| 2-oxoglutarate |
is partially used for |
fatty acid biosynthesis |
Glycine max |
| malic enzyme and pyruvate dehydrogenase activities in plastids |
produce |
one NADPH, one NADH, and one acetyl-CoA molecule |
|
| acyl-ACP pools |
were examined to assess |
whether additional supply of carbon from malate could trigger a new bottleneck at the point of fatty acid synthesis |
Glycine max |
| C14 (ACP, ACP1, AtACP1, AT3G05020) |
is |
the longest acyl-ACP without a chain-specific thioesterase in soybean |
Glycine max |
| NADPH-producing malic enzyme (ME) and pyruvate dehydrogenase complex (PDH) |
deliver stoichiometric amounts of |
acetyl-CoA |
Glycine max |
| acyl-ACPs |
are located to a lesser extent in |
mitochondria of plants |
|
| malic enzyme activity |
produces |
lipid carbon from glutamine |
Glycine max |
| absence of an HFA-specific TAG lipase |
reduced carbon availability leads to |
reduced FA synthesis |
|
| AtME4 transgenic allele |
provides |
balanced supply of carbon and reducing equivalents |
Glycine max |
| Tsu-0 allele |
promotes |
(ACC2, AT1G36180) function |
Arabidopsis thaliana |
| rate of FA synthesis in developing (cL37, PSRP5, AT3G56910) |
is reduced by 45% compared with |
(FAE1, KCS18, AT4G34520) parent at 4 DAS |
|
| fatty acid biosynthesis |
utilizes |
reducing equivalents |
|
| acyl-acyl carrier protein (ACPs) levels |
were quantified over |
development |
Glycine max |
| R6 stage of development |
showed larger accumulation of |
C14 (ACP, ACP1, AtACP1, AT3G05020) |
Glycine max |
| fatty acid biosynthesis |
appeared at |
28 dpi |
|
| increasing malic enzyme activity |
may push |
carbon into fatty acid synthesis |
Glycine max |
| flux studies of wild-type soybean seed metabolism |
indicated that |
malic enzyme, not (PCK2, PEPCK, AT5G65690) supported fatty acid biosynthesis |
Glycine max |
| (ACP, ACP1, AtACP1, AT3G05020) levels |
did not differ significantly between |
events |
Glycine max |
| fatty acids (FAs) |
are exclusively synthesized in |
stroma of chloroplasts |
|
| (ACCD, ATCG00500) gene in Arabidopsis |
encodes |
one subunit of the chloroplast-localized heteromeric acetyl-coenzyme A carboxylase (ACCase) |
Arabidopsis thaliana |
| fatty acid biosynthesis |
is |
essential |
|
| fatty acid concentrations in transgenic tobacco and tomato plants expressing ShMKS2 |
either did not decrease or decreased only slightly |
|
Nicotiana tabacum; Solanum lycopersicum |
| CARB1 gene |
catalyzes |
first committing step in FA synthesis |
Chlamydomonas reinhardtii |
| levels of ATP and FFA in (ATVPS34, PI3K, VPS34, AT1G60490) KD |
suggest that the direction of carbon flux is toward |
FFA biosynthesis |
Chlamydomonas reinhardtii |
| common herbicides |
target |
novel enzyme |
Poaceae |
| heteromeric acetyl-coenzyme A carboxylase |
can function instead of |
functional (ACC2, AT1G36180) protein |
Arabidopsis thaliana |
| transgenic plants constitutively expressing ShMKS2 with or without ShMKS1 |
had trace amounts of |
myristic acid |
Arabidopsis thaliana; Nicotiana tabacum; Solanum lycopersicum |
| expressing a short-chain thioesterase in the alga Phaeodactylum tricornutum |
led to simultaneous increase in total fatty acids and decrease in |
growth rate |
Phaeodactylum tricornutum |
| (AtLEC1, EMB 212, EMB212, LEC1, NF-YB9, AT1G21970) |
plays a significant role in promoting |
fatty acid biosynthetic genes associated with seed maturation |
|
| Solanum habrochaites methylketone synthase2 (ShMKS2) |
hydrolyzes |
β-ketoacyl acyl-carrier protein |
Solanum habrochaites |
| difference in rates of methylketone synthesis between Arabidopsis and tobacco |
might be a result of |
tobacco seedlings having a higher fatty acid flux rate |
Arabidopsis thaliana; Nicotiana tabacum |
| mutant line |
accumulates TAG by FA biosynthesis bypassing |
TCA cycle |
Chlamydomonas reinhardtii |
| acetyl-coenzyme A carboxylase (ACCase) |
catalyzes conversion of |
acetyl-CoA to malonyl-CoA |
Arabidopsis thaliana |
| (ATVPS34, PI3K, VPS34, AT1G60490) knockdown line under nitrogen starvation |
initiates |
de novo fatty acid biosynthesis |
Chlamydomonas reinhardtii |
| (BADC3, BLP2, AT3G15690) mutant |
shows smaller increase in |
seed fatty acid content relative to wild type |
Arabidopsis thaliana |
| (BADC1, BLP3, AT3G56130) (BADC2, BLP1, AT1G52670) double mutant seeds |
show |
fatty acid increases of 15% to 30% |
Arabidopsis thaliana |
| de novo fatty acid synthesis |
occurs inside |
chloroplasts |
Nannochloropsis oceanica |
| conversion of carbon sources into acetyl-CoA |
differs in |
plastids from different plant tissues and species |
|
| RubisCO bypass reaction |
produces |
TPS |
|
| expression patterns of Chr4.2307.KCS, Chr4.2308.KCS, and Chr4.2311.KCS |
suggested |
these three genes are critically important in lipid metabolism and might be mainly responsible for regulating nervonic acid biosynthesis in Acer truncatum |
Acer truncatum |
| ShMKS2 |
is |
thioesterase |
|
| fatty acid biosynthesis-related genes |
are highly expressed primarily at |
21 days after flowering (DAF) |
Brassica napus |
| overexpression of ω3 fatty acid desaturases (AtFAD3, FAD3, AT2G29980) and (AtFAD7, FAD7, FADD, AT3G11170) |
increases |
α-linolenic acid levels (18:3) |
Nicotiana tabacum |
| low levels of myristic acid |
were found in |
trichomes of wild tomato (accession no. PI126449) |
Solanum habrochaites |
| phenotypes observed in ShMKS2-expressing plants |
are consistent with |
interference with fatty acid biosynthesis |
Arabidopsis thaliana; Nicotiana tabacum; Solanum lycopersicum |
| EcCPS expression |
results in higher accumulation of CPA than |
plant (ABC33, ATCPS1, CPS, CPS1, GA1, AT4G02780) expression |
|
| down-regulation of key TCA cycle genes, including (PCK1, PEPCK, AT4G37870) |
results in TAG accumulation by FA biosynthesis bypassing |
TCA cycle |
Chlamydomonas reinhardtii |
| DG2 and PD1a |
recovered FA synthesis by 55% and 56% compared with |
(cL37, PSRP5, AT3G56910) |
|
| auxotrophic mutants unable to produce biotin |
result in |
embryo lethality |
Arabidopsis thaliana |
| genes in black module |
were positively related to |
nervonic acid biosynthesis (P < 0.09) |
Acer truncatum |
| STEAROYL-ACYL CARRIER PROTEIN Δ9-DESATURASE (SAD) |
initiates biosynthesis of |
polyunsaturated fatty acids (PUFAs) |
|
| not all fatty acids |
were equally affected |
|
Arabidopsis thaliana; Nicotiana tabacum; Solanum lycopersicum |
| ShMKS2 |
is also capable of hydrolyzing |
fully reduced myristoyl-acyl-carrier protein |
|
| majority of FA biosynthesis genes |
were upregulated in |
T411–N compared to WT–N |
Chlamydomonas reinhardtii |
| phosphatidylcholine (PC) and phosphatidylethanolamine (PE) |
are important for producing |
20:5 that is incorporated into chloroplast membrane |
Nannochloropsis oceanica |
| successful engineering of tomato trichomal methylketone production |
might require increases in metabolic flux toward |
fatty acid biosynthetic pathway in trichomes |
Solanum lycopersicum |
| Arabidopsis acyl-acyl-carrier protein thioesterase (FATB, AT1G08510) mutant |
displays |
growth retardation, variegated leaves, and reduced fertility |
Arabidopsis thaliana |
| similar expression profiles of auxin biosynthesis-related genes and Type I or II fatty acid synthesis-related genes |
indicate |
important roles of auxin in fatty acid (FA) biosynthesis |
Arabidopsis thaliana |
| fatty acid concentrations in transgenic Arabidopsis plants expressing ShMKS2 |
increased in general |
|
Arabidopsis thaliana |
| acetyl-CoA carboxylase and fatty acid synthase |
are critical for |
fatty acid biosynthesis |
Chlamydomonas reinhardtii |
| three other subunits of acetyl-coenzyme A carboxylase (ACCase) |
are encoded by |
nuclear genes |
Arabidopsis thaliana |
| constitutive expression of ShMKS2, either with or without ShMKS1, in transgenic cultivated tomato |
