| KNOLLE |
is exclusively expressed in |
dividing cells throughout cytokinesis |
Arabidopsis thaliana |
| cell plate |
is active site of |
cell wall and membrane biosynthesis |
|
| (ATN, ATTAN, TAN1, AT3G05330) (AIR9, AT2G34680) double mutant misoriented phragmoplasts |
might be due to |
failure in phragmoplast guidance back to the division site |
Arabidopsis thaliana |
| Mob-1-like protein/phocein family |
is localized in |
cell division plane |
Medicago truncatula |
| phragmoplast MT in (ASG6, CRK2, AT1G70520) late anaphase and early telophase |
compacted without |
array formation |
Zea mays |
| PAN1 |
participate in |
cell plate development |
Zea mays |
| secretion defects in plants |
lead to |
cell division defects |
|
| phragmoplast angles of TAN1-∆II-YFP |
did not rescue as well as |
TAN1-YFP |
Arabidopsis thaliana |
| NPK1 |
is involved in the regulation of |
cytokinesis |
Nicotiana tabacum |
| mutation in the SNARE KNOLLE |
leads to |
enlarged embryo cells with multiple nuclei |
Arabidopsis thaliana |
| deletion of (AIR9, AT2G34680) in Trypanosoma brucei |
altered |
cleavage furrow and nuclear positioning |
Trypanosoma brucei |
| (ANP2, MAPKKK2, NP2, AT1G54960) (ANP3, AtANP3, MAPKKK12, NP3, AT3G06030) double mutant |
displays |
abnormal cytokinesis |
Arabidopsis thaliana |
| callose |
plays a role in |
cell plate maturation |
Arabidopsis thaliana |
| (AIR9, AT2G34680) protein |
localized to |
cell plate insertion site when the phragmoplast reached the mother cell cortex |
|
| PAN1 |
may function in |
attachment of cell plate to mother cell wall |
Zea mays |
| division site marker |
recruits |
division machinery |
|
| (ATN, ATTAN, TAN1, AT3G05330) and (AIR9, AT2G34680) |
are critical for |
phragmoplast guidance to the division site |
Arabidopsis thaliana |
| (ATN, ATTAN, TAN1, AT3G05330) -YFP (AIR9, AT2G34680) phragmoplasts |
were significantly more variable than |
(AIR9, AT2G34680) single mutant phragmoplast angles |
Arabidopsis thaliana |
| ANP subfamily of MAP3K (mitogen-activated protein kinase kinase kinase) |
have been shown to functionally interact with |
proteins thought to control stability and turnover of microtubules |
Arabidopsis thaliana |
| (ATFH8, FH8, FORMIN 8, AT1G70140) |
localizes to |
newly formed cell wall |
Arabidopsis thaliana |
| cell plate maturation |
is |
multifaceted process |
|
| ER tubules |
are associated with |
early phragmoplast |
|
| KNOLLE |
is required throughout |
cell plate formation |
Arabidopsis thaliana |
| pan1 mutants |
exhibited no defects in |
recruitment of cell plate components |
Zea mays |
| late anaphase |
is when vesicle delivery is guided by |
phragmoplast |
|
| microtubules at the center of the phragmoplast |
are disassembled |
microtubule disassembly |
|
| cell plate formation |
involves |
ongoing fusion and fission of vesicles |
|
| ES7 (endosidin 7) |
affords |
direct inhibition of cytokinesis |
Arabidopsis thaliana |
| pan1 mutant |
shows no difference in |
cell plate-associated F-actin |
Zea mays |
| caffeine treatment |
changed |
morphology of the phragmoplast |
Arabidopsis thaliana |
| caffeine |
disrupts |
cell plate formation |
Arabidopsis thaliana |
| (TPLATE, AT3G01780) |
functions in |
cell plate maturation |
|
| cell plate and parental plasma membrane fusion |
concludes |
separation of daughter cells |
|
| interface wall |
becomes |
apoplastic space |
Nicotiana tabacum |
| ES7 (endosidin 7) treatment |
resulted in normal |
phragmoplast formation |
Arabidopsis thaliana |
| phragmoplast |
is disassembled |
phragmoplast disassembly |
|
| callose deposition |
occurs during |
cell division |
|
| (ATMAP65-1, MAP65-1, AT5G55230) |
regulates |
stability and turnover of phragmoplast microtubules |
Arabidopsis thaliana |
| ConcA (concanamycin A) treatment |
resulted in normal |
phragmoplast formation |
Arabidopsis thaliana |
| actin |
participates in |
cytokinesis |
|
| Sec14p-like proteins |
coordinate |
vesicle trafficking to new cell wall of dividing cells |
Arabidopsis thaliana |
| extreme heat stress |
causes absence of |
typical cross-shaped cell wall between separated nuclei |
Arabidopsis thaliana |
| (ATEXO70A1, EXO70A1, AT5G03540) |
function is found in |
cell plate formation |
Arabidopsis thaliana |
| (ATFH8, FH8, FORMIN 8, AT1G70140) |
localized to |
new cell wall after cytokinesis |
Arabidopsis thaliana |
| actin microfilaments in the developing phragmoplast |
have |
widely spaced gap in the middle |
|
| membrane trafficking processes involved in cytokinesis |
show similarities in |
plants and animals |
|
| phragmoplast initial double ring structure |
organized from |
spindle mid-zone |
Nicotiana tabacum |
| microtubule-dependent microtubule polymerization |
is a mechanism underlying |
centrifugal expansion of the phragmoplast |
|
| membrane trafficking |
is central to |
cell plate formation |
|
| (AIR9, AT2G34680) |
accumulates at |
division site when phragmoplast reaches cell cortex |
Arabidopsis thaliana |
| callose |
is involved in |
cell plate formation |
|
| localization difference between plant kinesins and animal counterparts |
implies |
mechanistic differences in mitosis and cytokinesis between plants and animals |
|
| (POK2, AT3G19050) |
localizes to |
phragmoplast midzone |
|
| OsKCH1 signals during cytokinesis |
were repartitioned and subsequently mainly found surrounding |
the newly formed nuclei |
Nicotiana tabacum |
| TMBP200 depletion |
associated with striking defects in |
phragmoplast orientation |
Nicotiana tabacum |
| abnormal spores with disoriented or disorganized phragmoplasts |
proceed into |
cytokinesis |
Nicotiana tabacum |
| cell division |
results in |
(AGR, AGR1, ATPIN2, EIR1, MM31, PIN2, WAV6, AT5G57090) localization at both sides of cell plate |
Arabidopsis thaliana |
| Cytokinesis-related SNAREs |
traffic as |
preformed CIS-complexes |
|
| (POK2, AT3G19050) |
assumes dual localization at |
cortical division zone and phragmoplast midzone |
|
| chromosome segregation |
precedes |
central spindle and phragmoplast formation |
|
| phosphatidylinositol 4,5-bisphosphate |
regulates during |
cytokinesis |
|
| PPB |
recruits |
actomyosin system |
|
| (POK1, AT3G17360) and (POK2, AT3G19050) |
play redundant role in |
division plane orientation |
|
| Arabidopsis mutants of HINKEL/STUD1 kinesins and mitogen-activated protein KKK |
show |
obvious cytokinetic defects |
Arabidopsis thaliana |
| wild-type plants at early bicellular stage |
observed ∼9% of pollen with |
unexpanded cell plates |
|
| phragmoplast-driven cell division |
occurs in organisms that lack |
preprophase band |
mosses; algae |
| actin microfilaments in the developing phragmoplast |
form pattern similar to |
microtubules |
|
| CAMPs in Arabidopsis |
reminds of |
comparable activities of actomyosin at the cortical division zone in fission yeast |
Arabidopsis thaliana; Schizosaccharomyces pombe |
| actin microfilaments |
