BSMVγ RNA containing partial sequence of Z. officinale (PDE226, PDS, PDS3, AT4G14210) gene effects down-regulation of endogenous (PDE226, PDS, PDS3, AT4G14210) Zingiber officinale
respective target gene after 7 d of induction was strongly repressed in young, expanding leaves and mature leaves Nicotiana tabacum
RNA silencing (RNAi) can be achieved through stable transformation or transient expression of silence-inducing fragments with virus vectors
tobacco rattle virus (TRV)-based system was used to knock down (ATFP6, AtHMP40, FP6, HIPP26, AT4G38580) expression in N. benthamiana plants Nicotiana benthamiana
screening of transformed algal clones identified clones displaying intermediate levels of down-regulation Chlamydomonas
virus-induced gene silencing (VIGS) is relatively new approach to down-regulate gene expression in plants
virus-induced gene silencing (VIGS) does not result in 100% uniform silencing
SlNAP2 knockdown line KD-L2 exhibits target gene specificity with RNAi Solanum lycopersicum
phloem contains small interfering RNAs
distinct sets of epi-regulators are recruited to four tagged locations in chromosome 2 Arabidopsis thaliana
LUC gene cassette in CC lines CCP4.211 and CCT383 is silenced in shoots and roots Arabidopsis thaliana
CC lines CCT396 and CCT431 exhibit root-specific silencing of LUC expression Arabidopsis thaliana
RNAi construct targeting (ATSUVH2, SDG3, SUVH2, AT2G33290) has clone ID CD3-539
HLP mRNA accumulation is down-regulated to levels below detection limit in HLP–RNAi-39 strain Chlamydomonas reinhardtii
ANP3-specific artificial microRNA (amiRNA) expressed under the control of β-estradiol-inducible promoter Arabidopsis thaliana
small RNAs (sRNAs) consists of small interfering RNA (siRNA) and microRNA (miRNA)
myosin XI-silencing sequences are likely to affect tobacco myosin XIs Nicotiana tabacum
H3K27 trimethylation (H3K27me3) silences or represses target gene expression
CC lines CCP4.211, CCT383, CCT396, and CCT431 contain LUC gene cassette Arabidopsis thaliana
eight epi-regulators are involved in LUC gene silencing Arabidopsis thaliana
Tip5 mediates establishment of rDNA silencing Homo sapiens
4158-bp putative (ABCG11, AtABCG11, ATWBC11, COF1, DSO, WBC11, AT1G17840) gene promoter region is the primary source of co-suppression effect Arabidopsis thaliana
selective knockdown of LeHT3 expression was not achieved ihpRNA–LeHT3 construct Solanum lycopersicum
NbIRX9 transcript level is reduced in VIGS-NbIRX9-infected plants Nicotiana benthamiana
MYC3–RNAi transgenic lines show slight reduction in (MYC3, AT5G46760) Arabidopsis thaliana
RNAi construct covered 211 nucleotides spanning positions 145 to 355 of the coding region Nicotiana tabacum
small RNAs guide gene silencing by regulating chromatin methyl modification
RNAi construct targeting (DMT7, DRM2, AT5G14620) has clone ID CD3-557
stele-expressed P19 antagonizes systemic silencing of (MIR399, MIR399F, AT2G34208) Arabidopsis thaliana
5′-UTR of (ABCG11, AtABCG11, ATWBC11, COF1, DSO, WBC11, AT1G17840) is not responsible for co-suppression of (ABCG11, AtABCG11, ATWBC11, COF1, DSO, WBC11, AT1G17840) Arabidopsis thaliana
recently duplicated Arabidopsis FBX gene members are inactive due to epigenetic suppression Arabidopsis thaliana
Os04g59430 was significantly suppressed in (FOC, MIR160, MIR160A, AT2G39175) overexpression transgenic lines Oryza sativa
three independent rNAD-ME lines showed large reductions in the steady-state transcript level of the targeted β-NAD-ME gene (KfNAD_ME1_b1) Kalanchoë fedtschenkoi
plant viral vectors have been widely used for gene silencing
ethylene response factor 1 (AtERF#092, ERF1, ERF1B, AT3G23240) transgene is often silenced Arabidopsis thaliana
Os04g48390 exhibits negative correlation to miR827 abundance Oryza sativa
Os05g03040 exhibits negative correlation to (EAT, MIR172, MIR172B, AT5G04275) abundance Oryza sativa
ZmSHR1 gene was used to avoid potential silencing of OsSHR2 Oryza sativa ssp japonica
(AtSAMS3, MAT4, MTO3, SAMS3, AT3G17390) missense mutation can release silencing of Pro35S::NPTII Arabidopsis thaliana
repressive histone modifications result in suppression of (AGL25, FLC, FLF, RSB6, AT5G10140) expression Arabidopsis thaliana
PRC1-like complex participates in transcription suppression Arabidopsis thaliana
P. coerulescens antisense PTrx gene directed to the cytosol effectively down-regulates endogenous wheat Trx h9 Triticum aestivum
BSMVγ–ZoPDS plasmid is similar to BSMVγ–TaPDS Zingiber officinale; Triticum aestivum
p (ABCG11, AtABCG11, ATWBC11, COF1, DSO, WBC11, AT1G17840) upstream region is sufficient for generating co-suppression of (ABCG11, AtABCG11, ATWBC11, COF1, DSO, WBC11, AT1G17840) Arabidopsis thaliana
LeHTi–036 line showed no significant effect on LeHT expression levels Solanum lycopersicum
gene silencing mediated by small RNAs (sRNAs) plays a central role in regulation of gene expression in plants
pBI–Fraa1ei-infiltrated fruits strongly reduced to less than 40% of the levels Fra a transcript levels
NbCCR transcript level is reduced in VIGS-NbCCR-infected plants Nicotiana benthamiana
siRNAs mediate transcriptional gene silencing (TGS)
