| ddrm2 mutants |
are not sensitive to |
HU-induced replication stress |
|
| SA |
can trigger |
DNA damage |
Arabidopsis thaliana |
| (ANAC008, SOG1, AT1G25580) (Suppressor of Gamma Response1) |
associates with |
DDRM2 promoter |
Arabidopsis thaliana |
| DDRM2 |
may be |
functional counterpart of MDC1 |
|
| linear model |
best describes |
interaction between number of (ATRAD51, RAD51, AT5G20850) mRNAs and extent of DNA damage |
Arabidopsis thaliana |
| G2/M reporter expression |
increases with |
increasing concentrations of zeocin |
Arabidopsis thaliana |
| chronic exposure to As(III) |
can lead to |
increased DNA damage |
|
| CIP treatment |
effect is most likely due to |
DSB-inducing activity |
|
| DDRM2 |
functions downstream of |
(ANAC008, SOG1, AT1G25580) |
Arabidopsis thaliana |
| (ATRAD51, RAD51, AT5G20850) transcriptional output |
exhibits decrease in |
elongation zone compared with meristem region |
Arabidopsis thaliana |
| (ATWHY1, PTAC1, WHY1, AT1G14410) and (ATWHY3, PTAC11, WHY3, AT2G02740) mutants |
are sensitive to |
ciprofloxacin (CIP) |
Arabidopsis thaliana |
| ddrm2-1 mutant |
shows hypersensitivity to |
camptothecin (CPT) |
Arabidopsis thaliana |
| DNA damage |
can lead to |
programmed cell death (PCD) |
Arabidopsis thaliana |
| (AT4G02110) gene |
encodes |
protein with four (ATBRCA1, BRCA1, AT4G21070) C-terminal (BRCT) domains |
Arabidopsis thaliana |
| (ANAC008, SOG1, AT1G25580) (Suppressor of Gamma Response1) |
activates |
DDRM2 expression |
Arabidopsis thaliana |
| phosphorylated H2AX |
forms foci |
DNA damage foci |
|
| DNA damage response |
reveals new associations with |
cell cycle |
Arabidopsis thaliana |
| phosphorylated H2A.X histone variant (γH2AX) |
participates in |
early signaling of DNA lesion |
|
| DDRM2 |
functions downstream of |
(ANAC008, SOG1, AT1G25580) (Suppressor of Gamma Response1) |
Arabidopsis thaliana |
| zeocin |
promotes arrest at |
G1/S checkpoint |
|
| induced expression of DDRM2 |
is abolished in |
sog1-1 mutant |
Arabidopsis thaliana |
| PTIP |
contains |
BRCT domain |
|
| (ATRAD51, RAD51, AT5G20850) transcription |
is |
dose-dependent |
Arabidopsis thaliana |
| (ATRAD51, RAD51, AT5G20850) transcription |
occurs outside |
S/G2 cell cycle phases |
|
| (ATRAD51, RAD51, AT5G20850) |
shows transcriptional response to |
DNA damage |
Arabidopsis thaliana |
| stem cell death |
likely explains |
highest (ATRAD51, RAD51, AT5G20850) mRNA levels in stem cells following 10 μM zeocin treatment |
Arabidopsis thaliana |
| higher (ATRAD51, RAD51, AT5G20850) transcript output in stele cells |
is possibly explained by |
abundance of EdU-positive cells in stele |
Arabidopsis thaliana |
| E. coli ITPA mutant |
has |
induced SOS response |
Escherichia coli |
| BRCT domain |
is present in |
many DNA damage response (DDR) proteins |
|
| DDRM2 |
may have functions other than |
homologous recombination (HR) |
|
| H2AXA-YFP and DDRM2-mCherry foci |
partially overlap |
DNA damage sites |
|
| histone γH2AX levels |
correlate with |
DNA damage amounts |
|
| ddrm2-2 (ATRAD51, RAD51, AT5G20850) double mutant |
is more sensitive to |
camptothecin (CPT) |
Arabidopsis thaliana |
| greater accumulation of damage |
may underscore |
elevated transcriptional response of (ATRAD51, RAD51, AT5G20850) in stele cells |
|
| (ANAC008, SOG1, AT1G25580) |
plays crucial role in |
transcriptional regulation of DDR genes |
|
| DSBs |
induce expression of |
DDRM2 |
Arabidopsis thaliana |
| DNA damage response (DDR) activation |
leads to |
cell cycle arrest |
|
| increasing zeocin concentration |
causes drastic decline in |
number of EdU-positive cells |
Arabidopsis thaliana |
| root stele cells |
differ from other cell types in |
cell cycle changes |
|
| root stele cells |
exhibit |
more extensive (ATRAD51, RAD51, AT5G20850) transcriptional activation |
|
| (ANAC008, SOG1, AT1G25580) |
induces expression of |
DDRM2 |
|
| different sources of DNA damage |
induce |
different types of lesions |
|
| DDRM2 expression |
is induced upon |
DNA damage treatment |
Arabidopsis thaliana |
| ddrm2-2 (ATRAD51, RAD51, AT5G20850) double mutant |
is more sensitive to CPT than |
(ATRAD51, RAD51, AT5G20850) single mutant |
|
| zeocin treatment |
increases |
(ATRAD51, RAD51, AT5G20850) mRNA levels |
Arabidopsis thaliana |
| SUPPRESSOR OF GAMMA RESPONSE1 (ANAC008, SOG1, AT1G25580) |
is |
functional counterpart of p53 |
|
| DNA damage |
induces |
DNA damage response (DDR) |
Arabidopsis thaliana |
| Arabidopsis thaliana roots |
is model system for investigating |
DNA damage response |
Arabidopsis thaliana |
| (ATRAD51, RAD51, AT5G20850) transcription |
shows positive correlation with |
increasing amounts of damage |
|
| repair of double-strand breaks (DSBs) |
is critical for |
cell survival |
|
| DDRM2 |
is |
homolog of human HsTOPBP1 |
|
| CRISPR-Kill system |
relies on |
double-strand break (DSB) induction |
|
| cell cycle arrest |
provides |
necessary time for repair |
|
| stem cells |
show highest RAD51 mRNA levels following |
10 μM zeocin treatment |
Arabidopsis thaliana |
| DNA damaging agent |
induces varying |
(ATRAD51, RAD51, AT5G20850) mRNA transcriptional output |
|
| DNA damage |
may directly impact |
expression of reporter genes |
|
| G2/M-marker gene (CYC1, CYCB1, CYCB1;1, AT4G37490) |
is responsive to |
DNA damage |
|
| genomic repeated genomic DNA |
is susceptible to damage |
DNA damage |
Neurospora crassa |
| double-stranded breaks (DSBs) |
triggers |
DNA damage response (DDR) activation |
|
| differences in (ATRAD51, RAD51, AT5G20850) promoter activity |
may explain |
varying (ATRAD51, RAD51, AT5G20850) mRNA transcriptional output |
|
| camptothecin (CPT) |
causes |
double-strand breaks (DSBs) |
Arabidopsis thaliana |
| phosphorylation of H2AX |
is one of the earliest events following |
formation of double-strand breaks (DSBs) |
|
| (ATRAD51, RAD51, AT5G20850) |
can be transcribed in |
G1 phase |
|
| DSBs |
induces expression of |
DDRM2 |
Arabidopsis thaliana |
| double-strand breaks (DSBs) |
activate |
(ANAC008, SOG1, AT1G25580) |
|
| DNA damage response in plants |
demonstrates quantitative differences in |
DDR activation |
Arabidopsis thaliana |
| ddrm2-2 (ATRAD51, RAD51, AT5G20850) double mutant |
is more sensitive to CPT than |
ddrm2-2 single mutant |
|
| Arabidopsis thaliana |
encodes |
(G-H2AX, GAMMA-H2AX, H2AXA, HTA5, AT1G08880) and (G-H2AX, GAMMA-H2AX, H2AXB, HTA3, AT1G54690) |
Arabidopsis thaliana |
| G1/S and G2/M checkpoints |
can be used to ensure |
cell cycle arrest in response to DNA damage |
|
| genome |
is constantly challenged by |
genotoxic insults |
|
| histone γH2AX dynamics of recruitment and loss |
are employed to measure |
DSB repair dynamics |
|
| MDC1 |
contains |
BRCT domain |
|
| number of (ATRAD51, RAD51, AT5G20850) mRNA molecules detected per cell |
increases in response to |
increasing zeocin concentrations |
Arabidopsis thaliana |
| DDRM2 expression |
is dependent on |
(ANAC008, SOG1, AT1G25580) (Suppressor of Gamma Response1) |
Arabidopsis thaliana |
| zeocin concentration and cell type parameters |
show significant interaction in |
(ATRAD51, RAD51, AT5G20850) transcriptional response |
Arabidopsis thaliana |