are detected at |
cell cortex |
|
| myosin (ATXIK, XI-17, XI-K, XIK, AT5G20490) |
assumes dual localization at |
cortical division zone and phragmoplast midzone |
|
| overexpression of GhADF7 gene |
results in |
defective cytokinesis in fission yeast |
Schizosaccharomyces pombe |
| TMBP200 depletion |
associated with striking defects in |
phragmoplast position |
Nicotiana tabacum |
| cell cycle-dependent localizations of actin microfilaments |
do not coincide with |
execution of cytokinesis per se in somatic cells |
|
| depletion of both (ATNACK1, HIK, NACK1, AT1G18370) and (ATNACK2, NACK2, TES, AT3G43210) products |
disrupts |
cytokinesis |
|
| continuous microtubule assembly at the phragmoplast periphery |
results in |
centrifugal expansion of the phragmoplast |
|
| phragmoplast |
constructs |
cell plate |
|
| phragmoplast |
is |
one of two temporally separated cytokinetic apparatuses |
|
| phragmoplast |
continues to expand outward until |
cell plate connects to parental cell wall |
|
| precise coordination of microtubule organization with vesicle trafficking, membrane remodeling, and deposition of oligosaccharides |
plays a critical role for |
cell plate production |
|
| (KINESIN-12A, PAKRP1, AT4G14150) and (KINESIN-12B, PAKRP1L, AT3G23670) |
play redundant role in organizing |
anti-parallel phragmoplast array at PM I |
Arabidopsis thaliana |
| TMBP200 depletion |
associated with striking defects in |
phragmoplast structure |
Nicotiana tabacum |
| densely packed MTs in the midzone of the telophase spindle |
initiate |
phragmoplast |
Nicotiana tabacum |
| (ATMYA1, MYA1, XI-1, AT1G17580) and (POK1, AT3G17360) |
may be associated with each other physically so that |
their functions are coupled seamlessly in cytokinesis |
|
| stud/ (ATNACK2, NACK2, TES, AT3G43210) mutant |
exhibits cytokinesis failure in |
male meiosis |
|
| NACK–PQR MAPKKK pathway |
functions during |
cell plate expansion |
Arabidopsis thaliana |
| altered balance of actin depolymerization and polymerization |
led to |
multinucleate formation |
Schizosaccharomyces pombe |
| NQK1/NtMEK1 |
is required for |
cell cytokinesis |
Nicotiana tabacum |
| positioning of the plus ends of cross-linked microtubules at or near the division site |
is a mechanism underlying |
centrifugal expansion of the phragmoplast |
|
| OsKCH1 subsequent to the division |
reassembled around |
the newly formed daughter nuclei |
Nicotiana tabacum |
| STUD/ (ATNACK2, NACK2, TES, AT3G43210) |
encodes |
kinesin |
|
| complex phragmoplast forms |
account for |
striking profiles of internal cell walls in TMBP200 RNAi lines |
Nicotiana tabacum |
| (POK1, AT3G17360) |
resides in |
cortical division zone |
|
| heterologous expression of GhADF7 gene in yeast cells |
led to formation of |
multinucleate cells |
Schizosaccharomyces pombe |
| centrifugal expansion of the phragmoplast |
takes place concomitantly with |
assembly of the cell plate |
|
| phragmoplasts in TMBP200 RNAi plants |
are significantly shorter in |
microspores from TMBP200 RNAi plants |
Nicotiana tabacum |
| parthenolide-treated cells |
show |
wavy and partially non-contiguous cell plate |
Arabidopsis thaliana |
| (ATMAP65-3, MAP65-3, PLE, AT5G51600) |
locates to |
midline or clear zone |
Arabidopsis thaliana |
| (FU, TIO, AT1G50240) |
only appears tightly localized to the midline after |
phragmoplast assembly |
Arabidopsis thaliana |
| plant nuclear envelope reassembly |
appears complete concomitant with |
early cell-plate formation |
Arabidopsis thaliana |
| phragmoplast rotation |
results in cell plate attaching at |
former preprophase band (PPB) site |
|
| cell plate expansion |
occurs during |
cytokinesis |
Arabidopsis thaliana |
| KNOLLE (KN) signal |
is enlarged by 42% in |
runkel (EMB3013, RUK, AT5G18700) mutant cells |
Arabidopsis thaliana |
| actomyosin system |
is required for |
centrifugally expanding phragmoplast to recognize cortical division zone |
|
| myosin XI motors |
form macromolecular assemblies with |
Kinesin-12 motors and other microtubule-associated proteins |
|
| CAMP analogous assemblies |
are captured by |
CAMPs stationed at cortical division zone |
|
| different modes of cytokinesis |
are executed by |
common signaling pathway |
Arabidopsis thaliana |
| completion of cell plate expansion at pollen mitosis I (PMI) |
requires either |
(ATNACK1, HIK, NACK1, AT1G18370) or (ATNACK2, NACK2, TES, AT3G43210) |
|
| gem2 mutant |
resulted in |
repositioning of cell plate and partial or complete failure of cytokinesis |
Arabidopsis thaliana |
| experimentally induced doubling of the microtubular pre-prophase band |
affects |
cell plate formation |
|
| unexpanded cell plates in wild-type at early bicellular stage |
most likely representing |
earlier stages of cytokinesis |
|
| several kinesins |
are required for |
phragmoplast growth |
Arabidopsis thaliana |
| Eleftheriou et al. (2005) |
observed |
aberrant phragmoplasts in root cells of (GEM1, MOR1, AT2G35630) mutants |
Arabidopsis thaliana |
| exocyst |
is involved in regulation of |
cytokinesis |
|
| (POK1, AT3G17360) motility |
may be required for |
CAMP assembly and function |
|
| quantitative analysis of PPB and phragmoplast orientation |
suggested |
phragmoplast guidance is defective in (ATVPS52, POK, TTD8, VPS52, AT1G71270) mutants |
Arabidopsis thaliana |
| single continuous phragmoplasts but with abnormal position, profiles, or orientation |
are likely to be initiated from |
misoriented bipolar spindles |
Nicotiana tabacum |
| plant cytokinesis |
is brought about by |
microtubule-based phragmoplast |
|
| CAMPs |
receive |
microtubules emanating from edge of expanding phragmoplast |
|
| reorganization of OsKCH1 during cytokinesis |
was observed during |
cytokinesis |
Nicotiana tabacum |
| phragmoplast edge |
becomes docked at |
PPB-demarcated site |
|
| clear zones in phragmoplasts of (ATMAP65-3, MAP65-3, PLE, AT5G51600) mutants |
are |
wider |
Arabidopsis thaliana |
| drift of two halves of phragmoplast |
is even and does not |
disrupt shape of phragmoplast |
Arabidopsis thaliana |
| incompletely penetrant pollen cytokinesis phenotypes in tio-1 and tio-2 |
further suggest |
C-terminally truncated (FU, TIO, AT1G50240) retains partial function |
Arabidopsis thaliana |
| transverse sections through arrested shoot and root meristems |
revealed |
multinucleate cells with incomplete cell walls |
Arabidopsis thaliana |
| KNOLLE |
disappears from |
completed cell plate |
|
| KNOLLE (ATSYP111, KN, SYP111, AT1G08560) |
is involved in |
cytokinesis |
Arabidopsis thaliana |
| cytokinesis |
was completed |
wortmannin-treated cells |
Arabidopsis thaliana |
| targeting to the plane of cell division |
is essential for |
KNOLLE and other proteins that are necessary for cytokinesis |
Arabidopsis thaliana |
| phragmoplast |
is initially oriented obliquely but rotates as |
cytokinesis proceeds |
|
| GFP-EDE1 |