spray-induced gene silencing (SIGS) is crop protection method
ethyl methanesulfonate (EMS) mutagenesis screen is used to investigate factors involved in the susceptibility of transgenes to PTGS
introns in a terminator-dependent fashion can induce siRNA production from transgenes
BSMVγ–TaPDS construct evaluated for effectiveness in VIGS in Zingiber officinale Zingiber officinale; Triticum aestivum
transient RNAi-mediated silencing of the Fra a genes reduces levels of Fra a mRNAs Fragaria x ananassa
transgenes have high susceptibility to silencing
transgene designed to mimic an endogene and containing both the 5′ and 3′ regulatory regions plus introns of the tobacco RuBisCO was shown to be less prone to PTGS and TGS
amiRNA designed to target stem-loop sequence of miRNA precursor transcript silences expression of individually targeted family member Arabidopsis thaliana
VIGS treatment significantly reduced CK2B1 expression in pTY-CK2B1 lines Brassica juncea
Gh_A10G2048 transcription in VIGS plants was significantly suppressed to 24% of control plants expression level Gossypium hirsutum
mitochondrial NAD-dependent isocitrate dehydrogenase (Sl (IDH-I, IDH1, AT4G35260) ) is expressed in antisense orientation in transgenic tomato plants Solanum lycopersicum
NbCOBRA transcript level is reduced in VIGS-NbCOBRA-infected plants Nicotiana benthamiana
RNAi-mediated silencing of (ATPRX Q, PRXQ, AT3G26060) in mutant unable to further reduce 16:1t levels Arabidopsis thaliana
small RNAs (sRNAs) and ARGONAUTE (AGO) proteins form RNA-induced silencing complex (RISC)
303-bp PCR fragment from ACL2 gene inserted into p1301UbiNos vector forms antisense ACL2 vector
partial silencing of (ATPRX Q, PRXQ, AT3G26060) in wild-type background results in mild reduction in 16:1t levels Arabidopsis thaliana
species-specific terminator incompatibility highlights importance of choosing an appropriate 3′ regulatory element for transgene expression
visual reporter (PDE226, PDS, PDS3, AT4G14210) tracks VIGS progress
(POL, AT2G46920) IV-dependent 21/22-nt siRNAs are involved in post-transcriptional gene silencing (PTGS) Arabidopsis thaliana
ihpRNA approach targeting LeHT genes caused substantial reduction in expression levels of all three LeHT genes Solanum lycopersicum
small interfering RNAs (siRNAs) is main sRNA class in plants
silencing constructs were delivered first and effector was delivered later following an interval of 1–2 days Nicotiana benthamiana
heterochromatin is correlated with gene suppression
RNA-mediated interference (RNAi) provides prospective functional analyses through specific silencing of targeted nematode genes Heterodera
hp-SBE IIa lines including IIa 4 showed reduced SBE IIb expression in developing endosperms Hordeum vulgare
stacking of several transgenes avoids homology-dependent gene silencing Oryza sativa
AtNF-YA5 gene overlap results in production of natural antisense transcript (nat)-derived siRNA (natsiRNA) Arabidopsis thaliana
NbCesA8 transcript level is reduced in VIGS-NbCesA8-infected plants Nicotiana benthamiana
OsMADS1 RNAi target region covered C-box domain and part of K-box domain of OsMADS1 Oryza sativa
double-stranded RNA (dsRNA) can have several different origins such as viral replication
transcription termination machinery is involved in protecting genes against silencing
poor efficiency in transcription termination leads to generation of siRNAs from transgenes
InLYP1-silencing lines generated using InLYP1-targeting artificial microRNA Arabidopsis thaliana
Fra a gene expression was down-regulated Fragaria x ananassa
SUVH gene RNAi constructs generated from cDNA fragments
ihpRNA–LeHT3 construct contained 201-bp fragment from 3'-UTR of LeHT3 Solanum lycopersicum
gene downregulation can occur as post-transcriptional gene silencing (PTGS)
transgenes have similarity with prokaryotic genes
siRNA production from transgenes might interfere with splicing
antisense AB3 lines (AB3-8 and AB3-9) show silenced expression of UP9C Nicotiana tabacum
5 mM EGTA in the infiltration media allowed gene silencing to progress to impede HR Nicotiana tabacum
double-stranded RNA (dsRNA) can have several different origins such as convergent transcription
RNA-dependent RNA polymerases (RDRs) convert single stranded RNA
endogene mRNAs are mostly degraded after being targeted by small RNAs (sRNAs)
silencing construct hpRpa1 with a target sequence outside the coding region selectively silenced endogenous Rpa1 Nicotiana tabacum
terminators are involved in protecting genes against silencing
histone modifications functions in monoallelic gene expression
abolishing CG methylation maintenance in a methyltransferase 1 null mutant curiously do not alleviate silencing of tDNA Pro arrays Arabidopsis thaliana
eccDNAs can be source for small interfering RNAs
(AS2, AT1G65620) bodies might play a role in epigenetic repression of the expression of the target gene (ARF3, ETT, AT2G33860) Arabidopsis thaliana
(DDM2, DMT01, DMT1, MET1, MET2, METI, AT5G49160) is responsible for repression of the expression of rDNA in the corresponding perinucleolar compartment Arabidopsis thaliana
splicing affects gene stability