led to |
myristic acid accumulation in planta |
Solanum lycopersicum |
| 34 genes predicted to affect elongation cycle |
include |
three genes encoding (ATTSC13, CER10, ECR, GLH6, TSC13, AT3G55360) two encoding HCD, one encoding KCR, and 28 encoding KCS |
Acer truncatum |
| Nossen allele |
decreases |
(ACC2, AT1G36180) function |
Arabidopsis thaliana |
| S. foetida |
accumulates sterculic acid equally at |
all sn-positions |
Sterculia foetida |
| ER-localized phosphatidylcholine (PC) |
has been proposed to contribute to |
20:5 biosynthesis |
Nannochloropsis oceanica |
| heterologous expression of E. coli (ABC33, ATCPS1, CPS, CPS1, GA1, AT4G02780) |
resulted in accumulation of approximately |
5.0% cyclopropane fatty acid (CPA) in primary transformants |
Arabidopsis thaliana |
| fatty acid biosynthesis |
is conserved between |
Brassica napus and Arabidopsis thaliana |
Brassica napus; Arabidopsis thaliana |
| different regulation of auxin and jasmonic acid (JA) in fatty acid (FA) biosynthesis |
is indicated by |
dissimilar altered fatty acid component patterns between axr2-1 and axr1-3 or (COI1, AT2G39940) mutants |
Arabidopsis thaliana |
| pyruvate |
is able to support |
fatty acid biosynthesis |
|
| malate |
is able to support |
fatty acid biosynthesis |
|
| PCR-positive transformants |
revealed |
elevated levels of 16:1n7 compared with wild type |
Phaeodactylum tricornutum |
| repression or loss of (NTT, WIP2, AT3G57670) activity |
results in dramatically decreased |
lipids in oil-storing Arabidopsis seeds |
Arabidopsis thaliana |
| reduced oil content (OC) |
may be due to |
impairment of fatty acid (FA) synthesis |
|
| distinct plastidial transport processes |
are required |
fatty acid biosynthesis |
|
| fatty acid biosynthesis |
utilizes |
ATP |
|
| expression level trends of Chr4.2308.KCS, Chr4.2307.KCS, and Chr4.2311.KCS |
were generally consistent with |
accumulation of nervonic acid (NA) |
Acer truncatum |
| PbGPAT5 (accession number 103942961) |
transcript levels are significantly higher in |
rainfall-treated russet and semi-russet fruit than in control fruit |
Pyrus × bretschneideri |
| FADX |
catalyzes conversion of linoleic acid to |
α-ESA (alpha-eleostearic acid) |
|
| efforts to engineer seeds of crop and model plant species |
have met with only modest success |
production of unusual fatty acids |
|
| acyl groups attached to (ACP, ACP1, AtACP1, AT3G05020) |
can be characterized via |
mass spectrometry |
|
| (GLC, AT1G65450) 6-P |
is imported by |
(GPT, AT2G41490) |
|
| fatty acid biosynthesis |
is completed via activities of |
fatty acid synthases, elongases, desaturases, and carboxylases |
|
| Acer truncatum genome |
has |
34 genes predicted to affect four reactions of elongation cycle |
Acer truncatum |
| MYB transcription factors (TFs) |
have been found to play important roles in |
VLCFA biosynthesis |
|
| metabolic engineering |
enables expression of |
high levels of new fatty acids |
|
| AM fungi (AMF) |
lack |
genes encoding fatty acid synthase I subunits |
Glomeromycotina |
| PDH activity |
is low in |
leaves |
|
| ShMKS2 |
utilizes |
fatty acid biosynthetic intermediates and fatty acids |
|
| ER-localized phosphatidylethanolamine (PE) |
has been proposed to contribute to |
20:5 biosynthesis |
Nannochloropsis oceanica |
| seed-specific co-expression of E. coli cyclopropane synthase with lysophosphatidic acid acyltransferase (LPAT) from Sterculia foetida seeds |
resulted in |
small increases in cyclopropane fatty acid accumulation |
Arabidopsis thaliana |
| protein conformations generated by (ACP, ACP1, AtACP1, AT3G05020) interactions |
influence |
chain flipping and thus catalytic outcomes |
Escherichia coli |
| acetate |
is assumed to be |
precursor for acetyl-CoA |
|
| enzymes |
mediate |
fatty acid biosynthesis |
|
| Brassica napus |
is subject of study for |
fatty acid metabolism |
Brassica napus |
| FA biosynthesis |
is divided into |
cytosolic glycolytic pathway, transport of carbon sources into plastids, starch metabolism, plastidic glycolytic pathway, plastidic OPPP, and FA synthesis pathway |
|
| conjugated fatty acid accumulation in Arabidopsis and soybean seeds engineered to express FAD2-related fatty acid conjugases |
previously noted for |
defective flux of acyl chains from PC to TAG |
Arabidopsis thaliana; Glycine max |
| (AtFAD2, FAD2, AT3G12120) and FADX |
are involved in |
total tung seed oil and α-ESA production |
Vernicia fordii |
| engineered seeds of crop and model plant species |
produce |
hydroxy and conjugated fatty acids |
|
| acyltransferases |
specialization of and possible arrangement in |
metabolons |
|
| (ACP, ACP1, AtACP1, AT3G05020) |
can interact in unique ways with |
wide variety of partners |
Escherichia coli |
| plants |
express |
long-chain polyunsaturated fatty acids |
|
| FAs |
is |
plastid localised fatty acid synthase |
|
| different enzymes specialized for fatty acid pathways |
requires determination of relative contributions in native species |
metabolic engineering of unusual fatty acids |
|
| Crambe seeds compared to other oilseeds including safflower (Carthamus tictorius) seeds |
are particularly effective at producing high levels of erucic acid due to |
low PDCT activity that effectively precludes exchange of fatty acids between DAG and PC |
Crambe abyssinica; Carthamus tictorius |
| genes encoding cytochrome P450 monooxygenase oxidoreductase (PbP45086A8, accession number 103957382) and fatty acid elongase (PbP45086B1, accession number 103943216) |
are significantly upregulated in |
semi-russet CG fruit compared to controls |
Pyrus × bretschneideri |
| metabolic bottleneck for flux of unusual fatty acids into TAG following their synthesis on PC |
is a major limitation for |
producing industrial fatty acids in engineered oilseeds |
|
| C16:0, C18:0 and C18:1 fatty acids |
are synthesized in |
plastids |
|
| α-ESA |
is produced and stored in |
tung tree seed endosperm |
|
| specialized castor bean PDCT activity |
is necessary for |
high level hydroxy fatty acid accumulation |
Arabidopsis thaliana |
| bryophyte metabolism |
exhibits |
very long-chain polyunsaturated fatty acids (vlcPFAs) |
|
| chromoplasts |
possess |
entire metabolic equipment for synthesis of 3-oxoacyl-ACP |
Solanum lycopersicum |
| unusual fatty acids |
are subject of |
biosynthesis studies |
|
| acetate |
is able to support |
fatty acid biosynthesis |
|
| acetate |
is activated inside |
chloroplast |
|
| OLEATE DESATURASE 1 (AtOLE1) |
expression upregulates |
fatty-acid biosynthesis |
Nicotiana benthamiana |
| heteromeric acetyl-CoA carboxylase (ACCase) and fatty acid synthase (FAS) |
results in |
acyl-ACP moieties that are 16 or 18 carbons long |
|
| (AtFaTA, FaTA, FATA1, AT3G25110) /B thioesterases |
produce |
free fatty acids |
|
| fatty acids |
synthesized in leucoplasts using |
acetate, pyruvate, and malate as substrates |
|
| Carboxyltransferase Interactor 1 (CTI1, AT1G42960) |
is an interactor of |
α-carboxyltransferase subunit of acetyl-CoA carboxylase (ACCase) |
Arabidopsis thaliana |
| 3-oxoacyl-ACP |
is |
precursor of fatty acids |
Solanum lycopersicum |
| WRINKLED 1 (ASML1, ATWRI1, WRI, WRI1, AT3G54320) |
expression upregulates |
fatty-acid biosynthesis |
Nicotiana benthamiana |
| 3-oxoacyl-ACP |
is |
precursor of fatty acids |
|
| fatty acid (FA) biosynthesis |
is important for increasing |
oil and fatty acid (FA) content |
Zea mays mays |
| oilseeds |
provide platform for production of |
high-value fatty acids |
|
| Ado-Met |
provides |
methylene group used in biosynthesis of fatty acids |
|
| palmitic acid, stearic acid, and linoleic acid |
showed different percentages under rich light and canopy light |
tung tree seed oil composition |
|
| betaine lipid diacylglyceryl-N,N,N-trimethylhomoserine in pdh lines |
showed most noticeable alterations in |