decorated |
phragmoplast as it expanded toward the cell cortex |
Arabidopsis thaliana |
| re-polymerisation of microtubules at the leading edge |
enables |
phragmoplast expansion |
|
| ProMSP1-TFΔC-3MYC |
rescued to lesser extent (32%) than |
ProMSP1-TF-3MYC (24%) |
|
| (ABCB23, ATATM1, ATM1, AT4G28630) |
localizes to |
developing cell plate |
Arabidopsis thaliana |
| ANX11 |
is recruited to |
midbody in late telophase |
|
| inactivation of microtubule-bundling by tobacco (ATMAP65-1, MAP65-1, AT5G55230) |
allows |
phragmoplast expansion |
Nicotiana tabacum |
| components of signalling pathway |
locate to |
phragmoplast midline |
Nicotiana tabacum |
| (FU, TIO, AT1G50240) microspores |
initiate but fail to complete |
cell plate |
|
| (ATNPSN11, NPSN11, NSPN11, AT2G35190) |
is immuno-fluorescently localized on |
cell plate |
Arabidopsis thaliana |
| phragmoplast |
directs |
cell plate formation through delivery of Golgi-derived vesicles |
|
| plant cells |
have relative cell positions permanently established when |
daughter cells are formed at cytokinesis |
|
| protein T-PLATE |
is implicated in |
cell-plate attachment at the cortical division site and/or cell-plate maturation |
|
| late phragmoplasts in tan-csh mutant root tips |
were tilted |
≥ 15° from transverse or longitudinal axis of mother cell |
Arabidopsis thaliana |
| wider clear zones and phragmoplasts in (ATMAP65-3, MAP65-3, PLE, AT5G51600) mutants |
emphasizes |
importance of (ATMAP65-3, MAP65-3, PLE, AT5G51600) for integrity of phragmoplast in cytokinesis |
Arabidopsis thaliana |
| (FU, TIO, AT1G50240) (TWO-IN-ONE) |
remains associated with |
expanding phragmoplast ring |
Arabidopsis thaliana |
| (ATMAP65-3, MAP65-3, PLE, AT5G51600) |
is essential for |
completing cell division in roots |
Arabidopsis thaliana |
| developing cell plate |
can be inserted into |
spatially defined position |
|
| plant cells |
employ physically associated |
myosin and kinesin motors |
|
| (GEM1, MOR1, AT2G35630) |
has role in |
phragmoplast |
Arabidopsis thaliana |
| intact microtubules and actin microfilaments |
coordinate actions of |
keeping preprophase band (PPB)-marked cortical division zone competent in receiving expanding edge of phragmoplast |
|
| similar microtubule-associated complex and mitogen-activated kinase signalling to the phragmoplast |
is likely present in |
Arabidopsis |
Arabidopsis thaliana |
| microtubule-associated protein (AIR9, AT2G34680) |
is implicated in |
cell-plate attachment at the cortical division site and/or cell-plate maturation |
|
| (EMB3013, RUK, AT5G18700) protein presence during cytokinesis |
is necessary to |
execute (EMB3013, RUK, AT5G18700) function |
Arabidopsis thaliana |
| DRP1A-mRFP1 protein |
largely disappears after |
cell plate fusion with the plasma membrane |
Arabidopsis thaliana |
| discrete subcellular mechanical hallmarks |
guide cells to avoid |
four-way junction formation during cytokinesis |
|
| cytokinesis in sporophytic and gametophytic cells in plants |
exhibits differing modes to fulfill |
cell type-specific requirements |
|
| NACK-PQR pathway |
promotes |
destabilization of phragmoplast microtubules through MAP65 activity |
Arabidopsis thaliana |
| Arabidopsis (FU, TIO, AT1G50240) kinase |
has |
essential role in the phragmoplast during cell plate expansion |
Arabidopsis thaliana |
| cell plate |
is abnormally shaped in |
runkel (EMB3013, RUK, AT5G18700) mutant cells |
Arabidopsis thaliana |
| mutants impaired in PPB formation |
hardly display |
defects in cell division |
|
| (FU, TIO, AT1G50240) mutants |
exhibit |
phragmoplast persists as short structure |
Arabidopsis thaliana |
| (FU, TIO, AT1G50240) constructs with mutations in kinase active site |
fail to rescue |
tio-3 mutant |
Arabidopsis thaliana |
| sterols |
are crucial for |
cytokinesis |
|
| sterols |
accumulate in |
septum during cytokinesis of Schizosaccharomyces pombe |
Schizosaccharomyces pombe |
| lovastatin (lov)-treated roots |
display significantly lower GP values at |
cell plate |
Arabidopsis thaliana |
| (ADL1, ADL1A, AG68, DL1, DRP1A, RSW9, AT5G42080) localization |
may be regulated by |
membrane sterol composition |
|
| cytokinesis in plant cells |
is directed by |
phragmoplast |
|
| (ATNPSN11, NPSN11, NSPN11, AT2G35190) |
interacts with |
KNOLLE |
Arabidopsis thaliana |
| several kinesin-related proteins |
are associated with |
phragmoplast midline |
|
| hydroxyproline-rich glycoprotein (ATEXT3, EXT3, RSH, AT1G21310) |
is localized at |
cortical division site or adjacent cell wall |
|
| mutant (EMB3013, RUK, AT5G18700) proteins (K33W and D121A) |
were properly localized on |
phragmoplast microtubules during mitosis |
Arabidopsis thaliana |
| Transgenic Arabidopsis thaliana expressing bgl23-D cDNA with FAMA promoter |
exhibits higher frequency of |
bagel-shaped stomata with severe cytokinesis defects |
Arabidopsis thaliana |
| isolation of first MAP65 mutants with distinct cytokinesis defect |
demonstrates |
(ATMAP65-3, MAP65-3, PLE, AT5G51600) mutants have cytokinesis defect |
Arabidopsis thaliana |
| failure of cell plate expansion in tio-3 null mutants |
demonstrates |
requirement for (FU, TIO, AT1G50240) in the phragmoplast during cell plate expansion |
Arabidopsis thaliana |
| mechanism of narrowing of the TANGLED (ATN, ATTAN, TAN1, AT3G05330) ring at the onset of cytokinesis |
is most likely quite different from |
the mechanism of coalescence of the Mid1p ring |
Arabidopsis thaliana; Schizosaccharomyces pombe |
| enlarged runkel (EMB3013, RUK, AT5G18700) mutant cells during cytokinesis |
display |
KN-labeled cell plates |
Arabidopsis thaliana |
| (EMB3013, RUK, AT5G18700) |
is |
novel microtubule-associated protein required for cell plate expansion |
Arabidopsis thaliana |
| post-mitotic phragmoplast formation |
defines |
orientation of new cross wall |
|
| microtubules at boundary of cell plate |
remain and are continually |
turning over |
Arabidopsis thaliana |
| KNOLLE |
failed to overlap with |
phragmoplast microtubules at the division plane |
Arabidopsis thaliana |
| proportion of phragmoplasts not properly guided to former PPB sites in tan-csh mutant root tips |
is |
significant (P < 0.01) |
Arabidopsis thaliana |
| RUNKEL (EMB3013, RUK, AT5G18700) |
encodes |
RUNKEL (EMB3013, RUK, AT5G18700) protein |
Arabidopsis thaliana |
| cell plate |
is more electron dense in |
runkel (EMB3013, RUK, AT5G18700) mutant cells |
Arabidopsis thaliana |
| PIN accumulation on the cell plate |
increases only after |
the new septum is formed |
Arabidopsis thaliana |
| ConcA-treated root tips |
showed |
binucleate cells |
Arabidopsis thaliana |
| disintegration of AtTAN::YFP puncta |
occurs |
10–20 min after phragmoplast disassembly |
Arabidopsis thaliana |
| TANGLED (ATN, ATTAN, TAN1, AT3G05330) at the division site |
interacts with |
the expanding phragmoplast during cytokinesis |
Arabidopsis thaliana |