vsiR45349 downregulates abundance of (ATFD1, FD1, AT1G10960) transcript
three RNAi lines (#8, #29 and #71) were obtained for TaNAC77 knockdown Triticum aestivum
predominant fraction of 5S rDNAs is thought to be silent fraction Arabidopsis thaliana
small RNAs (sRNAs) are key for posttranscriptional control of mRNAs
pBI–Fraa3i-infiltrated fruits strongly reduced to less than 40% of the levels Fra a transcript levels
stress-mediated increase in histone acetylation can transiently overrule silenced status of TS–GUS
DNA methylation functions in monoallelic gene expression
artificial eccDNA can express small interfering RNAs
plants can be highly sensitive to gene copy number changes
(CESA6, E112, IXR2, PRC1, AT5G64740) and PRC2 repress transcription Arabidopsis thaliana
knockdown of (PDE226, PDS, PDS3, AT4G14210) expression in fourth and fifth systemically infected leaves at 30 dpi with BMVF13m is less than 20% relative (PDE226, PDS, PDS3, AT4G14210) expression level Zea mays
BMVCP5:HSP70-1 infection in maize plants results in less (AT-HSC70-1, AtHsp70-1, HSC70, HSC70-1, HSP70-1, AT5G02500) expression in upper leaves Zea mays
methylations on H3K9 are associated with transcriptional suppression
RT-PCR was used to assess silencing of myosin genes Nicotiana tabacum
Zingiber officinale BSMV persistence presents opportunity to create a large number of ginger shoots with down-regulated gene expression from inoculation of a single leaf Zingiber officinale
transgenes are subject to transcriptional gene silencing
VIGS using TRV-derived vectors uses Agrobacterium-mediated transfer into host cells
antisense AB3 line AB3-9 shows more effective silencing of UP9D Nicotiana tabacum
(FAS1, FUGU2, NFB2, AT1G65470) targeting to (BP, BP1, KNAT1, AT4G08150) gene suppresses ectopic expression of (BP, BP1, KNAT1, AT4G08150)
VIGS is at least 4 weeks slower than hp RNAi technique Nicotiana benthamiana
post-transcriptional gene silencing (PTGS) of (ABA1, ATABA1, ATZEP, IBS3, LOS6, NPQ2, ZEP, AT5G67030) was successful in TRV2-ZEP-inoculated plants Capsicum annuum
NbIRX8 transcript level is reduced in VIGS-NbIRX8-infected plants Nicotiana benthamiana
siRNAs specifically repress transgene expression
degree of expression affects amount of siRNAs triggered from transgenes
terminators have the biggest impact on amount of siRNAs originating from transgenes
spread of methylation into regulatory regions may cause repression of expression of nearby genes
Tobacco rattle virus (TRV) is basis for most popular VIGS vectors
5 mC is dominant repressor of clustered tDNA expression in Col-0 Arabidopsis thaliana
BSMV (barley stripe mosaic virus)-mediated host-induced gene silencing (HIGS) was used to knock-down Ps (AT-CDA1, CDA1, DESZ, AT2G19570) and Ps CDA2 Puccinia striiformis f. sp. tritici
H3K27me2 in Entamoeba histolytica is enriched at RNAi-mediated silenced genes Entamoeba histolytica
hpRNA gene silencing strategy was used to silence S-adenosyl methyltransferase (SAMT) gene Solanum lycopersicum
15B-2, 15B-5, 15B-6, and 15B-9 progeny of T1 15B line of Kontesa showed highest decrease in expression of Pina and Pinb genes Triticum aestivum
3-5 line was not silenced Triticum aestivum
cultivar 'New Yorker' showed moderately large sectors of photobleaching on silenced leaves Solanum lycopersicum
young seedlings (2 weeks old) show optimum silencing at 21 °C and high humidity Solanum lycopersicum
35S promoter-driven RNAi construct was generated to knock down expression of (AGP6, AT5G14380) and (AGP11, ATAGP11, AT3G01700) Arabidopsis thaliana
P3 and P4 plants show very similar silencing efficiency Arabidopsis thaliana
short interfering RNAs (siRNA) are incorporated into dsRNA-induced silencing complex (RISC)
accessory proteins may participate in nucleation step towards final silencing state of genomic element
UP9C overexpression in antisense orientation results in down-regulated expression of UP9C Nicotiana tabacum
NbCOMT transcript level is reduced in VIGS-NbCOMT-infected plants Nicotiana benthamiana
siR109944 has FBL55 as potential target Oryza sativa
siRNA production from transgenes might have a further negative impact on gene expression
RNAi-MdRNL2 suppresses MdRNL2 expression Malus domestica
Capsicum annuum 'Micropep Yellow' (MY) was subjected to virus-induced gene silencing of zeaxanthin epoxidase (ABA1, ATABA1, ATZEP, IBS3, LOS6, NPQ2, ZEP, AT5G67030) Capsicum annuum
DNA methylation silences repetitive elements
DNA methylation causes stable repression of endogenous genes
RNAi can lead to transcriptional silencing of locus
(KYP, SDG33, SUVH4, AT5G13960) /5/6 and (ATHDA6, AXE1, HDA6, HDAC6, RPD3B, RTS1, SIL1, AT5G63110) deposit H3K9me2
Tobacco rattle virus (TRV) has been established for investigation of enzyme-coding genes and signaling components in tomato Solanum lycopersicum
gene copy number changes result in efficient gene silencing
correlation between silencing efficiency and the resulting phenotypes was not observed within hemizygotes
targeted mRNAs were found to be depleted in nematodes feeding on transgenic plants Meloidogyne sp.
silencing of targeted genes occurred in progeny of feeding nematodes Meloidogyne sp.
