| CALCIUM-DEPENDENT PROTEIN KINASE32 (ATCPK32, CDPK32, CPK32, AT3G57530) |
phosphorylates |
(ATCESA3, ATH-B, CESA3, CEV1, ELI1, IXR1, MRE1, AT5G05170) |
Arabidopsis thaliana |
| CPK32ΔC K96M transgenic plants |
had |
reduced cellulose content |
Arabidopsis thaliana |
| phosphorylation |
may influence |
endocytosis of CESAs |
|
| CSCs |
cycle between |
Golgi apparatus, small CESA compartment or microtubule-associated cellulose synthase compartments (SmaCCs/MASCs), and plasma membrane |
|
| phosphorylation |
could result in conformational changes that induce disengagement of CSCs from |
cortical microtubules |
|
| cellulose |
is synthesized by |
multimeric rosettes |
|
| brittle-culm phenotype in bc3 mutant |
was attributed to |
cellulose deficiency |
Oryza sativa |
| phosphorylation and dephosphorylation of (ATCESA3, ATH-B, CESA3, CEV1, ELI1, IXR1, MRE1, AT5G05170) |
regulates |
stability of CSCs |
|
| (SHOU4L, AT1G16860) 8SD transgenic lines |
suggest the possibility of |
increased cellulose content |
|
| protein kinases |
phosphorylate |
cellulose synthases (CESAs) |
Arabidopsis thaliana |
| cellulose synthetic pathway |
changed significantly following |
ethylene treatment of rose petals |
|
| SlTRM3/4 |
could impact interaction with |
cellulose synthase 3 in tomato |
Solanum lycopersicum |
| (ATCPK32, CDPK32, CPK32, AT3G57530) |
interacts with |
catalytic domain of (ATCESA3, ATH-B, CESA3, CEV1, ELI1, IXR1, MRE1, AT5G05170) (CESA3CD) |
Arabidopsis thaliana |
| (ATCESA2, ATH-A, CESA2, AT4G39350) (CESA5, MUM3, AT5G09870) (CESA6, E112, IXR2, PRC1, AT5G64740) and (CESA09, CESA9, AT2G21770) in Arabidopsis |
contribute to |
cellulose synthesis for primary cell wall |
Arabidopsis thaliana |
| RhCesA2 |
is |
homolog of Arabidopsis (ATCESA2, ATH-A, CESA2, AT4G39350) |
Rosa sp.; Arabidopsis thaliana |
| (ATCESA7, CESA7, IRX3, MUR10, AT5G17420) mutant |
indicates |
reduction in cellulose content without evidence of altered cellulose structure |
Arabidopsis thaliana |
| stunted growth of hypocotyls in (ATCESA2, ATH-A, CESA2, AT4G39350) mutant |
is due to |
reduced cell expansion caused by defect in cellulose synthesis |
Arabidopsis thaliana |
| CPK32ΔC K96M transgenic plants |
had reduced |
cellulose content |
|
| delta TR (ΔTR) |
remains unchanged once |
cellulose has been synthesized |
|
| CSC motility in control seedlings |
had average velocity of |
311 ± 233 nm min−1 |
Arabidopsis thaliana |
| (ATCPK32, CDPK32, CPK32, AT3G57530) |
regulates |
CSC dynamics and cellulose synthesis |
Arabidopsis thaliana |
| (GLC, AT1G65450) decrease in cesa5-1 nonadherent mucilage |
is consistent with |
decrease in mucilage cellulose synthesis |
Arabidopsis thaliana |
| SALT-OVERLY SENSITIVE 5 (FLA4, SOS5, AT3G46550) |
does not seem to be required for |
arrangement or movement of CELLULOSE SYNTHASE 5 (CESA5, MUM3, AT5G09870) in seed coat epidermal cells |
Arabidopsis thaliana |
| number of CSCs in compartments |
is expected to undergo tight regulation |
|
|
| YFP-CESA6 in control seedlings |
had 75.4% remaining after 36 h cycloheximide treatment |
protein stability |
Arabidopsis thaliana |
| (ATCPK32, CDPK32, CPK32, AT3G57530) |
regulates stability of via phosphorylation of |
cellulose synthase complexes (CSCs) |
|
| AtTRM4 |
potentially through direct interaction with |
CELLULOSE SYNTHASE 3 (ATCESA3, ATH-B, CESA3, CEV1, ELI1, IXR1, MRE1, AT5G05170) |
Arabidopsis thaliana |
| protein biochemistry, genetics, and live-cell imaging |
used to reveal |
role of calcium-dependent protein kinase32 (ATCPK32, CDPK32, CPK32, AT3G57530) in regulation of cellulose biosynthesis |
Arabidopsis thaliana |
| CALCIUM-DEPENDENT PROTEIN KINASE32 (ATCPK32, CDPK32, CPK32, AT3G57530) |
interacts with |
(ATCESA3, ATH-B, CESA3, CEV1, ELI1, IXR1, MRE1, AT5G05170) |
Arabidopsis thaliana |
| CPK32ΔC K96M transgenic plants |
had |
decreased CSC motility |
Arabidopsis thaliana |
| synthesis of tree-ring cellulose |
does not cause a significant modification of |
photosynthetic delta C13 signature |
|
| cellulose accumulated during growth in tree rings |
has no turnover |
afterwards |
|
| rosettes |
are visualized at |
plasma membrane |
|
| stunted growth, curved stems and fragile branches |
are reminiscent of |
(STL1, AT2G41770) (STL2, AT3G57420) double mutants exhibiting stunted growth with decreased cellulose |
|
| CSCs |
are internalized via |
clathrin-mediated endocytosis |
|
| internalized CESAs |
can be sorted to |
SmaCCs/MASCs for endocytic recycling |
|
| CPK32ΔC K96M |
sequesters |
(ATCESA3, ATH-B, CESA3, CEV1, ELI1, IXR1, MRE1, AT5G05170) |
|
| phosphorylation of two non-conserved residues of (ATCESA7, CESA7, IRX3, MUR10, AT5G17420) |
has been associated with |
proteosome-dependent degradation of (ATCESA7, CESA7, IRX3, MUR10, AT5G17420) |
|
| CPK32ΔC K96M |
does not impact |
assembly of CSCs |
Arabidopsis thaliana |
| deficiency in phosphorylation of (ATCESA3, ATH-B, CESA3, CEV1, ELI1, IXR1, MRE1, AT5G05170) |
may cause |
impact on stability of CSCs |
Arabidopsis thaliana |
| CPK32ΔC K92M transgenic plants |
were comparable to |
wild type |
Arabidopsis thaliana |
| overexpression of functionally defective (ATCPK32, CDPK32, CPK32, AT3G57530) variant |
leads to decreased |
motility of cellulose synthase complexes (CSCs) |
Arabidopsis thaliana |
| CPK32ΔC K96M overexpression |
impacts |
stability of CSCs |
Arabidopsis thaliana |
| phosphorylation |
plays important role in regulation of |
anisotropic cell expansion |
|
| RhCesA2 |
has closest homolog |
(ATCESA2, ATH-A, CESA2, AT4G39350) in Arabidopsis |
|
| CALCIUM-DEPENDENT PROTEIN KINASE32 (ATCPK32, CDPK32, CPK32, AT3G57530) |
regulates |
cellulose biosynthesis |
Arabidopsis thaliana |
| CESA1CD |
shares protein sequence identity with |
CESA3CD |
Arabidopsis thaliana |
| CSC phosphorylation |
is shown to regulate |
motility and bidirectional movement of CSC in primary cell walls |
Arabidopsis thaliana |
| cellulose synthase genes |
primarily govern |
cellulose synthetic pathway |
|
| overexpression of functionally defective (ATCPK32, CDPK32, CPK32, AT3G57530) variant |
leads to reduced |
crystalline cellulose content in etiolated seedlings |
Arabidopsis thaliana |
| (ATCESA3, ATH-B, CESA3, CEV1, ELI1, IXR1, MRE1, AT5G05170) T672 |
is located at |
class-specific region (CSR) |
|
| CPK32ΔC K96M transgenic plants |
had reduced |
CSC motility |
|
| (ATCESA3, ATH-B, CESA3, CEV1, ELI1, IXR1, MRE1, AT5G05170) S671 and T672 mutation to alanine |
is predicted to prevent regulation by |
CPKs |
|
| Golgi-localized CSCs |
were retained after |
50 hours of 1 mM cycloheximide treatment |
|
| cellulose synthase inhibitors such as isoxaben |
had a role in |
identification of cellulose synthase subunits |
|
| β-(1,4)-D-glucan chains |
are synthesized at |
plasma membrane |
|
| tree-ring cellulose |
is less affected by |
postphotosynthetic fractionation processes, which include transport of photosynthetic products and cellulose biosynthesis |
|
| (ATCESA8, CESA8, IRX1, LEW2, AT4G18780) mutant |
indicates |
reduction in cellulose content without evidence of altered cellulose structure |
Arabidopsis thaliana |
| genetic association between SNP24 in PtREV and wood α-cellulose content |
indicates |