increased proportion of palmitic acid 16:0 and decreased proportion of linoleic acid 18:2 Δ9,12 |
Chlamydomonas reinhardtii |
| CaMV35S:FAE1 transgenic Arabidopsis plants |
can accumulate |
high levels (>30%) of very-long-chain fatty acids (VLCFAs) in leaf membrane lipids |
Arabidopsis thaliana |
| peroxygenase encoded by SGN-U567002 |
is involved in |
formation of 9,12,13-trihydroxy-10(E)-octadecenoic acid |
Solanum lycopersicum |
| light |
affects |
fatty acid synthesis |
|
| acetyl-CoA |
is converted to |
malonyl-CoA precursors by acetyl-CoA carboxylase (ACCase) |
Haematococcus pluvialis |
| TET |
strongly represses accumulation of |
9,12,13-trihydroxy-10(E)-octadecenoic acid |
Solanum lycopersicum |
| malonyl-CoA:ACP transacylase (EMB3147, MCAMT, MCAT, AT2G30200) reaction |
forms |
malonyl-ACP |
Haematococcus pluvialis |
| expression levels of ACCase, (EMB3147, MCAMT, MCAT, AT2G30200) KAS II, III, HD, and SACPD |
were similar between |
haematocysts and macrozooids |
Haematococcus pluvialis |
| mitochondrial activity in SUL |
is possibly directed into |
mitochondrial fatty acid formation |
Solanum tuberosum |
| (ACBP4, AtACBP4, AT3G05420) and (ACBP5, AtACBP5, AT5G27630) |
could participate in |
transfer of oleoyl-CoA esters to the endoplasmic reticulum (ER) from the chloroplasts |
Arabidopsis thaliana |
| H. pluvialis |
actively synthesized |
fatty acids de novo—mainly oleic, palmitic, and linoleic acids |
Haematococcus pluvialis |
| NADPH-producing malic enzyme (ME) and pyruvate dehydrogenase complex (PDH) |
deliver stoichiometric amounts of |
NADH |
Glycine max |
| short time frame during which transport of the essential co-factor biotin across the embryo epidermis is catalysed by (ATSUC5, SUC5, AT1G71890) |
is |
sufficient for proper fatty acid synthesis |
Arabidopsis thaliana |
| levels of KAR and KAS I transcripts |
were increased to the greatest degree (2.75- and 3.5-fold, respectively) in response to |
conditions of high light |
Haematococcus pluvialis |
| acetyl CoA carboxylase |
catalyzes |
fatty acid biosynthesis |
|
| malic enzyme |
is the source of |
pyruvate |
Glycine max |
| microalgal lipid metabolism |
involves |
carbon allocation to fatty acid (FA) synthesis |
|
| termination of elongation |
can be mediated either by |
an acyl-ACP thioesterase (FAT)—resulting in free fatty acid release and export to the cytosol—or by direct transfer of the acyl group to glycerol-3-phosphate and/or monacylglycerol-3-phosphate in the TAG biosynthetic pathway |
Haematococcus pluvialis |
| fatty acid elongase 1 (FAE1, KCS18, AT4G34520) |
catalyses the production of |
C20+ very-long-chain saturated and mono-unsaturated fatty acids (VLCFAs) |
Arabidopsis thaliana |
| pdh lines |
produced 45–75% less volumetric total fatty acid than |
control lines in HSM-N |
Chlamydomonas reinhardtii |
| pdh lines |
had total fatty acid production unaltered in |
mixotrophic nitrogen-depleted cultures compared with control |
Chlamydomonas reinhardtii |
| cpPDC |
is at least one control point in |
supply of acetyl-CoA for de novo FA synthesis |
|
| heterotrophic growth in the absence of chloroplast translation |
is enhanced by |
genetic modifiers present in the most tolerant accessions |
Arabidopsis thaliana |
| DG2 and PD1a |
did not achieve FA synthesis levels similar to |
(FAE1, KCS18, AT4G34520) |
|
| biotin |
is required for |
ACCase function |
Arabidopsis thaliana |
| (BADC1, BLP3, AT3G56130) (BADC3, BLP2, AT3G15690) double mutant seeds |
show |
fatty acid increases of 18% to 30% |
Arabidopsis thaliana |
| heterologous expression of S. foetida (ABC33, ATCPS1, CPS, CPS1, GA1, AT4G02780) |
resulted in accumulation of detectable |
cyclopropane fatty acid (CPA) |
Arabidopsis thaliana |
| T411 mutant line |
diverts carbon via enhanced glycolysis for |
FFA synthesis |
Chlamydomonas reinhardtii |
| Rhizophagus irregularis |
lacks |
gene encoding multidomain fatty acid de novo synthase |
Rhizophagus irregularis |
| PDC2_E1α silencing |
resulted in |
about 20% lower total fatty acid content in pdh72 after 6 days photoautotrophic growth |
Chlamydomonas reinhardtii |
| pdh mutants |
demonstrated |
25–40% less volumetric total fatty acid production than control lines |
Chlamydomonas reinhardtii |
| FAs |
are synthesized in |
plastids |
|
| (ATMYB118, MYB118, PGA37, AT3G27785) |
is essential for |
biosynthesis of omega-7 monounsaturated fatty acids |
Arabidopsis thaliana |
| Arabidopsis thaliana mutants lacking WRINKLED1 (ASML1, ATWRI1, WRI, WRI1, AT3G54320) |
show restoration of oil content with |
respective orthologs from maize |
Arabidopsis thaliana; Zea mays |
| fad3-2 mutant |
is impaired in |
α-linolenic acid biosynthesis |
Arabidopsis thaliana |
| fad2fae1 Arabidopsis seeds |
contain 18:1 at approximately |
84% of total fatty acid |
Arabidopsis thaliana |
| 3-ketoacyl-ACP synthase gene |
has |
approximately two copies in plastids |
Brassica napus |
| IgASE1 expression |
leads to production of |
EDA and ETrA |
Arabidopsis thaliana |
| pdh lines |
demonstrated severe impairment of |
total fatty acid production in HSM-N |
Chlamydomonas reinhardtii |
| reduction in SUC5-driven sucrose import |
results in |
reduced availability of organic carbon for fatty acid biosynthesis |
Arabidopsis thaliana |
| tissue-specific enrichment in capacity for 18:1 formation and export from plastids |
appears to be higher in |
cotyledons at transcriptional level |
Brassica napus |
| same unusual fatty acid in seed oils |
can be achieved by |
action of evolutionary unrelated enzymes |
|
| suppressed CsFAD3 expression |
resulted in |
lower levels of 18:3 accumulation |
Camelina sativa |
| acyl-ACP chain |
is successively elongated by a subsequent series of condensation reactions catalysed by |
3-ketoacyl-ACP-synthase (KAS), 3-ketoacyl-ACP-reductase (KAR), 3-hydroxyacyl-ACP dehydratase (HD), and enoyl-AC reductase (ENR) |
Haematococcus pluvialis |
| Acer truncatum KCS gene family |
expanded to |
28 genes |
Acer truncatum |
| successful engineering of tomato trichomal methylketone production |
might require introducing |
wild tomato fatty acid biosynthesis gene loci |
Solanum lycopersicum; Solanum habrochaites |
| up-regulation of most genes involved in the FA biosynthetic pathway |
suggests that the direction of carbon flux is toward |
FFA biosynthesis |
Chlamydomonas reinhardtii |
| ferredoxin (Fd) |
is involved in |
fatty acid biosynthesis |
|
| fatty acid biosynthesis |
occurs in |
chloroplast |
|
| oleoyl chains |
are produced within |
chloroplast |
|
| unusual fatty acids with epoxy and hydroxy groups, triple bonds and conjugated double bonds |
are synthesized from |
enzymes that have evolved from an ancestral Δ12 fatty acid desaturase |
|
| lower levels of 18:3 accumulation |
in |
miR167OE seeds |
Camelina sativa |
| seed-specific expression of RNAi constructs |
targeting the three B. napus FAD2 genes allow for |
maintenance of wild-type levels of polyunsaturated fatty acids in non-seed tissues |
Brassica napus |
| acetyl-CoA, NADH, and NADPH |
power |
two-carbon elongation of fatty acid chain in plastid |
Glycine max |
| pyruvate |
is the precursor of |
acetyl-CoA |
Glycine max |
| ACPs as a class |
increase during |
fatty acid biosynthesis |
Glycine max |
| AtME4 events |
results in enhanced levels of |
oleic acid |
Glycine max |
| (ACC2, AT1G36180) protein |
participates in |
fatty acid biosynthesis |
Arabidopsis thaliana |
| cerulenin |
forms covalent bond with |
catalytic cysteine of fatty acid synthase |
|
| very-long-chain fatty acid (VLCFA)-derivatives |
are obtained from |
plastidial fatty acid de novo biosynthesis |
|
| 68 genes important for VLCFA biosynthesis pathway |
were identified in |
Acer truncatum |
Acer truncatum |
| Camelina sativa derived fatty acid desaturase 3 overexpression (CsFAD3-OE) Arabidopsis line |
accumulated high levels of |