NaF6ʹH1 was silenced by VIGS Nicotiana attenuata
siRNA-directed DNA methylation pathway provides insights into chromatin-based pathways for gene silencing
incRNA–PRC2 complex is dispensable for transcriptional silencing
more than fifty VIGS systems are able to target important crop plants
most of tDNAs might be silent Arabidopsis thaliana
miRNA mediate post-transcriptional gene silencing
DNA methylation silences transposons
VIGS method efficiently downregulates genes expressed in glandular trichomes Solanum lycopersicum
non-protein-coding RNA (npcRNA) is component of epigenetic regulation
DNA methylation is associated with repression of expression of nearby genes
transgenes are subject to transcriptional gene silencing
artificial eccDNA can express microRNA
BSMV–VIGS is important practical feature for gene silencing in the Zingiberales Zingiber officinale
disruption of CP synthesis enhances persistence of VIGS Zingiber officinale
ihpRNA–LeHT construct contained 401-bp fragment from conserved coding region of LeHT3 Solanum lycopersicum
overexpression of GFP:PDX1.3 correlates with strong co-suppression of endogenous (ATPDX1.1, PDX1.1, AT2G38230) and (ATPDX1, ATPDX1.3, PDX1, PDX1.3, RSR4, AT5G01410) genes Arabidopsis thaliana
223-bp PCR fragment from ACL1 gene inserted into p1301UbiNos vector forms antisense ACL1 vector
MYC3–RNAi1, MYC3–RNAi2, and MYC3–RNAi3 lines have 30%, 20%, and 10% reduction in (MYC3, AT5G46760) Arabidopsis thaliana
silencing construct expressed in hosts affects expression in parasite
cytosine methylation has role in silencing
increase of histone acetylation in RdDM mutants at specific genomic loci is probably secondary effect of the release of silencing
loss-of-UBP5 function leads to repression of several PcG targets Arabidopsis thaliana
RNAi construct was designed to reduce (ATPMT1, PMT1, AT2G16120) and (ATPMT2, PMT2, AT2G16130) mRNA levels simultaneously Arabidopsis thaliana
N. attenuata plants inoculated with Agrobacterium carrying pTV-NaGSNOR formed NaGSNOR-VIGS plants Nicotiana attenuata
Nicotiana benthamiana (ATFLS2, FLS2, AT5G63580) (Nb ) was depleted by RNAi using hairpin (hp) constructs Nicotiana benthamiana
access to a diversity of regulatory elements is needed to avoid gene silencing
histone methyltransferases (KYP, SDG33, SUVH4, AT5G13960) /5/6 deposit H3K9me2 modifications
NaGSNOR activity was 90% reduced in NaGSNOR-VIGS plants Nicotiana attenuata
virus-induced gene silencing (VIGS) has differences in characteristics compared to systemic TGS by siRNA transmission from inverted repeat transgene
wild-type aspen leaf explants transformed with overexpression vector designed to constitutively express PtdCesA8 resulted in sense co-suppression of both the transgene and the native PtdCesA8 genes Populus tremuloides
plants have evolved a mechanism to differentiate self from non-self
mRNAs may be targeted to RNA-induced silencing complex (RISC)
silencing efficiency can be predicted based on characteristics of parental transgenic plants
TRV vectors that do not encode 16 kDa protein could possibly provide better silencing efficiency
RNA suppression is used for reverse genetics Zea mays
depletion of endogenous immune components with transient hp RNAi depends on stability of the protein Nicotiana benthamiana
PSR1 targets (CUV, EMB3011, FEY, PINP1, PRP16, AT5G13010) Glycine max
(BAP1, AT3G61190) removal leads to widespread reduction in gene expression
CcCBL1 transcript levels decreased by approximately 50% in CcCBL1-RNAi and CcCBL1-RNAi CcCIPK14-OE plants Cajanus cajan
cross-kingdom/species RNAi is paramount for design of SIGS strategies
tomato SlAGO7 represses SlARF3 / 4 Solanum lycopersicum
(FIS1B, AT5G12390) RNAi construct was made in previous study
small interfering RNA (siRNA) can induce transcriptional gene silencing (TGS)
hp-SBE IIb construct essentially completely silenced SBE IIb expression Hordeum vulgare
increased temperature causes increase in RNA silencing
PTGS is therefore at least partially responsible for partitioned silencing
PDC2_E1α was selected for amiRNA silencing Chlamydomonas reinhardtii
modified TRV–GFP vector is as effective as original TRV Nicotiana benthamiana
DNA methylation causes stable repression of transgenes
HT-M RNAi gene construct was used for suppressing all HT-M genes Nicotiana alata
absence of a system for amplification of siRNA within the cells to which it is transmitted would limit degree of transcriptional gene silencing (TGS) spreading
RNAi technology was used to reduce γ-gliadins by up to 80% in bread wheat lines of cv. 'Bobwhite' Triticum aestivum
target-specific RNAi of several different plant-parasitic nematode genes has been achieved through in vitro soaking in solutions of complementary dsRNA plant-parasitic nematodes
gene silencing was overcome by expressing nanosensors in mutant plants deficient in gene silencing (rdr6-11 and (ATSGS3, SGS3, AT5G23570) ) Arabidopsis thaliana
RNAi technology was used to strongly down-regulate all ω-, α-, and γ-gliadins Triticum aestivum
NTM19 gene promoter was used to induce post-meiotic TMBP200 deficiency in microspores Nicotiana tabacum
modified TRV–GFP tested for compatibility with rose seeds Rosa sp.