| (ATNPSN11, NPSN11, NSPN11, AT2G35190) |
involved in |
cytokinesis |
Arabidopsis thaliana |
| distorted phragmoplast in (ATMAP65-3, MAP65-3, PLE, AT5G51600) mutants |
could arise from |
absence of (ATMAP65-3, MAP65-3, PLE, AT5G51600) destabilizing region of overlap at midline |
Arabidopsis thaliana |
| PHRAGMOPLAST ORIENTING KINESIN 1 (POK1, AT3G17360) and PHRAGMOPLAST ORIENTING KINESIN 2 (POK2, AT3G19050) |
participate in |
spatial control of cytokinesis |
Arabidopsis thaliana |
| endocytosis |
is proposed to contribute substantially to |
cell-plate formation |
Arabidopsis thaliana |
| phragmoplasts in (ATMAP65-3, MAP65-3, PLE, AT5G51600) mutants |
revealed |
significant size differences |
Arabidopsis thaliana |
| even drift without disruption of phragmoplast shape |
indicates |
two halves must be held in place by another force |
Arabidopsis thaliana |
| (FU, TIO, AT1G50240) localization to the midline of the phragmoplast |
is in the region where |
phragmoplast Mts overlap |
Arabidopsis thaliana |
| (POK1, AT3G17360) (POK2, AT3G19050) double mutants |
phenotype strongly resembles |
maize (ATN, ATTAN, TAN1, AT3G05330) mutants phenotype |
Arabidopsis thaliana; Zea mays |
| cytokinetic phragmoplast |
expands laterally to attach |
new cell wall |
Arabidopsis thaliana |
| microtubule-associated protein (AIR9, AT2G34680) |
reappears at |
cortical division site |
|
| hydroxyproline-rich glycoprotein (ATEXT3, EXT3, RSH, AT1G21310) |
is implicated in |
cell-plate attachment at the cortical division site and/or cell-plate maturation |
|
| AtTAN::YFP rings |
persisted through |
completion of cell-plate insertion |
Arabidopsis thaliana |
| (EMB3013, RUK, AT5G18700) protein with N-terminal 6xHA epitope |
localized to |
phragmoplast |
Arabidopsis thaliana |
| highest FRET signal |
is detected on |
growing ends of the cell plate |
Nicotiana tabacum |
| absence of obvious PPB defects in mutants |
suggests presence of |
alternative mechanism for cell division site positioning |
|
| complete karyokinesis and incomplete cytokinesis in (ATMAP65-3, MAP65-3, PLE, AT5G51600) mutants |
together with phragmoplast measurements conclude |
(ATMAP65-3, MAP65-3, PLE, AT5G51600) is essential for phragmoplast function |
Arabidopsis thaliana |
| (FU, TIO, AT1G50240) (TWO-IN-ONE) |
has essential role in |
conventional modes of cytokinesis in plant meristems |
Arabidopsis thaliana |
| KNOLLE |
returns from |
cell plate |
Arabidopsis thaliana |
| excessive tethering of microtubules with actin filaments |
is expected to impair |
deposition of endosomal belt subtending microtubular pre-prophase band |
|
| (FU, TIO, AT1G50240) (TWO-IN-ONE) |
is tightly localized to |
midline of nascent phragmoplast |
Arabidopsis thaliana |
| RUNKEL (EMB3013, RUK, AT5G18700) protein |
colocalized with |
phragmoplast |
Arabidopsis thaliana |
| ede1-1 mutant |
compromises |
γ-tubulin localization on phragmoplast microtubule arrays |
Arabidopsis thaliana |
| establishment of cell plate in (FU, TIO, AT1G50240) mutants |
appears to be |
normal at early stages |
Arabidopsis thaliana |
| cytokinesis defects |
caused by |
abnormal phragmoplast organization |
Arabidopsis thaliana |
| RUNKEL (EMB3013, RUK, AT5G18700) protein |
colocalized with |
spindle |
Arabidopsis thaliana |
| (FU, TIO, AT1G50240) mutants |
leads to formation of |
callosic cell-plate fragments or stubs |
|
| plant cells |
have adopted |
cell type-specific modes of division |
|
| actin-filament bundles |
serve as tracks for |
transport of materials during cytokinesis |
|
| (EDE1, EMB3116, QWRF5, AT2G44190) (Endosperm DEfective 1) |
is associated with |
phragmoplast microtubule arrays |
Arabidopsis thaliana |
| axial width of phragmoplast MT array in ede1-1 mutant |
became wider |
compared with control cells during expansion of the array |
Arabidopsis thaliana |
| parthenolide |
causes |
disorientation of cross walls |
plants |
| plant cells |
generally divide centrifugally by |
building a cell plate |
|
| membrane vesicles |
fuse to form |
cell plate |
Arabidopsis thaliana |
| endocytic trafficking |
is not |
necessary in cytokinesis |
Arabidopsis thaliana |
| (ABCB23, ATATM1, ATM1, AT4G28630) localization in plasmodesmata |
suggests possible roles in |
cell-plate maturation |
|
| (ATMAP65-3, MAP65-3, PLE, AT5G51600) mutants |
have |
incomplete cytokinesis |
Arabidopsis thaliana |
| Golgi-derived vesicles |
fuse with one another in |
plane of cell division |
|
| punctate AtTAN::YFP ring |
was clearly visible as |
bright spots around entire cell equator |
Arabidopsis thaliana |
| low frequency of aberrantly oriented divisions in Arabidopsis tan mutant roots |
may indicate |
existence of TAN-independent mechanism acting together with TAN to guide phragmoplast expansion |
Arabidopsis thaliana |
| function of the TANGLED (ATN, ATTAN, TAN1, AT3G05330) ring |
is most likely quite different from |
the function of the Mid1p ring |
Arabidopsis thaliana; Schizosaccharomyces pombe |
| phragmoplast |
guides deposition of |
cell plate |
|
| exocyst-dependent PIN repolarization on plasma membrane domains after cytokinesis |
is possible |
exocyst involvement in cell plate maturation |
Arabidopsis thaliana |
| 1-NOA, 2-NOA, or CHPAA treatment at 20 μM for 24 h |
resulted in disruption of |
cell plate formation |
Nicotiana tabacum |
| pre-prophase band |
has guiding effect on |
spatial organization of phragmoplast and cell plate |
|
| (ATMAP65-3, MAP65-3, PLE, AT5G51600) |
is essential for |
maintaining integrity of overlapped microtubules in phragmoplast |
Arabidopsis thaliana |
| improper cell plate anchorage |
led to |
apolar cell division |
Nicotiana tabacum |
| (ATMAP65-3, MAP65-3, PLE, AT5G51600) mutants |
can form but is distorted |
cytokinetic phragmoplast |
Arabidopsis thaliana |
| (ATMAP65-3, MAP65-3, PLE, AT5G51600) mutants |
are defective in |
cytokinesis |
Arabidopsis thaliana |
| (FU, TIO, AT1G50240) (TWO-IN-ONE) |
has key role in |
cellularization during female gametogenesis |
Arabidopsis thaliana |
| new cell wall formation |
partitions |
cytoplasm of dividing cell |
|
| cell plate |
has |
carbohydrate composition |
|
| plant cell plate |
is |
high lipid-order membrane domain |
|
| sterol composition and membrane order |
affects |
cell-plate localization of (ADL1, ADL1A, AG68, DL1, DRP1A, RSW9, AT5G42080) |
Arabidopsis thaliana |
| high lipid-order membrane domains |
function as platforms for |
execution of cytokinesis |
|
| late cytokinesis |
coincides with onset of |
clathrin-coated vesicle (CCV) formation at the cell plate |
Arabidopsis thaliana |
| cell plate |
is constructed by |
new cell wall |
plant cells |
| (POK1, AT3G17360) (POK2, AT3G19050) double mutants |
characterized by misplaced |
cell walls |
Arabidopsis thaliana |
| traffic from the TGN to the plane of cell division |
is essential for |
cytokinesis |
Arabidopsis thaliana |