possible regulatory function for (AVB1, IFL, IFL1, REV, AT5G60690) in coordinating cellulose biosynthesis during differentiation of cells with secondary cell wall |
Populus trichocarpa |
| phosphorylation |
regulates |
stability of cellulose synthase complexes (CSCs) |
Arabidopsis thaliana |
| CPK32ΔC K96M |
was not able to impact cellulose production when introduced to |
cpk32-1 null mutant |
|
| internalized CESAs |
can be sorted to |
vacuole for degradation |
|
| (GLC, AT1G65450) levels in cesa5-1 nonadherent mucilage |
were significantly decreased |
|
Arabidopsis thaliana |
| (CESA10, AT2G25540) family |
is |
Poaceae specific |
|
| mutant cesa proteins |
allow |
assembly of the cellulose synthase complex |
Arabidopsis thaliana |
| different effects of mutating members of different CESA classes |
are consequence of |
different catalytic activity and influence on overall rate of cellulose synthesis |
Arabidopsis thaliana |
| cellulose quantities in cesa5-1 seeds |
were significantly reduced in |
compared with wild-type |
Arabidopsis thaliana |
| mutant cesa proteins |
retain |
sufficient structural integrity |
Arabidopsis thaliana |
| (GLC, AT1G65450) values for cesa5-1 sos5-2 double mutant |
were not significantly different from |
either single mutant |
Arabidopsis thaliana |
| volcano-shaped cytoplasmic columns |
are circled by |
cellulose synthases |
Arabidopsis thaliana |
| cellulose |
is made at |
plasma membrane |
|
| ZmBk2L3 |
was not affected |
maize cultures analyzed in this research |
Zea mays |
| H12 habituated cultures |
induces expression of |
ZmCesA8 |
Zea mays |
| CESA complexes |
use as activated sugar donor |
UDP-glucose (UDP-Glc) |
|
| (CESA10, AT2G25540) group |
lacks |
(AT-GT2, GT2, AT1G76890) characteristic functional motif QXXRW |
|
| enzyme complex |
synthesizes |
cellulose |
|
| amount of (GLC, AT1G65450) in sos5-2 seeds |
was not significantly different from |
wild-type |
Arabidopsis thaliana |
| kymographs of GFP-CESA5 in wild-type and sos5-2 backgrounds |
appeared |
similar |
Arabidopsis thaliana |
| ZmCesA4 |
no mRNA detected in |
maize cells |
Zea mays |
| VIGS-NbCOBRA |
does not show major reduction of |
crystalline cellulose in stems |
|
| rosette-like structures and detergent-soluble catalytic subunit |
together represent |
functional cellulose synthase machinery |
Vigna angularis |
| cellulose synthase superfamily |
contains |
CesA subfamily |
|
| cellulose microfibrils |
form |
load-bearing structural network of the wall |
|
| (ATFLA11, FLA11, AT5G03170) (AtFLA12, FLA12, AT5G60490) double mutant |
has |
reduced cellulose deposition |
Arabidopsis thaliana |
| Altered GIPC headgroup glycosylation |
results in a decrease in |
cellulose content |
|
| radially swollen (rsw) mutants |
have |
root radial swelling at restrictive temperatures |
Arabidopsis thaliana |
| sos5-2 nonadherent mucilage |
had levels of Glc that were not statistically different from |
wild-type |
Arabidopsis thaliana |
| SALT-OVERLY SENSITIVE 5 (FLA4, SOS5, AT3G46550) |
has |
limited, if any, role in amount of cellulose synthesized in seeds |
Arabidopsis thaliana |
| (ATCESA8, CESA8, IRX1, LEW2, AT4G18780) |
is involved in |
secondary cell wall biosynthesis |
Arabidopsis thaliana |
| cortical microtubule cytoskeleton |
provides a dynamic scaffold for |
targeting of cellulose-producing enzymes |
|
| (FLA4, SOS5, AT3G46550) regulation of mucilage adherence |
may involve |
cellulose biosynthesis |
Arabidopsis thaliana |
| cellulose biosynthesis |
has been demonstrated in |
cyanobacteria Anabaena spp. and Nostoc punctiforme |
Anabaena spp.; Nostoc punctiforme |
| phosphorylated plasma membrane-bound forms of sucrose synthase (SuSy) |
are implicated in carbon supply for |
cellulose production |
|
| cellulose synthase activity |
is observed in |
detergent-resistant membranes (DRMs) |
|
| changes in cellulose microfibril structure |
reflect differences in |
relative proportions of primary and secondary cell walls |
Arabidopsis thaliana |
| CSC motility in GFP-CESA3 S671A T672A plants |
had 27.8% reduction to |
135 ± 85 nm min−1 average velocity |
Arabidopsis thaliana |
| cellulose synthesis |
occurs solely at |
plasma membrane |
|
| cellulose deficiency of bc3 or (ADL1, ADL1A, AG68, DL1, DRP1A, RSW9, AT5G42080) |
remains unclear whether it results directly from |
perturbations in CESA trafficking |
|
| cellulose biosynthesis machinery |
forms |
rosettes |
|
| cellulose biosynthesis machinery |
forms |
cellulose synthase (CESA) complexes |
|
| H12 habituated cultures in stationary phase |
continues to induce expression of |
ZmCesA7 |
Zea mays |
| plasma membrane-associated protein fractions of Azuki bean epicotyls |
exhibit |
in vitro β-1,4-glucan synthase activity |
Vigna angularis |
| granulates |
contained |
sucrose synthase (SuSy) homolog |
Vigna angularis |
| (AtUGP1, UGP, UGP1, AT3G03250) (AtUGP2, UGP2, AT5G17310) double mutants |
displayed |
growth defects and down-regulation of different CesA transcripts |
Arabidopsis thaliana |
| only CELLULOSE SYNTHASE 5 (CESA5, MUM3, AT5G09870) |
significantly affects |
crystalline cellulose quantities |
Arabidopsis thaliana |
| cello-oligosaccharides |
can serve as effectors or primers for |
cellulose synthase |
|
| AtCesA4 (IRX5: irregular xylem 5) |
is essential for |
secondary cell wall formation |
Arabidopsis thaliana |
| CesA and cellulose synthase activity |
is enriched in |
detergent-resistant membranes |
|
| positive effect on cellulose production |
appeared to be dependent on |
sucrose synthase (SuSy) isoform used |
Populus trichocarpa |
| CESA complexes |
are organized as |
hexamers |
|
| H12 cultures |
induced the expression of |
ZmCesA7 |
Zea mays |
| ZmCesA7 |
continued to be induced |
expression |
Zea mays |
| es20r1 – es20r15 mutants |
show reduced sensitivity to |
ES20-3 treatment |
Arabidopsis thaliana |
| isoxaben |
docks to |
transmembrane regions close to extracellular side of plasma membrane in (CESA6, E112, IXR2, PRC1, AT5G64740) |
Arabidopsis thaliana |
| cellulose biosynthesis inhibitors (CBIs) |
are useful for |
studying the mechanism of cellulose biosynthesis |
|
| increased binding affinity to (CESA6, E112, IXR2, PRC1, AT5G64740) |
may explain |
increased inhibitory potency of ES20-1 |
|
| important motifs required for cellulose synthesis |
are highly conserved among |
CESAs across kingdoms |
|
| electron microscopy |
observed |
granulates of 9.5–10 nm in size |
Vigna angularis |
| dichlobenil (DCB) |
inhibits |
cellulose biosynthesis |
|
| ZmCesA7 and 8 |
grouped with |
Arabidopsis thaliana CesA proteins involved in the synthesis of cellulose in the primary to secondary cell wall transition |
Zea mays; Arabidopsis thaliana |
| expression of maize CesA genes most closely related to (ATCESA3, ATH-B, CESA3, CEV1, ELI1, IXR1, MRE1, AT5G05170) |
was not altered |
ZmCesA5 |
Zea mays |
| connection between microtubules and cellulose synthesis |
has been proved in recent years |
experimental evidence |
|
| sitosterol-β-glucoside |
is the major sterol in |
detergent-resistant membranes |
|
| granular particles |
are catalytic subunits for |
cellulose biosynthesis |
Vigna angularis |
| cellulose synthase activity in detergent-resistant membranes |