α-linolenic acid (ALA) |
Arabidopsis thaliana |
| NADPH-producing malic enzyme (ME) and pyruvate dehydrogenase complex (PDH) |
deliver stoichiometric amounts of |
NADPH |
Glycine max |
| labeling in pyruvate but not in (PAS2, PEP, PEPINO, AT5G10480) from LC–MS/MS analysis of U-13C glutamine cultured soybeans |
supported |
malic enzyme, not (PCK2, PEPCK, AT5G65690) supported fatty acid biosynthesis |
Glycine max |
| acyl-ACPs |
are located in |
chloroplast |
|
| C14 (ACP, ACP1, AtACP1, AT3G05020) accumulation |
possibly results from |
increased nonspecific (AtFaTA, FaTA, FATA1, AT3G25110) /B thioesterase activity |
Glycine max |
| (ACP, ACP1, AtACP1, AT3G05020) profile observed in soybeans |
differs from |
other reports |
Glycine max |
| measured increase in lipid in soybeans |
does not require changes to |
fatty acid biosynthetic machinery |
Glycine max |
| KASII |
drives |
conversion of 16:0-ACP to 18:0-ACP |
Camelina sativa |
| many fungi (e.g. Saccharomyces cerevisiae or Aspergillus nidulans) |
have enzymatic domains of FAS distributed to |
two polypeptides (α and β subunits, type A FAS) |
Saccharomyces cerevisiae; Aspergillus nidulans |
| genetic engineering |
enables |
generation of unique oils in commodity crops |
Glycine max |
| altered expression of genes involved in flavonoid and fatty acid biosynthesis |
results in notable increases of |
linoleic acid (C18:2) |
Brassica napus |
| plastidial pyruvate dehydrogenase (PDH) |
catalyzes decarboxylation of |
pyruvate |
|
| de novo fatty acid biosynthesis |
is localized in |
chloroplasts |
|
| MYB and (AtbZIP, bZIP, AT1G68880) transcription factors (TFs) |
participate in regulating |
synthesis of fatty acids |
|
| enhancement of expression/activity of KASII |
could increase |
18:1-ACP/18:1-CoA pools for further desaturation |
Camelina sativa |
| di-hydroxy fatty acids |
has |
described biosynthetic pathway |
|
| levels of enzymes involved in fatty acid synthesis |
are unchanged in |
(ICL, AT3G21720) mutant |
Chlamydomonas |
| transient decrease in fatty acid content in (ATSUC5, SUC5, AT1G71890) mutants at 8 DAF |
coincides with |
onset of fatty acid biosynthesis |
Arabidopsis thaliana |
| mammals |
contain |
single FAS polypeptide of about 2500 amino acids (type I FAS) |
|
| type II mitochondrial FAS |
consists of |
individual polypeptides including separate acyl carrier protein (ACP, ACP1, AtACP1, AT3G05020) |
|
| (AtFAD3, FAD3, AT2G29980) expression levels |
corresponded to |
18:3 accumulation |
Arabidopsis thaliana |
| (ATMYB118, MYB118, PGA37, AT3G27785) |
regulates transcription of |
(AAD2, AT3G02610) and (AAD3, AT5G16230) |
Arabidopsis thaliana |
| soybean events carrying only the Delta(15) desaturase |
possessed |
significant elevation of alpha-linolenic acid (ALA) content |
Glycine max |
| some fungi (Laccaria bicolor, Ustilago maydis) |
contain |
single polypeptide FAS analogous to animals (type B1 FAS) |
Laccaria bicolor; Ustilago maydis |
| Brassicaceae |
accumulate |
very long chain fatty acids |
|
| (AP2, AtAP2, FL1, FLO2, AT4G36920) /EREB targets |
are involved in biosynthesis of |
FAS |
Zea mays |
| cpPDC |
is at least one control point in |
supply of acetyl-CoA for de novo FA synthesis in the plastids of C. reinhardtii |
Chlamydomonas reinhardtii |
| lack of biotin in the embryo |
may cause |
transient decrease in fatty acid content in (ATSUC5, SUC5, AT1G71890) mutants at 8 DAF |
Arabidopsis thaliana |
| imported sugars and amino acids |
are converted to |
fatty acids |
Glycine max |
| pyruvate dehydrogenase |
is linked specifically to |
fatty acyl chain biosynthesis |
Glycine max |
| (CTI1, AT1G42960) interaction with ACCase |
mediates |
docking of ACCase to the plastid envelope membrane |
Arabidopsis thaliana |
| α-ESA under canopy light |
remained at |
<50% until very near the end of seed development |
|
| conversion of acetyl-CoA to malonyl-CoA during fatty acid biosynthesis within the plastid |
is required for |
embryo development in Arabidopsis |
Arabidopsis thaliana |
| heterotrophic growth in the absence of chloroplast translation |
is mediated by |
(ACC2, AT1G36180) |
Arabidopsis thaliana |
| function of ShMKS2 |
is |
thioesterase utilizing fatty acid biosynthesis intermediates |
|
| homozygous T3 seeds expressing EcCPS |
accumulated approximately twice as much |
cyclopropane fatty acid (CPA) compared to hemizygous T1 seeds |
Arabidopsis thaliana |
| enrichment of cyclopropane fatty acid (CPA) at both sn-1 and sn-2 positions of phosphatidylcholine (PC) |
increases |
accumulation of cyclopropane fatty acid (CPA) |
|
| malvic acid in S. foetida |
is present at only |
3% at sn-2 position |
Sterculia foetida |
| (BADC1, BLP3, AT3G56130) knockout |
results in significant increase in |
seed fatty acid content as percentage dry weight |
Arabidopsis thaliana |
| α-ESA |
is also produced in |
developing seed cotyledons |
|
| phosphatidylcholine |
is the site of |
hydroxy fatty acid biosynthesis |
|
| GmSACPD-A, GmSACPD-B, and GmSACPD-D mutants |
have |
lower stearic acid content (4–7%) than Gmsacpd-c mutants |
Glycine max |
| ShMKS2 |
is mostly |
β-ketoacyl-acyl-carrier protein thioesterase preferring C14 substrate |
|
| flufenacet |
inhibits |
activity of very-long-chain fatty acid elongases |
|
| (ACC2, AT1G36180) |
targets |
homomeric acetyl-coenzyme A carboxylase (ACCase) |
Arabidopsis thaliana |
| (ACC2, AT1G36180) contributions to fatty acid biosynthesis |
impact on |
plant growth and development in natural environments |
Arabidopsis thaliana |
| AMPK |
inhibits |
fatty acid synthase |
Chlamydomonas reinhardtii |
| (BADC1, BLP3, AT3G56130) (BADC3, BLP2, AT3G15690) double mutant |
shows significant increase in |
fatty acid per seed |
Arabidopsis thaliana |
| enhancer and modifiers |
could lead to |
improved targeting of overexpressed homomeric acetyl-coenzyme A carboxylase to plastids in transgenic plants |
|
| model oilseeds |
are used for |
biosynthesis of modified fatty acids |
Arabidopsis thaliana |
| (BADC1, BLP3, AT3G56130) (BADC2, BLP1, AT1G52670) double mutant |
shows significantly elevated |
fatty acid dry weight |
Arabidopsis thaliana |
| fatty acids |
are synthesized in |
chloroplast |
|
| TAG accumulation through FA biosynthesis |
is |
highly energy-consuming process |
Chlamydomonas reinhardtii |
| crown galls |
produce increased levels of |
α-linolenic acid |
Arabidopsis thaliana |
| up-regulation of FA biosynthesis genes in T411–N |
substantiates |
higher FFA levels |
Chlamydomonas reinhardtii |
| (ATVPS34, PI3K, VPS34, AT1G60490) KD |
resulted in AMPK down-regulation and enhanced |
FA biosynthesis |
Chlamydomonas reinhardtii |
| RcFAH12 expression in (FAE1, KCS18, AT4G34520) mutant |
produces seeds with |
elevated 18:1 |
Arabidopsis thaliana |
| KAR (3-ketoacyl-ACP reductase) |
is part of |
plastid fatty acid synthase complex |
Physcomitrella patens |
| acetyl-CoA |
cannot cross |
plastid membrane |
|
| fatty acid (FA) biosynthesis |
is enriched in |
CsFAD3-OE embryos |
Camelina sativa |
| FD |
is required for |
fatty acid biosynthesis |
|
| Tree peony (Paeonia suffruticosa Andr.) |
has |
high proportion of ALA (more than 45% in seed oil) |
Paeonia suffruticosa |
| medium-chain and very-long-chain fatty acids |
are synthesized by |
variants of enzymes (thioesterases and fatty acid elongases) expressed in all plants |
|
| Crambe seed oil |
is naturally enriched in |
erucic acid (22:1; ∼60% of total oil) |
Crambe abyssinica |
| depletion of dioxygen |
limits |
de novo synthesis of unsaturated fatty acids (FAs) |
|
| acetyl-CoA generated from acetate in the medium |
was rerouted to |
FA biosynthesis under acute starvation conditions |
Chlamydomoans reinhardtii |
| pyruvate dehydrogenase (PDH) |
provides acetyl-CoA for |
fatty acid biosynthesis |
|
| disruption of (115D-4A, ACC1, AT-ACC1, EMB22, GK, GSD1, PAS3, SFR3, AT1G36160) gene in Arabidopsis ( GURKE, and PASTICCINO3 [ ]) |