long non-coding RNAs (ncRNAs) acting in cis are involved in Polycomb group (PcG) recruitment in mammals
TS-GUS activity was increased in idn2-3 fdm1-1 idp2-1 fdm3-2 fdm4-2 fdm5-2 Arabidopsis thaliana
Pc-G complexes repress MEDEA (EMB173, FIS1, MEA, SDG5, AT1G02580) expression Arabidopsis thaliana
rearranged paralogous structure causes transcriptional silencing of the other copy Arabidopsis thaliana
pHANNIBAL construct is capable of suppressing multiple HT-M genes
(GEX3, AT5G16020) antisense construct was only partially down-regulating (GEX3, AT5G16020) Arabidopsis thaliana
(FIS1B, AT5G12390) silencing identification method described in previous study
Hybrids A3-2, A10-1, A14-1, and A16-1 show no detectable HT-M transcript Nicotiana plumbaginifolia; Nicotiana alata
5azaC treatments do not alleviate silencing of tDNA Pro arrays Arabidopsis thaliana
VIGS system allows silencing of multiple genes Solanum lycopersicum
RNAi constructs were causing efficient silencing Hordeum vulgare
high and low AsA plants in GGT-transformed Arabidopsis line suggests occurrence of gene silencing Arabidopsis thaliana
transgene transcript level correlates with (RPS10, uS10m, AT3G22300) silencing Arabidopsis thaliana
all hemizygous transformants of the Rps10.2 line developed only late onset of silencing
double-stranded RNA (dsRNA) is recognized by the cell as aberrant RNA
ingestion of dsRNA by non-feeding J2s was stimulated artificially by adding neurostimulant chemicals Meloidogyne sp.
siRNA transmission would induce transcriptional gene silencing (TGS) in the graft partners
silencing of (PDE226, PDS, PDS3, AT4G14210) in cultivar 'Lichun' was enhanced at low temperatures (15 °C) and low humidity Solanum lycopersicum
concentration of transcripts is lowered by specific targeting and mRNA degradation
onset of (RPS10, uS10m, AT3G22300) silencing depends on genetic and environmental factors
virus-mediated silencing could be effective strategy for delivery of RNAi triggers to feeding nematodes
Agrobacterium-mediated transient assays screened for silencing of GUS reporter gene Solanum lycopersicum
NaHD20 -silenced plants show NaHD20 mRNA levels reduced by 86±7% compared with EV plants Nicotiana attenuata
P1r plants at 10 weeks show lack of (RPS10, uS10m, AT3G22300) silencing in young leaves Arabidopsis thaliana
hemizygous Rps10.1 plants developed either early or late onset
free-living J2s can be silenced by soaking in dsRNA solutions Meloidogyne sp.
low proportion of syncytia producing dsRNA resulted in moderate penetrance of RNAi in nematodes
RNA interference (RNAi) constructs targeting CcCBL1 were introduced in wild-type and CcCIPK14-OE plants Cajanus cajan
various small RNA species mediate RNA silencing
certain mRNAs are processed for RNA interference (RNAi)
normal decay pathway absence leads to activation of RNAi
PcG marks incorporated into canonical and variant histones may guarantee stable maintenance of gene repression Arabidopsis thaliana
six glutelin gene promoters avoid promoter homology-based gene silencing Oryza sativa
hairpin RNA-induced gene silencing technique was employed to suppress total expression of LeEIL1–LeEIL4 Solanum lycopersicum
P2 plants at 16 weeks show decreased (RPS10, uS10m, AT3G22300) transcript levels in all organs Arabidopsis thaliana
promoter methylation is associated with tissue-specific gene repression Arabidopsis thaliana
histone H3K27 trimethylation not enriched at methylated genomic regions suggests that function of (ACG1, ATMSI4, FVE, MSI4, NFC04, NFC4, AT2G19520) in DNA methylation and transcriptional silencing is unlikely to be explained by histone H3K27 trimethylation
transcriptional gene silencing occurs via RNA-directed DNA methylation (RdDM) pathway
OP:SlDCL1IR construct activation by different promoters generated variable silencing strengths Solanum lycopersicum
correlation coefficient (r) = –0.97 indicates strong negative correlation between CP–GFP protein and NtPDS abundance Nicotiana benthamiana
fusion with GFP did not obviously influence silencing efficiency of TRV vector in rose Rosa sp.
InAGN transgenic plants contain antisense (112A-2A, EMB30, GN, GNOM, MIZ2, VAN7, AT1G13980) fragment Arabidopsis thaliana
cPRC1 compacts chromatin animals
hp-SBE IIb construct targets SBE IIb expression Hordeum vulgare
NICTABA-silencing transgenic N. tabacum lines (S111, S351, S411) show reduced NICTABA transcript levels Nicotiana tabacum
P0 plants lack (RPS10, uS10m, AT3G22300) gene silencing Arabidopsis thaliana
homozygous plants have silencing efficiency (RPS10, uS10m, AT3G22300) transcript level decreased to 2–12% relative to the control plants
RNA interference (RNAi) allows phenotype analysis of nematode development and nematode establishment in host
incomplete knock-out of targeted genes in treated nematodes makes difficult interpretation of RNAi phenotypes root-knot nematode
hpXSP10 lines (X1, X2, X3) exhibit complete silencing of GUS reporter gene Solanum lycopersicum
cultivar 'Pearson' showed relatively slight symptoms Solanum lycopersicum
Pro (AGP6, AT5G14380) -driven amiRNA construct was generated to knock down expression of (AGP6, AT5G14380) and (AGP11, ATAGP11, AT3G01700) Arabidopsis thaliana
antisense plants has been used to lower cyFBPase activity
transgene promoter region could be another possible explanation for differences observed in silencing onset in the LD and SD photoperiod
35S:NS-Vitis3 transgenic strawberry line shows dramatically diminished (ATCHS, CHS, TT4, AT5G13930) mRNA levels in fully expanded leaves Fragaria × ananassa
nickel ions can induce transgene silencing
variability in silencing efficiency was observed between progeny collected from individual plants root-knot nematode
barley lines with low expression of SBE IIa or SBE IIb were generated through RNA-mediated silencing technology Hordeum vulgare
transgenic plant with GFP-partitioned phenotype possessed characteristic pattern of GFP-expressing and silenced regions Nicotiana benthamiana
active initiation and establishment of silencing is affected by temperature
radial wave of silencing suggested mobile signals initially reached mid and lateral veins through phloem flow Nicotiana tabacum
partitioned-silenced regions definitely possess mobile signals for systemic transmission of silencing Nicotiana tabacum
miR168a-1 is cosuppression line of (MIR168, MIR168B, AT5G45307) Solanum lycopersicum
Chlamydomonas reinhardtii CC-1618 mutants with decreased expression of PDC2_E1α were generated using artificial microRNA Chlamydomonas reinhardtii
modification of TRV–GFP does not jeopardize function of TRV in gene silencing Nicotiana benthamiana
PDS-positive plant is defined as plant exhibiting typical photobleached phenotype and substantially decreased RhPDS transcript Rosa sp.