| putative orthologs of 22 genes induced by genotoxic agents |
were analyzed for |
expression patterns in EBC |
Mesembryanthemum crystallinum |
| gh1-hmga1-2 mutants |
are hypersensitive to |
mitomycin C (MMC) |
Arabidopsis thaliana |
| ddrm2 mutant |
is hypersensitive to |
DSB-inducing reagents |
Arabidopsis thaliana |
| phosphorylated H2A.X histone variant (γH2AX) |
participates in |
recruitment of DNA repair machinery proteins |
|
| cells |
modulate |
(ATRAD51, RAD51, AT5G20850) transcription |
|
| hydroxyurea (HU) treatment |
activates |
G1/S checkpoint |
|
| down-regulation of (HMGA, AT1G14900) proteins |
enhances |
cancer cell sensitivity to DNA damage |
|
| cells |
undergo arrest at |
G2/M checkpoint |
|
| absence of GH1-HMGA1 |
would result in higher amounts of |
histone H1 on DNA at double-strand break (DSB) sites |
Arabidopsis thaliana |
| absence of histone H1 |
promotes |
homologous recombination (HR) |
Saccharomyces cerevisiae |
| genotoxic stress |
causes severe damage to |
nucleic acids |
|
| two genes in DNA damage response set |
were |
significantly down-regulated |
Mesembryanthemum crystallinum |
| (HMGA, AT1G14900) proteins |
have role in |
DNA damage response (DDR) process |
|
| (PARP1, AT2G31320) and (APP, PARP2, PP, AT4G02390) genes |
are up-regulated by |
DNA damage |
|
| seedlings of wild-type, main-2 and pp7l-1 |
were incubated for 2 h with |
DNA damaging drug zeocin |
Arabidopsis thaliana |
| transgenic plants harboring iMseI expression vector |
subjected to incubation at elevated temperatures show elevated expression of |
DSB-responsive genes ( (ATBRCA1, BRCA1, AT4G21070) and (ATRAD51, RAD51, AT5G20850) ) |
Arabidopsis thaliana |
| (FAS1, FUGU2, NFB2, AT1G65470) mutant |
shows |
MMS sensitivity |
Arabidopsis thaliana |
| WT or m123-2 genotypes |
show |
relatively mild effect of acute (ACPT, AtcPT3, CPT, cPT3, AT2G23410) treatment |
Arabidopsis thaliana |
| plateau of (PARP1, AT2G31320) and (ATBRCA1, BRCA1, AT4G21070) expression |
is lower in |
(FAS1, FUGU2, NFB2, AT1G65470) m123-2 than |
Arabidopsis thaliana |
| DNA-damage response (DDR) |
results in |
chromosome fusions |
|
| limiting amount of histone H1 on DNA at double-strand break (DSB) sites |
favors |
homologous recombination (HR) repair |
Arabidopsis thaliana |
| gh1-hmga1-2 mutants |
show less than 20% of plants showing |
resistance to γ-irradiation |
Arabidopsis thaliana |
| higher order chromatin organization imposed by histone H1 |
inhibits |
homologous recombination (HR) |
|
| DNA repair function of GH1-HMGA1 |
seems to be |
widely conserved in eukaryotes |
|
| Arabidopsis (CST, CX32, Kin4, PBL30, AT4G35600) subunit ( (ATCTC1, CTC1, AT4G09680) (ATSTN1, STN1, AT1G07130) or (MDO1, TEN1, AT1G56260) ) mutation |
is accompanied by |
massive genomic instability |
Arabidopsis thaliana |
| Arabidopsis (GRL, LPL3, NAP1, NAPP, AT2G35110) |
is important for |
somatic homologous recombination (HR) |
Arabidopsis thaliana |
| cell death in (PP7L, AT5G10900) mutants |
was caused by constitutive activation of |
(ANAC008, SOG1, AT1G25580) |
Arabidopsis thaliana |
| SOG1-independent cell death |
was suggested to be consequence of |
DNA repair processes not being efficiently activated |
Arabidopsis thaliana |
| (FAS1, FUGU2, NFB2, AT1G65470) m123-2 mutant |
shows ratio more similar to |
m123-2 mutant than (FAS1, FUGU2, NFB2, AT1G65470) or WT |
Arabidopsis thaliana |
| mammalian (HMGA, AT1G14900) overexpression |
sensitizes |
cancer cell lines to cisplatin |
Homo sapiens |
| DSBs-induced expression of DDRM2 |
is |
SOG1-dependent manner |
Arabidopsis thaliana |
| (ATXRCC1, XRCC1, AT1G80420) |
contains |
BRCT domain |
|
| increasing zeocin concentrations |
increases |
total number of (ATRAD51, RAD51, AT5G20850) transcripts in root meristem |
Arabidopsis thaliana |
| absence of histone H1 |
correlates with |
greater ability to repair double-strand breaks (DSB) |
Saccharomyces cerevisiae; Mus musculus |
| release of (POL, AT2G46920) II and its nascent transcript from the 3′end of highly transcribed genes |
enhances |
genome stability |
Saccharomyces cerevisiae |
| (FAS1, FUGU2, NFB2, AT1G65470) (GRL, LPL3, NAP1, NAPP, AT2G35110) ;1 ;2 ;3 quadruple mutant |
shows |
decreased sensitivity to genotoxic stress |
Arabidopsis thaliana |
| (GRL, LPL3, NAP1, NAPP, AT2G35110) |
is not required to activate |
H2A.X phosphorylation via (ATATM, ATM, ATM-1, PIG1, AT3G48190) or (ATATR, ATR, ATR-2, ATRAD3, AT5G40820) in (FAS1, FUGU2, NFB2, AT1G65470) |
Arabidopsis thaliana |
| loss of function of MAIN and its interaction partners (MAIL1, AT2G25010) and (PP7L, AT5G10900) |
leads to |
genome instability |
|
| (FAS1, FUGU2, NFB2, AT1G65470) mutant |
shows |
equal sensitivity to MMS, MMC and bleomycin |
Arabidopsis thaliana |
| (PARP1, AT2G31320) |
binds |
damaged DNA |
|
| (ABO4, EMB142, EMB2284, EMB529, ESD7, POL2A, TIL1, AT1G08260) mutants |
did not accumulate more DSBs than |
wild type |
|
| (POLQ, TEB, AT4G32700) gene |
genetically interacts with |
(ATATR, ATR, ATR-2, ATRAD3, AT5G40820) |
Arabidopsis thaliana |
| DDR signalling |
was not impaired in |
main-2 and pp7l-1 mutants |
Arabidopsis thaliana |
| typical symptoms of active DNA damage response |
includes |
precocious cell differentiation in RAM |
|
| DNA damage detection |
leads to activation of |
(ATATM, ATM, ATM-1, PIG1, AT3G48190) AND RAD3-RELATED (ATATR, ATR, ATR-2, ATRAD3, AT5G40820) |
|
| (FAS1, FUGU2, NFB2, AT1G65470) genotype |
shows |
overall highest level of damage |
Arabidopsis thaliana |
| incorrect chromatin assembly in (FAS1, FUGU2, NFB2, AT1G65470) |
is related to |
increased sensitivity to genotoxic stress |
Arabidopsis thaliana |
| six-base cutters |
do not significantly elevate |
(ATBRCA1, BRCA1, AT4G21070) expression |
Arabidopsis thaliana |
| disruption of (GRL, LPL3, NAP1, NAPP, AT2G35110) genes in (FAS1, FUGU2, NFB2, AT1G65470) |
causes |
general tolerance to genotoxic stress |
Arabidopsis thaliana |
| conserved pathways |
are activated in a similar way in |
(FAS1, FUGU2, NFB2, AT1G65470) and m123-2 |
Arabidopsis thaliana |
| severe DNA damage |
leads to |
expression of DNA replication-related and DNA repair-related genes |
Oryza sativa |
| loss of function of MAIN/ (MAIL1, AT2G25010) (PP7L, AT5G10900) complex |
was associated with |
genome instability |
Arabidopsis thaliana |
| DNA damage response (DDR) activation |
results in |
transient arrest of root growth |
Arabidopsis thaliana |
| (FAS1, FUGU2, NFB2, AT1G65470) m123-2 mutant |
shows |
about half of (ATBRCA1, BRCA1, AT4G21070) and (PARP1, AT2G31320) expression in (FAS1, FUGU2, NFB2, AT1G65470) |
Arabidopsis thaliana |
| PARP family members in humans |
are activated and recruited in response to |
single-strand and double-strand DNA breaks |
Homo sapiens |
| DNA damage |
accumulates in seeds even in the absence of |
external stresses |
|
| OsPARP1 |
is upregulated by |
bleomycin |
Oryza sativa |
| chloroplast-targeted RecA mRNA |
increases in response to |
DNA damage |
Physcomitrella patens |
| SOG1-independent cell death |
supported conclusion that |
cell death was caused by genome instability |
Arabidopsis thaliana |
| (FAS1, FUGU2, NFB2, AT1G65470) genotype |
shows |
all roots examined with small or large lesions |