results in |
embryo-defective phenotype distinct from that seen following a loss of chloroplast translation |
Arabidopsis thaliana |
| increases in methylketone production in plants |
may also require increasing |
flux of fatty acid biosynthesis |
|
| maximal levels of methylketones |
depends on presence of wild tomato alleles at |
fatty acid biosynthesis gene loci |
Solanum lycopersicum; Solanum habrochaites |
| morphological and developmental phenotypes of ShMKS2-expressing plants |
are quite similar to |
phenotypes of Arabidopsis mutants deficient in de novo fatty acid biosynthesis |
Arabidopsis thaliana |
| genetic modification of stearic acid biosynthesis pathway |
is more efficient in |
reducing trans-fats introduced by hydrogenation process |
Glycine max |
| homomeric acetyl-coenzyme A carboxylase (ACCase) |
localizes to |
plastids |
Arabidopsis thaliana |
| β-ketoacyl-acyl-carrier protein synthase1 mutant |
displays |
growth retardation, variegated leaves, and reduced fertility |
Arabidopsis thaliana |
| up-regulation of the gene encoding Pyruvate dehydrogenase, (PDC2, AT5G54960) |
confirms the direction of carbon flux toward |
FA biosynthesis |
Chlamydomonas reinhardtii |
| three additional genes ( (LTA2, PLE2, AT3G25860) (EMB3003, AT1G34430) and (EMB3147, MCAMT, MCAT, AT2G30200) ) |
are associated with |
reactions that precede and follow the step catalyzed by heteromeric ACCase |
Arabidopsis thaliana |
| interference with fatty acid biosynthesis |
remains a possible explanation for |
lesions and delayed growth |
Arabidopsis thaliana; Nicotiana tabacum; Solanum lycopersicum |
| low temperature (15°C) |
facilitates |
accumulation of 20:5 |
Nannochloropsis oceanica |
| (115D-4A, ACC1, AT-ACC1, EMB22, GK, GSD1, PAS3, SFR3, AT1G36160) |
encodes |
essential protein localized to the cytosol |
Arabidopsis thaliana |
| (BADC2, BLP1, AT1G52670) mutant |
shows elevation in |
levels of fatty acid dry weight in seeds |
Arabidopsis thaliana |
| functional OPPP and dark regulation of (PRK, AT1G32060) in Vaucheria litorea plastids |
is essential for |
synthesis of NADPH for fatty acid biosynthesis |
Vaucheria litorea |
| heterologous expression of cotton (ABC33, ATCPS1, CPS, CPS1, GA1, AT4G02780) |
resulted in accumulation of detectable |
cyclopropane fatty acid (CPA) |
Arabidopsis thaliana |
| transcripts of several fatty acid biosynthesis genes |
are present at lower concentrations in |
trichomes of cultivated tomato |
Solanum lycopersicum; Solanum habrochaites |
| localization pattern of KAR in stromules |
indicates |
demand of fatty acid synthesis |
Physcomitrella patens |
| growth defects of transgenic Arabidopsis overexpressing ShMKS2 |
are likely a result of |
interruption of fatty acid biosynthesis |
Arabidopsis thaliana |
| current study |
showed potential effect of |
new isolated Gmsacpd mutants in increasing seed stearic acid content while maintaining healthy nodules |
Glycine max |
| fatty acid desaturase (AtFAD3, FAD3, AT2G29980) |
is primarily responsible for |
production of 18:3 |
|
| bzip67 mutant seed |
contained |
lower 18:3 |
Arabidopsis thaliana |
| bryophytes |
have propensity for |
very long chain polyunsaturated fatty acid (vlcPFA) accumulation |
|
| disruptions of three additional genes ( (LTA2, PLE2, AT3G25860) (EMB3003, AT1G34430) and (EMB3147, MCAMT, MCAT, AT2G30200) ) |
result in |
embryo lethality |
Arabidopsis thaliana |
| constitutive expression of ShMKS2, either with or without ShMKS1, in transgenic Arabidopsis |
led to |
myristic acid accumulation in planta |
Arabidopsis thaliana |
| (ATVPS34, PI3K, VPS34, AT1G60490) KD |
showed |
enhanced/uninhibited FFA synthesis |
Chlamydomonas reinhardtii |
| unusual fatty acids |
are synthesized by |
enzymes that have evolved as variants of enzymes of membrane fatty acid metabolism |
|
| Crepis palaestina |
synthesizes vernolic acid from |
linoleic acid via a divergent (AtFAD2, FAD2, AT3G12120) desaturase-like enzyme |
Crepis palaestina |
| FAS1-like complexes |
have been suggested in |
oleaginous species Nannochloropsis oceanica and Nannochloropsis gaditana |
Nannochloropsis oceanica; Nannochloropsis gaditana |
| R5 seeds |
showed higher levels of |
short-chain ACPs |
Glycine max |
| mutations in the soy FAD2-1A and FAD2-1B genes |
has led to the production of |
high-oleic lines |
Glycine max |
| (ACC2, AT1G36180) |
may contribute to |
fatty acid biosynthesis in chloroplasts under selected conditions |
Arabidopsis thaliana |
| acetyl-CoA |
was partly directed toward |
FA synthesis |
Chlamydomonas reinhardtii |
| acyl carrier protein 1 (ACP, ACP1, AtACP1, AT3G05020) gene |
has |
16 copies in plastids |
Brassica napus |
| transcript ratio similarity between 9 DAF and 20-32 DAF seeds |
indicates |
active fatty acid metabolism at 9 DAF |
Brassica napus |
| C18 fatty acids |
are produced through |
acetate pathway |
|
| fatty acid biosynthesis |
requires |
balanced supply of carbon and reducing equivalents |
|
| malic enzyme |
is linked specifically to |
fatty acyl chain biosynthesis |
Glycine max |
| (FATB, AT1G08510) transcripts |
were higher in |
EA tissues |
Brassica napus |
| metabolic rearrangement under HL versus LL conditions |
leads to |
enhanced de novo fatty acid biosynthesis |
Chlorella vulgaris |
| cytosolic fatty acid biosynthetic pathway |
is |
predicted pathway |
Chlorella vulgaris |
| deletion in GmSACPD-B |
resulted in |
high stearic acid content in seeds |
Glycine max |
| very long chain fatty acids (>C20) |
has |
described biosynthetic pathway |
|
| decreased 18:3 content |
corresponded to |
reduced CsFAD3 transcripts |
Camelina sativa |
| FAS1-like complexes |
have not yet been suggested in |
green lineage |
|
| nonsense mutations in GmSACPD-C |
increasing |
seed stearic acid content up to 20.7% |
Glycine max |
| epoxy fatty acids |
has |
described biosynthetic pathway |
|
| Crepis palaestina |
accumulates |
about 60% vernolic acid in its seed oil |
Crepis palaestina |
| relative enrichment of C18:1 acyl groups in cotyledonary tissues |
may in part be contributed from |
final steps in de novo FA biosynthesis in plastid |
Brassica napus |
| acetate pathway |
gets its substrate from |
acetyl-CoA |
|
| oleate desaturase (AtFAD2, FAD2, AT3G12120) gene |
modifications in |
biosynthesis of hydroxy fatty acids |
|
| animals and fungi |
possess |
FAS type I complexes (FAS1) located in cytosol |
|
| acetyl-coenzyme A carboxylase (ACCase) |
is |
essential enzyme in fatty acid biosynthesis |
Arabidopsis thaliana |
| expression of E. coli cyclopropane synthase (CPS) gene in fad2fae1 Arabidopsis |
results in accumulation of as much as |
9.3% cyclopropane fatty acid (CPA) in Arabidopsis seeds |
Arabidopsis thaliana |
| AMPK |
inhibits |
acetyl-CoA carboxylase |
Chlamydomonas reinhardtii |
| (KAS1, KASI, AT5G46290) and (ATKAS2, FAB1, KAS2, AT1G74960) |
show unchanged levels in |
(ICL, AT3G21720) mutant |
Chlamydomonas |
| Q215W mutant (AtFaTA, FaTA, FATA1, AT3G25110) |
has higher catalytic efficiency compared to |
wild-type Helianthus annuus (AtFaTA, FaTA, FATA1, AT3G25110) |
Helianthus annuus |
| active starch metabolism at early stages of Brassica napus seed development |
is consistent with |
differential expression patterns of starch metabolism and OPPP-related genes |
Brassica napus |
| fatty acid biosynthesis |
is |
one of the major steps involved in production of complex oils |
|
| combined results of genomic, transcriptomic, cell ultrastructural, and gas chromatography–mass spectrometry analyses |
provided |
new insights regarding fatty acid biosynthesis pathway in Acer truncatum |
Acer truncatum |
| enzyme-encoding gene families related to lipid metabolism |
underwent more than three duplications |
(ATTSC13, CER10, ECR, GLH6, TSC13, AT3G55360) ER, HAD, KAR, SAD, LACS, and KCS |