is possibly associated with |
57-kDa proteolytic CesA version |
|
| β-1,4-glucan chain synthase activity |
could only be detected in detergent-insoluble fractions after addition of |
detergent-soluble fractions |
Vigna angularis |
| exact number, identity, and arrangement of CesA subunits in the CesA complex |
still remains unknown |
CesA complex structure |
|
| xyloglucan |
prevents |
fasciation of cellulose |
|
| removal of DCB from culture media |
reverses reduction in |
cellulose content |
Zea mays |
| some SuSys |
have been localized to |
plasma membrane and cell walls |
|
| cellulose synthesizing machinery |
interacts with |
cytoskeletal systems |
|
| H12 cell walls |
present a reduction of more than 50% in |
cellulose content in the stationary phase |
Zea mays |
| sterol synthesis inhibitors |
cause defects in |
cellulose synthesis and deposition |
|
| sucrose synthase (SuSy) |
is not a compulsory requirement for |
cellulose production |
Arabidopsis thaliana |
| ES20 and ES20-1 |
dock to |
catalytic site of (CESA6, E112, IXR2, PRC1, AT5G64740) |
Arabidopsis thaliana |
| cortical microtubules |
serve as tracks for |
cellulose synthase complexes |
|
| H12 habituated cultures |
induces expression of |
ZmCesA7 |
Zea mays |
| VIGS-NbIRX8 |
does not show major reduction of |
crystalline cellulose in stems |
|
| mutations in any one of the three CesAs |
disrupt |
cellulose synthesis |
|
| H12 maize cell cultures |
shows strong reduction in |
cellulose content |
Zea mays |
| DCB |
induces |
ZmCesA7 |
Zea mays |
| nitric oxide |
leads to the increase in |
cellulose content in primary cell walls |
Solanum lycopersicum |
| brassinosteroids (BRs) |
induce expression of |
CESA genes |
Arabidopsis thaliana |
| tobacco cells habituated to 1 μM DCB |
found a higher amount of the protein |
celA1 |
Nicotiana tabacum |
| overexpression of a cotton sucrose synthase (SuSy) gene in poplar |
supports the idea of direct connection between |
sucrose supply, its breakdown, and cellulose production through SuSy activity |
Populus trichocarpa |
| cellulose |
is synthesized at |
plasma membrane |
|
| isoxaben habituation in Arabidopsis |
does not appear to be mediated by |
functional redundancy within the CesA family |
Arabidopsis thaliana |
| H12 habituated cultures |
induces expression of |
ZmCesA3 |
Zea mays |
| β-1,4-glucanase association with cellulose synthase complex |
severs |
cellulose chains |
|
| (ATCESA2, ATH-A, CESA2, AT4G39350) (CESA5, MUM3, AT5G09870) (CESA6, E112, IXR2, PRC1, AT5G64740) and (CESA09, CESA9, AT2G21770) |
have partially redundant functions in |
primary cell wall formation |
Arabidopsis thaliana |
| cellulose |
is synthesized at |
plasma membrane |
|
| mutations in the two UDP-glucose:sterol glucosyltransferases, AtUGT80A2 and AtUGT80B1 |
did not affect |
cellulose biosynthesis |
|
| PtdCesA8 |
overexpression causes silencing of |
endogenous PtdCesA8 counterparts |
Populus tremuloides |
| transgenic trees with altered cellulose content and properties |
is expected to provide novel insights into |
cellulose biosynthesis and cross-talk between synthetic pathways for different cell wall polymers |
|
| expression of maize CesA genes most closely related to (ATCESA3, ATH-B, CESA3, CEV1, ELI1, IXR1, MRE1, AT5G05170) |
was not detected |
ZmCesA4 |
Zea mays |
| ZmCesA5 |
is, at least partially, replaced by |
ZmCesA7 |
Zea mays |
| isoxaben-habituated cells |
not only AtCesA3, but also AtCesA6 were down-regulated |
AtCESA6 |
Arabidopsis thaliana |
| overexpression of an UDP-glucose pyrophosphorylase (UGPase) in hybrid poplar |
resulted in increase in |
cell wall cellulose content |
Populus trichocarpa |
| endosidin20-1 (ES20-1) |
show inhibitory effect on |
bacterial cellulose synthesis |
|
| C17 |
docks to |
transmembrane pocket in (CESA6, E112, IXR2, PRC1, AT5G64740) |
Arabidopsis thaliana |
| 13 out of 15 ES20-insensitive mutants (except es20r5 and es20r13) |
showed reduced sensitivity to |
ES20-2 |
|
| ES20-1 |
has |
mean predicted binding affinity with (CESA6, E112, IXR2, PRC1, AT5G64740) of −8.7 kcal mol−1 |
|
| H12 cultures during the active growing phase |
induced the expression of |
ZmCesA1/2 |
Zea mays |
| partially removing ZmCesA5 from the rosettes |
maize cells would be able to grow in the presence of |
herbicide |
Zea mays |
| invertases |
were proposed to provide carbon for |
cellulose production in non-photosynthetic cells |
|
| cellulose |
is composed of |
long chains of β-1,4 linked glucose molecules |
|
| β-1,4-glucanase association with cellulose synthase complex |
corrects |
microfibril formation |
|
| cellulose synthase (CesA) complexes (CSCs) |
synthesizes |
cellulose |
|
| BcsB |
is |
periplasmic protein |
|
| ES20 analogs |
show |
cell swelling |
|
| cellulose catalytic synthesis process |
is conserved between |
plants and bacteria |
|
| cortical microtubule cytoskeleton |
participates in |
coordinated secretion and internalization of cellulose synthase |
|
| endosidin20-1 (ES20-1) |
induces accumulation of |
cellulose synthase complexes at the Golgi apparatus |
|
| flupoxam |
is |
widely used tool to dissect the mechanism of cellulose biosynthesis |
|
| es20r1 – es20r15 mutants |
show reduced sensitivity to |
ES20 treatment |
Arabidopsis thaliana |
| ES20-1 |
more strongly inhibits |
root growth |
Arabidopsis thaliana |
| the other eight active ES20 analogs |
are less potent than |
ES20 |
|
| cellulose synthase interacting (CSI) proteins |
mediate |
co-alignment between microtubules and cellulose microfibrils |
|
| cellulose synthase inhibitors |
contributed to |
current understanding of cellulose biosynthesis |
|
| (GLC, AT1G65450) reduction in cesa5-1 seeds |
is consistent with |
CELLULOSE SYNTHASE 5 (CESA5, MUM3, AT5G09870) being involved in cellulose biosynthesis |
Arabidopsis thaliana |
| endosidin20-1 (ES20-1) |
inhibited |
bacterial cellulose biosynthesis |
|
| es20r1 and es20r5 |
showed reduced sensitivity to |
ES20-8 |
|
| ES20-6 and ES20-8 |
may inhibit cellulose synthesis using |
quite different mode of action than ES20 and the other seven active ES20 analogs |
|
| ES20-1 |
is likely to have |
the same mode of action as ES20 |
|
| ES20 and ES20-1 competing with the glucan chain to bind to the channel |
may interfere with |
cellulose biosynthesis |
|
| isoxaben |
may inhibit |
efficient translocation of cellulose across the PM |
|
| ES20 and ES20-1 |
inhibit cellulose synthesis in |
bacteria |
|
| advances in functional genomics |
have significantly improved |
understanding of cellulose biosynthesis processes |
|
| Cellulose synthase-associated proteins |
characterization of will advance knowledge of |
organization of cellulose microfibrils |
|
| expression of maize CesA genes most closely related to (ATCESA3, ATH-B, CESA3, CEV1, ELI1, IXR1, MRE1, AT5G05170) |
was not detected |
ZmCESA9 |
Zea mays |
| es20r1 – es20r15 mutants |
show reduced sensitivity to |
ES20-7 treatment |
Arabidopsis thaliana |
| ES20-1 |
interacts with |
CESA6c (central cytosolic domain of (CESA6, E112, IXR2, PRC1, AT5G64740) ) |
Arabidopsis thaliana |
| isoxaben |
inhibits |
cellulose biosynthesis |
|
| BRI1-GFP over-expression line |
has CESA7 expression not altered in young seedlings compared to |