SlCYP707A2 is markedly downregulated to 18% of control in SlCYP707A2-RNAi-treated fruits Solanum lycopersicum
cytosine DNA methylation directed by lmiRNAs results in transcriptional gene silencing Arabidopsis thaliana
(ATSGS3, SGS3, AT5G23570) shows elevated expression in central cell Arabidopsis thaliana
upregulated genes in amiTEK show significant overlap with genes silenced by (DDM2, DMT01, DMT1, MET1, MET2, METI, AT5G49160) and (ATHDA6, AXE1, HDA6, HDAC6, RPD3B, RTS1, SIL1, AT5G63110) Arabidopsis thaliana
pML-Hyg-35S promoter:Amikn126 construct was designed to knock down AtBP and AtKNAT2/AtKNAT6 expression Arabidopsis thaliana
TRV2::GFP is constructed as control Nicotiana benthamiana
both transcripts from (AT2G41590) (AT2G41600) and (OXR6, AT4G34070) (AT4G34071) pairs were under regulation of nat-siRNAs Arabidopsis thaliana
amiRNA of 22 nt in length is sufficient for triggering transitivity
cultivar selection is needed for adequate responses for silencing studies Solanum lycopersicum
Southern analysis demonstrated presence of putatively silenced sexual allele Paspalum simplex
NbVPE1a and NbVPE1b silencing was produced using two DNA fragments from NbVPE1a and NbVPE1b coding regions Nicotiana benthamiana
P3 plants at 13 weeks show decreased (RPS10, uS10m, AT3G22300) mRNA level in young leaves Arabidopsis thaliana
P4 plants at 16 weeks show normal (RPS10, uS10m, AT3G22300) mRNA level in rosette leaves Arabidopsis thaliana
selected photoperiod was the crucial factor determining whether or not late onset of silencing occurred
silenced genes can undergo spontaneous and developmentally regulated reactivation by a process called resetting
two plants agroinfiltrated with TRV::TNC display <50% reduction in Mi-tnc transcripts Nicotiana benthamiana; Meloidogyne incognita
silencing machinery is triggered by abundance of sexual mRNA target sufficiently high Paspalum simplex
DNA methylation correlates with siRNAs Arabidopsis thaliana
concerted process of silencing is disrupted in ddm1- mutant plants Arabidopsis thaliana
hpRNAi produces variable set of siRNAs
Tobacco rattle virus (TRV) vector was used to silence NbNTF6 Nicotiana benthamiana
silencing (PDE226, PDS, PDS3, AT4G14210) is unsuitable as silencing reporter Solanum lycopersicum
sequences determined from this study could be used in creation of RNA interference silencing mutants Medicago truncatula; Lotus japonicus
TRV (Tobacco Rattle Virus) can produce RNAi triggers in feeding cells
RNAi experiments yielded varying levels of silencing efficiency Triticum aestivum
silencing efficiency is lower at 25 °C growth temperature Lycopersicon esculentum
vector containing fragment of Lc in tandem with fragments of target genes was used as standard system Solanum lycopersicum
NbrbohA/B-silenced Nicotiana benthamiana shows decreased transcript of NbrbohA transcript Nicotiana benthamiana
soaking strategy for dsRNA-mediated gene silencing is limited to functional analysis of genes involved in early steps of parasitism Meloidogyne sp.