Acer truncatum |
| acetyl-coenzyme A carboxylase D (ACCD, ATCG00500) |
underlies the requirement for |
chloroplast translation during heterotrophic growth and embryo development |
Arabidopsis thaliana |
| (ACC2, AT1G36180) |
might lack |
feedback regulation system described for heteromeric acetyl-coenzyme A carboxylase in the Brassicaceae |
Arabidopsis thaliana |
| fatty acid synthesis |
usually terminates with |
C16:0 or C18:0 products |
|
| fatty acid desaturase (FAD) |
is an Fd-dependent gene |
Fd-dependent metabolic pathway |
Oryza sativa |
| Gene Ontology (GO) term analysis of DEGs for upper versus lower epidermal cells |
revealed |
biological processes associated with photosynthesis, cuticle development, and fatty acid biosynthesis |
Arabidopsis thaliana |
| fatty acid synthase (FAS) |
functions in |
de-novo biosynthesis of fatty acids |
|
| ShMKS2 |
is capable of hydrolyzing |
myristoyl-acyl-carrier protein |
|
| active starch metabolism at early stages of Brassica napus seed development |
occurs before |
initiation of fatty acid biosynthesis |
Brassica napus |
| redox states of the chloroplast and (ATPRX Q, PRXQ, AT3G26060) |
connecting to |
16:1t synthesis |
|
| plastidial acetate transporter |
has not been identified |
to date |
|
| de novo fatty acid biosynthesis |
requires |
reducing power |
|
| (GLC, AT1G65450) 6P |
is able to support |
fatty acid biosynthesis |
|
| (PAS2, PEP, PEPINO, AT5G10480) |
is able to support |
fatty acid biosynthesis |
|
| KCS gene |
is important for |
nervonic acid biosynthesis |
|
| nuclear gene |
encodes |
chloroplast-localized homomeric acetyl-coenzyme A carboxylase (ACCase) |
Poaceae |
| enoyl-acyl-carrier protein reductase mutant mosaic death1 |
displays |
growth retardation, variegated leaves, and reduced fertility |
Arabidopsis thaliana |
| lower oil content in HFA-accumulating lines |
provides one explanation for |
reduced FA synthesis |
|
| T-DNA insertions into (BADC1, BLP3, AT3G56130) (BADC2, BLP1, AT1G52670) and (BADC3, BLP2, AT3G15690) genes |
resulted in |
increased fatty acid accumulation in seeds |
Arabidopsis thaliana |
| α-ESA (alpha-eleostearic acid) |
accumulated to approximately 80% of oil component under rich light after 1 week of rapid oil accumulation |
tung seed oil |
|
| ACCase docking to the plastid envelope membrane |
attenuates |
fatty acid biosynthesis |
Arabidopsis thaliana |
| defective embryo |
shows inhibition of |
fatty acid synthesis |
Arabidopsis thaliana |
| MYB and (AtbZIP, bZIP, AT1G68880) transcription factors (TFs) |
were found to be involved in regulating |
nervonic acid biosynthesis |
Acer truncatum |
| beta-ketoacyl-ACP reductase |
catalyzes reduction of |
beta-ketoacyl-CoA to beta-hydroxyacyl-CoA |
|
| CsFAD3 |
might be subject to regulation by |
miR167A-CsARF8 mediated pathways |
Camelina sativa |
| enoyl-ACP reductase (ENR) gene |
has |
approximately two copies in plastids |
Brassica napus |
| histone acetylation |
might contribute to |
optimization of nutritional structure of edible oils through epigenetic engineering |
Arabidopsis thaliana |
| (HDA2, AT5G26040) mutant |
shows variation in |
total fatty acid content |
Arabidopsis thaliana |
| β-ketoacyl-ACP synthase (KAS) |
is involved in |
elongation of carbon chain from C4 to C18 |
|
| 3-oxyacyl- (ACP, ACP1, AtACP1, AT3G05020) reductase (KR), enoyl- reductase (ENR), and acyl carrier protein 1 genes |
have transcript ratio of |
KR:ENR:ACP = 1:1:8 |
Brassica napus |
| plastids |
are involved in |
fatty acid biosynthesis |
|
| 3-oxyacyl- (ACP, ACP1, AtACP1, AT3G05020) reductase (KR), enoyl- reductase (ENR), and acyl carrier protein 1 genes |
is similar to |
transcript ratio of KR:ENR:ACP = 1:1.2:6.9 in seeds at 20-32 DAF |
Brassica napus |
| de novo fatty acid biosynthesis |
requires |
ATP |
|
| ATP and reducing equivalents |
are generated by |
substrate-level phosphorylation |
|
| cultured Arabidopsis embryos |
show decrease in |
long chain fatty acid accumulation |
|
| (ATX4, SDG16, AT4G27910) mutant |
shows variation in |
total fatty acid content |
Arabidopsis thaliana |
| constitutive expression of (BGT, GCN5, HAC3, HAG01, HAG1, HAT1, AT3G54610) in wild-type plants |
increased |
ALA/LA ratio |
Arabidopsis thaliana |
| ONI1 and ONI2 |
encode |
homologs of FA condensing enzymes |
Oryza sativa |
| genes encoding the FA condensing enzymes |
include |
large number of homologs (around 20 or more homologous sequences have been identified in the genome of rice and other plants) |
Oryza sativa |
| (AtFAP1, FAP1, AT3G63170) |
is |
FA-binding protein which localizes in plastid stroma |
|
| GRMZM5G853065 |
encodes |
fatty acid synthase (FAS, EC: 2.3.1.85) |
Zea mays |
| de novo fatty acid biosynthesis |
is localized in |
non-green plastids |
|
| (FAD4, FADA, AT4G27030) mutant |
was first discovered through |
screen for changes in total leaf FA composition |
Arabidopsis thaliana |
| 3-oxyacyl-ACP reductase (KR) gene |
has |
approximately two copies in plastids |
Brassica napus |
| lipid biosynthesis capacity in tomato chromoplast |
is attested by presence of |
all acetyl-CoA-carboxylase monomers |
Solanum lycopersicum |
| coexpression of EcCPS with SfLPAT |
suggests that substrate supply is not limiting for |
cyclopropane fatty acid (CPA) accumulation |
|
| (BADC2, BLP1, AT1G52670) mutant |
shows smaller increase in |
seed fatty acid content relative to wild type |
Arabidopsis thaliana |
| high-throughput sequencing of differentially expressed genes |
revealed |
altered expression of genes involved in fatty acid biosynthesis |
Brassica napus |
| expansion of resources through increased 0-HFA-TAG and common FA availability |
leads to |
greater FA synthesis and decreased retention of HFA in the polar lipid during establishment |
|
| desaturation and elongation of long-chain fatty acids |
proceed at |
endoplasmic reticulum (ER) membrane |
Nannochloropsis oceanica |
| cytosolic, homomeric ACCase |
is involved in |
later stages of fatty acid biosynthesis |
Arabidopsis thaliana; Brassica napus |
| acetyl-CoA synthase proteins |
are involved in |
early stages of de novo fatty acid biosynthesis |
Dunaliella bardawil |
| (BADC3, BLP2, AT3G15690) mutant |
shows significant increase in |
fatty acid per seed |
Arabidopsis thaliana |
| production of myristic acid |
was observed in |
all three plant species expressing ShMKS2 under the 35S promoter |
Arabidopsis thaliana; Nicotiana tabacum; Solanum lycopersicum |
| highly expressed seed isoform GmSACPD-C mutants at M6 generation |
were not able to recover and/or restore phenotype and showed |
greater than 21% stearic acid content |
Glycine max |
| mutations of histone methylation- or acetylation-related genes |
resulted in |
highly variable fatty acid (FA) contents and compositions |
Arabidopsis thaliana |
| NF-YB-1 |
is highly expressed in |
mesocarp and endosperm at stages when FAS gene transcription peaked |
Elaeis guineensis |
| KASII |
is the only gene that clearly opposed |
16:0 |
Elaeis guineensis |
| low rates of FA synthesis |
results in |
high concentrations of (PAS2, PEP, PEPINO, AT5G10480) and 3-PGA |
|
| GRMZM2G120987 |
encodes |
fatty acyl carrier protein reductase (FAR2, MS2, AT3G11980) |
Zea mays |
| fatty acid synthase (FAS) |
catalyzes |
formation of 16:0 and 18:0 fatty acids |
|
| soft impact on GmSACPD-C protein activity |
subsequently resulting in |
slight increase in seed stearic acid (~7%) |
Glycine max |
| (BGT, GCN5, HAC3, HAG01, HAG1, HAT1, AT3G54610) mutation |
decreases |
ratio of α-linolenic acid (ALA) to linoleic acid (LA) |
Arabidopsis thaliana |
| (AtFAD3, FAD3, AT2G29980) (LACS2, LRD2, AT1G49430) (ATLPP3, LPP3, AT3G02600) and PLAIII β |
are |
targets of (BGT, GCN5, HAC3, HAG01, HAG1, HAT1, AT3G54610) involved in fatty acid synthesis |
Arabidopsis thaliana |
| (BCCP2, CAC1-B, AT5G15530) |