Arabidopsis thaliana |
| treatment with DNA damaging agents (zeocin or bleomycin) or ionizing radiation |
leads to activation of |
DNA damage response (DDR) |
Arabidopsis thaliana |
| activation of programmed cell death (PCD) in root initials |
is an important response to |
DNA damage |
Arabidopsis thaliana |
| typical symptoms of active DNA damage response |
includes |
reduced cell division |
|
| DSB-responsive genes |
upregulated in a heat-dependent manner in |
tetraploid plants |
Arabidopsis thaliana |
| (ATATM, ATM, ATM-1, PIG1, AT3G48190) AND RAD3-RELATED (ATATR, ATR, ATR-2, ATRAD3, AT5G40820) |
phosphorylates |
SUPPRESSOR OF GAMMA-RESPONSE 1 (ANAC008, SOG1, AT1G25580) |
|
| loss of function of MAIN and its interaction partners (MAIL1, AT2G25010) and (PP7L, AT5G10900) |
leads to |
constitutive DNA damage |
|
| MseI restriction enzyme |
induces highest |
(ATBRCA1, BRCA1, AT4G21070) expression |
Arabidopsis thaliana |
| (ANAC008, SOG1, AT1G25580) |
is activated upon |
DNA damage |
|
| bleomycin treatment |
activated |
OsPARP1 expression |
Oryza sativa |
| all tested genes |
was induced in |
(POLQ, TEB, AT4G32700) single mutants and (ABO4, EMB142, EMB2284, EMB529, ESD7, POL2A, TIL1, AT1G08260) compared to wild type |
|
| Chaos1 mouse mutants |
are viable but show |
genomic instability, especially in erythrocytes |
Mus musculus |
| DNA damage |
triggers via the activity of |
(ATATR, ATR, ATR-2, ATRAD3, AT5G40820) and STEMINs |
Physcomitrella patens |
| main-2 sog1-7 double mutant |
showed more cell death than |
main-2 single mutant |
|
| (GRL, LPL3, NAP1, NAPP, AT2G35110) genes |
or act as key mediators of |
DNA damage response in (FAS1, FUGU2, NFB2, AT1G65470) |
Arabidopsis thaliana |
| repair of protein–DNA crosslinks |
occurs via |
dedicated enzyme complexes |
|
| constitutive activation of DDR |
is consistent with |
role of (POL, AT2G46920) θ in maintenance of genome integrity |
Arabidopsis thaliana |
| replicative stress induced by (POL, AT2G46920) ε deficiency |
is enhanced by |
lack of (POL, AT2G46920) θ |
|
| cells overexpressing HMGA2 |
have |
prolonged presence of DNA-PKcs at DSB sites |
|
| (FAS1, FUGU2, NFB2, AT1G65470) mutant |
shows |
four-fold higher (PARP1, AT2G31320) than WT |
Arabidopsis thaliana |
| successful DNA repair |
allows |
cell survival |
|
| cell-type-specific programmed cell death (PCD) |
is seen after 20-h treatment with |
radiometric drug zeocin |
Arabidopsis thaliana |
| (FAS1, FUGU2, NFB2, AT1G65470) m123-2 genotype |
shows |
stronger response to acute (ACPT, AtcPT3, CPT, cPT3, AT2G23410) treatment |
Arabidopsis thaliana |
| WT genotype |
indicates |
relatively mild effect of chronic (ACPT, AtcPT3, CPT, cPT3, AT2G23410) treatment |
Arabidopsis thaliana |
| all tested DDR marker genes |
were significantly induced in |
(POLQ, TEB, AT4G32700) mutants grown in the presence of salt compared to control conditions |
|
| DNA damage signalling |
is little known in |
plants |
|
| poly [ADP-ribose] polymerase 1 (PARP-1) |
plays critical role in |
genomic stability by mediating DNA damage surveillance network |
|
| ETHYLEN RESPONSE FACTOR 115 (ERF115, AT5G07310) |
showed significantly increased expression in |
pp7l-1 and pp7l-3 mutant lines compared with wild-type |
Arabidopsis thaliana |
| increased expression of DDR-related genes |
was previously shown to occur in |
root tips of main-2 and mail1-1 seedlings |
Arabidopsis thaliana |
| riboflavin deficiency |
caused |
DNA damage |
Oryza sativa |
| severe DNA damage |
may induce |
permanent cell proliferation arrest |
|
| transcription factors (anac044, NAC044, AT3G01600) and (anac085, NAC085, AT5G14490) |
are activated by |
DNA damage |
Arabidopsis thaliana |
| (FAS1, FUGU2, NFB2, AT1G65470) m123-2 |
shows lower levels of |
(ATBRCA1, BRCA1, AT4G21070) and (PARP1, AT2G31320) transcripts |
Arabidopsis thaliana |
| PARPs |
play critical roles in regulating |
DNA damage |
|
| impaired repair of replication-associated DNA damage |
could lead to |
activation of the DDR |
|
| mutations in Drosophila gene MUS308 |
induce |
hypersensitivity to replication-blocking lesions such as inter-strand cross-links |
Drosophila melanogaster |
| (ATATM, ATM, ATM-1, PIG1, AT3G48190) mutant meiocytes |
have increased |
DSB numbers |
|
| atbrca2a-1 / atbrca2b-1 double mutant |
shows additive increase in sensitivity to |
genotoxic stresses |
Arabidopsis thaliana |
| AtBRCA2a-1 –/– / AtBRCA2a-1 +/– heterozygous plants |
shows sensitivity to cisplatin same as |
atbrca2a single mutant plants |
Arabidopsis thaliana |
| expression levels of XRI-1, BRCA2, and (SMR7, AT3G27630) |
were increased approximately 1.7-fold by |
salt treatment in wild type |
|
| chilling, heavy metal, or oxidative stresses |
can cause |
DNA strand breaks |
|
| SUPPRESSOR OF GAMMA-RESPONSE 1 (ANAC008, SOG1, AT1G25580) |
induces transcriptional induction of |
cell cycle inhibitors |
|
| (ANAC008, SOG1, AT1G25580) |
induces |
cell-type-specific programmed cell death in root initials |
|
| (FAS1, FUGU2, NFB2, AT1G65470) mutant |
shows |
no further induction of (ATBRCA1, BRCA1, AT4G21070) expression after (ACPT, AtcPT3, CPT, cPT3, AT2G23410) treatment |
Arabidopsis thaliana |
| repair of protein–DNA crosslinks |
occurs via |
conserved repair machineries, such as the (ATMRE11, MRE11, AT5G54260) (ATRAD50, RAD50, AT2G31970) /NBSI complex and (ATBRCA1, BRCA1, AT4G21070) |
|
| these genes |
displayed even higher upregulation in |
(ABO4, EMB142, EMB2284, EMB529, ESD7, POL2A, TIL1, AT1G08260) (POLQ, TEB, AT4G32700) than in single mutants |
|
| atbrca2a-1 single mutant |
is hypersensitive to |
cisplatin |
Arabidopsis thaliana |
| Deficiency in non-replicative polymerases |
results in |
hypersensitivity to various DNA-damaging agents |
|
| DNA single-strand breaks (SSBs) |
trigger |
complex cascade of events involving (PARP1, AT2G31320) |
|
| reactive carbonyls |
form |
genotoxic DNA–protein cross-links |
Arabidopsis thaliana |
| energy-rich radiation |
exerts |
chromatin changes |
|
| repair of DNA strand breaks |
requires |
(ATATR, ATR, ATR-2, ATRAD3, AT5G40820) kinase |
Physcomitrella patens |
| programmed cell death (PCD) in root initials |
prevents |
accumulation and propagation of deleterious mutations |
|
| homologous recombination-mediated DNA repair pathway |
might not be activated in |
pp7l-1 and pp7l-3 mutant lines |
Arabidopsis thaliana |
| (FAS1, FUGU2, NFB2, AT1G65470) m123-2 mutant |
shows |
higher proportion of PI-stained roots than WT |
Arabidopsis thaliana |
| (ATATM, ATM, ATM-1, PIG1, AT3G48190) kinase deletion in (FAS1, FUGU2, NFB2, AT1G65470) mutant |
causes |
expression of (ATBRCA1, BRCA1, AT4G21070) and (PARP1, AT2G31320) returns to wild-type levels |
Arabidopsis thaliana |
| low UV-C doses |
produced |
limited amounts of topo I covalent complexes (TCCs) |
Homo sapiens |
| large-scale decondensation of the chromatin |
could promote |
DNA damage as a response to substances occurring in enzyme solution for cell wall degradation |
|
| DNA lesions |
are recognized and trigger |
DNA damage response (DDR) |
|
| (ABO4, EMB142, EMB2284, EMB529, ESD7, POL2A, TIL1, AT1G08260) (POLQ, TEB, AT4G32700) double mutants |
accumulated significantly higher levels of |