| secretory and endocytic trafficking pathways |
are both deflected to |
plane of cell division |
Arabidopsis thaliana |
| disintegration of AtTAN::YFP puncta |
followed |
disassembly of phragmoplast from corresponding position at cell periphery |
Arabidopsis thaliana |
| (EMB3013, RUK, AT5G18700) |
might be involved in |
microtubule dynamics during phragmoplast expansion |
Arabidopsis thaliana |
| DRP1 function in cell plate formation |
is most likely by |
tubulation and constriction of the fusion membranes and vesicles |
Arabidopsis thaliana |
| cell plates |
are usually formed between |
dividing nuclei located in the cell center |
|
| disorganized anti-parallel microtubules |
fail to establish |
functional phragmoplast midzone |
Arabidopsis thaliana |
| microtubule-binding domain |
is essential for |
RUNKEL (EMB3013, RUK, AT5G18700) function |
Arabidopsis thaliana |
| incomplete cytokinesis |
leads to |
binucleate cells |
Arabidopsis thaliana |
| short phragmoplasts in +/tio-3 microspores |
are located |
off-centre outside the inter-nuclear zone |
Arabidopsis thaliana |
| (ATMAP65-3, MAP65-3, PLE, AT5G51600) |
selectively cross-links |
interdigitating microtubules in midzone |
Arabidopsis thaliana |
| (ATMAP65-3, MAP65-3, PLE, AT5G51600) cross-linking of interdigitating phragmoplast microtubules |
subsequently guides |
Kinesin-12 and (FU, TIO, AT1G50240) to vicinity |
Arabidopsis thaliana |
| lack of Kinesin-12 members in presence of (ATMAP65-3, MAP65-3, PLE, AT5G51600) |
causes |
anti-parallel microtubules to overlap to form continuous bundles |
Arabidopsis thaliana |
| dynamic turnover |
is crucial for |
sustained constriction during cytokinesis |
|
| flufenacet treatment |
causes |
cytokinetic defects |
Arabidopsis thaliana |
| phragmoplast |
arises initially from |
microtubules of the spindle midzone |
|
| plant cells |
complete |
cytokinesis |
Arabidopsis thaliana |
| gametophytic failure of microspore cytokinesis |
results in formation of |
short, incomplete cell plates |
Arabidopsis thaliana |
| TIO-Kinesin-12 molecular module |
is essential for |
dynamic expansion of phragmoplast |
Arabidopsis thaliana |
| cytoskeleton |
plays an important role in |
cell division |
|
| plant-specific KCH kinesins |
are required for |
phragmoplast formation |
|
| total width of ple-1 mutant phragmoplast |
is |
5.64 ± 1.64 μm |
Arabidopsis thaliana |
| C-terminal 379 amino acids of (FU, TIO, AT1G50240) including the ARM repeat domain |
are not essential for |
(FU, TIO, AT1G50240) function |
Arabidopsis thaliana |
| many plasma membrane proteins, including PIN proteins |
localize to |
forming cell plate |
Arabidopsis thaliana |
| ede1-1 mutant |
undergoes cytokinesis following formation of |
elongated bipolar phragmoplast microtubule arrays |
Arabidopsis thaliana |
| turnover of microtubules |
enables |
phragmoplast expansion |
|
| short phragmoplasts in wt microspores |
are located |
centrally between the two daughter nuclei (98%) |
|
| enlarged KNOLLE (KN) signal |
might reflect |
wavy shape of cell plate |
Arabidopsis thaliana |
| plant cells |
build |
cell plate |
|
| (FU, TIO, AT1G50240) |
is required for |
cell-plate expansion during cytokinesis |
Arabidopsis thaliana |
| secretion machinery |
is directed to |
cell plate |
plant cells |
| Mid1p |
functions to recruit |
several other proteins that bring about the assembly and contraction of an F-actin-based cytokinetic ring |
Schizosaccharomyces pombe |
| (EMB3013, RUK, AT5G18700) |
unlike MOR1 also accumulated at |
midzone of early phragmoplast |
Arabidopsis thaliana |
| (EMB3013, RUK, AT5G18700) |
localized to |
expanding cell plate during late cytokinesis |
Arabidopsis thaliana |
| drp1 mutants |
display defects in |
cell plate biogenesis during cytokinesis |
Arabidopsis thaliana |
| (FU, TIO, AT1G50240) and Kinesin-12 signalling module |
is required to support |
phragmoplast expansion and cell-plate growth |
Arabidopsis thaliana |
| Kinesin-12 members |
function to |
organize and maintain anti-parallel phragmoplast microtubule array |
Arabidopsis thaliana |
| phragmoplast |
facilitates formation of |
cell plate via fusion of Golgi-derived vesicles |
|
| membrane vesicles |
are delivered to |
cell-division plane |
Arabidopsis thaliana |
| broad AtTAN::YFP rings |
sharpened further during |
phragmoplast expansion to cell periphery |
Arabidopsis thaliana |
| cytokinesis defects of runkel (EMB3013, RUK, AT5G18700) mutants |
suggest that role of RUK in cytokinesis might be related to |
interacting MAP kinase kinase kinase NPK1 |
Arabidopsis thaliana |
| (EMB3013, RUK, AT5G18700) localization pattern |
suggests |
mechanistically different contribution of (EMB3013, RUK, AT5G18700) to microtubule destabilization |
Arabidopsis thaliana |
| MTs in phragmoplast array |
soon had shortened so that |
axial width became reduced |
Arabidopsis thaliana |
| tio-3 mutant microspores |
establish |
phragmoplast |
|
| DRP1 family members of Arabidopsis thaliana |
are enriched at |
cell plate |
Arabidopsis thaliana |
| KNOLLE |
is ectopically found at |
lateral plasma membranes |
Arabidopsis thaliana |
| (BAF60, CHC1, SWP73B, AT5G14170) mutant |
does not affect |
KNOLLE cell-plate localization |
Arabidopsis thaliana |
| fluorescently tagged (CESA6, E112, IXR2, PRC1, AT5G64740) |
is detected at |
cell plate |
Arabidopsis thaliana |
| two mirrored halves of spindle midzone MTs |
marked |
birth of the phragmoplast MT array |
Arabidopsis thaliana |
| phragmoplast array |
expanded in |
horizontal direction toward the parental cell membrane |
Arabidopsis thaliana |
| cytokinesis defects |
caused by |
arrested cell plate expansion |
Arabidopsis thaliana |
| cell-wall stubs |
were often observed in addition, indicative of |
cytokinetic defects due to incomplete cell-plate formation |
|
| CDKB2;1–GFP fusion protein |
then localized to |
phragmoplast |
|
| function of (FU, TIO, AT1G50240) in planta |
depends largely on |
C-terminal domain consisting of four ARM/HEAT repeats and two highly conserved sequence motifs |
Arabidopsis thaliana |
| (FU, TIO, AT1G50240) |
is not located to |
other cortical or mitotic microtubule arrays |
Arabidopsis thaliana |
| lateral expansion of phragmoplast |
is |
key step in cell plate formation |
Oryza sativa |
| phragmoplast microtubule organization |
occurs during |
cell plate expansion |
Arabidopsis thaliana |
| (EMB3013, RUK, AT5G18700) protein |
has to be present during |
cytokinesis |
Arabidopsis thaliana |
| PIN polarization |
is not observed before |
completion of the cell plate |
Arabidopsis thaliana |
| KNOLLE and ARA7-GFP signals |
increasingly overlapped from |
cytokinesis on |
Arabidopsis thaliana |
| WIPs |
colocalize with RanGAP1 at |
cell plate |
Arabidopsis thaliana |
| runkel (EMB3013, RUK, AT5G18700) mutant phenotype |
appears to be caused by |
loss of (EMB3013, RUK, AT5G18700) protein |
Arabidopsis thaliana |