wild type |
Arabidopsis thaliana |
| BRI1-GFP over-expression line |
has CESA8 expression not altered in young seedlings compared to |
wild type |
Arabidopsis thaliana |
| Arabidopsis thaliana (ANY1, AtCESA1, CESA1, RSW1, AT4G32410) |
involved in the synthesis of cellulose in |
primary cell wall |
Arabidopsis thaliana |
| UDP-glucose (UDP-Glc) |
can be synthesized by |
sucrose synthase (SuSy) |
|
| overexpression of a cotton sucrose synthase (SuSy) gene in poplar |
resulted in increased |
cellulose synthesis |
Populus trichocarpa |
| Arabidopsis quadruple (ASUS1, atsus1, SUS1, SUSY1, AT5G20830) (ATSUS2, SSA, SUS2, AT5G49190) (ATSUS3, SUS3, AT4G02280) (ATSUS4, SUS4, AT3G43190) mutant |
has production of cellulose not disturbed |
cellulose production |
Arabidopsis thaliana |
| ES20-1 to ES20-9 |
cause |
root growth inhibition |
Arabidopsis thaliana |
| regulated trafficking of cellulose synthase |
allows |
dynamic remodeling of cellulose synthase complex secretion |
|
| microtubules |
affect structure of |
newly deposited cellulose microfibrils |
|
| vesicle trafficking |
is facilitated by |
multiple CESA-interacting proteins and the cytoskeleton |
|
| transgenic plants expressing (CESA6, E112, IXR2, PRC1, AT5G64740) with missense mutations at the catalytic site |
show reduced sensitivity to |
ES20-9 treatment |
Arabidopsis thaliana |
| ES20-insensitive mutants |
showed reduced sensitivity to |
ES20-5 |
|
| predicted binding sites for CBIs |
are very close to |
amino acids that have been found to be essential for the inhibitory effects of the CBIs |
|
| fluorescently labeled CESA |
localizes to |
Small CESA compartments (SmaCCs) |
|
| ES20 |
may target |
transmembrane regions containing the cellulose-conducting channel |
|
| CELLULOSE SYNTHASE (CESA) |
is only active at |
plasma membrane |
|
| cortical microtubule array |
guides |
cellulose synthase complexes |
|
| endosidin20-1 (ES20-1) |
likely targets |
catalytic site of cellulose synthase 6 (CESA6, E112, IXR2, PRC1, AT5G64740) |
|
| ES20 and ES20-1 |
inhibit |
Komagataeibacter xylinus cell growth |
Komagataeibacter xylinus |
| acetobixan |
reduces |
cellulose biosynthesis |
Arabidopsis thaliana |
| single-base pair mutations in CesA genes |
result in decreased |
cellulose accumulation in primary or secondary cell walls |
|
| cellulose biosynthesis inhibitor (CBI) named ES20 |
likely targets |
catalytic site of (CESA6, E112, IXR2, PRC1, AT5G64740) |
|
| isoxaben |
may have |
another binding site on the transmembrane region on the extracellular side |
|
| dynamic complexes |
deposit |
cellulose |
|
| endosidin20-1 (ES20-1) |
might have another target site at |
transmembrane regions of cellulose synthase 6 (CESA6, E112, IXR2, PRC1, AT5G64740) |
|
| fluorescently labeled CESA |
localizes to |
plasma membrane (PM) |
|
| 14 out of 15 mutants (except es20r13) |
show reduced sensitivity to |
ES20-4 treatment |
Arabidopsis thaliana |
| careful examination of fluorescence-tagged CESAs with different mutations |
is required to define |
how different domains/motifs affect CSC trafficking |
|
| ES20-1 |
shows similar potency in |
bacteria at the concentrations tested |
|
| cellulose synthase complex |
deposits |
cellulose |
|
| isoxaben |
causes aberrant patterns in |
CELLULOSE SYNTHASE complex (CESA-C) |
|
| expression of (CESA4, IRX5, NWS2, AT5G44030) (ATCESA7, CESA7, IRX3, MUR10, AT5G17420) and (ATCESA8, CESA8, IRX1, LEW2, AT4G18780) |
was induced in |
PdMYB128 overexpression lines |
Arabidopsis thaliana |
| es20r5 and es20r13 mutants |
show similar sensitivity to |
ES20-2 treatment |
Arabidopsis thaliana |
| ES20-insensitive mutants |
showed reduced sensitivity to |
ES20-3 |
|
| 14 out of 15 ES20-insensitive mutants (except es20r13) |
showed reduced sensitivity to |
ES20-9 |
|
| an amino acid (R826-CESA3R806) in IF2 |
is required for |
the inhibitory effect of isoxaben |
|
| transgenic plants expressing (CESA6, E112, IXR2, PRC1, AT5G64740) with missense mutations at the catalytic site |
show reduced sensitivity to |
ES20-3 treatment |
Arabidopsis thaliana |
| Endosidin20 (ES20) |
targets |
cellulose synthase (CESA) |
|
| isoxaben |
is less clear for |
the relationship between predicted binding sites and amino acids required for inhibitory effect |
|
| another binding site on the transmembrane region on the extracellular side |
is surrounded by |
four amino acids whose mutations cause insensitivity to isoxaben |
|
| cellulose synthase complexes |
located in |
cell membrane |
|
| over-expression of (ATBRI1, BIN1, BRI1, CBB2, DWF2, AT4G39400) |
cannot completely rescue |
dwarf phenotype of some CESA mutants |
Arabidopsis thaliana |
| BRI1-GFP over-expression line |
has CESA1 expression much higher than |
wild type |
Arabidopsis thaliana |
| brassinosteroids (BRs) |
control through regulating expression of |
different sets of CESA genes |
Arabidopsis thaliana |
| bacteria |
produce |
cellulose |
|
| CBIs |
are believed to directly target |
cellulose synthases |
|
| CELLULOSE SYNTHASE (CESA) |
is |
important enzyme for cellulose biosynthesis in fibers |
Boehmeria nivea |
| isoxaben |
is |
widely used tool to dissect the mechanism of cellulose biosynthesis |
|
| UDP-glucose |
interacts with |
CESA6c (central cytosolic domain of (CESA6, E112, IXR2, PRC1, AT5G64740) ) |
Arabidopsis thaliana |
| BR signal in light |
can regulate expression of |
(CESA10, AT2G25540) |
Arabidopsis thaliana |
| 35S-BES1-GFP over-expression line |
has cellulose content 3% higher than |
wild type |
Arabidopsis thaliana |
| other growth-promoting hormones |
do not have broad effect on expression of |
CESA genes |
Arabidopsis thaliana |
| fluorescently labeled CESA |
localizes to |
CSC-containing vesicles known as microtubule-associated CESA compartments (MASCs) |
|
| prc1-1 mutant |
is |
null mutant of (CESA6, E112, IXR2, PRC1, AT5G64740) |
Arabidopsis thaliana |
| VIGS-NbCesA6 |
does not show major reduction of |
crystalline cellulose in stems |
|
| BRI1-GFP over-expression line |
has CESA2 expression much higher than |
wild type |
Arabidopsis thaliana |
| (CESA5, MUM3, AT5G09870) |
expression levels were hardly detected without |
BR treatment |
Arabidopsis thaliana |
| BR signal |
can regulate expression of |
(CESA5, MUM3, AT5G09870) |
Arabidopsis thaliana |
| re-equilibration of oxygen with stem water |
alters |
18 O enrichment which has already occurred in leaves |
|
| ES20-1 |
is more potent inhibitor of |
cellulose biosynthesis |
Arabidopsis thaliana |
| conserved DDG, DXD, TED, and QXXRW motifs |
are required for |
cellulose biosynthesis activity |
|
| es20r5 and es20r12 mutants |
show reduced sensitivity to |
ES20-6 treatment |
Arabidopsis thaliana |
| cellulose biosynthesis processes |
occur in |
higher plants |
|
| det2-1 mutant |
has cellulose content 8% lower than |
wild type |
Arabidopsis thaliana |
| (CESA10, AT2G25540) |
expression was not detected in |
young seedlings |
Arabidopsis thaliana |
| (CESA6, E112, IXR2, PRC1, AT5G64740) mutants |
are dramatically dwarfed or seedling-lethal |
dwarfed or seedling-lethal phenotype |
Arabidopsis thaliana |
| BRI1-GFP over-expression line |
has CESA7 expression not significantly enhanced in primary inflorescence stems at Stage V compared to |