γ-H2AX foci |
|
| consequences of salinity on DNA |
have been |
less explored |
|
| DDR gene activation |
did not differ significantly between |
small and big (POLQ, TEB, AT4G32700) mutants |
|
| loss of (POL, AT2G46920) θ |
results in |
increase in DNA damage accumulation in proliferating cells |
|
| consequences of drought on DNA |
have been |
less explored |
|
| DNA damage |
occurs in |
cell nuclei |
|
| topoisomerase I (topo I) |
shows temporal accumulation at |
damaged sites |
|
| (FAS1, FUGU2, NFB2, AT1G65470) mutant |
shows |
increased sensitivity to MMS |
Arabidopsis thaliana |
| constitutive activation of DNA repair |
occurs in |
both (FAS1, FUGU2, NFB2, AT1G65470) and m123-2 mutants |
Arabidopsis thaliana |
| DNA double-strand breaks |
trigger |
complex cascade of events involving (PARP1, AT2G31320) |
|
| DNA lesions |
activate |
ATAXIA TELANGIECTASIA MUTATED (ATATM, ATM, ATM-1, PIG1, AT3G48190) target genes |
Arabidopsis thaliana |
| WT genotype |
shows |
50% of roots remaining intact after 6 days of (ACPT, AtcPT3, CPT, cPT3, AT2G23410) treatment |
Arabidopsis thaliana |
| (FAS1, FUGU2, NFB2, AT1G65470) and (FAS2, MUB3.9, NFB01, NFB1, AT5G64630) mutants |
show |
increased expression levels of (ATRAD51, RAD51, AT5G20850) |
Arabidopsis thaliana |
| (ATBRCA1, BRCA1, AT4G21070) and (PARP1, AT2G31320) expression in (FAS1, FUGU2, NFB2, AT1G65470) m123-2 |
is higher than in |
wild-type |
Arabidopsis thaliana |
| consequences of light intensity on DNA |
have been |
less explored |
|
| chloroplast-targeted RecA mRNA |
increases in response to |
DNA damage |
pea |
| chloroplast chromosomes transmitted to next generation |
might sustain less damage than |
cpDNA in green cells |
Arabidopsis thaliana |
| mutants of genes associated with DNA metabolism |
are sensitive to |
DNA mutagens |
|
| UV-light |
is |
efficient inducer of formation of topo I-DNA complexes |
|
| fragmentation of cpDNA molecules in cprecA mutants |
increased only slightly after treatment with |
ciprofloxacin |
Arabidopsis thaliana |
| ectopically expressed marker (APP, PARP2, PP, AT4G02390) |
is not supported by |
present transcriptome analysis of (ATTOP6B, BIN3, HLQ, HYP6, RHL3, TOP6B, AT3G20780) |
Arabidopsis thaliana |
| GSTs and superoxide dismutase |
protect |
DNA against radiation |
|
| (MOM, MOM1, AT1G08060) mutants |
do not show hypersensitivity to |
DNA damage agent MMS |
Arabidopsis thaliana |
| response of (ATATR, ATR, ATR-2, ATRAD3, AT5G40820) loss-of-function mutants to Al |
is opposite to |
response of (ATATR, ATR, ATR-2, ATRAD3, AT5G40820) loss-of-function mutants to hydroxurea or ionizing radiation |
Arabidopsis thaliana |
| molecular integrity of branched cpDNA |
was greatly reduced in |
wt plants treated with ciprofloxacin |
Arabidopsis thaliana |
| hydrogen peroxide |
is |
efficient inducer of formation of topo I-DNA complexes |
|
| (POL, AT2G46920) θ deficiency |
results in |
dramatic increase of tumorigenesis upon UV exposure |
Homo sapiens |
| chloroplast-targeted RecA mRNA |
increases in response to |
DNA damage |
Chlamydomonas reinhardtii |
| chloroplast-targeted RecA mRNA |
increases in response to |
DNA damage |
Arabidopsis thaliana |
| γ-irradiation (600 Gy) |
accelerates |
stem fasciation phenotype |
Arabidopsis thaliana |
| AtCYCB1;1 induction in response to cisplatin in atbrca2a-1/atbrca2b-1 double mutant |
is compared to |
AtCYCB1;1 induction in wild-type plants |
Arabidopsis thaliana |
| Ataxia telangiectasia mutated related (ATATR, ATR, ATR-2, ATRAD3, AT5G40820) -related pathway |
could actively slow |
root growth |
|
| OsPARP1 |
respond to |
DNA damage |
Oryza sativa |
| PARP, (ATATR, ATR, ATR-2, ATRAD3, AT5G40820) (ATRAD51C, RAD51C, AT2G45280) and RAD51A2 |
have upregulated transcript levels in |
(ELL1, FK, HYD2, AT3G52940) plants |
Oryza sativa |
| staurosporine |
is |
efficient inducer of formation of topo I-DNA complexes |
|
| homozygous BRCA2 mutant mice |
show |
chromosomal rearrangements and breaks |
Mus musculus |
| rpa2-4 mutant |
is extremely sensitive to |
methyl methanesulfonate (MMS) |
Arabidopsis thaliana |
| (ATATM, ATM, ATM-1, PIG1, AT3G48190) kinase |
is the major kinase that phosphorylates |
(h2a.w.7, HTA7, AT5G27670) and H2A.X |
Arabidopsis thaliana |
| (h2a.w.7, HTA7, AT5G27670) |
performs |
unique function in heterochromatin-associated DNA damage response (DDR) |
Arabidopsis thaliana |
| ATM-dependent phosphorylation of HP1 and KAP1 |
facilitates |
double-strand break (DSB) repair |
mammals |
| γ-irradiation |
increases ratio of plants displaying |
fasciation and abnormal phyllotaxy phenotypes |
Arabidopsis thaliana |
| cells |
respond to DNA lesions by activation of |
intricate web of signalling pathways |
|
| Nossen ecotype |
is |
one of ecotypes with relatively higher sensitivity to γ-irradiation |
Arabidopsis thaliana |
| SQEF motif of Arabidopsis H2A.X |
is phosphorylated in response to |
DNA damage |
Arabidopsis thaliana |
| application of bleomycin |
may further induce |
DNA damage at M phase in CDKB2;1 knockdown callus |
|
| AtBRCA2 mutation |
enhances |
AtCYCB1;1 induction by cisplatin |
Arabidopsis thaliana |
| AtCYCB1;1 |
is strongly induced by |
DNA damaging agents |
Arabidopsis thaliana |
| extrinsic DSBs and inefficient repair of DSBs |
induce |
abnormal morphological phenotype |
Arabidopsis thaliana |
| disruption of TgPCNA2 gene |
did not influence |
response to chemical mutagens |
Toxoplasma gondii |
| atbrca2a-1 / atbrca2b-1 double mutant |
shows additive increase in sensitivity to |
genotoxic stresses |
Arabidopsis thaliana |
| transcription machinery |
may play important role in |
sensing DNA damage and activating DNA repair and stress response pathways |
Synechocystis sp. PCC 6803 |
| ros1rpa2-1 mutant |
is extremely sensitive to |
methyl methanesulfonate (MMS) |
Arabidopsis thaliana |
| wild-type plants |
are tolerant to |
methyl methanesulfonate (MMS) |
Arabidopsis thaliana |
| genes presenting type III variability |
have likely functions that are |
diverse |
|
| (ANAC008, SOG1, AT1G25580) |
functions in |
DNA damage signalling response |
Arabidopsis thaliana |
| atbrca2b-1 single mutant |
is hypersensitive to |
γ-irradiation |
Arabidopsis thaliana |
| atbrca2a-1 / atbrca2b-1 double mutant |
shows additive increase in sensitivity to |
genotoxic stresses |
Arabidopsis thaliana |
| UV-C and γ-rays |
cause |
wide variety of DNA breaks and damage |
Arabidopsis thaliana |
| ros1rpa2-1 mutant |
shows no difference to |
UV stress |
Arabidopsis thaliana |
| (ATATR, ATR, ATR-2, ATRAD3, AT5G40820) |
encodes |
ATAXIA TELANGIECTASIA-MUTATED AND RAD3-RELATED (ATATR, ATR, ATR-2, ATRAD3, AT5G40820) |
Arabidopsis thaliana |
| plants |
may have evolved |
histone variants combining the properties of H2A.X and H2A.W |
plants |
| DNA double-strand breaks (DSBs) |
enhance |
endoreduplication |
Arabidopsis thaliana |
| plants |
use endoreduplication as |
bypass pathway to avoid cell-cycle arrest or cell death by DNA damage |
|
| G 2 arrest or inhibition of mitosis |
is not |
major DNA damage response in rice |
|
| (ANAC008, SOG1, AT1G25580) |
functions downstream of |
(ATATM, ATM, ATM-1, PIG1, AT3G48190) and (ATATR, ATR, ATR-2, ATRAD3, AT5G40820) |