| PIN and DRP1 proteins |
form transient complexes during |
cell plate formation |
Arabidopsis thaliana |
| phragmoplast misorientation |
led to |
abnormal cell plate alignment |
Marchantia polymorpha; Arabidopsis thaliana |
| strong reduction or inhibition of endocytosis |
does not prevent |
cytokinesis |
Arabidopsis thaliana |
| tio-3 mutant microspores |
fail to expand phragmoplast and do not form |
typical ring structure |
|
| (FU, TIO, AT1G50240) |
functions specifically during |
phragmoplast expansion |
Arabidopsis thaliana |
| cell plate |
fuses with |
mother cell wall |
|
| (ATFH5, Fh5, AT5G54650) |
has been shown to localize in |
maturing cell plates |
Arabidopsis thaliana |
| N-terminus of AtFH8:GFP |
localizes to |
newly formed cell wall of mitotic cells |
Arabidopsis thaliana |
| (ATFH8, FH8, FORMIN 8, AT1G70140) |
localizes to |
newly formed cell wall |
Arabidopsis thaliana |
| exocyst |
appears to be required at |
terminal phase of cytokinesis at midbody |
Homo sapiens |
| anterograde traffic from the TGN |
forms |
cell plate |
Arabidopsis thaliana |
| changes causing KNOLLE protein degradation |
occurs at |
end of cytokinesis |
Arabidopsis thaliana |
| actin microfilaments (F-actin) |
plays a role in guiding |
expanding phragmoplast to precisely recognize the cell plate fusion site |
|
| (ATRANGAP1, RANGAP1, AT3G63130) |
is detected at |
phragmoplast midzone |
Arabidopsis thaliana |
| (PK3AT, WAG1, AT1G53700) AGC kinase co-localization with (AGR, AGR1, ATPIN2, EIR1, MM31, PIN2, WAV6, AT5G57090) |
possibly reflects |
ongoing phosphorylation of (AGR, AGR1, ATPIN2, EIR1, MM31, PIN2, WAV6, AT5G57090) in cytokinetic cells |
|
| KNOLLE syntaxin |
is localized to |
cell plate |
|
| plant cell plate |
represents |
high lipid-order membrane domain |
|
| reduction of sterol concentration by lov treatment |
reduced the amount of |
(ADL1, ADL1A, AG68, DL1, DRP1A, RSW9, AT5G42080) at the cell plate |
|
| critical sterol concentration |
is required for |
high membrane order |
|
| vacuoles |
form sausage-like tubular compartments during |
early telophase cytokinesis |
Arabidopsis thaliana |
| microtubules |
are involved in |
cytokinetic vesicle trafficking |
|
| perturbation of root mechanical homeostasis |
results in |
four-way junction formation |
|
| last 23 aa of (FU, TIO, AT1G50240) C terminus |
may be substituted, in part, by |
unrelated sequence |
|
| cell plate |
is initiated via |
vesicle fusion at phragmoplast equator |
|
| proteins regulating vesicle targeting, fusion, and fission |
are localized to |
cell plate membranes |
|
| enzymes directing cell wall and membrane biosynthesis |
are recruited to |
cell plate |
|
| ANP subfamily of MAP3K (mitogen-activated protein kinase kinase kinase) |
may localize to |
phragmoplast |
Arabidopsis thaliana |
| endosidin 7 (ES7) |
arrests |
late cytokinesis |
Arabidopsis thaliana |
| (ATN, ATTAN, TAN1, AT3G05330) -∆I-YFP (AIR9, AT2G34680) plants |
had only 19.2% of phragmoplast angles within 80° to 100° |
normal phragmoplast orientation |
Arabidopsis thaliana |
| (ANQ1, ATMKK6, MKK6, SUMM4, AT5G56580) |
is primarily known to be involved during |
cytokinesis |
Arabidopsis thaliana |
| MTs in WT late anaphase and early telophase |
accumulated in |
middle of two daughter nuclei to initiate formation of phragmoplast MT array |
Zea mays |
| (KEU, SEC11, AT1G12360) interaction |
might mediate |
Hc-L localization to the cell division plane |
Arabidopsis thaliana |
| KN-KEU interaction at the division plane |
is |
pivotal regulatory step for membrane fusion during cytokinesis |
Arabidopsis thaliana |
| (AtTRS120, TRAPPC9, TRS120, VAN4, AT5G11040) peak fluorescence |
was seen during |
cell plate formation |
|
| KNOLLE |
is |
cytokinesis-specific Qa-SNARE |
Arabidopsis thaliana |
| Klebsormidium |
displays |
centripetal furrowing |
Klebsormidium flaccidum |
| cytokinesis-specific SNARE complexes |
exist in |
Arabidopsis |
Arabidopsis thaliana |
| (ATSYP132, SYP132, AT5G08080) protein fused to GFP |
accumulates weakly in |
cell-division plane |
|
| SYP132::GFP-SYP132 |
rescued partially |
knolle mutant |
|
| phragmoplast |
is actively guided toward |
cortical position marked by MYA1-GFP |
Arabidopsis thaliana |
| F-actin |
function in microtubule organization in |
central spindle at late anaphase and telophase |
Nicotiana tabacum |
| (ASG6, CRK2, AT1G70520) mutants |
exhibit |
compacted phragmoplast microtubules without recognizable array formation |
Zea mays |
| KEULE-KNOLLE interaction |
occurred at |
cell division plane |
Arabidopsis thaliana |
| (EXO84B, AT5G49830) exocyst subunit |
localizes to |
nascent cell plates and postcytokinetic cross walls |
|
| exocyst |
is required for |
secretory vesicle-mediated abscission |
Homo sapiens |
| (AtTRS120, TRAPPC9, TRS120, VAN4, AT5G11040) signal |
began to decrease at |
cell plate |
|
| overlap between TRAPPII and exocyst complexes |
occurred at |
phragmoplast assembly stage at the onset of cytokinesis |
|
| SOK3-mGFP |
localizes to |
plasma membrane of newly formed cell walls post-division |
Nicotiana benthamiana |
| cell plate-associated proteins |
include |
KNOLLE |
|
| clathrin light chain (CLC) |
is localized to |
cell plate |
Arabidopsis thaliana |
| plasma membrane lipid order |
does not change during |
cytokinesis |
Arabidopsis thaliana |
| (ADL1, ADL1A, AG68, DL1, DRP1A, RSW9, AT5G42080) |
may modulate |
membrane lipid order at the cell plate |
|
| MYOXI-I |
does not show co-localization with |
newly forming cell plate |
Arabidopsis thaliana |
| division plane |
is not determined by |
preprophase band |
|
| off-centre phragmoplast positioning |
probably accounts for |
incomplete callosic walls in mutant tio-3 spores |
|
| (KINESIN-12A, PAKRP1, AT4G14150) and (KINESIN-12B, PAKRP1L, AT3G23670) |
play |
redundant roles in cytokinesis |
|
| Golgi stacks |
do not orient towards |
cell plate |
Arabidopsis thaliana |
| vacuole system |
undergoes major changes in |
organization, size, and volume |
Arabidopsis thaliana |
| SNARE complex comprising Qa-SNARE KNOLLE, Qb,c-SNARE (ATSNAP33, ATSNAP33B, SNAP33, SNP33, AT5G61210) and R-SNARE (AT VAMP7B, ATVAMP721, VAMP721, VAMP7B, AT1G04750) (or (ATVAMP722, SAR1, VAMP722, AT2G33120) ) |
mediates |
cell plate formation by vesicle fusion during cytokinesis |
Arabidopsis thaliana |
| AtTAN::YFP ring |
narrows to become sharper and more punctate during |
cytokinesis |
Arabidopsis thaliana |
| interaction of (FU, TIO, AT1G50240) with Kinesin-12 |
depends largely on |
C-terminal domain consisting of four ARM/HEAT repeats and two highly conserved sequence motifs |
Arabidopsis thaliana |
| ProMSP1-TFARM4-3MYC |
showed mean frequency of |
46% aberrant pollen |
|
| bundling activity of (ATMAP65-3, MAP65-3, PLE, AT5G51600) |
is one of |
two cellular devices that operate to maintain interdigitating anti-parallel microtubules |
Arabidopsis thaliana |