transgenic lines (AtTK1b, TK1b, TK2, AT5G23070) and TK4 were selected for further analysis Actinidia chinensis
reversion observed in P1r plants does not result from complete inactivation of transgene transcription Arabidopsis thaliana
increase in (RPS10, uS10m, AT3G22300) transcript abundance is correlated with time when first P3 plants present onset of late silencing Arabidopsis thaliana
phenotype variety of hemizygous P2, P3, and P4 plants cannot be attributed to silencing efficiency Arabidopsis thaliana
P2 plants show very similar silencing efficiency Arabidopsis thaliana
environmental conditions resulted in a shift in onset of silencing Nicotiana plumbaginifolia
TRV is able to mediate delivery of RNAi triggers to feeding nematodes
hypermethylation of (FLD, RSI1, AT3G10390) promoter would result in lower (FLD, RSI1, AT3G10390) expression Arabidopsis thaliana
RNAi technology was used to reduce content of α-gliadins in lines of bread wheat cv. Florida Triticum aestivum
glutelin promoters avoid promoter homology-based gene silencing
Manduca sexta oral secretion (OS)-inducible PME was silenced by RNA interference (RNAi)-mediated gene silencing Nicotiana attenuata
P1r phenotype suggests reversion from silenced state Arabidopsis thaliana
P1r plants show higher transgene mRNA accumulation Arabidopsis thaliana
inactivation of silencing was limited only to homozygous plants
silencing in progeny of feeding nematodes showed high variability in gene silencing efficiency root-knot nematode
variability in efficiency of virus propagation could result in variations in dsRNA or siRNA accumulation between root systems
study of GFP-partitioned phenotype delineates features of specific silencing Nicotiana benthamiana
C. reinhardtii recombinant lines with decreased expression of PDC2_E1α were generated using artificial microRNA (amiRNA) technology Chlamydomonas reinhardtii
compacted chromatin was shown to promote PRC2 activity
computational analyses of sequences of Polycomb group (PcG) recruitment sites in mammals failed to identify common DNA sequence motifs involved in Polycomb group (PcG) targeting
silencing (PDE226, PDS, PDS3, AT4G14210) has been reported by two other research teams Solanum lycopersicum
silencing of TiERF1 gene presumably resulted in no detectable TiERF1 expression in T3 plants of E314-3-3 Triticum aestivum
constructions under the control of the native promoter for the (AGP6, AT5G14380) gene were used for RNAi-mediated silencing of (AGP6, AT5G14380) and (AGP11, ATAGP11, AT3G01700) Arabidopsis thaliana
P1, P3, and P4 phenotypes likely result from silencing of (RPS10, uS10m, AT3G22300) gene Arabidopsis thaliana
phenotypic variety resulted from at least two events, separated in time, of the onset of (RPS10, uS10m, AT3G22300) silencing
phenotypes were associated with timing of the onset of silencing
inactivation of silencing is induced by very low (RPS10, uS10m, AT3G22300) transcript level found only in homozygotes
Tobacco rattle virus (TRV) mediates RKN gene silencing
viral vectors can be used to mediate dsRNA production in plants
VIGS method was used to produce single-silenced and dual-silenced N. benthamiana plants Nicotiana benthamiana
green hotspots were seen adjacent to silencing region but unable to penetrate it
TRV–GFP vector tested to induce gene silencing in Arabidopsis thaliana plants Arabidopsis thaliana
TRV–GFP could spread and silence endogenous genes Rosa sp.
miRNAs modulate target gene expression by mRNA cleavage
VPE genes have been specifically silenced via VIGS (virus-induced gene silencing) Nicotiana benthamiana
fully silenced plants did not express GFP mRNAs
PaWOX8/9 RNAi lines down-regulate PaWOX8/9 transcript level Picea abies
silencing (PDE226, PDS, PDS3, AT4G14210) gives clear phenotype Solanum lycopersicum
non-expression of the transgenic plants presumably results from silencing at the transcript level of the introduced genes Triticum aestivum
RNA interference construct using LeEIL2 fragments employed to obtain transgenic tomato plants with severely suppressed LeEIL genes Solanum lycopersicum
NbrbohA-silenced Nicotiana benthamiana shows decreased transcript of NbrbohA transcript Nicotiana benthamiana
early onset responsible for the appearance of the P2 phenotype may result from higher silencing competence of the transgene loci present in the Rps10.1 line
silencing inactivation occurs even in tissues where silencing onset has already occurred, resulting in severe morphological abnormalities
production of targeted transcripts is often restored a few days after soaking Meloidogyne sp.
initiation or arrest of silencing might be dependent on region-specific genes
transmission rate of silencing was higher and faster in younger plants Nicotiana tabacum
endogenous NtPDS transcript greatly decreased in TRV-NtPDS-infected plants Nicotiana benthamiana
lmiRNA-directed DNA methylation results in transcriptional gene silencing
cultivar 'Pearson' had high infection efficiency Solanum lycopersicum
endogenous (ATCHS, CHS, TT4, AT5G13930) was used as reporter for silencing Petunia hybrida
GFP fluorescence did not drop to zero in the presence of matching RNAi construct Hordeum vulgare
J47/1 transgenic strawberry line shows decreased (ATCHS, CHS, TT4, AT5G13930) expression in fully expanded leaves Fragaria × ananassa
photobleaching indicates efficiency of virus-induced NbVPE-silencing Nicotiana benthamiana
BC 2 line showed significantly reduced SBE IIa and SBE IIb expression Hordeum vulgare
FRET nanosensors are subject to gene silencing in wild-type Arabidopsis plants Arabidopsis thaliana
amiRNA can silence non-homologous multiple genes Oryza sativa
antisense line 2092 shows decreased expression of (ATVAMP711, VAMP711, AT4G32150) expression Arabidopsis thaliana
antisense line 2092 shows decreased expression of (ATVAMP714, VAMP714, AT5G22360) expression Arabidopsis thaliana