|
| genetic screening for DNA damage response mutants (DDRMs) |
found |
ddrm2 mutant |
Arabidopsis thaliana |
| DNA damage |
activates |
(ATRAD51, RAD51, AT5G20850) transcription |
|
| G1 cells |
increase in amount with |
increasing concentrations of zeocin |
Arabidopsis thaliana |
| DDRM2-YFP |
forms foci in |
approximately 90% of cells when treated with bleomycin (BLM) |
Arabidopsis thaliana |
| number of (ATRAD51, RAD51, AT5G20850) mRNA molecules per cell |
shows positive correlation with |
γH2AX levels |
Arabidopsis thaliana |
| differences in DNA damage sensitivity |
may explain |
varying (ATRAD51, RAD51, AT5G20850) mRNA transcriptional output |
|
| TOPBP1 |
contains |
BRCT domain |
|
| double-stranded breaks (DSBs) |
requires |
immediate repair |
|
| DNA strand distortions caused by photodimers |
block |
transcription and replication |
|
| (ANAC008, SOG1, AT1G25580) (SUPPRESSOR OF GAMMA RESPONSE 1) |
appears to function in |
responses to UV-B-induced DNA damage |
Arabidopsis thaliana |
| ataxia telangiectasia mutated (ATATM, ATM, ATM-1, PIG1, AT3G48190) kinase |
phosphorylates |
H2A.X histone variant |
|
| function of cell cycle-related factors in the DNA damage response in plants |
is not clear |
in plants |
|
| double-strand breaks (DSBs) |
is |
most threatening type of DNA damage in living cells |
|
| DNA lesions |
are rapidly detected in |
cells |
|
| gamma irradiation |
induces |
(ATRAD54, CHR25, RAD54, AT3G19210) foci emergence |
Arabidopsis |
| BRCT domain of MDC1 |
is the major mediator for recognition of |
phosphorylated H2AX |
|
| cells with no EdU signal |
contain |
abundant number of (ATRAD51, RAD51, AT5G20850) mRNA molecules |
Arabidopsis thaliana |
| increase in G1 cells |
indicates potential |
cell cycle arrest at G1/S checkpoint |
Arabidopsis thaliana |
| upregulation of DDR genes |
is |
key element of DDR response |
|
| γH2AX foci in rfc1–2 mutant |
decrease slowly and more remain at |
pachytene stage |
Arabidopsis thaliana |
| plants |
do not display |
arrest in prophase I or apoptosis in response to unrepaired DSBs |
|
| γH2AX foci in rfc1–2 meiocytes |
were maintained at |
relatively high level |
Arabidopsis thaliana |
| anthers of ctf7-1 plants |
contained elevated transcript levels for |
(ATATR, ATR, ATR-2, ATRAD3, AT5G40820) kinase |
Arabidopsis thaliana |
| TUNEL-positive signal in (MAIL1, AT2G25010) mutant root meristem |
may be |
direct consequence of unstable and damaged DNA or result from programmed cell death |
|
| (ATRAD51, RAD51, AT5G20850) transcription in response to DNA damage |
is coincident with |
cell cycle arrest at G2/M checkpoint |
Arabidopsis |
| (SMR5, AT1G07500) |
control |
cell cycle arrest in response to genotoxic stress |
Arabidopsis thaliana |
| DDRM2 |
is involved in |
both non-homologous end joining (NHEJ) and homologous recombination (HR) |
Arabidopsis thaliana |
| shorter cell cycle duration |
may explain |
elevated (ATRAD51, RAD51, AT5G20850) transcriptional response in stele cells |
|
| sog1-1 mutation |
reversed |
UV-B-hypersensitive phenotype of xpf |
Arabidopsis thaliana |
| genes involved in damage sensing ( (RBB1, AT5G40450) (CIPK3, SnRK3.17, AT2G26980) (AtCPK21, CPK21, AT4G04720) ) |
were upregulated by |
low CO2 |
Arabidopsis thaliana |
| (ANAC008, SOG1, AT1G25580) (SUPPRESSOR OF GAMMA RESPONSE 1) |
is responsible for |
expression of several genes induced after γ-irradiation |
Arabidopsis thaliana |
| histones, ubiquitin-related genes, and DNA-binding proteins |
are higher in BQC compared to |
good-quality cork (GQC) |
Quercus suber |
| (ATRPA2, ATRPA32A, ROR1, RPA2, RPA32A, AT2G24490) mutants |
are hypersensitive to |
methyl methanesulphonate |
Arabidopsis thaliana |
| two histone variants H2A.X and (h2a.w.7, HTA7, AT5G27670) |
function in parallel to |
protect genome integrity |
Arabidopsis thaliana |
| (ANAC008, SOG1, AT1G25580) (SUPPRESSOR OF GAMMA RESPONSE 1) |
has been shown to process |
signals associated with multiple responses to DNA damage |
|
| Arabidopsis thaliana mutants of the photolyases and NER enzymes |
are hypersensitive when irradiated with |
UV-B or UV-C |
Arabidopsis thaliana |
| SQEF motif of Arabidopsis H2A.X phosphorylation |
suggests conservation of |
H2A.X function in DNA damage response (DDR) |
Arabidopsis thaliana |
| bleomycin and zeocin treatment |
strongly induced transcription of |
DNA damage response genes (ATRAD51, RAD51, AT5G20850) (ATBRCA1, BRCA1, AT4G21070) and (CYC1, CYCB1, CYCB1;1, AT4G37490) |
Arabidopsis thaliana |
| H2A.X and (h2a.w.7, HTA7, AT5G27670) |
act in parallel in |
DNA damage response (DDR) |
Arabidopsis thaliana |
| (FAS1, FUGU2, NFB2, AT1G65470) mutants |
are hypersensitive to |
DNA damage agent MMS |
Arabidopsis thaliana |
| CDKB2 |
functions differently in response to |
DNA damage |
Arabidopsis thaliana; Oryza sativa |
| ectopically expressed marker (ATRAD51, RAD51, AT5G20850) |
is not supported by |
present transcriptome analysis of (ATTOP6B, BIN3, HLQ, HYP6, RHL3, TOP6B, AT3G20780) |
Arabidopsis thaliana |
| genotoxic agents (such as H2O2) |
treatment with causes |
DNA lesions in mitochondrial DNA (mtDNA) |
|
| mutations in C-terminal acetyltransferase domain of (AtCTF7, CTF7, ECO1, AT4G31400) /Esco2 |
increase |
sensitivity to DNA-damaging agents |
Saccharomyces cerevisiae; Mus musculus; Homo sapiens |
| TUNEL assay in (MAIL1, AT2G25010) mutant |
found |
high number of TUNEL-positive nuclei in mutant but not in wild-type |
|
| UV-B light (280–320nm) |
causes damage to |
biomolecules such as DNA |
|
| HMGA1 and HMGA2 positively regulating (ATATM, ATM, ATM-1, PIG1, AT3G48190) expression |
occurs via |
positive feedback loop in response to DNA damage |
|
| mammalian (HMGA, AT1G14900) overexpression |
sensitizes |
cancer cell lines to methyl-methanesulfonate |
Homo sapiens |
| higher expression of histones, ubiquitin-related genes, and DNA-binding proteins in BQC |
is |
consequence of indirect effect of damaged DNA caused by UV-B radiation |
Quercus suber |
| MCM and (AGD10, MEE28, RPA, AT2G35210) proteins |
have been shown to play roles in |
triggering DNA damage repair mechanisms in response to stalled replication forks |
|
| (ATATR, ATR, ATR-2, ATRAD3, AT5G40820) |
responds to |
replication fork stall |
|
| partial depletion of H2A.X.3 and H2A.X.5 by artificial miRNA |
resulted in |
weak sensitivity to DNA damage |
Arabidopsis thaliana |
| all three histone variants (H2A.X.3, H2A.X.5, (h2a.w.7, HTA7, AT5G27670) ) |
act within |
the same genetic pathway |
Arabidopsis thaliana |
| γH2A.X foci |
decorate |
sites of double-strand breaks (DSBs) |
Arabidopsis thaliana; metazoa |
| DNA damage |
leads to degradation of |
CDKB2 |
Arabidopsis thaliana |
| profuse production of root hairs in X-ray-irradiated B2RNAi-7 plants |
is |
typical morphological phenotypic response to DNA damage in roots |
|
| UV-B photodimers |
can activate |
DNA damage response pathways |
|
| regulating cell cycle progress |
might be |
protective mechanism to prevent cells with damaged DNA from dividing |
|
| GH1-HMGA1 mutants |
have higher sensitivity to |
mitomycin C |
Arabidopsis thaliana |
| absence of GH1-HMGA1 |
led to |
genomic instability |