wild type |
Arabidopsis thaliana |
| BRs |
promote |
expression of most CESA genes |
Arabidopsis thaliana |
| BR signal |
can regulate expression of |
(ATCESA2, ATH-A, CESA2, AT4G39350) |
Arabidopsis thaliana |
| F954 residue |
is |
highly conserved among plant and bacterial cellulose synthases |
Arabidopsis thaliana |
| (ANY1, AtCESA1, CESA1, RSW1, AT4G32410) allele |
hinders |
root growth at restrictive temperatures (29–31 °C) |
Arabidopsis thaliana |
| (ANY1, AtCESA1, CESA1, RSW1, AT4G32410) roots |
were |
short |
Arabidopsis thaliana |
| Atcesa6 (CESA6, E112, IXR2, PRC1, AT5G64740) (ANY1, AtCESA1, CESA1, RSW1, AT4G32410) YFP– F954L lines |
had |
short roots |
Arabidopsis thaliana |
| cellulose |
is labelled upon |
13CO2 feeding in the light |
|
| (ANY1, AtCESA1, CESA1, RSW1, AT4G32410) S167E mutant |
is detrimental to |
cellulose deposition |
Arabidopsis thaliana |
| stimulating photosynthetic effect on cellulose synthesis |
might be underpinned by |
enzymatic phosphorylation |
Arabidopsis thaliana |
| missense mutations in CESAs |
have been found to cause |
reduced sensitivity to CBIs |
|
| the transmembrane channel |
is |
the place where the glucan chain is elongated and translocated |
|
| microtubule orientation |
used as proxy for |
newly deposited cellulose orientation |
Arabidopsis thaliana |
| G. monilis CESA gene |
contains |
catalytic sites |
Gracilaria monilis |
| (CESA6, E112, IXR2, PRC1, AT5G64740) mutation in AtCESA6 |
has been complemented with |
fluorescently tagged (CESA6, E112, IXR2, PRC1, AT5G64740) |
Arabidopsis thaliana |
| UDP–glucose (UDP–Glc) |
is substrate for |
cellulose synthesis |
|
| cellulose |
usually takes a significant portion of |
total biomass |
Arabidopsis thaliana |
| BRs |
may promote |
cellulose biosynthesis |
Arabidopsis thaliana |
| (ANY1, AtCESA1, CESA1, RSW1, AT4G32410) mutants |
are dramatically dwarfed or seedling-lethal |
dwarfed or seedling-lethal phenotype |
Arabidopsis thaliana |
| BR-induced expression of (ATCESA2, ATH-A, CESA2, AT4G39350) and (CESA5, MUM3, AT5G09870) in prc1-1 |
may promote |
cellulose synthesis in order to keep cell elongating in light |
Arabidopsis thaliana |
| Endosidin20 (ES20) |
show inhibitory effect on |
bacterial cellulose synthesis |
|
| ES20-1 to ES20-9 |
induce |
ectopic lignin accumulation |
Arabidopsis thaliana |
| ES20-1 |
interacts with |
CESA6c P595S (CESA6c with P595S mutation) |
Arabidopsis thaliana |
| ES20 analogs |
show |
cellulose biosynthesis inhibition |
|
| ES20-insensitive mutants |
showed reduced sensitivity to |
ES20-1 |
|
| microtubules |
direct trajectories of |
cellulose synthase complexes |
|
| det2-1 mutant |
has CESA gene expression reduced to about 50% of |
wild type |
Arabidopsis thaliana |
| (CESA10, AT2G25540) |
shows induction by |
2, 4-epi-brassinolide (epiBL) |
Arabidopsis thaliana |
| ES20 |
targets |
catalytic domain of CESA |
|
| subcellular trafficking of CSCs |
has been found to be affected by |
CBIs |
|
| (CESA5, MUM3, AT5G09870) |
has |
poorly understood function |
Arabidopsis thaliana |
| (ATCESA3, ATH-B, CESA3, CEV1, ELI1, IXR1, MRE1, AT5G05170) and (CESA5, MUM3, AT5G09870) |
expression was only detected in root or shoot tips following |
BR treatment |
Arabidopsis thaliana |
| isoxaben |
has direct effect on |
CELLULOSE SYNTHASE 6 (CESA6, E112, IXR2, PRC1, AT5G64740) |
|
| Endosidin20 (ES20) |
might have another target site at |
transmembrane regions of cellulose synthase 6 (CESA6, E112, IXR2, PRC1, AT5G64740) |
|
| cellulose biosynthesis inhibitor (CBI) named ES20 |
interferes with |
subcellular trafficking of cellulose synthase (CESA) complexes (CSCs) |
|
| increased photosynthesis |
represents smaller proportion of |
fixed CO2 |
|
| cellulose deposition in Arabidopsis rosettes |
probably does not stop along |
day/night cycle |
Arabidopsis thaliana |
| complicated regulatory mechanisms |
control |
cellulose biosynthesis and assembling in cell walls |
|
| (ATCESA2, ATH-A, CESA2, AT4G39350) |
transcript levels were just slightly induced by |
2, 4-epi-brassinolide (epiBL) treatment |
Arabidopsis thaliana |
| (ATCESA3, ATH-B, CESA3, CEV1, ELI1, IXR1, MRE1, AT5G05170) |
transcript levels were just slightly induced by |
2, 4-epi-brassinolide (epiBL) treatment |
Arabidopsis thaliana |
| bri1-301 mutant |
has CESA gene transcript levels with no significant difference before and after |
2, 4-epi-brassinolide (epiBL) treatment |
Arabidopsis thaliana |
| brassinosteroids (BRs) |
regulate different sets of |
CESA expression at different developmental stages |
Arabidopsis thaliana |
| suppression of cellulose synthesis |
disorganizes |
microfibril orientation |
|
| CESA CBM48 |
is very unlikely to bind |
nascent cellulose chain (β-1,4 glucan) |
Gracilaria monilis |
| 2, 4-epi-brassinolide (epiBL) treatment |
largely induces expression of |
(CESA5, MUM3, AT5G09870) |
Arabidopsis thaliana |
| commitment of fixed carbon to cellulose biosynthesis |
decreases as |
net CO2 assimilation increases |
|
| immature leaves with intense cellulose synthesis |
are not directly sustained by |
photosynthesis |
Arabidopsis thaliana |
| amorphous cellulose |
can be detected using |
Bacillus-derived carbohydrate binding module 28 (CBM28) |
Arabidopsis thaliana |
| UDP-glucose pool in developing wood |
may be derived from |
fructose moiety of sucrose via glucose-1-phosphate to UDP-glucose conversion catalyzed by UGPase |
Populus tremuloides |
| cellulose biosynthesis requiring massive amounts of UDPGlc |
requires |
enormous investment of ATP and carbon skeletons |
Arabidopsis thaliana |
| (COB, ATMG00220) gene family |
plays an essential role in |
cellulose deposition |
|
| shou4-3 shou4l-1 mutant mucilage |
contains |
elevated levels of cellulose |
Arabidopsis thaliana |
| secondary cell wall cellulose |
is made by |
(CESA4, IRX5, NWS2, AT5G44030) (ATCESA7, CESA7, IRX3, MUR10, AT5G17420) and (ATCESA8, CESA8, IRX1, LEW2, AT4G18780) |
Arabidopsis thaliana |
| reduced (FRK1, FRK2, AT2G31390) activity |
leads to reduction in proportion of |
cellulose |
Populus tremula × tremuloides |
| this region |
was found to be |
highly conserved among all CesAs of rice |
Oryza sativa |
| cellulose formation in newly developing stems |
involves cleavage of sucrose allowing re-equilibration of oxygen with |
surrounding stem water |
|
| OsCesA9 |
has highest sequence similarity with |
(ATCESA7, CESA7, IRX3, MUR10, AT5G17420) among 10 Arabidopsis CesAs |
Oryza sativa |
| CELLULOSE SYNTHASE (CESA) |
differential effects on transcript levels are validated in |
PtMYB1 and PtMYB8 transgenics |
Picea glauca |
| dichlobenil (DCB) |
causes aberrant patterns in |
CELLULOSE SYNTHASE complex (CESA-C) |
|
| thaxtomin A |
inhibits incorporation of [14C]-glucose into |
acid-insoluble cell wall fraction (cellulose) |
Arabidopsis thaliana |
| OsCesA9 |
shares |
79% sequence identity with (ATCESA7, CESA7, IRX3, MUR10, AT5G17420) |
Oryza sativa |
| BRI1-GFP over-expression line |
has CESA4 expression significantly enhanced in primary inflorescence stems at Stage V compared to |
wild type |
Arabidopsis thaliana |
| sucrose synthase |
plays important roles in |
cellulose biosynthesis |
Gossypium hirsutum |
| TMH5–6 linker region |