| (FU, TIO, AT1G50240) and other potential substrates located in phragmoplast midzone |
could include |
MAP65 family members or regulators of NACK-PQR pathway |
Arabidopsis thaliana |
| (FU, TIO, AT1G50240) kinase activity |
is required for |
(FU, TIO, AT1G50240) role in phragmoplast expansion |
|
| ProMSP1-TFFR-3MYC (Fax random disruption) |
was not capable of rescuing |
+/tio-3 mutant phenotype |
|
| Kinesin-12 members |
are required to keep |
phragmoplast midzone discrete |
Arabidopsis thaliana |
| microtubule structures |
includes |
phragmoplast |
Arabidopsis thaliana |
| KNOLLE transgene |
is necessary to rescue |
cytokinesis defects |
Arabidopsis thaliana |
| cytokinesis defects in double mutant |
inherent in |
double mutant |
Arabidopsis thaliana |
| phragmoplast |
is guided towards |
cortical division site |
|
| sterols |
accumulate in |
cytokinetic furrow of Lytechinus pictus |
Lytechinus pictus |
| cpi1-1 mutant |
displays similar GP values for |
cell plate and plasma membrane |
Arabidopsis thaliana |
| lovastatin (lov) treatment |
induces |
lateral KNOLLE mis-localization |
Arabidopsis thaliana |
| wild-type |
exhibits higher mean GP value for |
cell plate than for plasma membrane |
Arabidopsis thaliana |
| NbRabE1 deficiency |
leads to |
defective guard cell cytokinesis |
Nicotiana benthamiana |
| (ATSYP132, SYP132, AT5G08080) |
can substitute for |
KNOLLE |
|
| KN complex formation with SNARE partners |
involves |
VAMP |
Arabidopsis thaliana |
| (ATRABA1B, BEX5, RAB11, RABA1b, AT1G16920) localization to cleavage furrow |
depends on |
bru |
Drosophila melanogaster |
| BUB3;1:GFP |
localized to |
middle part of the phragmoplast in telophase |
Arabidopsis thaliana |
| (AtMAD1, MAD1, NES1, AT5G49880) |
accumulates at |
phragmoplast |
Arabidopsis thaliana |
| OPL2-mCherry |
localizes to |
plasma membrane adjacent to new cell walls |
Nicotiana benthamiana |
| MyoXI-4KO mutant cells expressing MYA1-TagRFP |
formed |
regular, transverse cell plate parallel to others in same cell layer |
Arabidopsis thaliana |
| F-actin filaments |
were similar in |
MyoXI-4KO mutant, wild-type, and MYA1-GFP expressing mutant cells |
|
| mock-treated cells |
confine MYA1 signal within approximately 2% of |
cell perimeter |
|
| (POK1, AT3G17360) |
captures |
microtubules emanating from the phragmoplast edge |
|
| phragmoplast microtubules |
guide delivery of |
TGN-derived vesicles |
Arabidopsis thaliana |
| TRAPPII complex |
is required to mediate |
membrane addition during initial growth phase of cytokinesis |
|
| (ATSYP132, SYP132, AT5G08080) accumulation at plane of cell division |
depends on |
de novo synthesis during late-G2 to M phase |
Arabidopsis thaliana |
| KNOLLE |
interacts with |
(ATSYP71, SYP71, AT3G09740) |
Arabidopsis thaliana |
| knolle mutant |
displays |
cytokinesis-defective phenotype |
Arabidopsis thaliana |
| co-localization studies in planta |
strengthened connections during |
cytokinesis |
Arabidopsis thaliana |
| ring of prominent cytoskeletal assemblies |
receives |
expanding phragmoplast |
Arabidopsis thaliana |
| F-actin network |
is required for |
(ATMYA1, MYA1, XI-1, AT1G17580) localization |
|
| actin-microtubule interaction |
may be equally important for |
plant cytokinesis |
|
| sterols |
accumulate in |
cytokinetic furrow of Strongylocentrotus drobachiensis |
Strongylocentrotus drobachiensis |
| KNOLLE |
is constrained to |
cell plate and endomembrane compartments |
Arabidopsis thaliana |
| multivesicular bodies |
increase 4x in volume during |
late cytokinesis |
Arabidopsis thaliana |
| plant cells |
partition by forming |
cell plates |
|
| depolymerisation of microtubules in the centre of the phragmoplast |
enables |
phragmoplast expansion |
|
| (FU, TIO, AT1G50240) mutants |
show failure of |
callosic cell-plate expansion |
|
| (FU, TIO, AT1G50240) |
has essential role in |
regulated expansion of the phragmoplast and cell plate |
|
| ProMSP1-TFΔC without 3-myc tag |
failed to complement |
+/tio-3 mutant phenotype |
|
| (ATNACK1, HIK, NACK1, AT1G18370) and (ATNACK2, NACK2, TES, AT3G43210) kinesins |
trigger |
NACK-PQR pathway in somatic cells |
Arabidopsis thaliana |
| (ATMAP65-3, MAP65-3, PLE, AT5G51600) |
has critical role in |
interaction between interdigitating microtubules |
Arabidopsis thaliana |
| anchoring activity of Kinesin-12 |
is one of |
two cellular devices that operate to maintain interdigitating anti-parallel microtubules |
Arabidopsis thaliana |
| cell plate lipid order |
progressively decreases during |
cytokinesis |
Arabidopsis thaliana |
| cell plate |
represents |
dynamic, high lipid-order membrane domain |
Arabidopsis thaliana |
| EXO84b-GFP fluorescence in expanding cell plates |
was present as |
diffuse cloud around the plate |
|
| BUB3;1 and BUB3;2 |
localize to |
phragmoplast |
Arabidopsis thaliana |
| (ATSYP132, SYP132, AT5G08080) tam mutant embryos |
display mutant phenotypes nearly indistinguishable from |
knolle mutant embryos |
Arabidopsis thaliana |
| ancient SYP132-containing SNARE complexes |
contribute to |
cytokinesis |
Arabidopsis thaliana |
| KNOLLE |
promotes |
fusion of membrane vesicles delivered to the midplane between adjacent nuclei |
Arabidopsis thaliana |
| knolle (ATSYP132, SYP132, AT5G08080) tam double mutant |
almost completely abolishes |
cytokinesis |
|
| (CDC2B, CDKB1;1, AT3G54180) |
has been identified at |
phragmoplast array |
Arabidopsis thaliana |
| MYA1-GFP |
continuously marks |
CDS until phragmoplast reaches the site |
Arabidopsis thaliana |
| phragmoplast microtubules |
have direct connection to |
cell division site (CDS) |
|
| wortmannin-treated cells |
had no |
binucleate cells |
Arabidopsis thaliana |
| cortical division site |
guides |
expanding phragmoplast |
|
| (EMB3013, RUK, AT5G18700) K33W and D121A adult plants |
displayed |
cytokinesis defects in flowers |
Arabidopsis thaliana |
| (AUG8, QWRF8, AT4G30710) |
was not detected on |
phragmoplast MTs |
Arabidopsis thaliana |
| C-terminal ARM/HEAT repeat domain of (FU, TIO, AT1G50240) |
is required for |
(FU, TIO, AT1G50240) function in cytokinesis |
Arabidopsis thaliana |
| ProMSP1-TF-3MYC (full-length (FU, TIO, AT1G50240) with 3× myc tag) |
fully complemented |
+/tio-3 phenotype |
|
| microtubule-based structures |
play an essential role in |
cytokinesis |
|
| CLC2-GFP |
cell-plate localization is unaffected in |
cpi1-1 mutant |
Arabidopsis thaliana |
| (CLC2, AT2G40060) |
co-localizes with |
sterols at the cell plate in roots |
Arabidopsis thaliana |
| Golgi stacks |
concentrate around the periphery of |
growing cell plate |
Arabidopsis thaliana |
| clathrin-coated vesicles (CCVs) |
increase 4x in number during |
late cytokinesis |
Arabidopsis thaliana |
| COPI complex |
is required for |
cell plate formation |
Arabidopsis thaliana |
| lipid order |
may have feedback with |
dynamin-related proteins function during cytokinesis |