Arabidopsis thaliana |
| UV light exposure |
causes |
genotoxic stress |
|
| (SMR5, AT1G07500) and (SMR7, AT3G27630) expression |
is induced under |
various DNA-damaging conditions |
Arabidopsis thaliana |
| 53BP1 |
contains |
BRCT domain |
|
| CRISPR-Kill |
relies on induction of |
multiple double-strand breaks (DSBs) |
|
| root cells |
exhibit substantial variability in |
sensitivity to DSBs induced by zeocin |
Arabidopsis thaliana |
| (APP, PARP2, PP, AT4G02390) |
is upregulated in |
m56-1fas2-4 mutant |
Arabidopsis thaliana |
| bleomycin treatment |
induced |
γ-H2A.X elevation |
Arabidopsis thaliana |
| root and shoot SCN |
may have |
distinct protection to DNA damage |
Arabidopsis thaliana |
| (ATXRCC3, XRCC3, AT5G57450) mutant |
showed greater sensitivity to |
bleomycin (BLM) |
Oryza sativa |
| RAD51A1 and RAD51A2 |
have no effect on the protein loading of |
(ATRAD51C, RAD51C, AT2G45280) and (ATXRCC3, XRCC3, AT5G57450) |
Oryza sativa |
| (ARLIM15, ATDMC1, DMC1, AT3G22880) |
cannot load at |
irradiation-induced double-strand breaks (DSBs) in rice mitotic nuclei |
Oryza sativa |
| SUPPRESSOR OF GAMMA RESPONSE 1 (ANAC008, SOG1, AT1G25580) |
binds to the promoters of |
B1-type cyclin (CYC1, CYCB1, CYCB1;1, AT4G37490) |
Arabidopsis thaliana |
| high concentration of Boron (B) |
induces |
DNA double-stranded breaks |
|
| cadmium |
induces |
genome instability |
|
| Col-0 and (AtTK1b, TK1b, TK2, AT5G23070) mutant seedlings challenged with ciprofloxacin in the absence/presence of sucrose, glucose or fructose |
tested |
effects of sugars in response to genotoxic agents |
Arabidopsis thaliana |
| (AtTK1a, TK1, TK1a, AT3G07800) promoter induction by HU or Zeocin™ |
is consistent with |
hypersensitivity shown by (AtTK1a, TK1, TK1a, AT3G07800) mutants to these genotoxins |
Arabidopsis thaliana |
| (ATRAD51, RAD51, AT5G20850) |
plays key role in |
DNA damage response |
|
| m56-1fas2-4 mutant root tip |
showed clearly detectable immunostaining signals of |
γ-H2A.X |
Arabidopsis thaliana |
| DSBs induced by I- Sce I nuclease |
only a portion was repaired by |
synthesis-dependent strand annealing (SDSA) |
Oryza sativa |
| (ATRAD51, RAD51, AT5G20850) paralogs |
have been shown to function in |
homologous recombination (HR) |
|
| (ATRAD51, RAD51, AT5G20850) and (ARLIM15, ATDMC1, DMC1, AT3G22880) |
are |
two Escherichia coli RecA homologs found in yeast, mice, and humans |
|
| SUPPRESSOR OF GAMMA RESPONSE 1 (ANAC008, SOG1, AT1G25580) |
upregulates expression of |
(SMR7, AT3G27630) |
Arabidopsis thaliana |
| (anac103, NAC103, AT5G64060) mutants |
suppress |
root growth inhibition |
Arabidopsis thaliana |
| quantitative shotgun phosphoproteomics |
provided |
high-throughput analysis of the DNA damage response network in callus cells |
|
| (AtTK1a, TK1, TK1a, AT3G07800) and (AtTK1b, TK1b, TK2, AT5G23070) |
are required for |
appropriate response to different kinds of genotoxic stress |
Arabidopsis thaliana |
| diRNA production |
is dependent on |
(ATATR, ATR, ATR-2, ATRAD3, AT5G40820) (Ataxia Telangiectasia and Rad3-related) |
Arabidopsis thaliana |
| (ATNBS1, NBS1, AT3G02680) |
plays key role in |
DNA damage response |
|
| predominant localization of γH2A.W.7 in heterochromatin and γH2A.X in euchromatin |
affects |
dynamics of dephosphorylation and turnover in DNA damage response (DDR) |
Arabidopsis thaliana |
| functional conservation of H2A.X |
is shown in |
Arabidopsis |
Arabidopsis thaliana |
| (h2a.w.7, HTA7, AT5G27670) histone variant |
plays a role similar to |
KAP1 and HP1 phosphorylation in mammals |
Arabidopsis thaliana |
| γH2A.W.7 foci associated with chromocenters after 2-hr bleomycin treatment |
represent |
13.8% of total γH2A.W.7 foci |
Arabidopsis thaliana |
| (h2a.w.7, HTA7, AT5G27670) phosphorylation |
is phosphorylated with slower dynamics than |
H2A.X phosphorylation |
Arabidopsis thaliana |
| histone variants combining the properties of H2A.X and H2A.W in plants |
facilitate |
heterochromatin accessibility during DNA damage response (DDR) |
plants |
| DNA damage responses |
are mediated by |
highly conserved (ATATM, ATM, ATM-1, PIG1, AT3G48190) and (ATATR, ATR, ATR-2, ATRAD3, AT5G40820) protein kinases |
|
| γH2A.X levels in (h2a.w.7, HTA7, AT5G27670) mutant |
decreased with slower dynamics than in |
WT |
Arabidopsis thaliana |
| photodimer formation |
is |
specific to UV-B light |
Arabidopsis thaliana |
| role of H2A.X in response to DNA damage |
is conserved in |
Arabidopsis |
Arabidopsis thaliana |
| overall patterns of (h2a.w.7, HTA7, AT5G27670) and H2A.X |
did not change upon |
DNA damage induction |
Arabidopsis thaliana |
| h2a.x mutants |
showed same dynamics of |
γH2A.W.7 foci as in WT |
Arabidopsis thaliana |
| proportion of γH2A.X foci associated with chromocenters in (h2a.w.7, HTA7, AT5G27670) mutant |
increased significantly compared to |
WT |
Arabidopsis thaliana |
| significant increase of γH2A.X foci within the chromocenters in (h2a.w.7, HTA7, AT5G27670) mutant |
correlates with |
slower dynamics of γH2A.X dephosphorylation |
Arabidopsis thaliana |
| Oryza sativa |
may enhance |
DNA repair capacity upon genotoxic stress |
Oryza sativa |
| OsRAD51A2 transcription |
is increased by |
5 Gy X-ray irradiation |
|
| DSBs |
are required for |
normal localization of (AtRFC1, RFC1, AT5G22010) to meiotic chromosomes |
Arabidopsis thaliana |
| (ATATM, ATM, ATM-1, PIG1, AT3G48190) /ATR-dependent DNA damage response activation |
presumably leads to |
halt of endoreduplication |
Arabidopsis thaliana |
| homologous recombination (HR) |
is initiated by |
DNA double-strand breaks (DSBs) |
|
| γ-irradiation |
induces |
double-strand breaks |
Arabidopsis thaliana |
| versatile approach |
will contribute to improving |
studies of DNA damage in plants |
Arabidopsis thaliana |
| levels of γH2A.X |
were not altered in |
(h2a.w.7, HTA7, AT5G27670) mutants compared to WT |
Arabidopsis thaliana |
| SQ phosphorylation in (h2a.w.7, HTA7, AT5G27670) |
may antagonize |
chromatin condensation |
Arabidopsis thaliana |
| role of (h2a.w.7, HTA7, AT5G27670) in DNA damage response (DDR) in Arabidopsis |
is parallel to |
role of heterochromatic proteins HP1 and KAP1 in mammals |
Arabidopsis thaliana; mammals |
| CDKB2 |
functions differently in |
Arabidopsis thaliana and Oryza sativa |
Arabidopsis thaliana; Oryza sativa |
| DNA damage |
neither induces |
endoreduplication |
Oryza sativa |
| transcription of RAD51A2 |
is enhanced by |
DNA damage |
|
| bleomycin-treated calli |
support conclusion that |
endoreduplication is not a major DNA damage response in rice |
|
| phosphorylated H2AX (γH2AX) |
may be regarded as marker to detect |
double-strand breaks (DSBs) |
Arabidopsis thaliana |
| (ATATR, ATR, ATR-2, ATRAD3, AT5G40820) mutants |
are hypersensitive to |
replication-blocking agents |
Arabidopsis thaliana |
| histone variant H2A.X phosphorylation |
results in |
γ-H2A.X |
Arabidopsis thaliana |
| γ-H2AX focus formation in (ATRAD51, RAD51, AT5G20850) paralog mutants |
showed no significant decrease compared with |
(ATXRCC3, XRCC3, AT5G57450) wild-type |
Oryza sativa |
| (ATXRCC3, XRCC3, AT5G57450) immune signals |
are dramatically reduced following |
wortmannin treatment |