is downregulated in |
wri4-1 mutant stems |
Arabidopsis thaliana |
| ZFP-1 |
is linked to |
10 guide genes |
Elaeis guineensis |
| (AtFAP3, FAP3, AT1G53520) |
was coexpressed with |
FAS genes in Arabidopsis |
Arabidopsis thaliana |
| (ATSUC5, SUC5, AT1G71890) mutants ( .1, .2 and .3) |
show transient decrease in |
total fatty acid content at 8 DAF |
Arabidopsis thaliana |
| (AtPPT1, HRL1, PPT1, AT4G23660) overexpression in wild-type Col-0 |
did not increase |
fatty acid content |
Arabidopsis thaliana |
| mutation in (ATX5, SDG29, SET29, AT5G53430) |
significantly increased |
total fatty acid (FA) content |
Arabidopsis thaliana |
| histone methylation |
might be involved in |
fatty acid (FA) biosynthesis |
Arabidopsis thaliana |
| (WRI4, AT1G79700) |
upregulates |
genes encoding enzymes associated with fatty acid biosynthesis |
Arabidopsis thaliana |
| substrate preferences of oil palm (AtFaTA, FaTA, FATA1, AT3G25110) and FATB1 and B2 isoforms |
remain unknown |
|
Elaeis guineensis |
| EgWRI1-1 |
is |
transcriptional enhancer of fatty acid biosynthesis genes |
Elaeis guineensis; Arabidopsis thaliana |
| transcripts for KASII |
were higher in |
cotyledons relative to EA tissues |
Brassica napus |
| fatty acid biosynthetic genes |
show differential expression between |
M+ and M− plants |
Medicago truncatula |
| stearoyl-ACP-desaturase gene |
has |
approximately two copies in plastids |
Brassica napus |
| genes involved in lipid and sugar metabolism |
are most linked to |
at least two of the three transcription factors |
Elaeis guineensis |
| LC-PUFAs |
engineered to economically viable levels in |
camelina seeds |
Camelina sativa |
| Arabidopsis (AtFAD2, FAD2, AT3G12120) (FAE1, KCS18, AT4G34520) mutant |
has |
high levels of oleic acid in seeds |
Arabidopsis thaliana |
| constitutive expression of ShMKS2, either with or without ShMKS1, in transgenic tobacco |
led to |
myristic acid accumulation in planta |
Nicotiana tabacum |
| constitutive down-regulation of ACCase by BADC |
supported by |
observation that rates of fatty acid synthesis in (BADC1, BLP3, AT3G56130) (BADC3, BLP2, AT3G15690) are 26% higher than wild-type |
Arabidopsis thaliana |
| Δ12 desaturases (AtFAD2, FAD2, AT3G12120) |
catalyzes production of |
unusual fatty acids |
|
| rate of fatty acid synthesis with malate as precursor |
approximately 4.5 and 120 times higher than |
rate of fatty acid synthesis with pyruvate and acetate as precursors |
|
| PbFAR3 (accession number 103931004) |
transcript levels are significantly higher in |
rainfall-treated russet and semi-russet fruit than in control fruit |
Pyrus × bretschneideri |
| altered fatty acid (FA) biosynthesis |
is involved in |
rainfall-induced russeting in sand pear fruit |
Pyrus × bretschneideri |
| VfHB21 overexpression |
causes downregulation of |
(AtLSM5, AtSAD1, LSM5, SAD1, AT5G48870) |
Arabidopsis thaliana |
| non-productive, competing pathways |
possibly down-regulated in seeds of host oilseeds |
seeds of host oilseeds |
|
| fatty acid structures in plant seed oils |
has guided discovery of |
novel biosynthetic enzymes |
|
| DEGs involved in fatty acid biosynthesis |
were expressed at very low levels prior to oil onset and then sharply upregulated in early days of rapid oil accumulation period |
oil accumulation onset |
|
| fatty acid metabolic pathways |
requires in-depth understanding in seeds that naturally accumulate high levels of unusual fatty acids |
solving bottlenecks that limit synthesis and accumulation of unusual fatty acids |
|
| cryoelectron microscopy |
is emerging technique that can be applied to |
fatty acid elongase complex and putative acyltransferase metabolons |
|
| NMR and protein–protein docking simulations |
used to characterize |
interactions present during E. coli fatty acid biosynthesis |
Escherichia coli |
| light-upregulated DEGs |
showed enrichment of |
carbon metabolism, biosynthesis of amino acid, fatty acid metabolism, and fatty acid biosynthesis pathway genes |
|
| three genes identified by Xu et al. (2019) |
were duplicated and predicted to be important in regulating |
VLCFA biosynthesis pathway in Moringa oleifera |
Moringa oleifera |
| fatty acid (FA) synthesis pathway |
is inhibited with |
up to 30 downregulated differentially expressed genes (DEGs) |
Arabidopsis thaliana |
| removal of (CTI1, AT1G42960) |
leads to enhanced |
de novo fatty acid biosynthesis |
Arabidopsis thaliana |
| transcription factors that up regulate fatty acid synthesis |
engineering of |
fatty acid synthesis |
|
| novel biosynthetic enzymes |
have provided insights into |
how structural variations can lead to alternative catalytic outcomes |
|
| α-ESA in developing seed cotyledons |
makes up approximately 50% of |
fatty acid components |
|
| reduced (CTI1, AT1G42960) level in the envelope membrane |
should result in enhanced |
de novo fatty acid biosynthetic flux |
Arabidopsis thaliana |
| enhanced de novo fatty acid biosynthesis rate |
is likely caused by |
(CTI1, AT1G42960) and cti123 mutants |
Arabidopsis thaliana |
| dense shade |
causes repression of |
genes involved in de novo FA biosynthesis |
Vernicia fordii |
| oleic acid |
remained largely equal between |
rich light and canopy light samples |
|
| engineered seeds of crop and model plant species |
produce |
long-chain omega-3 polyunsaturated fatty acids |
|
| FADX |
catalyzes |
conjugation of linoleic acid to produce α-ESA |
|
| linoleic acid produced by (AtFAD2, FAD2, AT3G12120) |
serves as substrate for |
FADX |
|
| very long-chain fatty acid (VLCFA) production |
bypasses |
PC-linked biosynthetic pathways |
|
| soybeans carrying mutations in GmSACPD-A, GmSACPD-B, and GmSACPD-D genes |
are potential new sources of |
high seed stearic acid while retaining good seed yield |
Glycine max |
| Acer truncatum |
produces seeds with |
high levels of valuable fatty acids |
Acer truncatum |
| fatty acid biosynthesis-related genes |
were systematically identified in |
Brassica napus |
Brassica napus |
| phosphatidylcholine |
is site of synthesis of |
novel fatty acids |
|
| (NTRC, AT2G41680) /2-Cys PRX redox system |
is important for |
lipid metabolism |
Arabidopsis thaliana |
| de novo fatty acid biosynthesis |
requires |
acetyl-CoA |
|
| acetate |
would have to be imported into |
chloroplast |
|
| thylakoid membranes |
harbor |
biochemical pathways for the synthesis of fatty acids (FAs) |
|
| GmLEC1c and GmLEC1d |
were unlikely to be involved in regulation of |
seed fatty acid biosynthesis |
Glycine max |
| GmLEC1a and GmLEC1b |
are involved in regulation of |
seed fatty acid biosynthesis |
Glycine max |
| lack of strong phenotypes of plastidial enolase and phosphoglycerate mutase mutants |
led to the suggestion that |
in oilseed embryos, all the 3-PGA generated in the plastid may be exported to the cytosol |
Arabidopsis thaliana |
| (AtSRT2, SRT2, AT5G09230) mutant |
shows variation in |
total fatty acid content |
Arabidopsis thaliana |
| epigenetic modulator (BGT, GCN5, HAC3, HAG01, HAG1, HAT1, AT3G54610) |
positively regulates expression of |
(AtFAD3, FAD3, AT2G29980) (LACS2, LRD2, AT1G49430) (ATLPP3, LPP3, AT3G02600) and PLAIII β |
Arabidopsis thaliana |
| (AAE13, AT3G16170) gene |
encodes |
mtAAE13 protein |
Arabidopsis thaliana |
| (FUS3, AT3G26790) overexpression |
increases expression of lower proportion of genes involved in |
fatty acid biosynthesis |
Arabidopsis thaliana |
| (AtFAP1, FAP1, AT3G63170) |
is found in |
module 2 |
Elaeis guineensis |
| palmitic acid |
is significantly higher in |
cultured pennycress embryos |
|
| histone acetyltransferase general control non-repressed protein 5 (BGT, GCN5, HAC3, HAG01, HAG1, HAT1, AT3G54610) |
modulates |
fatty acid biosynthesis |
Arabidopsis thaliana |
| (ATX2, SDG30, AT1G05830) mutant |
has total fatty acid content significantly reduced compared with |
wild-type plants |