is predicted to localize to |
apoplast |
Arabidopsis thaliana |
| IF3–TMH7 region in BcsA |
functions as |
gating loop that regulates substrate access into the active site |
|
| (ANY1, AtCESA1, CESA1, RSW1, AT4G32410) allele |
supports |
normal CESA function at 22 °C |
Arabidopsis thaliana |
| transgenic tobacco lines with augmented UDP–Glc availability |
show no evidence for |
improved cellulose biosynthetic rate |
Nicotiana tabacum |
| cellulose synthesis complexes (CSCs) |
includes |
(CSI1, POM2, AT2G22125) |
|
| vtc mutants |
are affected in |
cellulose synthesis |
Arabidopsis thaliana |
| Arabidopsis |
has |
10 CESA isoforms |
Arabidopsis thaliana |
| hydrophobic segment of TMH5–6 linker |
is |
well-conserved |
Arabidopsis thaliana |
| wild-type plants or mutant lines complemented with wild-type (ANY1, AtCESA1, CESA1, RSW1, AT4G32410) |
developed |
long roots at restrictive temperature |
Arabidopsis thaliana |
| cellulose biosynthesis in primary cell wall |
has been suggested to involve mainly |
(ANY1, AtCESA1, CESA1, RSW1, AT4G32410) (ATCESA3, ATH-B, CESA3, CEV1, ELI1, IXR1, MRE1, AT5G05170) (CESA6, E112, IXR2, PRC1, AT5G64740) |
Arabidopsis thaliana |
| cellulose in secondary cell wall |
involves |
(CESA4, IRX5, NWS2, AT5G44030) (ATCESA7, CESA7, IRX3, MUR10, AT5G17420) (ATCESA8, CESA8, IRX1, LEW2, AT4G18780) |
Arabidopsis thaliana |
| gas-exchange coupled to instant sampling with liquid nitrogen |
used to examine |
13C-labelling of cellulose |
Arabidopsis thaliana |
| Thr124 in (CESA5, MUM3, AT5G09870) |
is |
novel phosphorylation site |
Arabidopsis thaliana |
| cortical microtubules |
guide movement of |
cellulose-synthesizing enzyme complexes in plasma membrane |
|
| thaxtomin A |
affects |
distribution of CELLULOSE SYNTHASE complex (CESA-C) in plasma membrane |
Arabidopsis thaliana |
| cellulose synthase interacting protein 1 (CSI1, POM2, AT2G22125) |
participates in |
cellulose synthase complex (CSC) function |
Arabidopsis thaliana |
| isoxaben |
changes |
CESA motility |
|
| cellulose-formed microfibrils |
consist of |
linear chain of β-1,4-linked glucan |
|
| (ATCESA3, ATH-B, CESA3, CEV1, ELI1, IXR1, MRE1, AT5G05170) |
expression levels were hardly detected without |
BR treatment |
Arabidopsis thaliana |
| ct-2, ct-5 and ct-9 knockout mutants |
are functionally redundant with |
other CESA genes |
Arabidopsis thaliana |
| (ANY1, AtCESA1, CESA1, RSW1, AT4G32410) 3, and 6 genes |
have pairwise Pearson coefficient r >0.9 and form |
primary wall cellulose synthase complex (CSC) |
Arabidopsis thaliana |
| isoxaben |
inhibits |
cellulose biosynthesis |
Arabidopsis thaliana |
| enhanced density of CESA complexes at the PM in shou4-3 shou4l-1 seedlings |
leads to increase in |
amorphous cellulose |
|
| cell wall |
uses |
sucrose for cellulose synthesis |
|
| dichlobenil (DCB) |
causes aberrant CESA complex patterns in |
plasma membrane |
|
| two realignments from random to longitudinal microfibril orientation |
resulted from |
active deposition of longitudinally oriented microfibrils |
|
| OsCesA4 and OsCesA7 |
are expected to participate in |
cellulose synthesis in secondary cell walls, together with BC6 (OsCesA9) |
Oryza sativa |
| (ATCESA3, ATH-B, CESA3, CEV1, ELI1, IXR1, MRE1, AT5G05170) mutant |
is affected in |
cellulose synthase |
Arabidopsis thaliana |
| SUS gene |
may play important roles in |
cell wall cellulose synthesis |
|
| YFP-CESA6 in CPK32ΔC K96M seedlings |
had 44.0% remaining after 36 h cycloheximide treatment |
protein stability |
Arabidopsis thaliana |
| rosettes |
are visualized in |
Golgi cisternae |
|
| regulation of CESAs |
involves |
different kinases |
|
| CPK32ΔC transgenic plants |
had comparable |
crystalline cellulose content |
Arabidopsis thaliana |
| phosphorylation |
may regulate |
formation of CSCs, protein stability, and/or activation of CSCs at plasma membrane |
Arabidopsis thaliana |
| (ATCPK32, CDPK32, CPK32, AT3G57530) variants |
do not impact interaction with |
(ATCESA3, ATH-B, CESA3, CEV1, ELI1, IXR1, MRE1, AT5G05170) |
|
| (ATCPK32, CDPK32, CPK32, AT3G57530) |
influences |
cellulose biosynthesis |
|
| (ANY1, AtCESA1, CESA1, RSW1, AT4G32410) F954L expression in Atcesa6 (CESA6, E112, IXR2, PRC1, AT5G64740) YFP– line |
resulted in |
short roots at restrictive temperature |
Arabidopsis thaliana |
| Atcesa6 (CESA6, E112, IXR2, PRC1, AT5G64740) (ANY1, AtCESA1, CESA1, RSW1, AT4G32410) YFP– seedlings containing only allele of |
had |
short roots |
Arabidopsis thaliana |
| high CO2 conditions |
increases |
absolute carbon flux to cellulose deposition |
|
| UDP-glucose (UGP-Glc) |
is consumed by |
cellulose synthase complex (CSC) |
Arabidopsis thaliana |
| (ANY1, AtCESA1, CESA1, RSW1, AT4G32410) |
are highly co-expressed with |
(ATCESA3, ATH-B, CESA3, CEV1, ELI1, IXR1, MRE1, AT5G05170) |
Arabidopsis thaliana |
| sucrose synthase |
is |
candidate member of the cellulose synthase complex |
|
| phospho-dead mutation of (ATCESA3, ATH-B, CESA3, CEV1, ELI1, IXR1, MRE1, AT5G05170) |
leads to decreased |
motility of cellulose synthase complexes (CSCs) |
Arabidopsis thaliana |
| (ANY1, AtCESA1, CESA1, RSW1, AT4G32410) Ser 686 and Ser 688 |
reside in |
CSR domain |
|
| phospho-dead mutation of (ATCESA3, ATH-B, CESA3, CEV1, ELI1, IXR1, MRE1, AT5G05170) |
leads to reduced |
crystalline cellulose content in etiolated seedlings |
Arabidopsis thaliana |
| expression of RhCesA2 |
repressed by |
ethylene treatment of rose petals |
|
| phosphorylation of CELLULOSE SYNTHASE A (CESA) proteins |
is believed to exert key role in |
cellulose synthase complex (CSC) activity |
Arabidopsis thaliana |
| night-time cellulose deposition |
is catalysed by |
CESA5-containing cellulose synthase complex (CSC) |
Arabidopsis thaliana |
| CPK32ΔC K96M |
impairs phosphorylation of |
(ATCESA3, ATH-B, CESA3, CEV1, ELI1, IXR1, MRE1, AT5G05170) |
|
| (ATCPK32, CDPK32, CPK32, AT3G57530) |
directly interacts with catalytic domains of |
(ANY1, AtCESA1, CESA1, RSW1, AT4G32410) and (ATCESA3, ATH-B, CESA3, CEV1, ELI1, IXR1, MRE1, AT5G05170) |
|
| CESA |
localizes to |
various subcellular compartments |
|
| GhCesA4 |
expression was decreased in |
transgenic fibres with dominant repression of GhKNL1 |
Gossypium hirsutum |
| Atcesa6 (CESA6, E112, IXR2, PRC1, AT5G64740) (ANY1, AtCESA1, CESA1, RSW1, AT4G32410) YFP– line |
contains |
rsw1-1 allele |
Arabidopsis thaliana |
| cellulose biosynthesis in stomatal cells |
is |
strictly light-dependent |
Vicia faba |
| three CESAs (1, 3, and 5) |
are represented by |
8 phosphopeptides |
|
| (ANY1, AtCESA1, CESA1, RSW1, AT4G32410) F954L transgenic line |
generated in |
(ANY1, AtCESA1, CESA1, RSW1, AT4G32410) and Atcesa6 (CESA6, E112, IXR2, PRC1, AT5G64740) YFP– backgrounds |
Arabidopsis thaliana |
| short roots of Atcesa6 (CESA6, E112, IXR2, PRC1, AT5G64740) (ANY1, AtCESA1, CESA1, RSW1, AT4G32410) YFP– F954L lines |
indicates |
(ANY1, AtCESA1, CESA1, RSW1, AT4G32410) is non-functional when F954L mutation is present |
Arabidopsis thaliana |
| small punctate structures in plasma membrane |
are |
typical results for imaging active CESAs in plasma membrane |
Arabidopsis thaliana |
| CESA phosphorylation |
may down-regulate |
cellulose synthase complex (CSC) activity |
|