Oryza sativa |
| SUPPRESSOR OF GAMMA RESPONSE 1 (ANAC008, SOG1, AT1G25580) |
plays a crucial role in DNA damage response as |
tumor suppressor protein p53 |
|
| CHK1 |
phosphorylates |
tumor suppressor protein p53 |
|
| (anac103, NAC103, AT5G64060) knockout (KO) lines |
showed significantly less sensitive |
root cell death phenotype |
Arabidopsis thaliana |
| NAC103-GFP overexpressing (NAC103-OE) lines |
showed commonly and significantly enhanced transcription levels of |
(ATBRCA1, BRCA1, AT4G21070) |
Arabidopsis thaliana |
| NAC103-OE-in-sog1-1 lines |
demonstrated partial, but significant, complemented expression of |
(ATBRCA1, BRCA1, AT4G21070) |
Arabidopsis thaliana |
| Overexpression of (anac103, NAC103, AT5G64060) in WT |
significantly increased |
zeocin-induced true leaf inhibition |
Arabidopsis thaliana |
| ATM-dependent protein phosphorylation |
establishes functional significance of |
plant DNA damage response |
Arabidopsis thaliana |
| (ATRBR1, RB, RB1, RBR, RBR1, AT3G12280) |
localized to |
γ-H2AX foci |
Arabidopsis thaliana |
| MIM396 transgenic plants |
accumulated lower CPDs after UV-B treatment |
compared with WT and E2FcRNAi plants |
Arabidopsis thaliana |
| GUS activity driven by the (AtTK1b, TK1b, TK2, AT5G23070) promoter |
remained unchanged with |
all treatments tested |
Arabidopsis thaliana |
| DNA double-strand breaks (DSBs) |
induce selective cell death in |
stem cells |
Arabidopsis thaliana |
| rfc1–2 mutant |
exhibits |
>50 γH2AX foci during leptotene |
Arabidopsis thaliana |
| Ddc1 protein |
requires DSBs for |
localization to meiotic chromosomes |
|
| human (ATATR, ATR, ATR-2, ATRAD3, AT5G40820) |
binds to |
UV-damaged DNA |
Homo sapiens |
| (ATATR, ATR, ATR-2, ATRAD3, AT5G40820) mutant |
regulate CYCB1;1 at the transcriptional level in |
root tips |
Arabidopsis thaliana |
| Reducing reactive oxygen species (ROS) |
protects |
genomic integrity |
|
| (ATATM, ATM, ATM-1, PIG1, AT3G48190) (Ataxia Telangiectasia Mutated) |
is |
ATAXIA TELANGIECTASIA MUTATED |
|
| reduced expression of AtRad52-2 |
had |
sensitivity to Mitomycin C |
Arabidopsis thaliana |
| γ-irradiation |
induces |
loading of (ATRAD51B, RAD51B, AT2G28560) at DSBs |
Oryza sativa |
| DNA-damage-triggered phosphorylation of (ANAC008, SOG1, AT1G25580) |
is required for |
efficient binding of (ANAC008, SOG1, AT1G25580) to target sites |
Arabidopsis thaliana |
| CTT(N)7AAG motif |
is crucial for |
DNA damage-induced expression of (ANAC008, SOG1, AT1G25580) target genes in planta |
Arabidopsis thaliana |
| (anac103, NAC103, AT5G64060) expression |
was less strongly induced by |
MMS |
Arabidopsis thaliana |
| NAC103-GFP overexpressing (NAC103-OE) lines |
showed commonly and significantly enhanced transcription levels of |
(RPA1E, AT4G19130) |
Arabidopsis thaliana |
| overexpression of (anac103, NAC103, AT5G64060) |
did not increase the transcription levels of |
(AT4G22960) |
Arabidopsis thaliana |
| NAC103-OE-in-sog1-1 lines |
demonstrated partial, but significant, complemented expression of |
(AT4G22960) |
Arabidopsis thaliana |
| Overexpression of (anac103, NAC103, AT5G64060) in WT |
significantly increased |
zeocin-induced root growth inhibition |
Arabidopsis thaliana |
| (anac103, NAC103, AT5G64060) mutants |
displayed incomplete, but significant, inhibition in transcriptional induction of |
(ATBRCA1, BRCA1, AT4G21070) |
Arabidopsis thaliana |
| H2AX-silenced lines |
displayed |
mild hypersensitivity to bleomycin |
Arabidopsis thaliana |
| gamma-irradiated mature Arabidopsis plants |
were used in |
phosphoproteomic study |
Arabidopsis thaliana |
| (ATATM, ATM, ATM-1, PIG1, AT3G48190) post-translational signalling networks |
operating in |
seeds |
Arabidopsis thaliana |
| (ATATM, ATM, ATM-1, PIG1, AT3G48190) (ataxia telangiectasia mutated) and (ATATR, ATR, ATR-2, ATRAD3, AT5G40820) ( and (RAD3, AT2G05635) related) |
are involved in perception of |
double-strand breaks and single-stranded DNA |
|
| (AtTK1a, TK1, TK1a, AT3G07800) and (AtTK1b, TK1b, TK2, AT5G23070) |
differ by |
their regulation in response to DNA damage |
Arabidopsis thaliana |
| decrease in auxin signaling |
is one of the causes of |
stem cell death |
Arabidopsis thaliana |
| DNA crosslinks |
is one of |
types of DNA damage tested |
Chlamydomonas reinhardtii |
| alt1-1 and alt1-2 mutants |
root growth is affected similarly to |
(ATATR, ATR, ATR-2, ATRAD3, AT5G40820) mutant after treatment with hydroxurea |
Arabidopsis thaliana |
| (h2a.w.7, HTA7, AT5G27670) mutant expressing S135A mutant |
did not complement |
sensitivity of (h2a.w.7, HTA7, AT5G27670) to DNA damage |
Arabidopsis thaliana |
| ATM-dependent phosphorylation of histone variant (h2a.w.7, HTA7, AT5G27670) |
is required for |
DNA damage response (DDR) |
Arabidopsis thaliana |
| DNA damage |
does not down-regulate |
Oryza sativa CDKB2;1 |
Oryza sativa |
| X-ray exposure |
increases |
DNA damage |
|
| catalytic activities of CDKs |
play |
critical role in the DNA damage response |
|
| ATAXIA-TELANGIECTASIA MUTATED (ATATM, ATM, ATM-1, PIG1, AT3G48190) |
is involved in |
plant response to DNA damage |
Arabidopsis thaliana |
| (ATATM, ATM, ATM-1, PIG1, AT3G48190) |
has a crucial role in |
H2A.X phosphorylation and DSB repair |
Arabidopsis thaliana |
| γ-irradiation |
induces |
loading of (ATXRCC3, XRCC3, AT5G57450) at DSBs |
Oryza sativa |
| recombination vectors (pSSA.I– Sce I and pSDSA.I– Sce I) |
contain |
recombination substrate |
Oryza sativa |
| (ATSUS2, SSA, SUS2, AT5G49190) repairs in (ATRAD51D, RAD51D, SSN1, AT1G07745) mutant |
were greatly compromised |
(ATRAD51D, RAD51D, SSN1, AT1G07745) mutant |
Oryza sativa |
| wortmannin |
inhibits |
plant (ATATM, ATM, ATM-1, PIG1, AT3G48190) and/or other upstream PI3K-like kinases involved in the DNA damage response |
Oryza sativa |
| (ATRAD51B, RAD51B, AT2G28560) focus formation in (ATXRCC3, XRCC3, AT5G57450) and (ATXRCC2, XRCC2, AT5G64520) mutant plants |
displayed normally compared with |
(ATRAD51B, RAD51B, AT2G28560) wild-type |
Oryza sativa |
| (ATBRCA1, BRCA1, AT4G21070) |
was induced by zeocin in |
wild-type |
Arabidopsis thaliana |
| NAC103-GFP overexpressing (NAC103-OE) lines |
showed commonly and significantly enhanced transcription levels of |
(AT4G22960) |
Arabidopsis thaliana |
| E2FcRNAi plants |
show similar DNA damage as found in |
WT plants |
Arabidopsis thaliana |
| QDE-2-interacting small RNAs (qiRNAs) |
are derived from |
rDNA repeats |
Neurospora crassa |
| ATM-mediated DNA damage response |
is required to trigger |
death of CSCDs |
|
| (h2a.w.7, HTA7, AT5G27670) mutant expressing S135D mutant |
did not complement |
sensitivity of (h2a.w.7, HTA7, AT5G27670) to DNA damage |
Arabidopsis thaliana |
| improved accessibility to DNA damage response machinery within heterochromatin |
would explain |
delayed dynamics of DNA damage response (DDR) in heterochromatin |
Arabidopsis thaliana; mammals |
| Orysa;CDKB2;1 |
accumulates in response to |
DNA damage |
Oryza sativa |
| X-ray sensitivity of B2RNAi plants |
analyzed |
effect of CDKB2;1 knockdown on DNA damage response |
|
| induction of DSBs |
up-regulates |
CDK inhibitors |
Arabidopsis thaliana |
| chilling stress |
can cause |
DNA strand breaks |
|