Arabidopsis thaliana |
| sterols |
accumulate at |
contractile actin ring in fission yeast and sea urchins during cytokinesis |
Schizosaccharomyces pombe; Strongylocentrotus purpuratus |
| increased cytosol volume |
accommodates |
phragmoplast microtubule array |
Arabidopsis thaliana |
| pan1 mutants |
exhibited no defects in |
cell plate formation |
Zea mays |
| sec24a-2 mutant |
shows normal |
cytokinesis |
Arabidopsis thaliana |
| disruption of secretion in plants |
can result in |
cytokinesis defects |
|
| endosidin 7 (ES7) |
is an inhibitor of |
cell plate formation |
|
| cell wall formation |
is altered under high and extreme heat stress |
cross-shaped cell wall between separated nuclei |
Arabidopsis thaliana |
| pan mutant |
analysis did not reveal unique role for PAN1 in |
cell plate formation |
Zea mays |
| ANP subfamily of MAP3K (mitogen-activated protein kinase kinase kinase) |
have been shown to functionally interact with |
proteins required for cytokinesis |
Arabidopsis thaliana |
| PAN1 |
is enriched at |
developing cell plates |
Zea mays |
| phragmoplast |
expands |
phragmoplast expansion |
|
| PAN2 |
is not detectable at |
cell plates |
Zea mays |
| loss of (ANQ1, ATMKK6, MKK6, SUMM4, AT5G56580) and (ATMPK4, MAPK4, MPK4, AT4G01370) |
leads to |
severe defects in cytokinesis |
Arabidopsis thaliana |
| phragmoplast F-actin |
has unclear role in |
cell plate formation |
|
| cell plate |
fuses with |
parental plasma membrane |
|
| future research into callose synthase role in cytokinesis |
will lead to |
comprehensive understanding of cytokinesis |
Arabidopsis thaliana |
| (ATN, ATTAN, TAN1, AT3G05330) (AIR9, AT2G34680) plants expressing -∆II-YFP |
had 69.7% of phragmoplast angles between 80° and 100° |
normal phragmoplast orientation |
Arabidopsis thaliana |
| new cell wall |
is formed |
cell wall formation |
|
| (ANQ1, ATMKK6, MKK6, SUMM4, AT5G56580) and (ATMPK4, MAPK4, MPK4, AT4G01370) |
function downstream of ANPs to regulate |
cytokinesis |
Arabidopsis thaliana |
| ANPs |
function upstream of |
(ANQ1, ATMKK6, MKK6, SUMM4, AT5G56580) |
Arabidopsis thaliana |
| lovastatin (lov) treatment |
reduces |
cell-plate membrane lipid order |
Arabidopsis thaliana |
| mitotic kinesins |
exhibit localization patterns at |
phragmoplast distal ends |
|
| phragmoplast |
functions as |
dynamic scaffold for assembly of new cell wall (cell plate) |
|
| cell plate |
forms |
network of interconnected tubules |
|
| early cell plate |
expands laterally by fusing with |
other arriving vesicles |
|
| proteins regulating vesicle targeting, fusion, and fission |
participate in |
distinct phases of cell plate formation |
|
| pan1 mutant |
shows no difference in |
phragmoplast-associated F-actin |
Zea mays |
| (ATMPK4, MAPK4, MPK4, AT4G01370) |
physically interacts with |
(ATMAP65-1, MAP65-1, AT5G55230) |
Arabidopsis thaliana |
| phragmoplast microtubules |
have essential role in |
transporting vesicles to cell plate |
|
| cytokinesis in many eukaryotes other than plants |
is accomplished by |
contraction of the cleavage furrow at the division plane |
|
| RABA2A vesicles |
are involved in neither |
cell plate maturation |
Arabidopsis thaliana |
| lipid composition, TGN-mediated trafficking, and callose deposition |
are all contributing factors to |
cell plate formation |
Arabidopsis thaliana |
| homotypic vesicle fusion |
sets up |
early cell plate |
|
| flufenacet treatment |
led to |
ectopic phragmoplast formation at the mature cell plate |
Arabidopsis thaliana |
| (TPLATE, AT3G01780) |
functions in |
cell plate formation |
|
| Filamenting temperature-sensitive Z (FtsZ) |
is essential for |
cytokinesis |
|
| cell plate |
eventually forms |
continuous sheet perforated by plasmodesmata |
|
| cytokinesis in plants |
requires |
de novo secretion of vesicles to the division plane |
|
| (ANP1, MAPKKK1, NP1, AT1G09000) (ANP2, MAPKKK2, NP2, AT1G54960) (ANP3, AtANP3, MAPKKK12, NP3, AT3G06030) triple mutant |
cannot be obtained because of |
lethality |
Arabidopsis thaliana |
| phragmoplast |
builds |
cell plate between the newly divided nuclei |
|
| STD1 |
is homolog of |
(PAKRP2, AT4G14330) |
Oryza sativa; Arabidopsis thaliana |
| mitotic kinesins |
exhibit localization patterns at |
phragmoplast midzone |
|
| (ATVAMP722, SAR1, VAMP722, AT2G33120) |
is involved in |
cell plate formation |
|
| central spindle |
is composed of |
aligned microtubules and microfilaments |
|
| phragmoplast guidance |
is a mechanism for |
cell plate insertion at cell cortex |
|
| actomyosin system and microtubules |
execute |
cytokinesis in spatiotemporally regulated manner |
|
| microtubules |
become anchored when |
two motor assemblies join each other |
|
| cell plate growth |
is dependent upon |
phragmoplast formation |
Arabidopsis thaliana |
| HINKEL/ (ATNACK1, HIK, NACK1, AT1G18370) /OsNACK1 |
is essential for |
completion of cell plate formation |
|
| std1 mutant |
had presence of |
many multinucleate cells |
Oryza sativa |
| (At-SCL28, SCL28, AT5G18810) |
modulates |
phragmoplast activity |
Arabidopsis thaliana |
| Transport Protein Particle II (TRAPPII) |
has been implicated in |
cytokinesis in both the plant and animal kingdoms |
|
| (AtTRS120, TRAPPC9, TRS120, VAN4, AT5G11040) and (SEC6, AT1G71820) |
showed different peak fluorescence localization |
cell plate versus cross walls |
|
| KNOLLE protein in exo84b-2 mutants |
prematurely appeared as punctate stain at |
cell plate |
Arabidopsis thaliana |
| OPL2-mCherry |
localizes to |
newly forming cell plate |
Nicotiana benthamiana |
| TRAPPII complex |
may be required for |
cleavage furrow ingression |
Drosophila melanogaster |
| membrane-fusion machinery |
appears to be |
more complex |
Arabidopsis thaliana |
| KNOLLE-like proteins |
might only have acquired |
novel essential role in angiosperms |
|
| (KINESIN-12A, PAKRP1, AT4G14150) and (KINESIN-12B, PAKRP1L, AT3G23670) |
function exclusively in |
midzones |
|
| absence of (ATMAP65-3, MAP65-3, PLE, AT5G51600) |
may increase |
flux of tubulin through plus ends |
Arabidopsis thaliana |
| association of (FU, TIO, AT1G50240) with the expanding phragmoplast ring |
demonstrates |
requirement for (FU, TIO, AT1G50240) in the phragmoplast during cell plate expansion |
Arabidopsis thaliana |
| decreased (FU, TIO, AT1G50240) protein levels in roots of -RNAi plants |
supports conclusion that |
(FU, TIO, AT1G50240) has an essential role in somatic cell cytokinesis |
Arabidopsis thaliana |
| phragmoplast |
expands to attach |
cell plate to parental wall at division site |
|
| cytokinesis defects of runkel (EMB3013, RUK, AT5G18700) mutants |
suggest that role of RUK in cytokinesis might be related to |
microtubule-destabilizing activity of (ATNACK1, HIK, NACK1, AT1G18370) kinesin |
Arabidopsis thaliana |
| (ATNACK1, HIK, NACK1, AT1G18370) and NPK1 |
localize to |
plus end of phragmoplast microtubules |
Arabidopsis thaliana |