| THE1-GFP fusion protein overexpression |
did not influence |
cellulose deficiency |
Arabidopsis thaliana |
| (CESA5, MUM3, AT5G09870) |
presumably replaces |
(CESA6, E112, IXR2, PRC1, AT5G64740) in cellulose synthase complex (CSC) in darkness |
Arabidopsis thaliana |
| TMH5–6 linker region |
is |
longest predicted TMH linker among CESAs |
Arabidopsis thaliana |
| transgenic plants overexpressing OsNMD3 ΔNLS |
show reduced |
cellulose biosynthesis level |
Oryza sativa |
| cellulose synthase interacting protein 1 (CSI1, POM2, AT2G22125) |
makes bridge between |
microtubules and CELLULOSE SYNTHASE A (CESA) proteins |
Arabidopsis thaliana |
| thaxtomin A |
has clear impact on |
motility of CELLULOSE SYNTHASE complex (CESA-C) |
Arabidopsis thaliana |
| expression of potentially disruptive (ANY1, AtCESA1, CESA1, RSW1, AT4G32410) F954L mutant |
occurred despite |
small punctate structures in plasma membrane at 22 °C |
Arabidopsis thaliana |
| higher number of CESA particles at the cell surface |
might lead to |
non-optimal synthesis of cellulose |
|
| expression of sucrose synthase from cotton (Gossypium hirsutum) in poplar wood |
caused an increase in |
proportion of cellulose |
Populus trichocarpa; Gossypium hirsutum |
| OsNMD3 ΔNLS transgenic lines |
showed reduced |
cellulose content |
Oryza sativa |
| photosynthesis |
increases |
absolute metabolic flux to cellulose |
|
| co-expression module |
contains |
cellulose synthase subunit gene |
Coffea arabica |
| available isotopic data |
suggest |
cellulose production is stimulated by light |
|
| CESA monomers |
form |
hexamer of hexamers |
|
| absolute carbon flux to cellulose |
increases as |
net CO2 assimilation increases |
|
| (CESA5, MUM3, AT5G09870) activity |
is prevalent under dark conditions and stimulated by |
phosphorylation |
Arabidopsis thaliana |
| phosphorylation effects in enzymes of sugar metabolism |
may impact |
cellulose biosynthesis |
Arabidopsis thaliana |
| (CESA5, MUM3, AT5G09870) |
found together with |
(ANY1, AtCESA1, CESA1, RSW1, AT4G32410) and (ATCESA3, ATH-B, CESA3, CEV1, ELI1, IXR1, MRE1, AT5G05170) |
Arabidopsis thaliana |
| carbohydrate metabolism genes in cluster 1 |
included |
genes associated with cellulose biosynthesis |
Panicum hallii |
| (CESA5, MUM3, AT5G09870) and (CESA6, E112, IXR2, PRC1, AT5G64740) |
gave type 2 distribution pattern confirming |
upregulation in flower primordia |
Arabidopsis thaliana |
| CESA transcript and protein levels |
were not elevated in |
shou4-3 shou4l-1 mutant |
Arabidopsis thaliana |
| cortical microtubules (CMTs) |
guide |
cellulose synthase complexes |
Arabidopsis thaliana |
| cellulose synthesis complexes (CSCs) |
are made of |
cellulose synthases (CESAs) and associated proteins |
|
| rsw1-1 mutant |
is characterized by |
A549V substitution in (ANY1, AtCESA1, CESA1, RSW1, AT4G32410) |
Arabidopsis thaliana |
| high CO2 conditions |
results in |
13C-flux into cellulose synthesis of 0.15±0.01 μmol 13C m–2 s–1 |
|
| phosphopeptide associated with cellulose synthase interacting protein 1 (CSI1, POM2, AT2G22125) |
was detected |
phosphoproteomics analysis |
Arabidopsis thaliana |
| DCAR_019754 (CESA-like gene) |
expression level is significantly induced after 3 h of treatment |
24-epibrassinolide (24-EBL) treatment |
Daucus carota |
| (AtMYB103, ATMYB80, MS188, MYB103, MYB80, AT5G56110) plants |
showed |
decrease in peaks corresponding to cellulose |
Arabidopsis thaliana |
| cellulose synthase complex (CSC) activity |
reaches maximum or is down-regulated as |
CO2 assimilation increases |
|
| 14C-glucose labelling |
showed |
cellulose biosynthesis in stomatal cells is strictly light-dependent |
Vicia faba |
| 13CO2 feeding |
enables examination of |
13C-labelling of cellulose |
Arabidopsis thaliana |
| flufenacet |
caused |
aggregation of CESA in interphase cells |
Arabidopsis thaliana |
| no significant interaction between CELLULOSE SYNTHASE 5 (CESA5, MUM3, AT5G09870) and SALT-OVERLY SENSITIVE 5 (FLA4, SOS5, AT3G46550) |
was detected |
in cesa5-1 sos5-2 double mutant for cellulose content |
Arabidopsis thaliana |
| changes in cellulose microfibril structure |
correlate with |
cellulose content |
Arabidopsis thaliana |
| mutant cesa proteins |
lack |
catalytic activity |
Arabidopsis thaliana |
| COBRA-like 6 ( (COBL6, AT1G09790) ) |
is co-expressed with |
(CESA09, CESA9, AT2G21770) |
Arabidopsis thaliana |
| disruption of (SHOU4, AT1G78880) and (SHOU4L, AT1G16860) |
results in elevated levels of |
amorphous cellulose |
Arabidopsis thaliana |
| shou4-3 shou4l-1 and (STL1, AT2G41770) (STL2, AT3G57420) mutants |
have contrasting phenotypes in |
sensitivity to isoxaben |
|
| GhCESA1 |
expression was decreased in |
transgenic fibres with dominant repression of GhKNL1 |
Gossypium hirsutum |
| T942I structural change in (ATCESA3, ATH-B, CESA3, CEV1, ELI1, IXR1, MRE1, AT5G05170) |
possibly hinders |
interaction with isoxaben herbicide |
Arabidopsis thaliana |
| baker's yeast invertase |
altered |
cellulose biosynthesis |
Nicotiana tabacum; Saccharomyces cerevisiae |
| high photosynthesis |
results in smaller proportion of fixed carbon allocated to |
cellulose biosynthesis |
|
| ES7 (endosidin 7) treatment |
did not cause discernible difference in |
glucose labeling of cellulose |
Arabidopsis thaliana |
| (ATCESA7, CESA7, IRX3, MUR10, AT5G17420) |
is involved in |
secondary cell wall biosynthesis |
Arabidopsis thaliana |
| UDP-glucose |
is produced by |
hydrolysis of sucrose via sucrose synthase (no fractionation) or invertase (fractionates against C13) |
|
| significant reduction in cellulose content |
observed in |
(A/N-InvG, CINV1, NIN2, AT1G35580) (A/N-InvI, CINV2, AT4G09510) seedlings |
Arabidopsis thaliana |
| crystalline cellulose deposition in columella of (CESA5, MUM3, AT5G09870) |
is reduced compared with |
wild-type seeds |
|
| CELLULOSE SYNTHASE2 |
is found in |
saccharification-related QTL |
Zea mays |
| individual CESA classes |
have similar roles in determining |
cellulose microfibril structure |
Arabidopsis thaliana |
| cellulose microfibrils |
are synthesized by |
plasma membrane (PM)-localized cellulose synthase (CESA) complexes (CSCs) |
Arabidopsis thaliana |
| variance of trajectory angles of CESA particles |
was smaller in |
shou4-3 shou4l-1 |
Arabidopsis thaliana |
| (SHOU4, AT1G78880) and (SHOU4L, AT1G16860) mutations |
suppress |
phenotypes of mutants with reduced cellulose |
Arabidopsis thaliana |
| (SHOU4, AT1G78880) and (SHOU4L, AT1G16860) |
modulate |
rate of CESA exocytosis |
|
| demand in cellulose synthesis associated with growth |
orchestrates |
cellulose synthesis in leaves |
|
| low CO2 conditions |
results in |
13C-flux into cellulose synthesis of 0.10±0.01 μmol 13C m–2 s–1 |
|
| Green Fluorescent Protein-Cellulose Synthase A (GFP-CESA3)-tagged cellulose synthase tracks |
show no preferential transverse orientation at |
outer periclinal surface of small but mechanically asymmetric cells |
|
| reduced levels of cellulose |
is characteristic of |
(CESA6, E112, IXR2, PRC1, AT5G64740) mutants |
|
| (ATCESA3, ATH-B, CESA3, CEV1, ELI1, IXR1, MRE1, AT5G05170) |
exhibits high expression in |
SAM (shoot apical meristem) and young flower |
|
| decreased cellulose levels |
results in |