| (ANAC008, SOG1, AT1G25580) |
is essential for |
efficient activation of DNA repair pathways |
|
| restriction enzymes with four-base recognition sequences |
induce greater than two-fold |
(ATBRCA1, BRCA1, AT4G21070) expression |
Arabidopsis thaliana |
| DNA repair |
is improved in |
(FAS1, FUGU2, NFB2, AT1G65470) m123-2 |
Arabidopsis thaliana |
| DNA repair gene (ATRAD51, RAD51, AT5G20850) |
was not increased in |
pp7l-1 and pp7l-3 mutant lines |
Arabidopsis thaliana |
| main-2 and pp7l-1 mutants |
were both able to transcriptionally respond to |
DNA damage treatment |
Arabidopsis thaliana |
| alt1-2 mutant |
demonstrated substantial reduction in root growth in presence of |
1 mM hydroxurea |
Arabidopsis thaliana |
| DNA DSBs in Arabidopsis endoreduplicating differentiating cells |
do not cause |
cell-cycle arrest at G 1 /S |
Arabidopsis thaliana |
| Adachi et al. (2011) |
demonstrated |
expression of Arath ;CDKB2;1 was suppressed in response to DSBs at both transcriptional and protein level |
Arabidopsis thaliana |
| DDR genes |
are involved in |
DNA repair |
|
| non-recombinogenic functions in DNA repair |
are suggested for |
some HR proteins |
|
| GH1-HMGA1 |
is identified as |
factor involved in DNA damage repair in Arabidopsis |
Arabidopsis thaliana |
| oxidative stress |
can cause |
DNA strand breaks |
|
| DNA damage detection |
leads to activation of |
ATAXIA-TELANGIECTASIA MUTATED (ATATM, ATM, ATM-1, PIG1, AT3G48190) |
|
| DNA damage response (DDR)-related genes |
would be expected to be increased if |
cell death was due to impaired genome stability |
Arabidopsis thaliana |
| typical symptoms of active DNA damage response |
includes |
increased expression of DNA repair genes |
|
| Cells deficient in any of the (ATRAD51, RAD51, AT5G20850) paralogs |
show |
similar phenotype of elevated frequencies of chromosomal instability |
|
| (ATRAD51B, RAD51B, AT2G28560) |
represents the main functionality of |
single-strand annealing (SSA) recombination repair |
Oryza sativa |
| SUPPRESSOR OF GAMMA RESPONSE 1 (ANAC008, SOG1, AT1G25580) |
is a key transcription factor that governs |
DNA damage response |
Arabidopsis thaliana |
| ATAXIA-TELANGIECTASIA MUTATED (ATATM, ATM, ATM-1, PIG1, AT3G48190) |
phosphorylates |
SUPPRESSOR OF GAMMA RESPONSE 1 (ANAC008, SOG1, AT1G25580) |
Arabidopsis thaliana |
| (ATBZIP60, BZIP60, AT1G42990) transcription level |
did not increase after |
zeocin treatment |
Arabidopsis thaliana |
| γ-H2AX |
potential function in promotion of |
HR activity in plants |
Arabidopsis thaliana |
| (AtTK1a, TK1, TK1a, AT3G07800) |
is upregulated by |
genotoxic agents such as mitomycin C and UV-C radiation |
Arabidopsis thaliana |
| (AtTK1a, TK1, TK1a, AT3G07800) and RNR subunit genes |
are |
direct targets of (ANAC008, SOG1, AT1G25580) |
Arabidopsis thaliana |
| diRNAs |
most likely function downstream of |
H2AX phosphorylation |
Arabidopsis thaliana |
| (AtMYB56, BRAVO, MYB56, AT5G17800) dynamics upon DNA damage |
suggest involvement in |
promoting quiescence |
|
| (SMR5, AT1G07500) |
is |
important for cell-cycle checkpoint activation following DNA damage |
Arabidopsis thaliana |
| CRL4 C3D E3 ubiquitin ligases |
targets for proteasomal degradation |
damaged DNA sensor (DDB2, AT5G58760) |
Arabidopsis thaliana |
| homologous recombination (HR) |
acts in |
repair of DNA breaks occurring after environmental stress |
|
| γ-ray induced (ATRAD51, RAD51, AT5G20850) focus formation |
is observed in |
(ATRAD51B, RAD51B, AT2G28560) (ATRAD51D, RAD51D, SSN1, AT1G07745) and (ATXRCC2, XRCC2, AT5G64520) mutant plants |
Arabidopsis thaliana |
| pyrimidine dimers |
inhibit |
transcription |
|
| ATAXIA-TELANGIECTASIA MUTATED (ATATM, ATM, ATM-1, PIG1, AT3G48190) and /RAD3-RELATED (ATATR, ATR, ATR-2, ATRAD3, AT5G40820) |
induce |
cell-cycle arrest |
|
| DNA damage response system (DDR) |
is crucial in addressing |
DNA double-strand breaks (DSBs) |
|
| (ANAC008, SOG1, AT1G25580) hyperphosphorylation |
is followed by |
activation of several (ANAC008, SOG1, AT1G25580) target genes |
Arabidopsis thaliana |
| (ANAC008, SOG1, AT1G25580) phosphorylation mutants |
demonstrate that |
hyperphosphorylation sites remain unchanged over time |
Arabidopsis thaliana |
| (AtPRD1, MEI1, PRD1, AT4G14180) |
is |
homolog of human HsTOPBP1 |
|
| meristematic cells |
exhibit increasing trend of |
(ATRAD51, RAD51, AT5G20850) mRNAs |
Arabidopsis thaliana |
| DNA damage |
increases |
transcriptional output per cell |
|
| DDR via HR |
is associated with |
S and G2 phases of the cell cycle |
|
| (ATRAD54, CHR25, RAD54, AT3G19210) foci |
emerge with high frequency in |
G1 and G2 cells |
Arabidopsis |
| SOS response |
is turned on by |
DNA damage |
Escherichia coli |
| ddrm2-2 (ATRAD51, RAD51, AT5G20850) double mutant |
is more sensitive to |
(ACPT, AtcPT3, CPT, cPT3, AT2G23410) (camptothecin) |
|
| cell cycle arrest |
is |
integral part of DNA damage response |
|
| stele cells |
show distinct RAD51 transcriptional response compared to |
other root cell types (Epidermis, Cortex, Endodermis) |
Arabidopsis thaliana |
| faster proliferation rates |
correlate with |
increased susceptibility to DNA damage |
|
| zeocin |
promotes arrest at |
G2/M checkpoint |
|
| (CYC1, CYCB1, CYCB1;1, AT4G37490) |
responds to |
DNA damage |
Arabidopsis thaliana |
| (ANAC008, SOG1, AT1G25580) (Suppressor of Gamma Response1) |
plays a central role in |
transcriptional regulation of DNA repair genes |
Arabidopsis thaliana |
| (PARP1, AT2G31320) |
contains |
BRCT domain |
|
| (ATRAD51, RAD51, AT5G20850) transcriptional response |
shows consistent variations among |
distinct cell types and developmental zones |
Arabidopsis thaliana |
| variations in abundance of cis-regulators |
may explain |
varying (ATRAD51, RAD51, AT5G20850) mRNA transcriptional output |
|
| ddrm2-2 (ATRAD51, RAD51, AT5G20850) double mutant |
is more sensitive to CPT than |
ddrm2-2 and (ATRAD51, RAD51, AT5G20850) single mutants |
Arabidopsis thaliana |
| cells in the root tips of X-ray-irradiated B2RNAi-7 mutant |
were |
swollen and disorganized |
|
| (ATATM, ATM, ATM-1, PIG1, AT3G48190) mutant |
is hypersensitive to |
double-strand break (DSB)-inducing reagents |
Arabidopsis thaliana |
| increasing zeocin concentrations |
increases |
number of DSBs per cell |
Arabidopsis thaliana |
| study results |
shed light on |
DNA damage response in plants |
|
| H2A.X phosphorylation in (FAS1, FUGU2, NFB2, AT1G65470) m123-2 |
was not affected in |
(FAS1, FUGU2, NFB2, AT1G65470) m123-2 |
Arabidopsis thaliana |
| cell cycle arrest |
ensures |
integrity of genetic material |
|
| G2/M reporter expression |
potentially corresponds to |
checkpoint arrest |
Arabidopsis thaliana |
| hydroxyurea (HU) treatment |
activates |
G2/M cell cycle arrest |
|
| study results |
uncover |
distinctions in transcriptional response of (ATRAD51, RAD51, AT5G20850) across cell types |
|
| DDRM2 expression induction by DSBs |
is |
SOG1-dependent manner |
Arabidopsis thaliana |
| DDRM2 |
is a target gene of |
(ANAC008, SOG1, AT1G25580) (Suppressor of Gamma Response1) |
Arabidopsis thaliana |
| BRAD1 |
contains |
BRCT domain |
|
| Arabidopsis thaliana |
lacks |
homolog of MDC1 |
Arabidopsis thaliana |