normal level of accumulation of SBE IIb transcripts in SBE IIa targeted lines indicates cross suppression was not due to RNAi mechanism directly Triticum aestivum
estradiol-inducible artificial miRNA against (ADL1, ADL1A, AG68, DL1, DRP1A, RSW9, AT5G42080) (ADL1C, ADL5, DL1C, DRP1C, AT1G14830) and (ADL1E, ADL4, ADLP2, DL1E, DRP1E, EDR3, AT3G60190) targets (ADL1, ADL1A, AG68, DL1, DRP1A, RSW9, AT5G42080) (ADL1C, ADL5, DL1C, DRP1C, AT1G14830) and DRP1E—the most abundant isoforms Arabidopsis thaliana
35S::amiTEKa targets (AHL16, TEK, AT2G42940) coding region Arabidopsis thaliana
(TAS2, AT2G39681) is endogenous target of (MIR173, AT3G23125)
EXORIBONUCLEASE 4 (AIN1, ATXRN4, EIN5, XRN4, AT1G54490) is suppressor of gene silencing Arabidopsis thaliana
silencing efficiency is lower at 20 °C growth temperature Lycopersicon esculentum
silencing Leaf colour (Lc) expression provides convenient silencing reporter Lycopersicon esculentum
temperature optimum for silencing is relatively sharp Solanum lycopersicum
RNAi construct targeting HvDRF1 exhibited significant phenotypic effect Hordeum vulgare
hemizygotes presented quite diverse morphology (P2, P3, and P4)
homozygous P1 plants ∼17% were able to switch off silencing in late vegetative growth stage
loss of purple pigmentation is used as reporter for silencing of floral genes Petunia
RNAi construct targeting HvDREB1 exhibited significant phenotypic effect Hordeum vulgare
successful onset of silencing not only depends on the mRNA level exceeding the threshold but it also requires that this favourable condition is maintained for longer time periods
reversion observed in the case of the P1r plants is apparently not associated with cell proliferation
virus-inoculated plants can produce dsRNA and siRNA silencing triggers
virus-induced gene silencing (VIGS) is triggered by viral vectors that deliver dsRNA fragments homologous to targeted plant genes
two plants agroinfiltrated with TRV::TNC display 80–90% reduction in Mi-tnc transcripts Nicotiana benthamiana; Meloidogyne incognita
hpRNA construct contains inverted repeat of XSP10 and GUS sequences Solanum lycopersicum
virus-induced gene-silencing system was used to generate empty vector (EV) and NaBAK1-silenced plants Nicotiana attenuata
antisense approach provides set of plants with different degrees of inhibition
J47/2 transgenic strawberry line shows decreased (ATCHS, CHS, TT4, AT5G13930) expression in fully expanded leaves Fragaria × ananassa
(ATCHS, CHS, TT4, AT5G13930) introduction has caused silencing of transferred gene and endogenous homologue
aberrant RNA is processed by Dicer RNase
hpPbCCT5 expression caused PbCCT5 silencing
PbCCT5 hairpin construct is driven by 35S promoter Pyrus betulaefolia
single guide RNA (sgRNA) silences up to 16 transcription activator-like effectors (TALEs) in Xanthomonas strain Xanthomonas infecting rice; Xanthomonas infecting citrus; Xanthomonas infecting Brassica; Xanthomonas infecting cassava
amiR-MpBES1 is designed to target MpBES1 Marchantia polymorpha
secondary siRNAs can target endogenous mRNA
RNA interference (RNAi) often suffers from unstable gene suppression
NbAG expression is significantly reduced in TRV2-NbAG treated plants Nicotiana benthamiana
RNAi:: MtNoa1/ (ATNOA1, ATNOS1, NOA1, NOS1, RIF1, SVR10, AT3G47450) construct decreased MtNoa1/Rif1 gene expression by 90% Medicago truncatula
multiple gene silencing by RNA interference should be explored to strongly reduce expression of both (GGT1, AT4G39640) and (GGT2, AT4G39650) isoforms Arabidopsis thaliana
phenotype of plants in which genes were silenced is highly dependent on cultivar and growing conditions Petunia
higher frequency and greater severity of gene silencing in homozygous transformants have been reported homozygous transformants
short interfering RNAs (siRNA) guide degradation of homologous transcripts
N. benthamiana as host showed similar silencing efficiency in all galls induced by feeding RKNs Nicotiana benthamiana; root-knot nematode
Single guide RNA multiplexing has been reported for up to 10 different targets at the same time
high level of conservation of promoter sequences enabled extension of knockdown analysis to Xanthomonas tale repertoires in four other crop pathogens Xanthomonas
VIGS assays is used to silence GoPGS homeologs Gossypium hirsutum
strain COR308 was employed for further analysis and optimization of the transient RNAi assay Agrobacterium tumefaciens; Triticum aestivum
fungal double-stranded RNAs in wheat cells subsequently acted as substrates for plant nucleases catalyzing their cleavage into corresponding siRNA molecules Triticum aestivum
Csi-miR3954 is 22-nt miRNA Citrus sinensis
Cs1g09635 3′D6(−) might function by targeting NAC transcription factors Citrus sinensis
partitioned-silenced regions (PS-1 to PS-3) did not express GFP mRNA
percentage of PDS-positive plants similar between TRV-RhPDS and TRV–GFP-RhPDS plants Rosa sp.
TRV–GFP vector could be used widely in multiple plants
HvABCD1 and HvABCD2 expression was reduced simultaneously using RNAi approach Hordeum vulgare
MEDEA (EMB173, FIS1, MEA, SDG5, AT1G02580) is silenced during vegetative development Arabidopsis thaliana
repressive H3K27 methylation causes silencing of MEDEA (EMB173, FIS1, MEA, SDG5, AT1G02580) expression Arabidopsis thaliana
Agrobacterium tumefaciens-mediated PITGS can be used as reverse-genetics tool to discover gene function in rust fungi Puccinia triticina
potential silencing effects by fungal-specific siRNA molecules on transcripts in the wheat genome cannot be completely ruled out Triticum aestivum
siRNA molecules were generated in host cells Triticum aestivum
i-ir (PDE226, PDS, PDS3, AT4G14210) line expresses RNAi-mediated gene silencing construct Nicotiana attenuata
PME amiRNA transgenic cells showed substantial reduction in PME transcript levels Penium
Cs1g09635 3′D6(−) targets Cs7g22560 Citrus sinensis
Transgenic plants with dsRNA targeting the MpFPPS1 gene obtained and analyzed by RT-PCR Nicotiana tabacum; Myzus persicae