hypersensitivity to isoxaben |
Arabidopsis thaliana |
| (FRK1, FRK2, AT2G31390) |
is central for directing carbon to |
cellulose biosynthesis |
|
| (ATCESA3, ATH-B, CESA3, CEV1, ELI1, IXR1, MRE1, AT5G05170) (cellulose synthase catalytic subunit 3) |
is one of |
three isoforms necessary to obtain functional cellulose synthase complex |
Arabidopsis thaliana |
| rsw1-1 mutant |
is |
genetic mutant |
Arabidopsis thaliana |
| (SHOU4, AT1G78880) (SHOU4L, AT1G16860) mutant |
is less sensitive to |
cellulose inhibitor isoxaben |
Arabidopsis thaliana |
| rHB15-OE inflorescence stems |
exhibits significantly downregulated |
(CESA4, IRX5, NWS2, AT5G44030) |
Arabidopsis thaliana |
| CIN12 RNAi lines |
had lower |
cellulose levels |
Populus tremula × tremuloides |
| cellulose biosynthesis |
is hindered by |
insufficient UDP-glucose generation in (A/N-InvG, CINV1, NIN2, AT1G35580) (A/N-InvI, CINV2, AT4G09510) seedlings |
Arabidopsis thaliana |
| P557 of (ATCESA7, CESA7, IRX3, MUR10, AT5G17420) |
corresponds to |
P586 of OsCesA9 |
Oryza sativa |
| cellulose synthase inhibitor 2,6-dichlorobenzonitrile |
mimics effect of |
(ATCESA3, ATH-B, CESA3, CEV1, ELI1, IXR1, MRE1, AT5G05170) mutant |
Arabidopsis thaliana |
| PIF4-OE/hb15 stems |
exhibits higher expression of |
(CESA4, IRX5, NWS2, AT5G44030) |
Arabidopsis thaliana |
| (AtPIF4, PIF4, SRL2, AT2G43010) /rHB15-OE stems |
exhibits more significantly downregulated |
(CESA4, IRX5, NWS2, AT5G44030) |
Arabidopsis thaliana |
| cytosolic UDP-glucose concentration |
is well in excess of |
reported Km of 30 μM for secondary wall CESA from Populus tremula × tremuloides |
Arabidopsis thaliana; Populus tremula × tremuloides |
| aberrant microtubule organization |
propagated in |
CMF organization during microtubule-directed cellulose biosynthesis |
Arabidopsis thaliana |
| (AtMYB6, MYB6, AT4G09460) overexpression line (35S: ) |
shows no significant difference in |
cellulose content |
Populus tomentosa |
| (CESA5, MUM3, AT5G09870) and (CESA6, E112, IXR2, PRC1, AT5G64740) |
exhibiting higher mRNA levels in |
flowers |
Arabidopsis thaliana |
| cellulose content in PdMYB10 overexpression lines |
slightly increased by |
18% |
Arabidopsis thaliana |
| 14C commitment to cellulose biosynthesis in dark |
is |
2–3 times less than in light |
Glycine max |
| cellulose biosynthesis in intact cotton fibres |
is not correlated to |
UDP–Glc |
Gossypium hirsutum |
| gas exchange coupled to isotopic techniques |
used to examine |
cellulose biosynthesis under different photosynthetic contexts |
|
| stimulating photosynthetic effect on cellulose synthesis |
is driven by |
sugar metabolism |
Arabidopsis thaliana |
| rsw2-1 mutant |
is |
genetic mutant |
Arabidopsis thaliana |
| (FEI1, AT1G31420) (FEI2, AT2G35620) mutations |
suppress |
shou4-3 shou4l-1 dwarf phenotype |
|
| xyloglucan |
would allow |
random insertion of XXXG |
Nicotiana tabacum |
| cells dehabituated to DCB |
were able to restore |
normal levels of cellulose |
|
| methods for analysis of dynamics of cellulose deposition |
are in place for |
analysis of dynamics of cellulose deposition |
|
| DCB |
affects |
cellulose content |
Zea mays |
| sitosterol-β-glucoside |
can be used as primers for |
cellulose synthesis in vitro |
|
| overexpression of another mung bean sucrose synthase (SuSy) isoform in poplar |
did not increase |
amount of cellulose |
Populus trichocarpa |
| overexpression of UDP-glucose pyrophosphorylases (UGPases) in tobacco |
did not change |
cellulose production |
Nicotiana tabacum |
| PpCESA5 |
is |
CELLULOSE SYNTHASE (CESA) gene |
Physcomitrella patens |
| (FEI1, AT1G31420) (FEI2, AT2G35620) mutant roots |
are hypersensitive to |
isoxaben |
Arabidopsis thaliana |
| high temperature |
significantly suppresses expression of |
(CESA4, IRX5, NWS2, AT5G44030) |
Arabidopsis thaliana |
| decrease in (FRK1, FRK2, AT2G31390) activity |
correlated with |
decreased UDP-glucose levels |
|
| GFP-CESA5 in wild-type background |
appeared as |
small punctae occasionally arranged in longer striations perpendicular with outer surface of seed coat |
Arabidopsis thaliana |
| CesA subfamily |
comprises |
seven major lineages |
|
| (ATGH9A1, DEC, GH9A1, IRX2, KOR, KOR1, RSW2, TSD1, AT5G49720) |
encodes |
membrane-bound endo-1,4-D-glucanase |
Arabidopsis thaliana |
| AtCesA4 (CESA4, IRX5, NWS2, AT5G44030) (ATCESA7, CESA7, IRX3, MUR10, AT5G17420) and (ATCESA8, CESA8, IRX1, LEW2, AT4G18780) |
do not affect |
cellulose biosynthesis in primary cell walls |
Arabidopsis thaliana |
| (ATCPK32, CDPK32, CPK32, AT3G57530) |
is identified as |
putative interaction partner of catalytic domain of (ATCESA3, ATH-B, CESA3, CEV1, ELI1, IXR1, MRE1, AT5G05170) (CESA3CD) |
Arabidopsis thaliana |
| GFP-CESA3 S671A T672A transgenic lines |
had impaired |
CSC motility |
|
| (ATCPK32, CDPK32, CPK32, AT3G57530) |
influences |
CSCs motility |
|
| cortical microtubule (CMT) arrays |
guide the trajectories of |
cellulose synthase |
plant |
| rosette complex of CESA proteins |
facilitate |
synthesis of cellulose |
|
| fluorescent protein tagging of CESA |
identified |
CESA localization in small intracellular compartments |
|
| (ATCPK32, CDPK32, CPK32, AT3G57530) |
directly interacts with |
catalytic domain of (ANY1, AtCESA1, CESA1, RSW1, AT4G32410) (CESA1CD) |
Arabidopsis thaliana |
| cpk32-1 mutant |
had comparable |
crystalline cellulose content |
Arabidopsis thaliana |
| different CESA proteins |
form |
rosette complex |
|
| defective endocytosis in RADIAL SWELLING9 (ADL1, ADL1A, AG68, DL1, DRP1A, RSW9, AT5G42080) plants |
results in |
cellulose deficiency |
Arabidopsis thaliana |
| phospho-mimic glutamine (E) or phospho-dead alanine (A) |
revealed regulation of |
CESA via post-translational modification |
|
| GFP-CESA3 S671A T672A transgenic plants |
had |
decreased CSC motility |
Arabidopsis thaliana |
| (ATCPK32, CDPK32, CPK32, AT3G57530) |
facilitates specific phosphorylation of |
(ATCESA3, ATH-B, CESA3, CEV1, ELI1, IXR1, MRE1, AT5G05170) T672 |
|
| rosettes |
are visualized in |
vesicles |
|
| catalytic domain of (ATCESA3, ATH-B, CESA3, CEV1, ELI1, IXR1, MRE1, AT5G05170) (CESA3CD) |
used as bait to search for |
putative kinases that phosphorylate CESAs |
Arabidopsis thaliana |
| catalytic domain of CESAs |
directly interacts with |
kinase domain of (ATCPK32, CDPK32, CPK32, AT3G57530) |
Arabidopsis thaliana |
| RU04718 |
strongly repressed following |
ethylene treatment |
|
| RhCesA2 |
encodes |
putative cellulose synthase of 1,094 amino acids |
|
| (CESA4, IRX5, NWS2, AT5G44030) (ATCESA7, CESA7, IRX3, MUR10, AT5G17420) and (ATCESA8, CESA8, IRX1, LEW2, AT4G18780) in Arabidopsis |
contribute to |
cellulose synthesis for secondary cell wall |
Arabidopsis thaliana |
| CESAs |
localize to |
small CESA-containing compartments (SmaCCs) |
|
| (CESA6, E112, IXR2, PRC1, AT5G64740) |
found together with |
(ANY1, AtCESA1, CESA1, RSW1, AT4G32410) and (ATCESA3, ATH-B, CESA3, CEV1, ELI1, IXR1, MRE1, AT5G05170) |
Arabidopsis thaliana |
| (ATCESA3, ATH-B, CESA3, CEV1, ELI1, IXR1, MRE1, AT5G05170) |
encodes |
core cellulose subunit |
|
| Deregulation of calcium-dependent protein kinases (CPKs) |
impacted |
stability of cellulose synthase complexes (CSCs) |
Arabidopsis thaliana |