Arabidopsis thaliana |
| lipid phosphate phosphatase 3 (ATLPP3, LPP3, AT3G02600) |
is |
downregulated gene in (BGT, GCN5, HAC3, HAG01, HAG1, HAT1, AT3G54610) mutant involved in fatty acid synthesis |
Arabidopsis thaliana |
| disruption of (BGT, GCN5, HAC3, HAG01, HAG1, HAT1, AT3G54610) |
results in moderately increased |
linoleic acid (LA, C18:2) content |
Arabidopsis thaliana |
| acetyl-CoA carboxylase |
catalyzes irreversible formation of |
malonyl CoA |
Chlorella UTEX29 |
| inhibition of the glyoxylate cycle |
leads to |
increased availability of acetyl-CoA for fatty acid synthesis |
Chlamydomonas |
| ctAAE13 protein |
is not capable of complementing |
loss of (AAE13, AT3G16170) gene function |
Arabidopsis thaliana |
| ketoacyl-ACP synthase III (KASIII) |
is absent from |
Module 1 |
Elaeis guineensis |
| SAD-1 |
might correspond to |
main SAD isoform for 18:1 production |
Elaeis guineensis |
| two novel TFs identified in the FAS subnetwork |
play important role in |
FA biosynthesis |
|
| combining different alleles carrying Gmsacpd-a, Gmsacpd-b, Gmsacpd-d, and/or soft Gmsacpd-c (group II) mutations |
may have |
additive effect resulting in dramatic increase of stearic acid content |
Glycine max |
| 35S:TPT lines |
had significantly increased |
total fatty acid content in seeds |
Arabidopsis thaliana |
| another alternative pathway for fatty acid biosynthesis |
should be considered in |
seeds |
Arabidopsis thaliana |
| fatty acid biosynthesis genes |
include |
one member of plant SACPD family proteins (GmSACPD-C) |
Glycine max |
| GmSACPD-C enzyme |
has been over-explored for use in |
producing high-stearic-acid soybeans |
Glycine max |
| mutations in GmSACPD-C |
play important role in |
nodule and leaf stearic acid content |
Glycine max |
| oleate desaturase (AtFAD2, FAD2, AT3G12120) gene |
modifications in |
biosynthesis of epoxy fatty acids |
|
| three alleles of CsFAD3 |
may explain |
higher levels of 18:3 (~35% versus ~20%) than found in diploid Arabidopsis |
Camelina sativa; Arabidopsis thaliana |
| potential dual sites for fatty acid biosynthesis |
can be proposed based on identification of |
PKS/FAS type I |
Chlorella vulgaris |
| DE genes |
were over-represented among |
genes related to fatty acid biosynthetic process, abiotic stress response, photosynthesis, integral components of membrane, and cell wall |
Zea mays |
| alternative pathways to provide precursors for fatty acid biosynthesis |
could exist |
fatty acid biosynthesis |
Arabidopsis thaliana |
| characterizations of effects of exogenous or engineered enzymes on fatty acid biosynthesis intermediates |
via |
mass spectrometry |
|
| high-stearic-acid lines |
will positively impact development of |
soybean nutritional value enhancement |
|
| Arabidopsis (ROD1, AT3G15820) mutant defective in phosphatidylcholine:diacylglycerol cholinephosphotransferase (PDCT)-mediated flux |
displayed reduced |
hydroxy fatty acid synthesis in seeds engineered for castor bean hydroxylase expression |
Arabidopsis thaliana |
| GmWRI1a expression level |
is significantly correlated to |
seed oil content in mature seeds |
Glycine max |
| acetyl-CoA |
origin in chloroplast is |
still under debate |
|
| histone acetyltransferase general control non-repressed protein 5 (BGT, GCN5, HAC3, HAG01, HAG1, HAT1, AT3G54610) |
targets |
(LACS2, LRD2, AT1G49430) |
Arabidopsis thaliana |
| (BGT, GCN5, HAC3, HAG01, HAG1, HAT1, AT3G54610) mutant |
has altered |
ratio of α-linolenic acid (ALA) to linoleic acid (LA) |
Arabidopsis thaliana |
| (ASHH3, SDG7, AT2G44150) mutant |
shows variation in |
total fatty acid content |
Arabidopsis thaliana |
| increased abundance in acetyl-CoA carboxylase (ACCase) and other fatty acid biosynthesis-related proteins upon long-term nitrogen starvation (−N) |
was observed in |
Chlorella vulgaris |
Chlorella vulgaris |
| metabolic flux study in nitrogen-starved heterotrophic Chlorella protothecoides |
suggests |
increased flux of carbon towards the synthesis of fatty acids upon nitrogen starvation (−N) |
Chlorella protothecoides |
| mitochondria |
require malonyl-CoA to support biosynthesis of |
fatty acids |
|
| plastidial and mitochondrial systems |
is |
Type II FAS systems |
|
| Module 1 |
contains |
genes involved in core fatty acid biosynthetic machinery |
Elaeis guineensis |
| (AtFaTA, FaTA, FATA1, AT3G25110) FATB1, FATB2, KASII, SAD-1, SAD-2 |
are genes likely to control |
fatty acid composition of mesocarp oil |
Elaeis guineensis |
| evolution of variations in enzymatic and nonenzymatic protein structures, stoichiometry, and protein–protein interactions |
can give rise to |
extremes in fatty acid chain lengths—short and long—as well as unique functional groups |
|
| successive condensation reactions catalysed by KAS, KAR, HD, and ENR |
yield |
C16:0- and C18:0-ACP |
Haematococcus pluvialis |
| (AtSRT2, SRT2, AT5G09230) mutant |
has total fatty acid content significantly reduced compared with |
wild-type plants |
Arabidopsis thaliana |
| (AtFAD3, FAD3, AT2G29980) |
has crucial role in |
ALA synthesis |
Arabidopsis thaliana |
| constitutive expression of (AtFAD3, FAD3, AT2G29980) in (BGT, GCN5, HAC3, HAG01, HAG1, HAT1, AT3G54610) mutant seeds |
significantly increases |
ALA content |
Arabidopsis thaliana |
| acetyl-CoA |
is substrate of |
plastidial fatty acid synthase (ptFAS) system |
|
| 18:3 at the sn-2 position of TAG in ar21 |
showed |
major reduction |
Arabidopsis thaliana |
| GO term 'fatty acid biosynthetic process' |
is significantly enriched in |
downregulated genes in spines of 1.6cm fruit |
Cucumis sativus |
| three different proteins of nuclear-encoded subunits of acetyl CoA carboxylase |
corresponding to |
four different genes ( (BCCP, BCCP-1, BCCP1, CAC1, CAC1-A, CAC1A, AT5G16390) (CAC2, AT5G35360) and (CAC3, AT2G38040) ) |
Solanum lycopersicum |
| significant up-regulation of acetyl-CoA carboxylase gene (ACCD, ATCG00500) |
reported in |
chromoplasts |
Solanum lycopersicum |
| overexpression of acyl-ACP Δ9-desaturase gene |
does not significantly change |
total fatty acid accumulation |
Phaeodactylum tricornutum |
| microalgal fatty acid synthetic process in the chloroplast |
is shown in |
Figure 8 |
Haematococcus pluvialis |
| dense shade |
inhibits |
total oil and α-ESA biosynthesis |
|
| (AtFAD2, FAD2, AT3G12120) desaturase |
catalyzes |
desaturation of oleic acid to produce linoleic acid |
|
| (AtFAD2, FAD2, AT3G12120) RNAi transgene |
increases |
oleic acid content |
Crambe abyssinica |
| reassimilation of CO2 generated from OxPPP by Rubisco |
clearly shown in |
non-photosynthetic oil-accumulating seeds |
|
| stearoyl-ACP desaturase |
is responsible enzyme for |
oleate production |
Olea europaea |
| significant decrease in several saturated fatty acids during the 2-h dark period |
could be expected since |
fatty acid biosynthesis is known to be stimulated in the light |
Arabidopsis thaliana |
| overexpression of CsaLPAT2 |
significantly increased |
C20:0 content |
Arabidopsis thaliana |
| Fatty acid biosynthetic process (GO:0006633) |
is enriched for |
GPAT5-associated translatome |
Arabidopsis thaliana |
| heteromeric acetyl-CoA carboxylase (ACCase) |
functions in |
de-novo biosynthesis of fatty acids |
|
| lipid biosynthesis capacity in tomato chromoplast |
is attested by presence of |
all proteins involved in synthesis of 3-oxoacyl-ACP |
Solanum lycopersicum |
| plastid-encoded (ACCD, ATCG00500) |
found in |
tomato chromoplast proteome |
Solanum lycopersicum |
| (ACCD, ATCG00500) |
is |
only plastid-encoded gene involved in fatty acid biosynthesis showing stable expression in chromoplasts |
Solanum lycopersicum |
| CM102G09, CM006G10, and CM032E11 |
are important for |
fatty acid biosynthesis |
Gossypium hirsutum |
| metabolic changes |
are a direct consequence of |
missing mtAAE13 function |
Arabidopsis thaliana |